| detection and evaluation of feline oncornavirus-induced cell surface antigen(s) shed from cells in vitro. | a method for preparation of soluble feline oncornavirus-induced cell surface antigens was described. this technique relied upon the natural release of antigen(s) from fl-74 feline lymphoblastoid cells during their maintenance at 37 degrees in serum-deficient medium. when concentrated and clarified spent medium from 4-day cultures was tested for its antigen content by inhibition of humoral cytotoxicity, it was found that this natural production of soluble antigen provided more feline oncornaviru ... | 1977 | 198129 |
| protection of kittens from feline leukaemia virus infection by maternally-derived antibody. | | 1977 | 199981 |
| enhanced induction of growth of b lymphoblasts from fresh human blood by primate type-c retroviruses (gibbon ape leukemia virus and simian sarcoma virus). | | 1979 | 216638 |
| inhibition of concanavalin a stimulation of feline lymphocytes by inactivated feline leukemia virus. | in a lymphocyte blast transformation assay, the response of feline lymphocytes to concanavalin a was suppressed 20 to 65 percent in the presence of inactivated feline leukemia virus. the decrease was not due to viral cytotoxicity, as determined by trypan blue viability counts, nor was the virus binding the concanavalin a and interfering with its mitogenic stimulation. the virus may be biochemically repressive in itself, interfering with cell-mediated immunity within the feline system. | 1977 | 200353 |
| reiteration frequency of feline type c viral genomes in homologous and heterologous host cell dna. | differences in the multiplicity of proviral sequences of two feline endogenous viruses, rd-114 and felv, in homologous and heterologous host dna were examined by viral cdna hybridization using cellular dna fractionated with respect to reiteration frequency. the endogenous proviral dnas in cat cells were fractionated predominantly with intermediate-repeated sequences, while rd-114 proviral dna in virus-infected human cells was fractionated together with unique-sequence dna. these differences were ... | 1978 | 201591 |
| excretion of feline leukaemia virus by naturally infected pet cats. | | 1977 | 201852 |
| feline oncornavirus-associated cell membrane antigen: evidence for an immunologically crossreactive feline sarcoma virus-coded protein. | the feline oncornavirus-associated cell membrane antigen (focma) acts as a target for natural immuno-surveillance against tumor development in the cat. in the present study, mink and rat cells nonproductively transformed by feline sarcoma virus (fesv) were shown to express focma as well as 5'-terminal feline leukemia virus (felv) gag gene proteins, p15 and p12. in contrast, such cells lack detectable levels of other felv gag gene-coded proteins or the env gene product, gp70. focma, p15, and p12 ... | 1977 | 202959 |
| lysis of feline lymphoma cells by complement-dependent antibodies in feline leukemia virus contact cats. correlation of lysis and antibodies to feline oncornavirus-associated cell membrane antigen. | | 1978 | 203702 |
| cat leukemia and the cluster controversy. | | 1978 | 204405 |
| analysis of cytoplasmic rna and polyribosmomes from feline leukemia virus-infected cells. | cytoplasmic virus-specific rna and polyribosomes from a chronically infected feline thymus tumor cell line, f-422, were analyzed by using in vitro-synthesized feline leukemia virus (rickard strain) (r-felv) complementary dna (cdna) probe. by hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear rnas were found to be 2.1, 2.6, and 0.7% virus specific, respectively. size classes within subcellular fractions were determined by sucrose gradient centrifugation in the presence of di ... | 1978 | 205670 |
| pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (focma) activity. | feline sarcoma virus (fesv) rescued from transformed nonproducer mink or rat cells contains two fesv-specific antigens (p15 and p12), and the feline oncornavirus-associated cell membrane antigen (focma). all three antigens are helper virus-independent and are encoded by the fesv genome, focma, p15, and p12 antigens cochromatograph as phosphorylated molecules of 85,000 molecular weight (pp85), adsorb to immunoadsorbant columns prepared with antibodies to feline leukemia virus (felv), and are prec ... | 1978 | 206902 |
| characterisation of a tumour-specific antigen on the surface of feline lymphosarcoma cells. | | 1978 | 212681 |
| seroepidemiologic assessment of feline leukaemia virus infection risk for man. | | 1978 | 207994 |
| immunologic methods for the detection of humoral and cellular immunity. | a variety of immunologic techniques have been introduced during the past few years. many of these techniques are being applied to clinical specimens in an attempt to help the practicing veterinarian make a diagnosis. the introduction of new techniques requires extensive testing with clinically normal and diseased patients. it is essential for the practicing veterinarian to understand that the techniques available for the detection of immunologic disorders in the dog and cat are not routine diagn ... | 1978 | 220772 |
| influence of antisera to oncornavirus glycoprotein (gp71) on infections of cats with feline leukemia virus. | | 1978 | 208242 |
| differential growth and transmission in cats of feline leukaemia viruses of subgroups a and b. | the outcome of infecting cats with felv of sub-groups a (felv-a) or b (felv-b) was different. after felv-a infection, virus was quickly recovered from the blood and oropharynx of most animals. following infection with felv-b, only a small proportion of cats developed a viraemia, and this after a long interval. there was no evidence that felv-b was transmitted by contact. the result of infecting cats with mixtures of felv-a and b (felv-ab) was that to a large extent each virus operated independen ... | 1978 | 208983 |
| endogenous rd-114 virus of cats: absence of antibodies to rd-114 envelope antigens in cats naturally exposed to feline leukemia virus. | healthy cats, including those exposed to feline leukemia virus in leukemia cluster households, lack evidence of humoral immunity to virion surface antigens of the endogenous rd-114 virus. | 1979 | 222681 |
| potentiation of the neurtalization of feline leukaemia virus in hypotonic medium. | the neutralization of feline leukaemia virus could be enhanced by performing the reaction in hypotonic medium. absorption of antibody by virus specific antigens was also made more sensitive under these conditions. | 1978 | 211186 |
| [control of lymphosarcoma or leukaemia in cats and of feline leukaemia virus (author's transl)]. | a removal programme, specially designed for catteries or multiple cat households, to reduce the incidence of lymphosarcoma or leukaemia in cats and the spread of feline leukaemia virus (felv), is discussed. this removal programme includes annual testing of male stud cats, testing all contacts of felv-positive cats they had during the past two years, testing cats imported from abroad and isolation--or, if this is not possible, euthanasia--of cats found to be positive for felv-antigen. to detect t ... | 1978 | 211674 |
| diseases transmitted from pets to man: an evolving concern for veterinarians. | pets are not a major source of human infections but they can transmit certain diseases to man. this transmission usually is complex, requiring close contact with pets or their excretions and frequently involves a breach of sound hygienic practice. in some instances, pathogens of animal origin are acquired inadvertently because infectivity can persist after evidence of gross contamination has gone. veterinarians participate in controlling zoonotic diseases by encouraging rabies vaccination and h ... | 1978 | 213237 |
| detection of feline leukemia virus in tissues of cats by a paraffin embedding immunofluorescence procedure. | | 1978 | 213612 |
| group-specific cytolytic antibody directed against the major glycoprotein (gp70) of murine leukemia viruses in serum of mice with dormant flv infections. | | 1979 | 224581 |
| the control of lymphosarcoma/leukaemia and feline leukaemia virus. | | 1978 | 213655 |
| complement and tumor antibody levels in cats, and changes associated with natural feline leukemia virus infection and malignant disease. | | 1979 | 216484 |
| inhibition of human lymphocyte mitogen and antigen response by a 15,000-dalton protein from feline leukemia virus. | peripheral blood lymphocyte response of normal human subjects to mitogens and antigens was suppressed by a 15,000-dalton protein (p15) from a c-type feline leukemia virus. four of six subjects were suppressed 70 to 96% when responding to concanavalin a or phytohemagglutinin in the presence of 5.0 microgram of p15. the three subjects who responded to streptokinase-streptodornase and candida were suppressed 68 to 91% when cultured with 5.0 microgram of p15. the subviral protein did not appear to b ... | 1979 | 216488 |
| leukemia and lymphoma: infrequent manifestations of common viral infections? a review. | horizontally transmitted viruses have been etiologically linked to leukemia and lymphoma in several higher mammals (gibbons, cows, and cats). in the best-studied example, the cat, feline leukemia virus is a common community-acquired virus that infrequently produces cancer. however, the infection and its complications (cancer) are not typical of infectious diseases. epidemilogically, serologically, and virologically, infections with this type of virus can contradict classic infectious disease dog ... | 1978 | 216756 |
| research--the lifeline of veterinary medicine. | | 1978 | 216897 |
| infection and immunization of cats with the kawakami-theilen strain of feline leukemia virus. | | 1979 | 217021 |
| [feline leukemia virus (felv) i]. | | 1979 | 225795 |
| immunosuppressive properties of a virion polypeptide, a 15,000-dalton protein, from feline leukemia virus. | the 15,000-molecular-weight polypeptide (p15) of feline leukemia virus (felv) was shown to impair normal lymphocyte function in vitro and to abrogate immunity to feline oncornavirus disease in vivo. felvp15 suppressed concanavalin a-induced blast transformation of normal feline lymphocytes by 68%, while other virion proteins had no effect. p15 suppression was not due to toxicity, nor was p15 a competitive inhibitor of concanavalin a binding. capping of receptors for concanavalin a on normal feli ... | 1979 | 218725 |
| a feline leukaemia virus- and sarcoma virus-induced tumour-specific antigen. | | 1977 | 201856 |
| susceptibility of human cell lines to feline leukemia virus and feline sarcoma virus. | the susceptibility of human fibroblasts and human lymphoid cell lines to feline leukemia virus (felv) and feline sarcoma virus (fesv) was investigated. human cells were highly sensitive to infection by felv subgroups b and c and fesv but resistant to infection by felv subgroup a. the cells became infected, produced infectious virus, and displayed no differences in morphology or viability. t-cell lines appeared to be more sensitive to felv and fesv infection and to produce more virus than did aut ... | 1979 | 228103 |
| the occurrence of feline leukaemia virus neutralizing antibodies in cats. | | 1978 | 212375 |
| immuno-prophylaxis and -therapy of c-type oncorna viral diseases in mice and cats. | | 1977 | 202852 |
| mobility of lymphocyte surface membrane concanavalin a receptors of normal and feline leukemia virus-infected viremic felines. | the virus-associated depression of concanavalin a mitogenesis which accompanies feline leukemia virus-induced cat lymphoma was investigated by comparing lymphocyte surface receptor mobility of normal cats to that of viremic diseased animals. the mechanics of feline lymphocyte receptor mobility were studied using fluorescein-conjugated concanavalin a to quantitate lymphocyte capping. the results of a study of 21 disease-free animals showed that cat lymphocytes undergo appreciable concanavalin a c ... | 1979 | 218726 |
| recent developments in feline disease research. | | 1978 | 204451 |
| [feline leukemia: recent developments on the causes and immunoprevention of the disease (author's transl)]. | | 1978 | 230008 |
| the frequency of occurrence of feline leukaemia virus subgroups in cats. | feline leukaemia viruses (felv) were isolated from cats in glasgow and new york with lymphosarcoma and from apparently healthy carrier cats. the subgroup composition of each isolate was determined. all isolates contained felv of subgroup a(felv-a) and a high proportion also contained subgroup b virus (felv-b). virus of subgroup c (felv-c) was rare and occurred in association with felv-a and, in some isolates, with felv-b as well. the same pattern was observed in isolates from british and america ... | 1978 | 204584 |
| modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens. | observations and minor modifications are presented concerning the immunofluorescence assay for feline leukemia virus (felv) group-specific antigens (gsa) in blood cells of cats. data are given regarding absorption of goat felv gsa antiserum in vivo in cats, absorption of the antiserum in vitro with feline blood cells, and the comparative efficacy of various chemical fixatives in preservation of felv gsa for immunofluorescent staining. the best results were obtained with in vitro absorption of an ... | 1978 | 219732 |
| naturally occurring acquired immunodeficiency diseases of the dog and cat. | | 1978 | 220770 |
| applied viral immunology. | | 1978 | 220771 |
| feline preleukemia: an animal model of human disease. | | 1978 | 220806 |
| hypocomplementemia associated with naturally occurring lymphosarcoma in pet cats. | eighty cats were classified by indirect immunofluorescence and histologic diagnosis into four categories: normal, feline leukemia virus (felv) infected; normal noninfected; lymphosarcoma-felv infected; lymphosarcoma, no felv present. all viremic cats with lymphosarcoma were found to be hypocomplementemic and activation of the complement system had occurred via the classical pathway. sera of cats with lymphosarcoma in the absence of felv had varying levels of total hemolytic complement (tch50) ra ... | 1979 | 221581 |
| amino-terminal sequence of bovine leukemia virus major internal protein: homology with mammalian type c virus p30 structural proteins. | the amino acid composition, the cooh-terminal amino acid, and the nh2-terminal amino acid sequence of the first 55 residues of the major internal structural protein, p24, of bovine leukemia virus (blv) were determined. the compositional data and the results of end-group analysis revealed that, although blv p24 is chemically distinct, it more closely resembles the p30 structural proteins than the other gag gene products of mammalian retroviruses. it was found that blv p24 shares the common nh2-te ... | 1979 | 223166 |
| a simple method for quantitating salivary levels of virus using calcium alginate swabs. | | 1979 | 224079 |
| pathogenesis of experimental feline leukemia virus infection. | | 1979 | 224237 |
| involvement of different exogenous feline leukemia virus subgroups in the generation of independent feline sarcoma virus isolates. | | 1979 | 224571 |
| comparison of virus-positive and virus-negative cases of feline leukemia and lymphoma. | one hundred eighty-four cases of feline leukemia and lymphoma diagnosed in boston from 1972 through 1976 were investigated. fifty-eight % of these cases were lymphoma, and 42% were leukemia. sixty-seven % of the cats had positive fluorescent antibody tests for circulating feline leukemia virus. the rest (33%) were virus negative. clinically and epidemiologically, the virus-positive and virus-negative cases were remarkably similar except for their age at diagnosis. virus-negative cats tended to b ... | 1979 | 225010 |
| incorporation of hla antigens into the envelope of rna tumor viruses grown in human cells. | virion antigens of feline leukemia virus are associated with hla antigens in the membrane of human cells infected with the virus. feline leukemia virus produced by these cells incorporates host membrane antigens into the virion envelope, making the virus susceptible to lysis by antisera directed to hla or to uninfected cells plus complement. | 1979 | 225020 |
| influence of adrenal corticosteroids on the susceptibility of cats to feline leukemia virus infection. | the natural resistance of adult specific-pathogen-free cats to feline leukemia virus (felv) was abrogated by treatment with various doses of a synthetic corticosteroid, methylprednisolone acetate (mpa), prior to either oral-nasal or i.p. inoculation of felv. persistent viremia was induced in 82% (18 of 22) of mpa-treated cats versus 11% (1 of 9) of age-matched control cats. mpa-treated felv-inoculated cats developed prolonged lymphopenia (2 to 8 weeks postinoculation) and a delayed antibody resp ... | 1979 | 225025 |
| the demonstration of antibody specificity by a new technique. the gel electrophoresis-derived enzyme-linked immunosorbent assay (gedelisa) and its application to antibodies specific for feline leukemia virus. | by combining the high resolution of sodium dodecylsulfate polyacrylamide gel electrophoresis with the sensitivity of enzyme-linked immunosorbent assay (elisa) antibodies specific for different feline leukemia virus components are characterized. based on the same principle, concanavalin a binding sites of felv components are also detected. | 1979 | 225379 |
| structural studies of retroviruses: characterization of oligomeric complexes of murine and feline leukemia virus envelope and core components formed upon cross-linking. | to examine the protein proximity and subunit organization of type c retroviruses, preparations of akr murine leukemia virus were treated with bifunctional cross-linking reagents and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the cross-linked components obtained were characterized by immunoprecipitation with monospecific antisera against purified viral proteins, followed by sds-page analysis both before and after cleavage of the cross-links. with these proce ... | 1979 | 225513 |
| nature and distribution of feline sarcoma virus nucleotide sequences. | the genomes of three independent isolates of feline sarcoma virus (fesv) were compared by molecular hybridization techniques. using complementary dnas prepared from two strains, sm- and st-fesv, common complementary dna's were selected by sequential hybridization to fesv and feline leukemia virus rnas. these dnas were shown to be highly related among the three independent sarcoma virus isolates. fesv-specific complementary dnas were prepared by selection for hybridization by the homologous fesv ... | 1979 | 225544 |
| distribution of feline leukemia virus dna sequences in tissues of normal and leukemic domestic cats. | | 1979 | 227157 |
| simultaneous isolation of major viral proteins in one step. | | 1979 | 227293 |
| anemia associated with feline leukemia virus infection. | one hundred feline leukemia virus-positive cats with evidence of anemia were examined to determine characteristics of the anemia. the anemia was usually normochromic and normocytic, with low reticulocyte counts but with normal white blood cell and platelet counts. about one third of the cats had splenomegaly. the bone marrow was usually hypocellular or normally cellular, with an increased myeloid to erythroid ratio. a history of recent stress or infection in many cases indicated that the immunos ... | 1979 | 229095 |
| meeting report. report of meeting of section of clinical immunology and allergy, 11 march 1991. | | 1992 | 1311382 |
| hypercalcemia and proliferative, myelosclerotic bone reaction associated with feline leukovirus infection in a cat. | | 1979 | 229097 |
| restriction endonuclease mapping of unintegrated proviral dna of snyder-theilen feline sarcoma virus: localization of sarcoma-specific sequences. | extrachromosomal dna purified from mink cells acutely infected with the snyder-theilen strain of feline sarcoma virus (fesv) was digested with restriction endonucleases, and the dna fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled dna transcripts complementary to different portions of the fesv rna genome. major dna species 8.4 and 5.0 kilobase pairs (kbp) long represent the linear, unintegrated proviruses of snyder-theilen feline le ... | 1979 | 229270 |
| in-hospital felv testing. | | 1979 | 229397 |
| feline leukemia virus: survival under home and laboratory conditions. | feline leukemia virus maintained its infectiousness in cell culture medium at 37 degrees c or lower for at least 48 h. however, virus in saliva or medium was inactivated within a few hours if allowed to dry. | 1979 | 219019 |
| feline sarcoma virus-coded polyprotein: enzymatic cleavage by a type c virus-coded structural protein. | a purified 15,000-molecular-weight (mr) prague strain rous sarcoma virus gag gene-coded structural protein, p15, was shown to enzymatically cleave the previously described 130,000 mr feline sarcoma virus-coded polyprotein, pr130. cleavage products included proteins ranging in molecular weight from 12,000 to 110,000. the specificity of this cleavage reactivity was indicated by the fact that, under similar conditions, neither purified type c viral structural proteins nor nonviral proteins such as ... | 1979 | 219252 |
| recent trends in the study of oncornaviruses. | | 1979 | 231896 |
| [feline leukosis virus (felv). part ii]. | | 1979 | 232311 |
| studies of human serological reactivity with type c virus and viral proteins. | | 1979 | 232462 |
| recombination between feline leukemia virus subgroup b or c and endogenous env elements alters the in vitro biological activities of the viruses. | | 1992 | 1316496 |
| leukemia specific antigens: focma and immune surveillance. | in cats, horizontally transmitted viruses cause leukemia and lymphoma under natural conditions. as with other retroviruses, feline leukemia virus (felv) contains products of 3 major genes; the virus core gag gene products, the polymerase, and the virus envelope glycoprotein. when cells are transformed in vitro by the related feline sarcoma virus (fesv), an additional protein, focma is expressed at the cell membrane. focma, which is fesv-coded, is transformation and/or tumor specific and expresse ... | 1979 | 232469 |
| comparative analysis of rna tumor virus genomes. | | 1979 | 232474 |
| prevalence of feline immunodeficiency virus and other retroviral infections in sick cats in italy. | two hundred and seventy-seven sick pet cats living in italy were tested for antibodies to feline immunodeficiency virus (fiv) and for feline leukemia virus (felv) antigen. overall, 24% of the cats resulted positive for anti-fiv antibody and 18% for felv antigen. fiv was isolated from the peripheral mononuclear blood cells of ten out of 15 seropositive cats examined and from one out of eight saliva samples. no fiv isolations were obtained from six serum samples cultured. feline syncytium forming ... | 1992 | 1317073 |
| feline leukemia virus infections: the significance of chronic viremia. | | 1979 | 232893 |
| feline preventive medicine. | | 1979 | 233274 |
| hematologic abnormalities preceding myeloid leukemia in three cats. | cytopenia were recognized in three cats infected with feline leukemia virus. in one cat, marrow blast cells were increased in number, and a diagnosis of aleukemic leukemia was made. the disease progressed slowly for 3 1/2 months before terminating in acute myelomonocytic leukemia, recognized as a blast crisis in blood. in the other two cats, neutropenia and altered granulopoiesis in bone marrow preceded development of myeloid leukemia. | 1979 | 289225 |
| presence and expression of friend erythroleukemia virus-related sequences in normal and leukemic mouse tissues. | the nature and distribution of sequences related to the murine erythroleukemia virus, friend spleen focus-forming virus (sffv), have been analyzed by using a radioactive cdna probe specific for the sffv genome (cdna(sff)). from the proportion of high molecular weight viral [(32)p]rna which hybridized to cdna(sff), it was estimated that these sequences represent about 50% of the sffv genome, indicating a genetic complexity of about 3300 nucleotides. cdna(sff) hybridized extensively (80-95%) to sf ... | 1979 | 291976 |
| feline skin lymphoma: characterization of tumor and identification of tumor-stimulating serum factor(s). | a feline leukemia virus-negative skin lymphoma was characterized as a t-lymphocyte neoplasm, using the guinea pig erythrocyte rosetting technique. the lymphoma cells responded well to phytohemagglutinin compared with normal feline lymphocytes which did not respond. serum factor(s) was found in serum of a cat with lymphoma that was highly stimulating to autologous tumor cells, but not to normal cat lymphocytes. | 1979 | 316659 |
| comparative pathology of glomerulonephritis in animals. | glomerulonephritis constitutes an important category of renal diseases in animals and has been recognized with increasing frequency in the last decade. we report here the comparative morphologic aspects of glomerulonephritis as a naturally occurring disease of animals. we briefly review the immunopathogenesis of glomerulonephritis. the morphology of renal lesions occurring in glomerulonephritis in dogs, cats, cattle, sheep, horses and swine has been reviewed with emphasis on the range and specif ... | 1979 | 442447 |
| feline lymphocytes: observations on surface membrane concanavalin a receptor mobility. | the mechanics of concanavalin a receptor mobility of the feline lymphocyte surface membrane were investigated, utilizing fluorescein-labeled lectin to quantitate lymphocyte capping. the results of this study indicated that lectin concentration and buffer selection were critical for extensive receptor redistribution with cap formation of feline lymphocytes. maximal capping was obtained with 50 microgram of concanavalin a/ml of minimal essential medium. the mean capping rate of peripheral blood ly ... | 1979 | 507498 |
| the use of feline herpesvirus and baculovirus as vaccine vectors for the gag and env genes of feline leukaemia virus. | the env and gag genes from feline leukaemia virus were expressed in a thymidine kinase-negative feline herpes-virus and a baculovirus. cats were vaccinated with various combinations of these recombinant viruses and 100% protection against feline leukaemia virus challenge was achieved using an immunization schedule which utilized both env and gag products delivered at both a mucosal and systemic site. | 1992 | 1321214 |
| expression of feline leukaemia virus gp85 and gag proteins and assembly into virus-like particles using the baculovirus expression vector system. | in order to test components of feline leukaemia virus (felv) as subunit vaccines, we have constructed recombinant baculoviruses that express the felv envelope glycoprotein gp85 [autographa californica nuclear polyhedrosis virus (acnpv)-gp85] and the structural protein, gag (acnpvgag). the gag protein is expressed and shed into the medium of infected cells as particles which have a buoyant density on sucrose gradients and appearance by electron microscopy similar to those of authentic felv virion ... | 1992 | 1321215 |
| characterization of feline glomerulonephritis associated with viral-induced hematopoietic neoplasms. | light, electron, and immunofluorescence microscopy on tissues from 63 domestic cats revealed that glomerulonephritis occurred in almost one third of cats with hematopoietic neoplasms of the type linked with feline leukemia virus (felv). glomerular lesions were of the immune complex type with subepithelial, subendothelial, and mesangial dense deposits and reticular aggregates, similar to the nephropathy associated with systemic lupus erythematosus in humans. evidence that the glomerular lesions m ... | 1978 | 677265 |
| [kittens and felv]. | | 1992 | 1322571 |
| effect of feline panleukopenia virus infection on development of humoral and cellular immunity. | cats were infected with feline panleukopenia virus (fplv) at various ages pre- and post-parturition. all fetuses infected at 35 days of gestation from one queen and a single fetus infected at 45 days of gestation from a second queen had a significant delay in time of skin allograft rejection. older fetuses and newborn kittens infected with fplv did not show this delay in rejection time. adult cats infected with fplv demonstrated a significant decrease in lymphocyte responsiveness to two t cell m ... | 1976 | 782787 |
| experimental oncornavirus vaccines in the cat. | an experimental approach to the immunoprophylatic control of feline oncornavirus-mediated diseases has included induction of antivirus immunity and antibodies to the feline oncornavirus-associated membrane (tumor) antigens. a suitable model for exploring the effectiveness of killed oncornavirus vaccines in the cat has been provided by the use of feline sarcoma virus. immunization of seven pregnant queens over a 6-week period with ultraviolet light-inactivated gardner-arnstein feline sarcoma viru ... | 1976 | 943234 |
| feline leukemia virus subgroup b uses the same cell surface receptor as gibbon ape leukemia virus. | pseudotypes of gibbon ape leukemia virus/simian sarcoma-associated virus (galv/ssav) and feline leukemia virus subgroup b (felv-b) have been constructed by rescuing a moloney murine leukemia virus vector genome with wild-type galv/ssav or felv-b. the resulting recombinant viruses utilized core and envelope proteins from the wild-type virus and conferred resistance to growth in l-histidinol upon infected cells by virtue of the hisd gene encoded by the vector genome. they displayed the host range ... | 1992 | 1309898 |
| novel gacg-hairpin pair motif in the 5' untranslated region of type c retroviruses related to murine leukemia virus. | we searched for the presence of common rna structural motifs in mammalian type c retroviruses related to murine leukemia viruses and the closely related avian spleen necrosis virus. a novel motif consisting of a pair of hairpins, called hairpin pair motif, was detected in the 5' untranslated regions of the genomes of these retroviruses. a combination of computational analyses that included the assessment of phylogenetic sequence conservation by multiple alignment, the search for regions with unu ... | 1992 | 1309906 |
| feline leukemia virus: pathophysiology, prevention, and treatment. | | 1992 | 1312884 |
| detection of feline immunodeficiency proviral dna in peripheral blood lymphocytes by the polymerase chain reaction. | feline immunodeficiency virus (fiv) proviral dna was detected by the polymerase chain reaction method (pcr). pcr products were detected by gel electrophoresis and ethidium bromide staining. the p-10, p-15 and p-24 regions of the gag gene of fiv were chosen as the target sequences for amplification, and three primer pairs were prepared. the pcr products subjected to amplification with each primer pair were found to possess sites of digestion by a restriction enzyme, as hypothesized. they did not ... | 1992 | 1313618 |
| comparison of the viral promoter activities in feline cell lines (crfk and fcwf-4 cells). | | 1992 | 1313705 |
| insertional mutagenesis of flvi-2 in tumors induced by infection with lc-felv, a myc-containing strain of feline leukemia virus. | lc-felv is a myc-containing strain of feline leukemia virus (felv) which exhibits only partial transforming activity in vitro and in vivo. lc-felv infection in kittens may induce, but does not necessarily induce, thymic lymphosarcoma in viremic animals after a short latency. these observations suggest that infection with lc-felv is not sufficient to induce complete transformation and that another genetic event(s) is required. one possibility for such an event is that the integrating provirus act ... | 1992 | 1313907 |
| [feline leukemia complex]. | | 1979 | 230018 |
| nonhematopoietic hepatic neoplasms in cats: 21 cases (1983-1988). | nonhematopoietic hepatic neoplasms (n = 25) were diagnosed in 21 cats during a 5.5-year period. thirteen of the neoplasms were benign bile duct adenomas and 12 were malignant, 6 of which were bile duct adenocarcinomas. all cats were greater than or equal to 10 years old, and 14 were male. main clinical signs were anorexia and lethargy, and 15 of 21 cats had hepatomegaly. all 21 cats were feline leukemia virus-test negative. although there was a trend toward high activities of serum alanine trans ... | 1992 | 1330999 |
| safety and efficacy studies of live- and killed-feline leukemia virus vaccines. | the safety and the efficacy of several feline leukemia virus (felv) vaccines for 16-week-old kittens were determined. vaccines were derived from an fl74 lymphoblastoid cell line that has been in continuous tissue culture passage for about 4 years. the vaccines were made from living virus, formaldehyde-inactivated whole fl74 cells, and formaldehyde-inactivated whole virus. the efficacy of each produced vaccine was determined by challenge exposure of vaccinated cats with virulent felv. the two for ... | 1979 | 230767 |
| pathogenesis of feline leukemia virus t17: contrasting fates of helper, v-myc, and v-tcr proviruses in secondary tumors. | a naturally occurring feline thymic lymphosarcoma (t17) provided the unique observation of a t-cell antigen receptor beta-chain gene (v-tcr) transduced by a retrovirus. the primary tumor contained three classes of feline leukemia virus (felv) provirus, which have now been characterized in more detail as (i) v-tcr-containing recombinant proviruses, (ii) v-myc-containing recombinant proviruses, and (iii) apparently full-length helper felv proviruses. the two transductions appear to have been indep ... | 1992 | 1316466 |
| feline immunodeficiency virus: quantification in peripheral blood mononuclear cells and isolation from plasma of infected cats. | the titer of feline immunodeficiency virus in peripheral blood mononuclear cells (pbmc) and the presence of infectious virus in plasma was investigated over 20 week period in 8 experimentally infected cats, 3 uninfected cats and 2 naturally infected cats by end point dilution cultures using a feline t-lymphoblastoid cell line (mya-1). fiv was isolated from pbmc of all infected cats, but not from the uninfected cats. fiv was also isolated consistently from 100 microliters plasma from most of the ... | 1992 | 1333753 |
| identification of a putative receptor for subgroup a feline leukemia virus on feline t cells. | retrovirus infection is initiated by the binding of virus envelope glycoprotein to a receptor molecule present on cell membranes. to characterize a receptor for feline leukemia virus (felv), we extensively purified the viral envelope glycoprotein, gp70, from culture supernatants of felv-61e (subgroup a)-infected cells by immunoaffinity chromatography. binding of purified 125i-labeled gp70 to the feline t-cell line 3201 was specific and saturable, and scatchard analysis revealed a single class of ... | 1992 | 1316477 |
| structure and pathogenicity of individual variants within an immunodeficiency disease-inducing isolate of felv. | we previously described the molecular cloning of a replication-defective variant of feline leukemia virus (felv) that induced fatal immunodeficiency in cats. eighteen proviruses have now been molecularly cloned from cats inoculated with the original isolate (felv-faids) or its in vivo passages. three were replication-competent and each of these was noncytopathic for the feline t-cell line, 3201. replication of the prototype, felv-61e, in cats was associated with development of t cell tumors in s ... | 1992 | 1316674 |
| incidence of localized feline leukemia virus infection in cats. | anecdotal descriptions of atypical felv infections, wherein standard clinical elisa or immunofluorescence testing fails to detect active infections, suggest that an unknown proportion of felv-infected cats may go undetected. in this study, 127 viremic and nonviremic cats experimentally inoculated with felv were evaluated at necropsy for atypical expression of felv antigen. results from viremic cats were in accordance with results of earlier studies on the pathogenesis of felv infection in cats, ... | 1992 | 1316726 |
| a survey of fiv antibodies and felv antigens in free-roaming cats in the capital area of finland. | feline immunodeficiency virus (fiv) was first isolated and identified in 1986. since then it has been shown to have a worldwide distribution, and the infection generally appears to have reached a state of endemicity. this is the 1st study of fiv-prevalence in finland. serum samples of 196 free-roaming cats were tested for antibodies to fiv and felv antigens (feline leukemia virus). with a combined enzyme-linked immunosorbent assay (elisa), 13 of the cats (6.6%) turned out to be positive for fiv ... | 1992 | 1317991 |
| development of a monoclonal antibody-based p24 capsid antigen detection assay for htlv-i, htlv-ii, and stlv-i infection. | a monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay (elisa) was developed and employed to detect p24 capsid antigen from human t-cell lymphotropic viruses type i and ii (htlv-i, htlv-ii), simian t-cell lymphotropic virus type i (stlv-i)-infected cell lines, and from mononuclear cell cocultures of htlv-infected humans and stlv-i infected monkeys. a monoclonal antibody specific for htlv p24 and p53 capsid antigens was coated onto 96-well microtiter plates to capture htlv/ ... | 1992 | 1318063 |
| identification and evaluation of new primer sets for the detection of lentivirus proviral dna. | we have developed sets of degenerate oligonucleotides designed to detect pol gene sequences from any member of the lentivirus subfamily when used as primers in amplification techniques such as the polymerase chain reaction (pcr). this pan-lentivirus-specific primer set (plsps) consists of primers, lv1, lv2, and lv3, based on conserved regions common to lentiviruses only. our protocol is based on primary amplification with lv1 and lv2 followed by secondary amplification with a nested primer set b ... | 1992 | 1337258 |
| pathogenicity of feline leukemia virus is commonly associated with variant viruses. | many of the serious diseases resulting from feline leukemia virus (felv) infection are associated with the generation of novel variant viruses. the prototype felv-a virus is highly stable and circulates in the cat population without apparent antigenic change. however, recombination with cellular oncogenes produces viruses which cause leukemia or other malignant diseases. other recombinants within the env genes of felv-a and endogenous felv are recognized as belonging to a second subgroup, felv-b ... | 1992 | 1318467 |