| evaluation and simplification of the world health organization clinical case definition for paediatric aids. | the world health organization (who) clinical case definition for paediatric aids was tested during a 1-month period on 221 consecutive hospitalized children in kigali, rwanda. relevant clinical features not included in the who case definition were also evaluated. thirty-four out of the 221 children (15.4%) were hiv seropositive. although the specificity of the who case definition was high (92%), the sensitivity and the positive predictive value (ppv) were low (41 and 48%, respectively). the foll ... | 1989 | 2500955 |
| an enzyme-linked immunosorbent assay for detection of antibodies to porcine parvovirus. | an enzyme-linked immunosorbent assay (elisa) was developed for detection of antibodies to porcine parvovirus (ppv). antisera to ppv were raised in pigs, for which ppv grown on pk15 cells was used for primary intranasal inoculations, and ppv cultured on autologous kidney cells for booster immunisations. a competition elisa was developed, based on the principle of a double antibody sandwich assay, using immunoglobulin fractions prepared from these sera. the elisa was compared with a haemagglutinat ... | 1989 | 2542351 |
| characterization of a parvovirus isolated from the diarrheic feces of a pig. | a small dna virus was isolated from the feces of a sow with diarrhea and identified as a parvovirus on the basis of its properties. the virus replicated preferentially in cell cultures of swine origin, including primary porcine thyroid gland and kidney cell cultures in which the cytopathic effect developed. the virus agglutinated erythrocytes of guinea pig, mouse and human group o but not these of chicken. the growth of the virus was inhibited by 5-iodo-2'-deoxyuridine. the virus was resistant t ... | 1989 | 2544760 |
| immunogenicity of poliovirus b and t cell epitopes presented by hybrid porcine parvovirus particles. | we have analysed the potential capacity of hybrid porcine parvovirus (ppv) capsids to present foreign epitopes to the immune system. foreign sequences were introduced into the n and c termini of ppv vp2, which was previously shown to assemble spontaneously into parvovirus-like particles. the integrity of the c terminus was shown to be essential for preserving the structure of the capsid and therefore could not be used for epitope fusion. in contrast, insertion of sequences corresponding to t and ... | 1995 | 7561778 |
| in vitro assessment of viral antigen content in inactivated aluminum hydroxide adjuvanted vaccines. | antigen capture enzyme immunoassays (elisa) were developed to assess the antigenic content of inactivated aluminum hydroxide (ah) adjuvanted porcine parvovirus, pseudorabies, and infectious bovine rhinotracheitis vaccines. reference preparations of these viruses were constructed as a basis for comparison. because ah-associated elisa interference was largely circumvented, the need for isotopic or complex antigen-adjuvant desorption methods was eliminated. a 4-parameter logistic model related opti ... | 1989 | 2550498 |
| sensitive non-radioactive nucleic acid hybridization assay for plum pox virus detection. | a new non-radioactive sandwich hybridization assay was designed to simplify the analysis of a large number of plant samples. plant material was homogenized in 0.5% sds and added directly to the hybridization reaction, in which a pair of identifying probes were used. one of the probes was biotinylated capture rna specific for plum pox virus (ppv) strain sk-68; the other rna probe was synthesized from a plasmid bearing the adjacent sequence of this strain and was labelled with digoxigenin (dig). b ... | 1994 | 7709075 |
| adventitious pestivirus rna in live virus vaccines against bovine and swine diseases. | live virus vaccines against bovine and porcine diseases were examined for the presence of adventitious pestivirus rna or pestiviruses by reverse transcription-polymerase chain reaction (pcr). pestivirus rna was detected in the live virus vaccines against akabane disease, ibaraki disease, infectious bovine rhinotracheitis, porcine parvovirus infection, transmissible gastroenteritis and japanese encephalitis. pestivirus rna or pestivirus in the fetal bovine serum used to grow the host cells used t ... | 1995 | 7762264 |
| artificial cleavage site recognized by plum pox potyvirus protease in escherichia coli. | a synthetic plum pox virus (ppv) nib-cp cleavage site was recognized by a ppv protease in an in vivo escherichia coli expression system. the presence of the natural nib-cp cleavage site did not affect processing at the artificial one. however, although both the proteases and the cleavage sites of ppv and tobacco etch virus show high sequence homology, a similar cassette from the tobacco etch virus nib-cp junction was not efficiently recognized by the ppv protease. | 1989 | 2657098 |
| porcine parvovirus associated with cutaneous lesions in piglets. | | 1994 | 7948207 |
| return to oestrus after first insemination in sow herds (incidence, seasonality, and association with reproductivity and some blood parameters). | as no systematic study has been done to get an accurate estimate of the incidence of return to oestrus after first insemination in sows in the netherlands, the objectives of this investigation were: 1) to obtain an estimate of the incidence of return to oestrus after insemination at the herd level; 2) to investigate the association between incidence of return to oestrus after first insemination and reproduction characteristics in order to get an impression of the economic importance of reproduct ... | 1994 | 7985351 |
| a novel non-mineral oil-based adjuvant. ii. efficacy of a synthetic sulfolipopolysaccharide in a squalane-in-water emulsion in pigs. | the adjuvanticity of a sulfolipopolysaccharide (slp) incorporated into a squalane-in-water emulsion (slp/s/w) was compared with that of a mineral oil-in-water (o/w) adjuvant currently used in commercial porcine vaccines. groups of pigs were immunized twice with vaccines comprising either inactivated influenza virus (iflu3 containing strains a/swine, mrc-11 and x-79), inactivated pseudorabies virus (iprv), live pseudorabies virus (prv) or inactivated porcine parvovirus (ippv) as antigen and slp/s ... | 1994 | 8085386 |
| the complete nucleotide sequence of plum pox virus rna. | the complete nucleotide sequence of the rna of an aphid non-transmissible plum pox virus (ppv-nat) isolate has been determined from five overlapping cdna clones. cdna prepared by primer extension was used to determine the 5' terminus. the assembled rna is 9741 nucleotides in length, excluding a 3' terminal poly(a) sequence. one large open reading frame starts at nucleotide positions 36 to 38 and is terminated with an uag codon at positions 9522 to 9524. the putative start codon is located at pos ... | 1989 | 2732699 |
| [the infectious causes of abortion and stillbirth in swine in switzerland]. | fetuses and placentae of 171 cases of porcine abortion, stillbirth and mummification were examined for pathological lesions, bacterial infections and ppv (porcine parvovirus) infection. furthermore igg (immunoglobulin g) levels were determined in fetal body fluids. selected maternal sera were tested for antibodies against leptospira, aujeszky's disease and hog cholera. ppv infection was diagnosed in 29.2% of all cases. bacterial abortion was diagnosed in 8.2%. indications for an infectious agent ... | 1993 | 8128797 |
| the complete nucleotide sequence of plum pox potyvirus rna. | the complete nucleotide sequence of the plum pox virus (ppv) rna genome has been determined. the rna sequence is 9786 nucleotides in length, excluding the 3'-terminal poly(a) tail. an aug triplet at position 147-149 was assigned as the initiation codon for the translation of the genome size viral polyprotein which would consist of 3140 amino acid residues. the nucleotide sequence of the non-coding regions and the predicted amino acid sequence of the polyprotein of ppv were compared with those pr ... | 1989 | 2773595 |
| use of polymerase chain reaction to detect porcine parvovirus associated with swine embryos. | the role of porcine parvovirus (ppv) in inducing reproductive failure in swine has been extensively documented. however, information is not available as to the risk of ppv transmission by embryo transfer. using the polymerase chain reaction (pcr) technique, ppv-specific dna was detected in association with 4-day-old porcine embryos incubated in vitro in the presence of nadl-8 strain of ppv, despite attempts to rid the embryos of virus by either washing or treatment with pronase or trypsin. the p ... | 1994 | 8192255 |
| genomic organization and mapping of transcription and translation products of the nadl-2 strain of porcine parvovirus. | the nadl-2 strain of ppv was cloned into puc19 and independent infectious clones were sequenced. this permitted a correction of published sequences and to predict a cruciform structure as an alternative to the 5'-hairpin of the "-" strand. this 5'-end structural covariance is shared with other parvoviruses of the same group and two alternative sequences ("flip" and "flop") were present in the region of the cruciform. transcript and translation product mapping allowed the prediction of the locati ... | 1993 | 8212598 |
| monoclonal antibodies suitable for plum pox virus determination. | monoclonal antibodies (mabs) to plum pox virus (ppv) were obtained after immunization of balb/c mice with purified ppv-w isolate. spleen cells from a mouse showing a high serum titer were used for fusion with sp2/0-ag14 myeloma cells. culture supernatants were screened for specific antibody production against ppv-w isolate using indirect elisa. a total of six stable hybridoma lines producing mabs of igg class were obtained. all four ppv isolates tested (w, a, d and m) can be distinguished by the ... | 1993 | 8314598 |
| a new method for rapidly removing contaminating micro-organisms from porcine parvovirus or pseudorabies virus master-seed suspensions. | virus-contaminated cell cultures are a major problem in the bio-industry. methods employed to date to remove contaminating micro-organisms are slow and costly, and a new method is proposed here which is simple and rapid. the method uses polyacrylamide beads coated with specific antibodies which yielded bead-antibody-virus complexes when suspended in the virus solution to be cleared. the purified virus was propagated in cells which show phagocytic activity. vaccine master-seed virus is shown to b ... | 1993 | 8383386 |
| tissue tropisms of porcine parvovirus in swine. | late-term gestation swine fetuses, similar to adult animals, are able to effectively mount immune response and survive porcine parvovirus (ppv) infection. an exception to this is the kresse strain of ppv, which causes fetal death in late-term gestation swine fetuses. in an effort to understand the basis for this profound difference in pathogenicity between kresse strain and the prototype strain of ppv, nadl-8, studies were designed to examine potential difference in sites of replication and quan ... | 1993 | 8390826 |
| the complete nucleotide sequence of an infectious clone of porcine parvovirus, strain nadl-2. | the complete nucleotide sequence of an infectious clone of porcine parvovirus, strain nadl-2, was determined. the nucleotide sequence organization of the viral genome was found to be similar to that of the other autonomous parvoviruses, such as canine parvovirus and minute virus of mice. | 1990 | 2219713 |
| role of the tk+ phenotype in the stability of pigeonpox virus recombinant. | insertion of foreign dna containing the e. coli gpt marker by homologous recombination in the pigeonpox virus (ppv) thymidine kinase (tk) gene and selection for the presence of this dna in the viral genome produced unstable recombinants after 3 plaque purifications. we highlight the persistence of duplicated tk dna sequences arising from single crossing over, due to the growth advantage of tk+ virus. restoration of the tk function by coinsertion of the vaccinia virus tk gene led to stable tk+ re ... | 1993 | 8394070 |
| rna helicase: a novel activity associated with a protein encoded by a positive strand rna virus. | most positive strand rna viruses infecting plants and animals encode proteins containing the so-called nucleotide binding motif (ntbm) (1) in their amino acid sequences (2). as suggested from the high level of sequence similarity of these viral proteins with the recently described superfamilies of helicase-like proteins (3-5), the ntbm-containing cylindrical inclusion (ci) protein from plum pox virus (ppv), which belongs to the potyvirus group of positive strand rna viruses, is shown to be able ... | 1990 | 2263459 |
| evaluation of testing algorithms following the use of combination hiv-1/hiv-2 eia for screening purposes. | the licensure of combination human immunodeficiency virus type 1 and type 2 (hiv-1/hiv-2) enzyme immunoassays (eias) by the food and drug administration has been accompanied by a recommendation that u.s. blood banks begin testing the nation's blood supply for hiv-2 by june 1, 1992. the performance of a recently licensed combination hiv-1/hiv-2 eia (genetic systems) was evaluated using 3100 sera collected in the united states. a total of 2,049 sera were obtained from populations with low risk for ... | 1993 | 8457381 |
| [suppression of replication of swine parvoviral antisense rna against the ns ppv gene in swine thyroid gland cells]. | the possibility of suppression of porcine parvovirus (ppv) reproduction in the culture of thyroid gland cells of a swine that contain the integrated genes for asrna against the nonstructural proteins of the virus has been studied. 10 cell lines with the asrna genes have been obtained. the line with the maximal number of integrated gene copies was used to inflict with the parvovirus. the expression of asrna in this cell line was shown to lead to 95% suppression of ppv replication as compared with ... | 1993 | 8510680 |
| immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants. | tobacco plants (nicotiana tabacum cv. xanthi) have been transformed via agrobacterium tumefaciens vectors, with cdnas corresponding to the plum pox virus (ppv) cistron 2 encoding helper component (hc-pro) and with the first two and half cistrons of the ppv genome. presence of the hc-pro in ppv-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the ppv hc-pro. the results suggest that two p ... | 1993 | 8517789 |
| mutational analysis of plum pox potyvirus polyprotein processing by the nia protease in escherichia coli. | a binary escherichia coli expression system has been used to study the pathway for proteolytic processing of the plum pox potyvirus (ppv) polyprotein. trans cleavage at the carboxyl end of the cylindrical inclusion protein occurred, although with lower efficiency than that at the large nuclear inclusion protein-capsid protein junction. no trans cleavage at the carboxyl end of the small nuclear inclusion protein (nia) was detected. the proteolytic activities at different cleavage sites of several ... | 1990 | 2273380 |
| detection of respiratory syncytial virus by reverse transcription-pcr and hybridization with a dna enzyme immunoassay. | nasal aspirates from 238 infants hospitalized with acute respiratory infections during the winter of 1994 and 1995 were tested for respiratory syncytial virus (rsv) by immunofluorescence assay (ifa) and the viral isolation technique (vit) and by two pcr and hybridization methods: reverse transcription pcr 1 (rt-pcr1), which amplifies the rnas of all rsv strains, and rt-pcr-2, which allows subgroup classification of rsv. rt-pcr-1 and rt-pcr-2 detected viral sequences in 56.7% (135 of 238) and 48. ... | 1995 | 8586738 |
| financial evaluation of vaccination and testing alternatives for control of parvovirus-induced reproductive failure in swine. | to identify the preferable testing and vaccination strategy for control of porcine parvovirus (ppv) during a 6-month period. | 1996 | 8617643 |
| infectious in vitro transcripts from a plum pox potyvirus cdna clone. | a full-length cdna clone of the 9786 nt plum pox virus (ppv) rna genome has been cloned downstream from a phage t7 rna polymerase promoter. the rnas synthesized by in vitro run-off transcription in the presence of the 5' cap analog m7gpppg were infectious in nicotiana clevelandii plants. no infectivity was detected when the transcriptions were carried out in the absence of the cap analog. inoculations of the local lesion host chenopodium foetidum indicated that the infectivity of the synthetic t ... | 1990 | 2371774 |
| cultivation of a pig parvovirus in various cell cultures. | the susceptibility of several established cell lines of pig (llc-pk1 = pig kidney; mpk = minipig kidney; pk15 = pig kidney; esk = embryonic swine kidney), bovine (ebtr = embryonic bovine trachea), monkey (ma-104 = fetal rhesus monkey kidney) and human (hel-299 = embryonic human lung) origin to porcine parvovirus was studied. the primary pig kidney cell cultures (ppk) were included in the study as the reference cell system. from the results it appeared that the virus only replicated in cell lines ... | 1987 | 3626887 |
| print-capture pcr: a simple and highly sensitive method for the detection of plum pox virus (ppv) in plant tissues. | | 1996 | 8668554 |
| inactivation of virus during anaerobic digestion of manure in laboratory scale biogas reactors. | reduction of porcine parvovirus, bovine enterovirus and faecal enterococci were measured in biogas reactors continuously run on manure and manure supplemented with household waste at 35 degrees c and 55 degrees c and in batch test run at 70 degrees c. the aim of the experiments was to study the sanitation effect of anaerobic digestion and to evaluate the use of faecal enterococci as an indicator of sanitation. parallel studies on the reduction of virus and faecal enterococci were done in physiol ... | 1996 | 8678476 |
| the rna helicase ci from plum pox potyvirus has two regions involved in binding to rna. | the plum pox virus (ppv) protein ci is an rna helicase, whose function in the virus replication is still unknown. recently, an rna binding domain was mapped to a region of the ci protein that includes the arginine-rich motif vi typical of rna helicases of the superfamily sf2. in the present study, a second region involved in rna binding activity of the ci protein has been identified. northwestern assays with a series of maltose-binding protein fusions that contain different ci fragments showed t ... | 1996 | 8690088 |
| diagnosis of fetal infection with porcine parvovirus by in situ hybridization. | in situ hybridization (ish) for the diagnosis of fetal infection with porcine parvovirus (ppv) was compared with immune electron microscopy (iem) and serology by immunofluorescence (if) for its sensitivity and its applicability in a routine diagnostic laboratory. the technique was applied to the examination of sections of formalin-fixed paraffin-embedded tissues from 68 fetuses. fifty-three of these fetuses were diagnosed serologically since they had a crown rump length of more than 17 cm, i.e. ... | 1995 | 8748552 |
| porcine parvovirus: propagation in microcarrier cell culture and immunogenic evaluation in pregnant gilts. | porcine parvovirus was propagated in pk-15 cells cultured in roller bottles or on microcarrier beads. after inactivation, the virus was used as antigen in the preparation of vaccines. the immunogenic potency and safety of the vaccines were evaluated in specific pathogen free pregnant gilts and guinea pigs. experimental challenge tests determined the efficacy of the vaccine in preventing porcine parvovirus transplacental infection. neither viral antigens nor specific antibodies were detected in f ... | 1986 | 3809732 |
| comparison of the safety and immunogenicity of a pneumococcal conjugate with a licensed polysaccharide vaccine in human immunodeficiency virus and non-human immunodeficiency virus-infected children. | to compare the safety and immunogenicity of a 5-valent pneumococcal conjugate vaccine to a licensed 23-valent polysaccharide pneumococcal vaccine in hiv-infected and non-hiv-infected children > or = 2 years old. | 1996 | 8852905 |
| oronasal and intramuscular vaccination of swine with a modified live porcine parvovirus vaccine: multiplication and transmission of the vaccine virus. | an attenuated strain nadl-2 of porcine parvovirus (ppv) has been used at the 54th cell culture passage as a modified live-virus (mlv) vaccine. the present study was conducted to determine the minimum immunizing dose of mlv, the extent of mlv multiplication in swine tissues, and its transmission from swine administered mlv oronasally or intramuscularly. immune response to mlv was dose dependent and swine responded to as little as 10(2) median cell-culture infective doses (ccid50). a 10(5) ccid50 ... | 1984 | 6098202 |
| acute outbreak of porcine parvovirus infection in mozambique. | investigations were made to determine the causal agent of an acute outbreak of abortions recorded in a swine herd in mozambique. isolation of porcine parvovirus and demonstration of its specific antibodies accomplished by using enzyme-linked immunosorbent assay, haemagglutination inhibition and immunofluorescent tests, indicated that porcine parvovirus was the causal agent of the abortions. other pathogenic agents causing reproductive failure, e.g. pseudorabies virus, leptospira or brucella spec ... | 1995 | 8966762 |
| morphological and immunological comparison of caprine arthritis encephalitis and ovine progressive pneumonia viruses. | caprine arthritis encephalitis virus (caev) causes a variety of pathological conditions ranging from mild to very severe and from acute to chronic, depending upon the age of initial infection and other variables. although the virus has been reported to have properties of characteristic of retroviruses and to be related to maedi-visna virus (also called progressive pneumonia virus [ppv]), relatively little information about its morphological and immunological characteristics has been reported. we ... | 1981 | 6169845 |
| detection of antibodies against porcine parvovirus nonstructural protein ns1 may distinguish between vaccinated and infected pigs. | the humoral antibody response against the nonstructural protein ns1 and the structural protein vp2 of porcine parvovirus (ppv) was evaluated by immuno-peroxidase test (ipt) and enzyme linked immuno sorbent assay (elisa) using recombinant ppv antigens. the coding sequence for ns1 and vp2 was inserted into the baculovirus. autographa californica nuclear polyhedrosis virus (acnpv) genome resulting in two recombinant baculoviruses acnpv-ns1 and acnpv-vp2, respectively. sf9 cells (spodoptora frugidip ... | 1997 | 9050166 |
| virucidal treatment of blood protein products with uvc radiation. | the virus safety of blood derivatives continues to be of concern, especially with respect to nonenveloped and/or heat-stable viruses. previously, we demonstrated that treatment of whole plasma, ahf concentrate or fibrinogen with short wavelength ultraviolet light (uvc) results in the inactivation of > or = 10(6) infectious doses (id) of encephalomyocarditis virus (emcv), hepatitis a virus (hav) and porcine parvovirus (ppv), each of which is nonenveloped. protein recovery was enhanced greatly by ... | 1997 | 9077126 |
| antibody response of pigs to inactivated monovalent and bivalent vaccines for porcine parvovirus and pseudorabies virus. | groups of pigs vaccinated with an inactivated bivalent vaccine containing porcine parvovirus (ppv) and pseudorabies virus (prv) developed geometric mean titers (gmt) of humoral antibody for each of the viruses as high or slightly higher than those of other groups of pigs that were vaccinated with inactivated monovalent vaccines containing one or the other of the same viruses. an increase in gmt after challenge exposure of vaccinated pigs to live virus indicated that vaccination did not prevent v ... | 1980 | 6261613 |
| experimental infection of sheep by caprine arthritis-encephalitis virus and goats by progressive pneumonia virus. | the lentiviruses, caprine arthritis-encephalitis virus (caev) and progressive pneumonia virus (ppv) of sheep, cause major diseases in their respective hosts; however, the infectivity of these viruses for closely related species has not been determined. experiments were conducted to determine whether caev would infect sheep and whether ppv would infect goats. upon inoculation with caev, lambs developed a nonsuppurative arthritis and antibody to caev, and the virus was isolated up to 4 months late ... | 1983 | 6318613 |
| long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis. | the 5'-terminal 31 nucleotides of the 146-nucleotides-long 5' noncoding region of plum pox potyvirus (ppv) are highly conserved in all the members of the potyvirus genus. to map the sequences of the 5' noncoding region that are necessary in vivo for infectivity, we have constructed a nested set of substitution and deletion mutants. while we were not able to infect nicotiana clevelandii plants with full-length ppv transcripts bearing mutations in the 5'-terminal 35 nucleotides of the viral genome ... | 1997 | 9201225 |
| recombinant parvovirus-like particles as an antigen carrier: a novel nonreplicative exogenous antigen to elicit protective antiviral cytotoxic t cells. | to develop a strategy that promotes efficient antiviral immunity, hybrid virus-like particles (vlp) were prepared by self-assembly of the modified porcine parvovirus vp2 capsid protein carrying a cd8(+) t cell epitope from the lymphocytic choriomeningitis virus nucleoprotein. immunization of mice with these hybrid pseudoparticles, without adjuvant, induced strong cytotoxic t lymphocyte (ctl) responses against both peptide-coated- or virus-infected-target cells. this cd8(+) class i-restricted cyt ... | 1997 | 9207121 |
| specific detection of d- and m-isolates of plum pox virus by immunoenzymatic determination of pcr products. | molecular techniques based on the polymerase chain reaction (pcr) can provide rapid and sensitive diagnosis of plum pox virus (ppv), the causal agent of the devastating 'sharka' disease of stone fruit trees. the present study compared routine polymerase chain reaction (pcr) procedures against a new system, pcr-elisa (boehringer mannheim), which enables immunoenzymatic detection of pcr products. the results show that this hybridisation system ensures fast and more sensitive detection of ppv assoc ... | 1997 | 9300377 |
| comparison of two different methods for inactivation of viruses in serum. | in order to compare protocols for inactivation of viruses potentially present in biological specimens, three different model viruses were treated in bovine serum by two different inactivation methods: samples were subjected either to chemical inactivation with ethylenimine (el) at concentrations of 5 and 10 mm at 37 degrees c for periods up to 72 h or to electron-beam irradiation in frozen and liquid form with doses varying between 11 and 46 kgy. the chemical inactivation resulted in nonlinear t ... | 1997 | 9302195 |
| a congenital persistent infection of bovine virus diarrhoea virus in pigs: clinical, virological and immunological observations. | we report on a lifelong 'carrier' state of non-cytopathic bovine virus diarrhoea virus (bvdv) in an otherwise healthy pig. three out of 13 pigs of a litter congenitally infected with bvdv survived for more than 3 months. one pig was bvdv seropositive at 1 month, the second seroconverted between 6 and 8 months, and the third remained viraemic and bvdv-immunotolerant until slaughter at 26 months. the latter pig, a boar, excreted virus in oropharyngeal fluid, urine and semen. ejaculates, however, d ... | 1997 | 9323848 |
| validation of the heat treatment step used in the production of diaspirin crosslinked hemoglobin (dclhb) for viral inactivation--effect of crosslinking. | two experiments were performed to assess viral inactivation during the crosslinking and heat treatment steps of the dclhb manufacturing process. stroma free hemoglobin (sfhb) collected from a large scale manufacturing lot was tested in a 1:680 scaled down system in which the key parameters used in the manufacturing process were replicated. in the first study porcine parvovirus (ppv), a non-enveloped virus, was used to assess inactivation, while in the second study bovine viral diarrhea virus (bv ... | 1997 | 9352057 |
| non-suppurative myocarditis in piglets associated with porcine parvovirus infection. | the involvement of porcine parvovirus (ppv) in the aetiology of non-suppurative myocarditis in sucking piglets was investigated by a polymerase chain reaction (pcr), designed to assess the presence of viral genome in formalin-fixed paraffin wax-embedded tissue of diseased animals. myocardium and lung of stillborn piglets with a confirmed ppv infection were used to set up the pcr amplification method. subsequently, 20 myocardia with inflammatory lesions were examined in parallel with 20 myocardia ... | 1997 | 9352435 |
| isolation of porcine parvovirus (ppv) from swine herds affected by reproductive failure, and serologic evidence of infection in hungarian large swine herds. | | 1983 | 6331141 |
| simultaneous detection and typing of plum pox potyvirus (ppv) isolates by heminested-pcr and pcr-elisa. | two techniques for simultaneous detection and typing of plum pox potyvirus (ppv) isolates belonging to the d or m serotypes, heminested pcr (h-pcr) and pcr-elisa, have been developed. ten ppv isolates typed using ppv-d and ppv-m specific monoclonal antibodies by elisa-dasi were used to validate these two methods. the results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. when serial dilutions of infected plant extracts were assayed, h-pcr and ... | 1997 | 9389402 |
| plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations. | nicotiana benthamiana plants were transformed with a fragment of the plum pox potyvirus (ppv) genome that encodes the nuclear inclusion a (nia) and b (nib) proteins and the n-terminus of the capsid protein (nia-nib-cp). lines transformed with this ppv genomic fragment harboring mutations in the gdd replicase-motif were also obtained. plants of niadeltav lines that carry a gdd to vdd mutation in the ppv transgene, were immune to ppv infection. the resistance was highly specific, since it was only ... | 1998 | 9469941 |
| production of strain specific antibodies against a synthetic polypeptide corresponding to the n-terminal region of the plum pox potyvirus coat protein. | comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (ppv-swc) with the corresponding regions of several other ppv strains indicated that the main differences are in the n-terminal region. polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the n-terminal region of ppv-swc coat protein. they specifically detected ppv-swc in different immunochemical tests. | 1997 | 9504763 |
| transport of viruses through fetal membranes: an in vitro model of perinatal transmission. | a model system for perinatal transmission of viral infections was developed and transport of infectious virus particles through fetal membranes was investigated. viruses of different families known to cause serious intrauterine infections were selected, including relevant and model viruses: the dna-viruses hsv-1 and -2 as well as the animal herpes viruses bhv-1 and shv-1, the rna-virus bvdv as a model for hepatitis c virus, hiv-1 and -2, and ppv as a model for parvovirus b19. migration of infect ... | 1998 | 9557298 |
| specific oligonucleotide primers for the direct detection of plum pox potyvirus-cherry subgroup. | a specific polymerase chain reaction assay was developed for direct identification of the distinct subgroup of plum pox potyvirus (ppv) isolates from cherry trees (ppv-cherry, ppv-c) and its differentiation from other known subgroups of ppv. the specificity of the assay is based on using a pair of primers whose nucleotide sequences are located on the coat protein gene of ppv-sour cherry (soc) at regions of high nucleotide divergence between ppv-soc and other isolates of ppv. the technique will b ... | 1998 | 9562418 |
| the pattern of endemic parvovirus infection in four pig herds. | serological surveys were conducted on the gilts and adult sows in 4 herds endemically infected with porcine parvovirus. the study assessed the influence of the type of management of breeders on the spread of virus infection and the influence of endemic parvovirus infection on reproductive parameters of the herd. the practice of holding gilts and sows in groups did not reliably promote infection or maintain a 100% level of active immunity amongst adult sows in 2 of 3 group husbandry herds. in the ... | 1983 | 6626062 |
| detection of challenge virus in fetal tissues by nested pcr as a test of the potency of a porcine parvovirus vaccine. | to estimate the potency of a porcine parvovirus (ppv) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with ppv and the distribution of the virus was studied in the tissues of their 51 fetuses. virus detection was attempted using haemagglutination (ha) and immunofluorescence (if) assays, as well as by standard (single) and nested polymerase chain reactions (pcr). none of the detection methods yielded positive results when used to test for the presence of virus in s ... | 1998 | 9563172 |
| a recombinant virus-like particle system derived from parvovirus as an efficient antigen carrier to elicit a polarized th1 immune response without adjuvant. | hybrid virus-like particles (vlp) were prepared by self-assembly of the modified porcine parvovirus (ppv) vp2 capsid protein carrying a cd8+ or cd4+ t cell epitope. immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic t lymphocyte and t helper (th) responses against the reporter epitope. the th response was characterized by a th1 phenotype. we also analyzed in vitro the uptake mechanism of these parvovirus-like particles and the pro ... | 1998 | 9565380 |
| susceptibility of peach gf 305 seedlings and selected herbaceous plants to plum pox virus isolates from western slovakia. | the susceptibility of peach gf 305 seedlings and herbaceous plants to five plum pox virus (ppv) isolates from orchards of western slovakia was investigated. ppv was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach gf 305. the herbaceous plants were infected by mechanical inoculation. the transmission was analysed by symptomatology and double sandwich enzyme-linked immunosorbent assay (das-elisa). infected peaches developed leaf distortion, tissue cle ... | 1997 | 9607094 |
| increased litter size in gilts by intrauterine infusion of seminal and sperm antigens before breeding. | three experiments were conducted to evaluate the effect of exposure of the uterus to semen at least 3 wk before breeding on subsequent reproductive performance. in exp. 1, uterine exposure to semen was performed three times. at least 3 wk elapsed between each treatment. control gilts received saline infusion. all gilts were bred by artificial insemination using semen from the same boars used for semen treatment. at farrowing, significantly more (10.35 vs 8.39) pigs/litter were produced by semen- ... | 1983 | 6682857 |
| nicotiana benthamiana plants transformed with the plum pox virus helicase gene are resistant to virus infection. | nicotiana benthamiana domin. plants were transformed with the cytoplasmic inclusion protein (ci) gene of plum pox potyvirus (ppv) to investigate, whether this non-structural protein would be able to confer resistance. the ci protein is an rna helicase, which contains a conserved nucleotide binding motif (ntbm) and plays an important role in viral replication. two gene constructions were developed for plant transformation. the first contains the original coding sequence of the ci gene under the c ... | 1998 | 9617773 |
| mapping the antigenic structure of porcine parvovirus at the level of peptides. | the antigenic structure of the capsid proteins of porcine parvovirus (ppv) was investigated. a total of nine linear epitopes were identified by pepscan using porcine or rabbit anti-ppv antisera. no sites were identified with a panel of neutralising monoclonal antibodies (mabs). all epitopes were located in the region corresponding to the major capsid protein vp2. based on this information, and on analogy to other autonomous parvoviruses, 24 different peptides were synthesised, coupled to keyhole ... | 1998 | 9620208 |
| a comparative survey using the gel diffusion precipitin and haemagglutination-inhibition tests for porcine parvovirus antibody. | | 1982 | 6805458 |
| evaluation of a modified live-virus vaccine for the prevention of porcine parvovirus-induced reproductive disease in swine. | each of 5 gilts was vaccinated im with modified live-virus (mlv) vaccine for porcine parvovirus (ppv), and 5 gilts were used as nonvaccinated controls. vaccinated gilts developed hemagglutination-inhibiting (hi) antibodies to ppv (titer of 320 to 1,280) by 2 weeks after vaccination. all gilts wee bred, and at about 40 days of gestation their immunity was challenged by intranasal and oral administration of a virulent strain of ppv. gilts were killed at about 84 days of gestation and their litters ... | 1980 | 7212434 |
| reproductive performance of gilts exposed to porcine parvovirus at 56 or 70 days of gestation. | a total of 18 pregnant gilts, which were free of antibody for porcine parvovirus (ppv), were exposed oronasally to ppv on either the 56th day (9 gilts) or 70th day (9 gilts) of the gestation to determine whether infection at these times would affect their reproductive performance. the gilts were either necropsied late in gestation or allowed to farrow, and their fetuses and pigs were tested for evidence of infection. gilts remained clinically healthy throughout the experiment, and none farrowed ... | 1981 | 7340578 |
| strain variability of plum pox virus isolates from western slovakia. | leaf tissues of stone fruit trees (plum, apricot, peach and myrobalan) carrying symptoms of plum pox virus (ppv) infection and of peach gf 305 seedlings and nicotiana benthamiana infected experimentally with ppv were assayed for ppv by polymerase chain reaction (pcr). the expected 243 bp pcr products were subjected to restriction fragment length polymorphism (rflp) analysis with restriction endonucleases alui and rsai. all of the pcr products contained the alui site. the rsai restriction profile ... | 1998 | 9770072 |
| production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of mediterranean isolates. | monoclonal antibodies (mabs) specific to plum pox virus (ppv) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (cp). the characterized mabs could be used in elisa to differentiate several mediterranean ppv isolates differing in their geographical origin and cp size. at least seven antigenic sites could be established based on the recognition pattern and ... | 1994 | 7526822 |
| determination of adequate moisture content for efficient dry-heat viral inactivation in lyophilized factor viii by loss on drying and by near infrared spectroscopy. | a requirement for a minimal threshold level of moisture in order for efficient virus inactivation to occur during dry heat treatment of freeze-dried coagulation factor concentrates is described. techniques used to determine moisture content during heating were loss on drying and karl fischer. the loss on drying was suspected to have occasional errors as a result of sample preparation being influenced by interference from atmospheric moisture. therefore, a non-invasive, non-destructive method for ... | 1998 | 9811517 |
| inactivation of viruses by beta-propiolactone in human cryo poor plasma and igg concentrates. | virus inactivation by cold treatment with beta-propiolactone (bpl) was investigated in human cryo poor plasma and purified igg concentrates spiked with relevant human viruses or appropriate animal model viruses. the samples were treated with 0.1 or 0.25% bpl for 300 or 480 min, respectively. residual infectivity was determined by standard microtitration assays on tissue culture cells. the inactivation of all viruses tested was more effective in igg than in plasma. igg: r1=4-5.5 log10 for vesicul ... | 1998 | 9811521 |
| serosurvey of selected viral and bacterial diseases in wild swine from oklahoma. | blood samples collected from 120 wild swine (sus scrofa) in thirteen oklahoma (usa) counties during 1996 were tested for antibodies against six viral and two bacterial diseases. no antibodies to swine brucellosis, pseudorabies, transmissible gastroenteritis, and vesicular stomatitis were detected. antibody titers to one or more leptospiral serovars were found in 44% of the samples, the two most frequent serovars being leptospira interrogans serovars bratislava (29%) and pomona (27%). antibody ag ... | 1998 | 9813859 |
| prenatal diagnosis of congenital cytomegalovirus infection. | we report here the results of a study on the prenatal diagnosis of congenital cytomegalovirus (cmv) infection. the study was carried out by both pcr and virus isolation from amniotic fluid (af) for 82 pregnant women at risk of transmitting cmv for the detection of (i) seroconversion to cmv immunoglobulin g (igg) positivity during the first trimester of pregnancy, (ii) symptomatic cmv infection in the mother during the first trimester of pregnancy or intrauterine growth retardation detected by ul ... | 1998 | 9817869 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| development of a pcr-based method coupled with a microplate colorimetric assay for the detection of porcine parvovirus and application to diagnosis in piglet tissues and human plasma. | a new method for porcine parvovirus (ppv) diagnosis was developed. the method is based on polymerase chain reaction (pcr) amplification followed by hybridization and colorimetric detection of pcr products in microwell plates. a highly specific and sensitive amplification step was ensured by primers carefully selected in the vp2 structural gene and optimized pcr conditions. uracyl-dna-glycosylase (udg) in combination with dutp was used to avoid false-positive results, and 100 copies of internal c ... | 1998 | 9843658 |
| validation of the heat treatment step used in the production of diaspirin crosslinked hemoglobin (dclhb) for viral inactivation. | a series of experiments was performed to assess the ability of the heat treatment step used in the manufacture of diaspirin crosslinked hemoglobin (dclhb) to inactivate viruses. in-process solutions (reaction mixtures after the crosslinking process) from six different manufacturing lots were used as test media in a 1:680 scaled down system in which the key process parameters used in the large scale production were duplicated. the inactivation of five different viruses (bovine viral diarrhea viru ... | 1998 | 9844723 |
| ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus. | antisera to the bacterially expressed nonstructural proteins (nsp) hc-pro, ci, nia, and nib and the coat protein (cp) of plum pox potyvirus (ppv) were used for analysing the composition of virus-induced cytoplasmic and nuclear inclusions by electron microscopy. the antisera reacted with nsp and cp of ppv on immunogold-labelled ultrathin sections. antiserum to cp reacted with virions of seven out of 18 other potyviruses. cp was distributed throughout the cytoplasm of infected cells. antisera to p ... | 1998 | 9856098 |
| nucleotide sequence of the putative replicase gene of the sour cherry strain of plum pox potyvirus. | the complete nucleotide sequence of the nib coding region of the sour cherry strain of plum pox potyvirus (ppv-soc) has been determined. it consists of 1554 nucleotides and encodes a putative replicase protein of 518 amino acids. sequence identity scores between nib of ppv-soc and other isolates of ppv are significantly low (c. 78%). many of the nucleotide substitutions, however, are silent. ppv-soc differs from isolates of ppv-d, ppv-m and ppv-e1 amar at multiple amino acid positions that are c ... | 1998 | 9856106 |
| rna helicase activity of the plum pox potyvirus ci protein expressed in escherichia coli. mapping of an rna binding domain. | the plum pox potyvirus (ppv) cylindrical inclusion (ci) protein fused to the maltose binding protein (mbp) has been synthesized in escherichia coli and purified by affinity chromatography in amylose resin. in the absence of any other viral factors, the fusion product had ntpase, rna binding and rna helicase activities. these in vitro activities were not affected by removal of the last 103 amino acids of the ci protein. however, other deletions in the c-terminal part of the protein, although leav ... | 1995 | 7538661 |
| transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component. | two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ... | 1995 | 7561767 |
| expression of the plum pox coat protein gene in transgenic nicotiana tabacum plants. | plant expression vector pbi 121 containing the gene encoding coat protein of plum pox virus of the skierniewice isolate (cp ppv-s) was prepared (clone pcm1). the construct was used for transformation of nicotiana tabacum plants using an agrobacterium tumefaciens based system. about 82% of kanamycin resistant plant lines contained a transgene (the sequence of cp ppv-s) but only 81% of them actively expressed the ppv-s coat protein gene as measured by rt-pcr. | 1995 | 7653168 |
| reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. | neonatal gnotobiotic piglets were inoculated with tissue homogenates and low- and high-passage cell culture material to determine if the lesions of the newly described porcine postweaning multisystemic wasting syndrome (pmws) could be reproduced. for this, 17 3-day-old gnotobiotic piglets were inoculated intranasally with pelleted chloroform-treated, filtered extracts from cell cultures, filter-sterilized homogenates of lymphoid tissue from pmws-affected piglets, or control materials. piglets we ... | 1999 | 9925205 |
| mitotic stability of infection-induced resistance to plum pox potyvirus associated with transgene silencing and dna methylation. | plum pox potyvirus (ppv) infection of transgenic nicotiana benthamiana plants that expressed the ppv nib rna replicase carrying a gly to val mutation at the gdd motif (nibv lines) induced a phenotype of virus resistance and transgene silencing, which was not transmissible to the progeny after self-fertilization (h. s. guo and j. a. garcía, mol. plant-microbe interact. 10:160-170, 1997). here, we demonstrate that the induced resistance of nibv plants is mitotically stable after plant propagation ... | 1999 | 9926412 |
| reproductive tract infections in primary healthcare, family planning, and dermatovenereology clinics: evaluation of syndromic management in morocco. | to determine where and with what symptoms women seek care for reproductive tract infections (rti) in morocco and to guide allocation of resources for training and treatment for rtis. | 1998 | 10023358 |
| nucleotide sequence of the coat protein gene of the skierniewice isolate of plum pox virus (ppv). | the coat protein (cp) gene of the skierniewice isolate of plum pox virus (ppv-s) has been amplified using the reverse transcription--polymerase chain reaction (rt-pcr), cloned and sequenced. the nucleotide sequence of the gene and the deduced amino-acid sequence of ppv-s cp were compared with those of other ppv strains. the nucleotide sequence showed very high homology to most of the published sequences. the motif: asp-ala-gly (dag), important for the aphid transmissibility, was present in the a ... | 1994 | 7913278 |
| comparative sequence analysis of four complete primary structures of plum pox virus strains. | the complete nucleotide sequence of plum pox virus (ppv) strain sk 68 was determined from a series of overlapping cdna clones. the exact 5' terminus was determined by direct rna sequencing. the rna sequence was 9786 nucleotides in length, excluding a 3' terminal poly(a) sequence. the large open reading frame starts at nucleotide position 147 and is terminated at position 9568. comparison of cistrons from other plum pox virus strains with those predicted for the sk 68 strain indicated the same ge ... | 1993 | 8122394 |
| comparison of different methods of rna isolation for plum pox virus detection by reverse transcription-polymerase chain reaction. | the diagnosis of plum pox virus (ppv) is still considered one of the most important aspects of the "sharka" problem. in fact, different studies demonstrated an uneven distribution of the virus in infected trees due to a high variability in virus concentration. these aspects complicate the ppv diagnosis. to date, biological, serological and molecular assays have been successively developed in order to obtain sensitive and efficient ppv detection techniques. in particular, the polymerase chain rea ... | 1998 | 10073221 |
| investigation on the plum pox virus resistance in different apricot genotypes. | in our three-year investigation, 164 apricot trees of different old german varieties cultivated in the mansfelder land region were tested for the plum pox virus (ppv) resistance by double grafting in greenhouse conditions using an isolate of ppv d strain from our region. we selected 25 genotypes with quantitative resistance and two with immunity. the first results of field trials are comparable with those from greenhouse. with cvs. virosia and brevira, two local quantitatively resistant varietie ... | 1998 | 10073222 |
| characterization of plum pox virus isolates from slovakia. | plum pox virus (ppv) isolates from stone-fruit trees (plums, myrobalans, apricots and peaches) from orchards and gardens were characterized. to characterize their biological properties, several ppv isolates were transmitted by chip budding to gf 305 seedlings and mechanically to selected herbaceous test plants. the isolates differed in severity of infection, host range and symptomatology. a subgroup differentiation of 43 isolates from 22 localities of western and middle slovakia was accomplished ... | 1998 | 10073223 |
| aphid species--vectors of plum pox virus. | plum pox virus (ppv) is widely spread by natural vectors present in plum orchards. the efficiency of transmission is dependent on the frequency of the occurrence of vectors and the cultivar susceptibility to this pathogen. having in view that ppv has a wide range of annual and multiannual host plants and vectors, there is great concern for obtaining ppv-resistant cultivars. this report deals with the following vectors: hyalopterus pruni, brachycaudus cardui, brachycaudus helichrysi. myzus persic ... | 1998 | 10073225 |
| high resistance and control of biological risks in transgenic plants expressing modified plum pox virus coat protein. | transgenic plums transformed with the plum pox virus coat protein (ppv cp) gene displayed a resistance to the sharka disease (ravelonandro et al., 1997). however, the expression of ppv cp in transgenic plants may lead to complementation of deficient characteristic of an incoming potyvirus. indeed, an aphid-intransmissible strain of zucchini yellow mosaic virus (zymv-nat) could be transmitted when encapsidated by the engineered ppv cp (lecoq et al., 1993). to control such a risk, new ppv cp const ... | 1998 | 10073226 |
| mineral nutrition of plum trees as an important factor of protection against plum pox virus disease. | the experimental results obtained from a fruit garden at krajné pointed out a different content of macrobiogenic elements in the leaves of plum trees infected with plum pox virus (ppv) as compared to healthy ones. mineral nutrition of diseased trees was characteristic by lower relational values of topical levels of macrobiogenic elements especially those of n/p, n/k, (ca + mg)/k, and (n/p)/(n/k). | 1998 | 10073227 |
| preliminary report on the apparent breaking of resistance of a transgenic plum by chip bud inoculation of plum pox virus ppv-s. | five transgenic clones of prunus domestica l. containing plum pox virus (ppv) coat protein (cp) gene and one non-transformed control clone were challenged with ppv-s in the field. symptoms developed on c2, c3, c4, c6 and b70146 but not c5 trees inoculated by chip budding (cbi) (2/2, 2/2, 1/1, 2/2 and 2/2, positive/inoculated) in the first summer after inoculation. however, in the second year, symptoms appeared on cbi c5 trees. the presence of the virus in the plants was confirmed by enzyme-linke ... | 1998 | 10073228 |
| new results concerning the plum pox virus epidemiology and resistance of plum cultivars, hybrids and rootstocks. | our recent results on the plum pox virus (ppv) epidemiology show that ppv spreads very rapidly in plum tree plantations in the contaminated areas. a clearing of the ppv-infected trees reduces significantly the spread of the virus but does not eliminate the disease. some plum tree cultivars, hybrids and rootstocks (scoldus, alina, cristi, bn 1/8fl, bn 2gr. etc) showing field resistance could not be infected with ppv by natural way. however, they could be infected with ppv by artificial inoculatio ... | 1998 | 10073229 |
| production of a monoclonal antibody specific to the el amar strain of plum pox virus. | plum pox virus (ppv) isolates are grouped into three clusters differentiated by biological, serological, molecular and epidemiological characteristics: marcus (m), dideron (d) and cherry (c). the el amar (ea) isolate that does not fit any of the above groups is also known. monoclonal antibodies (mabs) that specifically recognize m, d, and c strains of ppv are already available. to complete the set of ppv strain-specific serological reagents, mabs against the ea isolate were raised by immunizing ... | 1998 | 10073230 |
| detection and serotyping of mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies. | plum pox virus (ppv) is a major threat to the expanding mediterranean stone fruit industry. in order to control the plum pox disease it is of utmost importance to detect early ppv foci and to identify the ppv isolates involved. a survey was therefore carried out in albania, cyprus, egypt, greece, italy and turkey by a double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa) with the following monoclonal antibodies (mabs): 5b (universal), 4dg5 (ppv-d-specific), al (ppv-m-specific), ... | 1998 | 10073231 |
| molecular variability of czech plum pox virus isolates. | seventy-two czech plum pox virus (ppv) isolates from different hosts were tested by double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa). in addition, the coat protein mobility and the rsai restriction fragment length polymorphism (rflp) pattern of reverse transcription-polymerase chain reaction-(rt-pcr) amplified coat protein (cp) gene fragment of the isolates were analysed. both ppv-d and ppv-m serotypes were found in the czech republic. the results obtained by these differen ... | 1998 | 10073232 |
| testing of plum germplasm for sensitivity to plum pox. | a long-term orchard experiment with a broad assortment of plum cultivars aimed to screen their sensitivity to plum pox virus (ppv) was established in 1991. for this purpose, 207 cultivars to be artificially infected with ppv at a permanent site were chosen. the serotype m of ppv from a tree of cv. domestic prune, which had not been contaminated by other viruses, was used as a source of the infection. three buds infected with ppv were budded on 1-year-old trees. in the course of experiment the fo ... | 1998 | 10073233 |
| detection of plum pox virus in apricot seeds. | twelve different apricot selection trees from a germplasm collection naturally infected with plum pox virus (ppv) were chosen to investigate the role of seeds in the epidemiology of this dangerous pathogen. all the considered plants showed typical symptoms on leaves and fruits and were positive in enzyme-linked immunosorbent assay (elisa). the virus was characterized by immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) followed by restriction fragment length polymorphism ... | 1998 | 10073234 |
| relative concentration of plum pox virus in leaves and flowers of some prunus species and cultivars. | the relative concentration of plum pox virus (ppv) in leaves and flowers of plum, damson, myrobalan, blackthorn, apricot and peach trees was determined by enzyme-linked immunosorbent assay (elisa) and expressed as the lowest dilution with positive reaction. significant differences in relative ppv concentration were found in leaves among individual prunus species naturally or artificially infected with the virus. the highest relative ppv concentration was found in blackthorns (7.81 x 10(-4)), com ... | 1998 | 10073235 |
| restriction fragment length polymorphism differentiation of plum pox virus isolates. | reverse transcription-polymerase chain reaction (rt-pcr) technique and restriction fragment length polymorphism (rflp) analysis were used to analyse six isolates of plum pox virus (ppv). whole coat protein (cp) gene was amplified in four isolates using the unipoty-polyt primer pari. ppv-d was identified by rflp analysis using sfui and drai enzymes in two of the isolates. two isolates of ppv-m strain yielded rt-pcr products which could not be digested by the two enzymes. other isolates were subje ... | 1998 | 10073236 |