| [the seasonal appearance of swim bladder inflammation in the course of sphaerospora renicola infection in carp]. | in carp during 1st year the course of dynamics of the 3 known stages of sphaerospora renicola showed an increase of the kidney infection in winter and spring (with inhibited sporulation). the winter/spring-peak was accompanied by an intensive blood-phase (c-stages). after august of the 1st summer the swimbladder-phase (k-stages) was not observed, although sbi occurred between january and april. under warm water condition this infection was discharged after september/october, the sbi never occurr ... | 1990 | 2085211 |
| [a novel group of nucleolar organizer region associating proteins]. | with the scleroderma autoantinucleolar antiserum which was discribed in our previous paper, we identified a novel group of nucleolar organizer associating proteins, anop. by means of immunoblotting, it was found that anop mainly consists of three polypeptides with molecular weights of 65, 76, and 78 kd respectively. immunoelectron microscopy demonstrated that anop is localized in the shells of dense fibril components surrounding fibrillar centers of nucleolus. moreover, indirect immunofluorescen ... | 1990 | 2091420 |
| rna-binding protein-related sequence in a malaria antigen, clustered-asparagine-rich protein. | members of the rna-binding protein superfamily contain rna binding domains of about 90 amino acids with a highly conserved motif 'gfgf'. using the conserved motif with some variations g-(f/y)-(g/a)-(f/y)-(v/i)-x-(f/y) as a probe, we screened protein sequences carrying identical amino acids in an nbrf-protein database. it has been found that the c-terminal portion of clustered asparagine-rich protein (carp), a malaria antigen from plasmodium falciparum, shows an unexpected sequence similarity wit ... | 1990 | 2105236 |
| growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. | an established fish cell line, epithelioma papillosum cyprini (epc), originating from the carp (cyprinus carpio) was found to be susceptible to the penaeid shrimp virus, infectious hypodermal and hematopoietic necrosis virus (ihhnv). this economically important pathogen of penaeid shrimp raised in mariculture has been previously limited to growth only in young post-larval shrimps. this is, to our knowledge, the first reported successful growth of the penaeid virus in a fish cell line; it promise ... | 1990 | 2117021 |
| intestinal trematodes of humans in korea: metagonimus, heterophyids and echinostomes. | ten species of the trematode family heterophyidae (metagonimus yokogawai, m. takahashii, heterophyes nocens, h. heterophyes, h. dispar, heterophyopsis continua, pygidiopsis summa, stellantchasmus falcatus, centrocestus armatus and stictodora fuscatum), and 3 species of the family echinostomatidae (echinostoma hortense, e. cinetorchis and echinochasmus japonicus) have been found to infect humans in korea. biological and epidemiological studies on the above species have shown that metagonimus, het ... | 1990 | 2133416 |
| isolation and characterization of beta-actin gene of carp (cyprinus carpio). | a beta-actin gene of carp (cyprinus carpio) was isolated from a genomic embl3 library. the nucleotide sequence of the gene indicates six exons spanning 3.6 kb. southern blot hybridization of restriction endonuclease digests of carp genomic dna indicate that there are two copies of the beta-actin isotype and several other species of actin genes. the transcriptional start site is 85 bp and 24 bp downstream respectively from consensus ccaat and tata promoter elements. the organization of the carp b ... | 1990 | 2134183 |
| [the biology and pathogenicity of the carp parasite thelohanellus nikolskii (myxosporidia)]. | the myxosporidian thelohanellus nikolskii has been recorded in young of carp in fish-breeding ponds of moldavia since 1982. sporogenesis takes place from june to august. single cysts with mature spores are found in winter and spring. the distribution of cysts in the host's population is of overdispersed character. haematological investigation has revealed the threshold level of the invasion when there are observed changes in the blood of infected fishes (8 to 10 cysts per 1 g of fish weight). th ... | 1990 | 2147986 |
| purification and subcellular localization of zn-dependent acid p-nitrophenylphosphatase in frog liver and comparison with other vertebrates. | zn2(+)-dependent acid p-nitrophenylphosphatase (zn-acpase) from liver of rana esculenta was purified to homogeneity. the purified enzyme moves as a single electrophoretic band at ph 8.3 in 7.5% acrylamide and was coincident with the enzyme activity. the enzyme has a molecular weight of 102,000 +/- 5,000d and is a dimer with two apparently similar polypeptide chains of 48,000 +/- 3,000d as determined by sodium dodecylsulfate gel electrophoresis. zn-acpase from frog liver requires zn2+ ions for ca ... | 1990 | 2161444 |
| angiotensin-converting enzyme distribution and hypoxia response in mammal, bird, and fish. | angiotensin i-converting enzyme activity was measured in homogenates of guinea pig (cavia porcellus), chicken (gallus domesticus), and carp (cyprinus carpio) organs. the highest activity was found in guinea pig lung and chicken kidney. in carp the highest activity was found in heart and spleen, although gill arch also showed a high activity. acute hypoxia decreased the angiotensin i-converting enzyme activity in guinea pig lung and carp gill arch, but the changes in chicken lung and kidney were ... | 1990 | 2167864 |
| herpesviruses detected in papillomatous skin growths of koi carp (cyprinus carpio). | herpesviruses were found associated with epidermal hyperplasia of koi carp (cyprinus carpio) in northern california (usa). papillomas were found principally on the caudal regions of the fish including the fins. the growths occurred most commonly in the fall and winter among populations of captive carp. infected epidermal cells were characterized by greatly enlarged nuclei depleted of chromatin but with thickened nuclear membranes. numerous virions were detected in infected cells. herpesvirus nuc ... | 1990 | 2174469 |
| histamine in the retina: recent progress and perspectives. | this article presents some new findings, and shortly surveys recently published data, on histamine (ha) in the retina of several vertebrates (carp, hen, rabbit, rat, guinea pig, cow, man). analysis of various parameters (ha level, activity of histidine decarboxylase and ha-methyltransferase, release and uptake of ha, 3h-mepyramine binding, effect of ha on camp accumulation, diurnal variations) in vertebrate retina and brain shows that histaminergic systems in these sites are comparable, although ... | 1990 | 2196761 |
| immunological and ultrastructural characterization of true histiocytic lymphoma in the northern pike, esox lucius l. | an epidemic of a malignant neoplasm occurs in northern pike, esox lucius l., from the aland islands of finland. the neoplasm is morphologically similar to other pike hemic tumors reported in other areas of the world. pike normal tissues showed evolutionary conservation with the mammalian intermediate filament proteins cytokeratin, desmin, vimentin, neurofilament protein, and glial fibrillary acidic protein; tumor cells are positive for vimentin, suggesting that the neoplasm is of mesenchymal ori ... | 1990 | 2201440 |
| close tip-to-tip contacts between dendrites of transient amacrine cells in carp retina. | in isolated retinas of the carp (cyprinus carpio) placed receptor side up in a plastic chamber, a subclass of amacrine cells, generating a fast on-off transient response to spot and annular light stimuli, were intracellularly recorded and injected with a fluorescent dye, lucifer yellow (ly). after brief fixation of the same preparations in aldehyde solution, the retinas were wholemounted vitreous side up in a tissue chamber. under a fluorescence microscope, one ly-injected cell and several dye-c ... | 1990 | 2216051 |
| growth, morphology and division of flagellates of the genus trypanoplasma (protozoa, kinetoplastida) in vitro. | nine strains of trypanoplasms were grown in axenic culture. cultures of trypanoplasma borreli laveran and mesnil, 1901 from fish hosts blicca bjoerkna, cyprinus carpio, scardinius erythrophthalmus and tinca tinca and of t. guerneyorum minchin, 1909 from esox lucius and trypanoplasma spp. from the leech piscicola geometra were maintained in biphasic blood-agar medium snb-9 supplemented with vitamins and antibiotics. in culture, the flagellates transformed into smaller, elongated stages with a lit ... | 1990 | 2217113 |
| structure of oncomodulin refined at 1.85 a resolution. an example of extensive molecular aggregation via ca2+. | the crystal structure of oncomodulin, a 12,000 mr protein isolated from rat tumours, has been determined by molecular replacement using the carp parvalbumin structure as a starting model. refinement was performed by cycles of molecular fitting and restrained least-squares, using area-detector intensity data to 1.85 a resolution. for the 5770 reflections in the range 6.0 to 1.85 a, which were used in the refinement, the crystallographic r-factor is 0.166. the refined model includes residues 2 to ... | 1990 | 2231727 |
| the effect of temperature on catalytic function of glyceraldehyde-3-phosphate dehydrogenase from muscle of pig and carp cyprinus carpio. | 1. the temperature dependence of the kinetics of glyceraldehyde-3-phosphate dehydrogenase from white muscle of carp and skeletal muscle of pig was examined. 2. the km values of carp muscle enzyme were stable over the temperature range 5-35 degrees c, but increased for pig muscle enzyme with increasing temperature. 3. the arrhenius plot for pig muscle enzyme is linear but non-linear for carp muscle enzyme. 4. the differences indicate that glyceraldehyde-3-phosphate dehydrogenase from white carp m ... | 1990 | 2253480 |
| comparison of the gamma-crystallins isolated from eye lenses of shark and carp. unique secondary and tertiary structure of shark gamma-crystallin. | gamma-crystallin isolated from the shark of cartilaginous fishes was compared with the cognate gamma-crystallin from the carp of bony fishes. distinct differences in amino acid compositions, primary, secondary and tertiary structures were found. the most salient features of shark gamma-crystallin lie in the fact that this crystallin possessed a significant alpha-helical structure in the peptide backbone as revealed by circular dichroism study, in contrast to those orthologous gamma-crystallins f ... | 1990 | 2261977 |
| allergy to different fish species in cod-allergic children: in vivo and in vitro studies. | the presence of a positive clinical history and skin test (st) results for 17 fish species (anchovy, bass, carp, dogfish, eel, gilthead, mackerel, mullet, perch, red mullet, salmon, sardine, sole, tench, toothed gilthead, trout, and tuna) were investigated in 20 children with cod-positive clinical history, st, and rast, and in 40 children positive to one or more foods different from cod (cow's milk, chicken egg white, peanut, and tomato). in cod-positive children, positive clinical history (60%) ... | 1990 | 2262645 |
| immunological evidence that the neural adhesion molecule l1 is expressed in fish brain and optic nerve: possible association with optic nerve regeneration. | in the mammalian peripheral nervous system (pns), expression of the neural adhesion molecule l1 on schwann cells and neurons has been correlated with axonal growth during development and regeneration. the present study was undertaken to examine whether a similar correlation exists between a lesion-induced increase of l1 expression and regenerative capacity in the central nervous system (cns). the fish optic nerve was used as a model for a successfully regenerating region of the cns. immunochemic ... | 1990 | 2265355 |
| antigen-capture elisa for viral haemorrhagic septicaemia virus serotype i. | an antigen-capture elisa for viral haemorrhagic septicaemia virus serotype i (vhsv i) was developed. the assay employs two monoclonal antibodies (mab) directed against distinct epitopes of the viral envelope glycoprotein (gp). the antigen bound by the capture mab (a17) was detected by addition of a second mab (l7) conjugated to horseradish peroxidase, followed by addition of the enzyme substrate. the technique is highly sensitive, enabling detection of the virus at a protein concentration as low ... | 1990 | 2266147 |
| [ultrastructure of the renal corpuscle of fresh water fishes]. | by means of light (semithin slices) and electron microscopes structural organization of the corpuscle and glomerulus of the capsule has been studied in freshwater fishes--carp (cyprinus carpio) and crucian carp (carassius carassius). the diameter of their glomeruli are in the carp from 80.8 +/- 1.4 mcm up to 59.8 +/- 2.4 mcm and in the crucian carp--from 86.4 +/- 1.8 mcm up to 69.25 +/- 0.2 mcm. between these dimensions there are significant differences. components of the external wall of the ca ... | 1990 | 2268195 |
| the complete amino acid sequence of prolactin from the sea turtle (chelonia mydas). | the complete amino acid sequence of prolactin (prl) from a reptile, the sea turtle (chelonia mydas), was determined for the first time. sequence analysis was performed on fragments obtained from cleavage of intact and performic acid-oxidized hormone with lysyl endopeptidase, staphylococcus aureus protease, and o-iodosobenzoic acid employing manual edman degradation. the sea turtle prl consists of 198 amino acid residues with three disulfide linkages formed between residues 4-11, 58-173, and 190- ... | 1990 | 2289679 |
| population dynamics and interrelationships of some dactylogyrus and gyrodactylus species on cyprinus carpio. | during 1986/87, 922 carps have been examined for ectoparasitic helminths. altogether 6 species of gyrodactylus and 4 species of dactylogyrus were found. the most frequent parasite on the gills was d. extensus; it shows a great tolerance to the water temperature with two peaks of infestation between 2 and 9 degrees c. additionally, relationships between the seasonal dynamics of the invasion cycle of d. extensus and d. anchoratus were recognized. in addition to dactylogyrus 2 species of gyrodactyl ... | 1990 | 2291498 |
| the role of tubificid oligochaetes in the transmission of goussia carpelli. | tubifex tubifex and limnodrilus hoffmeisteri, fed intestinal tissue of carp containing oocysts of goussia carpelli, produced infections in laboratory-reared carp. sporocysts ingested by the tubificids released the sporozoites that were found to be motile in the intestinal contents and incorporated in intestinal cells of the oligochaetes. tubificids remained infective for carp at least up to 57 days postexposure (pe). tubificids were also able to transmit g. carpelli to carp that had recovered fr ... | 1990 | 2299515 |
| inhibition of glutathione reductase by oncomodulin. | evidence for a specific interaction between oncomodulin and glutathione reductase is presented. glutathione reductase (ec 1.6.4.2) isolated from either the bovine intestinal mucosa or the rat liver was bound in a ca2(+)-dependent manner to oncomodulin which was covalently attached to sepharose. in addition, glutathione reductase was able to catalyze the reduction of the disulfide-linked dimer of oncomodulin. the interaction of these proteins could also be indirectly demonstrated by monitoring gl ... | 1990 | 2306116 |
| in vivo effect of the organophosphorus insecticide trichlorphon on the immune response of carp (cyprinus carpio). i. effect of contamination on antibody production in relation to residue level in organs. | the organophosphorus insecticide trichlorphon was tested at medium (10 and 20 ppm) and high concentrations (20,000 ppm) on antibody production against yersinia ruckeri vaccine. no statistically significant effect was observed on humoral response or on hematocrit. contamination of organs was analyzed by acetylcholinesterase activity. at 20,000 ppm only liver and brain were highly contaminated; spleen and lymphocytes were not significantly contaminated. at 20 ppm no residue was found in organs. | 1990 | 2311566 |
| in vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (cyprinus carpio). ii. effect of high doses of trichlorphon on nonspecific immune response. | the effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (cyprinus carpio) was studied. the effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. the effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by pseudomonas alcaligenes and aeromonas punctata was also examined. leucocyte number, phagocytic ability of neutrophils, ... | 1990 | 2311567 |
| light and electron microscopic observations on the swimbladder stages of sphaerospora renicola, a parasite of carp (cyprinus carpio). | the developmental cycle of stages of sphaerospora renicola, proliferating in the walls of the swimbladder in carp fry and responsible for swimbladder inflammation (= sbi stages) is described. the initial stage, a primary cell containing two secondary cells, grows in size while the secondary cells divide and produce two tertiary cells each by endogenous division. after a supposed disintegration of the grown primary cell, the cell triplets are released to repeat the cycle. the cell divisions are a ... | 1990 | 2315282 |
| mitogenic activity from trout embryos. | an extract of 21-day rainbow trout embryos stimulated growth of several piscine cell lines in the absence of added serum. established lines from trout (rtg-2 and ste-137), salmon (chse-214), carp (epc), and goldfish (car) and early-passage cells initiated from trout embryos grew in serum-free medium containing the embryo extract. in addition the extract was sufficient for maintaining long-term cultures of chse-214 cells for several months through a minimum of 20 passages (approximately 50 popula ... | 1990 | 2333296 |
| gulonolactone oxidase is missing in teleost fish. the direct spectrophotometric assay. | data in the literature imply that some fish species evolved with the capacity to synthesize ascorbic acid. gulonolactone oxidase activity has been reported in kidney and/or liver tissues. however, it is shown here that this microsomal enzyme activity is missing in common carp hepatopancreas and kidney, whereas high activity was confirmed in pigeon kidney, rat liver, bovine liver and amphibian (xenopus) kidney tissues. a new assay using either the whole tissue homogenate or microsomes solubilized ... | 1990 | 2340104 |
| functional analysis of elements affecting expression of the beta-actin gene of carp. | regulatory regions of the beta-actin gene of the common carp (cyprinus carpio) have been examined by linking upstream, 5'-flanking sequences and regions of the first intron to a bacterial chloramphenicol acetyltransferase (cat) reporter gene. by analysis of the mrna products and encoded cat activity, we have identified four putative regions that influence expression: (i) a negative regulatory region 2,300 to 1,100 base pairs (bp) ahead of the gene; (ii) a proximal promoter element, containing th ... | 1990 | 2355913 |
| effect of levamisole on the lymphocyte and macrophage activity in carp (cyprinus carpio). | levamisole, an anthelminthic drug which is often used in cattle, is known for its immunomodulatory effect in several species. in the present work we investigated the in vitro effects of different concentrations of levamisole on lymphocyte proliferation with mitogen in carp. at low concentrations, ie 12.5 to 0.1 micrograms/ml, levamisole stimulated proliferation with phytohemagglutinin p (pha-p) and con a, had no effect at medium concentrations and acted as a suppressor at high concentrations, ie ... | 1990 | 2360777 |
| effect of beta-naphthoflavone and mcpa on liver and kidney drug-metabolizing enzymes from the carp, cyprinus carpio. | the effects of beta-naphthoflavone (beta-nf) and a chlorophenoxyacetic acid herbicide (mcpa) on hepatic and renal monooxygenase activities and conjugating enzymes from immature carp (cyprinus carpio) were studied. beta-nf increased hepatic monooxygenase activities but the patterns of differential induction generally obtained in rat liver microsomes with two series of homologous substrates, alkoxycoumarins and alkylresorufins, were not found to be similar in carp liver microsomes. on the other ha ... | 1990 | 2364910 |
| lymphomyeloid cells, susceptibility to erythrodermatitis of carp and bacterial antigens. | pronounced changes occur in the kidneys carp injected with extracellular product (ecp), lipopolysaccharide (lps) of a. salmonicida subsp. salmonicida, freund's complete adjuvant (fca). ecp caused 50-70% decrease in macrophages and neutrophils, whereas blasts and lymphocyte-like cells increased fca-elicited increased percentages of blasts and a left shift of neutrophils after lps injection. after both ecp and fca injections, the density of major leucocyte types and red blood cells (rbc) in the ki ... | 1990 | 2369967 |
| [clinico-parasitological research in a mixed focus of clonorchiasis and intestinal nematodiasis in hanamnin province (the socialist republic of vietnam)]. | the authors described the clonorchiasis focus in the vietnam province at the seaside delta of the red river where the aforementioned invasion was accompanied with multiple invasions with intestinal nematodes. clonorchiasis infestation rate was 28.4% ascaridiasis, 50.5%, trichocephaliasis, 21.3% ancylostomiasis, 7.0%. clonorchiasis invasion was the result of consumed uncooked carp fish in the salad. it was revealed that the fish was reared in the ponds near the settlements and was fed untreated h ... | 1990 | 2377136 |
| a comparative study of the unfolding thermodynamics of vertebrate metmyoglobins. | differential scanning microcalorimetry (dsc) of horse, rat, opossum, raccoon, carp, and armadillo metmyoglobins at alkaline ph gave data that fit the two-state unfolding model well. monte carlo studies were used to assess the impact of truncating dsc scans on the reliability of the calculated results when aggregation exotherms overlapped the unfolding endotherm at the high-temperature end of the scan. the dsc estimates for the conformational free energy at ph 8 and 298 k are compared to earlier ... | 1990 | 2378866 |
| gene transfer, expression and inheritance of prsv-rainbow trout-gh cdna in the common carp, cyprinus carpio (linnaeus). | a recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to rainbow trout (salmo gairdneri) growth hormone (gh) cdna was microinjected into fertilized carp eggs. genomic dna extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and southern blot hybridization, using the rsv-ltr and/or the gh cdna sequences as probes. out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain prsv-rtg ... | 1990 | 2393581 |
| isolation of carp genes encoding major histocompatibility complex antigens. | in the evolution of the adaptive immune system unique to vertebrates, teleost fish occupy the critical position. this is the most primitive class of lower vertebrates in which the capacity for acute allograft rejections can be demonstrated, thus suggesting the presence of major histocompatibility complex (mhc) antigens and, therefore, t cells. here, we report the identification of two putative mhc-antigen-encoding sequences in the carp cyprinus carpio. one, identified as tlai alpha-1, had reason ... | 1990 | 2395879 |
| distribution of gaba-like immunoreactivity in myenteric plexus of carp, frog and chicken. | the distribution of gaba-like immunoreactivity was studied by means of indirect immunocytochemical methods in some lower vertebrate species (carp, frog, chicken). an immunoreactive network was revealed in the myenteric plexus of the alimentary canal of carp. gaba-positive nerve cells were attached closely to the fibres in the stomach. in other gut regions immunostained neurons were less frequent. immunoreactive fibres often formed baskets on the surfaces of immunonegative neurons along the whole ... | 1990 | 2401638 |
| [the effect of silver carp oil on the blood pressure and lipids of spontaneously hypertensive rats]. | | 1990 | 2402274 |
| effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio. | the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ... | 1986 | 2429432 |
| preparation and some properties of carp heavy meromyosin. | trypsin fragments carp myosin heavy chain into components of lower molecular weight. these changes are concomitant with the loss of ca2+-atpase and a weakening in actin-myosin interaction. as heavy meromyosin (hmm) prepared from myofibrils is more homogeneous, the above changes are due to overfragmentation of heavy chain mass. amino-acid composition of carp and rabbit hmm's are similar, but differences exist in response to dtnb and incorporation. | 1986 | 2434053 |
| immune response of carps (cyprinus carpio l.) against dextran. | in carps no antibodies could be evoked by immunization with soluble high molecular weight dextran using doses of 1-5000 mg dextran per kg body weight. on the other hand, an immune response against dextran could be elicited by immunization with a vaccine of leuconostoc mesenteroides b512 as well as a dextran-bsa-conjugate. these carp anti-dextran antibodies showed precipitating and agglutinating activities with specificity against alpha linked dextran determined by the almost complete inhibition ... | 1987 | 2439389 |
| mechanism of the interaction between myelin basic protein and the myelin membrane; the role of arginine methylation. | the addition of solutions of bovine myelin basic protein to suspensions of unilamellar vesicles prepared from whole myelin suspensions results in the rapid equilibrium association of the vesicles into dimers, followed by time-dependent aggregation reactions. other cationic proteins also induce the dimerization of the vesicles and equilibrium constants for dimer formation are obtained for bovine myelin basic protein, lysozyme, polyhistidine and myelin basic protein from carp, which differs from t ... | 1987 | 2440426 |
| effect of gamma-hexachlorocyclohexane (lindane) on carp (cyprinus carpio). i. effect of chronic intoxication on humoral immunity in relation to tissue pollutant levels. | lindane, the gamma-isomer of hexachlorocyclohexane, one of the most widely used insecticides, was incorporated into carp food for 109 days. the effects of 10, 100, or 1000 ppm of lindane in food on hematocrits and on antibody production against yersinia ruckeri bacterin in sera and mucus were followed for 79 days. no effect of lindane on these parameters and on spleen weight was observed. lymphoid organs, spleen and kidney, were as highly contaminated at the beginning of the inoculation (day 30) ... | 1987 | 2440660 |
| oxygen bonding in human hemoglobin and its isolated subunits: a xanes study. | the x-ray absorption near edge structure (xanes) spectra of the human adult and foetal hemoglobin, of the isolated alpha and beta chains, in the oxygenated forms, and of the oxymyoglobin and carp oxyhemoglobin have been measured at the wiggler beam line of the frascati synchrotron radiation facility. the bonding angle of oxygen molecule at the iron site in these hemoproteins in solution, has been measured using the multiple scattering theory for data analysis. | 1987 | 2443133 |
| sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies. | from 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached sepharose 4b. the antibodies bound to the agarose matrix were eluted with 0.5 m d-galactose containing buffers. using species-specific anti-immunoglobulin sera in immunoelectrophoresis, the immunoglobulin character of the protein fractions prepared was a ... | 1987 | 2444220 |
| contractile response to substance p in isolated smooth muscle strips from the intestinal bulb of the carp (cyprinus carpio). | 1. the effect of substance p on the mechanical activity of carp intestinal bulb smooth muscle was investigated in vitro. 2. bath-applied substance p (1 nm-1 microm) caused concentration-dependent contraction of the smooth muscle. the ec50 value was 20 +/- 3 nm (n = 13). 3. pretreatment with tetrodotoxin (780 nm) or atropine (500 nm) partially decreased the contractile response to substance p, while methysergide (3 microm) did not decrease the response. 4. the contractile response to substance p ... | 1988 | 2455618 |
| antigenic determinants on human choriogonadotropin alpha-subunit. ii. immunochemical mapping by a monoclonal antipeptide antibody. | in order to study antigenic site(s) present in the carboxyl-terminal part of the alpha-subunit of human choriogonadotropin (hcg-alpha), we attempted to produce site-specific antibodies directed against a 34-residue synthetic peptide analogous to region 59-92 of hcg-alpha. from a fusion experiment performed with a mouse injected with hcg-alpha-(59-92)-peptide conjugated to tetanus toxoid as immunogen, we selected a monoclonal antipeptide antibody (designated fa36) which has high binding activity ... | 1988 | 2455715 |
| effect of pesticides (atrazine and lindane) on the replication of spring viremia of carp virus in vitro. | atrazine and lindane are widely employed as pesticides in agriculture, which by wash off, could contaminate the aquatic environment. in this study, they were tested in vitro on the replication of the virus of spring viremia of carp (svc) in epithelioma papulosum cyprini cell line. they were dissolved first in dmso and then in medium at their solubility limit in water (28 and 10 mg/l). the results of the kinetic titers after 72 h showed no statistically significant difference between control and ... | 1988 | 2461139 |
| the immune response of carp against dextran. an analysis of the idiotype heterogeneity. | the expression of a "species-specific" idiotype with specificity for alpha(1-6) dextran in carp is described. all tested carp anti-dextran antisera react with a rabbit anti-id (carp anti-dex) serum. the rabbit anti-id serum can be partially inhibited by the specific ligand, dextran t70 or isomaltodecaose but not or only weak with other, unrelated carbohydrates. these data confirm our statement, that a limited germline v-gene repertoire may code for antibodies in fish. | 1988 | 2463185 |
| presence of a substance p-like peptide in an acid extract of the intestinal bulb of the carp (cyprinus carpio). | 1. the effect of an acid extract of the carp intestinal bulb (eci) on guinea-pig ileum longitudinal smooth muscle (gplm) and carp intestinal bulb longitudinal smooth muscle (ciblm) was examined. 2. eci caused a concentration-dependent contraction of gplm and ciblm. this eci-induced response was reduced by atropine to 30-40% of the control, indicating that part of the contracting activity of eci is attributable to acetylcholine. the atropine-resistant contracting activity of eci was not mediated ... | 1988 | 2464388 |
| anti-dextran antibodies of carp detected by the enzyme-linked immunosorbent assay (elisa). | carp produce anti-alpha (1-6) dextran antibodies after immunization with a vaccine of leuconostoc mesenteroides b512. these antibodies can be determined by passive haemagglutination, quantitative precipitation or by elisa. in our hands the elisa has proved to be 100 times more sensitive than the passive haemagglutination test. we could not found any correlation between the anti-dextran activity estimated with the passive haemagglutination on the one and the elisa on the other hand. | 1988 | 2465680 |
| optic nerve regeneration in adult fish and apolipoprotein a-i. | fish optic nerves, unlike mammalian optic nerves, are endowed with a high capacity to regenerate. injury to fish optic nerves causes pronounced changes in the composition of pulse-labeled substances derived from the surrounding non-neuronal cells. the most prominent of these injury-induced changes is in a 28-kilodalton (kda) polypeptide whose level increases after injury, as revealed by one-dimensional gel electrophoresis and autoradiography. the present study identified as apolipoprotein a-i (a ... | 1989 | 2494299 |
| structural differences between the ca2+-atpase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles. | 1. structural features were compared in sarcoplasmic reticulum ca2+-atpase enzymes from carp (cyprinus carpio l.) and rabbit muscles. 2. both membrane preparations contained the 105,000 mol. wt ca2+ pump protein in high local density. 3. the tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. addition of vanadate, ca2+ or lanthanides did not cause two-dimensional ca2+-atpase crystal formation, in contrast to the rabbit enzyme, which for ... | 1989 | 2522383 |
| the adductor mandibulae muscle in teleost fish with protrusible or non protrusible jaws: a histochemical and immunohistochemical study. | a study was made of the morphology and fibre type composition of the adductor mandibulae (am) muscle in teleosts with very protrusible (carp), moderately protrusible (cod) and non-protrusible (trout and cat-fish) jaws. in contrast to the trout and cat-fish, in which the am is formed by only 2 components (mandibular and mental), in the carp and cod there is a third portion (maxillary) which is more or less developed in relation to the extent of jaw protrusion. fibre types were identified by means ... | 1989 | 2527478 |
| inward rectifier produced by xenopus oocytes injected with mrna extracted from carp olfactory epithelium. | ionic channels encoded by mrna extracted from carp olfactory epithelium were investigated by injection into xenopus laevis oocytes. the oocytes expressed an inward rectifier k+ -channel, as detected under two-electrode voltage clamp conditions. the results were as follows. an inactivating inward current appeared on hyperpolarization and increased with increasing extracellular k+ concentrations. the 0 current potentials plotted as a function of log [k+]0 in the range between 2 to 20 mmk+ fell on ... | 1989 | 2541514 |
| spin equilibria in human methemoglobin: effects of bezafibrate and inositol hexaphosphate as measured by susceptometry and visible spectroscopy. | the effects of inositol hexaphosphate (ihp) and a second allosteric effector, bezafibrate, on the spin-state equilibria of the mixed-spin derivatives of ferric human hemoglobin a are examined. changes in spin-state equilibrium are monitored by measuring absorption spectra in the visible region (460-700 nm) as well as by direct measurements of magnetic susceptibility by means of a superconducting fluxmeter. the addition of ihp at ph 6.5 results in a measurable shift in the spin equilibria of thes ... | 1989 | 2548609 |
| spin equilibrium in human methemoglobin: effects of inositol hexaphosphate and bezafibrate as measured by resonance raman spectroscopy. | the spin equilibria of several derivatives of human methemoglobin were probed by resonance raman scattering. the intensity of lines in the raman spectrum gives a measure of the high-spin (s = 5/2) to low-spin (s = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility measurements. the addition of bezafibrate (bzf) to methemoglobin in the absence of organic phosphate, ihp, has very little effect on the spin equilibrium, whereas in the presence of ihp ... | 1989 | 2548610 |
| the sympathetic neuro-melanophore transmission in a fresh-water indian major carp, labeo rohita (ham.). | adrenaline was effective in aggregating the melanosomes both in innervated as well as denervated melanophores. isotonic kcl could induce pigment aggregation only in innervated melanophores. adrenaline- and k+-induced pigment aggregation response in these melanophores was blocked by phentolamine: propranolol failed to do so. it is suggested that the chromatic nerves in the fish, labeo rohita are adrenergic and via post-synaptic alpha-adrenoceptors, control the melanosome aggregation. | 1989 | 2550366 |
| distribution of angiotensin i converting enzyme in male reproductive systems of various vertebrates and properties of the genital enzymes. | the distribution of the angiotensin i converting enzyme (ec 3.4.15.1, ace) in male reproductive systems of various vertebrates including non-mammalian species and properties of the genital aces were studied. in such mammals as rat, dog and pig, it has been found that ace activity is only distributed in testis and epididymis (especially in the epididymal semen), but not in accessory sex glands such as prostate, seminal vesicle and coagulating gland. in the rat and dog, most of or all epididymal a ... | 1989 | 2558511 |
| production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain s-100 protein. | balb/c mice were immunized with s-100 protein, which was isolated from bovine brain. the first fusion resulted in the cloning of three stable hybridoma lines (s1-61-64, s1-61-65, s1-87-4) that produced monoclonal antibodies against s-100 protein. the hybridoma lines obtained from a second fusion (s2-20, s2-95) were not stable and antibody production ceased early during cloning. immunoblotting results showed that all antibodies reacted with antigenic determinants shared by both the alpha- and bet ... | 1985 | 2578587 |
| phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. | of eight monoclonal antibodies raised against human transferrin, one (h.tf-14) cross reacted with pig and rabbit transferrins and one (h.tf-1) showed cross-reactivity with horse and dog transferrins. while rabbit and pig transferrins exhibited the same patterns of binding to molt-3 cell receptors as human and horse transferrins, binding of mouse and dog transferrins was weaker and bovine and carp transferrins gave entirely negative results. the results of these competitive binding experiments we ... | 1985 | 2578807 |
| adenohypophysial cell types in an indian freshwater major carp, catla catla (hamilton). | the different adenocytes in an indian freshwater major carp, catla catla (hamilton) have been studied by employing various modern staining techniques. altogether, 8 different cell types have been reported in the adenohypophysis of the aforesaid fish. variations in the staining intensities of the cell types with the different techniques adopted have been detailed. | 1985 | 2581848 |
| comparison of xanthine: nad+ oxidoreductase from liver of toad bufo viridis and other vertebrates. | 1. xanthine oxidoreductase was isolated from toad bufo viridis (a mainly ureotelic amphibian species) and partially purified. the enzyme occurred as a stable xanthine: nad+ oxidoreductase (ec 1.1.1.204), unconvertible to the oxidase form. 2. some properties of the enzyme resembled those of xanthine oxidoreductase from an ammonotelic fish, cyprinus carpio, and the ureotelic rat, but in other aspects it was similar to this enzyme from an uricotelic snake, natrix natrix. 3. inhibition of the toad e ... | 1989 | 2591196 |
| aldehyde dehydrogenase activity in blood from various vertebrates. | 1. aldehyde dehydrogenase activity was determined in whole blood samples from 17 selected vertebrates of 5 classes, using 3,4-dihydroxyphenylacetaldehyde (the aldehyde derived from dopamine) as substrate. 2. aldehyde dehydrogenase activity in blood was widely but unevenly distributed among the species studied. 3. mean aldehyde dehydrogenase activities in the range of 40-140 nmol/min.ml blood (measured at 37 degrees c, ph 8.8) were found in blood from man, monkey, rabbit, guinea pig and mouse (c5 ... | 1989 | 2620490 |
| in vitro effect of mammalian pituitary hormones on germinal vesicle breakdown in oocytes of major carps, labeo rohita, cirrhinus mrigala, catla catla and cyprinus carpio. | among all the mammalian pituitary hormones, luteinizing hormone (lh) was the most potent in vitro inducer of oocyte maturation in l. rohita, c. mrigala, c. catla and c. carpio. it induced significant germinal vesicle breakdown (gvbd) at concentrations of 10, 1, 0.1 and 0.01 micrograms/ml. at the highest concentration used, lh induced 77.9 +/- 5.9, 73.8 +/- 4.6, 50.3 +/- 2.8 and 40.8 +/- 1.4% gvbd in oocytes of l. rohita, c. mrigala, c. catla and c. carpio, respectively. among other hormones, fol ... | 1989 | 2632393 |
| bacterial action of fertilization envelope extract from eggs of the fish cyprinus carpio and plecoglossus altivelis. | the vitelline envelope (ve) and fertilization envelope (fe) in eggs of the fish cyprinus carpio and plecoglossus altivelis were purified by homogenization of eggs or embryos in 5 mm tris-hcl buffer, ph 7.0, containing 2 mm ethylenediamine tetraacetic acid disodium salt (edta), except for processing of ves in plecoglossus eggs, and by repeated washing wih the same buffer. to extract the outermost layer material, the purified ves and fes were processed overnight at 4 degrees c in 5 mm tris-hcl buf ... | 1989 | 2661718 |
| new trends in parasitic infections of cultured freshwater fish. | the 1980s are characterized by the appearance of new fish parasitoses, the clarification of the aetiology of diseases and new insights into the process of infection and the development of important parasites, especially myxosporidians. two phaerosporoses have entered into the foreground: swimbladder inflammation (sbi) caused by sphaerospora renicola in common carp and proliferative kidney disease (pkd) in salmonids. both diseases must be reported in the german democratic republic (gdr). the conn ... | 1989 | 2669320 |
| immunohistochemical characterization of lymphocyte populations in carp (cyprinus carpio l.). | surface immunoglobulin-bearing cells from peripheral blood, thymus, head kidney, and spleen in carp (cyprinus carpio l.) were studied by immunoperoxidase labelling using antisera against fragments of carp igm. different lymphoid cell populations could be determined. thus, about 35% of the lymphocytes of the peripheral blood, head kidney, and spleen were determined, as "b" lymphocytes, using rabbit anti-carp fab antiserum with l chain specificity. nearly all lymphocytes of the peripheral blood, h ... | 1989 | 2673224 |
| [new data on some parasitic invasions in fish]. | the author gives a review of new literature data from the 80-ties. the following problems are considered: etiology of swim bladder inflammation in carp (sbi), development of parasites of the class myxosporea, overgrowth of parasitic kidney disease (pkd) in salmonid fishes, details of development of myxobolus cerebralis and ichthyophtirius multifiliis, and the new systematics of the diplozoidae. | 1989 | 2699131 |
| amino acid sequence of alpha-chain of hemoglobin iv from trout (salmo irideus). | the amino acid sequence of the alpha-chain of trout hemoglobin (hb) iv is given, thus completing the primary structure of the hemoglobin component of trout's blood characterized by the root effect. the trout hb iv alpha-chain consists of 142 amino acid residues; comparison with the corresponding sequences from human and carp hemoglobins shows differences of 50.0 and 35.9%, respectively. a difference of 39.6% is found with the alpha-chain of trout hb i, the other major hemoglobin component of tro ... | 1989 | 2706274 |
| [seasonal dynamics of the population count of bothriocephalus opsariichthydis (cestoda, pseudophyllidea) and the differential infestation of carp yearlings of various genotypes]. | | 1989 | 2717201 |
| hydrogen ion equilibria in fish haemoglobins. | h+ titration studies on oxygenated and deoxygenated haemoglobins from carp, rainbow trout, spiny dogfish and pig are reported, and compared with hb-h+ equilibria in other species and structural information deduced from amino acid sequences. the buffer values of oxygenated and deoxygenated teleost haemoglobins are low in comparison with elasmobranch and mammalian haemoglobins. this correlates with a much lower content of histidine residues and alpha-amino groups in teleost haemoglobins, than that ... | 1989 | 2732661 |
| lateral feedback from monophasic horizontal cells to cones in carp retina. i. experiments. | the spatial and color coding of the monophasic horizontal cells were studied in light- and dark-adapted retinae. slit displacement experiments revealed differences in integration area for the different cone inputs of the monophasic horizontal cells. the integration area measured with a 670-nm stimulus was larger than that measured with a 570-nm stimulus. experiments in which the diameter of the test spot was varied, however, revealed at high stimulus intensities a larger summation area for 520-n ... | 1989 | 2732679 |
| natural environment surpasses polluted environment in inducing dna damage in fish. | measurement of specific dna adduct concentrations in target tissues of organisms may provide a key biologic end-point of exposure to environmental carcinogens. using a general and highly sensitive assay with 32-p-postlabeling, we found that natural populations of freshwater fish species chub, barbel, bream and carp, as well as a marine fish mugil, revealed the presence of four to nine qualitatively similar adducts irrespective of whether they were caught from unpolluted or polluted waters. no st ... | 1989 | 2736723 |
| characterization of gamma-crystallins from eye lenses of shark: closer structural similarity to mammalian than other piscine gamma-crystallins? | lens crystallins were isolated and characterized from sharks of the cartilaginous fishes. four crystallin fractions corresponding to alpha-, beta h-, beta l- and gamma-crystallins, similar to those of mammalian crystallins, were obtained. the native molecular masses and subunit structures of these purified fractions were analyzed by gel permeation chromatography, sds gel electrophoresis and isoelectric focusing, revealing the typical subunit compositions with various extents of heterogeneity in ... | 1989 | 2737298 |
| [opisthorchiasis at left dnieper watersheds]. | present state of opisthorchosis infestation in the dnieper watershed (rivers vorskla, psyol, khorol, sula, seym, snov, desna water basins), situated in the sumy, chernigov, and poltava regions of the ukraine, is assessed. 7909 humans from 37 villages, 94 cats, 1134 carp fishes, 20013 mollusca and 30 soil samples were screened for the presence of opisthorchis eggs. opisthorchosis foci with an over 36% infection rate of population remain in the watersheds of the dnieper left confluents (rivers des ... | 1989 | 2755409 |
| development of trypanoplasma borreli (mastigophora: kinetoplastida) in the leech vector piscicola geometra and its infectivity for the common carp, cyprinus carpio. | the development of trypanoplasma borreli in the crop of the leech vector piscicola geometra was characterized by significant changes in morphology. immediately after ingestion by the leech, stumpy-shaped t. borreli predominated and numerous dividing specimens were found. this led to long and slender trypanoplasms near the end of the infection. the infection was terminated with complete digestion of the blood stored in the crop of the leech. the longest period of infection observed was 11 days. t ... | 1989 | 2760765 |
| an homologous radioimmunoassay for coho salmon (oncorhynchus kisutch) vitellogenin, with general applicability to other pacific salmonids. | this paper describes an homologous radioimmunoassay for coho salmon vitellogenin that demonstrates parallel cross-reactivity for plasma vitellogenin of all pacific salmonids tested (chinook, chum, coho, pink, and sockeye salmon, and cutthroat and rainbow trout), but not for atlantic salmon or two nonsalmonids: common carp and sablefish. plasma vitellogenin levels were high in ovulatory female pacific salmonids (micrograms/ml to mg/ml range), but were mostly nondetectable in spermiating males of ... | 1989 | 2767411 |
| an effective method for plasma lipoprotein separation: studies of various animal species. | 1. plasma lipoprotein separation by density gradient ultracentrifugation largely depends on visual examination based on the natural yellow pigments of lipoproteins. 2. in non-human species and in humans with dyslipoproteinemia, some lipoproteins are not well visualized due to the lack of pigments. 3. using a fluorescent probe in minute quantity (1,6 diphenyl-1,3,5-hexatriene) we were able to demonstrate an effective plasma lipoprotein separation using a discontinuous density gradient ultracentri ... | 1989 | 2776426 |
| [the infection process with sphaerospora renicola (myxosporidia)]. | new data are presented on the development of infection by s. renicola in common carp within the first 15 weeks of their life. the infection of parasite-free common carp succeeded by myxospores as well as by feeding carp kidneys with and without spores in laboratory experiments. in field experiments one year-old parasite-free common carp were successfully infected. | 1989 | 2782666 |
| [the dynamics of the immunologic indices of carp exposed to methylene blue]. | several immunological and haematological indices as well as infection of carp with ectoparasites of the dactylogyrus genus have been studied in fish that have been subjected to methylene blue treatment, to infection with rhabdovirus carpio and aeromonas hydrophila in different combinations. a dosage of 0.05 mg/l (exposure lasting for 3 days) over 20 days increases most of the immunological indices. a treatment of the carp with drug (dosage of 100 mg/l) for 4 hours has a stimulating effect on the ... | 1989 | 2792442 |
| the parasitemia of cloned trypanoplasma borreli laveran and mesnil, 1901, in laboratory-infected common carp (cyprinus carpio l.). | the course of parasitemia of cloned trypanoplasma borreli in laboratory-infected common carp was investigated. in 25-42-g carp kept at 20 c, the prepatent period was 8 days; after a phase of exponential growth, the parasitemia peaked at day 39 postinjection (pi) at a level of about 10(3) t. borreli/microliters blood. this maximum was followed by a chronic phase of about 6 wk with large numbers of t. borreli. at 20 wk pi, t. borreli was absent in infected carp. in 2.2-g carp kept at 20 c, the pre ... | 1989 | 2795371 |
| the 18p- syndrome. report of five cases. | five children, three males and two females were found to have a short arm deletion of chromosome 18. four of them display some of the typical features of this syndrome: microcephaly, round face, hypertelorism, broad-based nose, "carp-mouth", microrethrognathia, pterygium colli, dysplastic and low set ears, clinodactily, failure to grow, muscular hypotony and mental retardation. different hypotheses are discussed in order to explain the variable phenotypical expression of the 18 p-syndrome. | 1989 | 2817777 |
| carp (cyprinus carpio) heart has a high sensitivity to the positive inotropic effect of strophanthidin despite negative force-frequency relationships. | 1. the relationship between response of the heart to increased stimulation frequency and digitalis sensitivity was examined comparing the positive inotropic effect of strophanthidin and [3h]ouabain binding to sarcolemmal na+, k+-activated adenosine triphosphatase (na+, k+-atpase) in carp heart, which showed a negative force-frequency relationship, and in guinea-pig heart, which has a positive relationship. 2. in ventricular muscle preparations isolated from carp heart, strophanthidin increased d ... | 1987 | 2822523 |
| [characterization of opioid receptor from bullfrog brain]. | among the tested five species--carp, bullfrog chicken, rat, and mouse--the solubilized fraction derived from the bullfrog brain gave the highest binding activity similar to that of membranes. the difference in their binding activities was demonstrated to depend on the bmax value, but not on kd. high-affinity binding of [3h]naloxone and [3h]ethylketocyclazocine (ekc) was proved in supernatant of detergent-treated membranes. the displacement curves of various [3h]ligands binding to the membrane an ... | 1988 | 2851236 |
| amino acid sequence and three-dimensional structure of cytosolic adenylate kinase from carp muscle. | the amino acid sequence of cytosolic adenylate kinase from carp muscle has been determined. for the analysis of the blocked amino terminus an n-acylaminoacyl peptidase has been isolated and applied successfully. the resulting sequence is homologous to those of other adenylate kinases. it has 76% and 74% identical amino acids when compared with the enzymes from chicken and pig, respectively. the carp enzyme forms x-ray-grade crystals, the structure of which has been solved at 0.58-nm resolution. ... | 1988 | 2851785 |
| so-called interplexiform cells immunoreactive to tyrosine hydroxylase or somatostatin in rat retina. | the morphology of so-called interplexiform (ip) cells immunoreactive to tyrosine hydroxylase (th) or somatostatin (som) in the rat retina was described in comparison with those in the carp retina. in frozen cross-sections of the rat retina, many processes of th-like immunoreactive cells were found to extend toward the outer plexiform layer (opl), forming a thin layer of network fibers. a few of them further extended into the photoreceptor cell layer; such fibers were never found in the carp reti ... | 1985 | 2864980 |
| effects of acidic amino acid antagonists upon the spectral properties of carp horizontal cells: circuitry of the outer retina. | the acidic amino acid receptor antagonists, alpha-methylglutamate and alpha-aminoadipate, were applied to the carp retina to study their effects upon the spectral properties of horizontal cells and to elucidate the synaptic connections between horizontal cells and cones. application of these antagonists strongly hyperpolarized the l-type cone horizontal cells and reduced the responses of these horizontal cells to red light more than to blue light. application of co2+ ions to the retina, a proced ... | 1985 | 2865339 |
| histaminergic mechanisms in the vertebrate visual system. | this paper presents and summarizes our recent results on histamine (hi) in vertebrate visual system. the data include: 1. hi distribution, localization, synthesis, uptake and metabolism in retina, optic nerve, choroid and brain of carp, hen, rabbit, cow, 2. distribution of hi in human eyes, 3. 3h-mepyramine binding in the bovine retina, and hi dependent camp generating system in the rabbit eye, 4. effect of light stimulation on hi content in the rabbit retina, optic nerve and brain, 5. diurnal v ... | 1985 | 2870485 |
| beta-2 adrenergic receptors are not only for circulating catecholamines in ventricular muscles of carp heart (cyprinus carpio). | whether beta-2 adrenergic receptors are only for the circulating catecholamines in isolated ventricular muscles of carp heart was studied. isoproterenol, epinephrine and norepinephrine had concentration dependent positive inotropic effects. the ed50 values for isoproterenol, epinephrine and norepinephrine were 6.23 +/- 1.9, 87.3 +/- 27.3 and 4500 +/- 580 nm, respectively. phentolamine did not alter the inotropic effects. tyramine increases the force of contraction and this action was completely ... | 1986 | 2871987 |
| hormones with adrenocorticotropic and opiate-like activities from the carp (cyprinus carpio) pituitary. | carp (cyprinus carpio) pituitary acetone powder was extracted with a mixture of water, hydrochloric acid and acetone. an acid acetone powder was formed by adding the pituitary extract into a large volume of chilled acetone and subsequently recovering the precipitate. the powder was subjected to ion exchange chromatography on cm cellulose. fractions adsorbed on the ion exchanger exhibited acth-like activity as evidenced in the ability to stimulate lipolysis in isolated hamster adipocytes and cort ... | 1986 | 2879705 |
| carp maturational-ovulatory gonadotropin but not carp vitellogenic gonadotropin or salmon maturational-ovulatory gonadotropin stimulates testosterone production by rat leydig cells in vitro. | carp (cyprinus carpio) maturational-ovulatory gonadotropin, prepared from the fraction of pituitary extract adsorbed on con a-sepharose (con a ii) and subsequently adsorbed on cm-cellulose (whatman cm-52), stimulated testosterone production by isolated rat leydig cells. the fraction of carp pituitary extract unadsorbed on the immobilized lectin (con a i) with a mol. wt of 30,000, which had previously been shown to contain vitellogenic gonadotropin, was devoid of steroidogenic activity. salmon (o ... | 1987 | 2881654 |
| the interplexiform-horizontal cell system of the fish retina: effects of dopamine, light stimulation and time in the dark. | interplexiform cells contact cone horizontal cells in the fish retina and probably release dopamine at synaptic sites. the effects of dopamine, certain related compounds, and light and dark régimes were tested on the intracellularly recorded activity of horizontal cells in the superfused carp retina to elucidate the functional role of the interplexiform cell. dopamine application onto retinae kept in the dark for 30-40 min increased the size of the responses of cone horizontal cells to small-spo ... | 1987 | 2888119 |
| metabolic activation of 2-aminofluorene, 2-acetylaminofluorene and n-hydroxy-acetylaminofluorene to bacterial mutagens with mussel (mytilus galloprovincialis) and carp (cyprinus carpio) subcellular preparations. | 1. the mode of activation of 2-aminofluorene (af), 2-acetylaminofluorene (aaf) and n-hydroxy-acetylaminofluorene (oh-aaf) to salmonella typhimurium ta 98 mutagens was investigated in subcellular fractions from the digestive gland of the mussel mytilus galloprovincialis and from the liver of carp cyprinus carpio. 2. in carp liver microsomes the activation of oh-aaf was due to very active deacetylase, in contrast to undetectable deacetylase-dependent activation in mussel microsomes. 3. af and aaf ... | 1987 | 2890481 |
| a d-mannose-specific lectin from gerardia savaglia that inhibits nucleocytoplasmic transport of mrna. | a new lectin has been isolated from the coral gerardia savaglia by affinity chromatography, using locust gum as an absorbent, and d-mannose as eluant. final purification was achieved by bio-gel p300 gel filtration. the agglutinin is a protein composed of two polypeptide chains with a mr of 14800; the two subunits are not linked by disulfide bond(s). the isoelectric point is 4.8, the amino acid composition is rich in the acidic amino acids aspartic acid and glutamic acid. the absorption maximum f ... | 1987 | 2890521 |
| scrapie-associated fibrils, prp protein and the sinc gene. | scrapie-associated fibrils (saf) are disease-specific structures found in extracts of the brains of animals affected with scrapie. these structures are pathological aggregates of a normal host protein called prp. in collaboration with konrad beyreuther (heidelberg), we have characterized the multiple forms of prp found in saf fractions from mouse brain affected by the me7 strain of scrapie. there is no in vivo n-terminal cleavage of the most abundant forms of prp. however, n-terminal cleavage of ... | 1988 | 2900718 |
| purification of carp growth hormone and cloning of the complementary dna. | the growth hormone (gh) was isolated and purified from common carp (cyprinus carpio) pituitary glands by salt precipitation and hplc on reverse-phase c18 columns. the carp gh cdna was synthesized and cloned in escherichia coli using ecori linkers and pbr322 as vector. the positive clones were selected and sequenced. the full-length carp gh cdna contains 1187 nucleotide basepairs with an open reading frame coding for the precursor form carp gh of 210 amino-acid residues. the partial amino-acid se ... | 1989 | 2920175 |
| age-dependent changes in volume and haemoglobin content of erythrocytes in the carp (cyprinus carpio l.). | carp erythrocytes were fractionated by angle-head centrifugation which yielded fractions with a linear increase in density. haematological examinations revealed that the heavier red blood cells of carp had greater volumes (mcv), more haemoglobin (mch) and higher haemoglobin concentrations (mchc) than light ones. the same experiments with human red cell fractions yielded a decrease in mcv, constant mch and an increase in mchc. haemoglobin content in individual erythrocytes was also determined by ... | 1989 | 2926316 |