| fungicidal activity of beta-thujaplicin analogues. | the fungicidal activity of analogues of beta-thujaplicin, a natural product responsible for the durability of heartwood of several cupressaceous trees, was investigated in vitro on the growth of different white and brown rot fungi involved in wood biodegradation, coriolus versicolor, phanerochaete chrysosporium, poria placenta and gloephyllum trabeum. the study shows that 2-hydroxycyclohepta-2,4,6-trienone (tropolone), easily prepared according to a literature procedure, possesses interesting fu ... | 2001 | 11561410 |
| entrapment of white-rot fungus trametes versicolor in ca-alginate beads: preparation and biosorption kinetic analysis for cadmium removal from an aqueous solution. | the biosorption of cadmium ions onto entrapped trametes versicolor mycelia has been studied in a batch system. the maximum experimental biosorption capacities for entrapped live and dead fungal mycelia of t. versicolor were found as 102.3 +/- 3.2 mg cd(ii) g(-1) and 120.6 +/- 3.8 mg cd(ii) g(-1), respectively. biosorption equilibrium was established in about 1 h and biosorption was well described by the langmuir and freundlich biosorption isotherms. the change in the biosorption capacity with ti ... | 2001 | 11563702 |
| evaluation of polysaccharopeptide effects against c6 glioma in combination with radiation. | long-term control of high-grade brain tumors is rarely achieved with current therapeutic regimens. the major goal of this study was to determine whether polysaccharopeptide (psp), a crude polysaccharide peptide extract derived from coriolus versicolor, a fungus, could enhance the effects of radiation against glioma cells in culture and in xenografted tumors in vivo. psp significantly augmented radiation-induced damage to c6 rat glioma cells in vitro. nude mice injected subcutaneously with the c6 ... | 2001 | 11574781 |
| examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases. | a variety of lectins were tested in vitro for inhibitory action against the activities of human immunodeficiency virus type 1 (hiv-1) reverse transcriptase and the n-glycohydrolases (alpha-glucosidase, beta-glucosidase and beta-glucuronidase). lectins from phaseolus vulgaris, momordica charantia, ricinus communis and its constituent chains, and agaricus bisporus were able to inhibit hiv-1 reverse transcriptase. p. vulgaris lectin and a. bisporus lectin were the most potent. the aforementioned le ... | 2001 | 11582548 |
| decolorization of reactive dyes by the white rot fungus trametes versicolor in sequencing batch reactors. | the white rot fungus trametes versicolor was shown to be capable of decolorizing three reactive dyes in a sequencing batch process, using glucose as the carbon and energy source over an extended period without supplementation of new mycelium. decolorization activity was related to the expression of extracellular peroxidases and could be continuously reactivated by sheering the suspended pellets. pure culture experiments were carried out simultaneously in agitated erlenmeyer flasks and in complet ... | 2001 | 11590604 |
| transformation of 2,4,6-trichlorophenol by free and immobilized fungal laccase. | laccase from the white rot fungus coriolus versicolor was immobilized on celite r-637 by covalent binding with glutaraldehyde. after a sharp primary decline in activity (up to 50%), the retained enzyme activity was stable over a storage period of 33 days at 4 degrees c. a comparative study of soluble and immobilized laccases revealed the increased resistance of immobilized enzyme to the unfavourable effects of alkaline ph, high temperature and the action of inhibitors. a combination of these pro ... | 2001 | 11693939 |
| degradation of diphenyl ether herbicides by the lignin-degrading basidiomycete coriolus versicolor. | under ligninolytic conditions, the white-rot basidiomycete coriolus versicolor metabolized chloronitrofen (2, 4, 6-trichloro-4'-nitrodiphenyl ether; cnp) and nitrofen (2, 4-dichloro-4'-nitrodiphenyl ether, nip), which constitute the largest class of commercially produced diphenyl ether herbicides. the pathway of cnp degradation was elucidated by the identification of fungal metabolites upon addition of cnp and its metabolic intermediates. the metabolic pathway was initially branched to form four ... | 2001 | 11762605 |
| the effect of aeration on the biotransformation of lignocellulosic wastes by white-rot fungi. | the mineralisation and the humification of organic matter (om) in sterile horticultural plant wastes inoculated with coriolus versicolor or phanerochaete flavido-alba was investigated under different aeration rates in order to determine their efficacy as potential inoculants for composting. the change in elemental composition, lignin content and om fractions was analysed during a 90-day incubation. both fungi degraded 30% of lignin at low aeration rates. different aeration rates led to significa ... | 2002 | 11762904 |
| purification and preliminary crystallographic study of trametes versicolor laccase in its native form. | laccases are multi-copper oxidases that catalyse the oxidation of a wide range of phenols and their use in industrial oxidative processes is increasing. a laccase has been purified from the fungus trametes versicolor and crystallized using the hanging-drop method. crystals belong to the monoclinic space group p2(1), with unit-cell parameters a = 87.7, b = 110.5, c = 123.2 a, beta = 103.4 degrees. a complete data set was collected to 2.4 a resolution on a cu k(alpha) rotating-anode x-ray source. ... | 2002 | 11807263 |
| purification, crystallisation and x-ray diffraction study of fully functional laccases from two ligninolytic fungi. | laccase isozymes from the white-rot basidiomycete fungi trametes versicolor and pycnoporus cinnabarinus were purified to apparent iso-electric homogeneity and crystallised. t. versicolor laccase crystallises in two crystal forms, both with the orthorhombic space group p2(1)2(1)2(1), which diffract to 1.9 and 2.95 a resolution, respectively. the crystals of p. cinnabarinus laccase belong to the monoclinic space group c2 and diffract to at least 2.2 a resolution. all the laccase crystals are suita ... | 2002 | 11825613 |
| identification and characterization of novel cytochrome p450 genes from the white-rot basidiomycete, coriolus versicolor. | using a reverse-transcription polymerase chain reaction (rt-pcr) technique, cytochrome p450 genes were cloned from the lignin-degrading basidiomycete, coriolus versicolor. one possible p450 gene was identified, which consisted of 1,672 nucleotides and a poly(a) tail and encoded a deduced protein containing 449 amino acids. the deduced amino acid sequence revealed the presence of the p450 heme-binding motif, strongly suggesting that this protein belongs to the p450 superfamily, then designated cy ... | 2002 | 11831480 |
| differential regulation of mnp2, a new manganese peroxidase-encoding gene from the ligninolytic fungus trametes versicolor prl 572. | a peroxidase-encoding gene, mnp2, and its corresponding cdna were characterized from the white-rot basidiomycete trametes versicolor prl 572. we used quantitative reverse transcriptase-mediated pcr to identify mnp2 transcripts in nutrient-limited stationary cultures. although mnp2 lacks upstream metal response elements (mres), addition of mnso(4) to cultures increased mnp2 transcript levels 250-fold. in contrast, transcript levels of an mre-containing gene of t. versicolor, mnp1, increased only ... | 2002 | 11916737 |
| metabolic response against sulfur-containing heterocyclic compounds by the lignin-degrading basidiomycete coriolus versicolor. | the fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete coriolus versicolor. the fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. under primary metabolic conditions, c. versicolor utilized thiophenes, such as 2-hydroxymethyl-, 2-formyl-, and 2-carboxyl-thiophenes, as a nutrient sulfur source for growth; thus, the fungus degraded these compounds more ... | 2002 | 11954800 |
| effect of synthetic and natural culture media on laccase production by white rot fungi. | laccase is among the major enzymes of white rot fungi involved in lignocellulose degradation. the present paper reports its production by two white rot fungi (coriolus versicolor, funalia trogii) under different nutritional conditions. various synthetic culture media and natural culture medium (molasses wastewater) were tested. enzyme production in various synthetic culture media, molasses wastewater (vinasse) culture medium and in the absence or presence of cotton stalk supplements showed that ... | 2002 | 11991068 |
| decolorization of textile dyes by laccases from a newly isolated strain of trametes modesta. | four ligninolytic fungi, trametes modesta, trametes hirsuta, trametes versicolor and sclerotium rolfsii, were compared for their ability to produce laccases. the fungal laccases were screened for their ability to decolorize eight synthetic dyes (anthraquinone, azo, indigo and triarylmethane). the decolorization rate depended both on the source of the enzyme preparation and on the structure of the dye. based on laccase production and dye decolorizing ability, t. modesta was selected for further s ... | 2002 | 11996335 |
| improving laccase production by employing different lignocellulosic wastes in submerged cultures of trametes versicolor. | laccase production by the white-rot fungus trametes versicolor (cbs100.29) grown in submerged cultures was studied. addition of different insoluble lignocellulosic materials into the culture medium in order to enhance laccase production was investigated. the lignocellulosic materials were grape seeds, grape stalks and barley bran, selected because of their availability and low cost, since they are agro-industrial wastes abundant in most countries. barley bran gave the highest activities, a maxim ... | 2002 | 12003310 |
| transformation of the medicinal basidiomycete trametes versicolor to hygromycin b resistance by restriction enzyme mediated integration. | trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. there have been many reports about the cloning of laccase and peroxidase genes of t. versicolor which are involved in lignin degradation. in order to analyze a gene function and introduce foreign genes into an organism, genetic transformation is required. here we have successfully transformed t. versicolor to hygromycin b resistance using pan 7-1 plasmid by restriction enzyme med ... | 2002 | 12007817 |
| influence of odor from wood-decaying fungi on host selection behavior of deathwatch beetle, xestobium rufovillosum. | adult females of xestobium rufovillosum de geer demonstrated anemotactic orientation when exposed to an odor plume containing volatiles generated by wood-decaying fungi (coriolus versicolor, donkioporia expansa) and decayed oak wood (quercus petraea, quercus robur). they did not orient towards undecayed oak wood, beech (fagus sylvatica), or pine wood (pinus sylvestris). although all insects tested showed anemotactic orientation, responses were nonlinear with respect to insect age. adult females ... | 2002 | 12035923 |
| crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics. | laccases are multicopper oxidases that catalyze the oxidation of a wide range of phenols or arylamines, and their use in industrial oxidative processes is increasing. we purified from the white rot fungus trametes versicolor a laccase that exists as five different isozymes, depending on glycosylation. the 2.4 a resolution structure of the most abundant isozyme of the glycosylated enzyme was solved. the four copper atoms are present, and it is the first crystal structure of a laccase in its activ ... | 2002 | 12044164 |
| effects of coriolus versicolor polysaccharide b on monocyte chemoattractant protein 1 gene expression in rat. | to investigate the effect of coriolus versicolor polysaccharide b (cvps-b), a new water-soluble component of polysaccharides from the fungus coriolus versicolor (fr) l on monocyte chemoattractant protein-1 (mcp-1) gene expression in rat splenocytes. | 2002 | 12060529 |
| degradation of contrasting pesticides by white rot fungi and its relationship with ligninolytic potential. | the capacity of nine species of white rot fungus from a variety of basidiomycete orders to degrade contrasting mono-aromatic pesticides was investigated. there was no relationship between degradation of the dye poly r-478, a presumptive test for ligninolytic potential, and degradation of the highly available pesticides diuron, metalaxyl, atrazine or terbuthylazine in liquid culture. however, there were significant positive correlations between the rates of degradation of the different pesticides ... | 2002 | 12076788 |
| expression of a gene for mn-peroxidase from coriolus versicolor in transgenic tobacco generates potential tools for phytoremediation. | in efforts aimed at the detoxification of contaminated areas, plants have many advantages over bacteria and fungi. we are attempting to enhance the environmental decontamination functions of plants by transferring relevant genes from microorganisms. when the gene for mn-peroxidase (mnp) from coriolus versicolor was expressed in transgenic tobacco plants, one line (designated fmnp21) expressed mnp activity at levels 54-fold higher than in control lines. when undamaged roots of transgenic plants w ... | 2002 | 12111153 |
| fermentation strategies for improved heterologous expression of laccase in pichia pastoris. | improved expression of recombinant laccase by pichia pastoris carrying the lcc1 cdna isolated from trametes versicolor was achieved by optimization of the cultivation conditions in a fermentor equipped with a methanol sensor system. the results indicated that the activity obtained in fermentor cultivations was at least 7 times higher than in shake-flask cultures. three different strategies for fermentor cultivations were compared: a (30 degrees c, 1.0% methanol), b (20 degrees c, 1.0% methanol), ... | 2002 | 12115407 |
| uptake of cadmium ions in white-rot fungus trametes versicolor: effect of cd (ii) ions on the activity of laccase. | the effects of cadmium cd (ii) ions on the physiology and biological activity of trametes versicolor, a strain belonging to white-rotting basidiomycetes, were examined. cd (ii) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. our experiments showed that t. versicolor is a good cadmium biosorbent from aqueous solution, this strain removing almost all the cd (ll) ions over the first 2h of incubation by what appears to be a rapid, energy-independent su ... | 2002 | 12127940 |
| optimisation of the expression of a trametes versicolor laccase gene in pichia pastoris. | a cdna encoding a laccase enzyme was isolated from a trametes versicolor cdna library. the gene was subcloned into the pichia pastoris expression vector ppic3.5 and transformed into the p. pastoris strains km71 and gs115. laccase-secreting transformants were selected by their ability to oxidise the substrate abts. no difference in laccase activity was observed between culture supernatants from gs115 (proteolytic) and km71 (nonproteolytic) strains. the presence of at least 200 microm copper was n ... | 2002 | 12161771 |
| crystal structure of a laccase from the fungus trametes versicolor at 1.90-a resolution containing a full complement of coppers. | laccase is a polyphenol oxidase, which belongs to the family of blue multicopper oxidases. these enzymes catalyze the one-electron oxidation of four reducing-substrate molecules concomitant with the four-electron reduction of molecular oxygen to water. laccases oxidize a broad range of substrates, preferably phenolic compounds. in the presence of mediators, fungal laccases exhibit an enlarged substrate range and are then able to oxidize compounds with a redox potential exceeding their own. until ... | 2002 | 12163489 |
| anticancer effects and mechanisms of polysaccharide-k (psk): implications of cancer immunotherapy. | polysaccharide-k (polysaccharide-kureha; psk), also known as krestin, is a unique protein-bound polysaccharide, which has been used as a chemoimmunotherapy agent in the treatment of cancer in asia for over 30 years. psk and polysaccharopeptide (psp) are both protein-bound polysaccharides which are derived from the cm-101 and cov-1 strains of the fungus coriolus versicolor by japanese and chinese researchers, respectively. both polysaccharide preparations have documented anticancer activity in vi ... | 2002 | 12168863 |
| biological activity of 4-acetyltropolone, the minor component of thujopsis dolabrata sieb. et zucc. hondai mak. | 4-acetyltropolone, a minor component of thujopsis dolabrata sieb. et zucc. hondai makino, showed antimicrobial activity against various microorganisms including wood-rotting fungi, a phytogrowth-inhibitory effect with chlorophyll biosynthesis inhibition, cytotoxic effect and inhibitory activity on metalloproteases. this compound had strong antifungal activity on daedalea dickinsii ifo-4979 [minimum inhibitory concentration (mic): 0.2 microg/ml] and coriolus versicolor ifo-4940 (mic: 0.39 microg/ ... | 2002 | 12186430 |
| coriolus versicolor: a medicinal mushroom with promising immunotherapeutic values. | coriolus versicolor (cv) is a medicinal mushroom widely prescribed for the prophylaxis and treatment of cancer and infection in china. in recent years, it has been extensively demonstrated both preclinically and clinically that aqueous extracts obtained from cv display a wide array of biological activities, including stimulatory effects on different immune cells and inhibition of cancer growth. the growing popularity of aqueous cv extracts as an adjunct medical modality to conventional cancer th ... | 2002 | 12211223 |
| structure of genes for hsp30 from the white-rot fungus coriolus versicolor and the increase of their expression by heat shock and exposure to a hazardous chemical. | we isolated and analysed two genomic dnas that encode the heat-shock protein hsp30 from coriolus versicolor. the amino acid sequences substitute only three amino acid substitutions. the promoter regions contain the consensus heat-shock element, a xenobiotic-response element, a stress-response element, and a metal-response element. the levels of mrnas for hsp30 increased markedly after exposure of c. versicolor to pentachlorophenol and levels were higher than those after heat shock. | 2002 | 12224644 |
| identification and heterologous expression of the cytochrome p450 oxidoreductase from the white-rot basidiomycete coriolus versicolor. | a cdna encoding cytochrome p450 oxidoreductase (cpr) from the lignin-degrading basidiomycete coriolus versicolor was identified using rt-pcr. the full-length cdna consisted of 2,484 nucleotides with a poly(a) tail, and contained an open reading frame. the g+c content of the cdna isolated was 60%. a deduced protein contained 730 amino acid residues with a calculated molecular weight of 80.7 kda. the conserved amino acid residues involved in functional domains such as fad-, fmn-, and nadph-binding ... | 2002 | 12226721 |
| redox-mediated decolorization of synthetic dyes by fungal laccases. | laccases from the lignin-degrading basidiomycetes trametes versicolor, polyporus pinisitus and the ascomycete myceliophthora thermophila were found to decolorize synthetic dyes to different extents. differences were attributed to the specific catalytic properties of the individual enzymes and to the structure of the dyes. due to their higher oxidative capacities, the laccases from the two basidiomycetes decolorized dyes more efficiently than that of the ascomycete. the azo dye direct red 28, the ... | 2002 | 12226723 |
| antioxidant properties of several medicinal mushrooms. | three species of medicinal mushrooms are commercially available in taiwan, namely, ganoderma lucidum (ling-chih), ganoderma tsugae (sung-shan-ling-chih), and coriolus versicolor (yun-chih). methanolic extracts were prepared from these medicinal mushrooms and their antioxidant properties studied. at 0.6 mg/ml, g. lucidum, g. lucidum antler, and g. tsugae showed an excellent antioxidant activity (2.30-6.41% of lipid peroxidation), whereas c. versicolor showed only 58.56%. at 4 mg/ml, reducing powe ... | 2002 | 12358482 |
| laccase production by some phlebia species. | the present study was carried out to examine the ability of four species of the genus phlebia, viz. p. floridensis, p. brevispora, p. radiata and p. fascicularia, to produce the ligninolytic enzyme laccase in liquid culture. p. floridensis was the most active species that even surpassed laccase production by trametes versicolor, and was chosen for further study. among several carbon sources tested, malt extract turned out to be the best medium for subsequent laccase concentration by ammonium sul ... | 2002 | 12362400 |
| molecular analysis of arylalcohol dehydrogenase of coriolus versicolor expressed against exogenous addition of dibenzothiophene derivatives. | using the differential display reverse-transcriptional polymerase chain reaction (ddrt-pcr) technique, several cdna fragments were isolated as chemical stress responsive genes from the white-rot basidiomycete, coriolus versicolor, exposed to either 4-methyldibenzothiophene-5-oxide (4mdbto) or dibenzothiophene-5-oxide (dbto). a database search on deduced amino acid sequences of cdnas revealed that they showed a high similarity with various proteins from other organisms. these results strongly sug ... | 2002 | 12362404 |
| biodegradation of aromatic compounds by white rot and ectomycorrhizal fungal species and the accumulation of chlorinated benzoic acid in ectomycorrhizal pine seedlings. | the capability of different white rot (wr, heterobasidion annosum, phanerochaete chrysosporium, trametes versicolor) and ectomycorrhizal (ecm, paxillus involutus, suillus bovinus) fungal species to degrade different aromatic compounds and the absorption of 3-chlorobenzoic acid (3-cba) by ecm pine seedlings was examined. the effect of aromatic compounds on the fungal biomass development varied considerably and depended on (a) the compound, (b) the external concentration, and (c) the fungal specie ... | 2002 | 12363308 |
| evaluation of support materials for the surface immobilization and decoloration of amaranth by trametes versicolor. | the ability of trametes versicolor atcc 20869 to colonize several natural and synthetic materials (wheat straw, jute, hemp, maple woodchips, and nylon and polyethylene teraphthalate fibers) and to subsequently decolorize amaranth was evaluated. jute was found to be the best support material as t. versicolor grew well on it without color leaching from the support and without loss of the jute's integrity over a 4 week period. the fungus immobilized on jute, straw and hemp decolorized amaranth (50 ... | 2002 | 12382067 |
| transformation of textile dyes by white-rot fungus trametes versicolor. | we have investigated transformation of eight industrial dyes by a white-rot fungus, trametes versicolor. the fungus was found to decolorize reactive golden yellow r, procion red, reactive violet 5, reactive blue 28, and ponceau red 4r at an initial dye concentration of 80 ppm within 72 h of incubation, whereas it took 5 d to completely decolorize reactive black 5 (40 ppm). however, it did not significantly decolorize reactive red 152 and novatic blue bc s/d. during decolorization in liquid mediu ... | 2002 | 12396114 |
| purification and characterization of an extracellular laccase from the edible mushroom lentinula edodes, and decolorization of chemically different dyes. | a laccase (ec 1.10.3.2) was isolated from the culture filtrate of lentinula edodes. the enzyme was purified to a homogeneous preparation using hydrophobic, anion-exchange, and size-exclusion chromatographies. sds-page analysis showed the purified laccase, lcc 1, to be a monomeric protein of 72.2 kda. the enzyme had an isoelectric point of around ph 3.0. the optimum ph for enzyme activity was around 4.0, and it was most active at 40 degrees c and stable up to 35 degrees c. the enzyme contained 23 ... | 2002 | 12436315 |
| effects of extracts of coriolus versicolor (i'm-yunity) on cell-cycle progression and expression of interleukins-1 beta,-6, and -8 in promyelocytic hl-60 leukemic cells and mitogenically stimulated and nonstimulated human lymphocytes. | the goal of this in vitro study was to test the cytostatic and cytotoxic activities of extracts derived from the polysaccharopeptide (psp), i'm-yunity (integrated chinese medicine holdings ltd., kowloon, hong kong) prepared from strain cov-1 of the mushroom coriolus versicolor. | 2002 | 12470440 |
| identification and functional expression of taha, a filamentous fungal gene involved in copper trafficking to the secretory pathway in trametes versicolor. | in this study, cdna and genomic clones encoding a homologue of the yeast gene anti-oxidant 1 (atx1) from the white-rot fungus trametes versicolor, a basidiomycete known to produce several laccase isoenzymes involved in lignin degradation, were identified. this gene, named trametes atx homologue (taha), encodes a protein of 7.9 kda with 56% identity to the yeast atx1p sequence. two different alleles of taha were obtained that differed mainly in their intervening sequences and in a 425 nt insertio ... | 2002 | 12480908 |
| reaction conditions for laccase catalyzed polymerization of catechol. | poly(catechol) was synthesized in batch runs with laccase from trametes versicolor (atcc 200801). the polymerization reaction was conducted in a closed, temperature controlled system containing acetone and sodium acetate buffer for ph control. the effects of the solvent mixture, monomer (catechol), enzyme, medium ph and temperature on the polymerization rate were investigated with respect to initial reaction conditions and depletion rate of dissolved oxygen in the medium. maximum initial reactio ... | 2003 | 12507858 |
| effect of culture conditions on manganese peroxidase production and activity by some white rot fungi. | the ligninolytic system of white rot fungi is primarily composed of lignin peroxidase, manganese peroxidase (mnp) and laccase. the present work was carried out to determine the best culture conditions for production of mnp and its activity in the relatively little-explored cultures of dichomitus squalens, irpex flavus and polyporus sanguineus, as compared with conditions for phanerochaete chrysosporium and coriolus versicolor. studies on enzyme production under different nutritional conditions r ... | 2003 | 12545383 |
| [decolorization of dyestuff and dying waste water by laccase solution with self-flocculent mycelial pellets of coriolus versicolor]. | both laccase production by the white-rot fungus coriolus versicolor and decolorization of dyestuff and dying waste water with crude solution of laccase were studied in this work. laccase production meets the definition of secondary metabolism. for laccase production the optimum initial ph is 4.5. addition of veratryl alcohol or elevated trace metals could both enhance the laccase activity, while tween80 showed some inhibition. the immobilized mycelia of c. versicolor in polyurethane foam had les ... | 2002 | 12557381 |
| dibenzyl sulfide metabolism by white rot fungi. | microbial metabolism of organosulfur compounds is of interest in the petroleum industry for in-field viscosity reduction and desulfurization. here, dibenzyl sulfide (dbs) metabolism in white rot fungi was studied. trametes trogii uamh 8156, trametes hirsuta uamh 8165, phanerochaete chrysosporium atcc 24725, trametes versicolor ifo 30340 (formerly coriolus sp.), and tyromyces palustris ifo 30339 all oxidized dbs to dibenzyl sulfoxide prior to oxidation to dibenzyl sulfone. the cytochrome p-450 in ... | 2003 | 12571066 |
| [resources and utilization of anticarciogenic medical fungi]. | this paper summarizes the resources of anticarcinogenic medical fungi, and the information of distribution, eco-environment. it outlines the present statuts of the exploitation and utilization of some species of common anticarcinogenic medical fungi such as ganoderma lucidum, coriolus versicolor, polyporus frondosus etc. the anticarcinogenic mechanism of medical fungi is discussed. suggestions on how to development and utilize rationally these resources are offered as well. | 1999 | 12571901 |
| [studies on chemical structure of polysaccharide from fruit body of coriolus versicolor]. | to study the structure of polysaccharides from coriolus versicolor. | 2001 | 12580103 |
| production of laccase by coriolus versicolor and its application in decolorization of dyestuffs: (ii). decolorization of dyes by laccase containing fermentation broth with or without self-immobilized mycelia. | the capability of decolorization for commercial dyes by coriolus versicolor fermentation broth containing laccase with or without immobilized mycelium was evaluated. with cell-free fermentation broth containing laccase, high decolorization ratio was achieved foracid orange 7, but not for the other dyes concerned. the immobilized mycelium was proved to be more efficient than the cell-free system. all the four dyestuffs studied were found being decolourized with certain extent by immobilized mycel ... | 2003 | 12602596 |
| production of laccase by coriolus versicolor and its application in decolorization of dyestuffs: (i). production of laccase by batch and repeated-batch processes. | the production of laccase by coriolus versicolor was studied. the effect of cultivation conditions on laccase production by coriolus versicolor was examined to obtain optimal medium and cultivation conditions. both batch and repeated-batch processes were performed for laccase production. in repeated-batch fermentation with self-immobilized mycelia, total of 14 cycles were performed with laccase activity in the range between 3.4 and 14.8 u/ml. | 2003 | 12608358 |
| competition strategies for the decolorization of a textile-reactive dye with the white-rot fungi trametes versicolor under non-sterile conditions. | a variety of white-rot fungi can oxidize textile dyes under sterile conditions; however, an important consideration for their use in treating wastewater containing textile dyes is whether similar degrees of treatment can be achieved under non-sterile conditions. four strategies were investigated for their potential in optimizing the use of the fungus trametes versicolor in non-sterile culture for treating wastewater containing the diazo textile dye c.i. reactive black 5 (rb5). three strategies w ... | 2003 | 12673774 |
| comparative biosorption of mercuric ions from aquatic systems by immobilized live and heat-inactivated trametes versicolor and pleurotus sajur-caju. | trametes versicolor and pleurotus sajur-caju mycelia immobilized in ca-alginate beads were used for the removal of mercuric ions from aqueous solutions. the sorption of hg(ii) ions by alginate beads and both immobilized live and heat-killed fungal mycelia of t. versicolor and p. sajur-caju was studied in the concentration range of 0.150-3.00 mmol dm(-3). the biosorption of hg(ii) increased as the initial concentration of hg(ii) ions increased in the medium. maximum biosorption capacities for pla ... | 2003 | 12699933 |
| cleavage of beta,beta-carotene to flavor compounds by fungi. | more than 50 filamentous fungi and yeasts, known for de novo synthesis or biotransformation of mono-, sesqui-, tri-, or tetraterpenes, were screened for their ability to cleave beta,beta-carotene to flavor compounds. ten strains discolored a beta,beta-carotene-containing growth agar, indicating efficient degradation of beta,beta-carotene. dihydroactinidiolide was formed as the sole conversion product of beta,beta-carotene in submerged cultures of ganoderma applanatum, hypomyces odoratus, kuehner ... | 2003 | 12719936 |
| carbon repression of cellobiose dehydrogenase production in the white rot fungus trametes versicolor is mediated at the level of gene transcription. | cellobiose dehydrogenase (cdh) production in trametes versicolor is induced in the presence of cellulose, but decreases when additional carbon sources such as glucose and maltose are added to the fungal cultures. using t. versicolor-specific cdh primers in a reverse transcription-polymerase chain reaction-based approach, it appears that this repression in cdh production is being mediated at the level of gene transcription. when a 1.6-kb upstream region of the t. versicolor cdh gene was cloned an ... | 2003 | 12725922 |
| oxalate production by wood-rotting fungi growing in toxic metal-amended medium. | in this report, we have identified oxalic acid as an important metabolite elaborated in the response of wood-rotting fungi to toxic metal stress. the formation of oxalate crystals by white rot fungi (bjerkandera fumosa, phlebia radiata and trametes versicolor) and the brown rot fungus fomitopsis pinicola, grown on media containing high levels of toxic metal ions has been visualized using scanning electron microscopy (sem) with energy-dispersive x-ray micro-analysis (edxa) and hplc. there were no ... | 2003 | 12738291 |
| comparative studies of fungal degradation of single or mixed bioaccessible reactive azo dyes. | a screening using several fungi (phanerochaete chrysosporium, pleurotus ostreatus, trametes versicolor and aureobasidium pullulans) was performed on the degradation of syringol derivatives of azo dyes possessing either carboxylic or sulphonic groups, under optimized conditions previously established by us. t. versicolor showed the best biodegradation performance and its potential was confirmed by the degradation of differently substituted fungal bioaccessible dyes. enzymatic assays (lignin perox ... | 2003 | 12781230 |
| coriolus versicolor polysaccharide peptide slows progression of advanced non-small cell lung cancer. | non-small cell lung cancer (nsclc) is a leading cause of cancer deaths, and over 60% of patients present with advanced stages. although polysaccharide peptides (psp), isolated from the fungus coriolus versicolor, have been reported to have anti-tumor effects, its clinical efficacy has not been properly evaluated. | 2003 | 12814145 |
| enhanced decolourisation ability of laccase towards various synthetic dyes by an electrocatalysis technology. | laccase in culture filtrates of trametes versicolor degraded a number of structurally different dyes by about 30% after 30 min though only 5% of azure b was degraded in 1 h and poly r-478 and fuchsin were not degraded at all even after 24 h. however, by using enzymatic electrocatalysis technology all dyes were decolourised in about 30 to 135 min. | 2003 | 12882152 |
| a screening method for detecting iron reducing wood-rot fungi. | a plate assay using the fe(ii) selective dye, ferrozine, for detecting wood-rot fungi with fe(iii) reductive abilities, was developed. the assay is fast, simple and, in most cases, more sensitive than the corresponding liquid medium test. the brown rot fungi, gloeophyllum trabeum and laetiporeus sulphureus, displayed higher iron reductive capabilities than white rot fungi, trametes versicolor, ganoderma australe and ceriporiopsis subvermispora. | 2003 | 12889801 |
| bioorganosolve pretreatments for simultaneous saccharification and fermentation of beech wood by ethanolysis and white rot fungi. | ethanol was produced by simultaneous saccharification and fermentation (ssf) from beech wood chips after bioorganosolve pretreatments by ethanolysis and white rot fungi, ceriporiopsis subvermispora, dichomitus squalens, pleurotus ostreatus, and coriolus versicolor. beech wood chips were pretreated with the white rot fungi for 2-8 weeks without addition of any nutrients. the wood chips were then subjected to ethanolysis to separate them into pulp and soluble fractions (sfs). from the pulp fractio ... | 2003 | 12890613 |
| identification and functional expression of ctaa, a p-type atpase gene involved in copper trafficking in trametes versicolor. | here the identification and characterization of a gene encoding a copper-trafficking enzyme, ctaa (copper-transporting atpase), from the basidiomycete trametes versicolor are described. this p-type copper atpase gene has two alleles, differing primarily in the length of the second, unusually long intron, and encodes a 983 aa protein with 40 % sequence identity to yeast ccc2p. overexpression of ctaa in yeast grown in the presence of copper led to a 15-fold increase in laccase yields, while overex ... | 2003 | 12904544 |
| effect of nonylphenol surfactants on fungi following the application of sewage sludge on agricultural soils. | the effect of nonylphenol on fungi following the application of contaminated sewage sludge on agricultural soil was studied in laboratory experiments. nonylphenol bioavailability and adsorption were determined in the soil alone and soil-sludge mixtures. mixing the soil with sludge made it possible to measure the nonylphenol concentration in the soil solution, which comprised between 6.6 x 10(-6) and 3.8 x 10(-7) m, according to the sludge. we then examined the dose-response relationship between ... | 2003 | 12931882 |
| biosorption of heavy metal ions on immobilized white-rot fungus trametes versicolor. | trametes versicolor mycelia were immobilized in carboxymethylcellulose, cmc, beads via entrapment, and the bead containing immobilized fungus spores were incubated at 30 degrees c for 3 days to attain uniform growth on the bead surface. after incubation, the live and heat inactivated immobilized fungus on the cmc beads were used for the biosorption of cu(2+), pb(2+) and zn(2+) ions. plain cmc beads were used as a control system. the biosorption of cu(2+), pb(2+) and zn(2+) ions by the cmc and bo ... | 2003 | 12935760 |
| polysaccharopeptides of coriolus versicolor: physiological activity, uses, and production. | the protein-bound polysaccharides or polysaccharopeptides produced by coriolus versicolor are effective immunopotentiators, which are used to supplement the chemotherapy and radiotherapy of cancers and various infectious diseases. antitumor activity of polysaccharopeptides has been documented. several kinds of protein-bound polysaccharides have been shown to be produced by the white rot fungus, c. versicolor. although some of these polymers are structurally distinct, they are not distinguishable ... | 2003 | 14499133 |
| decolorization and bioremediation of molasses wastewater by white-rot fungi in a semi-solid-state condition. | molasses wastewater (vinasse; the by-product of distillation of fermented sugar) was decolorized and its chemical oxygen demand (cod) was reduced in static cultivation using the fungi coriolus versicolor, funalia trogii, phanerochaete chrysosporium and pleurotus pulmonarius ('pleurotus sajorcaju'). the effect of cotton stalk on decolorizing and cod removing capability of four fungi was determined. in the entire concentration range tested (10-30%), wastewater was effectively decolorized by c. ver ... | 2003 | 14533485 |
| copper dissociation as a mechanism of fungal laccase denaturation by humic acid. | effects of humic acid on removal of hydroxy polychlorobiphenyls (pcbs) with laccase from trametes versicolor were studied. in the absence of humic acid, hydroxy pcbs were rapidly degraded by laccase. however, the rate constants decreased with increasing humic acid concentration, the reactions being completely inhibited at 150 mg l(-1) of humic acid. peroxidase from arthromyces ramosus was not inhibited by the same treatment. the activity of humic acid-deactivated laccase was completely restored ... | 2004 | 14564487 |
| colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases. | polycyclic aromatic hydrocarbons (pahs) are highly toxic organic pollutants widely distributed in terrestrial and aquatic environments. in the present work, 2 colorimetric assays for laccase-catalyzed degradation of pahs were developed based on studies of the oxidation of 12 aromatic hydrocarbons by fungal laccases from trametes versicolor and myceliophthora thermophila. using a sodium borohydride water-soluble solution, the authors could reduce the single product of laccase-catalyzed anthracene ... | 2002 | 14599353 |
| biotransformation of n-(2-alkylamino-4-phenylimidazol-1-yl)-acetamides and kinetic studies by using cells and laccase from trametes versicolor. | the product pattern of biotransformation of different n-(2-alkylamino-4-phenylimidazol-1-yl)-acetamides were compared. n-(2-alkylamino-4-phenylimidazol-1-yl)-acetamides were transformed by trametes versicolor into two types of aromatic products identified by chemical structure analysis: 1. novel heteroaromatic compounds, such as: 2-(alkylamino)-4-phenylimidazoles, n-(2-alkylamino-5-formyl-4-phenylimidazol-1-yl)-acetamides, n-(2-alkylimino-3-hydroxy-4-phenyl-2,3-dihydroimidazol-1-yl)-acetamides a ... | 2003 | 14625901 |
| discrimination against 13c during degradation of simple and complex substrates by two white rot fungi. | changes in isotopic 13c signatures of co2-c evolved during decomposition of a sugar (glucose), a fatty acid (palmitic acid), a protein (albumin), a structural biopolymer (lignin) and bulk plant tissue (aerial shoots from lolium perenne) were monitored over a period of 76 days. all materials were sterilized and inoculated with either of two different species of white rot fungi, phanerochaete chrysosporium or coriolus versicolor, and incubated in sealed bottles at 28 degrees c. the co2 concentrati ... | 2003 | 14648898 |
| improvement of foreign-protein production in aspergillus niger var. awamori by constitutive induction of the unfolded-protein response. | unfolded-protein response (upr) denotes the upregulation of endoplasmic reticulum (er)-resident chaperone and foldase genes and numerous other genes involved in secretory functions during the accumulation of unfolded proteins into the er. overexpression of individual foldases and chaperones has been used in attempts to improve protein production in different production systems. we describe here a novel strategy to improve foreign-protein production. we show that the constitutive induction of the ... | 2003 | 14660339 |
| a quantitative structure-antifungal activity relationship study of oxygenated aromatic essential oil compounds using data structuring and pls regression analysis. | twenty two oxygenated aromatic essential oil compounds were chosen for the study of the antifungal activity against two wood-decaying fungi, the white-rot trametes versicolor, which mainly metabolizes lignin, and the brown-rot coniophoha puteana, which digests cellulose in plant cell walls. minimal inhibitory concentrations (mics) were determined by the agar dilution method, using dimethyl sulfoxide (dmso) as the solvent for the selected compounds and potato-dextrose agar (pda) as the growth med ... | 2004 | 14689256 |
| purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete, tricholoma matsutake. | a new h(2)o(2)-generating pyranose oxidase was purified as a strong antifungal protein from an arbuscular mycorrhizal fungus, tricholoma matsutake. the protein showed a molecular mass of 250 kda in gel filtration, and probably consisted of four identical 62 kda subunits. the protein contained flavin moiety and it oxidized d-glucose at position c-2. h(2)o(2) and d-glucosone produced by the pyranose oxidase reaction showed antifungal activity, suggesting these compounds were the molecular basis of ... | 2003 | 14730138 |
| biologically active compounds from aphyllophorales (polypore) fungi. | this review describes biologically active natural products isolated from aphyllophorales, many of which are known as polypores. polypores are a large group of terrestrial fungi of the phylum basdiomycota (basidiomycetes), and they along with certain ascomycota are a major source of pharmacologically active substances. there are about 25 000 species of basidiomycetes, of which about 500 are members of the aphyllophorales, a polyphyletic group that contains the polypores. many of these fungi have ... | 2004 | 14987072 |
| immunomodulatory effects of yun zhi and danshen capsules in health subjects--a randomized, double-blind, placebo-controlled, crossover study. | immunostimulating polysaccharides extracted from the chinese medicinal plant yun zhi (coriolus versicolor) have been found to enhance various immunological functions, and danshen (salvia miltiorrhiza) to show beneficial effects on the circulatory system. in the present clinical study, we investigated if regular consumption of yun zhi and danshen capsules could improve cellular immunity in healthy subjects. one hundred healthy subjects were recruited to take yun zhi (50 mg/kg body weight) plus da ... | 2004 | 14996412 |
| effect of psk on the maturation of dendritic cells derived from human peripheral blood monocytes. | dendritic cells (dcs) are powerful antigen-presenting cells (apcs) that have attracted attention in recent years from the viewpoint of dc vaccine therapy against cancer. however, the existence of a strongly immunosuppressed state in cancer-bearing individuals inhibits dc maturation, which is one of the problems facing anti-cancer dc vaccine therapy. protein-bound polysaccharide k (psk), which is extracted from the cultured mycelium of coriolus versicolor (fr.) quél, is used as an anti-cancer age ... | 2004 | 15019294 |
| decoloration of a carpet dye effluent using trametes versicolor. | although a non-sterile, undiluted carpet dye effluent (containing two anthraquinone dyes) did not support growth of trametes versicolor, the pre-grown fungus removed 95% of its color in shake-flasks after 10 h of incubation. after decoloration, the cod of the cell-free supernatant increased and the toxicity was unchanged as determined by the microtox assay using vibrio fischeri. decoloration rates decreased when either glucose alone or mn2+ and glucose were added. t. versicolor, immobilized on j ... | 2004 | 15049362 |
| real-time pcr analysis of carbon catabolite repression of cellobiose dehydrogenase gene transcription in trametes versicolor. | cellobiose dehydrogenase production in trametes versicolor is repressed when additional carbon sources, such as glucose, maltose, galactose, arabinose, and xylose, are added to the fungal cultures growing on cellulose. real-time quantitative reverse transcription-polymerase chain reaction has been used to demonstrate that the addition of galactose, arabinose, and xylose results in 19-, 92-, and 114-fold reductions, respectively, in cdh transcript levels 96 h post-addition. glucose exhibits the g ... | 2004 | 15052313 |
| different proportions of laccase isoenzymes produced by submerged cultures of trametes versicolor grown on lignocellulosic wastes. | the white-rot fungus trametes versicolor grown in submerged culture produced two laccase isoenzymes, laci and lacii. addition of insoluble lignocellulosic materials into the culture medium increased the total laccase activity. the proportion of laccase isoenzymes also changed depending on the lignocellulosic material employed, with ratios of activity lacii/laci from 0.9 (barley straw) to 4.4 (grape stalks). besides, this proportion played an important role in the dye decolourisation. | 2004 | 15055770 |
| biodecolorization of azo, anthraquinonic and triphenylmethane dyes by white-rot fungi and a laccase-secreting engineered strain. | one laccase-secreting engineered strain and four white-rot fungi were tested for their capacity to decolorize nine dyes that could be classified as azo, anthraquinonic and triphenylmethane dyes. trametes versicolor was the most efficient of the tested strains under these experimental conditions. anthraquinonic dyes were decolorized more easily than the other two types. small structural differences among the dyes could significantly affect decolorization. none of the strains showed lignin peroxid ... | 2004 | 15069603 |
| mechanism of textile metal dye biotransformation by trametes versicolor. | the biodegradation of grey lanaset g, which consists of a mixture of metal complexed dye, was studied. experiments were carried out in a bioreactor with retained pellets of the fungus trametes versicolor that was operated under conditions of laccase production. although decolorization was highly efficient (90%), no direct relationship to extracellular enzyme was apparent. moreover, the extracellular enzyme was found to be unable to degrade the dye in vitro. the process involves several steps. th ... | 2004 | 15087198 |
| fungal laccase-catalyzed degradation of hydroxy polychlorinated biphenyls. | hydroxy polychlorinated biphenyls (hydroxy pcbs) are toxic metabolites of pcbs. their toxicity such as strong endocrine disruption demands effective remediation methods. laccases from trametes versicolor and pleurotus ostreatus were tested to degrade hydroxy pcbs. optimum phs for both enzymes were around 4.0. laccase from t. versicolor degrades hydroxy pcbs more rapidly than that from p. ostreatus. the enzymatic activities remained little changes in up to 10% organic solvents, but decreased rapi ... | 2004 | 15109876 |
| sequence polymorphism and molecular characterization of laccase genes of the conifer pathogen heterobasidion annosum. | the oxidizing enzyme laccase produced by many fungi is generally considered to be active in the biodegradation of lignin, a major plant cell wall component highly resistant to microbial attack. the enzyme is secreted at high levels by the p-type of the highly aggressive pathogen heterobasidion annosum, but at much lower levels by the s-type which correlated with their varying wood decay capability. to investigate the evolutionary relationship between laccase genes of the different h. annosum typ ... | 2004 | 15119350 |
| evaluation of argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes. | the decolorizing capacity of 26 white rot fungi from argentina was investigated. extracellular production of ligninolytic enzymes by mycelium growing on solid malt extract/glucose medium supplemented with different dyes (malachite green, azure b, poly r-478, anthraquinone blue, congo red and xylidine), dye decolorization and the relationship between these two processes were studied. only ten strains decolorized all the dyes, all ten strains produced laccase, lignin peroxidase and manganese perox ... | 2004 | 15158509 |
| cytotoxic activities of coriolus versicolor (yunzhi) extract on human leukemia and lymphoma cells by induction of apoptosis. | coriolus versicolor (cv), also known as yunzhi, is one of the commonly used chinese medicinal herbs. although recent studies have demonstrated its antitumour activities on cancer cells in vitro and in vivo, the exact mechanism is not fully elucidated. hence, the objective of this study was to examine the in vitro cytotoxic activities of a standardized aqueous ethanol extract prepared from coriolus versicolor on a b-cell lymphoma (raji) and two human promyelocytic leukemia (hl-60, nb-4) cell line ... | 2004 | 15183073 |
| preliminary evaluation of fungicidal and termiticidal activities of filtrates from biomass slurry fuel production. | biomass slurry fuel (bsf) production has recently been developed as a natural energy for the conversion of solid biomass into fuel. in addition to using fuel, filtrates from bsf production may also serve a chemical source with several organic compounds. there is an increasing interest in the research and application of biomass-based filtrates. in this study, fungicidal and termiticidal properties of filtrates from bsf production using sugi (cryptomeria japonica) and acacia (acacia mangium) wood ... | 2004 | 15207293 |
| growth substrate selection and biodegradation of pcp by new zealand white-rot fungi. | nine new zealand native white-rot fungi were studied for their ability to grow and survive on different substrates formulated from bark, wheat straw, sawdust, apple pomace and maize products in order to identify their pentachlorophenol (pcp) biodegradation potential and to select a fungal carrier for bioaugmentation of polluted soils. isolates were also evaluated to mineralize (14)c-pcp in liquid culture and in soil. the american fungus phanerochaete chrysosporium outgrew the native fungi on the ... | 2004 | 15217724 |
| fungal polysaccharopeptide inhibits tumor angiogenesis and tumor growth in mice. | angiogenesis is crucial to tumor growth and metastasis, and interruption of this process is a prime avenue for therapeutic intervention of tumor proliferation. the present study has made use of the s180 tumor-bearing mouse model to investigate the polysaccharopeptide, psp, isolated from the edible mushroom coriolus versicolor, a herbal medicine known for its anti-angiogenesis properties. quantitative analysis of microcorrosion casting of the tumor tissue showed more angiogenic features such as d ... | 2004 | 15234192 |
| degradation of acrylic copolymers by white-rot fungi. | various water-soluble homopolymers and copolymers of acrylamide (aam) and acrylic acid (aa) which contained phenolic sites, such as guaiacol, lignin sulfonate (ls) and 3,4-dihydroxybenzoic acid (3,4-dhba), were tested with regard to their degradability by white-rot fungi. compared with phanerochaete chrysosporium, pleurotus ostreatus caused a significantly higher decrease in the average molecular weight ( mw) of most of the copolymers and the homopolymer under the applied culture conditions. how ... | 2004 | 15257422 |
| treatment of cancer with mushroom products. | cancer has been attributed to 3 causes: pollution, infection, and poor nutrition. conventional treatments include surgery, chemotherapy, and radiotherapy. the author proposes that immunotherapy also be considered. among other environmental influences, dietary deficiencies and carcinogenic viral infections must be investigated and treated wherever possible. it has been suggested that mushrooms, in particular, have a structure that is immunomodulatory because it resembles the proteoglycan structur ... | 2003 | 15259434 |
| degradation of toluene and benzene by trametes versicolor. | in this study, biological degradation of non-polar monoaromatic compounds, benzene and toluene, by one of the white rot fungi, namely trametes versicolor was analyzed and the biomass formed was determined. the studies were carried out in mediums which contain basic nutrients in certain amounts, toluene and benzene at concentrations of 50, 100, 200, 250 and 350 mg/l, ph at 5, temperature at 28 degrees c and rpm at 150. within an incubation period of 48 hours, it was observed that, removal was com ... | 2004 | 15303700 |
| laccase from trametes versicolor: stability at temperature and alkaline conditions and its effect on biobleaching of hardwood kraft pulp. | the enzyme laccase was produced by the white-rot fungus trametes versicolor in repeated batches cultures with immobilized mycelium. two different culture conditions were used. enzymes produced were evaluated regarding their stability at high temperatures (55 degrees c and 65 degrees c) and at alkaline conditions (ph 7.0 and ph 8.0) having in view the application of these enzymes in biobleaching of hardwood kraft pulp. biobleaching experiments were divided in two parts, enzymatic prebleaching fol ... | 1999 | 15304692 |
| bioremediation of paper and pulp mill effluents. | pulp and paper mill effluents pollute water, air and soil, causing a major threat to the environment. several methods have been attempted by various researchers throughout the world for the removal of colour from pulp and paper mill effluents. the biological colour removal process uses several classes of microorganisms--bacteria, algae and fungi--to degrade the polymeric lignin derived chromophoric material. white rot fungi such as phanerochaete chrysosporium, corius versicolor, trametes versico ... | 2003 | 15332490 |
| expression of laccase iiib from the white-rot fungus trametes versicolor in the yeast yarrowia lipolytica for environmental applications. | improvement of the catalytic properties of fungal laccases is a current challenge for the efficient bioremediation of natural media polluted by xenobiotics. we developed the heterologous expression of a laccase from the white-rot fungus trametes versicolor in the yeast yarrowia lipolytica as a first step for enzyme evolution. the full-length cdna consisted of a 1,561-bp open reading frame encoding laciiib, a 499-amino-acid protein and a 21-amino-acid signal peptide. native and yeast secretion si ... | 2005 | 15349699 |
| differential effect of coriolus versicolor (yunzhi) extract on cytokine production by murine lymphocytes in vitro. | being one of the commonly used chinese medicinal herbs, coriolus versicolor (cv), also named as yunzhi, was known to possess both anti-tumor and immunopotentiating activities. the present study aimed to investigate the in vitro immunomodulatory effect of a standardized ethanol-water extract prepared from cv on the proliferation of murine splenic lymphocytes using the mtt assay, and the production of six t helper (th)-related cytokines using the enzyme-linked immunosorbent assay (elisa) technique ... | 2004 | 15351324 |
| degradation of phenanthrene by trametes versicolor and its laccase. | phenanthrene is a three-ring polycyclic aromatic hydrocarbon and commonly found as a pollutant in various environments. degradation of phenanthrene by white rot fungus trametes versicolor 951022 and its laccase, isolated in korea, was investigated. after 36 h of incubation, about 46% and 65% of 100 mg/l of phenanthrene added in shaken and static fungal cultures were removed, respectively. phenanthrene degradation was maximal at ph 6 and the optimal temperature for phenanthrene removal was 30 deg ... | 2004 | 15357301 |
| integrated biological treatment of recalcitrant effluents from pulp mills. | this work aimed at determining the degree of depuration of a recalcitrant effluent (weak black liquor, wbl) achieved in a series treatment consisting of a first stage methanogenic fluidised bed reactor followed by a second stage aerobic, upflow reactor packed with "biocubes" of trametes versicolor immobilised onto small cubes of holm oak wood. the mesophilic, lab scale methanogenic fluidised bed reactor contained a microbial consortium immobilised onto granular activated carbon 500 microm averag ... | 2004 | 15461409 |
| laccase-catalyzed mediated oxidation of benzyl alcohol: the role of tempo and formation of products including benzonitrile studied by nanoelectrospray ionization fourier transform ion cyclotron resonance mass spectrometry. | substituted benzyl alcohol was oxidized enzymatically with a laccase-mediator system and the products were investigated as a function of time by nanoelectrospray ionization fourier transform ion cyclotron resonance mass spectrometry (nanoesi-fticrms). with trametes versicolor laccase (tvl), the mediator, 2,2',6,6'-tetramethylpiperidine-n-oxyl radical (tempo), undergoes oxidation and forms oxoammonium ion. oxidized tempo oxidizes the alcohol and is simultaneously reduced to the n-oh form. the lac ... | 2004 | 15468136 |
| decolorization and degradation of xanthene dyes by a white rot fungus, coriolus versicolor. | the decolorization of six xanthene dyes (conc. 100 microm) by a white rot fungus, coriolus versicolor (c. versicolor), was investigated in liquid culture. the decolorization of fluorescein, 4-aminofluorescein, and 5-aminofluorescein by the fungus was 85.0, 95.0, and 91.9% after 14 days incubation, respectively. however, no decolorization of rhodamine b, rhodamine 123 hydrate, and rhodamine 6g was observed. the first three dyes also were decolorized with cell-free extracts from c. versicolor. the ... | 2004 | 15478930 |
| overproduction of recombinant laccase using a homologous expression system in coriolus versicolor. | one of the major extracellular enzymes of the white-rot fungus coriolus versicolor is laccase, which is involved in the degradation of lignin. we constructed a homologous system for the expression of a gene for laccase iii (cvl3) in c. versicolor, using a chimeric laccase gene driven by the promoter of a gene for glyceraldehyde-3-phosphate dehydrogenase (gpd) from this fungus. we transformed c. versicolor successfully by introducing both a gene for hygromycin b phosphotransferase (hph) and the c ... | 2004 | 15480638 |
| the effect of nitrogen supplementation on the efficiency of colour and cod removal by malaysian white-rot fungi in textile dyeing effluent. | white-rot fungi, namely coriolus versicolor and schizophyllum commune, were studied for the biodecolorization of textile dyeing effluent in shaker-flask experiments. the results showed that c. versicolor was able to achieve 68% color removal after 5 days of treatment while that of s. commune was 88% in 9 days. both fungi achieved the above results in non-sterile condition with diammonium hydrogen phosphate as the nutrient supplement. on the other hand, the best cod removal of 80% was obtained wi ... | 2004 | 15497832 |
| transgenic tobacco expressing fungal laccase promotes the detoxification of environmental pollutants. | the phytoremediation of soils contaminated with organic pollutants offers a low-cost method for removal of such pollutants. we have attempted to enhance the environmental decontamination functions of plants by introducing appropriate enzymatic activities from microorganisms. in the present study, we introduced an extracellular fungal enzyme, the laccase of coriolus versicolor, into tobacco plants. one transgenic plant, designated fl4, produced laccase that was secreted into the rhizosphere. fl4 ... | 2005 | 15549288 |