| in vivo and in vitro modulation of nk and adcc activities of mouse spleen cells by peptidoglycan monomer (pgm). | the ability of peptidoglycan monomer (pgm), an immunomodulator obtained from brevibacterium divaricatum, to modulate nk and adcc activities of spleen cells was tested in mice with constitutively weak (c57b1) or strong nk activity (cba, c3h). in weak reactors, i.v. injection of pgm enhanced the nk and adcc activity as effectively as a known stimulator, poly i.c, and the dynamics of the response was comparable. in strong reactors, pgm caused two peaks of the adcc response, and one peak of nk stimu ... | 1989 | 2813963 |
| nitrile hydratase is a quinoprotein. a possible new function of pyrroloquinoline quinone: activation of h2o in an enzymatic hydration reaction. | nitrile hydratase has been proved to be a quinoprotein with pyrroloquinoline quinone (pqq) as a prosthetic group. the broad shoulder from 300 to 500 nm in the absorption spectrum of brevibacterium nitrile hydratase suggested the presence of pqq. since pqq was attached to the enzyme through a covalent linkage, the chromophores were isolated by acid hydrolysis, protease digestion and successive chromatographic separation. the isolated chromophores showed the similar spectroscopic characteristics t ... | 1987 | 2820412 |
| sequence analysis of the brevibacterium lactofermentum trp operon. | brevibacterium lactofermentum, a gram-positive bacterium, is a commercially important amino acid producer. in this organism, the tryptophan biosynthetic enzymes are encoded within a 7725 bp hapii-bamhi fragment. seven open reading frames were identified as trp genes by complementation tests with various b. lactofermentum and escherichia coli tryptophan auxotrophs. following the nomenclature established for e. coli and serratia marcescens, the b. lactofermentum trp genes were designated trpl, trp ... | 1987 | 2823076 |
| ribonucleotide reductase of brevibacterium ammoniagenes is a manganese enzyme. | ribonucleotide reduction and not dna replication is the site for the specific manganese requirement of dna synthesis and cell growth in the coryneform bacterium brevibacterium ammoniagenes. to characterize the metal effect we have isolated and purified ribonucleoside-diphosphate reductase from overproducing bacteria that were first deprived of and then reactivated by manganese ions. purification on columns of sephacryl s400, deae-cellulose and hydroxyapatite provided an apparently homogeneous en ... | 1988 | 2828045 |
| bepi restriction endonuclease, a new isoschisomer of fnudii. | | 1988 | 2829121 |
| significant interaction between low-spin iron(iii) site and pyrroloquinoline quinone in active center of nitrile hydratase. | nitrile hydratase, a unique non-heme iron enzyme, contains low-spin fe(iii) site and pyrroloquinoline quinone in the active center. the enzymatic activity of nitrile hydratase is strongly inhibited by typical carbonyl reagents such as phenylhydrazine and semicarbazide. of special interest is the fact that these carbonyl reagents induced significant alteration of the esr features of the native iron(iii) enzyme at ph 7.2. in addition, the isobutyronitrile(inhibitor)-bound enzyme was also remarkabl ... | 1988 | 2840901 |
| a host-vector system for an arthrobacter species. | an efficient host-vector system has been developed for an industrial strain of arthrobacter sp. (nrrl b3728)used for glucose isomerase production. protoplasts of arthrobacter were generated by treating the cells with 0.5 mg lysozyme ml(-1) for 60 min in a solution containing 0.5 m-sucrose. around 30% of the protoplasts regenerated on agar containing 0.5 m-sodium succinate as osmotic stabilizer. three hybrid vectors, pbl2100, pcg1100 and pcg2100, were constructed by combining the escherichia coli ... | 1988 | 2846755 |
| troubles underfoot. | | 1985 | 2861531 |
| cloning and expression in escherichia coli of genes involved in the lysine pathway of brevibacterium lactofermentum. | the brevibacterium lactofermentum genes which complement escherichia coli lysa and asd-1 mutants were identified, respectively, as a 1.9-kilobase psti-clai fragment and a 2.5-kilobase psti fragment by cloning into pbr325. southern blot transfers show hybridization to chromosomal fragments of identical size. the putative b. lactofermentum asd and lysa products are 44 and 48 kilodaltons, respectively. | 1985 | 2864331 |
| a rapid assay method for ammonia using glutamine synthetase from glutamate-producing bacteria. | a rapid enzymatic assay method for ammonia was developed by using glutamine synthetase from glutamate-producing bacteria together with pyruvate kinase, lactate dehydrogenase, and nadh. the time required for determination of 25 nmol of ammonia was 5 min with 1 unit of glutamine synthetase, as opposed to 14-30 min with 1 unit of glutamate dehydrogenases from various sources. the present method was used to determine ammonia in serum, microbiol-culture broth, and waste water. the method can be modif ... | 1987 | 2887129 |
| comparative susceptibility of a peptidoglycan monomer from brevibacterium divaricatum and its anhydromuramyl analogue to hydrolysis with n-acetylmuramyl-l-alanine amidase. isolation and characterization of anhydromuramyl-peptidoglycan monomer. | peptidoglycan monomer, glcnac-beta-(1----4)-murnac-l-ala-d-igln[ (l)-meso-a2pm-(d)-amide-(l)-d-ala-d-ala] (pgm), from brevibacterium divaricatum is composed of the disaccharide pentapeptide containing muramic acid with a reducing end (ca. 90-95%) and of the anhydromuramyl analogue (anhydromuranyl-pgm; ca. 5-10%), according to analysis by high-performance liquid chromatography (hplc) and fast atom bombardment mass spectrometry (fab-ms). the two peptidoglycan analogues cannot be separated by simpl ... | 1988 | 2900248 |
| cloning of the lambda resistant genes from brevibacterium albidum and proteus vulgaris into escherichia coli. | genes from proteus vulgaris atcc13315 and brevibacterium albidum atcc15831 were introduced into escherichia coli, which rendered the host resistant to coliphage lambda. the clones transformed by any one of the two recombinant plasmids, prmg101 or prmg216, were totally resistant against the infection of virulent lambda and n4, but sensitive to ø80, t4 and t7. however, when maltose transport systems of the clones were induced by maltose, the clones were no more resistant to the phage: thus, this p ... | 1986 | 2946296 |
| natural-abundance 13c nuclear magnetic resonance studies of regulation and overproduction of l-lysine by brevibacterium flavum. | natural-abundance 13c nmr spectroscopy has been used to study the metabolism of the l-lysine-producing bacterium, brevibacterium flavum. relationships of biomass formation, precursor uptake, and product excretion, as a function of culture medium, oxygen supply and specific cell membrane permeability, were rapidly determined using 67.89-mhz 13c nmr. the induction of lysine production throughout the growth cycle was studied. intracellular and extracellular levels of free metabolites and unconsumed ... | 1985 | 2988953 |
| determination of the complete nucleotide sequence of the brevibacterium lactofermentum plasmid pam330 and the analysis of its genetic information. | the complete nucleotide sequence of the plasmid pam330 derived from brevibacterium lactofermentum atcc 13869 was determined using the dideoxy chain termination method. analysis of the sequence allowed us to observe seven open reading frames, one of which showed the presence of the promoter and the shine-dalgarno (sd) sequences upstream from the initiation codon. | 1985 | 3003705 |
| protoplast transformation in coryneform bacteria and introduction of an alpha-amylase gene from bacillus amyloliquefaciens into brevibacterium lactofermentum. | the goal of this study was to investigate the likelihood of developing useful transformation systems for coryneform bacteria. two species of coryneform bacteria, brevibacterium lactofermentum and corynebacterium lilium, were transformed with chimeras constructed from pub110 and a cryptic coryneform plasmid (pgx1901). c. lilium protoplasts were also efficiently transfected with phage cs1 dna. high transformation and transfection frequencies were obtained after only 2 min of lysozyme treatment of ... | 1986 | 3008649 |
| purification and characterization of fad synthetase from brevibacterium ammoniagenes. | the bifunctional enzyme fad synthetase from brevibacterium ammoniagenes was purified by a method involving atp-affinity chromatography. the final preparation was more than 95% pure. the apparent molecular weight of the enzyme was determined as 38,000 and the isoelectric point as 4.6. although previous attempts to separate the enzymatic activities had failed, atp:riboflavin 5'-phosphotransferase and atp:fmn-adenylyltransferase activities in b. ammoniagenes were believed to be located on two separ ... | 1986 | 3023344 |
| 13c-nmr, 1h-nmr and gas-chromatography mass-spectrometry studies of the biosynthesis of 13c-enriched l-lysine by brevibacterium flavum. | the basic metabolic pathways of lysine biosynthesis in brevibacterium flavum, a strain which excretes excessive amounts of l-lysine, have been followed by using two 13c-labeled precursors. 13c- and 1h-nmr spectroscopies in conjunction with gas chromatography mass spectrometry (gc-ms) have revealed the various metabolic pathways leading to l-[13c]lysine. discrete metabolic pathways give rise to distinct labeling patterns. l-lysine resulting from [1-13c]glucose fermentation is relatively specifica ... | 1987 | 3030742 |
| structural characteristics of the corynebacterium lilium bacteriophage cl31. | bacteriophage cl31 was isolated on a corynebacterium lilium strain. out of 30 strains tested, only cl31 was able to form plaques on corynebacterium glutamicum atcc 13287, brevibacterium lactofermentum atcc 21086, and arthrobacter sp. strain si55, but at a very low frequency. this phage belongs to group b of bradley's classification (d. e. bradley, bacteriol. rev. 31:230-314; 1967). its head is 53 nm in diameter, and its tail is 396 nm in length. the phage capsid contains three major proteins, of ... | 1987 | 3033280 |
| cloning and expression in escherichia coli of the homoserine kinase (thrb) gene from brevibacterium lactofermentum. | five dna fragments carrying the thrb gene (homoserine kinase e.c. 2.7.1.39) of brevibacterium lactofermentum were cloned by complementation of escherichia coli thrb mutants using pbr322 as vector. all the cloned fragments contained a common 3.1 kb dna sequence. the cloned fragments hybridized among themselves and with a 9 kb bamhi fragment of the chromosomal dna of b. lactofermentum but not with the dna of e. coli. none of the cloned fragments were able to complement thra and thrc mutations of e ... | 1987 | 3035340 |
| nucleotide sequence of the homoserine kinase (thr b) gene of brevibacterium lactofermentum. | | 1987 | 3035505 |
| nucleotide and thioredoxin specificity of the manganese ribonucleotide reductase from brevibacterium ammoniagenes. | the manganese-containing ribonucleotide reductase previously identified in gram-positive bacteria has been purified and its nucleotide specificity and other requirements were determined. the enzyme isolated from brevibacterium ammoniagenes is a ribonucleoside-diphosphate reductase which, in the presence of allosteric effectors, reduces all four common substrates at comparable rates; very little activity is observed in the absence of effector nucleotides. ribonucleoside triphosphates are reduced ... | 1988 | 3042394 |
| automated continuous crosscurrent extraction of proteins. | | 1988 | 3067630 |
| biosynthetic preparation of l-[13c]- and [15n]glutamate by brevibacterium flavum. | the biosynthesis of isotopically labeled l-glutamic acid by the microorganism brevibacterium flavum was studied with a variety of carbon-13-enriched precursors. the purpose of this study was twofold: to develop techniques for the efficient preparation of labeled l-glutamate with a variety of useful labeling patterns which can be used for other metabolic studies, and to better understand the metabolic events leading to label scrambling in these strains. b. flavum, which is used commercially for t ... | 1987 | 3103536 |
| phylogenetic analysis of the coryneform bacteria by 5s rrna sequences. | nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ... | 1987 | 3106318 |
| the amidases from a brevibacterium strain: study and applications. | | 1988 | 3142225 |
| cloning of the trp genes from the archaebacterium methanococcus voltae: nucleotide sequence of the trpba genes. | a cosmid bank of methanococcus voltae dna was obtained in escherichia coli after ligation of partially hindiii-digested m. voltae dna in the hindiii site of the transferable cosmid pvk100. the bank was used to perform complementation experiments with e. coli auxotrophic mutants. five cosmids complementing trpa shared three adjacent hindiii fragments of 2.1, 2.3 and 14 kb. two of these cosmids also complemented trpd and carried an additional 4.2 kb hindiii fragment. the trpa- and trpd- complement ... | 1988 | 3146017 |
| nucleotide sequence of the threonine synthase (thrc) gene of brevibacterium lactofermentum. | | 1988 | 3186450 |
| nucleotide sequence of the homoserine dehydrogenase (thr a) gene of brevibacterium lactofermentum. | | 1987 | 3320973 |
| pigmentation changes in brevibacteria induced by mutagenic treatment. | inducible pigmentation changes were observed in pigmented strains of brevibacterium sp. m27 and b. flavum treated with n-methyl-n'-nitro-n-nitrosoguanidine. the highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. as compared with the initial yellow colour, three new pigmentation types, viz. white, pink and orange, were observed. the yellow pigmented parent strains are most resistant to the lethal effects of uv radiation. by selecting pigmen ... | 1988 | 3360380 |
| a peptidoglycan monomer as an antitumor agent in mice: stimulation of phagocytosis by resident peritoneal macrophages with peptidoglycan monomer. | peptidoglycan monomer (dissacharide-pentapeptide, pgm) from brevibacterium divaricatum is an immunomodulator and an antitumor agent. as part of our investigation of the antitumor properties of pgm in mice, its potential to stimulate phagocytosis by resident peritoneal macrophages was assessed. inbred cba/h/zg tau mice (kept under conventional conditions) were used, and a simple and brief method was developed to evaluate phagocytosis. it consisted of a short (10 min) coincubation of yeast cells ( ... | 1988 | 3373235 |
| occurrence of a cobalt-induced and cobalt-containing nitrile hydratase in rhodococcus rhodochrous j1. | the formation of nitrile hydratase required cobalt ions in rhodococcus rhodochrous j1. no other transition-metals could replace the cobalt ion. the rhodococcus nitrile hydratase was purified to homogeneity and found to contain a cobalt atom. the occurrence of a cobalt-induced and cobalt-containing nitrile hydratase, different from the nitrile hydratases in pseudomonas chlororaphis b23 and brevibacterium r312 containing a ferric ion in their active center, has been demonstrated here for the first ... | 1988 | 3421954 |
| the expression of cellulomonas fimi cellulase genes in brevibacterium lactofermentum. | the exoglucanase gene (cex) and the endoglucanase a gene (cena) from cellulomonas fimi were subcloned into the escherichia coli/brevibacterium lactofermentum shuttle vector pbk10. both genes were expressed to five to ten times higher levels in b. lactofermentum than in e. coli, probably because these genes were expressed from c. fimi promoters. in b. lactofermentum virtually all of the enzyme activities were in the culture supernatant. this system will facilitate analysis of the expression of th ... | 1987 | 3443308 |
| active site organization of bacterial type i fatty acid synthetase. | four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respect to specific labels by sds-polyacrylamide gel electrophoresis and a fluorographic technique. the linear alignment of such fragments in the original subunit was established and whe ... | 1987 | 3448090 |
| complete nucleotide sequence of a native plasmid of brevibacterium lactofermentum. | | 1986 | 3453107 |
| identification of a promoter sequence in the plasmid pul340 of brevibacterium lactofermentum and construction of new cloning vectors for corynebacteria containing two selectable markers. | a strong promoter p1 has been found in plasmid pul340, a cloning vector used to transform corynebacteria. this promoter is also expressed efficiently in escherichia coli. a gene (cat) for chloramphenicol acetyltransferase from streptomyces acrimycini and a gene (hyg) for hygromycin phosphotransferase from streptomyces hygroscopicus were subcloned in different positions of the brevibacterium lactofermentum plasmid pul340. both resistance genes are expressed in b. lactofermentum from their own pro ... | 1987 | 3479377 |
| enzymatic bases for the fatty acid positioning in phospholipids of brevibacterium ammoniagenes. | positional distribution of fatty acids in phospholipids from brevibacterium ammoniagenes was analyzed to find that phosphatidylethanolamine consisted mainly of 1-saturated acyl 2-unsaturated acyl species while phosphatidylglycerol consisted mainly of 1-unsaturated acyl 2-saturated acyl species. three acyltransferase systems were characterized in a membrane preparation--the acylations of glycerophosphate, 1-acyl-glycerophosphate, and 2-acyl-glycerophosphate--which appeared to be catalyzed by diff ... | 1986 | 3511845 |
| occurrence, biochemistry and physiology of phenazine pigment production. | | 1986 | 3532716 |
| a shuttle vector system for brevibacterium lactofermentum. | we have constructed a shuttle vector that replicates in escherichia coli, corynebacterium glutamicum and brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from b. lactofermentum and a derivative of pbr322. resistance to erythromycin which is expressed in all three bacteria has been a useful selective marker. the frequency of homospecific transformation was 1.5 x 10(5) transformants/micrograms of hybrid plasmid dna. | 1986 | 3549456 |
| manganese transport in brevibacterium ammoniagenes atcc 6872. | uptake of manganese by brevibacterium ammoniagenes atcc 6872 was energy dependent and obeyed saturation kinetics (km = 0.65 microm; vmax = 0.12 mumol/min per g [dry weight]). uptake showed optima at 27 degrees c and ph 9.5. 54mn2+ accumulated by the cells was released by treatment with toluene or by exchange for unlabeled manganese ions, via an energy-dependent process. co2+, fe2+, cd2+, and zn2+ inhibited manganese uptake. inhibition by cd2+ and zn2+ was competitive (ki = 0.15 microm cd2+ and 1 ... | 1987 | 3597325 |
| immobilization of enzymes and microorganisms by radiation polymerization. | | 1987 | 3600297 |
| structure and function of the trp operon control regions of brevibacterium lactofermentum, a glutamic-acid-producing bacterium. | the trp genes of brevibacterium lactofermentum lie within a 7.72-kb hapii-bamhi fragment whose sequence has been determined (matsui et al., 1986). the 5'- and the 3'-flanking regions of this gene cluster were subcloned as a 1.8-kb psti-psti and a 0.6-kb xhoi-bamhi fragment, respectively. the 5'-flanking region encodes two open reading frames (orfs); one corresponds to trpl, while the other corresponds to the n-terminal half of the trpe gene. within the 17 amino acid residues of the predicted lea ... | 1987 | 3609747 |
| two single-base-pair substitutions causing desensitization to tryptophan feedback inhibition of anthranilate synthase and enhanced expression of tryptophan genes of brevibacterium lactofermentum. | a 5-fluorotryptophan-resistant mutant, termed 1041, was isolated from brevibacterium lactofermentum aj12036. the anthranilate synthase of 1041 was insensitive to feedback inhibition by tryptophan, and the specific activities of the anthranilate synthase and anthranilate phosphoribosyltransferase of 1041 were 29- and 23-fold higher than those in parental strain aj12036, respectively. a single-base change (adenine to cytosine) that resulted in a ser-to-arg substitution was found in the trpe struct ... | 1987 | 3667535 |
| separation of flavins and flavin analogs by high-performance liquid chromatography. | | 1986 | 3702688 |
| induction of mutants resistant to antibiotics in brevibacterium flavum. | the optimum conditions for the induction of mutants resistant to antibiotics in brevibacterium flavum atcc 14067 were determined. uv irradiation at the energy fluence of 6.5 kj/m2 and n-methyl-n'-nitro-n-nitrosoguanidine (1 mg/ml) at ph 6.0 were used for the induction of mutants. mutant strains resistant to rifampicin, oleandomycin, streptomycin and erythromycin were prepared. | 1986 | 3710319 |
| regulation of nitrile-hydratase synthesis in a brevibacterium species. | the regulation of nitrile-hydratase biosynthese was studied in brevibacterium sp r 312. enzyme biosynthesis was not influenced by any carbon and nitrogen source used in the growth medium. it was, however, repressed by amide and amide analogues. acetamide repressed nitrile-hydratase biosynthesis and induced the wide-spectrum amidase. therefore, it appeared reasonable to hypothesize a single repressor gene for the nitrile-hydratase/wide-spectrum amidase system. | 1986 | 3729377 |
| temperature- and growth-phase-dependent changes in membrane fatty acid compositions of brevibacterium ammoniagenes. | fatty acids newly synthesized by brevibacterium ammoniagenes grown at different temperatures were analyzed. the assay temperature, not the growth temperature, was found to be the major factor affecting the unsaturated/saturated ratio of newly synthesized fatty acids in logarithmic-phase cells. however, in the stationary-phase cells the growth temperature also affected the product profile significantly; cells grown at 7 degrees c produced relatively more oleate and stearate and less palmitate and ... | 1986 | 3729431 |
| nitrile hydratase of brevibacterium r312--purification and characterization. | nitrile hydratase was purified and crystallized from the crude extract of brevibacterium r312 and found to be homogeneous by the results of disc gel electrophoresis, analytical ultracentrifuge and double diffusion in agarose. the enzyme has a molecular mass of about 85,000 da and contains approximately 3 g atoms iron/mol enzyme. the enzyme was composed of two kinds of subunits, of which molecular masses were 26,000 da and 27,500 da. the concentrated solution of the enzyme had a pronounced greyis ... | 1986 | 3768004 |
| genetic transfers in brevibacterium sp. | a positive genetic transfer by protoplast fusion was obtained in auxotrophic mutants brevibacterium sp. m27 his and brevibacterium sp. m27 arg. transformation and protoplast fusion with liposomes (as genetic transfers in intact cells and their protoplasts by both the chromosomal and plasmid dna) did not lead to transfer of the markers followed. | 1986 | 3770592 |
| an efficient method for the introduction of viral dna into brevibacterium lactofermentum protoplasts. | a method for the introduction of a bacteriophage dna into brevibacterium lactofermentum protoplasts is described. frequencies of 10(5) infective centres per micrograms dna were easily achieved, the relationship between the number of infective centres and the amount of dna being linear up to 5 micrograms dna per assay. this method can be used to introduce foreign dna into these bacteria. | 1986 | 3806056 |
| complete nucleotide and deduced amino acid sequences of the brevibacterium lactofermentum tryptophan operon. | | 1986 | 3808947 |
| microbial phosphorylation of the oa-6129 group of carbapenem compounds. | the oa-6129 group of carbapenem antibiotics were phosphorylated with atp by brevibacterium ammoniagenes at the primary hydroxyl group of the c-3 pantetheinyl side chain. the phosphorylation resulted in the reduced antimicrobial activity against some gram-positive bacteria, and the improved activity against some gram-negative microbes. the increased resistance of the oa-6129 carbapenems due to phosphorylation was significant to mouse renal dehydropeptidase and moderate to the human enzyme. oa-612 ... | 1985 | 3839225 |
| characteristics of viable brevibacterium linens cells, methionine and cysteine in milkfat-coated microcapsules. | the potential for microencapsulation of viable brevibacterium linens with methionine or cysteine in milkfat to produce sulphur compounds was examined in this study. more than 80 per cent of b. linens cells were encapsulated and then numbers inside capsules increased about three-fold during 48 h at 26 degrees c under anaerobic conditions before a slow decline. most of micro-organisms (7 x 10(7)/ml) were still viable in the capsules after 15 days. more than 90 per cent of cysteine and methionine w ... | 1985 | 3880486 |
| [hydrogenase activity in brevibacterium flavum]. | the activity of hydrogenase was assayed in the intact cells and subcellular fractions of brevibacterium flavum. the organism was shown to have the membrane-bound form of hydrogenase. the soluble nad+-reducing hydrogenase was not found. oxygen inhibited the hydrogenase activity, and its action was reversible. molecular hydrogen activated the hydrogenase of b. flavum, which was shown to be a constitutive enzyme. | 1985 | 3903443 |
| inhibition of candida albicans by methanethiol produced by brevibacterium linens. | brevibacterium linens was screened for antifungal activity against candida albicans using several antibiotic assay methods. the growth of c. albicans was inhibited only when the dual culture assay method was employed and using methionine-supplemented media. results suggest that methanethiol which is produced by b. linens' utilization of methionine is the agent inhibitory to c. albicans' growth. | 1985 | 3906369 |
| cloning and expression of tryptophan genes from brevibacterium lactofermentum in escherichia coli. | a gene bank from the amino acid producer brevibacterium lactofermentum has been prepared in escherichia coli using pbr322 as vector. four clones containing genetic information needed to complement mutations in a,b,c and d genes from e. coli have been isolated. the cloned fragments range between 4.3 kb (pult61) and 7.9 kb (pult62). all the four clones contain genetic information that complements trpb gene from e. coli. the cloned trpb gene is very stable and is maintained extrachromosomally in e. ... | 1985 | 3910042 |
| advances in the biotechnology of lysine production. | | 1985 | 3921882 |
| high-frequency transformation of brevibacterium lactofermentum protoplasts by plasmid dna. | an efficient polyethylene glycol-assisted method for transformation of brevibacterium lactofermentum protoplasts that uses plasmid vectors has been developed. two small plasmids, pul330 (5.2 kilobases) and pul340 (5.8 kilobases), both containing the kanamycin resistance gene from transposon tn5 and the replication origin of the natural plasmid pbl1 of b. lactofermentum, were selected as vectors. supercoiled forms of the plasmids yielded a 100-fold higher transformation frequency than did linear ... | 1985 | 3980445 |
| production of methanethiol from methionine by brevibacterium linens cnrz 918. | the conditions under which brevibacterium linens cnrz 918, a strain isolated from the surface smear flora of gruyère de comté cheese, produced methanethiol from methionine were studied. demethiolation was estimated from the methanethiol production capacity of resting cells. methionine was demethiolated mainly during the exponential growth phase of the organism during which time the cells were rod-shaped and had a generation time of 5 h, and the medium became alkaline. at the end of growth (ph 9) ... | 1985 | 3989511 |
| survival of brevibacterium linens during nutrient starvation and intracellular changes. | the present work reports the survival capacity of a strain of brevibacterium linens isolated from a french camembert cheese and the ensuing changes in cell composition. exponentially growing cells were harvested, washed and resuspended with shaking in ph 8.0 buffer at 21 degrees c in the absence of a carbon source. the viability of this strain, assessed with slide cultures, is much less than that of coryneform bacteria isolated from soil samples, even though no cell lysis was detected. intracell ... | 1985 | 3994487 |
| structural studies of a mannitol teichoic acid from the cell wall of bacterium n.c.t.c. 9742. | degradative and n.m.r.-spectroscopic studies have been carried out on a novel mannitol teichoic acid extracted from the cell wall of bacterium n.c.t.c. 9742, for which the name brevibacterium iodinum has been proposed. the backbone of the polymer is a poly(d-mannitol phosphate) containing 1----6 phosphodiester linkages. in most residues, pyruvic acid is acetal-linked to positions 4 and 5 of the mannitol. about half of the mannitol residues carry a beta-d-glucopyranosyl substituent at position 2. ... | 1985 | 3994675 |
| purification and some properties of phosphoenolpyruvate carboxylase from brevibacterium flavum and its aspartate-overproducing mutant. | phosphoenolpyruvate (pep) carboxylases (pc) were purified from a wild strain and an aspartate-producing mutant of brevibacterium flavum to electrophoretic homogeneity. the molecular weights of the enzymes were determined to be 4.1 x 10(5) by the gel-filtration technique. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme gave only one protein band with a molecular weight of 1.07 x 10(5). the enzyme was labile and stabilized by substrate pep, activators, metallic cofactors, a ... | 1985 | 4030719 |
| biosynthesis of trehalose by brevibacterium flavum: use of long range 13c-13c coupling data to characterize triose phosphate isomerase activity. | the 13c isotopic labeling pattern in the disaccharide trehalose (1,1'-alpha-alpha-d-glucose) produced by the microorganism brevibacterium flavum when grown on a medium containing [1-13c]glucose has been determined. long range coupling between c-1 and c-6 carbons of the glucose units can be observed in the excreted material. it is proposed that some of the 13c isotopomers in the excreted trehalose reflect the labeling pattern in (unobserved) fructose 1,6-diphosphate. analysis of the label distrib ... | 1985 | 4041565 |
| mapping of acyl carrier domain within the subunit of type i bacterial fatty acid synthetase. | a fluorescent thiol reagent, n-(7-dimethylamino-4-methylcoumarinyl) maleimide, was used to label the acyl carrier site of the bacterial fatty acid synthetase from brevibacterium ammoniagenes. the reagent bound preferentially to the 4'-phosphopantetheine thiol group of the acyl carrier domain and irreversively inactivated the enzyme. the modified enzyme was cleaved by proteinases for the mapping of the labeled site. the fluorescent fragment was readily detected on a polyacrylamide gel after elect ... | 1985 | 4074750 |
| construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria brevibacterium lactofermentum and corynebacterium glutamicum. | novel cloning vectors for glutamic acid-producing bacteria have been constructed. two cryptic plasmids, pam330 from brevibacterium lactofermentum and phm1519 from corynebacterium glutamicum, were used as precursors, and recombined with pbr325 or pub110. resultant composite plasmids were able to propagate and to express the cmr or kmr phenotype in b. lactofermentum and c. glutamicum. a smaller, high-copy-number plasmid, paj43, was also isolated following deletion of a part of the pam330-pbr325 co ... | 1985 | 4092934 |
| synergistic inhibition of phosphoenolpyruvate carboxylase by aspartate and 2-oxoglutarate in brevibacterium flavum. | purification procedures for phosphoenolpyruvate carboxylase from b. flavum were improved by using hydrophobic chromatography. the carboxylase showed optimum ph values of 7.2 and 8.0 with mn2+ and mg2+ as metallic activators, respectively. purified phosphoenolpyruvate carboxylase was found to be synergistically inhibited by aspartate and 2-oxoglutarate in the absence or presence of an activator, acetyl-coa. similarly to the aspartate inhibition, 2-oxoglutarate alone inhibited the enzyme competiti ... | 1985 | 4093449 |
| the reversibility of the adenylate cyclase reaction. | | 1971 | 4106365 |
| ultrastructure of two species of oil-degrading marine bacteria. | | 1973 | 4119518 |
| [evaluation of methods for determining dehydrogenases and means of disintegrating the cells of brevibacterium spl 22l in studying their activity]. | | 1972 | 4148968 |
| [taxonomic studies of gram-positive organisms in sewage]. | | 1974 | 4154648 |
| [study of bacteria from the dry soils of morocco: ultrastructure of brevibacterium halotolerans]. | | 1969 | 4180583 |
| effect of aflatoxins on microorganisms. | | 1972 | 4197365 |
| na+-dependent transport of threonine in brevibacterium flavum. | | 1973 | 4198929 |
| psychotrophic gram-positive bacteria: temperature effects on growth and solute uptake. | | 1973 | 4201696 |
| the conversion of bisnorbiotin and bisnordethiobiotin to biotin and dethiobiotin, respectively, by microorganisms. | | 1973 | 4204631 |
| aetiological factors in ovine posthitis. | | 1973 | 4204713 |
| studies on dextranase. ii. new exo-dextranase from brevibacterium fuscum var. dextranlyticum. | | 1974 | 4210084 |
| effect of diethylstilbestrol on feedlot associated bacteria. | | 1974 | 4215508 |
| [morphological characteristics verus the physiological and biochemical regularities of the activity of microoroganisms during periodic and continuous cultivation (literature review)]. | | 1974 | 4218032 |
| [microbiological transformation of herbicides in co-oxidation conditions]. | | 1974 | 4218859 |
| reversible inactivation of phosphofructokinase from brevibacterium liquefaciens. | | 1971 | 4259268 |
| adenyl cyclase of brevibacterium liquefaciens. | | 1967 | 4295782 |
| studies on oxygenases. i. comparative studies on 3,4-dihydroxyphenylacetate-2,3-oxygenase and pyrocatechase by electron spin resonance spectroscopy. | | 1969 | 4309720 |
| effect of dipicolinic acid on bacterial cyclic 3,5'-nucleotide phosphodiesterase. | | 1970 | 4319607 |
| [the adsorption of soil bacteria on substrates with special reference to secondary clay minerals and ionic exchangers on the basis of artifical resin. 1]. | | 1970 | 4323362 |
| the extent of reversibility of the reaction. | | 1971 | 4327490 |
| on the equilibrium of the adenylate cyclase reaction. | | 1971 | 4328843 |
| cis-terpin hydrate metabolism by a brevibacterium: patterns of enzyme induction, and accumulation of -terpineol in growth. | a brevibacterium, strain th-4, previously isolated by aerobic enrichment on the monocyclic monoterpenoid cis-terpin hydrate as a sole carbon and energy source, was found to grow on alpha-terpineol and on a number of common sugars and organic acids. oxidation of these terpenoids was shown to occur via an induced enzyme system, as measured manometrically by oxygen uptake and prevention of protein synthesis with chloramphenicol or puromycin. oxidation of terpin hydrate by cell suspensions appeared ... | 1972 | 4336108 |
| oxidative phosphorylation in bacteria which contain different cytochrome oxidases. | | 1973 | 4354617 |
| quinones of brevibacterium. | | 1974 | 4365098 |
| [bacterial enzymology: adenylate cyclase]. | | 1974 | 4365638 |
| adenylate cyclase from brevibacterium liquefaciens. iii. in situ regulation of adenylate cyclase by pyruvate. | in the presence of dl-alanine intracellular cyclic amp in nonproliferating cells of brevibacterium liquefaciens increased rapidly to the maximum level of approximately 180 mum, and extracellular cyclic amp increased to 100 mum within 4 hr at 25 degrees . adenylate cyclase (ec 4.6.1.1) induction was not observed during this incubation. the concentration of pyruvate in the total culture increased concomitantly with that of cyclic amp and reached approximately 20 mm after 4 hr of incubation. since ... | 1974 | 4373721 |
| [enzymes of microorganisms-with special reference to adenylate cyclase]. | | 1974 | 4374562 |
| regulation of aspartate family amino acid biosynthesis in brevibacterium flavum. i. inhibition by amino acids of the enzymes in threonine biosynthesis. | | 1968 | 4386082 |
| regulation of aspartate family amino acid biosynthesis in brevibacterium flavum. 3. properties of homoserine dehydrogenase. | | 1970 | 4394351 |
| regulation of nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase from brevibacterium flavum, a glutamate-producing bacterium. | | 1970 | 4394939 |
| bacterial genome sizes determined by dna renaturation studies. | | 1970 | 4396963 |
| regulation of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthetase in brevibacterium flavum. | | 1974 | 4415525 |
| regulation of prephenate dehydratase in brevibacterium flavum. | | 1974 | 4452665 |
| adenylate cyclase from brevibacterium liquefaciens. | | 1974 | 4453188 |
| [a spectrophotometric method for determining lysine in the culture fluid of brevibacterium 22]. | | 1974 | 4463365 |