| effects of alpha-adrenergic stimulation and blockade of plasma parathyroid hormone concentrations in cows. | experiments were designed to investigate responses of immunoreactive parathyroid hormone (pth) during alpha-adrenergic stimulation and blockade in cows. alpha-adrenergic agonists (methoxamine, phenylephrine and noradrenaline, the beta-adrenergic action of which was blocked by propranolol) did not change pth and free fatty acid levels, whereas they characteristically increased the blood pressure and decreased the heart rate. in contrast, alpha-adrenergic blockade by phentolamine progressively inc ... | 1978 | 27932 |
| allogeneic hematopoietic grafts after total-body irradiation in dogs with spontaneous tumors. | | 1978 | 28420 |
| a spectral probe near the subunit catalytic site of glutamine synthetase from escherichia coli. reduced pyridoxal 5'-phosphate.enzyme complexes. | in order to label phosphate binding sites, unadenylylated glutamine synthetase from escherichia coli has been pyridoxylated by reacting the enzyme with pyridoxal 5'-phosphate followed by reduction of the schiff base with nabh4. a complete loss in mg2+-supported activity is associated with the incorporation of 3 eq of pyridoxal-p/subunit of the dodecamer. at this extent of modification, however, the pyridoxylated enzyme exhibits substantial mn2+-supported activity (with increased km values for at ... | 1978 | 29046 |
| microcalorimetric study of the effects of cephalexin and cephaloridin on escherichia coli and staphylococcus aureus. | the kinetics of the antibacterial actions of cephalexin and cephaloridin against a strain of escherichia coli and a strain of staphylococcus aureus were studied by a flow microcalorimeter. the heat production was related to the number of viable organisms (cfu ml-1), the ph, the optical density of the culture medium (od540), and the morphology of the antibiotic-exposed bacteria. no heat effects could be registered when the number of cfu was below 10(4) ml-1. the addition of cephalexin, 2.5 microg ... | 1978 | 29439 |
| evaluation of the counterimmunoelectrophoretic (cie) procedure in a clinical laboratory setting. | the counterimmunoelectrophoretic (cie) procedure was evaluated under clinical laboratory conditions to determine its validity and comparability with culture methods. the procedure was further evaluated to determine applicability to a variety of clinical samples. an inexpensive set-up was developed to utilize the cie procedure at bench level. results indicated the procedure to be sensitive in detecting haemophilus influenzae, type b, and neisseria meningitidis (meningococcus), group b. the proced ... | 1978 | 29483 |
| inactivation of gamma-glutamyl transpeptidase by phenylmethanesulfonyl fluoride, a specific inactivator of serine enzymes. | | 1978 | 29617 |
| evaluation of a kinetic method for simultaneous determination of conjugated and unconjugated bilirubin. | this paper describes a fast kinetic method for the simultaneous determination of unconjugated and conjugated bilirugin in the same reaction solution. a stopped-flow mixing system with a stabilized photometer and small computer is used to mix sample and reagent rapidly and to record 250 data points during a 700-ms reaction time, and a regression program is used to resolve these kinetic data into the concentrations of unconjugated and conjugated bilirubin. data are reported for synthetic single an ... | 1978 | 29721 |
| kinetics of antimicrobial action. | | 1978 | 30169 |
| the ph-dependence of the non-specific esterase activity of carboxypeptidase a. | the hydrolysis of the following 6 esters by bovine pancreatic carboxypeptidase a (peptidyl-l-amino-acid hydrolase, ec 3.4.12.2) has been investigated over the range ph 5--10 at 25 degrees c, ionic strength 0.2: ch3co2chrco2h (r = c6h5 (ester 3), c6h5ch2 (ester 4), 4-no2c6h4co2chrco2h (r = c6h5 (ester 5), c6h5ch2 (ester 6), ch3(ch2)2 (ester 7)), ch3ch2co2ch(ch2c6h5)-co2h (ester 9). for each ester the ph dependence of kcat/km indicates that substrate binding is controlled by an acid of pkeh = 9.2 ... | 1978 | 31187 |
| isolation of anaerobes in ludwig angina. | | 1979 | 31420 |
| [application of the main components method to studying the character of the distribution of humoral antibacterial antibodies in patients with chronic bronchitis and bronchial asthma]. | | 1978 | 31749 |
| [studies on staphylococcal enterotoxin b. ii. production and regulation (author's transl)]. | enterotoxigenic strain of staphylococcus aureus (atcc 14458) was grown under various conditions with constant shaking to determine the requirements for maximum toxin production. it was evident that 3% tryptic soy broth, 3% nz-amine nak + 3% casein hydrolysate, 3% nz-amine nak + 1% yeast extract, and 3% nz-amine nak + 1% yeast extract + 0.2% glucose are most available toxin production media. but concentration of glucose could strictly triggered the enterotoxin producing efficiency. when glucose c ... | 1978 | 32015 |
| acute suppurative parotitis caused by anaerobic bacteria: report of two cases. | staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. it may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. we describe two cases of acute suppurative p ... | 1978 | 32513 |
| injury to staphylococcus aureus during sausage fermentation. | staphylococcus aureus 196e added to a beef sausage containing starter culture and 0.5 to 2.0% glucose and incubated at 35 degrees c was unable to grow when plated on tryptic soy agar (tsa) containing 7.5% nacl. the injury, presumed to be due to the lactic acid produced during fermentation, was more pronounced at the lower concentrations of glucose (and lower acid levels). in the absence of glucose and/or starter culture, no injury was observed. when sausages containing s. aureus injured by ferme ... | 1978 | 32835 |
| effect of leukocyte hydrolases on bacteria. x. the role played by leukocyte factors, cationic polyelectrolytes, and by membrane-damaging agents in the lysis of staphylococcus aureus: relation to chronic inflammatory processes. | a heat-stable factor present in extracts of human blood leukocytes is capable of lysing young staphylococcus aureus at ph 5.0. lysis is characterized by breakdown of cell-wall components as judged by electron microscopic and biochemical analysis. the leukocyte extracts can be replaced by a variety of agents known to injure cell membranes, e.g., leukocyte cationic protein histone, polymyxin b, colimycin, phospholipase a, and lysolecithin. the mechanisms by which all these agents bring about the d ... | 1977 | 33120 |
| fluorimetric assay of cefoxitin. | | 1979 | 33143 |
| genetic analysis of staphylococcus aureus rna polymerase mutants. | spontaneous mutants of staphylococcus aureus resistant to rifampin, rifamycin sv, streptovaricin, or streptolydigin were isolated and shown to be resistant due to chromosomal rather than plasmid mutations. based on data concerning spontaneous mutation rates, genetic cotransduction rates, and in vitro sensitivity studies, four major antibiotic cross-resistance patterns were found. the genetic markers responsible for these cross-resistance patterns were shown to be separable by transduction. nonpu ... | 1979 | 33150 |
| a comparison by 220-mhz nmr of histidine hydronium ion titrations in porcine pancreatic ribonuclease and an extensively deglycosylated derivative. | 220-mhz nmr was used to observe the titration behavior of the 5 histidine residues in porcine pancreatic ribonuclease (ribonucleate pyrimidine-nucleotido-2'-transferase (cyclizing), ec 3.1.4.22) and a derivative prepared by removal of 80% of the attached carbohydrate from this glycoprotein. resonances due to histidine c-2 protons were observed over the full ph range for 3 of the residues; such resonances for the remaining 2 histidine residues broadened out as the ph was increased. resonances due ... | 1979 | 33180 |
| in vivo activity of amoxicillin and ampicillin against gram-positive bacteria: results of prophylactic studies. | in prophylactic treatment of intraperitoneal infections with streptococcus pneumoniae (types 1 and 2) and streptococcus pyogenes in mice, amoxicillin had a definite advantage over ampicillin in terms of protective effect. when the infecting agent was given to mice so as to produce an infection in the brain or lung (for example, s. pneumoniae given intracranially or intranasally), amoxicillin was also more effective prophylactically than ampicillin. | 1978 | 33219 |
| physiological pharmacokinetics of beta-lactam antibiotics: penicillin v distribution and elimination after intravenous administration in rats. | | 1979 | 33246 |
| dopamine and chronic antipsychotic drug treatment. | | 1979 | 33409 |
| [established cell lines expressing differentiated characters of liver cells (author's transl)]. | | 1978 | 33421 |
| effect of growth conditions on the antigenic components of streptococcus mutans and lactobacilli. | 1) the release of lta from chemostat-grown cultures of s. mutans and l. fermentum is dependent on the generation time and ph. 2) the growth rate affects the immunogenicity of lta and the wall polysaccharide of l. fermentum. 3) the growth of l. plantarum under glucose limitation influences the glucose content of the wall teichoic acid. the composition of the wall polysaccharide of s. mutans ingbritt may also be changed under these conditions. 4) the conditions of autoclaving used to release cell ... | 1978 | 33543 |
| bacterial allergy in allergic rhinitis and bronchial asthma. | nineteen patients suffering from allergic rhinitis and bronchial asthma were studied for bacterial allergy with staphylococcus aureus, kelbsiella pneumoniae and diplococcus pneumoniae. allergy skin tests, provocative tests and the migratory inhibition factor were employed. the correlation indicates to the authors that bacterial allergy is more important than bacterial "infection" as a cause of allergic rhinitis and asthma in many instances. this is often overlooked by practicing allergists. | 1979 | 33586 |
| laboratory studies with bl-s 578 (cefadroxil) a new broad-spectrum orally active cephalosporin. | bl-s 578 (cefadroxil) is a new orally active semisynthetic cephalosporin antibiotic with broad-spectrum antibacterial activity. the new compound was evaluated in vitro in comparison with cephalexin. some properties studies such as, antibacterial activity, binding with serum proteins and stability in acid and neutral solution at 37 degrees c for both cephalosporins were similar. in experimental infections of mice, the protective action of bl-s 578 was more effective than cephalexin against staphy ... | 1979 | 33784 |
| ph dependency of kinetic parameters and reaction mechanism of beef liver catalase. | the ph-dependence of the kinetic parameters in h2o2 decomposition by beef liver catalase was investigated. at ph 7.0, the ternary complex (ess) decomposition rate was about 100 times faster than ess formation (42 microm h2o2), and the value of the michaelis constant was 0.025 m. from ethanol competition experiments, two different proton dissociation constants of the enzyme (pke1 = 5.0, pkes2 = 5.9) were obtained for the binding of first and second h2o2 molecules. another pka value (pkes1) of 4.2 ... | 1979 | 33977 |
| glycoproteins and blood group activity. oligosaccharides of a+ hog submaxillary glycoproteins. | | 1979 | 33982 |
| influence of cultivation conditions on the production of staphylocoagulase by staphylococcus aureus 104. | high yields of staphylocoagulase from staphylococcus aureus 104 were obtained in a simple salts medium supplement with glycerol, casein hydrolysate and three vitamins. conditions of oxygen-limitation (dissolved oxygen concentration less than 2%), a ph of 7.4, a temperature of 35 degrees c and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase. | 1978 | 34009 |
| on the correlation between alpha-adrenoceptor blockade and inhibition of neuronal uptake of 3h-noradrenaline. | | 1979 | 34177 |
| limited proteolysis of glutamine synthetase is inhibited by glutamate and by feedback inhibitors. | limited proteolysis of glutamine synthetase from escherichia coli has been studied under nondenaturing conditions (ph 7.6, 20 degrees c). trypsin cleaves the polypeptide chain of glutamine synthetase into two principal fragments, mr = about 32,000 and 18,000. the covalently bound amp group is attached to the larger fragment and its presence does not affect cleavage. although the cleaved polypeptide chain does not dissociate under nondenaturing conditions, catalytic activity is lost. chymotrypsin ... | 1979 | 34614 |
| chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria. | lactamase production. reacteroides melaniongenicus, 14 | 1979 | 34624 |
| the nasopharyngeal culture in acute otitis media. a reappraisal of its usefulness. | simultaneous cultures of the nasopharynx and middle ear exudate (obtained by tympanocentesis) were obtained from 225 children (mean age, 34 months; median age, 41 months) with suppurative otitis media. a 72% prediction rate for middle ear pathogens was obtained by examining the nasopharyngeal cultures after the strict observance of two essential prerequisites: (1) the nasopharyngeal culture was immediately plated on appropriate solid agar and (2) a semiquantitative method for bacterial enumerati ... | 1979 | 34739 |
| salicylate blockade of granulocyte adherence and the inflammatory response to experimental peritonitis. | aspirin profoundly inhibited the in vitro augmentation of human and mouse granulocyte adherence to nylon fiber induced by the bacterial products escherichia coli endotoxin and staphylococcus aureus culture filtrate. granulocytes obtained from normal volunteers during the 48 hr following ingestion of aspirin did not respond normally to endotoxin stimulation. furthermore, pretreatment of mice with sodium salicylate prior to intraperitoneal infection with streptococcus pneumoniae impaired granulocy ... | 1979 | 35259 |
| [the bacterial flora of preputial space]. | the bacterial flora of the preputial space of 210 healthy males (43 children aged between 2 and 11 years, 137 males between 12 and 60 years and 30 men over 60 years) was determined by smears and cultueres from glans penis, sulcus coronarius and the adjacent prepuce. the results were grouped according to various criteria, e.g. glans covered or uncovered and age of males. differences in the distribution of germs could be found in relationship to age. in the case of an uncovered glans penis the pre ... | 1979 | 35486 |
| effect of storage at 1 degree and 4 degrees c on viability and injury of staphylococcus aureus, escherichia coli and streptococcus faecalis. | | 1979 | 35509 |
| etiology and antimicrobial therapy of acute maxillary sinusitis. | eighty-one adults with symptoms of acute sinusitis were studied by direct needle puncture and aspiration of the maxillary sinus (105 sinuses). fifty-nine bacterial strains were isolated in titers of greater than or equal to 10(4) colony-forming units/ml; streptococcus pneumoniae and haemophilus influenzae accounted for 64% of the isolates. other bacteria recovered included anaerobes (12%), neisseria species (8.5%). streptococcus pyogenes (3%), alpha-hemolytic streptococcus (3%), non-group a beta ... | 1979 | 35571 |
| factors causing the clumping reaction of streptococcal strains with human plasma. | fresh isolates of 204 strains of streptococcus haemolyticus, 75 strains of viridans group streptococcus, and 45 strains of streptococcus pneumoniae were studied for their clumping reactions with human plasma. the plasma and serum factors that clumped the streptococcal strains were compared with those that clumped a staphylococcus aureus strain. one hundred eleven strains of s. haemolyticus, 10 strains of viridans group streptococcus, and none of the strains of s. pneumoniae tested were clumped b ... | 1979 | 35572 |
| studies on the relationship between the degradative rates of proteins in vivo and their isoelectric points. | acidic proteins tend to be degraded more rapidly than neutral or basic proteins in rat liver, skeletal muscle, kidney and brain and in mouse liver and skeletal muscle. we now report a similar relationship among soluble proteins from rat lung, heart and testes, and from human fibroblasts and mouse-embryo cells grown in culture. these findings indicate that the correlation between protein net charge and degradative rate is a general characteristic of intracellular protein degradation in mammals. t ... | 1979 | 36075 |
| a microcalorimetric study on the action of penicillins on staphylococcus aureus and escherichia coli. | the heat generated by cultures of a strain of escherichia coli and of a beta-lactamase- and a non-beta-lactamase-producing strain of staphylococcus aureus were registered by a flow microcalorimeter of the heat conduction type. when exposing the non-beta-lactamase-producing strain to benzylpenicillin, an effect that appeared to correspond to the 'paradoxical zone phenomenon' was found, i.e. the inhibitory effect on the bacterial metabolism, as judged from the heat generated, was smaller when usin ... | 1979 | 36262 |
| use of the chief component method for classifying clinical physiological observations. | | 1979 | 36344 |
| the microbiology of serous and mucoid otitis media. | one hundred forty-four serous and mucoid effusions were cultured for aerobic bacteria, mycoplasma pneumoniae, and virus. thirty percent of all effusions yielded an unequivocally positive culture for aerobic bacteria. although serous effusions were culture positive as often as mucoid effusions, haemophilus influenzae was isolated predominantly from serous effusions and staphylococcus epidermidis predominantly from mucoid samples. only one of 73 effusions yielded a viral isolate (herpesvirus homin ... | 1979 | 36591 |
| new indications for old antibiotics: tetracycline, erythromycin, carbenicillin, and vancomycin revisited. | | 1979 | 36669 |
| otitis media with effusion. cytological and microbiological correlates. | a quantitative cytologic study of 83 middle ear effusions from children with otitis media with effusion (ome) was made with the use of cytocentrifuge-prepared smears and was correlated with bacteriological findings. the identification of the types of cell present in an effusion was confirmed by electron microscopic findings. the most common cell types were neutrophilic leukocytes, lymphocytes, and macrophages, but a few eosinophils were found in only one case. the cytologic profile of an effusio ... | 1979 | 36875 |
| purification and partial characterization of an antibiotic produced by myxococcus coralloides. | a strain of myxococcus coralloides producing an antibiotic capable of inhibiting growth of gram-positive bacteria was isolated. antibiotic production occurred during vegetative growth but not during myxospore formation. the antibiotic was extracted from the growth medium with chloroform and purified by adsorption on silicic acid and by preparative silica gel thin-layer chromatography. the purified antibiotic showed a resistance to heat, acid, alkali and proteolytic enzymes. chromatographic and e ... | 1979 | 37227 |
| hairy cell leukemia: differences in phagocytic capacity of cells in vitro. | hairy cells from eight patients with hairy cell leukemia were evaluated with both light and transmission electron microscopy for their capacity to phagocytose zymosan, latex, staphylococcus aureus, and pseudomonas aeruginosa. in two patients, there was no phagocytosis of any of these substances; cells from three patients phagocytosed only latex; two, all except pseudomonas; and one, all 4 substances. hairy cells became relatively smooth while in culture with staphylococcus, but no surface change ... | 1979 | 37638 |
| bactericidal action of an average dose of erythromycin in the bronchi. | a study was carried out to establish whether erythromycin stearate was bacteriostatic or bactericidal at the concentrations reached in the bronchial secretion. twenty-two patients suffering from an acute attack of chronic bronchitis, sustained by streptococci, diplococci, staphylococci or h. influenzae sensitive to erythromycin, were treated with 1500 mg erythromycin per day until symptoms regressed, usually within 3 to 5 days. the results showed that after treatment there was a dramatic reducti ... | 1979 | 38063 |
| ps-5, a new beta-lactam antibiotic. ii. antimicrobial activity. | ps-5, a new beta-lactam antibiotic, has relatively potent antimicrobial activity against gram-positive and gram-negative bacteria, especially the enterobacter groups, serratia marcescens, the proteus groups and klebsiella pneumoniae. the activity of ps-5 against many beta-lactamase-producing organisms is greater than that of cefoxitin or cefazolin. ps-5 has good therapeutic activity in mice infected with staphylococcus aureus smith or enterobacter cloacae 45. | 1979 | 38238 |
| biological characteristics of peptidoglycans of group a streptococcus and some other bacterial species. ii. immunological mechanisms involved in thrombocytolysis. | immunological mechanisms are involved in the thrombocytolytic activity of peptidoglycan of group a streptococcus, streptococcus pneumoniae and staphylococcus aureus. inactivation of particular components of complement (heating of blood serum to 56 degrees c,incubation with zymosan or nh4oh) inhibited the thrombocytolytic activity of group a streptococcus peptidoglycan. so did preincubation of group a streptococcus peptidoglycan with homologous antipeptidoglycan antibody. on the other hand, antib ... | 1978 | 38280 |
| detection of experimental bacteremia and fungemia by examination of buffy coat prepared by a micromethod. | rabbits received intravenous injections of bacteria or fungi, and a comparison was made of the abilities of broth cultures, plating after dilution either in saline solution or in distilled water containing triton x-100, and buffy coat examinations to detect the organisms in heart blood. the most sensitive method was broth culture. by microscopy or subculture of buffy coat cells prepared by centrifugation of blood in microhematocrit tubes, organisms were rapidly and regularly detected when their ... | 1979 | 38662 |
| bactericidal and bacteriostatic action of chloramphenicol against memingeal pathogens. | the bacteriostatic and bactericidal effects of chloramphenicol, ampicillin, tetracycline, and sulfisoxazole were compared against several potential meningeal pathogens. chloramphenicol is bactericidal at clinically achievable concentrations against haemophilus influenzae, streptococcus pneumoniae, and neisseria meningitidis. it is bacteriostatic against gram-negative bacilli of the family enterobacteriaceae and against staphylococcus aureus. chloramphenicol has proven highly efficacious in the t ... | 1979 | 38742 |
| the inhibition of staphylococcal beta-lactamase by clavulanic acid. | clavulanic acid inhibited both the extracellular and cell-extract beta-lactamases of the four staphylococcus aureus strains tested. the inhibition of s. aureus russell cell-extract enzyme appeared to be active-site-directed and proceeded in a first-order fashion consistent with the formation of a covalent intermediate. inhibited enzyme free of excess clavulanic acid was shown to regenerate enzyme activity slowly at ph 7.0, but the rate of reactivation increased at acid ph. when the enzyme was in ... | 1979 | 38773 |
| use of staphylococcus aureus for rapid radioimmunoassay of influenza a virus haemagglutinin. | in a rapid method for the radioimmunoassay (ria) of influenza a virus haemagglutinin, staphylococcus aureus (strain cowan i, czechoslovak state collection no mau 55/64) was used for separation of bound and free antigens. with rabbit and human immune sera, the binding of antigen-antibody complexes to heat-killed, formalin-fixed staphylocci was comparable to the double antibody technique. the time required for the completion of binding reaction was about 10 min compared to 18--24 hr required for d ... | 1979 | 39433 |
| a rapid sensitive assay for specific protein synthesis in cells and in cell-free translations: use of staphylococcus aureus as an adsorbent for immune complexes. | | 1979 | 39469 |
| magnesium and iron addition to casein hydrolysate medium for production of staphylococcal enterotoxins a, b, and c. | from comparisons of 4% n-z amine nak made with distilled water, naturally hard water, and synthetic salt solutions, it appeared that magnesium and, to a lesser extent, iron were limiting factors in the production of staphylococcal enterotoxins b and c but not a. maximum enterotoxin production with nak medium was achieved by the addition of 5 mg of mg2/ per liter (for a total of 9 mg of mg2+ per liter) and 0.5 mg of fe2+ per liter. higher levels of magnesium were not inhibitory. supplementing nak ... | 1979 | 39506 |
| induction and delayed activation of tyrosine hydroxylase in noradrenergic neurons of a1 and a2 groups of medulla oblongata of rat. | | 1979 | 39662 |
| oxidative deamination of thialysine by snake venom l-aminoacid oxidase. | thialysine is oxidatively deaminated by snake venom l-aminoacid oxidase at alkaline ph. the oxygen consumption curves show a characteristic diphasic course: the quick uptake of half a mole of oxygen per mole of substrate, in aggreement with a typical oxidative deamination, is followed by a slow extra oxygen consumption. the first product of the reaction is the corresponding alpha-oxo-epsilon-amino acid, which spontaneously cyclizes to the internal schiff base 5-6-dihydro-delta 3,1,4-thiazin-3-ca ... | 1978 | 39901 |
| polymicrobial bacteremia. | of 26,961 blood cultures taken during an 18-month period at the cincinnati general hospital, 1,715 (6%) were positive. ninety-four patients had blood cultures containing more than one organism. although aerobic and anaerobic streptococci were the most frequently isolated bacteria, a variety of microorganisms, including staphylococcus aureus and the klebsiella-enterobacter-serratia group, was isolated in different combinations depending on the underlying disease. neurological illness, malignant n ... | 1979 | 40048 |
| [synergism]. | | 1979 | 40123 |
| pollution of the sea and its effects. | marine pollution has been studied under the following groupings of effects; harm to living resources, hazards to human health, reduction of amenities and interference with other users of the sea. this paper is concerned mainly with the first two categories and their interrelation. apart from certain seabirds affected by oil, the major stocks of marine animals show no evidence of reduction by pollution. pollution effects are generally insignificant in relation to other factors governing reproduct ... | 1979 | 40238 |
| oxidation of l-glucose by a pseudomonad. | a new enzyme, d-threo-aldolse dehydrogenase (2s,3r-aldose dehydrogenase), found in pseudomonas caryophylli, was capable of oxidizing l-glucose l-xylose, d-arabinose, and l-fucose in the presence of nad+. the enzyme was synthesized constitutively and purified about 120-fold from d-glucose-grown cells. the km values for l-glucose, l-xylose, d-arabinose, and l-fucose were 1.5 . 10(-2), 4.5 . 10(-3), 2.8 . 10(-3), and 2.1 . 10(-3), respectively. d-glucose and other aldoses inhibited the enzyme react ... | 1979 | 40609 |
| proceedings of the association of european pediatric cardiologists. xvith annual general meeting, 9--12 may 1978, budapest, hungary. abstracts. | | 1979 | 40796 |
| bacterial adherence to pharyngeal cells in smokers, nonsmokers, and chronic bronchitics. | selective adherence to host mucosal surfaces is probably a requirement for colonization and infection by bacteria. since pharyngeal colonization may be an important determinant in the pathogenesis of pneumonia, we studied the adherence of 10 different bacteria to pharyngeal cells obtained from nonsmokers, smokers, and chronic bronchitics. various patterns of adherence among the different groups of subjects were found. young healthy smokers had increased adherence of streptococcus pneumoniae type ... | 1979 | 40879 |
| [a bacteriological investigation of acute purulent otitis media (author's transl)]. | | 1979 | 41035 |
| carazolol, an extremely potent beta-adrenergic blocker: binding to beta-receptors in brain membranes. | | 1979 | 41147 |
| anaerobic isolates in chronic recurrent suppurative otitis media. treatment with carbenicillin alone and in combination with gentamicin. | tympanocentesis was performed in 32 pediatric patients with chronic recurrent suppurative otitis media. the aspirate was cultured aerobically and anaerobically. aerobes were isolated from ten patients (31.2%); anaerobes from one patient; and both aerobes and anaerobes from 21 patients (65.6%). there were 46 aerobic isolates. the aerobes commonly recovered were pseudomonas aeruginosa (24 isolates) proteus sp. (5) and staphylococcus aureus (3). there were 32 anaerobes isolated including anaerobic ... | 1979 | 41811 |
| comparative adsorption of human enteroviruses, simian rotavirus, and selected bacteriophages to soils. | virus adsorption to soils is considered to be the most important factor in removing viruses after land treatment of wastewater. most of the studies on virus adsorption to soils have utilized poliovirus as the model system. in the present study, comparative adsorption of a number of different types and strains of human enteroviruses and bacteriophages to nine different soil types was studied. under the experimental conditions of this study, greater than 90% of all viruses adsorbed to a sandy loam ... | 1979 | 42350 |
| antimicrobial activity of amniotic fluid obtained vaginally. | twenty-nine amniotic fluid samples were aspirated from pregnant women at term (38-40 weeks' gestation) by tapping the bulging membrane vaginally, and their effects on escherichia coli, staphylococcus aureus and candida albicans were studied. inhibition rates were 72.41% for e coli, 68.96% for s aureus and 79.31% for c albicans. two samples (6.90%) affected only a single organism, 18 (62.07%) affected two organisms and eight (27.59%) affected all three. only one sample (3.45%) had no inhibitory e ... | 1979 | 42590 |
| an evaluation of 12 methods for the demonstration of penicillinase. | twelve methods for the demonstration of bacterial penicillinase production by strains of haemophilus influenzae and staphylococcus aureus are compared, and their suitability for routine clinical laboratory use is evaluated. the acidometric agar plate method is recommended. | 1979 | 42650 |
| quantitative analysis of proton-linked transport systems. glutamate transport in staphylococcus aureus. | 1. the magnitude of the protonmotive force in respiring staphylococcus aureus was measured over the range of extracellular ph from 5.6 to 7.8. 2. the membrane potential remains constant at 150 mv, inside-negative, but the ph gradient decreases from 2.1 units, inside-alkaline, at ph 5.6 to zero at ph 7.5 and above. 3. the accumulation of glutamate in the soluble cell pool is ph-independent at a value equivalent to 100 mv. 4. the results of experiments studying co-transport of protons are consiste ... | 1979 | 43145 |
| nonlinear dependence of biological activity on hydrophobic character: the bilinear model. | in homologous series of compounds biological activity is linearly dependent on hydrophobic character until a cut-off point is reached where this linear relationship changes to a nonlinear relationship: biological activity increases with increase of hydrophobic character, reaches a maximum and then decreases with further increase of hydrophobic character. drug transport in biological systems is determined by the rate constants of transfer of the drug through aqueous and organic compartments. in s ... | 1979 | 43264 |
| opsonic activity of normal human cerebrospinal fluid for selected bacterial species. | the opsonic activity of normal human cerebrospinal fluid (csf) has not been well defined. in this study, the opsonic activity of normal csf for laboratory and blood culture isolates of staphylococcus aureus, streptococcus pneumoniae, escherichia coli, hemophilus influenzae type b, and neisseria meningitidis was measured by a quantitative assay employing radiolabeled bacteria and polymorphonuclear leukocytes. all isolates of s. aureus, except the wood 46 strain, were opsonized in undiluted csf (> ... | 1979 | 43289 |
| effects of blood on blood culture medium. | the morphological and biochemical changes that occur after inoculation of sterile blood into a blood culture medium (tryptic soy broth) with sodium polyanetholesulfonate and co(2) were investigated. cellular changes, ph, pco(2), and po(2) were monitored and evaluated. erythrocytes became crenated and developed precipitated hemoglobin inclusions within 4 h. the lymphocytes appeared morphologically intact at 24 h, and by 48 h a few cells had undergone transformation. many neutrophils were vacuolat ... | 1979 | 43336 |
| on an in vitro method of simulating drug release from viscous eye drops. | | 1979 | 43382 |
| cryptolepine hydrochloride effect on staphylococcus aureus. | | 1979 | 43386 |
| [fluorescent probe study of staphylococcal cell wall charges]. | the effect of ph and mgcl2 concentration on the fluorescence of 1-anilinonaphthalene-8-sulfonate (ans), an anion probe, was studied in suspensions of staphylococcus aureus. the maximum of ans fluorescence shifted towards the short wavelength region of the spectrum and its intensity increased when the cells were incubated in the medium with low ph and high ionic strength. the results are discussed in terms of electrostatic interactions between the anions of the probe and the negative space charge ... | 1979 | 43463 |
| some characteristics of staphylococcus aureus (penicillin-sensitive and -resistant groups) isolated from healthy individuals (nsukka, nigeria). | | 1979 | 44164 |
| statistico epidemiological study of changes in the vaginal flora of contraceptive pill users in alexandria. | a stratified random sample of 1000 women with proportionate allocation according to district of residence was taken from normal females living in alexandria, egypt, and attending family planning centers in order to understand social-pathological changes in the vaginal flora of oral contraceptive (oc) users. cases were examined over 18 months, and all cases were given a combined oc. bacteriology and ph changes in vaginal flora were determined after 18 months. results of the bacteriological exa ... | 1979 | 44312 |
| evaluation of phagocytic and bactericidal activities of neutrophil granulocytes. determination of viable extracellular bacteria by their incorporation of 14c-leucine and 3h-thymidine. | a method for evaluation of human neutrophil granulocyte function based on the combined determination of total and extracellular bacteria is described. the total number of surviving bacteria is assessed by the determination of colony forming units (cfu) after hypotonic lysis of granulocytes. extracellular viable bacteria can be determined by the incorporation of 14c-leucine or 3h-thymidine into bacterial macromolecules since there is a linear relationship between macromolecular synthesis and bact ... | 1979 | 44387 |
| [hydrotherapy pools, microbiological and chemical results (author's transl)]. | the results of microbiological and chemical examinations of water samples collected from hydrotherapy pools for non incontinent patients proved that the officially agreed parameters for the surveillance of public swimming pools (osterr. bäderhygiene-gesetz and verordnung) have to be extended to meet the requirements of epidemiology. similarly the requirements for treatment and operating conditions of those waters should be more rigorous. minimal requirements for the treatment of water in hydroth ... | 1979 | 44417 |
| [bacteriological studies of the intestinal content of aquatic birds, fishes, and frogs with special reference to the presence of non-cholera vibrios (ncv) (author's transl)]. | in a screening study of surface waters, the authors were successful in culturing ncv in 2/3 of cases. since these organisms are incapable of multiplication in open waters and yet were present in water samples in considerable amounts, it was postulated that they persisted in certain forms of aquatic life. to elucidate this question, the intestinal contents and in some cases, the bile of a total of 110 animals belonging to 17 different bird, fish, and frog species from different habitata were exam ... | 1979 | 44627 |
| human monocyte killing of staphylococcus aureus: modulation by agonists of cyclic adenosine 3',5'-monophosphate and cyclic guanosine 3',5'-monophosphate. | this study was designed to test whether cyclic nucleotides play a role in the regulation of bacterial killing by human monocytes. agents were tested for their ability to activate monocyte adenylate or guanylate cyclase in cell-free preparations, to increase cyclic adenosine 3',5'-monophosphate (camp) or cyclic guanosine 3',5'-monophosphate (cgmp) in intact human monocytes, and to modulate monocyte-induced killing of staphylococcus aureus in vitro. prostaglandin e1 and cholera toxin activated mon ... | 1979 | 44704 |
| comparative study of erythromycin, amoxicillin and ampicillin antimicrobial activity against human respiratory tract pathogens. | an in vitro test system was used to compare the antimicrobial activity of erythromycin, amoxicillin and ampicillin against respiratory tract pathogens isolated from man. the minimum inhibitory concentrations (mics) of fresh clinical isolates of streptoccus pyogenes, streptocuccus pneumoniae, staphylococcus aureus and haemophilus influenzae to the macrolide and penicillins ranged between 0.01 and 0.9 microgram/ml. the microbes were exposed to each antibiotic for approximately 3 h at 1x,2x and 5x ... | 1979 | 44765 |
| [animal experiments with combined local immunization against an influenza virus disease (author's transl)]. | | 1979 | 44774 |
| a simple method of detecting staphylococcal hemolysins. | a modification of the "one-plate method" for detection of staphylococcal hemolysins has been described. bacterial strains of staphylococcus aureus, streptococcus agalactiae and cornebacterium pseudotuberculosis were replaced by their prepurified hemolytically active exosubstances: beta-toxin, camp-factor, and, the exosubstance of corynebacterium pseudotuberculosis called corex. by means of the modification described, exact detection of hemolysins in possible with staphylococcal strains producing ... | 1979 | 44782 |
| cefaclor: clinical and bacteriological evaluation in 45 cases. | this report deals with studies of the effectiveness, tolerance and toxicity of cefaclor in the treatment of 45 patients with urinary tract infections (40 cases) and pneumonia (5 cases) caused by susceptible pathogens. cefaclor was administered orally in a dosage of 1 to 3 g per day for 7 to 11 days. clinical and laboratory examinations were performed before, during, at the end of treatment, and 4 weeks later in cases of urinary infection. all 5 patients with pneumonia and bronchopneumonia were c ... | 1979 | 44909 |
| [improved method for the demonstration of protein a of staphylococcus aureus (author's transl)]. | protein a (pa) could be extracted completely from staphylococcus aureus by treatment with concentrated formic acid. this led to the development of a semi-quantitative determination of pa by hemagglutination (fig. 1). the treatment with formic acid yielded pa more effectively than the commonly used extraction by boiling (table 1). it could be conducted directly on a loopfull of staphylococci obtained from blood agar. it required no additional cultivation in a fluid medium. most suitable for the h ... | 1979 | 44938 |
| interactions of pharmacological agents which alter biogenic amine metabolism and depression--an analysis of contributing factors within a primate model of depression. | the observation that the biogenic amine depleting agent, reserpine, could induce severe depression in a small proportion of the patients treated with it has proved to be seminal finding in what is now a much larger field of research relating the function brain biogenic amine systems to emotions and behavior. a review of the human reserpine literature suggests, however, that factors other than pharmacologically produced alterations in brain biogenic amine metabolism must have been critical determ ... | 1979 | 45183 |
| emergence of antibiotic resistance in hospitals, 1935-1975. | a limited review of the changes in susceptibility of common bacterial pathogens to available antibacterial agents is presented. significant developments in recent years include the following: (1) the emergence of streptococcus pneumoniae with decreased resistance to penicillin and of some strains resistant to several antibiotics; (2) a decline in prevalence of multi-drug-resistant staphylococcus aureus after 1960 following their increasing prevalence in the preceding years (these changes were me ... | 1979 | 45521 |
| a comparison of staphylococcal nasal carrier rates in germany and poland. | frequencies of different staphylococcal nasal carrier classes were studied in groups of students of nursing and of laboratory personnel in cologne, west germany, and in krakow, poland, by six consecutive samplings of their nasal vestibules. the general number of persistent carriers of s. aureus appeared to be lower than in previous studies, paralleling the diminished numbers of staphylococcal infections in populations. differences in numbers of persistent carriers between the cologne and krakow ... | 1978 | 45603 |
| letter: in-vitro minocycline activity against tetracycline-resistant staphylococcus aureus. | | 1975 | 46562 |
| effect of microwaves on cell function and virus replication in cell cultures irradiated in vitro. | | 1975 | 46732 |
| sulfhydryl-dependent thermal aggregation of human gamma globulin: augmentation by hyaluronic acid. | hyaluronic acid (4 mg/ml) augmented elevenfold the copper-catalyzed (7 mum) thermal (63 degrees c, 2 hours) aggregation of human gamma globulin (2 mg/ml) in 0.075 m phosphate buffer, ph 7.4. almost no augmentation of aggregation occurred with hyaluronidase-treated hyaluronate. hyaluronate-augmented copper-catalyzed thermal aggregation was inhibited by l-histidine, gold thiomalate, n-ethylmaleimide, p-chloromercuribenzoic acid, and ethylenediaminetetraacetic acid. together with previous reports o ... | 1975 | 46754 |
| fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria. | the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ... | 1975 | 46774 |
| letter: in-vitro minocycline activity against tetracycline-resistant staphylococcus aureus. | | 1975 | 47049 |
| letter: minocycline activity against tetracycline-resistant staphylococcus aureus. | | 1975 | 47564 |
| immunocytological methods for the identification and localization of antigens. | immunocytologic techniques allow the study of antigenic cellular constituents by optical or electron microscopy, antibodies labelled with tracers such as fluorochromes, enzymes, radioactive isotopes, or large electron-dense molecules form the basis of these techniques. the immunochemical procedures involved in the preparation of such conjugates are well defined. satisfactory results, however, also depend upon the proper preparation and processing of the tissue or cell suspension being studied. | 1975 | 47687 |
| virulence of genus staphylococcus. iii. studies on the pathogenicity to mice. | six representative strains of staphylococci were selected on the basis of dnase activity and inoculated into the mice to study the correlation of the presence of this enzyme to the virulence of the staphylococci. two strains of staphylococci among them produced dnase but not the free coagulase, and one of these two strains had an obvious virulence against the mice. the results of experiments suggest that there are at least certain strains which may be designated as staphylococcus aureus among th ... | 1975 | 47790 |
| enhanced bactericidal activity of phagocytes from patients with chronic granulomatous disease in the presence of sulphisoxazole. | the decrease in the frequency and severity of bacterial infections in four of five children with chronic granulomatous disease (c.g.d.) on long-term sulphonamide therapy was out of proportion to demonstrable direct antibacterial effects of the drug. in an attempt to determine the mechanism for this apparent protective effect, the killing of sulphisoxazole-resistant escherichia coli and staphylococcus aureus by leucocytes from five patients with c.g.d. was studied in the presence or absence of 5- ... | 1975 | 48055 |
| emergency! part 3: on-the-spot care for aspiration, burns, and poisoning. | | 1975 | 48222 |
| phagocytosis in patients and carriers of chronic granulomatous disease. | the phagocytic capacity of leucocytes from four patients and two carriers of chronic granulomatous disease (c.g.d.) was compared to that of normal leucocytes. leucocytes from c.g.d. patients phagocytised more staphylococcus aureus than did normal leucocytes after 5, 10, and 20 minutes of incubation. phagocytosis by leucocytes from two carriers of c.g.d. was intermediate between that in the patients and in controls. in contrast, phagocytosis of streptococcus faecalis, an organism readily killed b ... | 1975 | 48723 |