| diagnosis of sickle cell disease in chronically transfused patients. | standard electrophoretic methods for the diagnosis of hemoglobinopathies are confounded in individuals chronically transfused. we present the accurate diagnosis of sickle cell disease in two such transfused patients by the application of polymerase chain reaction technology to analyze patient's hemoglobin beta-chain genes directly. | 1992 | 1550267 |
| modulation of venular microvessel permeability by calcium influx into endothelial cells. | it has been proposed that calcium ion influx into endothelial cells modulates the permeability of venular microvessels via a calcium-dependent contractile process. the results of recent investigations using permeabilized endothelial cell monolayers conform to this hypothesis by demonstrating a calcium-dependent interaction of endothelial actin and myosin during the retraction of adjacent endothelial cells exposed to inflammatory agents. little is known about the pathway for calcium influx into e ... | 1992 | 1563597 |
| [cryptococcosis in aids patients: a study of 19 cases]. | we discuss 19 cases of infection due to cryptococcus neoformans diagnosed in 438 aids patients admitted to our center (4%). fourteen of them showed meningitis confirmed by culture of c. neoformans in csf. clinical features were rather unspecific and disorders in csf parameters were non striking. the diagnostic techniques performed with best results were culture of c. neoformans and antigen determination, especially in serum. survival probability at one year was 75%. treatment response was good. ... | 1992 | 1626090 |
| the rat mast cell antigen ad1 (homologue to human cd63 or melanoma antigen me491) is expressed in other cells in culture. | previously we reported that the mab ad1 recognized a heavily glycosylated 50- to 60-kda protein (ad1 ag) sterically close to the high-affinity ige receptor on rat basophilic leukemia (rbl-2h3) cells. the n-terminal amino acid sequence of the ad1 ag was nearly identical to that of human cd63 (melanoma-associated ag me491). in this study we cloned the cdna of ad1 ag from a rat basophilic leukemia 2h3 cdna library. an open reading frame of 238 amino acids was identified that contained the n-termina ... | 1992 | 1634775 |
| [the human chorionic gonadotropin (hcg) receptor: a new class within the family of gtp protein coupled receptors. epitope mapping of receptor-bound agonistic and antagonistic forms of hcg]. | human chorionic gonadotropin (hcg) is a member of the glycoprotein hormone family. it is composed of an alpha and a beta subunit, the latter being closely homologous to that of human luteinizing hormone (hlh). hcg and hlh bind to the same receptor. the molecular mass of hcg is 38 kd, up to 30% of which is contributed by the carbohydrate moieties linked to each of the two subunits. structure-function relationship studies have indicated that both subunits interact with the receptor and that altoge ... | 1992 | 1636261 |
| antagonism of the motor effects of cgrp and of capsaicin on the guinea pig ileum by human cgrp8-37. | | 1992 | 1637118 |
| expression and processing of the rooster protamine mrna. | in situ hybridization in immature and mature testis sections shows that the rooster protamine mrna is transcribed in the post-meiotic stages of spermatogenesis. two distinct populations of rooster protamine mrna are expressed as determined by northern blot analysis. since there are two copies of the chicken protamine gene per haploid genome, the question was raised of whether the two mrna populations correspond to the two different genes or was a result of differential mrna processing. the fact ... | 1991 | 1664677 |
| the dysmyelinating mouse mutations shiverer (shi) and myelin deficient (shimld). | shiverer (shi/shi) is an autosomal recessive mouse mutation that produces a shivering phenotype in affected mice. a shivering gait can be seen from a few weeks after birth until their early death, which occurs between 50 and 100 days. the central nervous system of the mutant mouse is hypomyelinated but the peripheral nervous system appears normal. the myelin of the cns, wherever present, is not well compacted and lacks the major dense line. myelin basic protein (mbp), which is associated with th ... | 1990 | 1693848 |
| human prolactin gene expression. the use of an alternative noncoding exon in decidua and the im-9-p3 lymphoblast cell line. | the prolactin (prl) gene is normally expressed in the anterior pituitary lactotrope and the decidualized stromal cell of the human endometrium. we have recently described the ectopic expression of the human prl gene in a b-lymphoblastoid cell line, im-9-p. the im-9-p and decidual prl mrnas are approximately 150 nucleotides longer than the pituitary transcript. the protein coding sequence of the im-9-p and decidual prl cdnas is identical to the pituitary prl message, and the 3'-untranslated regio ... | 1990 | 1697858 |
| human prolactin gene expression. the use of an alternative noncoding exon in decidua and the im-9-p3 lymphoblast cell line. | the prolactin (prl) gene is normally expressed in the anterior pituitary lactotrope and the decidualized stromal cell of the human endometrium. we have recently described the ectopic expression of the human prl gene in a b-lymphoblastoid cell line, im-9-p. the im-9-p and decidual prl mrnas are approximately 150 nucleotides longer than the pituitary transcript. the protein coding sequence of the im-9-p and decidual prl cdnas is identical to the pituitary prl message, and the 3'-untranslated regio ... | 1990 | 1697858 |
| growth hormone prevents steroid-induced growth depression in health and uremia. | treatment with supraphysiological doses of corticosteroids results in protein wasting and impairment of growth, whereas exogenous growth hormone (gh) causes anabolism and improvement of growth. we wanted to know whether the growth depressing effects of methylprednisolone (mp) are more expressed in an organism which is chronically diseased and whether these effects can be counterbalanced by concomitant treatment with recombinant human growth hormone (rhgh). mp in doses from 1 to 9 mg/kg/day cause ... | 1991 | 1762304 |
| the effect of age on motor neurone death following axotomy in the mouse. | the ability of mouse motor neurones to survive axotomy during the first month of life was studied. the motor neurones that lie in the dorsolateral columns of spinal segments c7 and c8 and supply the flexor muscles of the forepaw were axotomized by cutting and removing part of the median and ulnar nerves above the elbow. the number and position of cell bodies with axons in these nerves were confirmed by retrograde labelling of the cut axons with horseradish peroxidase. the ability of these neuron ... | 1991 | 1769343 |
| non-adrenergic, non-cholinergic neural activation stabilizes smooth-muscle tone independently of eicosanoid factors in guinea-pig isolated airways. | 1. we examined the effect of variations in resting smooth-muscle tone induced eicosanoid inhibitors on the direction and magnitude of the non-adrenergic, non-cholinergic (nanc) nervous response in guinea-pig isolated airways. 2. nanc responses (in the presence of 1 microm atropine, 10 microm guanethidine) to electrical field stimulation (1200 ma; 0.5 ms; 240 s; 3 hz) were studied in guinea-pig isolated airway preparations (n = 4-7) taken from the proximal trachea, the distal trachea and the main ... | 1991 | 1797314 |
| non-adrenergic, non-cholinergic neural activation stabilizes smooth-muscle tone independently of eicosanoid factors in guinea-pig isolated airways. | 1. we examined the effect of variations in resting smooth-muscle tone induced eicosanoid inhibitors on the direction and magnitude of the non-adrenergic, non-cholinergic (nanc) nervous response in guinea-pig isolated airways. 2. nanc responses (in the presence of 1 microm atropine, 10 microm guanethidine) to electrical field stimulation (1200 ma; 0.5 ms; 240 s; 3 hz) were studied in guinea-pig isolated airway preparations (n = 4-7) taken from the proximal trachea, the distal trachea and the main ... | 1991 | 1797314 |
| [comparative study of culture media for isolating brucella ovis]. | for the isolation of brucella ovis, modified thayer-martin medium with the addition of 0.01 micrograms/ml of trimethoprim and 100 iu of nystatin (tmm) was compared with skirrow agar (sk). using viable counting technique, 11 strains were evaluated and the results were compared with those using columbia base agar with bovine blood 7% (cba). ninety-four semen samples of 33 rams from a flock with infection antecedents were cultured on the same media. growth of brucella ovis strains was similar in al ... | 1991 | 1815277 |
| biting behavior of anopheles mosquitoes in costa marques, rondonia, brazil. | mosquito collections were made in and near costa marques, rondonia, brazil, to determine anopheline anthropophilic/zoophilic behavior. collections from a non-illuminated, bovine-baited trap and indoor and outdoor human-bait collections were compared. anopheles darlingi and anopheles deaneorum were more anthropophilic than the other anophelines collected. the remainder of the anopheles species were collected much more frequently in bovine-baited traps than in human-bait collections. anopheles dar ... | 1991 | 1815282 |
| oral administration of ssg, a beta-glucan obtained from sclerotinia sclerotiorum, affects the function of peyer's patch cells. | the effect of orally administered ssg, a beta-1,3-glucan obtained from the culture filtrate of a fungus, sclerotinia sclerotiorum ifo 9395, on the function of peyer's patch (pp) cells was investigated in comparison with that on spleen cells in mice. oral administration of ssg enhanced the proliferative response of pp cells to a t-cell mitogen, concanavalin a (con a), and a b-cell mitogen, lipopolysaccharide (lps), although the response of spleen cells was not affected. peyer's patch cells taken ... | 1991 | 1828793 |
| miniature manipulator with microelectrode for chronic recording of single units from freely-moving animals. | we report a simple recording and stimulating system, a miniature manipulator with a microelectrode (mmm) that can be mounted on animal's skull. the system consists of a miniature manipulator and a microelectrode which are used in one body. several mmms can be implanted simultaneously into the brain as the part of the manipulator is small. with this device, recording of single units from several different cortices is possible from awake and unrestricted animals. | 1991 | 1851979 |
| plasminogen polymorphism in libyans: description of a new rare variant. | the genetic polymorphism of human plasminogen (plg) was investigated in libyans using wide-scale ultrathin-layer polyacrylamide isoelectric focusing with subsequent immunoblotting. the 2 common alleles, plg*a and plg*b, and 4 previously reported rare ones, plg*a3, plg*m4, plg*b1 and plg*b2, were observed. in addition, a new intermediate rare allele designated plg*mtripoli (plg*mt) was encountered. the estimated allele frequencies for the genes plg*a, plg*b, plg*a3, plg*mt, plg*m4, plg*b1 and plg ... | 1991 | 1855784 |
| relevance of n-nitrosamines to oesophageal cancer in china. | oesophageal cancer occurs at a very high frequency in certain areas of china, especially in linxian county, henan province. previous studies suggested that n-nitroso compounds play a causative role. in order to study further the exposure of linxian inhabitants, the intake of n-nitrosamines in the diet was determined. the total daily intake of volatile nitrosamines and of n-nitroso-n-methylbenzylamine (nmbza) was higher in linxian than in two other counties. nmbza can induce cancer in animal and ... | 1991 | 1855832 |
| effects of long-term inhalation of n-nitrosodimethylamine in rats. | the toxicological evaluation and histopathological findings of a long-term inhalation study in progress with n-nitrosodimethylamine (ndma) are presented. exposure was to three concentrations of ndma: 0.04, 0.2 and 1.0 ppm (corresponding to 120, 600 and 3000 micrograms/m3). a significant reduction in median survival time (nine months) was seen in animals treated with the highest concentration of ndma. tumours occurred mainly in the nasal cavity, with the highest incidences in the groups receiving ... | 1991 | 1855872 |
| prognostic value of rhesus blood groups in oral squamous cell carcinomas. | in the current study of the prognosis of all patients (n equals 70) with squamous cell carcinomas (scc) of floor of mouth in norway during the period 1963 to 1972, the authors found that patients with rhesus (rh) (d)-negative blood group had significantly poorer prognosis (mean 5-year survival, 8%) than patients with rh (d)-positive blood group (5-year survival, 30%) (p equals 0.04). this extends the authors' previous observations in another group of oral cancer patients. the authors do not know ... | 1991 | 1913459 |
| effects of experimental hyper- and hypothyroidism on natural defense activities against lewis lung carcinoma and its spontaneous pulmonary metastases in c57bl/6 mice. | the effects of expermentally induced hyper- and hypothyroidism on the growth and development of spontaneous pulmonary metastases of lewis lung carcinoma (3ll) cells were studied in a murine system. progression of 3ll tumors growing in mice was associated with significant reduction in the serum levels of t3 and t4. subcutaneous (s. c.) injections (3 times/week) of t3 resulted in a hyperthyroid state with elevated t3 and reduced t4, whereas treatment with t4 induced a hyperthyroid state with eleva ... | 1991 | 1948998 |
| [microbiological care of ventilated intensive care patients. feasibility of diagnosis and therapy of pulmonary infection]. | pulmonary infections are the most life-threatening infections in mechanically ventilated patients. methods to avoid these infections by prophylactic systemic or local administration of antibiotics may promote resistance and selection of distinct groups of pathogens. in mechanically ventilated patients we studied the impact of early diagnosis and specific therapy on the prevention of pulmonary infections. due to the very short interval between colonization and infection, daily microscopic and mic ... | 1991 | 1952044 |
| characterization of refractory period extension by transcardiac shock. | to better understand the refractory period extension (rpe) produced by transcardiac shocks and its possible role in defibrillation, we measured rpe under various experimental conditions. | 1991 | 2040057 |
| bullous pemphigoid antigen: cdna cloning, cellular expression, and evidence for polymorphism of the human gene. | a human epidermal keratinocyte lambda gt11 recombinant cdna library was screened with a 0.45-kb cdna that was generated by polymerase chain reaction (pcr) amplification of a segment of human bullous pemphigoid (bp) antigen mrna. the screen yielded five clones, the largest one, pcbpa-4, being 2.3 kb in size. the pcbpa-4 cdna hybridized in northern analyses with an approximately 9-kb mrna from cultured keratinocytes, whereas no hybridization signal was detected with rna from human skin fibroblast, ... | 1991 | 2045679 |
| [differential roentgenological diagnosis of injuries of the lower cervical vertebrae]. | on the basis of 14 experiments with simulation of the lower cervical vertebra dislocations on cadavers and human cervical vertebra blocks, combined with stage discography, the authors singled out 3 groups of +disk and ligament structure damages: damage of capsular--ligamentous apparatus of the rear support complex; damage of ligamentous--capsular apparatus and disk; damage of ligamentous-capsular apparatus, frontal longitudinal ligament and total rupture of a disk. differentiation is made accord ... | 1990 | 2074961 |
| [recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation]. | every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ... | 1990 | 2075365 |
| oxygen-transport fluid based on perfluorochemicals: effects on liver biochemistry. | the effects of (1) i.v. injection of various perfluorochemical (pfc) emulsions, (2) different fractions of the non-ionic poloxamer surfactant, pluronic f-68, or (3) i.p. injection of component pfc oils have been studied separately in male and female rats. injection of 10 ml/kg body wt of either fluosol-da 20% (f-da) or a novel perfluorodecalin emulsion containing a c-16 oil additive in male rats increased liver weight up to 7 days later; no corresponding effect occurred in response to injection ... | 1990 | 2096632 |
| susceptibility to lysis of pulmonary alveolar macrophages by human lymphokine-activated killer cells. | in this study we addressed the question of whether lymphokine-activated killer (lak) cells, besides killing neoplastic cells, may exert a certain degree of lysis on the normal counterpart; in particular we took into consideration the toxicity against pulmonary alveolar macrophages (pam). we demonstrated that human lak cells generated in vitro following incubation of peripheral blood mononuclear cells with recombinant interleukin 2 for 4 days were able to lyse normal pam in a 4-h 51cr release ass ... | 1990 | 2106387 |
| prevalence of psychiatric disorders in a mandatory screening program for infection with human immunodeficiency virus: a pilot study. | ninety-five randomly selected human immunodeficiency virus (hiv)-seropositive air force personnel were psychiatrically examined during a routine medical evaluation. of the 95, 95% did not have acquired immunodeficiency syndrome and were largely asymptomatic; 61.1% had clinical axis i diagnoses, which included simple phobia, adjustment disorders, hypoactive sexual desire disorder, alcohol use disorder, major depression, and organic mental disorders; 30.5% had personality disorders. significantly ... | 1990 | 2106656 |
| cell cycle kinetics of insect cell cultures compared to mammalian cell cultures. | cell cycle kinetic of lepidopteran cell lines sf9 (spodoptera frugiperda) and izdmb0503 (mamestra brassicae) were investigated and compared to mammalian cell cycle distributions. the resting phase (g0) of mammalian cells is characterized by a 2c-dna content whereas g0-phase of insect cell lines is characterized by a 4c-dna content. flow cytometric data in combination with growth curves of partially synchronized and asynchronously growing cells proved the existence of this phenomenon. kinetics of ... | 1990 | 2142463 |
| interactions between estrogens, androgens, progestins, and glucocorticoids in zr-75-1 human breast cancer cells. | the human breast cancer cell line zr-75-1 possesses androgen, estrogen, progesterone, and glucocorticoid receptors, thus offering a good model to study the specific role of each class of steroids in the control of breast cancer growth. although the stimulatory action of classical estrogens (e2 and estrone) is well known, we have found a potent mitogenic effect of the adrenal estrogen androst-5-ene-3 beta,17 beta-diol (delta 5-diol) at concentrations within the range of those found in the serum o ... | 1990 | 2142871 |
| preparation and characterization of anti-paroxetine antibodies. | 6-nitroparoxetine was synthesized and reduced to 6-aminoparoxetine. after coupling to glutaraldehyde at the 6-position and to bovine serum albumin, the resulting schiff's base was further reduced into an amino-derivative which served as the antigen. anti-paroxetine antibodies were raised against this antigen in rabbits and the anti-paroxetine igg purified by protein a affinity chromatography. the anti-paroxetine igg demonstrated high specificity towards paroxetine and 6-nitroparoxetine without s ... | 1990 | 2147049 |
| a short dna sequence from lambda phage inhibits protein synthesis in escherichia coli rap. | the escherichia coli rap mutant inhibits vegetative growth of bacteriophage lambda. phage mutations termed bar, which overcome the rap defect, have been mapped to three genetic loci in the pl operon. plasmids with a lambda wild-type bar dna segment cloned downstream from an active promoter cannot be maintained in rap mutant bacteria. the viability of a rap mutant strain decreases rapidly after induction of transcription through bar regions present on plasmids. under these (restrictive) condition ... | 1990 | 2147720 |
| effects of intrastriatal hormones on the dorsal immobility response in male rats. | previous research has shown that estradiol administered either peripherally or directly into the striatum potentiates the dorsal immobility response (dir) in ovariectomized female rats. male rats are even more responsive than females to intrastriatal estradiol, and furthermore respond to the effects of catecholestrogens while females do not. in order to determine whether the heightened effects of estrogens in males are due to conversion to catecholestrogens, castrated male rats were given bilate ... | 1990 | 2157227 |
| effects of intrastriatal hormones on the dorsal immobility response in male rats. | previous research has shown that estradiol administered either peripherally or directly into the striatum potentiates the dorsal immobility response (dir) in ovariectomized female rats. male rats are even more responsive than females to intrastriatal estradiol, and furthermore respond to the effects of catecholestrogens while females do not. in order to determine whether the heightened effects of estrogens in males are due to conversion to catecholestrogens, castrated male rats were given bilate ... | 1990 | 2157227 |
| genetic controls of mitochondrial development and function. genetic analysis of subunits 6 and 9 of yeast ubiquinol-cytochrome c oxidoreductase complex. | | 1990 | 2168205 |
| adrenal findings with cmv infection in aids. | | 1990 | 2168675 |
| gene-deleted ibrv marker vaccine. | | 1990 | 2175520 |
| the effects of stress during the brain growth spurt. | | 1990 | 2206630 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| radioimmunotargeting of human tumour cells in immunocompetent animals. | a tumour model system is reported that for many purposes may be an alternative to xenografted nude mice. the model allows immunotargeting of human tumour cells in immunocompetent animals. the target cells are contained in i.p. diffusion chambers (dc) with micropore membrane walls that are permeable to molecules, including the cell specific monoclonal antibodies (moab), but impermeable to cells. thus, the tumour cells are protected from the host immunocompetent cells. in the work here presented t ... | 1990 | 2223574 |
| the effect of methylxanthines on milk volume and composition, and growth of rat pups. | a study was conducted to investigate the effect of differential dose levels of methylxanthines on lactational performance, with respect to milk volume and composition and pup growth. the methylxanthines; caffeine, theophylline and theobromine, were administered via drinking water in the proportions occurring in tea, at a dose of 50, 1 and 2 mg/kg body-weight respectively to wistar albino rats throughout pregnancy and for up to the 14th day of lactation. a fourth group received a mixture of all t ... | 1990 | 2223739 |
| the effect of methylxanthines on milk volume and composition, and growth of rat pups. | a study was conducted to investigate the effect of differential dose levels of methylxanthines on lactational performance, with respect to milk volume and composition and pup growth. the methylxanthines; caffeine, theophylline and theobromine, were administered via drinking water in the proportions occurring in tea, at a dose of 50, 1 and 2 mg/kg body-weight respectively to wistar albino rats throughout pregnancy and for up to the 14th day of lactation. a fourth group received a mixture of all t ... | 1990 | 2223739 |
| tourette's disorder and associated complex behaviors: a case report. | a case of a man with tourette's disorder associated with obsessive-compulsive disorder, multiple sexual paraphilias, and aggressive behavior is described. treatment with haloperidol led to improvement in the characteristic tics of tourette's disorder as well as to improvement in these three complex-associated behaviors. after haloperidol was discontinued, an exacerbation of tics and the associated behaviors occurred. | 1990 | 2238716 |
| prevalence of helicobacter pylori in symptomatic patients undergoing gastrointestinal endoscopy. | | 1990 | 2258222 |
| prevalence of helicobacter pylori in symptomatic patients undergoing gastrointestinal endoscopy. | | 1990 | 2258222 |
| human keratinocytes are a source for tumor necrosis factor alpha: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. | tumor necrosis factor alpha (tnf-alpha), in addition to being cytotoxic for certain tumor cells, has turned out as a multifunctional cytokine that is involved in the regulation of immunity and inflammation. since human keratinocytes have been demonstrated to be a potent source of various cytokines, it was investigated whether epidermal cells synthesize and release tnf-alpha. supernatants derived from normal human keratinocytes (hnk) and human epidermoid carcinoma cell lines (kb, a431) were teste ... | 1990 | 2258696 |
| human keratinocytes are a source for tumor necrosis factor alpha: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. | tumor necrosis factor alpha (tnf-alpha), in addition to being cytotoxic for certain tumor cells, has turned out as a multifunctional cytokine that is involved in the regulation of immunity and inflammation. since human keratinocytes have been demonstrated to be a potent source of various cytokines, it was investigated whether epidermal cells synthesize and release tnf-alpha. supernatants derived from normal human keratinocytes (hnk) and human epidermoid carcinoma cell lines (kb, a431) were teste ... | 1990 | 2258696 |
| induction of carcinoembryonic-antigen-gene expression in human colorectal carcinoma by sodium butyrate. | the effect of sodium butyrate on the expression of the carcinoembryonic-antigen (cea) gene was studied in two poorly differentiated colorectal-carcinoma cell lines (clone-a and mip-101) and in one well-differentiated cell line (ls-174t); a.t.c.c. no. ccl 188). northern-blot and dot-blot analyses indicated a steady increase in cea mrna from day 4 to a maximal level by day 14 after these cells were exposed to 2 mm-sodium butyrate. studies using nuclear run-off assays followed by dot-blot hybridiza ... | 1990 | 2268282 |
| effect of phospholipids on purified lipoamidase. | phospholipids were added to purified lipoamidase from porcine brain microsomal membranes, and changes in lipoamidase activity were examined. approximately twofold activation of lipoamidase activity occurred upon the addition of phosphatidylethanolamine. on the other hand, phosphatidylserine, cardiolipin, and phosphatidic acid reduced the enzyme activity by approximately 80%. this pattern of the activation of lipoamidase by phosphatidylethanolamine and its inhibition by phosphatidylserine is simi ... | 1990 | 2347395 |
| atypical presentation of chancroid in a patient infected with the human immunodeficiency virus. | | 1990 | 2368772 |
| in situ assembly states of (na+,k+)-pump atpase in human erythrocytes. radiation target size analyses. | the in situ assembly state of the (na+,k+)-pump atpase of human erythrocytes was studied by applying the classical target theory to radiation inactivation data of the ouabain-sensitive sodium efflux and atp hydrolysis. erythrocytes and their extensively washed white ghosts were irradiated at -45 to -50 degrees c with an increasing dose of 1.5-mev electron beam, and after thawing, the na+-pump flux and/or enzyme activities were assayed. each activity measured was reduced as a simple exponential f ... | 1985 | 2414287 |
| tumor angiogenesis inhibition by prostaglandin synthetase inhibitors. | the release of prostaglandins from tumor cells seems to play an important role in tumor angiogenesis, in which platelet-derived factors may be also included. administration of prostaglandin synthetase inhibitors reduces the growth of both experimental and human malignant tumors. one explanation may be reduced tumor vascularization, as observed in microangiographic studies of experimental transplantable tumors. a similar effect was observed after induced thrombocytopenia. a number of angiogenesis ... | 1986 | 2423014 |
| [a study of the antineoplastic activity of k18]. | k18 is a new drug produced by a combination of melphalan and human immunoglobulin. k18 produced an inhibitory effect on the proliferation of human gastric and colon cancer transplanted in to nude mice. also, combination effects of k18 with mmc and 5-fu were recognized in the same system. in a study of distribution after k18 administration, the accumulation and retention of this drug in the tumor region were observed. in the case of melphalan administration, these phenomena were not observed. the ... | 1986 | 2423038 |
| cotranscription of the escherichia coli isoleucyl-trna synthetase (iles) and prolipoprotein signal peptidase (lsp) genes. fine-structure mapping of the lsp internal promoter. | we have applied the technique of northern blotting hybridization analysis to examine the transcription of the escherichia coli isoleucyl-trna synthetase (iles) and prolipoprotein signal peptidase (lsp) genes. rna samples from the wild-type strain cs412 and cs412 containing the plasmids pmt521 and psyc890 were examined with iles- and lsp-specific 32p-labeled probes. pmt521 is a pbr322 derivative into which a chromosomal dna fragment containing the iles and lsp genes has been cloned downstream of ... | 1987 | 2432063 |
| effect of gastroduodenostomy on intestinal vasoactive intestinal peptide (vip) levels, and vip binding and vip stimulation of cyclic amp in intestinal epithelial cells from rat. | the concentration of vip in duodenum and jejunum as well as the interaction of vip (binding and stimulation of cyclic amp accumulation) with epithelial cells from both gut segments were studied in rats after surgical bypass of the pylorus by gastroduodenostomy. duodenal vip concentration increased in rats with gastroduodenostomy as compared to sham-operated animals. the binding capacity (but not the affinity) of vip binding sites and the efficiency (but not the potency) of vip on cyclic amp accu ... | 1987 | 2440464 |
| synaptophysin expressed in the bronchopulmonary tract: neuroendocrine cells, neuroepithelial bodies, and neuroendocrine neoplasms. | synaptophysin is an integral membrane glycoprotein with an mr of 38,000 that occurs in the small, clear vesicles present in neuronal cells and tumors as well as in pancreatic islet cells and various neuroendocrine (ne) carcinomas. we found that synaptophysin is also expressed in normal ne cells of the lungs of newborn rabbits and mice as well as of human fetuses. in bronchial ganglion cells and in nerves, synaptophysin is coexpressed with neurofilament proteins (nfps), whereas in solitary ne cel ... | 1987 | 2442053 |
| human amnion cells: modulation of expression of specific antigens. | antigen expression by fetal cells near the mother is of interest because of the possibility that some antigens may stimulate beneficial maternal immune responses or may prevent the development of harmful responses. the recent generation of monoclonal antibodies to amnion and trophoblast antigens together with the finding that class i histocompatibility antigen (hla) expression by amnion cells can be manipulated provided an opportunity to determine if any relationships exist among expression of h ... | 1987 | 2442388 |
| human amnion cells: modulation of expression of specific antigens. | antigen expression by fetal cells near the mother is of interest because of the possibility that some antigens may stimulate beneficial maternal immune responses or may prevent the development of harmful responses. the recent generation of monoclonal antibodies to amnion and trophoblast antigens together with the finding that class i histocompatibility antigen (hla) expression by amnion cells can be manipulated provided an opportunity to determine if any relationships exist among expression of h ... | 1987 | 2442388 |
| [persistence of antibodies of the igg class against cytomegaloviruses in blood donors]. | high titre plasmas gained from blood donors are the initial material used for producing human immunoglobulin containing cytomegalovirus antibodies (cmv-hig). for this purpose the sera gained from 467 permanent donors at the district institute for blood and transfusion service in berlin were investigated on their cmv antibody content of igg class by means of an indirect immunofluorescence test (ift). the infection rate of blood donors amounted to 62% (291/467). cmv-igg titre greater than or equal ... | 1987 | 2446998 |
| [the effect of food consistency on conditioned avoidance response in mice and rats]. | the purpose of this study was to examine the effects of chewing a hard and soft diet on acquisition of conditioned avoidance response in mice and rats. 76 c57bl mice and 38 wistar rats were divided into 2 groups respectively 20 days after birth. one group was fed a solid diet and another was fed a powder diet containing the same components as the solid one (nihon crea company, ce-2). using the avoidance conditioning system of gunma university, conditioned avoidance experiments started at the age ... | 1989 | 2519255 |
| [expressions of 2h4 and 4b4 antigens on atl cells]. | we studied the expression of 2h4 and 4b4 on the surfaces of leukemia cells from 17 patients with adult t-cell leukemia (atl) as well as of cells belonging to 2 t-cell lines derived from atl patients. the effects of the supernatants obtained from culture fluids of the atl cells and the t-cell lines on igg production of a human b-cell line, cess cells, were also examined. on the surfaces of the atl cells from 15 out of 17 cases and of the cells of 2 t-cell lines 4b4 obviously existed at higher per ... | 1989 | 2528006 |
| diabetic state-induced activation of calcium-activated neutral proteinase in mouse skeletal muscle. | the effect of a diabetic state in the diabetic kk-cay mouse on calcium activated neutral proteinase (canp) of hind-limb skeletal muscles was investigated. in the diabetic state, there was an increased sensitivity to activation of canp by calcium (ca). in addition, there was an enhancement of maximal activity of the enzyme. the effect was induced by secondary modification of the diabetic state, but not genetical factors. several lines of evidence suggest that the canp is responsible for 92 k dalt ... | 1989 | 2561275 |
| changes in oligosaccharides of human blood cells measured by tritium- and fitc-labeled lectins after in vitro- and in vivo-irradiation--lectin binding as a 'biological dosemeter'? | the lectin-binding system has been described previously as a biological dosimeter, by revealing induced changes in oligosaccharides of the cell membrane. the measurements were performed by binding [3h]concanavalin a to blood cells. our results on human blood cells irradiated in vitro with doses in the range 0.5-5 gy indicate great difficulties in using radioactive labeled con-a for an accurate quantitative analysis of radiation effects on cell membranes. it appears nearly impossible to different ... | 1989 | 2565944 |
| distribution of calcitonin- and somatostatin-containing cells in thyroid lymphoma and in hashimoto's thyroiditis. | eleven cases of thyroid lymphoma were studied by the immunoperoxidase avidin-biotin technique with calcitonin and somatostatin rabbit antisera. in 6 cases of non-hodgkin lymphoma, in thyroid tissue residual to the lymphomatous infiltration, the c cell density was markedly increased and clustering was often observed; the c cells often took part in the follicular lining, frequently with polar distribution; these elements displayed a strong positivity for calcitonin, while the number of somatostati ... | 1989 | 2567176 |
| the effect of pinealectomy and jejunal loop diversion on rat small bowel crypt cell kinetics. | previously, it was found that diversion of an isolated loop of jejunum into the colon was associated with a significantly diminished crypt cell proliferation rate in the isolated loop, probably principally because of the diminished amount of nutrient available to the diverted mucosa. it has also been shown previously that removal of the pineal gland is associated with a considerable increase in the jejunal crypt cell mitotic rate. in the present investigation it was found that following pinealec ... | 1989 | 2570492 |
| functionally different htlv i-infected t cell lines with the same phenotype derived from a patient with melanoma. | two autologous t cell lines infected with htlv i are described. t cells from a patient with malignant melanoma were infected with htlv i by co-culture with a htlv i-producing t cell line, slb i. both t cell lines express identical phenotype (cd3+, cd4+, 4b4+, 2h4-) but they demonstrate marked differences in growth characteristics and function. one of these two lines, referred to as tftx, established from the autologous tumor activated peripheral blood lymphocytes (pbl), grows in culture without ... | 1989 | 2576655 |
| complex membrane transport systems. a non-markovian approach. | this paper suggests a method of how to deal with complex membrane transport systems such as ion channels or ion pumps formed by proteins. the complexity of these systems results from the fact that proteins may undergo an internal dynamics of conformational changes and may thereby affect the transmembrane transport. usually, complex transport systems are mapped into multi-state graphs and couched in terms of markovian master equations. it is shown in this paper how the dimensionality of such mult ... | 1985 | 2578832 |
| characterization of elevated plasma alkaline phosphatase activity in genetically obese mice. | alkaline phosphatase activity in obese mice (c57bl/6j ob/ob) was significantly increased from 18 to 63 weeks of age when compared to that of their lean controls (c57bl/6j +/?). in 5 week old animals, the earliest age examined, the circulating activity of alkaline phosphatase was similar in both obese mice and their lean counterparts. to characterize the circulating alkaline phosphatase activity in the obese mouse and its lean counterpart, the response of the enzyme to fasting, various inhibitors ... | 1985 | 2579311 |
| development of cellular polarity and tight junctions in parotid acinar cells of postnatal rat. | the morphogenesis of acino-tubular structures and cytodifferentiation of acinar cells in developing rat parotid glands from the day of birth to the 7th day after birth were studied by conventional ultrathin-section electron microscopy in conjunction with freeze replica and space tracer methods. an ultrathin-section study indicated that the acinar cells developed sequentially in the order of the following three stages: (1) the stage of undifferentiated cell immediately after birth, in which the p ... | 1989 | 2584909 |
| in vitro proliferative and cytotoxic responses of pbl from theileria annulata-immune cattle. | peripheral blood lymphocytes were isolated from healthy calves and were subsequently infected with sporozoites of theileria annulata in vitro. the infected cells were passaged for 50 times and thereafter inoculated into animals from which they were previously isolated. within 4-5 days, schizont-containing cells were demonstrable in the lymph nodes of all animals. few days later, merozoites were detected in erythrocytes. a slight decrease in the counts of lymphocytes and leucocytes was also found ... | 1989 | 2596199 |
| activation of human natural killer cells by lipopolysaccharide from actinobacillus actinomycetemcomitans. | lipopolysaccharide (lps) from the y4 strain of this bacterium, which is implicated in the pathogenesis of juvenile periodontitis, was incubated with human peripheral blood lymphocytes (pbl) and its action compared to that of lps from escherichia coli. both lps augmented cytotoxicity measured against natural killer (nk) cell-resistant tumour targets within 24 h of incubation. cytotoxicity was exclusively found in nk-enriched low-density large granular lymphocyte fractions, as separated by percoll ... | 1989 | 2597038 |
| a method for studying rat embryonic metabolism by microcannulation of the vitelline circulation. | a novel technique which involves the cannulation of the vitelline vessels of the 11.5 days rat embryo is described; this has allowed the introduction of both macromolecular and particulate substances directly into the embryo, thus bypassing the digestive and metabolic activity of the visceral yolk sac and facilitating the study of the fate of such substances within the embryo. the method does not affect the growth of the conceptus in vitro providing that inert solutions (e.g. azo blue dye in han ... | 1989 | 2606767 |
| the behaviour of sensory ganglia in relation to their environment. | the nodose ganglion was removed from rats and transplanted into the denervated tongue and into the denervated or innervated sternocleidomastoid muscle. in another procedure the ganglion was sutured to the distal hypoglossal or sciatic nerve. the ganglia failed to develop motor end-plates on denervated muscle. they did, however, induce formation of taste buds on the lingual epithelium. the regenerated nerve fibres ramified extensively within the connective tissue, invading perivascular tissue and ... | 1989 | 2606773 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis. | selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ... | 1989 | 2613803 |
| the rat 78,000 dalton glucose-regulated protein (grp78) as a precursor for the rat steroidogenesis-activator polypeptide (sap): the sap coding sequence is homologous with the terminal end of grp78. | based on the striking sequence identity between the amino acid sequence of rat steroidogenesis-activator polypeptide (sap) and the carboxyl terminus of the 78,000 dalton glucose-regulated protein (grp78), the precursor-product relationship between grp78 and sap was investigated in leydig cells. immunoblot analysis with peptide antibodies specific for grp78 and sap showed that the putative sap precursor is also immunoreactive with the anti-grp78 antibody. genomic blot hybridizations further revea ... | 1989 | 2628731 |
| long residual activity of bacillus sphaericus 1593 against culex quinquefasciatus larvae in artificial pools. | the microbial larvicide, bacillus sphaericus 1593, was evaluated against culex quinquefasciatus larvae in artificial pools at huay kwang area in bangkok between march and october 1985. this study was aimed at determining the residual activity of b. sphaericus 1593 in waste water under field conditions. the larvicidal activity of b. sphaericus 1593 was found to persist for at least 5 months in artificial pools containing waste water. the populations of b. sphaericus 1593 in the test water fluctua ... | 1989 | 2633350 |
| esculetin inhibits t cell activation without suppressing il-2 production or il-2 receptor expression. | esculetin (6,7-dihydroxycoumarin) was found to inhibit dose-dependently the proliferation of human t cells stimulated by pha or phorbolester plus ionomycin. proliferation in autologous and allogeneic mlr and generation of cytotoxic t cells under limiting dilution conditions were also suppressed, with more than 90% inhibition seen at 50 microm esculetin. the immunosuppressive effect of esculetin was not due to toxicity. esculetin did not inhibit interleukin-2 (il-2) production, nor did it interfe ... | 1989 | 2654016 |
| nasal obstruction and human communication. | nasal obstruction may cause a variety of communication disorders, particularly in children. the effects of nasal obstruction on hearing, speech, language, and voice are examined. methods for assessing the effects of nasal obstruction are delineated, and recommendations for therapeutic interventions are described. | 1989 | 2664661 |
| properties and incompatibility behavior of miniplasmids derived from the bireplicon plasmid pcg86. | many plasmids belonging to the f incompatibility groups contain more than one basic replicon. the chimeric plasmid pcg86 is an example of such a multireplicon plasmid. the two basic replicons of pcg86, repfiia/fic and repfib have been cloned and re-ligated, the copy numbers of the clones have been determined, and the incompatibility behavior of plasmids containing the ligated replicons and the individual replicons has been studied. the bireplicon plasmids are not expected to be incompatible as r ... | 1989 | 2674652 |
| [isolation of individual chromosomes and their use in studying the human and animal genome]. | this mini-review considers the main achievements in the field of human and animal chromosome separation: technical problems of flow-karyotyping and flow-sorting, the types of cell lines and chromosome source used, different protocols of chromosome suspension preparation, fluorochromes for chromosome staining, efficiency of chromosome sorting and purity of the fractions. the use of fractions for creating chromosome-specific human gene libraries and mapping cloned genes and dna fragment are also c ... | 1989 | 2685546 |
| [isolation of individual chromosomes and their use in studying the human and animal genome]. | this mini-review considers the main achievements in the field of human and animal chromosome separation: technical problems of flow-karyotyping and flow-sorting, the types of cell lines and chromosome source used, different protocols of chromosome suspension preparation, fluorochromes for chromosome staining, efficiency of chromosome sorting and purity of the fractions. the use of fractions for creating chromosome-specific human gene libraries and mapping cloned genes and dna fragment are also c ... | 1989 | 2685546 |
| transformation of oocysts from several coccidian species by heat treatment. | the transformation of sporocysts in oocysts of eimeria caviae, e. intestinalis, e. nieschulzi, e. separata, e. tenella, isospora canis, i. heydorni, and toxoplasma gondii by heat treatment was examined. fresh unsporulated oocysts from these species were heated at 50 degrees or 55 degrees c for less than 5 min and cultivated at 25 degrees c for 1 week. no transformation of sporocysts was observed in the sporulated oocysts following heating at 55 degrees c, but sporulated oocysts of tyzzeria and c ... | 1989 | 2704721 |
| a preliminary survey of parasitic infections and nutritional status among school children in riyadh, saudi arabia. | a total of 320 school children in the model institute of the capital in riyadh, were clinically and parasitologically examined. thirty-five (10.94%) had intestinal parasites. entamoeba coli (61.91%) was found more frequently among the commensals, while giardia lamblia (28.57%) was the most common pathogenic parasite. all urines were parasite free. the low infection rate with gardia could be explained by the good physical and nutritional status and better sanitary and living conditions. | 1989 | 2708849 |
| scanning electron microscopy of very small fat cells and mature fat cells in human obesity. | to determine the effect of obesity on the size distribution of fat cell populations in human adipose tissue, omental fat tissue biopsies were obtained from lean, moderately obese, and massively obese patients. the size distributions of adipocytes from lean and obese fat tissues examined by the scanning electron microscopic method were bimodal, consisting of populations of very small fat cells and mature fat cells, in contrast to collagenase-derived isolated cells that showed only the large matur ... | 1989 | 2715732 |
| purification of alpha-ketoaldehyde dehydrogenase from the human liver and its possible significance in the control of glycation. | alfa-ketoaldehyde dehydrogenase, which was extracted and purified from human livers, may act on carbonyl compounds, such as 3-deoxyglucosone, and be involved in the control of glycation (maillard reaction) in the body. | 1989 | 2721637 |
| regulation of hepatic cortisol sulfotransferase in rats by pituitary growth hormone. | cytosolic sulfating activities of 4-pregnen-11 beta, 17 alpha, 21-triol-3,20-dione (cortisol) to the 21-sulfate were 4 to 5 times higher in livers of female than male adult rats. the activity was decreased by administration of testosterone propionate (tp) to ovariectomized, but not to intact, female rats. in male rats, the rate of cortisol sulfation was elevated by neonatal castration and was restored in part by the administration of tp to the castrated rats. in addition, the sulfating activity ... | 1989 | 2725475 |
| comparative study on breast milk of mothers delivering preterm and term infants--protein, fat and lactose. | milk samples were collected from 35 mothers delivering preterm infants and from 35 mothers delivering term infants at 3 time intervals over the first 3 weeks of lactation. the samples were collected by manual expression from both breasts into polyethylene containers and were frozen at -20 degrees c and stored for analysis. the protein content of preterm milks during the first and second weeks is higher than that of term milk (p less than 0.05). the fat and lactose contents during the first week ... | 1989 | 2725632 |
| gangrenous ergotism in a suckler herd. | | 1989 | 2728284 |
| internal screw fixation: comparison of placement pattern and rigidity. | multiple screw placement patterns and techniques have been described for fixation of the sagittal split ramus osteotomy. this study evaluated transverse strength (rigidity) of six groups of simulated sagittal osteotomies fixed with different screw patterns and placement techniques. osteotomies fixed with screws placed in an inverted l pattern were significantly more rigid than those fixed with screws placed in a linear pattern or k-wires placed in a triangular pattern (p less than .05). no signi ... | 1989 | 2732831 |
| cloning and characterization of bovine cytochrome p-450(11 beta) genes. | we isolated 4 different clones of the p-450(11 beta) gene from a bovine genomic library. these genomic clones were highly homologous with each other. two of the isolated clones were pseudogenes. determination of its nucleotide sequences indicated that the bovine p-450(11 beta) gene is divided into 9 exons by 8 introns and that it is about 8.5 kb in total length. the number of exons and the locations of intron insertion into the p-450(11 beta) gene are identical with those in the case of p-450(sc ... | 1989 | 2753866 |
| decreased 3 alpha-androstanediol glucuronide levels in plasma and random urines in male pseudohermaphroditism caused by 5 alpha-reductase deficiency. | concentrations of 3 alpha-diol glucuronide (3 alpha-diol g) in plasma and/or random urine samples were determined in seven subjects with familial male pseudohermaphroditism (fmp) due to 5 alpha-reductase deficiency (5 alpha-rd). all subjects were natives of an isolated turkish village with a high incidence of consanguineous marriage. a specific and sensitive antibody to 3 alpha-diol was used for radioimmunoassay of 3 alpha-diol g after hydrolysis and chromatographic purification. the mean plasma ... | 1989 | 2770532 |
| combined gastric pull-up and microvascular jejunal transfer procedure after pharyngolaryngoesophagectomy. | a case of simultaneous carcinomas of the lower thoracic esophagus and the oropharynx in a 62-year-old man is reported. reconstruction was successfully performed using a one-stage operation consisting of pharyngolaryngoesophagectomy followed by a combination of gastric pull-up and microvascular jejunal transfer procedures. the postoperative course was uneventful except for regurgitation of food, which persisted for 1 month. | 1989 | 2774731 |
| effect of ethanol on hepatotoxicity of acetaminophen in mice and on reactive metabolite formation by mouse and human liver microsomes. | the protective effect of a single dose of ethanol with regard to hepatotoxicity caused by acetaminophen (apap) can be a consequence of either direct or indirect inhibition of apap oxidation to its hepatotoxic intermediate (n-acetyl-p-benzoquinoneimine, napqi), or augmentation of repair mechanisms following the hepatotoxic insult. the mechanism of hepatoprotection appears to be species dependent. by varying the time of ethanol administration relative to apap in mice (30 min before to 240 min afte ... | 1989 | 2781565 |
| mechanism of action of human pancreatic and salivary alpha-amylase on 4,6-ethylidene-alpha-4-nitrophenyl-maltoheptaoside substrate. | | 1989 | 2783397 |
| mechanism of action of human pancreatic and salivary alpha-amylase on 4,6-ethylidene-alpha-4-nitrophenyl-maltoheptaoside substrate. | | 1989 | 2783397 |