| purification and regulation of glutamine synthetase in a collagenolytic vibrio alginolyticus strain. | glutamine synthetase (ec 6.3.1.2) has been purified from a collagenolytic vibrio alginolyticus strain. the apparent molecular weight of the glutamine synthetase subunit was approximately 62,000. this indicates a particle weight for the undissociated enzyme of 744,000, assuming the enzyme is the typical dodecamer. the glutamine synthetase enzyme had a sedimentation coefficient of 25.9 s and seems to be regulated by adenylylation and deadenylylation. the ph profiles assayed by the gamma-glutamyltr ... | 1985 | 2859007 |
| nucleotide sequence of the vibrio alginolyticus calcium-dependent, detergent-resistant alkaline serine exoprotease a. | the nucleotide sequence of the vibrio alginolyticus alkaline serine exoprotease a (proa) gene cloned in escherichia coli was determined. the exoprotease a gene (proa) consisted of 1602 bp which encoded a protein of 534 amino acids (aa) with an mr of 55,900. the region upstream from the gene was characterized by a putative promoter consensus region (-10 -35), a ribosome-binding site and atg start codon. the proa gene encodes a typical 21-aa n-terminal signal sequence which, when fused to alkaline ... | 1989 | 2546861 |
| membrane-linked energy transductions. bioenergetic functions of sodium: h+ is not unique as a coupling ion. | the concept is developed according to which na+, like h+, can play the role of a coupling ion in energy-transducing biomembranes. this idea is based on observations that (i) na+ can be extruded from the cell by primary pumps (na-motive nadh-quinone reductase, decarboxylase or atpase), and (ii) the downhill na+ flux into the cell can be coupled with the performance of all the three types of membrane-linked work i.e. chemical (atp synthesis), osmotic (accumulation of solutes) and mechanical (motil ... | 1985 | 2863140 |
| [humoral factors of local and systemic immunity in the multiple peroral administration of a corpuscular pertussis vaccine]. | multiple oral immunization with pertussis corpuscular vaccine was shown to lead to the considerable stimulation of local and systemic humoral immunity. the data on the titers of specific and normal secretory antibodies, on the levels of iga in washings from the oral cavity, the small intestine and the lungs, on the titers of agglutinins and hemagglutinins in the blood serum, as well as on the morpho-functional transformation of the mucous membrane and the associated lymphoid tissue in the digest ... | 1985 | 2868593 |
| [humoral factors of local and systemic immunity in the multiple peroral administration of a corpuscular pertussis vaccine]. | multiple oral immunization with pertussis corpuscular vaccine was shown to lead to the considerable stimulation of local and systemic humoral immunity. the data on the titers of specific and normal secretory antibodies, on the levels of iga in washings from the oral cavity, the small intestine and the lungs, on the titers of agglutinins and hemagglutinins in the blood serum, as well as on the morpho-functional transformation of the mucous membrane and the associated lymphoid tissue in the digest ... | 1985 | 2868593 |
| radioactivity in breast milk after chernobyl. | | 1986 | 2872450 |
| nucleotide sequence and analysis of the vibrio alginolyticus sucrase gene (scrb). | the nucleotide sequence of a 2.119-kb dna fragment containing the vibrio alginolyticus sucrase gene (scrb) was determined. the complete sequence (484 aa residues) of the sucrase was deduced and homology was detected between the sucrase enzymes from v. alginolyticus and the gram-positive bacteria bacillus subtilis and streptococcus mutans. in escherichia coli cells the cloned v. alginolyticus sucrase is translocated to the periplasm. transposon phoa mutagenesis experiments strongly suggested that ... | 1989 | 2551785 |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| inadequate blockade by hexamethonium of the baroreceptor heart rate response in anesthetized and conscious rats. | the ability of hexamethonium (hex) alone or in combination with atropine methylbromide (amb) to block the heart rate (hr) response to baroreceptor activation by phenylephrine (pe) or angiotensin ii (aii)-evoked increments in blood pressure was evaluated in rats. a highly significant negative correlation existed under control conditions between the changes in map and hr. hex (10-20 mg/kg) did not influence the baroreceptor hr response of anesthetized and conscious rats, suggesting anesthesia was ... | 1989 | 2567154 |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| nucleotide sequence of the vibrio alginolyticus glna region. | the nucleotide sequence of a 4 kb fragment containing the vibrio alginolyticus glna, ntrb and ntrc genes was determined. the upstream region of the glna gene contained tandem promoters. the upstream promoter resembled the consensus sequence for escherichia coli sigma 70 promoters whereas the presumptive downstream promoter showed homology with nitrogen regulated promoters. four putative nri binding sites were located between the tandem promoters. the ntrb gene was preceded by a single presumptiv ... | 1989 | 2574025 |
| improved fluorogenic assay for rapid detection of vibrio parahaemolyticus in foods. | an improved fluorogenic assay for the rapid detection of vibrio parahaemolyticus was developed. in the improved assay, the enrichment of v. parahaemolyticus was carried out in arabinose-glucuronate medium (0.5% arabinose, 0.25% glucuronate, 0.1% polypeptone, 0.1% yeast extract, 0.1% ammonium sulfate, 2% nacl, 2 micrograms of polymyxin b sulfate per ml, ph 8.5) at 37 degrees c. after the cultivation, the trypsinlike activity of the bacteria was measured by fluorescence with the fluorogenic substr ... | 1990 | 2339897 |
| purification and some properties of a novel l-2,4-diaminobutyric acid decarboxylase from vibrio alginolyticus. | previous investigations have shown that members of the genus vibrio possess a novel enzyme activity decarboxylating l-2,4-diaminobutyric acid (daba) to 1,3-diaminopropane (dap). in this paper we describe the purification, by about 3600-fold, of the enzyme from v. alginolyticus. the purified enzyme was apparently homogeneous, and had a specific activity of 4200 nmol dap min-1 (mg protein)-1. the enzyme protein has an mr of 450,000 +/- 20,000 and is apparently comprised of four identical subunits ... | 1989 | 2614383 |
| trends in public knowledge and attitudes about aids, south carolina, 1987-1988. | the south carolina department of health and environmental control aids program assessed the state population's knowledge and attitudes about aids and hiv transmission in 1987 and 1988. each year approximately 1,800 adults were selected by a random 3-stage cluster design and asked seventeen questions by telephone about aids and hiv. questions addressed attitudes, general knowledge, hiv transmission by casual contact, hiv transmission by sex and iv drug contact, and hiv transmission by blood donat ... | 1989 | 2615326 |
| [determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method]. | use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ... | 1985 | 2578835 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| generation of the electrochemical potential of na+ by the na+-motive nadh oxidase in inverted membrane vesicles of vibrio alginolyticus. | inverted membrane vesicles prepared from vibrio alginolyticus generated a membrane potential (positive inside) and accumulated na+ by the oxidation of nadh. generation of the membrane potential required na+ and was inhibited by 2-heptyl-4-hydroxyquinoline n-oxide, a specific inhibitor of the na+-dependent nadh oxidase. collapse of the membrane potential by valinomycin stimulated the uptake of na+. in contrast, accumulation of h+ was not detected under all the conditions tested. these results sug ... | 1985 | 2579856 |
| cardiopulmonary baroreflex control of brachial artery diameter in sustained essential hypertension. | an exaggerated reflex response of forearm resistance vessels following stimulation of cardiac mechanoreceptors has been observed in human hypertension. in the present study we analyze the possibility that such an increased response also involves the large brachial artery. for that purpose, the influence of leg elevation in subjects in a supine position on brachial artery diameter and blood flow velocity was studied in 12 healthy, normal males and 17 males of the same age with sustained essential ... | 1989 | 2607141 |
| blood lipid variability in relation to relative weight and biochemical markers of tobacco and alcohol consumption. | carboxyhaemoglobin (cohb%) and gamma-glutamyl-transferase (ggt) are today frequently used as objective indicators of tobacco and alcohol consumption. the relationships between cohb%, ggt and relative body weight, cholesterol, triglyceride and apolipoprotein ai (apo-ai) were studied in middle-aged men attending a preventive medical programme in malmö, sweden. although statistically significant the influence of cohb% on cholesterol and triglyceride was found to be clinically insignificant. ggt and ... | 1985 | 2861602 |
| plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates. | a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ... | 1985 | 3010836 |
| temperature and oxygen regulated expression of a glutamine synthetase gene from vibrio alginolyticus cloned in escherichia coli. | glutamine synthetase (gs) synthesis in vibrio alginolyticus was regulated by temperature, oxygen and nitrogen levels. a gs gene, glna from v. alginolyticus was cloned on a 5.67 kb insert in the recombinant plasmid prm210, which enabled escherichia coli glna, ntrb, ntrc deletion mutants to utilize (nh4)2so4 as a sole source of nitrogen. the v. alginolyticus glna gene was expressed from a regulatory region contained within the cloned fragment. v. alginolyticus glna expression from prm210 was subje ... | 1986 | 2880573 |
| evidence of functional alpha 2-adrenergic receptors in adult-rat adipocytes by using the agonist uk 14304. | the aim of this study was to re-assess whether alpha 2-adrenergic receptors were present in rat adipocytes, by using uk 14304, a new and very selective alpha 2-agonist. the following observations demonstrate the presence of functional alpha 2-adrenoceptors in rat adipocytes. (1) adipocyte lipolysis was dose-dependently inhibited by uk 14304 (maximal effect 80% at 1 microm-uk 14304, 45% at 10 microm-uk 14304, under basal or theophylline-stimulated conditions respectively). (2) uk 14304 bound spec ... | 1988 | 2901830 |
| renal metabolism of the oxidized form of ascorbic acid (dehydro-l-ascorbic acid). | we evaluated whether specific transport and metabolic properties exist in rat and guinea pig kidney for handling the immediate oxidative product of ascorbic acid, dehydro-l-ascorbic acid. isolated tubules were used to measure uptake of 10 microm [14c]-dehydro-l-ascorbic acid over an 8-min incubation period. uptake did not show dependence on the bathing media electrolyte composition but was inhibited to some extent by glucose. in tubules of both animal species the majority of 14c label present in ... | 1989 | 2912166 |
| [differential diagnosis of viral diarrheas in calves]. | there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool. | 1986 | 3020762 |
| [differential diagnosis of viral diarrheas in calves]. | there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool. | 1986 | 3020762 |
| preventing second-generation complications due to staphylococcus aureus. | | 1989 | 2919929 |
| sympathetically-induced development of tension in jaw muscles: the possible contraction of intrafusal muscle fibres. | in rabbits, cats and rats anaesthetized, curarized, with the skull fixed in a stereotaxic apparatus, the peripheral stump of the cervical sympathetic nerve (c.s.n.) was electrically stimulated at frequencies within the physiological range and the isometric tension was recorded at the lower jaw. in a group of experiments the afferent discharge from the jaw elevator muscle spindles was also recorded, in the mesencephalic nucleus of the fifth cranial nerve. unilateral stimulation of the c.s.n. indu ... | 1985 | 2934686 |
| differential depression of hypoglossal nerve activity by alcohol. protection by pretreatment with medroxyprogesterone acetate. | upper airway obstruction during sleep occurs more commonly in men than in women and has been treated with progestational agents with some success. alcohol ingestion exacerbates sleep apnea, and recent studies have established that alcohol depresses the respiratory motor activity to upper airway muscles more than that to the diaphragm, a response pattern that favors upper airway obstruction during inspiration. to investigate the possibility that progesterone provides some protection from this act ... | 1986 | 2935056 |
| molecular diagnosis of human cancer. | | 1989 | 2677520 |
| the sodium cycle. ii. na+-coupled oxidative phosphorylation in vibrio alginolyticus cells. | the role of na+ in vibrio alginolyticus oxidative phosphorylation has been studied. it has been found that the addition of a respiratory substrate, lactate, to bacterial cells exhausted in endogenous pools of substrates and atp has a strong stimulating effect on oxygen consumption and atp synthesis. phosphorylation is found to be sensitive to anaerobiosis as well as to hqno, an agent inhibiting the na+-motive respiratory chain of v. alginolyticus. na+ loaded cells incubated in a k+ or li+ medium ... | 1986 | 2942186 |
| the atp-driven primary na+ pump in subcellular vesicles of vibrio alginolyticus. | subcellular vesicles of vibrio alginolyticus hydrolyze atp and accumulate na+ in an atp-dependent fashion. the na+ uptake is (i) strongly stimulated by delta psi-discharging agents, i.e., the protonophorous uncoupler cccp or valinomycin + k+ and (ii) arrested by dccd at a concentration strongly inhibiting atp hydrolysis. lower concentrations of dccd stimulate the na+ accumulation supported by atp hydrolysis as well as by nadh oxidation. it is concluded that there is an electrogenic dccd-sensitiv ... | 1988 | 2968282 |
| expression and regulation of a vibrio alginolyticus sucrose utilization system cloned in escherichia coli. | a halotolerant collagenolytic vibrio alginolyticus strain isolated from salted hides had intracellular sucrase activity and did not secret sucrase into the medium. the strain actively transported sucrose by a sucrose-inducible, na+-independent process. a 10.4-kilobase dna fragment of v. alginolyticus dna was cloned into escherichia coli. the recombinant e. coli(pvs100) could utilize sucrose as a sole carbon source. in contrast to v. alginolyticus, the recombinant e. coli produced both intra- and ... | 1987 | 3034863 |
| respiratory na+ pump and na+-dependent energetics in vibrio alginolyticus. | the marine bacterium vibrio alginolyticus was found to possess the respiratory na+ pump that generates an electrochemical potential of na+, which plays a central role in bioenergetics of v. alginolyticus, as a direct result of respiration. mutants defective in the na+ pump revealed that one of the two kinds of nadh: quinone oxidoreductase requires na+ for activity and functions as the na+ pump. the na+ pump composed of three subunits was purified and reconstituted into liposomes. generation of m ... | 1989 | 2687261 |
| oxidative metabolism of neonatal and adult rabbit lung macrophages stimulated with opsonized group b streptococci. | we compared the oxidative metabolism of alveolar macrophages (am) from adult and neonatal (1- and 7-day-old) rabbits before and after their in vitro exposure to type ia group b streptococci (gbs) opsonized with immune rabbit serum. nonstimulated am from 1-day-old, 7-day-old, or adult rabbits consumed o2 at a rate of 17 to 20 nmol/10(6) am per 10 min under basal conditions and released minimal amounts of superoxide (o2-) into the medium. approximately 80% of this basal respiration was of mitochon ... | 1985 | 2981197 |
| [existence of an additional structural component in the basal bodies of escherichia coli and vibrio alginolyticus flagellae]. | the structure of escherichia coli and vibrio alginolyticus flagella was studied using electron microscopy. an additional protein structure was shown to exist in the basal bodies of intact flagella in these organisms. it is possible that this structure involves three proteins important for the assembly of flagella, energy transduction, and a change-over in the direction of flagellar rotation. | 1989 | 2695799 |
| quantitative analysis of labelled inner retinal proteins in experimental optic neuritis. | in order to determine if axonal transport changes in chronic experimental allergic encephalomyelitis (eae) were due to blockade or increased discharge of fast transported proteins from the inner retina, we examined the presence of pulse labeled proteins in autoradiograms of the optic nerve head, retinal ganglion cell and nerve fiber layers of juvenile strain-13 guinea pigs with chronic eae and normal controls. quantitative analysis of silver grains, performed six and twenty-four hours following ... | 1989 | 2707041 |
| vibrio parahaemolyticus and other halophilic vibrios associated with seafood in hong kong. | the summer prevalence of vibrio parahaemolyticus and other halophilic vibrios in seafood from hong kong markets was investigated. halophilic vibrios were isolated from all seven types of seafood examined, and comprised 9.1%, 8% and 6.1% of contaminating aerobic heterotrophic bacteria from mussels, clams and oysters respectively. sucrose-positive vibrios were more common than sucrose-negative varieties. vibrio alginolyticus was the most frequently isolated species, followed by v. parahaemolyticus ... | 1989 | 2722715 |
| [the first isolation of vibrio alginolyticus from samples which caused food poisoning]. | we report an event of food poisoning traced to eating salted shrimps. vibrio alginolyticus was shown to be the causative agent through epidemiological investigations and etiological tests. vibro alginolyticus can bring about human septicaemia and wound infection and it was found in the feces of patients with diarrhea, but no determination on its pathogenicity was done. from the samples of food which led to food poisoning. vibrio alginolyticus was isolated, and for the first time it was determine ... | 1989 | 2737046 |
| ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart. | cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ... | 1989 | 2738265 |
| second-generation cephalosporins. | | 1986 | 3081544 |
| [development of a latex agglutination method for the diagnosis of pseudomonas aeruginosa infection]. | the present investigation has revealed the possibility of using different kinds of monodispersed polystyrene latex, produced in the ussr, as carriers in the process of the preparation of antibody diagnosticums intended for the detection of water-soluble slime antigens of pseudomonas aeruginosa strains belonging to the most widespread serological types. the optimum conditions for the preparation of latex reagents and for making the latex-agglutination test have been experimentally established. th ... | 1986 | 3087117 |
| terminal field of cholecystokinin-8-like immunoreactive projection neurons of the rat main olfactory bulb. | the terminal field of cholecystokinin-8 (cck)-like immunoreactive (cck-ir) tufted cells in the rat main olfactory bulb was examined by means of immunohistochemistry combined with either an anterograde tracer or a degeneration method. cck immunostaining was carried out in animals in which phaseolus vulgaris agglutinin (pha) had been injected into the main olfactory bulb. pairs of adjacent sections were processed for cck and pha immunostaining, respectively. dense cck-ir terminallike staining was ... | 1989 | 2754048 |
| [the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro]. | | 1989 | 2806660 |
| [role of virus-induced autoreactive t-lymphocytes, t-suppressors and the serum factor regulating their activity in the pathogenesis of experimental infection caused by the langat virus in mice]. | the precursors of autoreactive t-lymphocytes (partl) have been detected in the spleen of mice infected with langat virus. when introduced into syngeneic recipients, partl differentiate in their lymph nodes into autoreactive t-lymphocytes (artl) causing a fatal autoimmune disease in the syngeneic recipients in vivo and capable of destroying syngeneic cell cultures in vitro. in the thymus of mice infected with langat virus t-suppressors (ts) inhibiting the differentiation of partl into artl have b ... | 1985 | 2998127 |
| isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster. | previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ... | 1987 | 2820958 |
| isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster. | previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ... | 1987 | 2820958 |
| prevention of respiratory distress syndrome. | rds continues to be a major problem for premature infants despite a better understanding of its pathophysiology and of ways to try to prevent it. to date, prenatal administration of glucocorticoids has been the most widely used method of accelerating fetal lung development. however, several limitations of this therapy have prompted the search for alternative approaches. most efforts have focused on the potential use of combined hormonal therapy with glucocorticoids and either thyroid hormones or ... | 1988 | 3146813 |
| [transperitoneal transport of methotrexate: in vitro studies]. | | 1988 | 3147495 |
| [adhesion of fungi of the genus candida to vaginal epithelium in various physiological changes in the levels of sex hormones]. | | 1989 | 2655489 |
| eeg interaction between delta 8-tetrahydrocannabinol and some sedative-anxiolytic drugs. is the anxiogenic effect of cannabis related to an action on the gabaergic system? | | 1986 | 3017070 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| enhanced expression of the c-myc gene in bovine leukemia virus-induced bovine tumors. | we have detected elevated levels of c-myc gene expression in neoplastic cells from all seven bovine leukemia virus (blv)-induced bovine tumors examined, but not in blv-infected, nonneoplastic lymphoid cells. no rearrangement or amplification of the c-myc gene could be demonstrated in any of the blv-induced tumors. furthermore, blv proviral dna was found to have no preferred site of integration in these tumors. the possible mechanisms of enhanced expression of the c-myc gene in blv-induced tumors ... | 1986 | 3022917 |
| purification and characterization of a streptomycete collagenase. | a soil streptomycete designated as streptomyces sp. a8 produced an extracellular collagen hydrolysing enzyme that appeared to be 'true collagenase' as it degraded native collagen under physiological conditions and cleaved the synthetic hexapeptide 4-phenylazobenzyloxycarbonyl-l-prolyl-l-leucyl-glycyl-l-prolyl-d-a rginine into two tripeptides. the enzyme was purified by diethyl aminoethyl cellulose chromatography and sephadex g-150 gel filtration. the purified enzyme had an apparent molecular wei ... | 1986 | 3023269 |
| seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus). | the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ... | 1987 | 3030193 |
| [local ultrastructural characteristics of the intracerebral arteries of human fetuses]. | in 36 human fetuses (16 to 36 weeks of gestation) the ultrastructure of arterial vessels was investigated in the occipital and parietal hemispheres and midbrain tegmentum. during the antenatal ontogenesis the local features of the vessel wall structure were found, especially in the muscular layer. as it remained more differentiated in midbrain than in forebrain up to the end of gestation, more frequent forebrain stroke localization in infancy seems structurally dependent. | 1988 | 3213332 |
| seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus). | the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ... | 1987 | 3030193 |
| evolution of localization of reactions of adenosine triphosphatase (mg++-atp-ase), 5'nucleotidase (5'nt), alkaline phosphatase (ap), and acid phosphatase (acp) in developing rat testis. ii. after cdcl2 treatment. | the described experiments show the influence of a single dose of cadmium chloride (1.5 mg cdcl2/kg body weight applied intraperitoneally) on development of the male gonads from the 1st d of post-fetal life to 1.5 a of life. in the case of the enzymes triggering transportation processes, adenosine triphosphatase stimulated by mg++ (mg++-atp-ase), alkaline phosphatase (ap), and 5'nucleotidase (5'nt), a considerable damages begin to appear in the 15th d of life whereas in the case of acid phosphata ... | 1987 | 3031915 |
| [kidney morphology in hemolytic-uremic syndrome]. | morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ... | 1988 | 3233069 |
| role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats. | permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ... | 1987 | 3032567 |
| expression and secretion in yeast of a 400-kda envelope glycoprotein derived from epstein-barr virus. | the major envelope glycoprotein (gp350) of epstein-barr virus has been expressed and secreted in the yeast saccharomyces cerevisiae as a 400-kda glycoprotein. this is the first example of the secretion of such a large, heavily glycosylated heterologous protein in yeast. since gp350 proved highly toxic to s. cerevisiae, initial cellular growth required repression of the expression of gp350. using temperature- or galactose-inducible promoters, cells could be grown and the expression of gp350 then ... | 1987 | 3038696 |
| supplemented schneider's drosophila medium for isolation of leishmania donovani promastigotes. | | 1988 | 3252764 |
| the effects of in vivo antibiotics on neutrophil (pmn) activity in rabbits with peritonitis. | antibiotics play an important role in helping the host fight infection; however, the direct cellular effect of antibiotics on polymorphonuclear cells remains undefined. adherence, chemotaxis, phagocytosis, and superoxide anion production are important steps in the cascade of events initiated by the polymorphonucleocyte in bacterial killing. previous studies have shown inhibition as well as stimulation of neutrophil antibacterial therapy by antibiotics. peritoneal and blood polymorphonuclear neut ... | 1987 | 3041106 |
| sensitivity of some marine bacteria, a moderate halophile, and escherichia coli to uncouplers at alkaline ph. | the inhibitory effects of uncouplers on amino acid transport into three marine bacteria, vibrio alginolyticus 118, vibrio parahaemolyticus 113, and alteromonas haloplanktis 214, into a moderate halophile, vibrio costicola nrc 37001, and into escherichia coli k-12 were found to vary depending upon the uncoupler tested, its concentration, and the ph. higher concentrations of all of the uncouplers were required to inhibit transport at ph 8.5 than at ph 7.0. the protonophore carbonyl cyanide m-chlor ... | 1988 | 3045092 |
| similarity between active and trypsin-activated inactive renin in dog plasma by means of renin inhibition: the dog as an animal model for studies of inactive renin. | a method for trypsin-activation of dog plasma inactive renin is described. liquid phase trypsin (final concentration 6.7 mg/ml) was used and the reaction was stopped after 2 min at 4 degrees c by soybean trypsin inhibitor (13 mg/ml). renin was measured as angiotensin i (ang i) generation in trypsin-treated and untreated plasma using the antibody-trapping method, in the presence of excess ox renin substrate. the renin-like activity after trypsin was indeed due to renin, since ang i generation in ... | 1988 | 3065412 |
| [use of methylumbelliferylglucuronide (mug) for the rapid identification of e. coli in food microbiology]. | | 1988 | 3072974 |
| endothelium-dependent responses in human blood vessels. | | 1988 | 3073550 |
| endothelium-dependent responses in human blood vessels. | | 1988 | 3073550 |
| [antimicrobial susceptibility of vibrio parahaemolyticus and vibrio alginolyticus isolated from human feces and foods]. | | 1987 | 3106535 |
| biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes. | platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ... | 1987 | 3108266 |
| cytopathic effects of the pathogenic neisseria. studies using human fallopian tube organ cultures and human nasopharyngeal organ cultures. | infection of mucosal surfaces by n. gonorrhoeae and n. meningitidis may result in inflammation indicating potential injury to host cells. we used human fallopian tube organ cultures (ftoc) and human nasopharyngeal organ cultures (npoc) to study the mechanisms by which gonococci and meningococci damage human mucosal surfaces. early in the course of ftoc infected with gonococci and npoc infected with meningococci, damage was most apparent to ciliary activity. loss of ciliary activity was accompani ... | 1987 | 3130794 |
| bone mineral metabolism in experimental diabetes mellitus: osteocalcin as a measure of bone remodeling. | the etiology of diabetic osteopenia has not been established. the value of serum osteocalcin (bgp) as a marker of the bone abnormalities and the possible role of the polyol pathway in diabetic osteopenia were investigated. three groups of rats were studied over 7 weeks: group d (n = 12), rats with streptozotocin (55 mg/kg)-induced diabetes given saline by gavage; group ds (n = 12), rats with streptozotocin-induced diabetes given the aldose reductase inhibitor sorbinil (25 mg/kg) daily by gavage; ... | 1988 | 3133194 |
| vibrio alginolyticus and diarrhoeal disease. | | 1987 | 3429796 |
| a competitive enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in serum and urine samples. | in this solid-phase competitive enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in serum or urine, antiserum to human alpha 1-acid glycoprotein is incubated with solid-phase-bound alpha 1-acid glycoprotein in the presence of standard or sample. incubation with second antibody labeled with alkaline phosphatase then follows, before development with substrate. results obtained correlate well with a fluorescent assay involving the dye auramine o (r = 0.953) and with radial immunodiff ... | 1988 | 3135959 |
| generation of na+ electrochemical potential by the na+-motive nadh oxidase and na+/h+ antiport system of a moderately halophilic vibrio costicola. | cells of vibrio costicola at ph 8.5 generate both membrane potential (inside negative) and delta ph (inside acidic) in the presence of a proton conductor, carbonyl cyanide m-chlorophenylhydrazone (cccp). the generation of cccp-resistant membrane potential was inhibited by 2-heptyl-4-hydroxyquinoline-n-oxide that is known to inhibit the na+-motive nadh oxidase of vibrio alginolyticus. nadh oxidase, but not lactate oxidase, of inverted membrane vesicles prepared from v. costicola required na+ for ... | 1986 | 3005258 |
| deletion mutants that affect expression of epstein-barr virus nuclear antigen in cos-1 cells after gene transfer with simian virus 40 vectors containing portions of the bamhi k fragment. | we have identified sequences that affect the efficient expression of epstein-barr virus nuclear antigen (ebna 1) when the structural portion of its gene, found within the 2.9-kilobase-pair bamhi/hindiii fragment called ilf, is expressed from a simian virus 40 vector. a set of nested deletions at the bamhi end of the fragment was constructed by using bal 31 digestion, the addition of linkers, and ligation into psvod. the mutants were tested for their ability to express antigen in cos-1 monkey cel ... | 1986 | 3009849 |
| structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands. | continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ... | 1988 | 3165262 |
| nucleotide sequence of the regulatory gene xylr of the tol plasmid from pseudomonas putida. | we have determined the nucleotide sequence of the xylr gene for a transcriptional activator for the degradative pathway of aromatic hydrocarbons on the tol plasmid from pseudomonas putida. the 1698-bp sequence for a 566-amino acid (aa) protein (mr 63741) was identified as the xylr-encoding sequence. three regions in xylr show homology to klebsiella pneumoniae ntrc and nifa, both of which are transcriptional activators for the ntr and nif genes involved in the nitrogen metabolism. the central reg ... | 1988 | 3169574 |
| roles of the respiratory na+ pump in bioenergetics of vibrio alginolyticus. | bioenergetic characteristics of na+ pump-defective mutants of a marine bacterium vibrio alginolyticus were compared with those of the wild type and revertant. generation of membrane potential and motility at ph 8.5 in the mutants were completely inhibited by a proton conductor, carbonylcyanide m-chlorophenylhydrazone, whereas those in the wild type or revertant were resistant to the inhibitor. motility and amino acid transport were driven by the electrochemical potential of na+ not only in the w ... | 1988 | 3170506 |
| extensive deletions and insertions in different mhc supratypes detected by pusled field gel electrophoresis. | the genomic organization of the human mhc was examined in multiple examples of six different supratypes using pulsed field electrophoresis (pfge) after digestion of genomic dna with infrequency cutting restriction endonucleases. differences in restriction fragment length and band intensity were shown to be specific for each supratype. mapping of the mhc revealed that each supratype contains previously undescribed deletions and insertions between hla b and dq regions. | 1988 | 3171479 |
| the disposition of [2,3-14c]-methyl and [2,3-14c]-2-ethylhexyl acrylate in male wistar albino rats. | the disposition of methyl [2,3-14c]-acrylate (ma) and 2-ethylhexyl [2,3-14c]-acrylate (eha) following intraperitoneal and oral administration to rats has been studied. the 14c found in the tissues was mainly associated with liver, kidneys and lungs. loss of 14c from these tissues occurred fairly rapidly, excluding the rats given eha intraperitoneally. most of the administered acrylates underwent rapid metabolism and excretion with expired air (more than 50% of the dose and urine (10-50% of the d ... | 1988 | 3196152 |
| excision of cutaneous tumors in the horse using histologic guidance. | a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ... | 1988 | 3227634 |
| individual free fatty acids and lactate uptake in the human heart during severe sepsis. | coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ... | 1988 | 3232957 |
| vibrio alginolyticus cellulitis following coral injury. | infections associated with marine activities, particularly work or recreation in salt water, present unique diagnostic challenges for the infectious disease practitioner. those caused by halophilic, non-cholera vibrio species are increasingly being recognized in clinical practice. they typically follow saltwater injuries, especially those associated with coral. because these infections can be both severe and life-threatening, a consideration of halophilic vibrio species in the differential diagn ... | 1988 | 3242316 |
| biotechnological applications of lipid microstructures. based on the proceedings of the workshop on technological applications of phospholipid bilayers, vesicles, and thin films, january 5-9, 1986, tenerife, canary islands; and the workshop on biotechnological applications of membrane studies, october 18-22, 1987, san sebastian, spain. | | 1988 | 3250236 |
| dose-dependent effects of oral and intravenous glucose on insulin secretion and clearance in normal humans. | insulin secretion and clearance were studied in 2 groups of 7 normal subjects who each received 25, 50, and 100 g of glucose either orally or intravenously (iv) on separate occasions. insulin secretion rates were calculated during a 1-h base line and for 5 h after glucose administration from a two-compartmental analysis of peripheral c-peptide concentrations using individual kinetic parameters derived after iv bolus injections of biosynthetic human c-peptide. incremental glucose areas after oral ... | 1988 | 3279811 |
| reversibility of decreased insulin-stimulated glucose transport capacity in diabetic muscle with in vitro incubation. insulin is not required. | the mechanisms by which insulin deficiency affects muscle glucose transport were investigated. epitrochlearis muscles from rats with streptozotocin-induced diabetes and from controls were incubated in vitro for 0.5-14 h. the incubation was shown not to impair muscle energy stores or tissue oxygenation. diabetes decreased basal 3-o-methylglucose transport by 40% (p less than 0.01), and insulin-stimulated (20 milli-units/ml) glucose transport capacity by 70% (p less than 0.001). in vitro incubatio ... | 1987 | 3294836 |
| [man in prolonged space flight]. | | 1987 | 3307203 |
| [colibacillosis in calves, especially enterotoxic e. coli infections]. | developments in the knowledge of colibacillosis are reviewed in the series of 'papers in the past and present.' the starting-point of this paper is provided by the paper by h. veenstra:' a note on diseases of young breeding calves in the province of friesland' (1894) but particularly, by the study by j. poels: 'report on calf disease in the netherlands' which appeared in 1899. a historical review of bacteriological features in the pathogenesis of neonatal diarrhoea in calves and their significan ... | 1987 | 3310331 |
| photoperiodic modification of negative and positive feedback effects of oestradiol on lh secretion in ovariectomized goats. | ovariectomized shiba goats carrying an oestradiol implant (4-10 pg/ml) were kept under a short-day light regimen (10l:14d; group 1, n = 4) or a long-day regimen (16l:8d; group 2, n = 4). plasma lh concentrations were lower (p less than 0.05) in group 2 than in group 1 between days 40 and 200, suggesting an enhanced negative feedback effect of oestradiol on lh secretion under a long-day regimen. on days 30, 60, 100, 149 and 279, an lh surge was induced by i.v. infusion of oestradiol for 48 h; the ... | 1987 | 3656284 |
| energy cost and metabolic regulation during intermittent and continuous tetanic contractions in human skeletal muscle. | muscle atp turnover, glycogenolytic, and glycolytic rates were estimated to compare the energy cost and glycolytic regulation of 102.4 s of continuous and intermittent stimulation. quadriceps femoris muscles of male subjects were stimulated at 20 hz for one continuous contraction (n = 6) or a series of 64 contractions (1.6 s on, 1.6 s off; n = 6). leg blood flow was occluded and muscle biopsies were obtained at rest and following 51.2 and 102.4 s of contraction time in both conditions. isometric ... | 1988 | 3370544 |
| risk assessment for acquiring meningitis tuberculosis among children not vaccinated with bcg: a case-control study. | this case-control study was conducted to assess the risk, among children aged 0-12 years, of developing meningitis tuberculosis (mt) associated with a lack of intradermal bcg vaccination. cases (45) of mt admitted for treatment at the fundacao benjamin guimaraes hospital (belo horizonte, mg, brazil), from 1975 to 1981, were matched for age at hospitalization, date of hospitalization and nutritional status, with two types of controls--patients with acute diarrhoea (ad) and patients with acute non ... | 1988 | 3384537 |
| progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment. | sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ... | 1988 | 3386246 |
| use of mild detergent gel electrophoresis for isolation and characterization of the kidney brush border d-glucose transporter. | gradient gel electrophoresis was performed under mild detergent conditions to separate pig kidney brush border membrane proteins and to identify the smallest functional molecular protein entity of the d-glucose transporter. the various protein bands obtained from the nondenaturing gel system in a semipreparative scale were eluted by electrodialysis. these proteins were then reintegrated into proteoliposomes and tested for d-glucose-inhibitable [3h]phlorizin binding. the d-glucose transporter had ... | 1987 | 3425911 |
| effect of pre- and postnatal exposure to alcohol on perivenous and periportal neonatal rat hepatocytes. | hepatocytes from 12-day-old rats, pre and postnatally exposed to alcohol and pair-fed controls, were isolated and subfractionated in six cell subpopulations on percoll density gradients. these cells were characterized using biochemical, stereological and cytofluorometric methods. our results show that the low density cells (f2) are mainly perivenous cells, whereas the heavier cells (f6) were primarily periportal cells. these results were confirmed by alanine aminotransferase (alat), and glutamat ... | 1987 | 3426691 |
| evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio. | enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ... | 1986 | 3697846 |
| collagen binding in clinical isolates of staphylococcus aureus. | collagen binding was examined in 90 strains of staphylococcus aureus derived from patient samples. slightly under one-half (39 of 90) of the s. aureus strains bound collagen. collagen binding in s. aureus did not correlate with either immunoglobulin g or fibronectin binding by these organisms. chi-square analysis of isolates obtained from patients with complicated bacteremia (bacteremia associated with deep tissue infection) compared with isolates from patients with uncomplicated bacteremia (bac ... | 1987 | 3429618 |
| development and characterization of a rat histiocyte-macrophage tumor line. | a transplantable macrophage-like cell line has been obtained and established in w rats. this cell line is in its 85th passage and is perhaps the only established macrophage-like cell line that grows rapidly intraperitoneally in rats. the cells possess some of the typical characteristics of macrophages, such as adherence to glass, phagocytosis, presence of fc receptors and c3d receptors, la determinants, leukocyte common antigen, lysozyme, non-specific esterase, and glycogen. the tumor also grows ... | 1986 | 3457975 |
| effect of reperfusion on o2 supply/consumption balance in ischemic canine left ventricle. | this study was designed to assess the effects of reperfusion on regional o2 supply and o2 consumption of ischemic areas of the myocardium in 15 anesthetized open-chested dogs. the left anterior descending coronary artery (lad) was occluded for 6 h (n = 8), 2 h (n = 5), 2-h occlusion followed by 4-h period of reperfusion (n = 7), and 10-min occlusion followed by 90-min period of reperfusion (n = 3). small artery and vein o2 saturations obtained microspectrophotometrically were combined with regio ... | 1988 | 3147502 |
| renal apical membrane cholesterol and fluidity in regulation of phosphate transport. | renal proximal tubule cells adapt to dietary phosphate (pi) restriction by increasing pi transport independent of parathyroid hormone, vitamin d metabolites, or serum ca2+. to determine the underlying cellular mechanism(s), brush border (bbm) and basolateral membranes (blm) were isolated from growing male rats fed a synthetic diet containing variable levels of pi (0.1-1.4%). dietary pi restriction was without effect on either bbm or blm total lipid phosphorus, individual phospholipid species, or ... | 1985 | 3160247 |