| characterization of a catalase gene from aeromonas veronii, the digestive-tract symbiont of the medicinal leech. | the catalase gene kata of the medicinal leech symbiont aeromonas veronii bv. sobria was cloned, sequenced, and functionally characterized. southern hybridization, using an a. veronii kata-specific hybridization probe, suggested the presence of a single gene copy in many aeromonas species. a. veronii kata consisted of 1446 nt encoding a protein with a high degree of similarity to the small-subunit group iii bacterial catalases. a catalase-null mutant (jg186) was constructed through gene-replaceme ... | 2007 | 17526846 |
| spectroscopic studies on cobalt(ii)-substituted metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | in an effort to probe the structure of a group bb metallo-beta-lactamase, co(ii)-substituted imis was prepared and characterized by electronic absorption, nmr, and epr spectroscopies. imis containing 1 equiv of co(ii) (co(ii)(1)-imis) was shown to be catalytically active. electronic absorption studies of co(ii)(1)-imis revealed the presence of two distinct features: (1) an intense sulfur to co(ii) ligand to metal charge transfer band and (2) less intense, co(ii) ligand field transitions that sug ... | 2005 | 15794654 |
| [isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba]. | extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ... | 2007 | 17554439 |
| the role of c-di-gmp signaling in an aeromonas veronii biovar sobria strain. | aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. it is shown that all isolates of persistent aeromonas clones show strong biofilm formation ability. c-di-gmp regulates biofilm formation in many bacteria. to investigate the impact of c-di-gmp signaling, we introduced heterologous ggdef and eal domain proteins from salmonella typhimurium to an aeromonas veronii biovar sobria strain. overexpression of the ggdef domain protein adra increased c-di-gmp concentration ... | 2007 | 17573931 |
| complement resistance is essential for colonization of the digestive tract of hirudo medicinalis by aeromonas strains. | from the crop of the medicinal leech, hirudo medicinalis, only aeromonas veronii bv. sobria can be cultured consistently. serum-sensitive a. veronii mutants were unable to colonize h. medicinalis, indicating the importance of the mammalian complement system for this unusual simplicity. complementation of one selected mutant restored its ability to colonize. serum-sensitive mutants are the first mutant class with a colonization defect for this symbiosis. | 2003 | 12839811 |
| study of the effect of the midgut bacterial flora of culex quinquefasciatus on the susceptibility of mosquitoes to japanese encephalitis virus. | culex quinquefasciatus mosquitoes were collected from the field and microbial flora was isolated from their midgut. isolates belonging to 13 different genera were obtained. four most abundant isolates viz. pseudomonas sp., acinetobacterjunii, staphylococcus epidermis and aeromonas culicicola were used to study their effect on the susceptibility of mosquitoes to japanese encephalitis virus (jev). incorporation of individual isolates in the mosquito bloodmeal resulted in an increased susceptibilit ... | 2002 | 12693864 |
| molecular cloning, sequencing and characterization of omp48, the gene encoding for an antigenic outer membrane protein from aeromonas veronii. | to clone, sequence and characterize the gene encoding the omp48, a major outer membrane protein from aeromonas veronii. | 2003 | 12694457 |
| genetic linkage of the penicillinase gene, amp, and blrab, encoding the regulator of beta-lactamase expression in aeromonas spp. | aeromonas hydrophila t429125, a human clinical isolate, possesses three coordinately inducible beta-lactamases encoded by amph (class d beta-lactamase), ceph (class c beta-lactamase) and imih (class b beta-lactamase). we report that upstream of amph there are two genes, blra and blrb, encoding a putative two-component regulatory system. pcr studies revealed the same blrab-amp gene arrangement in all aeromonas spp. isolates tested; namely, aeromonas veronii bv. sobria, aeromonas jandaei, aeromona ... | 2003 | 12746371 |
| tracheobronchitis caused by aeromonas veronii biovar sobria after near-drowning. | a 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of aeromonas sp. in the water. an isolate of aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. the causality was supported by the massive growth of a. veronii in bronchial secretion, the presence of a type iii secretion system in the bacterial isolate, ... | 2007 | 17965361 |
| sequential binding of cobalt(ii) to metallo-beta-lactamase ccra. | in an effort to probe co(ii) binding to metallo-beta-lactamase ccra, epr, exafs, and (1)h nmr studies were conducted on ccra containing 1 equiv (1-co(ii)-ccra) and 2 equiv (co(ii)co(ii)-ccra) of co(ii). the epr spectra of 1-co(ii)-ccra and co(ii)co(ii)-ccra are distinct and indicate 5/6-coordinate co(ii) ions. the epr spectra also reveal the absence of significant spin-exchange coupling between the co(ii) ions in co(ii)co(ii)-ccra. exafs spectra of 1-co(ii)-ccra suggest 5/6-coordinate co(ii) wit ... | 2006 | 16430228 |
| evolutionary and diagnostic implications of intragenomic heterogeneity in the 16s rrna gene in aeromonas strains. | sequencing 16s rrna genes (ssu) cloned from aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. the ssu copies from aeromonas veronii lmg13695 clustered with sequences from four aeromonas species. these results demonstrate intragenomic heterogeneity of ssu and suggest caution when using ssu to identify aeromonads. | 2005 | 16159790 |
| aeromonas-acanthamoeba interaction and early shift to a viable but nonculturable state of aeromonas by acanthamoeba. | to investigate the hypothesis that amoeba may comprise a significant environmental reservoir for aeromonas, acanthamoeba-aeromonas interaction experiments were performed. | 2008 | 18194253 |
| severe diarrhoea caused by aeromonas veronii biovar sobria in a patient with metastasised gist. | this report describes the isolation of aeromonas veronii biovar sobria as the causative enteropathogen of diarrhoea in an oncological patient after failure of detection of other infectious agents. the case points out the severe and long course of the infection, the diagnostic dilemma, and the prompt recovery after antibiotic treatment. | 2007 | 18254499 |
| the major aeromonas veronii outer membrane protein: gene cloning and sequence analysis. | the gene encoding the major outer membrane protein (omp) from aeromonas veronii, omp38, was cloned and characterized. sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20-amino-acid signal peptide at the n-terminal and the consensus sequence ala-x-ala (ala-asn-ala) as the signal peptidase i recognition site. the mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 da. the degree of iden ... | 2005 | 16252131 |
| effect of packaging atmosphere on the microbial attributes of pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c. | effect of packaging atmosphere (air and under different modified atmospheres (mas), 40% co2/60% o2, 50%/50% o2, 60% co2/40% o2, 70% co2/30% o2 and 40% co2/30% o2/30% n2) on the microbial and biochemical attributes of fresh pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c was investigated. trimethylamine nitrogen (tma-n) and thiobarbituric acid (tba) values remained lower than the proposed acceptability limits throughout the storage period. results demonstrated that storage of pearls ... | 2008 | 18355677 |
| [identification of closely related bacteria via phylogenetic methods]. | to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences. | 2007 | 18067224 |
| plasmid-mediated qnrs2 determinant from a clinical aeromonas veronii isolate. | | 2008 | 18505860 |
| a distinct cause of necrotizing fasciitis: aeromonas veronii biovar sobria. | aeromonas veronii biovar sobria (veronii var. sobria) has not been implicated in the pathogenesis of necrotizing fasciitis in north america. | 2007 | 17999586 |
| culture-independent characterization of the digestive-tract microbiota of the medicinal leech reveals a tripartite symbiosis. | culture-based studies of the microbial community within the gut of the medicinal leech have typically been focused on various aeromonas species, which were believed to be the sole symbiont of the leech digestive tract. in this study, analysis of 16s rrna gene clone libraries confirmed the presence of aeromonas veronii and revealed a second symbiont, clone pw3, a novel member of the rikenellaceae, within the crop, a large compartment where ingested blood is stored prior to digestion. the diversit ... | 2006 | 16820471 |
| segmental ascending colitis associated with aeromonas veronii biovar sobria. | | 2006 | 16732806 |
| distribution of aeromonas species in the intestinal tracts of river fish. | aeromonas isolates were obtained from fish intestines, water, and sediments from an urban river and identified by the dna-dna microplate hybridization method. the isolates were aeromonas veronii (22%), aeromonas caviae (18%), aeromonas hydrophila (13%), aeromonas sobria (8%), aeromonas jandaei (7%), and other aeromonas spp. (33%). aeromonas species occurred at high densities with high incidences, regardless of season. the results strongly suggest that aeromonads are indigenous in fish intestines ... | 1995 | 16535171 |
| mechanistic studies on the mononuclear znii-containing metallo-beta-lactamase imis from aeromonas sobria. | in an effort to understand the reaction mechanism of a b2 metallo-beta-lactamase, steady-state and pre-steady-state kinetic and rapid freeze quench electron paramagnetic resonance (epr) studies were conducted on imis and its reaction with imipenem and meropenem. ph dependence studies revealed no inflection points in the ph range of 5.0-8.5, while proton inventories demonstrated at least 1 rate-limiting proton transfer. site-directed mutagenesis studies revealed that lys224 plays a catalytic role ... | 2006 | 16939225 |
| prevalence of environmental aeromonas in south east queensland, australia: a study of their interactions with human monolayer caco-2 cells. | to investigate the prevalence of aeromonas in a major waterway in south east queensland, australia, and their interactions with a gut epithelial model using caco-2 cells. | 2006 | 16968308 |
| phylogenetic identification of aeromonas strains isolated from carcasses of pig as new members of the species aeromonas allosaccharophila. | the first description of the species aeromonas allosaccharophila was only based on two strains (the type strain cect4199, and a duplicate cect4200) isolated from diseased elvers (anguilla anguilla) of an eel-farm located in valencia, spain, and one stool isolate (atcc35942) from a female with diarrhoea and food poisoning in south carolina, u.s.a. in the present study, 17 aeromonas isolates obtained from carcasses of pigs and from the equipment for the cleaning process, and one strain recently is ... | 2007 | 17080292 |
| x-ray absorption spectroscopy of the zinc-binding sites in the class b2 metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | x-ray absorption spectroscopy was used to investigate the metal-binding sites of imis from aeromonas veronii bv. sobria in catalytically active (1-zn), product-inhibited (1-zn plus imipenem), and inactive (2-zn) forms. the first equivalent of zinc(ii) was found to bind to the consensus zn(2) site. the reaction of 1-zn imis with imipenem leads to a product-bound species, coordinated to zn via a carboxylate group. the inhibitory binding site of imis was examined by a comparison of wild-type imis w ... | 2006 | 17087519 |
| [postoperative infection of an epigastric hematoma caused by aeromonas veronii biovar sobria]. | a 52-year-old man with a gastric signet-ring cell carcinoma but without acute symptoms was admitted for reconstructive surgery of the gastrointestinal tract. | 2006 | 17109273 |
| cloning of atp-dependent protease clpxp genes in aeromonas veronii. | | 2006 | 17169116 |
| culturable and vbnc vibrio cholerae: interactions with chironomid egg masses and their bacterial population. | vibrio cholerae, the etiologic agent of cholera, is autochthonous to various aquatic environments. recently, it was found that chironomid (nonbiting midges) egg masses serve as a reservoir for the cholera bacterium and that flying chironomid adults are possible windborne carriers of v. cholerae non-o1 non-o139. chironomids are the most widely distributed insect in freshwater. females deposit egg masses at the water's edge, and each egg mass contains eggs embedded in a gelatinous matrix. hemagglu ... | 2007 | 17186156 |
| detection of conjugation related type four secretion machinery in aeromonas culicicola. | background: aeromonas sp. can now be considered relatively common enteropathogens due to the increase of diseases in humans. aeromonas culicicola is a gram negative rod-shaped bacterium isolated for the first time from the mosquito mid-gut, but subsequently detected in other insects and waters also. our previous study discovered that a. culicicola harbors three plasmids, which we designated as pac3249a, pac3249b and pac3249c. we investigated and report here the existence and genetic organization ... | 2006 | 17205119 |
| [rapidly progressive myonecrosis by aeromonas veronii biotype sobria]. | myonecrosis due to aeromonas spp is exceptional. we report the case of a diabetic patient with liver cirrhosis who developed a rapidly progressive myonecrosis by aeromonas veronii biotype sobria. the portal of entry was an injury after falling down in an irrigation canal. the outcome was not favourable and surgical amputation of left leg was performed in spite of antibiotic treatment with cefotaxime and tobramicin. aeromonas spp can be very aggressive and this microorganism should be considered ... | 2006 | 17222071 |
| aeromonas hemolytic uremic syndrome. a case and a review of the literature. | although rarely, hemolytic uremic syndrome can be induced by aeromonas. we report a case in a 40-year-old spanish female produced by aeromonas veronii bv. sobria and review the previous cases described in the literature. this is the 2nd case described in adults. | 2007 | 17300902 |
| biochemical characterization of three putative atpases from a new type iv secretion system of aeromonas veronii plasmid pac3249a. | type four secretion systems (tfss) are bacterial macromolecular transport systems responsible for transfer of various substrates such as proteins, dna or protein-dna complexes. tfsss encode two or three atpases generating energy for the secretion process. these enzymes exhibit highest sequence conservation among type four secretion components. | 2010 | 20144229 |
| importance of flagella and enterotoxins for aeromonas virulence in a mouse model. | a genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaa/flab, flag), lateral flagella (lafa), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. when 16 aeromonas hydrophila strains, seven aeromonas veronii strains, and seven aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal inj ... | 2007 | 17496975 |
| new gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts. | many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant amazonian leech, haementeria ghilianii, and two others from placobdelloides species. these hosts each possess a different mycetome or esophageal organ morphology wher ... | 2005 | 16151107 |
| molecular mechanisms of quinolone resistance in clinical isolates of aeromonas caviae and aeromonas veronii bv. sobria. | mutations in quinolone targets were studied together with quinolone efflux pump activation and plasmid-mediated quinolone resistance determinants in nalidixic-acid-resistant isolates of aeromonas caviae and aeromonas veronii. among 135 clinical aeromonas spp. isolated from stools of patients with gastrointestinal symptoms, 40 nalidixic acid-resistant strains belonging to a. caviae and a. veronii were selected and their susceptibility to different quinolones (ciprofloxacin, norfloxacin, ofloxacin ... | 2010 | 20890847 |
| molecular requirements for the colonization of hirudo medicinalis by aeromonas veronii. | | 2006 | 16623399 |
| interaction between innate immune cells and a bacterial type iii secretion system in mutualistic and pathogenic associations. | animals house a community of bacterial symbionts in their digestive tracts that contribute to their well being. the medicinal leech, hirudo verbana, has a remarkably simple gut population carrying two extracellular microbes in the crop where the ingested blood is stored. this simplicity renders it attractive for studying colonization factors. aeromonas veronii, one of the leech symbionts, can be genetically manipulated and is a pathogen of mammals. screening transposon mutants of a. veronii for ... | 2007 | 17517651 |
| use of the novel phylogenetic marker dnaj and dna-dna hybridization to clarify interrelationships within the genus aeromonas. | the interrelationships of 27 aeromonas strains were investigated using dnaj sequences and dna-dna hybridization. dnaj sequence similarities showed a stronger relationship with dna-dna relatedness values than did 16s rrna gene sequence similarities. additionally, dnaj sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16s rrna gene sequences for the differentiation of strains at the species level. relationships among aeromonas species were theref ... | 2007 | 17551035 |
| aeromonas veronii, a tributyltin (tbt)-degrading bacterium isolated from an estuarine environment, ria de aveiro in portugal. | organotin compounds are used in a variety of industrial processes therefore their subsequent discharge into the environment is widespread. bacteria play an important role in biogeochemical transformations acting as natural decontamination agents. therefore, screening for tributyltin (tbt)-resistant and -degrading bacteria is relevant for the selection of isolates with decontamination ability of these polluted areas. with this purpose, 50 strains were isolated from sediment and water from ria de ... | 2007 | 17719084 |
| detection of aaca-aphd, qaced1, mara, flor, and teta genes from multidrug-resistant bacteria: comparative analysis of real-time multiplex pcr assays using evagreen(®) and sybr(®) green i dyes. | we have developed multiplex real-time pcr assays that utilize dna-intercalating dyes, sybr green i (sg) and evagreen (eg), with two primer sets (set 1=qaced1, teta and aaca-aphd; set 2=teta, mara, and flor) to simultaneously amplify the qaced1, teta, aaca-aphd, mara, and flor genes. validity of the multiplex pcr assays was confirmed by testing 83 bacterial isolates, including staphylococcus aureus (28 isolates), enterococcus spp. (17 isolates), salmonella enterica serovar typhimurium (8 isolates ... | 2011 | 21256956 |
| aeromonas spp. human isolates induce apoptosis of murine macrophages. | interactions of aeromonas caviae, aeromonas veronii biotype sobria, and aeromonas hydrophila strains isolated from fecal specimens of humans with gastroenteritis on murine macrophages, j774 cells, were investigated. analyses of cellular morphology and dna fragmentation in phagocytes infected with these strains exhibited typical characteristic features of cells undergoing apoptosis. we observed the morphological changes, including condensation of nuclear chromatin, formation of apoptotic bodies a ... | 2009 | 19005723 |
| characterization of vibrio cholerae isolated from the aquatic basins of the state of pernambuco, brazil. | through a continuous bacteriological monitoring programme carried out by the health secretariat of the state of pernambuco, brazil, two isolates of vibrio cholerae o1 el tor ogawa were discovered in an endemic area in 2001, during a cholera inactive period, along with six v. cholerae non-o1/non-o139 strains and two aeromonas veronii biovar sobria strains showing an unusual characteristic of agglutination with o1 antiserum. between that time and 2005, eight other o1 isolates were found. the virul ... | 2008 | 18258274 |
| pathogenic potential of a collagenase gene from aeromonas veronii. | the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ... | 2008 | 18388966 |
| conformational changes in the metallo-beta-lactamase imis during the catalytic reaction: an epr spectrokinetic study of co(ii)-spin label interactions. | metallo-beta-lactamases are responsible for conferring antibiotic resistance on certain pathogenic bacteria. in consequence, the search for inhibitors that may be useful in combating antibiotic resistance has fueled much study of the active sites of these enzymes. there exists circumstantial evidence that the binding of substrates and inhibitors to metallo-beta-lactamases may involve binding to the organic part of the molecule, in addition to or prior to binding to one or more active site metal ... | 2008 | 18528987 |
| [characterization of aeromonas spp isolates from newborns hospitalized]. | aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ... | 2008 | 18545840 |
| purification and characterization of cytolytic toxins produced by aeromonas hydrophila and aeromonas veronii biotype sobria strains. | cytolytic toxins produced by aeromonas hydrophila and aeromonas veronii biotype sobria strains were partially purified from culture filtrates by two steps of purification: ammonium sulfate precipitation and hydrophobic chromatography using phenyl-sepharose cl-4b. hemolytic activity was detected in one or two peaks in elution profile. purified toxins were also cytotoxic to vero and cho cells. moreover, these toxins revealed cytotonic activity to cho cells. | 2006 | 16878602 |
| complete genome sequence of aeromonas veronii strain b565. | aeromonas veronii strain b565 was isolated from aquaculture pond sediment in china. we present here the complete genome sequence of b565 and compare it with 2 published genome sequences of pathogenic strains in aeromonas genus. the result represents an independent step-wise acquisition of virulence factors of pathogenic strains in this genus. | 2011 | 21551296 |
| aeromonas culicicola pidiyar et al. 2002 is a later subjective synonym of aeromonas veronii hickman-brenner et al. 1987. | we re-investigated the taxonomic affiliation between aeromonas culicicola pidiyar et al. 2002 and aeromonas veronii hickman brenner et al. 1987, two species that have previously been shown to be closely related on the basis of gyrb and rpod gene sequencing and amplified fragment length polymorphism fingerprinting. from extended biochemical characterization and fatty acid analysis, it was concluded that a. culicicola did not constitute a unique phenotypic group in the genus aeromonas but instead ... | 2005 | 16156118 |
| interaction of aeromonas spp. human isolates with murine macrophages. | this study aimed to evaluate phagocytic and bactericidal activity of macrophages originated from murine j774 macrophage cell line against 26 strains of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila isolated from human fecal specimens. the analysis of phagocytic index (pi) indicated these strains were phagocytosed in low numbers, below 1.1 x l0(-3) for 65% strains of a. veronii biotype sobria and a. hydrophila. the highest pi value (0.019) was noted in murine macroph ... | 2008 | 19123303 |
| development of imipenem resistance in an aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis. | several imipenem-susceptible and -resistant aeromonas veronii biovar sobria isolates with different morphologies and antimicrobial susceptibilities recovered from bile samples of a patient with cholangitis were analysed. these isolates belonged to the same clone and the imipenem-resistant strains showed overexpression of the imis gene, encoding a chromosomal carbapenemase. these results should make clinicians aware of the possible emergence of multidrug-resistant a. veronii biovar sobria, perhap ... | 2009 | 19273640 |
| cloning and expression of an outer membrane protein ompw of aeromonas hydrophila and study of its distribution in aeromonas spp. | the main aims of this study were to clone and express an outer membrane protein (omp), ompw, of aeromonas hydrophila and to study its distribution in aeromonas spp. | 2009 | 19426281 |
| new structural variants of homoserine lactones in bacteria. | n-acylhomoserine lactones (ahls) are used by a wide variety of bacteria for cell-cell communication in "quorum-sensing". these compounds are derived from l-homoserine lactone and a fatty acid, which varies in chain-length, degree of saturation, and the presence or absence of an oxygen atom at c-3. in this study we describe for the first time the occurrence of acyl chains carrying a methyl branch, and present a gc-ms-based method that can be used to distinguish these compounds from unbranched iso ... | 2009 | 19533714 |
| lactobacillus plantarum (vr1) isolated from an ayurvedic medicine (kutajarista) ameliorates in vitro cellular damage caused by aeromonas veronii. | abstract: | 2011 | 21707995 |
| differential immune responses of the green neon shrimp (neocaridina denticulate) to dipropyl phthalate. | this study set out to understand the sublethal effect of xenobiotic phthalate esters (paes) on the relationship between the susceptibility of shrimp to bacterial infection and the immune response of the shrimp. neocaridina denticulate were exposed to different concentrations of the pae dipropyl phthalate (dprp), and mortality and six immune parameters were measured on days 1, 3, 5, and 10 after exposure. on days 1 and 3 after exposure, shrimp exposed to 0, 1, 5, 10, and 50 mg/l of dprp and chall ... | 2011 | 21740974 |
| prevalence of genes encoding the type three secretion system and the effectors aext and aexu in the aeromonas veronii group. | the prevalence of important virulence factors, the type iii secretion system (t3ss) and two t3ss-dependent toxins, aext and aexu, was evaluated in the aeromonas veronii group (avg). members of the avg have a broad host range, including vertebrates and invertebrates, and form a variety of associations, spanning from pathogenic to mutualistic. our avg strain collection consists of human, duck, eel, and leech isolates. these isolates were examined for the presence of the t3ss, aext, and aexu throug ... | 2009 | 19534604 |
| Diversity and antibiotic resistance of Aeromonas spp. in drinking and waste water treatment plants. | The taxonomic diversity and antibiotic resistance phenotypes of aeromonads were examined in samples from drinking and waste water treatment plants (surface, ground and disinfected water in a drinking water treatment plant, and raw and treated waste water) and tap water. Bacteria identification and intra-species variation were determined based on the analysis of the 16S rRNA, gyrB and cpn60 gene sequences. Resistance phenotypes were determined using the disc diffusion method. Aeromonas veronii pr ... | 2011 | 21907383 |
| aeromonas fluvialis sp. nov., isolated from a spanish river. | a gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(t), was isolated from a water sample collected from the muga river, girona, north-east spain. preliminary analysis of the 16s rrna gene sequence showed that this strain belonged to the genus aeromonas, the nearest species being aeromonas veronii (99.5 % similarity, with seven different nucleotides). a polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrb, rpod, reca, dnaj and g ... | 2010 | 19648345 |
| ompa is an adhesion factor of aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract. | in the present study, we focused on one of the aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion. | 2008 | 18828791 |
| mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels. | mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ... | 2010 | 19900538 |
| cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram. | sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ... | 2010 | 3116451 |
| [dental findings in children with hearing disorders]. | tooth findings are reported of 972 children aged between 3 to 18 years who suffer from impaired hearing. logopedic pupils had significantly more tooth anomalies than those with impaired and normal hearing. the deaf pupils had significantly less dysgnathias than others with impaired hearing. as compared to those with normal hearing there was no significant difference. | 2002 | 124119 |
| [severe pneumonia caused by aeromonas veronii biovar sobria: a case report and review of the literature]. | to describe the clinical features of severe pneumonitis with aeromonas veronii biovar sobria. | 2008 | 19080598 |
| physician compliance in the management of hypertensive patients. | the aim of the present study was to investigate prospectively the value of different forms of intervention in the quality of diagnosis and treatment of essential hypertension in an outpatient clinic. in a first phase (phase 0) 115 patients with a blood pressure of greater than or equal to 140/greater than or equal to 90 mmhg at the initial visit in our outpatient clinic were registered. after a 3 months period the charts of these patients were reviewed. this review revealed that 61 (53%) of thes ... | 2012 | 3870473 |
| [the postcholecystectomy syndrome]. | | 2012 | 3454415 |
| ronald michael charles welldon. | | 2014 | 4097960 |
| [impressions of a study trip to japan]. | | 2015 | 4495220 |
| mechanism of action of beta-blockers in hypertension. | | 2001 | 14643 |
| pelvic displacement of a cardiac pacemaker following trauma. a case report. | the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope. | 2003 | 152472 |
| male transsexualism in england: sixteen cases with surgical intervention. | the syndrome of transsexualism is now regarded as a dinstinct entity separate from transvestism and homosexuality (benjamin, 1966; green and money, 1969). it refers to those patients who since childhood have been convinced of their wrong sex assignment and who wish to attain the sexual characteristics of the opposite sex and to live as a member of that sex. the present report is an initial one referring to the first group of transsexual patients studied at a postgraduate teaching hospital by a m ... | 2005 | 1169047 |
| changes in drug treatment after discharge from hospital in geriatric patients. | to ascertain changes in drug treatment of elderly patients after discharge from hospital and to identify areas of communication which may require improvement. | 2006 | 1393119 |
| using computers to take patient histories. | | 2011 | 3147730 |
| detection and characterization of virulence genes and integrons in aeromonas veronii isolated from catfish. | the presence of virulence genes and integrons was determined in 81 strains of aeromonas veronii isolated from farm-raised catfish. polymerase chain reaction (pcr) protocols were used to determine the presence of genes for cytotoxic enterotoxin (act), aerolysin (aera), two cytotonic enterotoxins (ast, alt), lipase (lip), glycerophospholipid:cholesterol acyltransferase (gcat), serine protease (ser), dnases (exu), elastase (ahyb) and the structural gene flagellin (fla) in the template dna. oligonuc ... | 2010 | 20227596 |
| atlanta's homeless find health care. | | 2013 | 3636083 |
| [colonoscopic follow up of rectocolonic cancer surgeries. assessment of 64 examinations]. | a collation of 64 colonoscopic examinations performed on 41 patients operated on for cancer of the colon or rectum is reported. eleven patients (26.8 percent) had positive follow-up endoscopic findings. in 13 enumerated tumoral proliferations. 8 benign polyps and 5 malignant growths were found, one of the latter on the anastomosis, and the other 4 on the remaining colon. 80 percent of these tumoral growths and all malignant tumors were discovered in the two years following surgery. when colonic ... | 2014 | 4004135 |
| an intrarenal foreign body. | | 2016 | 5041547 |
| [development of home care in vorarlberg]. | | 2012 | 3353111 |
| three permutations of percutaneous endoscopic gastrostomy. | | 2013 | 3710099 |
| eczema vaccinatum. | | 2014 | 4172112 |
| are steroids essential for successful maintenance of immunosuppression in heart transplantation? | to determine the necessity for maintenance dosages of prednisone in the management of heart transplant patients, a retrospective study was made of 86 patients undergoing orthotopic heart transplantation and two patients having heart-lung transplantation from june 1985 through october 1986. group 1 (n = 52) had maintenance immunosuppressive therapy that included cyclosporine, azathioprine, and prednisone. group 2 (n = 36) received only cyclosporine and azathioprine with no maintenance dosage of s ... | 2012 | 3316555 |
| perforations of the tympanic membrane following the use of goode-type 'long term' tympanostomy tubes. | a retrospective review of 64 goode 't' tube insertions is presented. these were inserted into 61 ears of 48 patients over a period of four years. at the time of review of these 61 ears, 34 tubes were found to be in situ, 27 had extruded spontaneously and three had been removed surgically. ten ears in which the tube had been extruded or removed had central perforations of the tympanic membrane of which eight had been present for more than six months. the number of previous myringotomies or gromme ... | 2011 | 3209952 |
| the antihypertensive effect of angiotensin-converting enzyme inhibition: a multifaceted mechanism of action. | | 2010 | 3030977 |
| economic rights of the institutionalized mentally disabled. | | 2011 | 3319919 |
| monitoring of long-term motor activity in depressed patients. | wrist motor activity was continuously recorded by a solid-state monitor in 12 patients with major depression throughout their stay in hospital; their clinical state was evaluated using three scales. during the day, activity troughs and immobility peaks occurred before noon and around 1500 hrs. activity level progressively increased, while the duration of immobility decreased, with clinical improvement. the immobility measurement appeared to be a valuable index of depression and is more sensitive ... | 2013 | 3779292 |
| [pharmacotherapy and renal function in the aged]. | | 2015 | 4585245 |
| [anatomical contributions of schaffhausen medical school of the 17th century]. | | 2015 | 4569048 |
| lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
| lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
| [experience with ultraviolet irradiation of the blood in chronic diseases]. | | 2016 | 4837149 |
| convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory. | although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ... | 2007 | 17596358 |
| the chemical use, abuse, and dependence scale (cuad). rationale, reliability, and validity. | this article describes the rationale for the development of the chemical use, abuse, and dependence scale (cuad). the instrument is in a semistructured interview format; it derives both substance use severity scores and dsm-iii-r substance use disorder diagnoses and can be administered in a short period with minimal training. the reliability and validity of the cuad are reported and appear satisfactory. the cuad is recommended for use as a detection, diagnostic, and treatment selection index in ... | 2005 | 1317464 |
| [photic maculopathy by low energy laser beam. a case report]. | the authors report a case of photic maculopathy that developed in a 30-year-old laboratory worker after repeated, consecutive exposure to low energy he-ne laser. pigmented foveal change and a small central scotoma in the right eye with window defect on fluorescein angiography characterized this maculopathy. photochemical damage is considered to be the cause of the maculopathy. | 2006 | 1797372 |
| [photic maculopathy by low energy laser beam. a case report]. | the authors report a case of photic maculopathy that developed in a 30-year-old laboratory worker after repeated, consecutive exposure to low energy he-ne laser. pigmented foveal change and a small central scotoma in the right eye with window defect on fluorescein angiography characterized this maculopathy. photochemical damage is considered to be the cause of the maculopathy. | 2006 | 1797372 |
| mouth opening after release of maxillomandibular fixation in fracture patients. | three hundred eleven patients with mandibular fractures were studied prospectively to ascertain the influence of jaw immobilization on mouth opening. maxillomandibular fixation caused a significant reduction in mouth opening whereas open reduction did not. furthermore, there appeared to be a relationship between the duration of immobilization and the degree of reduction of mouth opening such that the shorter the duration of fixation, the less the reduction. | 2012 | 3471923 |
| identification of aeromonas veronii genes required for colonization of the medicinal leech, hirudo verbana. | most digestive tracts contain a complex consortium of beneficial microorganisms, making it challenging to tease apart the molecular interactions between symbiont and host. the digestive tract of hirudo verbana, the medicinal leech, is an ideal model system because it harbors a simple microbial community in the crop, comprising the genetically amenable aeromonas veronii and a rikenella-like bacterium. signature-tagged mutagenesis (stm) was used to identify genes required for digestive tract colon ... | 2007 | 17616592 |
| virulence potential and genetic diversity of aeromonas caviae, aeromonas veronii, and aeromonas hydrophila clinical isolates from mexico and spain: a comparative study. | a comparative study of 109 aeromonas clinical isolates belonging to the 3 species most frequently isolated from patients with diarrhea in mexico and spain was performed to investigate the distribution of 3 prominent toxin genes and the gene encoding flagellin of lateral flagella; 4 well-established virulence factors in the genus aeromonas. the aerolysin-hemolysin toxin genes were the most prevalent, being present in 89% of the total isolates. the ast toxin gene was conspicuously absent from the ... | 2007 | 17898843 |
| [isolation and identification of pathogenic aeromonas veronii isolated from infected siberian sturgeon (acipenser baerii)]. | our study aimed at searching for the pathogenic factor causing siberian sturgeon (acipenser baerii) disease. | 2009 | 20069873 |
| plasmid-mediated qnrs2 determinant in an aeromonas caviae isolate recovered from a patient with diarrhoea. | a qnrs2 gene was identified in an aeromonas caviae isolate (mics of ciprofloxacin, norfloxacin and ofloxacin >32 mg/l) from a stool sample collected from a patient with gastroenteritis. the analysis of the gyra and parc genes revealed amino acid substitutions ser83-ile and ser80-thr, respectively. in addition, five out of 41 nalidixic acid-resistant aeromonas isolates studied (26 identified as aeromonas veronii bv sobria and 15 identified as a. caviae) showed ciprofloxacin resistance. the identi ... | 2010 | 19863591 |
| dental specialties--past, present and future. | | 2015 | 4444815 |
| resonance energy transfer in pepsin conjugates. | | 2016 | 4897805 |
| [use of optics in the examination and operations for diseases of the pharynx and larynx]. | | 2016 | 4767970 |