| generation of h-2 restricted cytotoxic t cells by trinitrophenylated proteins in vitro: specificity and requirements. | | 1979 | 90099 |
| m467: a murine iga myeloma protein that binds a bacterial protein. i. recognition of common antigenic determinants on salmonella flagellins. | we have studied the binding of m467, an iga murine myeloma protein, to flagellin from seven species of salmonella. it was found that m467 was reacting with antigenic determinants that were common to all the flagellins studied. these determinants were not related to serotypic antigens. electronmicrographs of unreduced m467 showed a variety of polymeric species bound to flagella in a manner that could produce immobilization as well as agglutination and precipitation through cross-linking of antige ... | 1979 | 90100 |
| specific absorption of t lymphocytes committed to soluble protein antigens by incubation on antigen-pulsed macrophage monolayers. | monolayers of macrophages (mphi) pulsed with antigen were used as immunosorbents for t lymphocytes from guinea pigs primed to soluble protein antigens. t lymphocytes were cultured on the mphi monolayers for 4 hr, then aspirated and reincubated on a fresh monolayer pulsed with the same antigen for a second and a third step. t lymphocytes so treated were selectively deprived of cells responding in assay for antigen-dependent proliferation against the antigen used for pulsing the absorbing monolaye ... | 1979 | 90101 |
| characterization of in vitro proliferative responses of human lymphocytes to leishmanial antigens. | leishmaniasis is an intracellular protozoal infection for which host defense is believed to depend on cellular immune mechanisms. the in vitro proliferative responses of lymphocytes from patients with leishmaniasis and from control subjects to leishmanial antigens were examined. only lymphocytes from patients responded to 1 microgram of leishmanial antigen/ml, whereas both patient and control lymphocytes responded to 10 micrograms/ml. the nonspecific responses were most likely not due to a mitog ... | 1979 | 90105 |
| 4th international workshop on human gene mapping. report of the committee on the genetic constitution of the x and y chromosomes. | | 1978 | 88299 |
| differential growth inhibition in two human carcinoma cell lines by cyclic adenosine 5'-monophosphate analogs. | the inhibition of cell replication in two human carcinoma cell lines by various cyclic amp analogs was explored. in all instances, the addition of the cyclic nucleotide phosphodiesterase inhibitor 1-methyl-3-isoburylxanthine resulted in synergistic growth inhibition by the analogs. a correlation was found between an analog's ability to inhibit growth and its ability to activate protein kinase. a differential effect of the breakdown product 8-bromo-amp (8-bramp) on cell replication in the two cel ... | 1979 | 90151 |
| counterimmunoelectrophoresis for rapid identification of blood-culture isolates. | a total of 303 blood cultures that were positive by examination of gram-stained smears were tested immediately by counterimmunoelectrophoresis for detection of bacterial antigens. antigen was detected in all 82 blood cultures containing streptococcus pneumoniae and 11 of 22 with klebsiella pneumoniae, two of two with haemophilus influenzae, and one of one with neisseria meningiditis. false-positive cross-reactions in 265 tests occurred only with pneumococcal omniserum in two cases of nongroupabl ... | 1979 | 86294 |
| [micromethod of carrying out the passive hemagglutination reaction for detecting antibodies to influenza a2 virus in tears and aqueous humor]. | | 1979 | 90190 |
| enigma of disodium cromoglycate action on mast cells. | in rat peritoneal mast cells, disodium cromoglycate showed no inhibitory effect on histamine release values if the cells were preincubated with the drug for 40 min prior to stimulation with antigen, compound 48/80 or atp. if the drug was added simultaneously with antigen or a low dose of compound 48/80, a repression of histamine release occurred. no such effect was noted if atp was employed as histamine liberator. | 1979 | 88418 |
| cyclic amp and lipogenesis in fat cells from thyroidectomized rats. | thyroid hormones regulate lipid metabolism by affecting lipogenesis as well as lipolysis. the present paper discusses the way thyroidectomy induced an enhancement in lipogenesis in rat fat cells. the doubling in the conversion of glucose to co2 and fatty acids seen after thyroidectomy was found to be due to a modification in the actual pathway of glucose metabolism: there was a preferential stimulation of the conversion of glucose to co2 by the pentose cycle (utilisation of [1-14c]glucose) while ... | 1979 | 89052 |
| [species and interspecies antigenic specificity of house dust mites (genus dermatophagoides)]. | | 1979 | 90327 |
| central transport and distribution of labelled glutamic and aspartic acids to the cochlear nucleus in cats: an autoradiographic study. | | 1979 | 90345 |
| detection by antihapten antibodies of liver-bound compounds related to azocarcinogens or their metabolites. | the localization of known azocarcinogens and metabolites such as p-aminoazobenzene and n-methyl-p-aminoazobenzene bound to components of liver cells of rats fed single or multiple doses of 3'-methyl-p-dimethylaminoazobenzene has been determined with the use of antibodies raised against p'-azo-p-aminoazobenzene and p'-azo-n-monomethyl-p-aminoazobenzene in the indirect fluorescent antibody procedure. these 2 antisera reacted with liver cells of rats fed 3'-methyl-p-dimethylaminoazobenzene, p'-amin ... | 1979 | 90352 |
| [pharmacological properties and antitumor activity of a preparation diiodo-benzo-tepa in experiments]. | experimental data on the pharmacokinetics, toxicity, antitumor activity of the antitumor drug diiodo-benzo-tepa are reported. | 1979 | 90429 |
| cross-neutralization tests on sheep pox, goat pox and contagious pustular dermatitis viruses. | in cross-neutralization tests in cell cultures, sheep pox and contagious pustular dermatitis (cpd) hyperimmune sera did not cross-react in the heterologous system. but goat pox hyperimmune serum neutralized both sheep pox and cpd viruses revealing a one-way serological relationship of goat pox virus with both sheep pox and cpd viruses. | 1979 | 90458 |
| immunological unresponsiveness in mice. ii. cellular basis of immunological unresponsiveness induced in foetal and neonatal mice by transfer of human gamma-globulin by the maternal route. | the cellular basis of the mechanism of immunological tolerance to human gamma-globulin (h gamma g) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single injection of tolerogen (deaggregated h gamma g) into the mothers was investigated using a cell transfer system and assays of passive haemagglutinating antibodies and plaque-forming cells to h gamma g. the results demonstrated that b cells are mainly involved in the tolerance induced on the fourteenth d ... | 1979 | 89080 |
| relations between fatty acids and oestrogen binding properties of pure rat alpha 1-foetoprotein. | a delipidation procedure based on treatment with charcoal at ph 3 has been applied to highly purified rat alpha 1-foetoprotein preparations. the oestrogen binding properties of the delipidated proteins have been studied with an equilibrium dialysis technique, and compared with the properties of the untreated foetal protein, as well as those of preparations reconstituted from the defatted alpha 1-foetoprotein and the removed lipids. an important increase has been evidenced for the binding levels ... | 1979 | 90528 |
| cells of origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase. | following injections of horseradish peroxidase into the cerebellum, the distribution of labeled neurons was studied in the whole length of the spinal cord of the rat. to find the ascending side of the axons, injections were made following hemisections at c1 or between c1 and c2. labeled spinocerebellar tract neurons were classified into two groups according to the axonal course in the spinal cord; one is composed of neurons with uncrossed ascending axons and the other, neurons with crossed ascen ... | 1979 | 90539 |
| in vitro studies of the replication of moloney murine leukemia virus. | | 1979 | 90575 |
| biological role of gramicidin s in spore functions. studies on gramicidin-s-negative mutants of bacillus brevis atcc9999. | gramicidin-s-negative mutants of bacillus brevis atcc9999 have been isolated with a remarkly higher yield after ethidium bromide or acridine orange treatment, than after n-methyl-n'-nitro-n-nitrosoguanidine treatment. four (miv, smr170, r5 and eb 16) of 38 isolated mutants were characterized with respect to the lesion in gramicidin-s-synthesizing activity. the mutants sporulate to the same extent as the parental strain except mutant smr 170 which sporulates less. however, mutant spores were more ... | 1979 | 90616 |
| relative affinity of antisera for myelin basic protein (mbp) and degree of affinity heterogeneity. | | 1979 | 90641 |
| the location and expression of idiotypic determinants in the immunoglobulin variable region--i. characterization of antibodies directed against the variable region of mouse myeloma immunoglobulins 315 and 460. | | 1979 | 90644 |
| [animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock]. | after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed. | 1979 | 90651 |
| characterization of the antigenic determinants of cholera toxin subunits. | the antigenic specificity of purified preparations of a subunit, b subunit, alpha chain, and gamma chain of cholera toxin was studied by double immunodiffusion and radioimmunoassay with antisera produced in rabbits and mice. rabbits immunized with a subunit produced serum antibodies which were capable of binding radiolabeled a subunit, alpha chain, and b subunit. rabbits immunized with alpha chain produced serum antibodies that would bind radiolabeled alpha chain and a subunit. rabbits immunized ... | 1979 | 90654 |
| the indirect rat mast cell degranulation test reconsidered. | the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ... | 1979 | 90655 |
| complement profiles in monkeys subjected to aggregate (immune complex) anaphylaxis, and following injection of soluble and particulate polysaccharides. | complement profiles were established in four groups of macaca irus monkeys: (i) aggregate (immune complex) anaphylaxis was induced following immunization, with ovalbumin. upon challenge, systemic arterial pressure decreased from 115 to 50 mm hg (mean values) in 10 min. the complement profiles revealed decreases in: c1q to less than 10% of initial value within 5 min; c4 proportional to hypotension; c3 slowly to 60% at 24 h; c5, c6, c7, c8 and factor b to about 80% of initial value in 5--30 min. c ... | 1979 | 90656 |
| antigens in penicillin allergy. iv. induction of ige antibody response in mice after daily treatment with contaminated but not with pure penicillin. | | 1979 | 90657 |
| establishment, characterization and virus expression of cell lines derived from radiation- and virus-induced lymphomas of c57bl/ka mice. | permanent cell lines have been established in vitro from lymphoid tumors induced in c57bh/ka mice by fractionated x-irradiation or by inoculation of the radiation leukemia virus (radov). the cultured cells are lymphoblastic, replicate rapidly in vitro, and are tumorigenic in vivo. the cell surface markers thy 1, ly 1, ly 2,3 and gix are expressed by the lymphoid tumor cells in the mouse and persist in the corresponding cell lines; expression of the h-2 and tl antigens is greatly reduced during i ... | 1979 | 90660 |
| tobacco leaf mesophyll peroxidase. isolation, purification and some properties. | two isoperoxidases, p1 and p2, were isolated from tobacco mesophyll leaves by the combined methods of ammonium sulphate precipitation, page and deae-cellulose column chromatography. the ph otpima for p1 and p2 were 7.0 and 7.5 respectively. the values of km for h2o2 were 1.82 and 0.87 mm for p1 and p2. results from this investigationsuggest that these isoperoxidases differ in their electrophoretic, catalytic and physical properties. | 1979 | 90669 |
| autoregulation of germ tube formation by candida albicans. | germ tube formation by candida albicans is at least partially controlled by a product(s) of the yeast phase of the organism which is released from cells upon incubation at 37 degrees c in tissue culture medium or fetal calf serum. this germination regulatory substance is stable under conditions of lyophilization and heating of 70 degrees c, but becomes inactivated at ph values of 4.0 and 9.5. a germination regulatory substance was produced by both strains of c. albicans tested and by a strain of ... | 1979 | 89087 |
| on the kinetics and maximal capacity of the system for rapid axonal transport in mammalian neurones. | 1. rabbit peroneal nerves were incubated in vitro in two-compartment chambers. step-gradients of temperature were established so that the proximal part of each nerve was slightly warmer than the distal part. after incubation, the distribution of dopamine-beta-hydroxylase (dbh) activity along the nerves was examined as an indication of the behaviour of rapid transport in adrenergic axons. 2. with temperature gradients of 5 and 8 degrees c, transport velocity in the proximal regions was expected f ... | 1979 | 90719 |
| lymphocyte viability and function following ex-vivo splenic perfusions in dogs. | | 1979 | 90722 |
| lymphocyte alteration by procainamide: relation to drug-induced lupus erythematosus syndrome. | sera from 11 (65%) of 17 patients with newly diagnosed procainamide-induced lupus contained cold-reactive lymphocytotoxic antibodies to normal human lymphocytes in titres of 1/2 to 1/128. in contrast, only 3 of 15 patients on long-term procainamide therapy without lupus and 3 of 65 normal men had serum lymphocytotoxic antibodies, none at a titre higher than 1/2. antibody levels in the lupus patients declined quickly after procainamide was stopped, in parallel with their clinical improvement. pro ... | 1979 | 90917 |
| rubella: which vaccine? | | 1979 | 90959 |
| ultrastructural and autoradiographic study of the effects of bleomycin on the interphase nucleus of cultured normal cells. | primary cultures of hepatocytes and epithelial endometrial cells were treated with bleomycin (10 to 200 microgram/ml) for 30 to 300 min. structural changes were studied with a staining method which contrasts ribonucleoproteins. the earliest visible alteration was the accumulation of perichromatin granules in association with the nucleolus. this disturbance was frequently accompanied by modifications in the nucleolar architecture. after larger treatments, the most striking changes were nucleolar ... | 1979 | 89895 |
| modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase inhibitors. | it has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ... | 1979 | 89899 |
| surface characteristics of chicken peripheral b and t lymphocytes defined by specific heteroantisera. | | 1979 | 89975 |
| cell-free synthesis and segregation of beta 2-microglobulin. | beta2-microglobulin has been synthesized in vitro by using a rabbit reticulocyte lysate system and mrna from the mouse tumor cell line el4. the molecule is synthesized as a precursor with an nh2-terminal extension of 19 amino acids: ser-x-ser-val-x-leu-val-phe-leu-val-leu-val-ser-leu-x-gly-leu-tyr-x. the processing and segregation of this peripheral membrane protein are directly comparable to those of secretory proteins and integral membrane proteins: addition of dog pancreas microsomal membrane ... | 1979 | 91168 |
| the chemistry of the brain. | | 1979 | 91194 |
| anionic sites in the glomerular basement membrane. in vivo and in vitro localization to the laminae rarae by cationic probes. | cationized ferritin (cf) of narrow pi range (7.3-7.5) and the basic dye ruthenium red (rr) have been used as cationic probes to partially characterize anionic sites previously demonstrated in the glomerular basement membrane (gbm). when cf was given i.v. to normal rats and the left kidney was fixed by perfusion 15 min thereafter, clusters of cf molecules were found throughout the lamina rara interna (lri), lamina rara externa (lre), and mesangial matrix distributed at regular (approximately 60 n ... | 1979 | 90048 |
| a study of the muscle damage caused by the 7.62 nato rifle. | | 1979 | 91287 |
| immunological studies of the embryonic muscle cell surface. antiserum to the prefusion myoblast. | xenogeneic antisera raised in rabbits have been used to detect compositional changes at the cell surfaces of differentiating embryonic chick skeletal muscle. in this report, we present the serological characterization of antiserum (anti-m-24) against muscle tissue and developmental stage-specific cell surface antigens of the prefusion myoblast. cells from primary cultures of 12-d-old embryonic chick hindlimb muscle were injected into rabbits, and the resulting antisera were selectively absorbed ... | 1979 | 90049 |
| the zinc-iodide-osmium technique in studies of the neurosecretory system in rats. | | 1979 | 91310 |
| selective vascular thrombosis induced by a direct electrical current: animal experiments. first clinical trials. | | 1978 | 90140 |
| fine specificity of a continuously growing killer cell clone specific for h-y antigen. | h-y-specific cytotoxic t cells were first cloned in soft agar and grown over a period of 8 months in media conditioned with supernatants from mouse and rat spleen cells stimulated with concanavalin a. the specificity of cloned cells and their cytolytic potential remained essentially unchanged over the entire culture period. in addition to lysing male target cells expressing h-2db antigens, the cytolytic cells lysed also male as well as female cells expressing h-2dd alloantigens. seventeen out of ... | 1979 | 91527 |
| morphochemic studies on histamine and monoaminooxidase in the liver and studies on histaminolytic activity of guinea pig plasma in the course of sensitization with heterogenic protein. | | 1979 | 91564 |
| identification of the primate papovavirus hd as the stump-tailed macaque virus. | the recently isolated primate papovavirus hd is shown to be indistinguishable from the stump-tailed macaque virus by immunofluorescent reactivity, by restriction endonuclease analysis, and by nucleic acid hybridization assay. | 1979 | 90163 |
| further studies of the antigenic properties of h3n2 strains of influenza a isolated from swine in south east asia. | h3n2 strains of influenza a isolated from swine in hong kong were compared with human strains of h3n2 influenza a variants in reciprocal hi tests using ferret sera. one isolate from swine was indistinguishable from a/hong kong/68, one set of viruses isolated in 1976 and 1977 was most related to a/hong kong/68 but was not identical to it, two isolates from 1976 were 'bridging strains' that cross-reacted equally with the contemporary variants a/victoria/3/75 and a/texas/1/77, similarly to a small ... | 1979 | 91663 |
| decreased axonal flux of retrogradely transported glycoproteins in early experimental diabetes. | | 1979 | 91664 |
| [method of obtaining a y. pestis fraction containing heterogenetic antigens]. | | 1979 | 91715 |
| decline in hypernatraemia. | | 1979 | 91864 |
| a canine model for the study of allergic asthma and suppression of hapten-specific ige antibody response. | | 1979 | 91961 |
| agarose isoelectric focusing of native human immunoglobulin m and alpha 2-macroglobulin. | the resolution of native 19s igm and alpha 2-macroglobulin by agarose isoelectric focusing is described. the agarose used was practically charge free, thus avoiding disadvantages of electroendoosmosis, and gives a very large network gel, with minimal molecular sieving effects. the resolving power is comparable to that obtained in thin-layer isoelectric focusing in polyacrylamide gel. clones of human igm showed a microclonal heterogeneity, similar to igg antibody heterogeneity, alpha 2-macroglobu ... | 1979 | 89171 |
| serological cross-reactions of leptospiral lipopolysaccharide (f4) antigen. | the serological specificity of leptospiral lipopolysaccharide (f4) antigen was examined by the technique of passive haemagglutination. f4 extracted from leptospiral serovars representative of several different serogroups showed wide cross reaction between serovars, including numerous one-way (non-reciprocal) reactions. the pattern of cross reaction was different to that of the standard leptospiral classification scheme. | 1979 | 92115 |
| viability assessment by dye exclusion. a fluorescent method for fungal cells. | a new fluorescent staining technique for fungi utilizes the differential affinity of dead cells for rhodamine b. a mounting medium has been devised that includes the dye. this medium allows direct processing of cells from a broth and provides optimal conditions for fluorescence intensity. comparison with the standard methylene blue exclusion test as applied to the candida albicans yeast phase indicates similar specificity. various pseudohyphae and several fungi also exhibited selective uptake of ... | 1979 | 92216 |
| [orthosympathetic stimulation after hypercapnia in the rat]. | inhalation of a gas mixture : co2, 25% -- o2, 37.5% -- n2, 37.5%, provokes regularly in the light anaesthetized rat, an increase of catecholaminemia of central origin, associated with systemic arterial hypertension. | 1979 | 92225 |
| processing of 19 s thyroglobulin in vitro [proceedings]. | | 1979 | 92247 |
| shielding of phospholipid vesicles from phospholipase c hydrolysis by alpha-lactalbumin adsorption [proceedings]. | | 1979 | 92261 |
| the antagonistic effect of dexamethasone and insulin on alpha-fetoprotein secretion by cultured h4-ii-e-c3 cells derived from the reuber h-35 hepatoma. | | 1979 | 92316 |
| definition of cellular immune responses to brain antigens in human head trauma. | cellular immune responses to brain antigens in patients with head injury were studied by applying the leukocyte adherence inhibition (lai) assay. the investigation was conducted in three phases. 1) in the initial phase, evaluation of a series of 22 test and 25 control cases obtained at random during a 2- to 6-week time frame following a traumatic event indicated significant non-adherence of leukocytes (nal) in 77% of the test group and 20% of the control group in the presence of brain antigen. 2 ... | 1979 | 89190 |
| clinical aspects of alpha 1-fetoprotein determination in human liver cancer and in humans and experimental animals at carcinogenic risk. | recent findings concerning the significance of alpha 1-fetoprotein (afp) as a tool for clinical diagnosis and monitoring of tumor diseases are reviewed briefly. the applicability of this protein marker to the early diagnosis of patients at carcinogenic risk is discussed. in addition, experimental data obtained with a model of chemical hepatocarcinogenesis are reported. the increase of proliferative activity in precancerous liver tissue preceded afp production under experimental conditions with a ... | 1979 | 89199 |
| antigenic analysis of human heart tissue--characterization of cardiac antigens. | the human heart antigens demonstrated in citric acid extracts by immunodiffusion were submitted to further analysis. heart antigens were precipitated at 30% to 70% saturated ammonium sulphate. these fractions contained antigens reactive with rabbit antisera to human heart. the third fraction out of four proteins which were separated by sephadex g-150 gel filtration, was reactive against rabbit antisera to human heart. the intensely stained proteins of both heart antigens were located at the nine ... | 1979 | 89208 |
| populational polymorphisms in silver staining of nucleolus organizer regions (nors) in human acrocentric chromosomes. | the ag stainability of the nucleolus organizer region (nor) was studied in the acrocentric chromosomes identified by q banding of cultured lymphocytes in 41 karyotypically normal persons (33 males and 8 females) originating from southeast estonia. the data obtained are compared with those established earlier for a combined vienna-ulm population of 51 karyotypically normal persons (see mikelsaar et al., 1977a). significant differences between the two populations in the frequency and patterns of a ... | 1979 | 92452 |
| nonspecific light loss and intrinsic dna variation problems associated with feulgen dna cytophotometry. | nonspecific light loss by the cell-wall-plus-cytoplasm (cwc) can cause a 50% increase in feulgen absorption units in peanut root-tip nuclei as determined by scanning at 450 nm, whereas this phenomenon is not evident with chicken erythrocytes. a two wavelength scanning method of subtracting nonspecific 450 nm absorption from 550 nm feulgen absorption values eliminated the nonspecific light loss in cwc, however, the two wavelength scanning method is time consuming and somewhat impractical with a r ... | 1979 | 92496 |
| ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression. | the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ... | 1979 | 92523 |
| [change in the thresholds of development of ventricular arrhythmias after stimulation of negative emotiogenic hypothalamic centers]. | the dynamics of changes in the thresholds of the origin of ventricular extrasystole, paroxysmal ventricular tachysystole and ventricular fibrillation in long-term stimulation of the ventromedial hypothalamic nucleus were studied in experiments on rabbits. it was found that myocardial resistance to the development of arrhythmias depended on the manifestation of the sympathetico-parasympathic interaction on the heart. in predominance of parasympathetic effects on the heart during hypothalamic stim ... | 1979 | 89222 |
| [method of differentiating k. ozaenae and k. rhinoscleromatis by means of a biotest]. | | 1979 | 92603 |
| olivocerebellar projections in the cat studied by means of anterograde axonal transport of labelled amino acids as tracers. | | 1979 | 92769 |
| capillary permeability to interstitial microinjections of macromolecules and influence of capillary hydrostatic pressure on endothelial ultrastructure. | the transcapillary flux of horseradish peroxidase (hrp, mol. diam. 50--60 a) and ferritin (mol. diam. 110--120 a), microinjected interstitially into the biceps femoris muscle of rats, was analyzed in the electron microscope. from 1 min postinjection hrp was observed to occupy endothelial plasmalemmal vesicles in all positions to be expected if a vesicular transendothelial transport of material occurs in the interstitium-to-lumen direction. permeation of hrp through the intercellular clefts of th ... | 1979 | 89786 |
| isolation and cell culture propagation of rotaviruses from turkeys and chickens. | rotaviruses were detected by electron microscopy in the faeces of turkey poults and broiler chickens with diarrhoea. apparently symptomless infection was also observed in broilers. the avian rotaviruses could not be isolated in cell cultures by conventional techniques, but were adapted to serial growth in chick cell cultures following trypsin treatment of the virus and the cells. immunofluorescence studies showed that the avian and mammalian rotaviruses are antigenically related. antibodies to r ... | 1979 | 92979 |
| ultrastructure of rat renal tubular basement membrane--meshwork structure demonstration by negative staining. | the tubular basement membrane (tbm) (i.e. tubular basal lamina) of rat kidney was shown to be a fine meshwork by electron microscopy after negative staining. strands of the meshwork formed a regular three dimensional lattice work. the pores of the meshwork were polygonal. there were two main pore sizes: one approximately 30 a in diameter, the other 42--60 a. in view of our previous observation that glomerular and alveolar basement membranes were made up fine meshwork, it is quite possible that t ... | 1979 | 89787 |
| presence of human milk particle--specific antigens in malignant human breast tumor tissues. | | 1979 | 93088 |
| effect of spermine on number of crossing-over events in post-conjugational recombination in escherichia coli k-12. | | 1979 | 89796 |
| a radioimmunoassay for erythropoietin. | we describe the development of a radioimmunoassay (ria) for erythropoietin. antisera were raised in rabbits with an impure human urinary erythropoietin preparation used as immunogen, but with pure human erytropoietin serving as the labeled antigen in the ria and as a primary standard. the immunoreactivity of erythropoietin is not altered significantly by the mode of labeling with radioiodine, even though the biologic activity is lost. with this method, it is possible to detect 2--3 mu of erythro ... | 1979 | 89877 |
| use of pm-2 dna degradation as a pharmacokinetic assay for bleomycin. | the pm-2 dna fluorescence assay has been shown to be a rapid, sensitive, and reproducible assay for bleomycin biochemical activity. the assay can detect bleomycin in human serum in the nmol range. the method measures dna degradative activity of bleomycin and could be used to determine activity of bleomycin analogs and metabolites. the usefulness of the assay to perform bleomycin pharmacokinetic studies in cancer patients has been demonstrated. linear regression analyses of parallel bleomycin ass ... | 1979 | 89893 |
| spinal evoked potential in man: a maturational study. | summated evoked potentials to peroneal nerve stimulation which arise in the cauda equina and spinal cord afferent pathways were recorded from surface electrodes placed over the spine of 95 infants, children and adults. the conduction velocity of these potentials from lumbar to cervical recording sites was calculated. additionally, segmental conduction velocities over peroneal nerve, caudal and rostral spinal cord were determined. in all age groups the speed of conduction up the spine was non-lin ... | 1979 | 88331 |
| nucleolar organizing regions of human chromosomes. | silver-stained cells from 49 parents with a history of several abortions were compared with cells from 35 parents with normal liveborn children. the modal and mean number of silver-stained nors (ag-nors) observed on d- or g-group chromosomes was similar in both groups and between males and females. ag-nors were randomly distributed on all five acrocentric pairs. the distribution and size of ag-nors within an individual was not random and was fairly consistent from cell to cell. the mean number o ... | 1979 | 88410 |
| diagnostic dilemma of a 13-month-old boy with 'late-onset' combined immunodeficiency. | a 13-month-old boy had a "late-onset" form of combined immunodeficiency and a fulminant pneumocystis carinii pneumonia of one month's duration. there was no evidence of cutaneous-delayed hypersensitivity responses to diphtheria-tetanus toxoids, candida albicans, or streptokinase-streptodornase, or of lymphocyte dna synthesis after in vitro stimulation with the mitogens phytohemagglutinin and concanavalin a, and only 2% to 4% of peripheral blood e-rosetted t lymphocytes. the serum igm level was n ... | 1979 | 88898 |
| quantitation of skin bacteria: lethality of the wash solution used to remove bacteria. | a widely used technique for the quantitative removal of bacteria from the skin uses a detergent, triton x-100 (p, t-octylphenoxynonaethoxyethanol), to remove and suspend the bacteria. we determined the half-life for the survival of five common skin bacteria suspended in the solution. the shortest-lived was streptococcus pyogenes with a half-life of 0.9 hours. all of the others (propionibacterium acnes, staphylococcus epidermidis, staphylococcus aureus, and klebsiella pneumoniae) had half-lives o ... | 1979 | 93373 |
| viruses and human cancer. a puzzle with a few missing pieces. | | 1979 | 93407 |
| direct visualization of collagens and procollagens in polyacrylamide gels. | | 1979 | 93420 |
| in vitro production of rauscher murine leukemia virus: influence of culture age on biological properties. | large-scale production and concentration procedures have been standardized to study the biological properties of rauscher leukemia virus produced from the high-passaged jls-v9-h mouse bone marrow cell line. virus produced early (days 4 to 6) in the harvest and refeed cycle contained higher levels of ribonucleic acid-directed deoxyribonucleic acid polymerase activity and was more infectious than rauscher leukemia virus produced later (days 7 to 10) in the growth period. the peak of virus producti ... | 1979 | 93428 |
| [cis expression, at complementation, of mitochondrial mosaic gene exons regulating cytochrome b in saccharomyces cerevisiae (proceedings)]. | | 1979 | 93454 |
| a novel class of schizosaccharomyces pombe mutants, phenotypically unable to grow on glycerol, a respiratory substrate, but still able to oxidize glycerol aerobically [proceedings]. | | 1979 | 93469 |
| pineal vasotocin and sleep: involvement of serotonin containing neurons. | | 1979 | 93502 |
| isolation and immunological reactivity of soluble fractions from rat seminiferous tubule basement membrane. | a preparation rich in basement membranes isolated from rat testes (stbm) was exposed to pepsin, collagenase, trypsin, and pronase to obtain soluble fractions. the immunological reactivity of these fractions was studied by gel immunodiffusion or by passive hemagglutination tests against an anti-stbm serum. all fractions reacted with the antiserum, but the highest titer was detected when the antiserum was reacted with a fraction that contained only traces of hydroxyproline (fraction 1), whereas lo ... | 1979 | 90490 |
| purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity. | a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ... | 1979 | 90677 |
| [purification and characterization of the free secretory piece of human colostrum (author's transl)]. | the free secretory piece is isolated from human colostrum by gel filtration and ion-exchange chromatography in high yield (200 mg/l colostrum). deae-cellulose chromatography separates the free secretory piece in two fractions which are electrophoretically distinct, but otherwise have the same characteristics, like molecular weight, antigenic determinants, n-terminal sequence, peptide map and amino acid composition. it was therefore concluded that the protein part of the secretory piece is homoge ... | 1979 | 93565 |
| human beta-thromboglobulin inhibits pgi2 production and binds to a specific site in bovine aortic endothelial cells. | | 1979 | 91112 |
| stationary potential of the brain: part i. basic studies. | | 1979 | 91122 |
| chlorpyrifos and 3,5,6,-trichloro-2-pyridinol: residues in the body tissues of cattle wearing chlorpyrifos-impregnated plastic ear tags. | | 1979 | 93609 |
| [efficacy of plasma substitutes of different molecular weight in acute haemorrhagic shock in dogs (author's transl)]. | dogs were bled into haemorrhagic shock. they were then given isovolaemic infusions of dextran 60 and 40 and of hydroxyethyl starch with an average molecular weight of 200,000 and 40,000 respectively with a view of assessing the haemodynamic efficacy of these plasma substitutes. solutions of high molecular weight hydroxyethyl starch (hes 400) were retained in the circulation for about the same length of time as was dextran 60. hes 40 (molecular weight 40,000) was retained for about 3-4 hours as m ... | 1979 | 91161 |
| characterization of reverse transcriptase from filine leukemia virus by radioimmunoassay. | | 1979 | 91258 |
| lymphocyte transformation in the presence of myelin basic protein in multiple sclerosis and control subjects. | the transformation of lymphocytes in vitro in the presence of human myelin basic protein has been investigated in normal people, patients with multiple sclerosis (ms) and controls with other neurological diseases. there was little or no response at low concentrations (1--10 microgram/ml) but significant transformation at higher concentrations (100--1000 microgram/ml) in all three groups. there was no significant difference among the groups as a whole, but those ms patients who had had disease fo ... | 1979 | 91302 |
| 1-isoamyl-3-isobutylxanthine: a remarkably potent agent for the potentiation of norepinephrine, histamine, and adenosine-elicited accumulations of cyclic amp in brain slices. | | 1979 | 93674 |
| connectivity of the auditory forebrain nuclei in the guinea fowl (numida meleagris). | injection of tritiated leucine and proline into the nucleus ovoidalis of the guinea fowl (numida meleagris) produces terminal labeling in the palaeostriatum and in three adjacent zones (field l1-l3) of the auditory neostriatum (an). l2, situated between l1 and l3, receives the main input and corresponds to the former field l of rose. these neuroanatomically defined zones of the auditory neostriatum are also characterized by differing properties of their neurons. injection of radioactive material ... | 1979 | 91442 |
| [action of feeding lindane or/and zineb on aflatoxin b1-induced toxic effects in rat and mouse liver (author's transl)]. | the action of the ingestion of balanced diets containing pesticides [lindane (125 ppm) or/and zineb (5200 ppm)] on aflatoxin b1-induced toxic effects (inhibition of rna and protein syntheses in liver) has been determined in two animal species. in the rat, the ingestion of these diets for 4 weeks before aflatoxin b1 treatment (0,7 mg/kg for 3 h), strongly decreased the toxic effects on both transcription and translation. by contrast, in the mouse, the above-mentioned diets did not modify the exte ... | 1979 | 93790 |
| characterization of an antigen-specific factor from a hybrid t-cell line. | | 1979 | 93865 |
| rat liver cytosolic azoreductase. electron transport properties and the mechanism of dicumarol inhibition of the purified enzyme. | | 1979 | 91608 |
| [the effect of cerebral perfusion pressure in cerebral infarction after occlusion of middle cerebral artery in dog (author's transl)]. | | 1979 | 91991 |