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incidence of toxigenic campylobacter strains in south africa.campylobacter strains can produce a heat-labile cytotonic toxin (cton) and various cytotoxins (ctox). of 22 south african campylobacter strains tested, 86% were toxigenic (77% produced cton, 41% produced ctox, and 32% produced both types) and 14% were toxin negative. campylobacter jejuni strains were 67% cton positive and 47% ctox positive, whereas campylobacter coli strains were 100 and 29% positive, respectively.19911864947
enterotoxigenicity of human and animal isolates of campylobacter jejuni in ligated rat ileal loops.human and animal isolates of campylobacter jejuni were tested for enterotoxigenicity in ligated loop of charles-foster rats. of 21 isolates, 13 were proved to be toxin-positive in the initial experiments. however, the remaining 8 required one to three passages through the rat ileal loop before showing the capacity to produce enterotoxin. all isolates caused fluid accumulation comparable with the amount produced by the standard toxigenic strain of vibrio cholerae 01 1naba 569b. the isolates of c. ...19911869798
simultaneous colonization of campylobacter jejuni and salmonella typhimurium in day-old chicks.day-old chicks were challenged with campylobacter jejuni or salmonella typhimurium or both to assess the influence of these two bacteria upon the colonization of one another. median colonization dose (cd50), a measure indicating the number of organisms required to colonize one-half of the challenged chickens, was used to assess the influence of these organisms upon the colonization of one another. chicks were gavaged with serial dilutions of 1) c. jejuni, 2) s. typhimurium, 3) dilutions of c. je ...19911876560
an evaluation of three commercial fecal transport systems for the recovery of enteric pathogens.twenty-five isolates, including six strains of shigella species, six strains of salmonella species, five strains of yersinia enterocolitica, six strains of campylobacter jejuni, and two strains of vibrio parahaemolyticus, were inoculated at a concentration of 1.5 x 10(4) colony-forming units/ml into the following transport systems: fekal enteric plus (trend scientific, inc., st. paul, mn), cary blair transport medium (remel, inc., lenexa, ks), and para-pak c & s (meridian diagnostics, inc., cinc ...19911877533
[distribution of serotypes and biotypes of campylobacter jejuni/coli strains from ten provinces/cities in china].the lior's schemes were used for serotyping and biotyping 301 c. jejuni/coli strains isolated from different hosts in ten provinces/cities of china. no difference was found between the serotype distributions of the strains isolated from various areas. forty-one serotypes were detected. of the isolates, 86.71% were typable and 3 new serotypes, lio n-1, n-2 and n-3, were developed. the differences were proved among the serotype distributions of the isolates from different areas. it was shown that ...19911878957
evaluation of campylobacter jejuni colonization of the domestic ferret intestine as a model of proliferative colitis.forty 3- to 17-week old domestic ferrets, including 2 gnotobiotes, were inoculated orally and/or rectally with 10(6) to 10(9) colony-forming units of 1 or more of 4 strains of campylobacter jejuni, 3 of mink and 1 of human origin. feeding or gavage of any of the 4 strains, in milk or broth, with or without preinoculation sodium bicarbonate treatment to neutralize stomach acid, induced colonization in 38/40 ferrets; diarrhea lasted 2 to 4 days in conventional kits, 6 days in gnotobiotes. bacterem ...19911883085
beta-lactamase production in human and animal isolates of campylobacter jejuni in turkey. 19911885429
identification, purification, and characterization of major antigenic proteins of campylobacter jejuni.evidence from developing countries and volunteer studies indicates that immunity to campylobacter jejuni and campylobacter coli may be acquired, but the antigenic basis for this protection is poorly defined. we have purified to homogeneity four proteins with molecular weights of 28,000 (peb1), 29,000 (peb2), 30,000 (peb3), and 31,000 (peb4) from epidemic c. jejuni strain 81-176 using acid extraction and sequential ion-exchange, hydrophobic interaction, and gel filtration chromatography. the rela ...19911885571
simultaneous infection with multiple serotypes of shigellae in a patient.we isolated three different serotypes of shigella on admission from a patient with dysentery as well as a shigella-like organism and campylobacter jejuni upon follow-up. the patient produced serum antibodies to all three serotypes of shigellae.19911889185
evaluation of 10 methods to distinguish epidemic-associated campylobacter strains.we compared four phenotypic and six genotypic methods for distinguishing campylobacter jejuni strains from animals and humans involved in four epidemics. based on a comparison with epidemiologic data, the methods that correctly identified all strains in three milkborne outbreaks and one waterborne outbreak were heat-stable and heat-labile serotyping; multilocus enzyme electrophoresis (mee); dna restriction endonuclease analysis with bglii, xhoi, pvuii, or psti; and southern blot and hybridizatio ...19911890168
waterborne outbreak of campylobacter jejuni in christchurch: the importance of a combined epidemiologic and microbiologic investigation.campylobacter is a common cause of gastroenteritis in new zealand; however, the source of infection usually remains unknown. reports of two cases of campylobacter jejuni enteritis at a camp and convention centre near christchurch were investigated. through interviews of persons living at or attending the camp, 42 additional cases were identified. epidemiologic and microbiologic data strongly suggested the water supply as the source of infection. the combined epidemiologic and microbiologic inves ...19911891134
inhibition of acid secretion from parietal cells by non-human-infecting helicobacter species: a factor in colonization of gastric mucosa?helicobacter pylori has been shown to produce a protein that inhibits acid secretion from parietal cells. we have examined other non-human-infecting helicobacter species for this property by measuring the uptake of [14c]aminopyrine into rabbit parietal cells as an indirect assessment of acid secretion. helicobacter felis and an isolate from a rhesus monkey were shown to inhibit acid secretion. isolates of helicobacter mustelae gave variable responses. whole bacteria and cell-free sonicates impai ...19911894369
enterotoxigenic escherichia coli associated with infant diarrhoea in galicia, north-western spain.to assess the role of enterotoxigenic escherichia coli (etec) in infantile diarrhoea, 482 children with diarrhoea and 103 healthy controls, from three localities of galicia, north-western spain, were investigated between 1985 and 1988. rotavirus (37.3%) and salmonella spp. (12.8%) were the most common causal agents, followed by etec (3.9%), campylobacter jejuni (2.3%), shigella spp. (0.9%) and yersinia enterocolitica (0.5%). etec were significantly more frequently isolated from children with dia ...19911895327
enteral feeding and infection in the immunocompromised patient.evidence is accumulating that immunocompromised individuals are at an increased risk of infection from foodborne pathogens including campylobacter jejuni, listeria monocytogenes, salmonella spp. normal bacterial flora and contaminants of foods and enteral feeds can also result in nosocomial infection in susceptible individuals. safe food handling, low-microbial diets, and measures to reduce bacterial contamination of enteral foods can reduce exposure to potential pathogens in the food supply.19911906573
comparison of the survival of campylobacter jejuni and campylobacter coli in culturable form in surface water.six campylobacter jejuni and six campylobacter coli strains were isolated from cows and pigs, and their survival in lake water was compared by viable counts. campylobacter jejuni survived longer in culturable form than c. coli in untreated and membrane-filtered water both at 4 and 20 degrees c. this difference in survival time may be a reason why c. jejuni is generally isolated from surface waters more frequently than c. coli. both species survived better in filtered than in untreated water. thi ...19911913357
screening of children for enteric bacterial pathogens in the outborn neonatal ward in lagos, nigeria.babies, on admission into a neonatal ward at the lagos university teaching hospital, had their rectal swab specimens examined bacteriologically and screened for enteric bacterial pathogens over a one-year-period at two-week intervals. it was found that on the average there were 3 (9.68%) enteric bacterial pathogens out of an average of 31 admissions at each screening period. the enteric bacterial pathogens isolated included: non-typhoid salmonellae, which accounted for 55 (80.88%) isolates out o ...19911915794
comparison of preston agar and a blood-free selective medium for detection of campylobacter jejuni in food.the present collaborative study compares recovery of campylobacter jejuni from food in 2 agar media. six laboratories analyzed 8 samples each of chicken liver inoculated with campylobacter jejuni. samples were enriched in preston broth and isolation was carried out on preston agar (pa) and campylobacter blood-free selective medium (cbfs), a charcoal-based medium with cefoperazone and amphoteracin as antibiotic supplements. there was no difference in the recovery rate between the 2 agar media; ho ...19911917811
serologic evidence of campylobacter jejuni/coli enteritis in patients with guillain-barré syndrome.we performed serologic testing for campylobacter jejuni in 17 consecutive patients with acute guillain-barré syndrome from the boston, mass area to compare the frequency of this preceding infection with the high rates reported from other areas of the world. the rate of seropositivity, 18%, was considerable, but it was lower than that reported in australia. moreover, all of our patients with definite serologic evidence of infection had severe enteritis before guillain-barré syndrome, usually with ...19911929902
[campylobacter jejuni: reflections on enteric infection among mexican children]. 19911930712
[campylobacter jejuni infection in children of a rural community].colonization of the intestine with campylobacter jejuni was followed longitudinally from birth in a cohort of 75 rural children with fecal cultures taken every fortnight and every time they had diarrhea. only 25% of children initially colonized with c. jejuni during the first year of life, and 12% of children initially colonized during the second had diarrhea. the age at which a child was initially infected with c. jejuni was not a risk factor in relation with presence of disease. a higher illne ...19911930713
intestinal colonization of rats following experimental infection with campylobacter jejuni.oral infection of rats with 10(6)-10(8) colony forming units per ml of campylobacter jejuni resulted in prompt intestinal colonization. campylobacter jejuni was recovered from rat faecal samples for four months post infection. the colonized rats did not succumb to clinical diarrhoea or pyrexia. the resistance of rats to c. jejuni clinical infection and their ability to shed the organism for protracted periods, make rats potential source of zoonotic campylobacteriosis.19911935728
on the microbiological diagnostics of campylobacter jejuni.investigation of campylobacteriosis cases in 1983-1989 resulted in the isolation of a total of 245 antigenically identified and 23 unidentified strains from humans, animals and foods. a commonly accepted method developed in 1985 using our own experience was used for strain isolation and culturing. a variety of nutrient media in combination with different supplementary substances (antibiotics, growth factors) and additives, such as horse serum, were verified as well as filtration and fortner's pr ...19911940334
characterization of campylobacter jejuni strains occurring in the ussr and the czech republic.in 1986-1989 the microbiological laboratory in prague obtained 100 campylobacter jejuni strains while its counterpart in moscow gained 120 such strains. the strains were primarily isolated from humans with diarrheal disease, from domestic and wild animals and from the environment. most c. jejuni strains were successfully specified and classified using a czechoslovak serotyping scheme proposed by kahlich. serotypes 1, 2, 7, 15, 22 and 24 occurred most frequently in the czech republic whereas in t ...19911940335
prevalence of virulence markers of enteric campylobacter in france and tunisia.forty-nine strains of campylobacter jejuni and c. coli were isolated from the stools of 49 patients clinically documented for diarrhoea and fever, and living either in the paris metropolitan area (30) or in the tunis area (19). the strains were identified biotyped, serotyped and studied for association with hela cells and the ability to elongate chinese ovary cells (cho). the c. jejuni biotype i was more frequent among tunisian strains and the c. jejuni biotype ii was more frequent among french ...19911947431
survival of campylobacter jejuni and pathogenic escherichia coli in mahewu, a fermented cereal gruel.the survival of strains of campylobacter jejuni and enteropathogenic and enterotoxigenic (lt) escherichia coli was investigated in mahewu, a traditional fermented cereal gruel. the mahewu was inoculated with 10(6) to 10(7) colony forming units of the bacterial cells per ml of the cereal gruel. none of the strains of c. jejuni were detected 30 min after inoculation. all the strains of e. coli were detected after 24 h in mahewu but did not show any increase in numbers of surviving cells. the enter ...19911949147
comparative study of colonizing and noncolonizing campylobacter jejuni.campylobacter jejuni a74/o and a74/c are congenic strains. an oral dose of 10(5) organisms of strain a74/c colonizes chicken intestines. strain a74/o, from which a74/c is derived, does not colonize the chicken intestines with an oral dose of 10(5) organisms. in this study, the congenic bacteria were compared to identify possible colonization mechanisms. differences were not observed in plasmid content or by hindiii, pst i, acc i, hincii, ava i, ava ii, xba i, and bamhi restriction enzyme digesti ...19911952344
seasonal effects on the reported incidence of acute diarrhoeal disease in northeast thailand.this paper examines the seasonal variation in the reported incidence of acute diarrhoea for selected areas in the northeast of thailand. charts are presented which show rainfall, temperature and reported incidence of acute diarrhoea for the period 1982 to 1987. incidence of diarrhoea appears to be inversely related to a sharp decrease in temperature around january each year. although rainfall does not appear to have a direct effect on the relative incidence of acute diarrhoea, there is always a ...19911955264
enteric infections associated with exposure to animals or animal products.the epidemiology and clinical presentation of enteric infections are discussed in this article. these include bacterial, viral, and parasitic illnesses, and are associated with the increasing popularity of drinking unpasteurized milk; eating raw fish and shellfish; consuming undercooked pork, poultry, and eggs; and having contact with pets. salmonella, campylobacter jejuni, vibrio, yersinia, aeromonas, edwardsiella, hepatitis a virus, norwalk virus, anisakis, eustrongylides, diphyllobothrium, na ...19911955706
common and variable domains of the flagellin gene, flaa, in campylobacter jejuni.the organization of the flagellin gene locus in campylobacter jejuni strain in1 (lior 7) was determined using the polymerase chain (pcr) reaction and a series of oligonucleotide primers. two tandemly arranged flagellin genes of approximately 1.7 kb were found to be joined by an intervening segment of c.0.2kb, similar to that reported for campylobacter coli. the 5' flagellin gene, flaa, was generated by pcr and both strands sequenced. comparison of the deduced amino acid sequence for c. jejuni fl ...19911956293
[bacterial ileocecitis, a "new" disease].786 patients with suspected acute appendicitis or appendiceal mass were examined by ultrasonography to distinguish appendicitis from bacterial enteritis. 533 of these patients were described before. in 91 (11.6%) ultrasonography revealed the characteristic picture associated with bacterial enteritis of the ileocaecal region-enlarged mesenteric lymph nodes and mural thickening of the terminal ileum and caecum--but no image of an inflamed appendix. in 64 of these a bacterial infection was confirme ...19911956444
survival of escherichia coli and campylobacter jejuni in untreated and filtered lake water.the survival of campylobacter jejuni and escherichia coli in lake water was studied using viable counts. escherichia coli survived better than c. jejuni in all the test conditions studied. both the species survived better in filtered than in untreated water. this suggests that predation and/or competition for nutrients affect the survival of both the species in an aquatic environment. campylobacter jejuni survived less well in filtered autoclaved water and in 0.9% nacl than in filtered water wit ...19911960115
antibiotic sensitivity pattern; experience at university hospital, riyadh, saudi arabia.results of sensitivity testing were discussed based on examination of 5192 isolates of the various bacteria isolated from clinical specimens from king khalid university hospital in riyadh, saudi arabia. streptococcus pyogenes and streptococcus pneumoniae were sensitive to penicillin and erythromycin. the sensitivity pattern of staphylococcus aureus was also predictable as they were fairly sensitive to both methicillin (98%) and erythromycin (96%). neisseria gonorrhoeae (27%) showed a high level ...19911960392
[rotavirus diarrhea in children hospitalized in wrocław clinics].studies have been performed in 250 children aged 0 to 3 years suffering from diarrhoea. rotaviruses have been founded in 25% of cases, most frequently in children aged 0 to 3 months (29.6%), and 6 to 12 months (21.8%). frequency of rotavirus infections was highest during february. in 26% of subjects studied the coexisting infection with enteropathogenic escherichia coli strains or salmonella and campylobacter jejuni has been observed. the rotazyme ii test is more sensitive than the rotalex and r ...19901965233
guillain-barré syndrome and campylobacter jejuni infection. 19901971411
jackdaws as potential source of milk-borne campylobacter jejuni infection. 19901971891
bird attack on milk bottles: possible mode of transmission of campylobacter jejuni to man.a case-control study was carried out to test the hypothesis that the rise in the rate of campylobacter jejuni infection in the brigend area of south wales during may was due to the consumption or handling of milk from bottles that had been attacked by birds. 32 of 36 cases meeting the case definition were interviewed, along with 2 controls per case, matched for age, sex, and area of residence. there were strong associations between campylobacter infection and doorstep delivery of milk bottles, a ...19901978882
restriction fragment length polymorphism for the identification of campylobacter jejuni-isolates.companion animals ("pets") are occasionally carriers of organisms pathogenic for man. in the present, study fecal samples of clinically inapparent animals with direct contact to 204 patients, suffering from campylobacter enteritis, were investigated for c. jejuni or c. coli (cjc). cjc positive animals were seen in the environment of only five patients (= 2.4%). by comparison of biotypes and serotypes of thermostable and thermolabile antigens from human and animal isolates no clear epidemiologica ...19901982490
chemical characterization of campylobacter jejuni lipopolysaccharides containing n-acetylneuraminic acid and 2,3-diamino-2,3-dideoxy-d-glucose.lipopolysaccharides (lps) of four nonencapsulated strains of the human enteric pathogen campylobacter jejuni were chemically characterized. when applied to two of the strains, extraction by a modified phenol-chloroform-petroleum ether method (h. brade and c. galanos, eur. j. biochem. 122:233-237, 1982) gave better yields of lps than did extraction by the conventional hot phenol-water technique. constituents common to all lps were d-glucose, d-galactose, l-glycero-d-manno-heptose, 3-deoxy-d-manno ...19911987154
production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11.a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ...19911993773
measles-associated diarrhea in hospitalized children in lima, peru: pathogenic agents and impact on growth.because the causes of measles-associated diarrhea are not well known, 0- to 5-year-old children presenting to the hospital with measles-associated diarrhea (cases, n = 77) or acute diarrhea only (controls, n = 77) were compared. growth and diarrheal morbidity were evaluated for 1 month after acute illness. campylobacter jejuni was more frequently isolated from cases (31%) than controls (16%; p = .03). rotavirus was absent in all cases versus 28% of controls (p less than .001). incidence density ...19911995722
campylobacter jejuni as a cause of acute diarrhoea in children: a study at an urban hospital in bangladesh.the importance of c. jejuni as an aetiological agent of childhood diarrhoea was investigated at an urban children's hospital in dhaka over a period of 1 year. c. jejuni was isolated from 25.5% of 102 diarrhoeal patients compared to 8.6% of 93 age and sex-matched healthy control children studied (p less than 0.002). the organism was isolated as a single pathogen in 17.6% of diarrhoeal patients. no c. coli was detected. the infection rate was significantly higher (p less than 0.05) amongst childre ...19911995935
shuttle cloning and nucleotide sequences of helicobacter pylori genes responsible for urease activity.production of a potent urease has been described as a trait common to all helicobacter pylori so far isolated from humans with gastritis as well as peptic ulceration. the detection of urease activity from genes cloned from h. pylori was made possible by use of a shuttle cosmid vector, allowing replication and movement of cloned dna sequences in either escherichia coli or campylobacter jejuni. with this approach, we cloned a 44-kb portion of h. pylori chromosomal dna which did not lead to urease ...19912001995
virulence of campylobacter jejuni for chicken embryos is associated with decreased bloodstream clearance and resistance to phagocytosis.the 11-day-old chicken embryo has been shown to be a useful animal model for comparing the virulence of human isolates of campylobacter jejuni. virulence in this system is associated with the ability to invade the chorioallantoic membrane and to survive and proliferate in vivo. in this study, the survival and multiplication of c. jejuni in the embryonic host was investigated. it was possible to enhance the virulence of a relatively avirulent c. jejuni strain by passaging it intravenously through ...19912004823
evaluation of an immunofluorescence assay for specific detection of immunoglobulin g antibodies directed against helicobacter pylori, and antigenic cross-reactivity between h. pylori and campylobacter jejuni.an immunofluorescence assay (ifa) for the detection of immunoglobulin g antibodies directed against helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms. the resulting anti-h. pylori titers were classified as follows: negative, less than or equal to 64; borderline, 128; and positive, greater than or equal to 256. by using these cri ...19912007640
therapy for acute infectious diarrhea in children.this article reviews current recommendations of therapy with antidiarrheal compounds and antimicrobial agents for acute infectious diarrhea in children. in most infants and children with acute infectious diarrhea, treatment with antidiarrheal compounds is not indicated. many of these compounds interfere with identification of enteropathogens in stool specimens, and the antimotility class has an overdose potential. antimicrobial therapy is given to reduce symptoms and to prevent the spread of inf ...19912007952
an outbreak of campylobacter jejuni gastroenteritis linked to meltwater contamination of a municipal well.a large outbreak of campylobacter jujuni gastroenteritis attributed to contamination of an unchlorinated municipal water system was investigated. unlike most previous summer outbreaks, this one began in early spring and was attributed to meltwater entering one or more municipal wells. 241 suspected cases were documented, but retrospective information from local health care workers suggested a much larger outbreak. 45 laboratory-confirmed cases participated in a case-control study which showed a ...19912009481
campylobacter: pathogenicity and significance in foods.in the last 10 years campylobacter jejuni has emerged as the most frequent cause of bacterial gastroenteritis in man. acute enterocolitis, the most common presentation of c. jejuni infection, can affect persons of all ages. c. jejuni has been found in virtually every country where investigations have been carried out. the frequent finding of dysenteric stools suggests that mucosal damage due to an invasive process analogous to that seen in shigellosis is important in the pathogenesis. campylobac ...19912018702
the use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases.the application of nucleic acid analyses to investigations of infectious disease outbreaks has resulted in useful molecular strain markers that distinguish the epidemic clone of a particular pathogen and help identify specific vehicles of infection. we have successfully used plasmid profile analysis, restriction endonuclease digestion of plasmid and whole-cell dnas, and nucleic acid hybridization to investigate recent outbreaks of foodborne diarrheal illness. plasmid analysis has been important ...19912018708
isolation of group 2 aerotolerant campylobacter species from thai children with diarrhea.campylobacter species were isolated from 93 (15%) of 631 thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees c. campylobacter jejuni was isolated from 62 (10%), campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). the 17 atypical strains were first characterized biochemically and by dot blot dna hybridization. catalase-negative strains also were characterized by dna hybridization and ribotype pattern. one strain ...19912019754
pulsed-field gel electrophoresis of campylobacter jejuni and campylobacter coli genomic dna and its epidemiologic application.genomic dna from 12 different campylobacter jejuni and 10 campylobacter coli isolates was digested with smai and analyzed by pulsed-field gel electrophoresis (pfge) and 16s rrna hybridization studies. although the two campylobacter species displayed species-specific restriction and hybridization patterns, significant intraspecies differences were observed. combined pfge and hybridization pattern analysis failed to provide any more epidemiologic information than was obtained from pfge restriction ...19912019755
comparison of selective medium supplemented with blood & a charcoal-based blood-free medium for primary isolation of campylobacters from human faeces.a recently developed charcoal-based, blood-free selective medium-charcoal cefoperazone deoxycholate agar (ccda) was compared with the conventional butzler's agar (ba) for primary isolation of enteric campylobacters from human stool specimens. ccda yielded higher percentage (93.6%) of positive isolations as compared to ba (76.6%). the rate of isolation on ccda was significantly higher (p less than 0.0001) than on ba. cost-wise also, the ccda medium was two times cheaper than ba. in addition to ca ...19912022396
better preservation of campylobacter jejuni/c. coli in a defined medium.campylobacter jejuni and c. coli strains were preserved at -10 degrees c in different stock media to determine their efficacy to preserve the organism for a longer period of time. an improved defined stock culture medium was developed for the organism by removal and effective neutralisation of the toxic metabolites. comparative study revealed that phosphate buffer saline (pbs), ph 6.7 supplemented with 0.2 per cent charcoal, 0.025 per cent fbp (ferrous sulphate, sodium metabisulphate and sodium ...19912022397
early onset neonatal sepsis with campylobacter jejuni: a case report.perinatal infections with bacteria belonging to the genus campylobacter are being increasingly recognized. we present a case of early onset neonatal sepsis with campylobacter jejuni (previously c. fetus ss. jejuni or vibrio jejuni). the infant was born prematurely at 31 weeks of gestation and presented with respiratory distress and frequent apnoea from birth. the chest x-ray film demonstrated reticulogranular pattern consistent with hyaline membrane disease. the infant was successfully treated w ...19912029921
lansoprazole, a novel benzimidazole proton pump inhibitor, and its related compounds have selective activity against helicobacter pylori.the activities of various types of antiulcer agents against helicobacter pylori (formerly called campylobacter pylori) strains were determined by an agar dilution method. among the compounds tested, two benzimidazole proton pump inhibitors, lansoprazole (ag-1749) and omeprazole, were found to have significant activities against this organism. the activity of lansoprazole was comparable to that of bismuth citrate, with mics ranging from 3.13 to 12.5 micrograms/ml, and fourfold more potent than th ...19912039199
structural analysis of the lipid a component of campylobacter jejuni ccug 10936 (serotype o:2) lipopolysaccharide. description of a lipid a containing a hybrid backbone of 2-amino-2-deoxy-d-glucose and 2,3-diamino-2,3-dideoxy-d-glucose.the chemical structure of campylobacter jejuni ccug 10936 lipid a was elucidated. the hydrophilic backbone of the lipid a was shown to consist of three (1----6)-linked bisphosphorylated hexosamine disaccharides. neglecting the phosphorylation pattern, a d-glucosamine (2-amino-2-deoxy-d-glucose) disaccharide [beta-d-glucosaminyl-(1----6)-d-glucosamine], a hybrid disaccharide of 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine [2,3-diamino-2,3-dideoxy-beta-d-glucopyranosyl-(1----6)-d-glucosamin ...19912040305
bacterial antibodies in ankylosing spondylitis.antibodies to salmonellae, yersiniae, campylobacter jejuni, borrelia burgdorferi, klebsiella pneumoniae, escherichia coli, proteus mirabilis and chlamydia trachomatis were measured by elisa in the sera of 99 patients with ankylosing spondylitis. increased prevalence of iga and igg class antibodies against k. pneumoniae and of iga class against e. coli was observed in ankylosing spondylitis. no clear correlation between the disease activity and occurrence of antibodies was revealed. the results a ...19912044228
waterborne campylobacteriosis in northern norway.a suspected waterborne outbreak of presumed campylobacteriosis involving approximately 680 of the 1000 inhabitants in a community is described. twenty-two strains of campylobacter jejuni were isolated from patients. ten randomly chosen isolates were identified as biotype 1 lau 0:1 (n = 9) and biotype 2 pen 0:6, 7 (n = 1) and one from tap water as biotype 1 pen 0:19, 22. the majority of cases occurred within a period of 1 week. examination of human sera obtained during the first 2 weeks of the ep ...19912049283
effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products.pork slurries inoculated with various test microorganisms were prepared and subjected to high hydrostatic pressure at 1000 to 6000 atm for 10 min at 25 degrees c to examine for the pressure effects on characteristics of the slurries and the inactivation of the microorganisms associated with meat and meat products. pressure treatment at higher than 3000 atm caused coagulation and discoloration of the pork slurries. harder and more white coagulants were obtained by increasing the pressure. pressur ...19912049285
haemolytic anaemia after childhood escherichia coli o 157 .h7 infection: are females at increased risk?we conducted a 4-year retrospective cohort study to better define the risk of haemolytic anaemia and haemolytic uraemic syndrome (hus) in children following sporadic gastrointestinal infection with the o 157.h7 serotype of escherichia coli. of the 72 children infected with this organism, 9 (12.5%) developed haemolytic anaemia, 6 of whom had hus. no child in a cohort of 72 age-matched controls with campylobacter jejuni gastroenteritis developed haemolytic anaemia (p = 0.003). females had a signif ...19912050207
study on the pathogenicity of campylobacter jejuni by modifying the medium.campylobacter jejuni has been documented as one of the major aetiological agents of diarrhoeal illness all over the world. studies revealed its pathogenicity by different assay methods, but none could be strongly recommended as a tool for differentiating toxigenic strains of c. jejuni. this study was an attempt to demonstrate better its pathogenicity by media modification. fifteen isolates of c. jejuni recovered from diarrhoeal patients at the international centre for diarrhoeal disease research ...19912051523
comparison of six media, including a semisolid agar, for the isolation of various campylobacter species from stool specimens.a recently described semisolid blood-free selective motility medium (ssm) (j. goossens, l. vlaes, i. galand, c. van den borre, and j. p. butzler, j. clin. microbiol. 27:1077-1080, 1989) was compared with two charcoal-based selective media (charcoal-based selective medium [csm] and modified charcoal cefoperazone deoxycholate agar [ccda]), two blood-based media (skirrow medium [skm] and campybap), and a passive, 0.65-microns-pore-size cellulose acetate membrane filter technique for the recovery of ...19912056033
effect of iron-dextran on lethality of nigerian isolates of campylobacter jejuni in mice.mice were orally and intragastically challenged with standard suspensions containing 1 x 10(9-10) colony forming units (c.f.us) per ml of strains "2" and "5" of campylobacter jejuni isolated in lagos. another group of mice received the same amount of c. jejuni and 1pc iron-dextran which was incorporated into the bacterial suspension ad mueller-hinton broth. two sets of control mice were included in the study. one set received only 0.2 ml of phosphate buffered saline while the second set received ...19912060004
complete sequence of the campylobacter jejuni glya gene encoding serine hydroxymethyltransferase.the complete nucleotide sequence of the campylobacter jejuni glya gene was determined and the amino acid (aa) sequence of its product, serine hydroxymethyltransferase (shmt), was deduced. the deduced polypeptide has 414 aa residues (mr 45,758). the aa sequences of c. jejuni and escherichia coli show 55.6% identity. comparative analysis of the aa sequences of the shmts of e. coli and c. jejuni identified two new putative functional domains. the translational product of the c. jejuni glya gene was ...19912060796
inactivation of campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaa but not flab is required for invasion.the role of the campylobacter jejuni flagella in adhesion to, and penetration into, eukaryotic cells was investigated. we used homologous recombination to inactivate the two flagellin genes flaa and flab of c. jejuni, respectively. mutants in which flab but not flaa is inactivated remain motile. in contrast a defective flaa gene leads to immotile bacteria. invasion studies showed that mutants without motile flagella have lost their potential to adhere to, and penetrate into, human intestinal cel ...19912065653
in vitro activity of tosufloxacin against bacterial enteric pathogens.the in vitro activity of tosufloxacin (a-61827), a new quinolone antibiotic, was compared with that of four other quinolones against 162 bacterial enteric pathogens. susceptibility testing was performed by using an agar dilution method. the minimal inhibitory concentration (mic) was defined as the lowest concentration of antibiotic without visible growth. tosufloxacin was the most active agent against campylobacter jejuni and c. coli, and ciprofloxacin was the most active agent against salmonell ...19902076595
six enteropathogens isolated from a case of acute gastroenteritis.isolation of six different gastrointestinal pathogens (entamoeba histolytica, plesiomonas shigelloides, campylobacter jejuni and three different salmonella species [salmonella typhimurium, salmonella blockley and salmonella hadar]) in the feces of a german female tourist suffering from acute diarrhoea after a trip to bali.19902076911
[infantile diarrheal diseases in madagascar: bacterial, parasitologic and viral study].from november 1988 to october 1989, an etiological study showed off the prevalence and the part of several enteropathogen agents which are not yet studied in madagascar. 1,523 stool's samples from 884 children with diarrhea and 639 children without diarrhea from 0 to 14 years old have been investigated. a bacterial, parasitical or viral etiology was found from 36.3% of diarrheic children and 11.2% of healthy children. the three agents the most frequently identified from children with diarrhea ar ...19902078082
identification of campylobacter jejuni and campylobacter coli from waste water by sds-disc-page of whole cell protein.analysis of the electrophoretic profiles of whole cell proteins of campylobacter strains from waste water was used to differentiate c. jejuni from c. coli. the results show, that biotyping of campylobacters from waste water may results in a false species differentiation. some of the campylobacter strains originally typed as c. coli, were proved to be atypical c. jejuni strains. therefore, in addition to biotyping and serotyping, electrophoretic typing is recommended to ensure correct identificat ...19902080967
[evaluation of a latex agglutination method for the rapid identification of campylobacter jejuni/coli].a latex agglutination assay was developed to identify campylobacter jejuni and campylobacter coli. we evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) to prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 mcfarland unit, and heated at 121 degrees c for 10 to 30 min. 2) bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without ...19902082036
[value of microbiologic studies for diagnosis of post-enteritis reactive arthritis].reactive arthritis may develop within a period of some days until upto 3 weeks after infections with yersinia enterocolitica, yersinia pseudotuberculosis, campylobacter jejuni/coli, shigella and salmonella. intestinal infections with klebsiella pneumoniae, clostridium perfringens, clostridium difficile, cryptosporidium, strongyloides stercoralis, taenia saginata and schistosoma mansoni are, in some cases, considered to be responsible for reactive arthritis. detection of pathogenic bacteria in fe ...19902085058
campylobacter jejuni peritonitis during chronic ambulatory peritoneal dialysis (capd).a patient treated with chronic ambulatory peritoneal dialysis developed recurrent peritonitis. during a fourth episode, campylobacter jejuni was cultured from the dialysate. she responded well to streptokinase and imipenem.19902085604
aetiology of bacterial diarrhoea in north indian children with special reference to campylobacter jejuni.a total of 184 cases of acute diarrhoea, in patients aged 1-36 months, were studied clinicobacteriologically. enteropathogens were isolated in 22.28% cases. campylobacter jejuni was isolated from 2.17% cases only. salmonellae were isolated from 7.6% cases and 64.28% of them were s typhimurium. more than 75% of all isolates of salmonellae, shigellae, esch coli and campylobacter jejuni were sensitive to gentamicin and cephaloridine. secondary lactose intolerance was noted in 6.52% cases only.19902090681
[a 3-year follow-up study of the incidence of campylobacteriosis in a pediatric population].the authors investigated the incidence of campylobacteriosis in the population of five paediatric health communities of the jihlava policlinic (5831 children) for a period of three years. a total of 2408 specimens faeces from 1501 subjects were examined. campylobacter jejuni was detected in 151 subjects (10.1%), salmonella sp. in 47 (3.1%), shigella sp. in 18 (1.2%), yersinia enterocolitica in 12 (0.8%) and enteropathogenic escherichia coli in five subjects (0.3%). the total incidence of campylo ...19902092913
laboratory findings associated with abomasal ulcers/tympany in range calves.the etiology of abomasal ulcers/tympany was investigated in 48 animals from 36 ranches in wyoming and nebraska. results indicate that subclinical trace mineral deficiencies of copper and/or selenium exist in the range cattle in west central nebraska and wyoming. etiological agents most frequently incriminated by bacteriologic cultures and/or histopathic examination were clostridium perfringens and campylobacter species. histopathologic evaluation of abomasums revealed 31 of 38 cases contained ab ...19902094446
[comparative study of campylobacter pyloridis and campylobacter jejuni under electron microscope].specimens were made by preparing the ultrathin sections after negative staining and ruthenium red staining. the specimens were observed under electron microscope. results revealed that the shapes of campylobacter pyloridis (cp) and c. jejuni (cj) were similar, but distinctive differences in their structures were observed. cp had one to five sheathed flagella at one end with bulbous tips. cj had only one unsheathed flagellum on each end without a bulbous tip. the cell body of cp was longer than t ...19902094635
[primary study on pathogenesis of campylobacter jejuni enteritis].the invasiveness and heat-labile enterotoxin of 11 human strains of c. jejuni were detected in nih mice inoculated with the c. jejuni strains; the methods of plate immunological hemolysin test and spa-coagglutination test were employed. ten strains of the bacteria invaded the intestine; 7 strains (45.46%) were positive in spa-coagglutination test; and 4 (36.36%) produced enterotoxin. c. jejuni in chengdu area possess multiple pathogenicity, e.g invasiveness and heat-labile enterotoxin, but we di ...19902094644
immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis.antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ...19902099622
the incidence and epidemiological significance of campylobacter jejuni/coli serotypes in romania.the distribution per serotypes of 401 campylobacter strains was studied by lior's procedure, 241 strains being identified as c. jejuni (60.1%) and 160 as c. coli (39.9%): out of these 329 were of human origin (82%), including 225 c. jejuni and 104 c. coli, and 72 were isolated from fowls and swine (16 c. jejuni, 56 c. coli). out of the total of 401 strains, 363 (90.5%) were typable. among human strains, 32 serotypes were identified, more frequently encountered being (in a decreasing order) 29, 4 ...19902101205
management of acute diarrhoea.before 1970, laboratory staff could not only identify the causative organism of acute diarrhea in 20% of cases, but in 1990, they could identify it in 80% of cases. these organisms are either bacteria, virus, or parasites. the bacteria include enterotoxigenic bacteria (vibrio cholerae, enterotoxigenic escherichia coli, clostridium perfringens, and staphylococcus aureus) and enteroinvasive bacteria (campylobacter jejuni, c. coli, and salmonella and shigella species). the leading cause of death ...19902101385
travelers' diarrhea among u.s. navy and marine corps personnel during a western pacific deployment.the incidence and etiology of travelers' diarrhea was studied in a crew of 1,914 sailors and marines aboard a u.s. navy ship during a western pacific deployment. questionnaires completed by 301 troops indicated that 52% had at least one episode of diarrhea during the deployment; however, only 5% of the ship's company sought treatment. enterotoxigenic escherichia coli was the most commonly identified pathogen (23%), followed by giardia lamblia (6%), salmonella (3%), rotavirus (2%), and shigella, ...19902107464
microbiological quality and safety of zoo food.two types of commercial products for feeding zoo animals (a frozen meat product, referred to as zoo food, and a dry product, referred to as dry food) were microbiologically examined for spoilage organisms (aerobic, psychrotrophic, coliform, escherichia coli, mold, and yeasts) and pathogens (salmonella spp., listeria monocytogenes, and campylobacter jejuni). levels of microorganisms in frozen ground zoo food were compared with those in frozen ground beef and frozen ground turkey meat. the level o ...19902111115
acidity and intestinal bacteria: an in-vitro assessment of the bactericidal activity of hydrochloric acid on intestinal pathogens.the bactericidal activity of hydrochloric acid (hcl) on different intestinal bacteria was evaluated at various ph values and time intervals. the effect of hcl was highest at ph 2 and ph 3 on strains of campylobacter jejuni, aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica, salmonella spp. and shigella spp., producing complete killing after 60 min incubation. however, at higher ph values (ph 4 and above), these organisms not only survived but they also showed evidence of ra ...19902127996
[etiological research on acute gastroenteritis in the city of cádiz].we researched the microbial etiology of 5,836 diarrheal processes in outpatients and inpatients at the social security hospital in cádiz, for the purpose of evaluating their microbiological and epidemiological characteristics. bacterial gastroenteritis accounted for 51.6% of the cases, while 31.7% were viral and 16.6% were protozoal in origin. salmonella enterica (35.6%) is the most frequently involved microorganism, especially in the summer, followed very closely by rotavirus (31.7%). the incid ...19902129735
[occurrence of intestinal parasites and campylobacter jejuni in school-age children in krusevac and aleksandrovac]. 19902130015
chicken carcasses as a source of campylobacter jejuni in belo horizonte, brazil.the presence of campylobacter jejuni was investigated in stool specimens from chicken meat workers and in ready-for-market chicken carcasses from one industrial and nine non industrial slaughters in belo horizonte. in the latter c. jejuni was isolated from 19 (38.0%) of the 50 chicken carcasses and from 2 (13.3%) of the stool specimens obtained from 15 chicken meat workers. in the industrial slaughter it was found in only 1 (2.0%) of the 50 chicken carcasses and it was not isolated from any of t ...19902135487
[epidemiology of campylobacter jejuni/coli].the authors describe the epidemic incidence of diseases caused by campylobacter jejuni/coli. during the 5-week follow up period (june 1-july 1, 1988) a total of 74 subjects fell sick. in 31 instances the suspect factor of transmission was non-pasteurized cheese prepared from sheep's milk. in this group of patients a significant shift to higher age categories was noted, contrary to the other 43 diseases. it did not prove possible to isolate campylobacter from cheese, smears from the cottage or fr ...19902139812
campylobacter jejuni-induced vasculitis. 19902151678
[sensitivity of enteropathogenic bacteria to furazolidone].the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ...19902152485
clinical and parasitologic aspects of cryptosporidiosis in nonhuman primates.eighty-one cases of acute cryptosporidiosis were diagnosed among 157 (52%) infant primates, predominantly macaca nemestrina, housed in the nursery unit of the washington regional primate research center. the mean age at onset of oocyst passage was 38 +/- 25 days. the outbreak was confined to the nursery and no cases were detected among juvenile or adult primates housed in other rooms within the colony. all but one animal manifested symptoms of enteric infection, including severe diarrhea and deh ...19902153858
effect of enteroviruses on adherence to and invasion of hep-2 cells by campylobacter isolates.coinfection of hep-2 epithelial cells with coxsackievirus b3, echovirus 7, poliovirus (lsc type 1), porcine enterovirus, and campylobacter isolates was performed to determine if a synergistic effect could be obtained. the invasiveness of campylobacter jejuni atcc 33560 was significantly increased for hep-2 cells preinfected with echovirus 7, coxsackievirus b3, and uv-inactivated (noninfectious) coxsackievirus b3 particles. additionally, the invasiveness of c. jejuni m96, a clinical isolate, was ...19902156779
risk of diarrhea during the first year of life associated with initial and subsequent colonization by specific enteropathogens.the incidence of colonization by enteropathogenic, enterotoxigenic, enteroinvasive, and enterohemorrhagic escherichia coli (detected by dna hybridization with specific radiolabeled probes), salmonella sp., shigella sp., campylobacter jejuni, and rotavirus was related to the presence of diarrhea in a cohort of 75 rural infants followed longitudinally during the first year of life. the study was carried out between august 1985 and february 1987 in the village of lugar sobre la tierra blanca, in th ...19902157338
identification of campylobacter jejuni surface proteins that bind to eucaryotic cells in vitro.to understand the role of campylobacter jejuni surface proteins in the interaction of c. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. this procedure allowed us to antigenically identify c. jejuni outer membrane proteins (omps) that attach to intact host cell membranes. omps isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. however, we found that proteins with molecular masses of 28 and 32 kilodaltons (kda ...19902160431
persistent excretion of rotavirus by pregnant cows.in a study of the epizootiology and prevalence of enteropathogens which may be involved in neonatal calf diarrhoea, 10 in-calf cows from a herd with a history of rotavirus-induced calf diarrhoea were monitored over a period of six to seven months. all the cows excreted rotavirus intermittently without showing any clinical signs, and 21.8 per cent of faecal samples contained rotavirus. reoviruses were isolated from 87 per cent of the samples from the cows, and from all the 10 calves born to them. ...19902164274
emergence of aminoglycoside resistance genes aada and aade in the genus campylobacter.resistance to streptomycin or spectinomycin or both in five campylobacter coli strains, two campylobacter jejuni strains, and a campylobacter-like strain was studied by enzymatic assays and dot blot hybridization. resistance was due to 6- or 3",9-aminoglycoside adenylyltransferases and to new types of phospho- and adenylyltransferases.19902168151
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni ua580.to determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
catalase activity in campylobacter jejuni: comparison of a wild-type strain with an aerotolerant variant.a comparison of campylobacter jejuni vpi strain h840 (atcc 29428), which can grow at o2 levels up to 15%, with variant strain mc711-01 (which can grow at o2 levels up to 21-26%) indicated that the specific activity of catalase in crude cell extracts was higher in the variant by a factor of 1.6 to 2.5, depending on cultural conditions. smaller differences occurred with superoxide dismutase activity, while peroxidase activities were invariably lower in the variant strain. the variant strain was mu ...19902168804
biotypes, serogroups and antibiotic susceptibility of campylobacter jejuni and campylobacter coli in chile.phenotypic markers were studied in 105 strains of thermophilic campylobacters isolated from human beings, animals and drinking water in santiago, chile. the strains were identified as campylobacter jejuni (n = 49) and campylobacter coli (n = 56). biotypes i and ii (lior schema) accounted for 96% c. jejuni isolates, the other 4% being biotype iv but the two biotypes of c. coli were about equally represented. a total of 28 serogroups (lior's heat-labile antigens) were identified. lior 13, 9, 79, 2 ...19902181024
molecular studies on the mechanism of tetracycline resistance mediated by tet(o).the mechanism of resistance to tetracycline in escherichia coli mediated by the campylobacter jejuni-derived resistance determinant tet(o) was investigated. the cloned tet(o) protein had no detectable effect on the intracellular accumulation of tetracycline. the presence of tet(o) markedly diminished the inhibitory effect of tetracycline on protein synthesis both in vivo and in vitro. ribosomes prepared from tetracycline-resistant and susceptible e. coli cells bound almost identical amounts of r ...19902183711
factors responsible for the introduction and spread of campylobacter jejuni infection in commercial poultry production.campylobacter jejuni and related thermophilic campylobacters were not found in a hatchery or in chicks aged less than seven days. however, an increasing proportion of chicks aged two weeks and older shed campylobacters in their droppings. it was shown that a likely source of c jejuni for young chicks was the environment in the immediate vicinity of the rearing houses, and that infection could readily be introduced on the footwear and clothing of farm staff. thermophilic campylobacters were found ...19902188414
to the problem of campylobacter jejuni detectability in water.in a series of model experiments two isolation procedures for the detection of water-borne campylobacter jejuni were compared: a standard culture in thioglycolate broth enriched with 7% defibrinated sheep blood and supplement c and a modified membrane filtration method in which the filter (porosity 0.45 microns) plated on campylobacter agar surface was removed after the first 24 hours of incubation and the plate further incubated for 48 hours. the recovery rates by the thioglycolate broth method ...19902191035
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