| comparison of the substrate kinetics of pig cyp3a29 with pig liver microsomes and human cyp3a4. | cyp (cytochrome p450) 3a29 in pigs could be an important candidate gene responsible for xenobiotic metabolism, similar to cyp3a4 in humans. accordingly, the tissue expression of cyp3a29 mrna in domestic pigs has been determined by a real-time pcr. the enzymatic properties of cyp3a29, cyp3a4 and plm (pig liver microsomes) were compared by kinetic analysis of tst (testosterone) 6β-hydroxylation and nif (nifedipine) oxidation. cyp3a29 mrna was highly expressed in the liver and small intestines of d ... | 2011 | 20863320 |
| distribution of orf2 and orf3 genotypes of porcine circovirus type 2 (pcv-2) in wild boars and domestic pigs in germany. | porcine circovirus 2 (pcv-2), the essential infectious agent in pcvd (porcine circovirus diseases) circulates at high rates among domestic pig and wild boar populations. wild boars may be viremic and shed the virus with excretions and secretions, and thus serve as a reservoir for domestic pig pcv-2 infection. we hypothesize that pcv-2 strains circulating in wild boars and in domestic pigs are significantly different and thus, partially independent. to prove this hypothesis, the present study inv ... | 2010 | 20869816 |
| confirmation of needle cricothyroidotomy using an esophageal detector device in a porcine model. | the difficult intubation is one of the most trying situations encountered in airway management. transtracheal jet ventilation via needle cricothyroidotomy can provide temporary oxygenation in this critical situation in a minimally invasive fashion. the esophageal detector device (edd) has been previously shown to be effective in determining the tracheal placement of endotracheal tubes. in this experiment, three commonly available edds are utilized to accurately identify 15 of 15 intratracheal pl ... | 2010 | 20882932 |
| a novel device for rapid cleaning of poorly prepared colons. | adequate colon preparation is essential for the quality and accuracy of colonoscopy and has a significant influence on related costs. the aim of this study was to assess the efficacy and safety of a novel attachable colon-cleaning device used during colonoscopies in porcine colon. | 2010 | 20886401 |
| the effect of nesting material on the nest-building and maternal behavior of domestic sows and piglet production. | nest building is an important part of maternal behavior in domestic pigs. the aim of the study was to assess the effect of nesting material sawdust vs. straw on sow behavior 24 h before and after birth of the first piglet (bfp) and piglet production. sows, housed in farrowing crates, were randomly divided into 2 treatments: sawdust (n = 12) and straw (n = 13). sawdust and straw were provided during the pre- and parturient period; after parturition, straw was given to both experimental groups. th ... | 2010 | 20889685 |
| meiotic studies in infertile domestic pig-babirusa hybrids. | mating of a babirusa (babyrousa babyrussa) boar and a domestic sow (sus scrofa) resulted in the birth of 5 live domestic pig-babirusa hybrid piglets. chromosome analysis of one of the surviving males confirmed that they were domestic pig-babirusa hybrids by revealing the presence of a complete haploid set of 19 porcine chromosomes as well as a complete haploid set of 19 babirusa chromosomes in the karyotype. none of the surviving piglets, two males and one female, had shown signs of sexual matur ... | 2010 | 20924163 |
| identification of a novel astrovirus in a domestic pig in hungary. | the family astroviridae consists of two genera, avastrovirus and mamastrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. we serendipitously identified a novel porcine astrovirus in a fecal specimen from a domestic pig (sus scrofa domestica) in hungary. sequencing of a fragment indicated that it was an orf1b/orf2/3'utr sequence, and it has been submitted to the database as porcine astrovirus type 2 (pastv-2/hungary/2007) with accession number g ... | 2010 | 20931250 |
| a biochemical comparison of the in vivo effects of bulbine frutescens and bulbine natalensis on cutaneous wound healing. | in south africa the local population relies extensively on indigenous plants in the formulation of traditional medicines to treat skin ailments. the scientific merits of many of these plants used to treat wounds and burns are yet to be validated. bulbine natalensis and bulbine frutescens of the asphodelaceae family are indigenous to only southern africa and are widely used as a skin remedy. this study aimed to explore the scientific value of these plants through investigating the in vivo biochem ... | 2010 | 20937372 |
| next-generation sequencing of the trichinella murrelli mitochondrial genome allows comprehensive comparison of its divergence from the principal agent of human trichinellosis, trichinella spiralis. | the mitochondrial genome's non-recombinant mode of inheritance and relatively rapid rate of evolution has promoted its use as a marker for studying the biogeographic history and evolutionary interrelationships among many metazoan species. a modest portion of the mitochondrial genome has been defined for 12 species and genotypes of parasites in the genus trichinella, but its adequacy in representing the mitochondrial genome as a whole remains unclear, as the complete coding sequence has been char ... | 2010 | 20946970 |
| coronary bare metal stent implantation in homozygous ldl receptor deficient swine induces a neointimal formation pattern similar to humans. | to date, most of all new developments in stent technologies are tested in normal animals. although invaluable in the evaluation of device safety, the juvenile domestic swine (ds) do not follow the biological healing response occurring in humans following coronary stent implantation. by using a novel swine breed afflicted with familial hypercholesterolemia (fhs), we aimed to analyse the vascular response occurring following bare metal stent (bms) implantation by comparing in vivo endovascular ima ... | 2010 | 20950808 |
| magnetically controllable gastrointestinal steering of video capsules. | wireless capsule endoscopy (wce) allows for comfortable video explorations of the gastrointestinal (gi) tract, with special indication for the small bowel. in the other segments of the gi tract also accessible to probe gastroscopy and colonscopy, wce still exhibits poorer diagnostic efficacy. its main drawback is the impossibility of controlling the capsule movement, which is randomly driven by peristalsis and gravity. to solve this problem, magnetic maneuvering has recently become a thrust rese ... | 2010 | 20952324 |
| ochrobactrum pecoris sp. nov., isolated from farm animals. | two gram-negative, rod-shaped, non-spore-forming strains, 08rb2639(t) and 08rb2781-1 isolated from sheep (ovis aries) and boar (sus scrofa domestica), respectively, were studied for their taxonomic position. by 16s rrna gene sequencing, these strains revealed identical sequences and were shown to belong to the alphaproteobacteria with 97.8 % sequence similarity to ochrobactrum rhizosphaerae pr17(t), ochrobactrum pituitosum ccug 50899(t) , ochrobactrum tritici scii24(t) and ochrobactrum haematoph ... | 2010 | 20952542 |
| an elisa based on a truncated soluble orf2 protein for the detection of pcv2 antibodies in domestic pigs. | postweaning multisystemic wasting syndrome (pmws) is an important swine disease that is closely associated with porcine circovirus type 2 (pcv2). the capsid protein (cap protein) is a major structural protein that has at least three immunoreactive regions, and it can be a suitable candidate antigen for detecting the specific antibodies of a pcv2 infection. in the present study, an indirect enzyme-linked immunosorbent assay (tcelisa) based on a truncated soluble cap protein produced in escherichi ... | 2010 | 20960293 |
| left ventricular pacing lead insertion via the coronary sinus cardioplegia cannula: a novel method for temporary biventricular pacing during reoperative cardiac surgery. | objective: temporary biventricular pacing to treat low output states after cardiac surgery is an active area of investigation. reoperative cases are not studied due to adhesions, which preclude left ventricular mobilization to place epicardial pacing wires. in such patients, inserting a temporary left ventricular lead via the coronary sinus cardioplegia cannula may allow for biventricular pacing. we developed a novel technique for intraoperative left ventricular lead placement. methods: eight do ... | 2010 | 20965517 |
| african swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site. | the role of the ancestral sylvatic cycle of the african swine fever virus (asfv) is not well understood in the endemic areas of eastern africa. we therefore analysed the asf infection status on samples collected from 51 free-ranging warthogs (phacocherus africanus) and 1576 ornithodorus porcinus ticks from 26 independent warthog burrows at a single ranch in kenya. abattoir samples from 83 domestic pigs without clinical symptoms, originating from specific locations with no recent reported asf out ... | 2010 | 20965989 |
| [changes in myocardial connexin 43 during ventricular fibrillation]. | to observe changes in connexin 43 (cx43) after ventricular fibrillation (vf) and the effects of rotigaptide (zp123) on cx43. | 2010 | 20977841 |
| a 6-bp deletion in the tyrp1 gene causes the brown colouration phenotype in chinese indigenous pigs. | brown coat colour has been described in chinese-tibetan, kele, and dahe pigs. here, we report the identification of a causal mutation underlying the brown colouration. we performed a genome-wide association study (gwas) on tibetan and kele pigs, and found that brown colours in chinese breeds are controlled by a single locus on pig chromosome 1. by using a haplotype-sharing analysis, we refined the critical region to a 1.5-mb interval that encompasses only one pigmentation gene: tyrosinase-relate ... | 2010 | 20978532 |
| virulence genes and plasmid profiles in rhodococcus equi isolates from domestic pigs and wild boars (sus scrofa) in brazil. | the virulence genes and plasmid profiles of 23 rhodococcus equi isolates from 258 lymph nodes from domestic pigs (129 nodes with lesions and 129 without lesions) and 120 lymph nodes from slaughtered wild boars (60 nodes with lesions and 60 without) were characterized. r. equi was obtained from 19 lymph nodes of domestic pigs, 17 with, and two without lesions, and from four lymph nodes with lesions, from wild boars. the 23 isolates were tested for the presence of vapa and vapb genes, responsible ... | 2010 | 20980033 |
| novel robotic da vinci instruments for laparoendoscopic single-site surgery. | to describe novel robotic laparoendoscopic single-site surgery (r-less) instruments, and present the initial laboratory experience in urology. | 2010 | 20980046 |
| comparative study of the osseointegration of dental implants after different bone augmentation techniques: vascularized femur flap, non-vascularized femur graft and mandibular bone graft. | objectives: the purpose of this study was to evaluate the osseointegration of the dental implants placed into the mandible augmented with different techniques in pigs. material and methods: four adult domestic pigs were used. horizontal augmentation of the mandible was performed in animals by using vascularized femur flap (vff), non-vascularized femur graft (nvfg) and monocortical mandibular block graft (mg). after 5 months of healing 10 dental implants were placed into each augmented site. the ... | 2010 | 21044163 |
| nucleotide variability of the porcine serpina6 gene and the origin of a putative causal mutation associated with meat quality. | the serpin peptidase inhibitor, clade a, member 6 gene (serpina6), also known as corticosteroid-binding globulin or cbg, is involved in obesity and stress sensitivity. previous studies have reported putative causal mutations within that gene in the porcine species. to characterize a hypothetical selective footprint, we have resequenced approximately 6 kb of coding and non-coding fragments in 20 pigs comprising domestic breeds and wild boars from asia and europe. nucleotide variability was found ... | 2010 | 21054451 |
| hybrid transureteral natural orifice translumenal endoscopic nephrectomy: a feasibility study in the porcine model. | natural orifice approaches for nephrectomy have included access via the stomach, vagina, bladder, and rectum. the use of the ureter as a natural orifice for natural orifice translumenal endoscopic surgery (notes) nephrectomy has not been previously reported. the purpose of this study is to test the feasibility of transureteral laparoscopic notes nephrectomy. | 2010 | 21058889 |
| noninvasive mr characterization of structural and functional components of reperfused infarct. | left ventricular (lv) remodeling is a highly complex phenomenon that starts soon after infarction and progresses to extensive regional and global architectural changes over time. | 2010 | 21062130 |
| genetic variations and sequences analysis of mtatp6 and mtatp8 genes among different chinese pig breeds. | to investigate the genetic variations of mtatp6 and mtatp8 genes among different chinese pig breeds, two fragments of 425 and 743 bp containing the whole coding region of mtatp8 and mtatp6 genes were amplified with 805 individuals from 23 chinese local pig breeds, three types of chinese wild boars and three european pig breeds. sequence comparison identified a total number of 17 substitutions including six variable sites in mtatp8 and eleven substitutions in mtatp6 gene. the restriction enzyme f ... | 2010 | 21077971 |
| prevalence of porcine circoviruses in transylvanian wild boars, detected by real-time pcr--short communication. | porcine circoviruses (pcv) are widespread in domestic pigs worldwide and there is growing information about the presence of pcv in other suid species. based on serological studies with sera of wild boars, it was established that pcv1 was present in these animals and antibodies specific to pcv2 were also detected in wild boars living in captivity or in sylvatic areas, both with or without clinical signs of pmws. studies including pcv2 genome or antigen detection confirmed the previous findings. t ... | 2010 | 21087917 |
| mechanical chest compressions with trapezoidal waveform improve haemodynamics during cardiac arrest. | during manual chest compressions for cardiac arrest the waveforms of chest compressions are generally sinusoidal, whereas mechanical chest compression devices can have different waveforms, including trapezoidal. we studied the haemodynamic differences of such waveforms in a porcine model of cardiac arrest. | 2010 | 21093141 |
| gene expression of serotonin and dopamine receptors and monoamine oxidase-a in the brain of dominant and subordinate pubertal domestic pigs (sus scrofa) fed a β-adrenoreceptor agonist. | aggression is a major source of social stress with negative effects on health and well-being, yet limited information is known about the molecular mechanisms mediating aggressive behavior in swine. ractopamine (rac) is a β-adrenoreceptor agonist that enhances growth but increases aggressive behaviors in female pigs. thus, the effects of rac, sex, and social rank on the mrna abundance of genes encoding serotonin and dopamine receptors, and monoamine oxidase (mao)-a in brains of sub-adult pigs wer ... | 2010 | 21094150 |
| an on-farm survey of spatial and temporal stratifications of 17β-estradiol concentrations. | 17β-estradiol (e2) is a natural estrogenic hormone found in animal manure and urine, which may cause endocrine disruption in sensitive organisms. 17β-estradiol has been widely detected in the environment, and animal agriculture may be an important source. the objectives of this study were to investigate the potential sources and/or spatial and temporal characteristics contributing to detections of e2 at the farm-scale. soil cores, segmented into 0.15m increments, were taken down to the water tab ... | 2010 | 21094971 |
| vertebral column, rib and sternal muscles of sulawesi babirusa (babyrousa celebensis). | the muscular anatomy of the vertebrae, ribs and sternebrae of the sulawesi babirusa (babyrousa celebensis) is described. there are many similarities to the anatomy of the domestic pig (sus scrofa). however, unlike other pigs, the m. spinalis et semispinalis cervicis et thoracis has an origin from the twelfth thoracic mamillary process. similarly, the babyrousa does not have the lumbar part of the m. obliquus internus abdominis which is found in other pigs. the m. sacrocaudalis dorsalis medialis ... | 2010 | 21108656 |
| first detection of african swine fever virus in ornithodoros porcinus in madagascar and new insights into tick distribution and taxonomy. | abstract: | 2010 | 21118485 |
| oct4-enhanced green fluorescent protein transgenic pigs: a new large animal model for reprogramming studies. | the domesticated pig has emerged as an important tool for development of surgical techniques, advancement of xenotransplantation, creation of important disease models, and preclinical testing of novel cell therapies. however, germ line-competent pluripotent porcine stem cells have not yet been derived. this has been a major obstacle to genetic modification of pigs. the transcription factor oct4 is essential for the maintenance of pluripotency and for reprogramming somatic cells to a pluripotent ... | 2011 | 21126163 |
| feasibility and safety of endoscopic transesophageal access and closure using a maryland dissector and a self-expanding metal stent. | background: secure access and reliable closure is paramount in the setting of transesophageal mediastinal endoscopic surgery. the purpose of this study was to develop a secure transesophageal access technique and to evaluate the feasibility, safety, and efficacy of a novel covered, self-expanding, retractable stent for closure of 15-mm esophageal defects. methods: fifteen-millimeter esophagotomies were created in 18 domestic pigs using needle knife puncture and balloon dilatation or a blunt diss ... | 2010 | 21136086 |
| a preclinical in vivo investigation of high-intensity focused ultrasound combined with radiotherapy. | this study aims to perform an in vivo investigation evaluating the injury to the pancreas and adjacent tissue of swine resulting with high-intensity focused ultrasound (hifu) combined with radiotherapy (rt). the protocol was approved by the animal ethics committee at the peking university first hospital. a total of 12 domestic swine were divided into four groups: control, hifu only, rt only and hifu + rt. the injury to the pancreas, adjacent tissue and tissue within the acoustic path of the hifu ... | 2011 | 21144959 |
| characterization of plasma triiodophenol binding proteins in vertebrates and tissue distribution of triiodophenol in rana catesbeiana tadpoles. | we investigated the interaction of 2,4,6-triiodophenol (tip), a potent thyroid hormone disrupting chemical, with serum proteins from rainbow trout (onchorhynchus mykiss), bullfrog (rana catesbeiana), chicken (gallus gallus), pig (sus scrofa domesticus), and rat (rattus norvegicus) using a [(125)i]tip binding assay, gel filtration chromatography, and native polyacrylamide gel electrophoresis. [(125)i]tip bound non-specifically to proteins in trout serum, specifically but weakly to proteins in bul ... | 2010 | 21147258 |
| comparison of purified olive oil and silver sulfadiazine in the treatment of partial thickness porcine burns. | introduction: burns are widespread in the developed world, and expensive burn dressings are not universally available. most burn patients suffer from a partial thickness burn that can be treated conservatively. nevertheless, the ideal dressing for the burn wound has not been identified. we performed an animal experiment to compare the healing of partial thickness burns treated with silver sulfadiazine (ssd) and olive oil. methods: a randomized controlled animal experiment was conducted on 3 anes ... | 2010 | 21159471 |
| prins detection of 18s rdna in pig, red fox and chinese raccoon dog, and centromere dna in horse. | the fluorescence in situ hybridization (fish) technique is widely used in animal cytogenetics. contrary to fish procedure, primed in situ dna synthesis (prins) does not require the dna probe preparation (design, synthesis, gel purification of pcr products and labeling). the prins method with primers used as 'dna probes' is both pcr-sensitive and allows for chromosomal localization of dna sequences. here, we show the application of prins reaction with one unlabeled oligonucleotide pair to identif ... | 2010 | 21166802 |
| experimental infection of common warthogs (phacochoerus africanus) and bushpigs (potamochoerus larvatus) with classical swine fever virus ii: a comparative histopathological study. | wild african suidae, the common warthog (phacochoerus africanus) and bushpig (potamochoerus larvatus), were experimentally infected with classical swine fever (csf) virus following the diagnosis of csf subtype 2.1 in domestic pigs in south africa in 2005. no data regarding the susceptibility or potential lesions of these african wild suids are available. seven subadult warthogs and six bushpigs were captured and infected intranasally with the south african isolate. two in-contact control animals ... | 2010 | 21176120 |
| effects of human perturbation on the genetic make-up of an island population: the case of the sardinian wild boar. | game species are often manipulated by human beings, whose activities can deeply affect their genetic make-up and population structure. we focused on a geographically isolated wild boar population (sardinia, italy), which is classified, together with the corsican population, as a separate subspecies (sus scrofa meridionalis). two hundred and ten wild boars collected across sardinia were analysed with a set of 10 microsatellites and compared with 296 reference genotypes from continental wild popul ... | 2010 | 21179064 |
| effects of an alpha-4 integrin inhibitor on restenosis in a new porcine model combining endothelial denudation and stent placement. | restenosis remains the main complication of balloon angioplasty and/or stent implantation. preclinical testing of new pharmacologic agents preventing restenosis largely rely on porcine models, where restenosis is assessed after endothelial abrasion of the arterial wall or stent implantation. we combined endothelial cell denudation and implantation of stents to develop a new clinically relevant porcine model of restenosis, and used this model to determine the effects of an α4 integrin inhibitor, ... | 2010 | 21179441 |
| prevalence and genotypes of toxoplasma gondii in feline faeces (oocysts) and meat from sheep, cattle and pigs in switzerland. | the protozoan parasite toxoplasma gondii infects almost all warm blooded animal species including humans, and is one of the most prevalent zoonotic parasites worldwide. post-natal infection in humans is acquired through oral uptake of sporulated t. gondii oocysts or by ingestion of parasite tissue cysts upon consumption of raw or undercooked meat. this study was undertaken to determine the prevalence of oocyst-shedding by cats and to assess the level of infection with t. gondii in meat-producing ... | 2010 | 21183278 |
| irreversible electroporation in a swine lung model. | this study was designed to evaluate the safety and tissue effects of ire in a swine lung model. | 2010 | 21191587 |
| single-port laparoscopic cholecystectomy with the x-cone: a feasibility study in 9 pigs. | virtually any port system for single-port laparoscopic surgery is for single usage only. the aim of this study was to trial the novel and completely reusable port x-cone in order to perform a cholecystectomy by one infraumbilical incision. | 2010 | 21193478 |
| scanning electron microscopy analysis of luminal inflammation induced by different types of coronary stent in an animal model. | there is histological evidence that drug-eluting stents are associated with delayed endothelialization and a persistent inflammatory state. moreover, clusters of inflammatory cells have been observed on luminal surfaces by scanning electron microscopy. with the aim of quantifying this inflammatory response, we implanted one bare-metal stent and two drug-eluting stents containing different doses of vinblastine embedded in the same polymer into the coronary arteries of 12 domestic pigs. the densit ... | 2010 | 21195533 |
| infrared skin damage thresholds from 1940-nm continuous-wave laser exposures. | a series of experiments are conducted in vivo using yucatan mini-pigs (sus scrofa domestica) to determine thermal damage thresholds to the skin from 1940-nm continuous-wave thulium fiber laser irradiation. experiments employ exposure durations from 10 ms to 10 s and beam diameters of approximately 4.8 to 18 mm. thermal imagery data provide a time-dependent surface temperature response from the laser. a damage endpoint of minimally visible effect is employed to determine threshold for damage at 1 ... | 2010 | 21198172 |
| comparison of electrocardiographic recordings in open-chest and closed-chest swine models. | the aim of our study was to compare the electrocardiographic recordings in an experimental open-chest swine model before and after left-sided thoracotomy to detect any surgery-induced fluctuations that might interfere with subsequent experimental interventions. we obtained electrocardiograms from 8 deeply anesthetized domestic swine and compared the respective st-segment potentials obtained after vascular surgery and after left-sided thoracotomy and dissection of the left anterior descending cor ... | 2010 | 21205452 |
| genetic characterization of african swine fever viruses from a 2008 outbreak in tanzania. | outbreaks of african swine fever (asf) have been reported in the past from several countries in sub-saharan africa. the aim of this study was to genotype asf viruses (asfvs) from the 2008 outbreak in morogoro and dar es salaam regions of tanzania. tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with pcr and genome sequencing methods using asfv-specific primer sets. nucleotide sequence data were obtained for the b646l (p72), e183l ... | 2010 | 21214868 |
| cloning, characterization and diagnostic performance of the salivary lipocalin protein tsgp1 from ornithodoros moubata. | the argasid tick ornithodoros moubata is distributed throughout south and east africa and madagascar, where it colonizes wild and domestic habitats and feeds on warthogs, domestic swine, and humans. this argasid transmits the spirochete borrelia duttonii, causing east african tick-borne relapsing fever in humans, and the african swine fever virus, which causes a highly lethal haemorrhagic disease in pigs. tick surveillance and the elimination of o. moubata from synanthropic environments (human d ... | 2010 | 21216104 |
| suture-free anastomosis of the colon. experimental comparison of two cyanoacrylate adhesives. | we explored the potential of two cyanoacrylate tissue adhesives for constructing colonic anastomoses. | 2011 | 21229329 |
| identification of a second gene associated with variation in vertebral number in domestic pigs. | the number of vertebrae in pigs varies and is associated with body size. wild boars have 19 vertebrae, but european commercial breeds for pork production have 20 to 23 vertebrae. we previously identified two quantitative trait loci (qtls) for number of vertebrae on sus scrofa chromosomes (ssc) 1 and 7, and reported that an orphan nuclear receptor, nr6a1, was located at the qtl on ssc1. at the nr6a1 locus, wild boars and asian local breed pigs had the wild-type allele and european commercial-bree ... | 2011 | 21232157 |
| microrna expression profiling of the porcine developing brain. | micrornas are small, non-coding rna molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. in particular, the developing brain contains an impressive diversity of micrornas. most microrna expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. the domestic pig is considered to be an excellent, alternate, large ... | 2011 | 21253018 |
| distinctive differences in long terminal repeat sequences between γ1 endogenous retroviruses of african and eurasian suid species. | diversity of long terminal repeats (ltrs) from γ1 endogenous retroviruses (ervs) was analysed by dna sequencing in 10 species of the family suidae (suids, pigs and hogs). phylogenetic analysis separated ltr sequences into two groups which correlated approximately with either the previously described cluster i and iii, or the clusters ii, iv and v. interestingly, a specific ltr exhibiting a novel molecular rearrangement was identified exclusively within african host species when compared to ltrs ... | 2011 | 21256982 |
| shen-fu injection attenuates postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. | to investigate the effect of shen-fu injection (sfi) for the management of postresuscitation myocardial dysfunction in a porcine model of cardiac arrest. ventricular fibrillation was induced electrically in anesthetized domestic swine. after 4 min of untreated ventricular fibrillation, cardiopulmonary resuscitation was initiated. sixteen successfully resuscitated pigs were randomized to receive a continuous infusion of either sfi (0.24 mg/min) or saline placebo for 6 h, beginning 15 min after re ... | 2011 | 21263380 |
| differential mrna expression of genes in the porcine adrenal gland associated with psychosocial stress. | to gain insight into the adrenal stress response, we analysed differential mrna expression of genes associated with psychosocial stress in the pig (sus scrofa domestica). various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. we selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. to identify differentially expressed genes, we ... | 2011 | 21266515 |
| application of gis in epizootiological surveillance of swine trichinellosis in one endemic district in serbia. | application of new tools for epizootiological investigations in veterinary medicine, such as geographical information systems (gis), offers a new approach and possibilities for the eradication or control of infectious diseases. gis is particularly useful for research conducted in small areas strongly impacted by man. trichinellosis is a world-wide zoonosis, which is endemic in some european countries, balkan district and serbia in particular. there are very few data on gis application in the fie ... | 2010 | 21275245 |
| identification of low and high frequency ranges for heart rate variability and blood pressure variability analyses using pharmacological autonomic blockade with atropine and propranolol in swine. | understanding autonomic nervous system functioning, which mediates behavioral and physiological responses to stress, offers great potential for assessing farm animal stress and welfare. evaluation of heart rate variability (hrv) and blood pressure variability (bpv), using time and frequency domain analyses may provide a sensitive and reliable measure of affective states and stress-mediated changes in sympathetic and parasympathetic tones. the aim of this research was to define low (lf) and high ... | 2011 | 21281655 |
| molecular mechanism by which amp-activated protein kinase activation promotes glycogen accumulation in muscle. | during energy stress, amp-activated protein kinase (ampk) promotes glucose transport and glycolysis for atp production, while it is thought to inhibit anabolic glycogen synthesis by suppressing the activity of glycogen synthase (gs) to maintain the energy balance in muscle. paradoxically, chronic activation of ampk causes an increase in glycogen accumulation in skeletal and cardiac muscles, which in some cases is associated with cardiac dysfunction. the aim of this study was to elucidate the mol ... | 2011 | 21282366 |
| coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic cardiomyopathy via enhanced neovascularization. | few reports have examined the effects of adult bone marrow multipotent stromal cells (mscs) on large animals, and no useful method has been established for msc implantation. in this study, we investigate the effects of msc infusion from the coronary vein in a swine model of chronic myocardial infarction (mi). mi was induced in domestic swine by placing beads in the left coronary artery. bone marrow cells were aspirated and then cultured to isolate the mscs. at 4 weeks after mi, mscs labeled with ... | 2011 | 21283079 |
| discovery of porcine micrornas in multiple tissues by a solexa deep sequencing approach. | the domestic pig (sus scrofa) is an important economic animal for meat production and as a suitable model organism for comparative genomics and biomedical studies. in an effort to gain further identification of mirnas in the pig, we have applied the illumina solexa sequencing technology to carry out an in-depth analysis of the mirna transcriptome in a pool of equal amounts of rna from 16 different porcine tissues. from this data set, we identified 437 conserved and 86 candidate novel mirna/mirna ... | 2011 | 21283541 |
| the parameters of the porcine eyeball. | the eye of the domestic pig (sus scrofa domestica) is an ex vivo animal model often used in vision sciences research (retina studies, glaucoma, cataracts, etc.). however, only a few papers have compiled pig eye anatomical descriptions. the purpose of this paper is to describe pig and human eye anatomical parameters to help investigators in their choice of animal model depending on their study objective. | 2011 | 21287191 |
| different enteropathogenic yersinia strains found in wild boars and domestic pigs. | yersinia enterocolitica and yersinia pseudotuberculosis strains isolated from wild boars and fattening pigs were characterized and compared with each other. in wild boars, ail-positive y. enterocolitica strains belonged to bioserotypes 4/o:3 (36%, 5/14), 2/o:9 (29%, 4/14), and 2/o:5,27 (21%, 3/14). additionally, two ail-positive strains were untypable. among fattening pigs, the bioserotype 4/o:3 was dominating (91%, 71/78), and bioserotypes 2/o:5,27 (8%, 6/78) and 2/o:9 (1%, 1/78) were rare. inv ... | 2011 | 21288132 |
| trpml2 and the evolution of mucolipins. | trpml2, the polypeptide product of the gene trpml2 (aka mcoln2), is a member of the trpml or mucolipin branch of the trp super family of ion channels. although no known agonists have been discovered, the wild type channel gives basal currents when heterologously expressed in drosophila (s2) cells and is constitutively active in mammalian cells when bearing a cell degeneration-causing, proline to alanine substitution in the fifth trans-membrane domain. trpml2 forms channels that are inwardly rect ... | 2011 | 21290298 |
| changes of dna methylation level and spatial arrangement of primordial germ cells in embryonic day 15 to embryonic day 28 pig embryos. | the mammalian germline is generally assumed to undergo extensive epigenetic reprogramming during embryonic development, including a nearly complete erasure of dna methylation. this assumption does, however, to large degree rely on data from mouse, and despite a well-grounded picture the general nature of these data needs to be validated by investigations of other mammalian species. this study represents such a contribution in the examination of the germline in the domestic pig (sus scrofa). semi ... | 2011 | 21293033 |
| biomechanical testing of spinal segment fixed by thoracolumbar spine locking plate on the swine lumbar spine. | the aim of the experiment was to compare the mechanical properties of intact spinal segment with impaired intervertebral disc and impaired intervertebral disc fixed by tslp (thoracolumbar spine locking plate). | 2010 | 21293547 |
| experimental infection of common warthogs (phacochoerus africanus) and bushpigs (potamochoerus larvatus) with classical swine fever virus. i: susceptibility and transmission. | an incursion of classical swine fever virus (csfv) into the domestic pig population in south africa, identified in 2005, raised the concern that infection might spread to wildlife species and be maintained in these hosts. this study sought to determine whether two wildlife suidae species present in south africa, the bushpig (potamochoerus larvatus) and the common warthog (phacochoerus africanus), could support productive csfv infection. both species could be infected with csfv and transmitted in ... | 2011 | 21294855 |
| the role of movement restrictions and pre-emptive destruction in the emergency control strategy against csf outbreaks in domestic pigs. | classical swine fever (csf) outbreaks in domestic pig herds lead to the implementation of standard control measures according to legislative regulations. ideal outbreak control entails the swift and efficient culling of all pigs on premises detected positive for csf virus. often all pig holdings around the detected cases are pre-emptively destroyed to exclude transmission into the neighbourhood. in addition to these measures, zones are defined in which surveillance and protection measures are in ... | 2011 | 21300412 |
| optical nerve detection by diffuse reflectance spectroscopy for feedback controlled oral and maxillofacial laser surgery. | laser surgery lacks haptic feedback, which is accompanied by the risk of iatrogenic nerve damage. it was the aim of this study to investigate diffuse reflectance spectroscopy for tissue differentiation as the base of a feedback control system to enhance nerve preservation in oral and maxillofacial laser surgery. | 2011 | 21310023 |
| neuronal genes for subcutaneous fat thickness in human and pig are identified by local genomic sequencing and combined snp association study. | obesity represents a major global public health problem that increases the risk for cardiovascular or metabolic disease. the pigs represent an exceptional biomedical model related to energy metabolism and obesity in humans. to pinpoint causal genetic factors for a common form of obesity, we conducted local genomic de novo sequencing, 18.2 mb, of a porcine qtl region affecting fatness traits, and carried out snp association studies for backfat thickness and intramuscular fat content in pigs. in o ... | 2011 | 21311593 |
| combining information from surveys of several species to estimate the probability of freedom from echinococcus multilocularis in sweden, finland and mainland norway. | the fox tapeworm echinococcus multilocularis has foxes and other canids as definitive host and rodents as intermediate hosts. however, most mammals can be accidental intermediate hosts and the larval stage may cause serious disease in humans. the parasite has never been detected in sweden, finland and mainland norway. all three countries require currently an anthelminthic treatment for dogs and cats prior to entry in order to prevent introduction of the parasite. documentation of freedom from e. ... | 2011 | 21314948 |
| assessment of interactions between african swine fever virus, bushpigs (potamochoerus larvatus), ornithodoros ticks and domestic pigs in north-western madagascar. | since its introduction in madagascar in 1998, african swine fever (asf) has severely affected national pig production and persists as a common disease in that country. two of its natural hosts in the african continent, the bushpig (potamochoerus larvatus) and tick vectors of the ornithodoros moubata complex, are reported in west and central regions of the island. however, their role in the maintenance and transmission of the virus has been insufficiently studied. in this work, we tried to assess ... | 2011 | 21320295 |
| a long-term serological survey on aujeszky's disease virus infections in wild boar in east germany. | summarybetween 1985 and 2008, a total of 102 387 wild boar sera originating from eastern germany covering an area of 108 589 km2 were tested for the presence of aujeszky's disease virus (adv)-specific antibodies. from 1985 until 1991 and from 1992 until 2008, wild boar sera were exclusively investigated using either conventional seroneutralization assays (n=39 621) or commercial gb and full antigen elisas (n=62 766), respectively. spatial-temporal analysis revealed an increasing adv seroprevalen ... | 2011 | 21320372 |
| discovery of hepatitis e: the epidemic non-a, non-b hepatitis 30 years down the memory lane. | hepatitis e was first recognised during an epidemic of hepatitis, which occurred in kashmir valley in 1978. the epidemic involved an estimated 52,000 cases of icteric hepatitis with 1700 deaths. the disease had unique clinical and epidemiological features. the epidemic was water-borne with highly compressed epidemic curve. following the epidemic, secondary waves of hepatitis did not occur. clinical profile was characterized by cholestasis in around 20% of patients. the disease predominantly occu ... | 2011 | 21320558 |
| markers of progenitor cell recruitment and differentiation rise early during ischemia and continue during resuscitation in a porcine acute ischemia model. | clinical administration of bone marrow-derived stem cells in the setting of acute myocardial infarction (ami) leads to improved left ventricular ejection fraction. thymosin beta-4 (tb4) and vascular endothelial growth factor (vegf) are linked to adult epicardial progenitor cell mobilization and neovascularization and is cardioprotective after myocardial ischemia. this study investigated the time course of tb4 and vegf during ami, cardiac arrest, and resuscitation. fifteen anesthetized and instru ... | 2011 | 21332365 |
| vascular access port implantation and serial blood sampling in a gottingen minipig (sus scrofa domestica) model of acute radiation injury. | threats of nuclear and other radiologic exposures have been increasing, but no countermeasure for acute radiation syndrome has been approved by regulatory authorities. because of their similarity to humans in regard to physiology and anatomy, we are characterizing gottingen minipigs as a model to aid the development of radiation countermeasures. irradiated minipigs exhibit immunosuppression, severe thrombocytopenia, vascular leakage, and acute inflammation. these complications render serial acqu ... | 2011 | 21333166 |
| microsurgical cerebral aneurysm training porcine model. | the authors developed a simple reproducible technique for aneurysm creation and adapted it to mimic intracranial dissection conditions using glue application as a pseudo-arachnoid type layer. ten 1-2-month-old healthy domestic swine were employed under general anesthesia. a novel technique for bifurcation aneurysm creation was developed using two arteries and a vein. after aneurysm creation, diluted sulfuric acid was applied on the dome with a micropipette to increase aneurysm fragility in selec ... | 2011 | 21339668 |
| epigenetic reprogramming in the porcine germ line. | epigenetic reprogramming is critical for genome regulation during germ line development. genome-wide demethylation in mouse primordial germ cells (pgc) is a unique reprogramming event essential for erasing epigenetic memory and preventing the transmission of epimutations to the next generation. in addition to dna demethylation, pgc are subject to a major reprogramming of histone marks, and many of these changes are concurrent with a cell cycle arrest in the g2 phase. there is limited information ... | 2011 | 21352525 |
| sodium nitroprusside enhanced cardiopulmonary resuscitation improves survival with good neurological function in a porcine model of prolonged cardiac arrest. | to assess the effectiveness of sodium nitroprusside (snp)-"enhanced" cardiopulmonary resuscitation (snpecpr) on 24-hr survival rates compared to standard cpr in animals after cardiac arrest. snpecpr consists of large intravenous snp bolus doses during cpr enhanced by active compression-decompression cpr, an inspiratory impedance threshold device (itd), and abdominal binding (ab). the combination of active compression-decompression cpr+itd+ab without snp will be called "enhanced" or ecpr. | 2011 | 21358401 |
| nonlinear microscopy, infrared, and raman microspectroscopy for brain tumor analysis. | contemporary brain tumor research focuses on two challenges: first, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. second, for prognostication the tumor has to be removed as completely as possible. nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. however, it is neither applicable in vivo, nor does it allow for precise tumor typing i ... | 2011 | 21361676 |
| the response of cranial biomechanical finite element models to variations in mesh density. | finite element (fe) models provide discrete solutions to continuous problems. therefore, to arrive at the correct solution, it is vital to ensure that fe models contain a sufficient number of elements to fully resolve all the detail encountered in a continuum structure. mesh convergence testing is the process of comparing successively finer meshes to identify the point of diminishing returns; where increasing resolution has marginal effects on results and further detail would become costly and u ... | 2011 | 21370496 |
| distribution of myocardial bridges in domestic pig. | localisation and morphology of myocardial bridges in the heart of domestic pig remain an open issue. since these structures significantly influence haemodynamics in the coronary arteries, their occurance may lead to numerous pathologies. in the examined group of 150 domestic pig's hearts, myocardial bridges were diagnosed in 47.3% of the material, mostly in males. in majority of cases the bridges were present above the posterior interventricular branch of the right coronary artery, less often ab ... | 2010 | 21370748 |
| genetic characterisation of african swine fever viruses from recent and historical outbreaks in sardinia (1978-2009). | three discrete regions of the african swine fever virus (asfv) were analysed in the genomes of a wide range of isolates collected from wild and domestic pigs in sardinia, over a 31-year period (1978-2009). the analysis was conducted by genotyping based on sequence data from three single copy asf genes. the e183l gene encoding the structural protein p54 and part of the gene encoding the p72 protein were used to delineate genotypes, before intra-genotypic resolution of viral relationships by analy ... | 2011 | 21373994 |
| plasma and tissue levels of neuropeptide y in experimental septic shock: relation to hemodynamics, inflammation, oxidative stress, and hemofiltration. | neuropeptide y (npy), a potent vasoconstrictor released from the sympathetic nerves, has been suggested to counterbalance sepsis-induced vasodilation. thus, the changes in plasma and tissue npy concentrations in relation to hemodynamic variables and inflammatory markers in a porcine model of moderate septic shock were investigated. susceptibility of npy to be removed by continuous hemofiltration in two settings has been also studied. thirty-four domestic pigs were divided into five groups: (i) c ... | 2011 | 21375544 |
| developmental changes affecting lectin binding in the vomeronasal organ of domestic pigs, sus scrofa. | this study investigated the developmental changes of glycoconjugate patterns in the porcine vomeronasal organs (vnos) and associated glands (jacobson's glands) from prenatal (9 weeks of gestation) and postnatal (2 days after birth) to the sexually mature stage (6 months old). the vno of pigs (sus scrofa) was examined using the following: dolichos biflorus agglutinin (dba), bandeiraea simplicifolia agglutinin isolectin b4 (bsi-b4), triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i ( ... | 2011 | 21376374 |
| mycobacterium caprae infection in livestock and wildlife, spain. | mycobacterium caprae is a pathogen that can infect animals and humans. to better understand the epidemiology of m. caprae, we spoligotyped 791 animal isolates. results suggest infection is widespread in spain, affecting 6 domestic and wild animal species. the epidemiology is driven by infections in caprids, although the organism has emerged in cattle. | 2011 | 21392452 |
| elevated japanese encephalitis virus activity monitored by domestic sentinel piglets in thailand. | twenty-nine domestic piglets from pig farms located in three provinces of thailand between 2003 and 2004 were used as sentinel animals for japanese encephalitis virus (jev) circulation. piglets were used as sentinel to underline, on one hand, the role of domestic pigs as jev amplifying host and, on another hand, to point out the interest of using sentinel animals for japanese encephalitis surveillance. jev activity was demonstrated through i/ antibody detection using a specific elisa test for th ... | 2011 | 21395418 |
| two cases of reciprocal translocations in domestic pigs producing small litters. | summary: two boars of finnish yorkshire breed were selected for chromosomal analysis because of their small litters. one had a complex three breakpoint reciprocal translocation where the small distal chromosome segments were fused in new combinations between autosomes 2,9 and 14. this boar with a 2n = 38,xy,t(2;9;14)(q23;q22;q25) karyotype had produced approximately 3.3 piglets per litter and its fertility was reduced by 70% compared to average of litter size in finnish yorkshire breed pigs. in ... | 1997 | 21395832 |
| microdialysis assessment of percutaneous penetration of ketoprofen after transdermal administration to hairless rats and domestic pigs. | the study was performed to evaluate the percutaneous penetration of ketoprofen after transdermal administration using a microdialysis technique in pigs, in comparison with rats. ketoprofen release from patches was determined by analysis of the remaining drug content after application to hairless rats and pigs. skin and knee joint penetration of ketoprofen was tested by microdialysis, and recovery was determined by retrodialysis. residual rates in hairless rats and pigs were 68.1 -¦ 1.6% and 81.7 ... | 2011 | 21397690 |
| validation of an automated method for salivary alpha-amylase measurements in pigs (sus scrofa domesticus) and its application as a stress biomarker. | the aim of the current study was to validate an automated spectrophotometric method for salivary alpha-amylase measurement in pigs and evaluate its possible application as a noninvasive stress biomarker. the analytical validation included intra- and interassay precision, linearity under dilution, and limit of detection. in addition, to study the possible use of salivary alpha-amylase as a possible stress marker, 12 crossbred growing pigs of 3-4 months of age were subjected to restraint stress by ... | 2011 | 21398448 |
| intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model. | reperfusion injury (ri) remains an important limitation of myocardial revascularization. the aim of the present study was to evaluate the influence of the intracoronary injection of adiponectin on ri and cardiomyocyte death in a porcine myocardial infarction model. acute infarction in 14 polish domestic pigs was induced by inflation of an over the wire balloon (otw) catheter in the medial left anterior descending artery for 60-ámin. the study group consisted of 7 pigs in which intracoronary adip ... | 2011 | 21399860 |
| second prize: in-vivo physiological impact of alpha blockade on the porcine ureter with distal ureteral obstruction. | alpha blockade has been well described clinically for expulsion of distal ureteral stone. however, the in-vivo effects of a-blockade on ureteral dynamics during stone passage are not clear. we studied the effects of oral alfuzosin on ureteral pressure and peristalsis in a distally obstructed porcine ureter. | 2011 | 21401393 |
| vardenafil effect on ureteric smooth muscle: in vitro study in porcine model. | phosphodiesrase type 5 inhibitors have been recently reported to induce a relaxing effect on ureteral smooth muscle. we conducted an in vitro study to elucidate the relaxing effect of various doses of vardenafil on the porcine ureter. moreover, we propose a porcine ureter model for the evaluation of the effect of different substances on the ureter. | 2011 | 21401398 |
| age-related changes in corticosteroid receptor expression and monoamine neurotransmitter concentrations in various brain regions of postnatal pigs. | negative early life experience may be associated with altered functioning of stress-related systems and may increase vulnerability to diseases later in life. corticosteroids are important mediators of homeostasis and stress and exert their effects via two receptors, the mineralocorticoid receptor (mr) and the glucocorticoid receptor (gr), and through the glucocorticoid-metabolizing enzymes 11ß-hydroxysteroid dehydrogenase (11ß-hsd) types 1 and 2 in a brain-region-specific manner. however, relati ... | 2011 | 21416484 |
| the effect of combined delivery of recombinant human bone morphogenetic protein-2 and recombinant human vascular endothelial growth factor 165 from biomimetic calcium-phosphate-coated implants on osseointegration. | objectives: the delivery of growth factors for enhanced osseointegration depends on the effectiveness of the carrier systems at the bone-implant interface. this study evaluated the effect of solo and dual delivery of recombinant human bone morphogenetic protein-2 (rhbmp-2) and recombinant human vascular endothelial growth factor (rhvegf(165) ) from biomimetically octacalcium phosphate-coated implants on osseointegration. materials and methods: biomimetic implants, bearing either a single growth ... | 2011 | 21418332 |
| foot-and-mouth disease in feral swine: susceptibility and transmission. | experimental studies of foot-and-mouth disease (fmd) in feral swine are limited, and data for clinical manifestations and disease transmissibility are lacking. in this report, feral and domestic swine were experimentally infected with fmdv (a24-cruzeiro), and susceptibility and virus transmission were studied. feral swine were proved to be highly susceptible to a-24 cruzeiro fmd virus by intradermal inoculation and by contact with infected domestic and feral swine. typical clinical signs in fera ... | 2011 | 21418546 |
| welfare assessment in porcine biomedical research - suggestion for an operational tool. | in recent years, increasing interest in using the pig (sus scrofa) for biomedical research has become evident. today, the pig is considered an advantageous alternative animal model for various human diseases and conditions. however, even though a considerable amount of biomedical research has been done on pigs, hardly any studies include systematic welfare assessment. still, it is essential to assess welfare of laboratory pigs, both domestic pig breeds and smaller purpose-bred breeds, as (1) sci ... | 2011 | 21420697 |
| effects of local intracoronary paclitaxel delivery using the remedy transport catheter on neointimal hyperplasia after stent implantation in a porcine model. | to assess the effects of local paclitaxel delivery using the remedy catheter on neointimal hyperplasia in a porcine model and compare these results to commercially available bms and biodegradable polymer-coated paclitaxel-eluting stents (bp-pes). | 2011 | 21421185 |
| prevalence of classical swine fever virus in india: a 6-year study (2004-2010). | a study was undertaken regarding the prevalence of classical swine fever virus (csfv) antibodies and antigens in sera and suspected tissue samples of domestic pigs. the samples were received between january 2004 and september 2010. a total of 594 serum samples from 12 states and 287 tissue samples from 13 states of india were tested using commercial enzyme-linked immunosorbent assay (elisa) kits. the mean prevalence of csfv antibodies in suspected sera was 63.3% (376/594), whereas 76.7% (220/287 ... | 2011 | 21429182 |
| behavioural and cardiac responses towards conspecific distress calls in domestic pigs (sus scrofa). | in domestic pigs, vocalisation can be an indicator of distress and negative emotional states. it might play a role in the transfer of emotion between individuals ('emotional contagion' or 'empathy'), which could result in impaired animal welfare on a group level based on the distress in an individual member of the group. the aim of this study was to characterise the responses of pigs to conspecific distress calls. we performed a playback experiment in an open arena with 24 juvenile german landra ... | 2011 | 21439307 |
| comparative analysis of osseointegration of titanium implants with acid-etched surfaces and different biomolecular coatings. | objectives: an increasing trend toward implantation in complex cases, as well as early loading, is beginning to emerge in dental implantology. long-term stability of the inserted implants greatly depends on the osseointegration process. although there are numerous current research efforts aimed at functionalizing implant surfaces, no single factor has proved to be beneficial for osseointegration. the aim of the present study was to investigate whether a combination coating of collagen i and diff ... | 2011 | 21441047 |
| prevalence of antibody to toxoplasma gondii and trichinella spp. in feral pigs (sus scrofa) of eastern north carolina. | feral pigs (sus scrofa) survive in many climates, reproduce year-round, and are dietary generalists. in the united states, the size and range of the feral pig population has expanded, resulting in greater interaction with humans and domestic swine and increased potential for disease transmission. we conducted a serosurvey in feral pigs from eastern north carolina to determine exposure to the zoonotic parasites, toxoplasma gondii and trichinella spp. between september 2007 and march 2009, blood s ... | 2011 | 21441186 |
| semi-preparative isolation and purification of three tauro-conjugated cholic acids from pulvis fellis suis by hsccc coupled with elsd detection. | coupled with evaporative light scattering detection, a high-speed counter-current chromatography (hsccc) method was applied to the separation and purification of three tauro-conjugated cholic acids of taurochenodeoxycholic acid (tcdca), taurohyodeoxycholic acid (thdca) and taurohyocholic acid (thca) from pulvis fellis suis (pig gallbladder bile) for the first time. the two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v/v/v) was selected for the one-step se ... | 2011 | 21442029 |