| serum tsh and serum thyroid peroxidase antibody fluctuate in parallel and high urinary iodine excretion predicts subsequent thyroid failure in a 1-year study of patients with untreated subclinical hypothyroidism. | to explore the possibility of predicting decline or improvement in thyroid function over 1 year, and to investigate the correlations of serum tsh (s-tsh) with hypothyroidism-related symptoms and signs, serum thyroid peroxidase antibody (s-tpo-ab) and urinary iodine excretion in individual patients with untreated subclinical hypothyroidism (sh). | 2008 | 18230828 |
| influence of probiotic and conventional yoghurt on the status of vitamins b1, b2 and b6 in young healthy women. | in vitro studies indicate that yoghurt bacteria are able to generate several water-soluble vitamins and therefore yoghurt could be a good source of these micronutrients. however, whether lactobacilli or other viable bacteria release the synthesized vitamins or utilize vitamins from their surroundings is a matter of debate. this study was carried out to investigate whether probiotic and traditional yoghurt bacteria are able to influence the status of different b vitamins (b(1), b(2), b(6)) in you ... | 2008 | 18230968 |
| molecular characterization and expression analysis of five different elongation factor 1 alpha genes in the flatfish senegalese sole (solea senegalensis kaup): differential gene expression and thyroid hormones dependence during metamorphosis. | eukaryotic elongation factor 1 alpha (eef1a) is one of the four subunits composing eukaryotic translation elongation factor 1. it catalyzes the binding of aminoacyl-trna to the a-site of the ribosome in a gtp-dependent manner during protein synthesis, although it also seems to play a role in other non-translational processes. currently, little information is still available about its expression profile and regulation during flatfish metamorphosis. with regard to this, senegalese sole (solea sene ... | 2008 | 18234081 |
| the atpase domain of the large terminase protein, gp17, from bacteriophage t4 binds dna: implications to the dna packaging mechanism. | translocation of double-stranded dna into a preformed capsid by tailed bacteriophages is driven by powerful motors assembled at the special portal vertex. the motor is thought to drive processive cycles of dna binding, movement, and release to package the viral genome. in phage t4, there is evidence that the large terminase protein, gene product 17 (gp17), assembles into a multisubunit motor and translocates dna by an inchworm mechanism. gp17 consists of two domains; an n-terminal atpase domain ... | 2008 | 18234214 |
| p16ink4 immunoreactivity is a reliable marker for urothelial carcinoma in situ. | morphological discrimination of urothelial carcinoma (uc) in situ, a precursor of high-grade invasive carcinoma, from severe reactive atypia is essential, but can be challenging at times. mutations of the cell cycle regulatory gene ink4a (located at 9p21) that encodes p16(ink4) are one of the critical early events in carcinogenesis of uc. the purpose of this study was to investigate p16(ink4) expression in different urothelial lesions and assess its possible utility in distinguishing reactive ve ... | 2008 | 18234280 |
| simulation of nitroxide electron paramagnetic resonance spectra from brownian trajectories and molecular dynamics simulations. | a simulated continuous wave electron paramagnetic resonance spectrum of a nitroxide spin label can be obtained from the fourier transform of a free induction decay. it has been previously shown that the free induction decay can be calculated by solving the time-dependent stochastic liouville equation for a set of brownian trajectories defining the rotational dynamics of the label. in this work, a quaternion-based monte carlo algorithm has been developed to generate brownian trajectories describi ... | 2008 | 18234808 |
| phase ii trial of preoperative chemoradiotherapy followed by surgical resection in patients with superior sulcus non-small-cell lung cancers: report of japan clinical oncology group trial 9806. | to evaluate the safety and efficacy of preoperative chemoradiotherapy followed by surgical resection for superior sulcus tumors (ssts). | 2008 | 18235125 |
| [mr-endoscopic us correlation for loco-regional staging of rectal carcinoma]. | to correlate findings at high-resolution mr and endoscopic us (eus) for preoperative loco-regional staging of rectal carcinoma. | 2007 | 18235347 |
| [non-small cell lung cancer : the limits of surgical resection]. | the international staging system for lung cancer actually subdivides patients in two subcategories, according to surgical resectability. theoretically the landmark is between stages iiia and iiib. non-resectability of t4 tumors is controversial. because technical advances in surgical techniques and perioperative care, several selected patients with mediastinal involvement (left atrium, great vessels, carina, spine) can be resected with encouraging long-term outcomes. published data on surgical t ... | 2007 | 18235416 |
| follicular histotypes of oncocytic thyroid carcinomas do not carry mutations of the braf hot-spot. | the braf v600e mutation is the most prevalent genetic aberration in papillary thyroid carcinomas (ptcs), and it is found exclusively in ret/ptc-negative tumors. in oncocytic (hürthle cell, oxyphilic) thyroid tumors, the presence of ret/ptc rearrangements is associated with either the conventional papillary histotype or the "solid" hürthle cell tumors, whereas all predominantly follicular oncocytic carcinomas do not harbor ret/ptc chimeras. although 12% of tumors of the follicular variant of ptc ... | 2008 | 18235983 |
| [determining the thyroid hormones t3 and t4 in the urine: an unreliable test for hypothyroidism]. | a 24-year-old woman complained of tiredness, sensitivity to cold, and feelings of depression. a diagnosis of hypothyroidism based on decreased 24 h urinary t3 and t4 excretion was made, and she was treated with levothyroxin. no blood tests were done. she was referred with the question if she had other endocrine disorders. her periods were regular, and on physical examination no abnormalities except slight acne were found. similarly, hypothyroidism was diagnosed by decreased thyroid hormone excre ... | 2007 | 18237046 |
| association of first-trimester thyroid function test values with thyroperoxidase antibody status, smoking, and multivitamin use. | to determine first-trimester thyroid function values and associations with thyroperoxidase antibody (tpo-ab) status, smoking, emesis, and iodine-containing multivitamin use. | 2008 | 18238739 |
| exacerbation of underlying hypothyroidism caused by proteinuria and induction of urinary thyroxine loss: case report and subsequent investigation. | to describe a patient with excess urinary thyroxine (t4) excretion and worsening of preexisting hypothyroidism in the setting of nephrotic syndrome and to determine whether excess urinary t4 excretion is present in other patients with proteinuria. | 2008 | 18238748 |
| comparison of a new neuromuscular transmission monitor compressomyograph with mechanomyograph. | we developed a new neuromuscular transmission monitor, the compressomyograph (cmg, european patent number: ep 06018557.6, us patent number: us 60/824.541). this is the first preliminary report comparing neuromuscular block monitored by cmg and the relaxometer mechanomyograph (mmg). | 2008 | 18238840 |
| depth of tumor invasion in locally advanced rectal cancer correlates with patients' prognosis: the usefulness of elastic stain for its measurement. | the relationship between the prognosis and the extent of colorectal carcinoma (crc) is still unclear. as a simple parameter of the local invasion of crc, we assessed the extent of tumor invasion beyond the outer border of the muscularis propria (mp). | 2008 | 18239867 |
| compliance with melanoma treatment guidelines in a community teaching hospital: time trends and other variables. | variation in the surgical treatment of melanoma occurs despite efforts to standardize care. this may lead to morbidity, inaccurate staging, and poor outcomes, or it may be cost ineffective. the purpose of our study was to evaluate our institutional compliance with national comprehensive cancer network (nccn) melanoma treatment guidelines. | 2008 | 18239975 |
| 5-ht(7) receptors in the modulation of cognitive processes. | the abundance of serotonin (5-ht) in the central nervous system can explain its role in the regulation of various functions, such as sleep, feeding, sexuality, emotional status, and pain. in addition, 5-ht localized in "cognitive pathways" with hippocampus and frontal cortex as the main target structures, is involved in learning and memory processes. recent studies led to the discovery of various types and subtypes of receptors, differentially associated to cognitive mechanisms. abundant data av ... | 2008 | 18243350 |
| pyruvate kinase isoenzyme m2 is not of diagnostic relevance as a marker for oral cancer. | increased aerobic glycolysis is one of the most common abnormalities occurring in the metabolism of tumour cells with pyruvate kinase as one of the key glycolytic enzymes. the dimeric form of m2-pyruvate kinase, found predominantly in tumour cells, is designated as tumour m2-pyruvate kinase (tum2-pk). the aim of the present, prospective study was to evaluate the diagnostic relevance of tum2-pk in detecting oral squamous cell carcinoma (oscc). | 2008 | 18243718 |
| templated mutagenesis in bacteriophage t4 involving imperfect direct or indirect sequence repeats. | some mutations arise in association with a potential sequence donor that consists of an imperfect direct or reverse repeat. many such mutations are complex; that is, they consist of multiple close sequence changes. current models posit that the primer terminus of a replicating dna molecule dissociates, reanneals with an ectopic template, extends briefly, and then returns to the cognate template, bringing with it a locally different sequence; alternatively, a hairpin structure may form the mutati ... | 2008 | 18245334 |
| development of a multiplex rna urine test for the detection and stratification of transitional cell carcinoma of the bladder. | new markers that enable the percentage of transitional cell carcinomas (tcc) of the bladder that are diagnosed before invasion of the bladder muscle layers to be increased would reduce the morbidity and mortality associated with this disease. the purpose of this study was to develop a simple, accurate urine test based on mrna markers and simple gene signatures that (a) could detect tcc before muscle invasion while maintaining high specificity in patients with hematuria or urinary tract infection ... | 2008 | 18245534 |
| predicting intraoperative vertebral rotation in patients with scoliosis using posterior elements as anatomical landmarks. | a retrospective study. | 2007 | 18245992 |
| anterior approach to the cervicothoracic junction without sternotomy: a report of 37 cases. | description of a new anterior approach to the cervicothoracic junction and retrospective study of results. | 2007 | 18246011 |
| dissecting the matrilineal components of tongjiang cattle from southwest china. | tongjiang cattle are a local cattle population of sichuan province, china, numbering approximately half a million in 2005. they have long been grouped into the bashan breed, although they have a unique breeding history and phenotypic characteristics, as well as a restricted geographic distribution. morphologically, they can be divided into two groups based on the basic coat color (black and russet). in order to dissect the matrilineal components of tongjiang cattle and to compare the body size t ... | 2008 | 18246422 |
| pten expression is a strong predictor of survival in mesothelioma patients. | malignant pleural mesothelioma (mpm) is a highly aggressive tumour with poor prognosis and limited response to therapy. mpm is characterised by complex chromosomal aberrations, including chromosome 10 losses. the tumour suppressor gene phosphatase and tensin homologue deleted from chromosome 10 (pten) located on chromosome 10q23 plays an important role in different cancer, but its relevance for mpm is unclear. | 2008 | 18248818 |
| non-small cell lung cancer with ipsilateral pulmonary metastases: prognosis analysis and staging assessment. | although designated as t4 or m1 in the current tnm classification system revised in 1997, non-small cell lung cancer with ipsilateral pulmonary metastases is treated as a locally advanced disease and reported survival rates are relatively good. we intended to analyze the prognosis of ipsilateral pulmonary metastases and validate current tnm classification system. | 2008 | 18249000 |
| primary tumor volume of nasopharyngeal carcinoma: significance for survival. | to elucidate the effect of primary tumor volume (ptv) on treatment outcomes in patients with nasopharyngeal carcinoma (npc) who were treated with radiotherapy or concurrent chemoradiotherapy. the tnm staging system developed by american joint committee of cancer (ajcc) is universally used and accepted but its prediction of prognosis in npc receives a lot of challenge. primary tumor volume had been reported to have close relationship with prognosis of head and neck cancer. we may predict prognosi ... | 2008 | 18249078 |
| long-term changes of the upper lip position relative to the incisal edge. | one result of postadolescent facial growth is elongation of the upper lip and subsequent decrease of incisal display on smiling. the aim of this study was to evaluate the long-term change of upper lip position relative to the incisal edge. | 2008 | 18249286 |
| iodine status among pregnant women in kuwait. | up to now, little has been known about iodine intake and the prevalence of iodine deficiency (id), if any, in kuwait. urinary iodine excretion (uie) and changes in thyroid function during pregnancy were thus evaluated. | 2007 | 18250611 |
| effects of ghrelin administration on endocrine and metabolic parameters in obese women with polycystic ovary syndrome. | the novel peptide ghrelin displays multiple endocrine and non-endocrine actions. its strong gh-releasing activity in humans has long been recognized. however, in obesity, ghrelin administration induces a blunted gh secretion, enhances glucose and reduces insulin levels. the effects of ghrelin administration have not been investigated in polycystic ovary syndrome (pcos), which can be associated with obesity, hyperinsulinism, and gh hyposecretion. leptin is a mediator for energy balance opposed to ... | 2007 | 18250617 |
| a case of thyrotropin-producing pituitary adenoma, accompanied by an increase in anti-thyrotropin receptor antibody after tumor resection. | we describe a rare, but interesting, case of tsh-producing adenoma (tshoma), accompanied by increases in both anti-tsh receptor antibody (trab) and thyroid-stimulating antibody (tsab) after tumor resection. a 21-yr-old woman was referred to our department for further evaluation of pituitary tumor. in a nearby hospital, she had been diagnosed as having pituitary tumor. her serum free t4, free t3, and tsh levels were all elevated concomitantly. on the basis of a diagnosis of pituitary adenoma with ... | 2007 | 18250618 |
| [endocrinology]. | treatment and follow-up of differenciated thyroid carcinoma and use of recombinant human tsh in the case of ablative radio-iodine treatment or thyroglobulin stimulation are discussed in the context of new international recommandations. subclinical thyroid disease and abnormal thyroid function tests during pregnancy are the other topics. treatment of subclinical hyperthyroidism is recommended when tsh is below 0.1 mu/l because of cardiac and bone complication while a substitutive thyroid hormone ... | 2008 | 18251209 |
| nickel, chromium and iron levels in the saliva of patients with simulated fixed orthodontic appliances. | to assess the in vivo release of nickel, chromium, and iron ions into saliva by different metallic brackets. | 2008 | 18251615 |
| ingestion and inactivation of bacteriophages by tetrahymena. | abiotic factors are thought to be primarily responsible for the loss of bacteriophages from the environment, but ingestion of phages by heterotrophs may also play a role in their elimination. tetrahymena thermophila has been shown to ingest and inactivate bacteriophage t4 in co-incubation experiments. in this study, other tetrahymena species were co-incubated with t4 with similar results. in addition, t. thermophila was shown to inactivate phages t5 and lambda in co-incubations. several approach ... | 2008 | 18251802 |
| transthyretin levels are not related to apgar score in low birth weight and very low birth weight infants. | previous studies have reported an increased incidence of thyroid dysfunction in premature/low birth weight infants. the cord blood concentrations of transthyretin (ttr), a thyroid hormone binding protein, have also been found to be decreased in preterm infants. while thyroid hormone concentrations are decreased in sick infants, it is not known if physical condition influences ttr levels. serial concentrations of ttr following birth have not previously been reported. | 2008 | 18252270 |
| endocrine effects of tetrabromobisphenol-a (tbbpa) in wistar rats as tested in a one-generation reproduction study and a subacute toxicity study. | endocrine effects of the brominated flame retardant tetrabromobisphenol-a (tbbpa) were studied in a one-generation reproduction assay in wistar rats via repeated dietary exposure, applying eight dose groups at 0-3-10-30-100-300-1,000-3,000 mg/kg body weight/day (mkd). this design enables dose-response analysis and calculation of benchmark doses (bmdl). this reproduction study was preceded by a 28-day repeat dose subacute toxicity study, at 0-30-100-300 mkd. major effects in the reproduction stud ... | 2008 | 18255212 |
| radical prostatectomy in men aged >or=70 years: effect of age on upgrading, upstaging, and the accuracy of a preoperative nomogram. | to determine the effect of age on clinicopathological features, the accuracy of the preoperative nomogram, and survival after radical retropubic prostatectomy (rrp), as there are limited data on elderly men undergoing rrp. | 2008 | 18257855 |
| [a 20-year retrospective histoclinical analysis of parotid gland tumors in the ent department am in katowice]. | salivary gland neoplasms represent the diverse group of tumours encountered by the head and neck surgeons. | 2007 | 18260221 |
| [larynx and hypopharynx cancer morbidity in west pomerania region in the years 1990-2000]. | 1413 patient (1236 males and 177 females) with cancer of larynx or hypopharynx were treated in the ent department of the pomeranian medical academy in szczecin from 1990 to 2000. the patients were aged between 26 and 87 years. in the paper, based on the review of the cases history the following aspects have been determinated: incidence rate of the laryngeal cancer in the west pomerania region, cigarette smoking, size and primary site of the tumor, nodular metastases and clinical stage of the dis ... | 2007 | 18260227 |
| [malignant tumors of the nose and paranasal sinuses in the years 1971-2005 in the material of the otolaryngology clinic wim]. | the aim of this study was retrospective analysis of evidence concerning to malignant tumors of the nasal cavity and the paranasal sinuses diagnosed in otolaryngology clinic of the military institute of the health service 1971-2005. the methods of therapy were also discussed. | 2007 | 18260254 |
| chordoma of a thoracic vertebra. a case report. | a 51-year-old woman presented with aspecific clinical symptoms of the thoracic spine. radiological survey showed a tumour mass at t3-t4. initial microscopic evaluation was suggestive of renal cell carcinoma metastasis. lack of a primary tumour and revision of the specimens changed the diagnosis into chordoma. | 2007 | 18260501 |
| surveillance for hepatocellular carcinoma in patients with cirrhosis improves outcome. | liver transplantation has become an effective treatment for cirrhotic patients with early-stage hepatocellular carcinoma. we hypothesized that the quality of surveillance for hepatocellular carcinoma influences prognosis by affecting access to liver transplantation. | 2008 | 18261500 |
| two-generation reproductive toxicity study of the flame retardant hexabromocyclododecane in rats. | male and female rats were fed a diet containing flame retardant hexabromocyclododecane (hbcd) at 0, 150, 1500 or 15,000 ppm throughout the study beginning at the onset of a 10-week pre-mating period and continuing through the mating, gestation and lactation periods for two generations. the mean daily intakes of hbcd during the whole period of administration were 10.2, 101 and 1008 mg/kg bw in f0 males, 14.0, 141 and 1363 mg/kg bw in f0 females, 11.4, 115 and 1142 mg/kg bw in f1 males, and 14.3, ... | 2008 | 18262388 |
| effects of various antidepressants on serum thyroid hormone levels in patients with major depressive disorder. | a total of 62 patients with major depressive disorder were analyzed in the study. patients were evaluated for 11 weeks in an open label design to investigate the differential effects of reboxetine, sertraline and venlafaxine on thyroid hormones. serum thyrotrophin (tsh), thyroxine (t4) and free (f)t4 levels were measured before and after treatment. all groups showed significant improvement in ham-d scores. tsh level significantly reduced and t4 level significantly increased in the reboxetine gro ... | 2008 | 18262705 |
| phosphatases and kinases. | the reaction conditions and applications of two phosphatases and one kinase are described in this unit. bacterial alkaline phosphatase (bap) from e. coli and calf intestine phosphatase (cip) from veal are commonly used in nucleic acid research. both enzymes catalyze the hydrolysis of 5'-phosphate residues from dna, rna, and ribo- and deoxyribonucleoside triphosphates. the dephosphorylated products possess 5'-hydroxyl termini which can subsequently be radioactively labeled using [gamma-32p]atp an ... | 2001 | 18265219 |
| dna ligases. | dna ligases catalyze the formation of phosphodiester bonds between juxtaposed 5' phosphate and a 3'-hydroxyl terminus in duplex dna. this activity can repair single-stranded nicks in duplex dna and join duplex dna restriction fragments having either blunt ends or homologous cohesive ends. two ligases are used for nucleic acid research and their reaction conditions and applications are described in this unit: e. coli ligase and t4 ligase. these enzymes differ in two important properties. one is t ... | 2001 | 18265223 |
| rna ligases. | t4 rna ligase, the product of the phage gene 63, is purified from phage-infected cells. it catalyzes the atp-dependent covalent joining of single-stranded 5'-phosphoryl termini of dna or rna to single-stranded 3'-hydroxyl termini of dna or rna. this unit describes specific reaction conditions as well as applications such as radioactive labeling of 3' termini of rna, circularizing deoxy- and ribo-oligonucleotides, ligating oligomers for oligonucleotide synthesis, and stimulating the blunt-end lig ... | 2001 | 18265224 |
| dna-dependent dna polymerases. | this unit presents characteristics and reaction conditions of the dna-dependent dna polymerases, including e. coli dna polymerase i, klenow fragment of e. coli dna polymerase i, t4 dna polymerase, native and modified t7 dna polymerase, and taq dna polymerase. | 2001 | 18265231 |
| cloning of small rna molecules. | small rnas that are derived from dsrna precursors act as guide rnas during sequence-specific epigenetic regulation of eukaryotic gene expression. these small regulatory rnas are between 20 and 30 nucleotides in length, and fall into one or more of the following categories: small interfering rnas (sirnas), micrornas (mirnas), and heterochromatic sirnas (hsirnas). procedures to record the profile of small rnas expressed in cultured cells or tissues are described. the small rnas are directionally c ... | 2005 | 18265364 |
| thyroid dysfunction in patients with down syndrome. | the close relationship between thyroid disorder and down syndrome (ds) had been widely reported in the literature. the aim of this study was to assess the prevalence rate and the pattern of thyroid dysfunction in ds in taiwan. | 2007 | 18265539 |
| is sympathectomy at t4 level better than at t3 level for treating palmar hyperhidrosis? | we compared the results from a video-assisted thoracoscopic sympathectomy (vts) at the t4 denervation level with those from a vts at the t3 level for the treatment of palmar hyperhydrosis (ph). | 2008 | 18266585 |
| the impact of spontaneous tumour perforation on outcome following colon cancer surgery. | the impact of spontaneous tumour perforation on survival following surgery for colon cancer is unclear. this study compares survival outcomes for patients with perforated colonic cancer with stage-matched nonperforated cancer. | 2008 | 18266887 |
| effect of thyroid hormone concentration on the transcriptional response underlying induced metamorphosis in the mexican axolotl (ambystoma). | thyroid hormones (th) induce gene expression programs that orchestrate amphibian metamorphosis. in contrast to anurans, many salamanders do not undergo metamorphosis in nature. however, they can be induced to undergo metamorphosis via exposure to thyroxine (t4). we induced metamorphosis in juvenile mexican axolotls (ambystoma mexicanum) using 5 and 50 nm t4, collected epidermal tissue from the head at four time points (days 0, 2, 12, 28), and used microarray analysis to quantify mrna abundances. | 2008 | 18267027 |
| regionalisation of the muscle fascicle architecture in the equine longissimus dorsi muscle. | the longissimus dorsi is the largest muscle in the equine back and plays an important role in locomotor ability and performance in the horse. in vivo studies suggest that the mechanical function varies between different muscle segments, in part determined by anatomy. it is possible therefore that variations in function reflect variations in the anatomy of the longissimus dorsi along its length. | 2008 | 18267885 |
| the concerted action of lactoferrin and bacteriophages in the clearance of bacteria in sublethally infected mice. | both lactoferrin (lf) and bacteriophages are potent antibacterial agents. lf is contained in the secretory fluids of mammals and bacteriophages are specific bacterial viruses. | 2008 | 18268472 |
| ovarian hyperstimulation syndrome caused by an fsh-secreting pituitary adenoma. | a 40-year-old woman presented with galactorrhea and oligomenorrhea. she had a history of multiple ovarian cysts and pelvic pain. | 2008 | 18268519 |
| serum n-terminal-pro-b-type natriuretic peptide (nt-pro-bnp) levels in hyperthyroidism and hypothyroidism. | e natriuretic peptides represent a novel diagnostic tool in the assessment of heart failure. n-terminal-pro-b-type natriuretic peptide (nt-probnp), a member of the natriuretic peptid family, is produced and released from cardiac ventricles. changes in cardiac functions are observed in thyroid dysfunctions. the aim of this study was to assess the changes in serum nt-probnp levels and to evaluate impact of thyroid hormones on serum nt-probnp in patients with hyperthyroidism and hypothyroidism. ser ... | 2007 | 18271501 |
| rapid isolation and identification of bacteriophage t4-encoded modifications of escherichia coli rna polymerase: a generic method to study bacteriophage/host interactions. | bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial rna polymerase. using a rapid, specific, single-step affinity isolation procedure to purify escherichia coli rna polymerase from bacteriophage t4-infected cells, we have identified bacteriophage t4-dependent modifications of the host rna polymerase. we suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alteration ... | 2008 | 18271525 |
| atomic force microscopy reveals parallel mechanical unfolding pathways of t4 lysozyme: evidence for a kinetic partitioning mechanism. | kinetic partitioning is predicted to be a general mechanism for proteins to fold into their well defined native three-dimensional structure from unfolded states following multiple folding pathways. however, experimental evidence supporting this mechanism is still limited. by using single-molecule atomic force microscopy, here we report experimental evidence supporting the kinetic partitioning mechanism for mechanical unfolding of t4 lysozyme, a small protein composed of two subdomains. we observ ... | 2008 | 18272500 |
| endocrine factors and ovarian follicles are influenced by body condition and somatotropin in postpartum beef cows. | multiparous beef (1/4 to 3/8 bos indicus; n = 99) cows were managed to achieve low (bcs = 4.3 +/- 0.1; n = 50) or moderate (bcs = 6.1 +/- 0.1; n = 49) body condition (bc) to determine the influence of bovine (b) st on the number of follicles, diameter of largest follicle, and serum concentrations of igf-i, triiodothy-ronine (t3), thyroxine (t4), and prolactin. beginning 32 d postpartum, cows within each bc were assigned randomly to treatment with or without bst. non-bst-treated cows received no ... | 2008 | 18272855 |
| performance, behavior, and welfare of friesian heifers housed in pens with two, four, and eight individuals per concentrate feeding place. | the objective of the present study was to examine the effects of increasing the number of heifers per concentrate feeding place on performance, behavior, welfare indicators, and ruminal fermentation of feedlot heifers. seventy-two friesian heifers were used in a factorial arrangement, with 3 treatments and 3 blocks of similar bw. treatments consisted of 2 (t2), 4 (t4), or 8 (t8) heifers per each place in the concentrate feeder (8 heifers/pen). concentrate and straw were fed at 0830 h in individu ... | 2008 | 18272856 |
| café-au-lait macules and pediatric malignancy caused by biallelic mutations in the dna mismatch repair (mmr) gene pms2. | a 14-year-old male presented with a t4 sigmoid adenocarcinoma, <10 colonic adenomas and multiple café-au-lait macules. family history was not suggestive of a dominant hereditary form of colorectal cancer. evaluation of the tumor revealed abnormal immunohistochemical staining of the pms2 protein and high frequency microsatellite instability. germline analysis identified biallelic pms2 missense mutations. a new cancer syndrome caused by biallelic mutations in the mismatch repair genes, including p ... | 2008 | 18273873 |
| psychoneuroendocrine effects of combined thyroxine and triiodothyronine versus tyrosine during prolonged antarctic residence. | we previously reported that cognitive function improves with thyroxine and that there is a circannual pattern to mood and human tsh during antarctic residence. to extend these findings, we examined the effects of tyrosine and a combined levothyroxine/liothyronine supplement in euthyroid men and women who spent the austral summer (n = 43) and/or winter (n = 42) in antarctica. | 2007 | 18274206 |
| leiomyosarcoma of the anal canal: case report and review of the literature. | a rare case of a leiomyosarcoma of the anal canal is presented. a 68-year-old male presented with painful defecation and rectal bleeding. endorectal ultrasound revealed a mass invading both sphincters (t4 lesion), and extending 6 cm into the anal canal. colonoscopy revealed an ulcerated area in the anal canal, of which biopsies revealed high-grade leiomyosarcoma--only the eighteenth such case at the time of this submission. the details of the case and teaching images are presented in detail, as ... | 2008 | 18274436 |
| diagnosing unsuspected hypopituitarism in adults from suggestive thyroid function test results. | the typical pattern of thyroid function tests (tfts) associated with hypopituitarism consists of subnormal free t4 (ft4) or total t4 and normal or marginally elevated thyroid-stimulating hormone (tsh). a previous study calculated an incidence of hypopituitarism of 3.2 cases/100,000/year by following up abnormal tfts. the aim of this study was to verify the incidence of unsuspected hypopituitarism diagnosed by reflective testing on such samples in a scottish population. | 2008 | 18275677 |
| de novo high-resolution protein structure determination from sparse spin-labeling epr data. | as many key proteins evade crystallization and remain too large for nuclear magnetic resonance spectroscopy, electron paramagnetic resonance (epr) spectroscopy combined with site-directed spin labeling offers an alternative approach for obtaining structural information. such information must be translated into geometric restraints to be used in computer simulations. here, distances between spin labels are converted into distance ranges between beta carbons by using a "motion-on-a-cone" model, an ... | 2008 | 18275810 |
| the effect of alendronate on progression of spinal osteophytes and disc-space narrowing. | bisphosphonates may have chondroprotective effects that could be of relevance in osteoarthritis. using data from a large fracture prevention trial, we evaluated the effect of alendronate on the progression of radiographic spinal osteophytes (ost) and disc-space narrowing (dsn). | 2008 | 18276744 |
| [combined spinal-epidural anesthesia for cesarean section in a patient with dilated cardiomyopathy]. | we report successful anesthetic management of elective cesarean section in a 31-year-old patient with dilated cardiomyopathy (dcm) using combined spinal-epidural anesthesia (csea). after inserting an arterial catheter and central venous catheter, isobaric bupivacaine (0.5% ; 5 mg) with fentanyl 10 microg was injected intrathecally at the l4-5 interspace under administration of dopamine 3 microg kg(-1) min(-1). 10 min and later, a total of ropivacaine (0.5%; 70 mg) with fentanyl 50 microg was tit ... | 2008 | 18277568 |
| [thyroid hormone level change in patients undergoing oral and maxillofacial surgery]. | to study the changes of thyroid hormone level in patients undergoing oral and maxillofacial surgery before and after operation, in order to provide some evidences for postoperative treatment and recovery. | 2007 | 18278413 |
| [comparison of translucency between enamel, dentin and vita veneer porcelain]. | to compare the translucency between enamel, dentin and dental porcelain. | 2007 | 18278420 |
| metabolic effects of thyroid hormone derivatives. | the processes and pathways mediating the intermediary metabolism of carbohydrates, lipids, and proteins are all affected by thyroid hormones (ths) in almost all tissues. particular attention has been devoted by scientists to the effects of ths on lipid metabolism. among others, effects related to cholesterol, lipid handling, and cardiac performance have been the subject of study. many reports are present in the literature concerning the calorigenic effect of ths, with most of them aimed at ident ... | 2008 | 18279024 |
| [value of multislice spiral ct in preoperative staging of rectal carcinoma]. | preoperative staging is important for optimal therapy planning and prognosis prediction of rectal carcinoma. the role of conventional computed tomography (ct) in preoperative staging of rectal carcinoma is controversial. this study was to evaluate the value of multislice spiral computed tomography (msct) in preoperative staging of rectal carcinoma. | 2008 | 18279621 |
| thyroid gland development and defects. | during the functional ontogenesis of the thyroid gland an increasing number of transcription factors play fundamental roles in thyroid-cell differentiation, maintenance of the differentiated state, and thyroid-cell proliferation. the early growth and development of the fetal thyroid appears to be generally independent of thyroid-stimulating hormone (tsh). tsh and thyroxine (t4) levels increase from the 12th week of gestation until delivery, whereas triiodothyronine (t3) levels remain relatively ... | 2008 | 18279780 |
| postoperative stability after sagittal split ramus osteotomies for a mandibular setback with monocortical plate fixation or bicortical screw fixation. | this comparative study was performed to analyze mandibular stability after bilateral sagittal split ramus osteotomies for a mandibular setback with a monocortical plate fixation or bicortical screw fixation. | 2008 | 18280376 |
| oral squamous cell carcinoma margin discrepancy after resection and pathologic processing. | resecting oral squamous cell carcinoma (scc) with an appropriate margin of uninvolved tissue is critical in preventing local recurrence and making the decision regarding postoperative radiation therapy. this task can be difficult due to the discrepancy between margins measured intraoperatively and those measured microscopically by the pathologist after specimen processing. the goal of this study is to quantify and compare the amount of margin discrepancy observed based on tumor location and stag ... | 2008 | 18280387 |
| non-genomic actions of thyroid hormone in brain development. | thyroid hormone (th) is essential for neuronal migration and synaptogenesis in the developing brain. assembly of neuronal circuits depends on guidance cues provided by the extracellular matrix. these cues are interpreted by the migrating neuron and its growing neurites through transmembrane signaling proteins anchored in place by the actin cytoskeleton. one of the best examples of a non-genomic action of thyroid hormone is its dynamic regulation of the number and quantity of actin fibers in astr ... | 2008 | 18280526 |
| phage t4 segb protein is a homing endonuclease required for the preferred inheritance of t4 trna gene region occurring in co-infection with a related phage. | homing endonucleases initiate nonreciprocal transfer of dna segments containing their own genes and the flanking sequences by cleaving the recipient dna. bacteriophage t4 segb gene, which is located in a cluster of trna genes, encodes a protein of unknown function, homologous to homing endonucleases of the giy-yig family. we demonstrate that segb protein is a site-specific endonuclease, which produces mostly 3' 2-nt protruding ends at its dna cleavage site. analysis of segb cleavage sites sugges ... | 2008 | 18281701 |
| prostate cancer in renal transplant recipients. | we conducted a retrospective multi-centre study to determine the characteristics of prostate cancer in renal transplant recipients (rtr) and to analyse the relation with immunosuppressive maintenance therapies. | 2008 | 18283085 |
| intra-arterial chemoradiotherapy for locally advanced oral cavity cancer: analysis of therapeutic results in 134 cases. | the objective of this study was to investigate the therapeutic results of arterial injection therapy via the superficial temporal artery for 134 cases of stages iii and iv (m0) oral cavity cancer retrospectively, and to clarify the prognostic factors. we administered intra-arterial chemoradiotherapy by continuous infusion of carboplatin in 65 cases from january 1993 to july 2002. systemic chemotherapy was performed on 26 cases at the same time. we administered intra-arterial chemoradiotherapy by ... | 2008 | 18283309 |
| [procaryotic expression, purification and identification of recombinant human prostate-specific antigen]. | to produce recombinant human prostate-specific antigen (psa) by molecular cloning technology and to identify its activity. | 2007 | 18284054 |
| long-term outcome of 495 tnm stage i or ii patients with differentiated thyroid carcinoma followed up with neck ultrasonography and thyroglobulin measurements on t4 treatment. | recent guidelines for surveillance of patients with differentiated thyroid carcinoma emphasize tsh-stimulated serum thyroglobulin (tg) measurements and neck ultrasonographies (us) over tg measurements on t(4) and diagnostic whole body scans (dxwbs). we evaluated the safety and efficacy of a surveillance paradigm comprising yearly t(4)-suppressed tg measurements and neck us every second year, with an increase in tg to a detectable level being a prompt indication for dxwbs. | 2008 | 18284635 |
| triiodothyronine levels in athyreotic individuals during levothyroxine therapy. | thyroidal production of triiodothyronine (t3) is absent in athyreotic patients, leading to the suggestion that t3 deficiency may be unavoidable during levothyroxine (lt4) therapy. however, trials evaluating therapy with combined lt4 and t3 have failed to demonstrate any consistent advantage of combination therapy. | 2008 | 18285588 |
| thyrotoxic periodic paralysis. | a young man of 30 yrs got himself admitted in bangabandhu sheikh mujib medical university (bsmmu) hospital, dhaka, bangladesh with the complaints of suddenly developed weakness in his all four limbs. he had features of hyperthyroidism and he gave the history of similar attacks of weakness in his all four limbs in the previous months. his potassium was in the lower part of the normal range and his t4 and t3 were elevated but tsh was markedly low. he was diagnosed as a case of thyrotoxic periodic ... | 2008 | 18285742 |
| identification of acanthamoeba genotype t4 and paravahlkampfia sp. from two clinical samples. | in this study, two free-living amoebae strains, acanthamoeba genotype t4 and paravahlkampfia sp., which were isolated from keratitis cases are presented. while the acanthamoeba strain was isolated as a single agent, the paravahlkampfia strain was found together with herpes simplex virus. neither of the patients were contact lens wearers, but they did have a history of minor corneal trauma. amoebae were detected on non-nutrient agar covered with escherichia coli. based on pcr-amplified 18s rrna-g ... | 2008 | 18287307 |
| clinicopathological significance of cyclooxygenase-2 expression in hypopharyngeal squamous cell carcinoma. | the purpose of this study was to determine the expression of cyclooxygenase-2 (cox-2) in normal epithelium, dysplasia and squamous cell carcinoma of the hypopharynx and to investigate associations with clinicopathological factors and survival. seventy-five patients with hypopharyngeal squamous cell carcinomas (hpscc) who underwent surgical treatment at the department of otolaryngology, osaka medical center for cancer and cardiovascular diseases, were investigated. cox-2 expression was determined ... | 2008 | 18288396 |
| decreased expression of runx3 is correlated with tumor progression and poor prognosis in patients with esophageal squamous cell carcinoma. | runt-related transcription factor 3 (runx3) has been reported to be a candidate tumor suppressor gene in gastric cancer. however, in esophageal cancer, the role of runx3 has not been studied. the expression of runx3 mrna was quantified by real-time reverse transcription polymerase chain reaction using taq man pcr in 15 esophageal cancer cell lines (te1-15) and 70 esophageal squamous cell carcinoma (escc) specimens and their paired normal esophageal mucosa. the data were analyzed with reference t ... | 2008 | 18288406 |
| anatomy of thoracic splanchnic nerves for surgical resection. | thoracic splanchnic nerves conduct pain sensation from the abdominal organs around the celiac ganglion. splanchnicectomy is the procedure used mainly for the control of intractable visceral pain. forty-six human posterior thoracic walls were dissected. the formation pattern, course, and incidence of communication of the thoracic splanchnic nerves were investigated. the greater splanchnic nerves (gsns) were formed by nerve branches from the t4-t11 thoracic sympathetic ganglia and the most common ... | 2008 | 18288763 |
| rna base damage and repair. | elaborate repair pathways counteract the deleterious effects of dna damage by mechanisms that are understood in reasonable detail. in contrast, repair of damaged rna has not been widely explored. this may be because aberrant rnas are generally assumed to be degraded rather than repaired. the reason for this view is well founded, since conserved surveillance mechanisms that degrade abnormal rnas are thoroughly documented. numerous proteins and protein-rna complexes are involved in the metabolism ... | 2007 | 18289040 |
| epidural lidocaine-bicarbonate-adrenaline vs levobupivacaine for emergency caesarean section: a randomised controlled trial. | epidural mixtures containing lidocaine with or without additives are commonly used to convert epidural analgesia in labour to anaesthesia for emergency caesarean section, but direct comparisons with alternative, single agents in this situation are few. in a prospective double-blinded trial, we compared a freshly prepared lidocaine-bicarbonate-adrenaline mixture (final concentrations 1.8%, 0.76% and 1:200,000, respectively) with our standard agent, levobupivacaine 0.5%, for extending epidural blo ... | 2008 | 18289229 |
| orally delivered foot-and-mouth disease virus capsid protomer vaccine displayed on t4 bacteriophage surface: 100% protection from potency challenge in mice. | an orally delivered foot-and-mouth disease (fmd) vaccine has not previously been reported. by using a t4 bacteriophage nanoparticle surface gene-protein display system (t4-s-gpds), we created a foot-and-mouth disease virus (fmdv) entire capsid protein vaccine candidate. on the t4 phage surface soc site, a full length fmdv capsid precursor polyprotein (p1, 755 aa) and proteinase 3c (213 aa) derived from an infected pig of serotype o strain gd-10 (1999), were separately displayed on different t4 p ... | 2008 | 18289743 |
| cardiac output derived from arterial pressure waveform analysis in patients undergoing cardiac surgery: validity of a second generation device. | the performance of a recently introduced, arterial waveform-based device for measuring cardiac output (co) without the need of invasive calibration (flotrac/vigileo) has been controversial. we designed the present study to assess the validity of an improved version of this monitoring technique compared with intermittent thermodilution co measurement using a pulmonary artery catheter in patients undergoing cardiac surgery. | 2008 | 18292432 |
| influence of timed nutrient diet on depression and light sensitivity in seasonal affective disorder. | seasonal affective disorder (sad) patients crave and eat more carbohydrates (cho) in fall-winter when depressed, especially in the evenings, and feel energetic thereafter. evening cho-rich meals can phase delay circadian rhythms, and glucose increases retinal response to light. we studied timed cho- or protein-rich (prot) diet as a putative therapy for sad. unmedicated, dsm-iv-diagnosed depressed women with sad (n=22, 19-63 yrs) in the follicular phase of the menstrual cycle (present in 19) were ... | 2008 | 18293149 |
| direct nmr detection of alkali metal ions bound to g-quadruplex dna. | we describe a general multinuclear (1h, 23na, 87rb) nmr approach for direct detection of alkali metal ions bound to g-quadruplex dna. this study is motivated by our recent discovery that alkali metal ions (na+, k+, rb+) tightly bound to g-quadruplex dna are actually "nmr visible" in solution (wong, a.; ida, r.; wu, g. biochem. biophys. res. commun. 2005, 337, 363). here solution and solid-state nmr methods are developed for studying ion binding to the classic g-quadruplex structures formed by th ... | 2008 | 18293981 |
| the effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. | investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (cssv) disease free clonal propagules from infected trees. polymerase chain reaction (pcr) capillary electrophoresis revealed the presence of cssv in all the callus tissues induced from the cssv-infected amelonado cocoa trees (t1, t2 and t4). the virus was transmitted to primary somatic embryos induced from the infected callus tissues at the rate of 10 (19%), 18 (14%) and 16 (15%) for t1, t2 ... | 2008 | 18294704 |
| spinal cord compression caused by vertebral hemangioma being symptomatic during pregnancy. | hemangioma is one of the most common benign tumors of the spine, and it remains silent in the vast majority of subjects afflicted. pregnancy is a known risk factor for symptomatic conversion of the previously silent vertebral hemangiomas. however, the occurrence is rare with only 26 cases reported in the english medical literature. | 2009 | 18295858 |
| transient congenital hypothyroidism in an iodine-replete area is not related to parental consanguinity, mode of delivery, goitrogens, iodine exposure, or thyrotropin receptor autoantibodies. | to assess transient congenital hypothyroidism (tch) etiologies in two iranian cities. | 2008 | 18296902 |
| subclinical hypothyroidism: the state of the art. | subclinical hypothyroidism (sh) is a common clinical problem, particularly in adulthood and the elderly. its prevalence is conditioned by several etiological and risk factors. the highest age- and sex-specific rates are in women over 60. sh may be associated with manifestations of mild thyroid failure, which may reverse under levothyroxine (l-t4) therapy. the risk of progression to overt hypothyroidism is distinctly higher in cases with underlying thyroid disease. a population routine screening ... | 2008 | 18296910 |
| the role of iron deficiency in persistent goiter. | iodine deficiency has been identified as a significant public health problem in iran. the main strategy for controlling iodine deficiency was nationwide salt iodination. over 10 years after starting this program, goiter is still endemic in school children. iron deficiency may have interfered with the iodine intervention program. the objective of the present study was to evaluate the relationships between iron status, thyroid hormone profile, and the prevalence of goiter 11 years after implementa ... | 2008 | 18298292 |
| possible effects of environmental nitrates and toxic organochlorines on human thyroid in highly polluted areas in slovakia. | heavy environmental pollution resulting from uncontrolled industrial and agricultural activities has occurred in several areas of slovakia. so far, field surveys focused mainly on the thyroid have been conducted in one area polluted by nitrates and in a large area polluted mainly by organochlorinated toxicants. in children from the high nitrate area (hna, n = 324) significantly higher thyroid volume (thv) by ultrasound was found compared with age-matched children from surrounding areas with low ... | 2008 | 18298316 |
| rapid quantification of total viral dna in the supernatants of activated sludge samples with the fluorescent dye picogreen. | to develop a rapid and simple method for quantifying viral dna concentrations and determining viral quantities in activated sludge. | 2008 | 18298452 |
| placement of pedicle screws in thoracic idiopathic scoliosis: a magnetic resonance imaging analysis of screw placement relative to structures at risk. | in posterior pedicle screw instrumentation of thoracic idiopathic scoliosis, screw malposition might cause significant morbidity in terms of possible pleural, spinal cord, and aorta injury. preoperative axial magnetic resonance images (mri) in 12 consecutive patients with right thoracic adolescent scoliosis, all with king type 3 curves, were analyzed in order to evaluate the relationship between the inserted pedicle screw position to pleura, spinal cord, aorta. axial vertebral images for each th ... | 2008 | 18301931 |