| patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. | infection of domestic swine with highly virulent, classical swine fever virus (csfv) strain brescia, causes lethal disease in all infected animals. however, the molecular mechanisms involved in modulating the host cellular processes and evasion of the immune response have not been clearly established. to gain insight into, the early host response to csfv, we analyzed the pattern of gene expression in infected swine macrophages, using custom designed swine microarrays. macrophages, the target cel ... | 2010 | 20302897 |
| treatment with bioartificial liver improves lung injury in a swine model of partial hepatectomy and ischemia/reperfusion. | hepatic ischemia/reperfusion injury can lead to remote lung injury by inducing oxidative stress and inflammation. this study aims to investigate whether support of liver function with a bioartificial liver can attenuate remote lung injury after extended hepatectomy. | 2010 | 20306437 |
| [haemorrhagic septicaemia in a pig caused by extraintestinal pathogenic escherichia coli (expec) as a differential diagnosis in classical swine fever--case report and review of the literature]. | domestic pig herds in some regions of germany are permanently threatened by classical swine fever. in the case of suspicion, a series of infectious and non infectious causes has to be excluded. the present paper describes a case of escherichia coli septicaemia, with clinical and pathological symptoms that could not be differentiated from european or african swine fever. the e. coli strain could not be classified by standard serotyping. virulence factors common for etec (enterotoxic e. coli) or e ... | 2010 | 20329644 |
| a gel-free approach in vascular smooth muscle cell proteome: perspectives for a better insight into activation. | abstract: | 2010 | 20334645 |
| [radiofrequency ablation as a palliative therapy option in ent tumors: in vivo and in vitro testing]. | high frequency thermotherapy (hftt) is an established palliative therapy for hepatic malignancies. an in vivo and in vitro trial examined the preconditions for the application of hftt with liquid-cooled wet electrodes for minimally invasive palliation of head and neck tumors. | 2010 | 20336271 |
| the optimal phasic relationship between synchronized shock and mechanical chest compressions. | pauses for shock delivery in chest compressions are detrimental to the success of resuscitation and may be eliminated with the use of mechanical chest compressors. however, the optimal phasic relationship between mechanical chest compression and defibrillation is still unknown. we therefore undertook a study to assess the effects of timing of defibrillation in the mechanical chest compression cycle on the defibrillation threshold (dft) using a porcine model of cardiac arrest. | 2010 | 20346567 |
| qualitative and quantitative distribution of pcv2 in wild boars and domestic pigs in germany. | porcine circovirus 2 (pcv2), the causative agent of postweaning multisystemic wasting syndrome (pmws), has been detected in north american and european wild boars at prevalences arguing for high circulation rates among populations. systematic data on the qualitative distribution of pcv2 infections and on pcvd (pcv2 diseases) in wild boars are rare, however, and quantitative data about viral loads are missing. to be able to judge the pcv2/pcvd situation in wild boars, evaluation of the nationwide ... | 2010 | 20346600 |
| glucagon-like peptide-1 (glp-1) attenuates post-resuscitation myocardial microcirculatory dysfunction. | post-resuscitation syndrome leads to death in approximately 2 out of every 3 successfully resuscitated victims, and myocardial microcirculatory dysfunction is a major component of this syndrome. the aim of this study was to determine if glucagon-like peptide-1 (glp-1) improves post-resuscitation myocardial microcirculatory function. | 2010 | 20347207 |
| calcium-dependent signalling is essential during collateral growth in the pig hind limb-ischemia model. | we investigated the effect of pharmacological activation of the ca(2+)-channel transient receptor potential cation channel, subfamily v, member 4 (trpv4) on collateral growth in a pig hind limb-ischemia model thereby identifying subcellular mechanisms. domestic pigs received femoral artery ligature and were randomly assigned to one of the following groups (each n=6): (1) 4alpha-phorbol 12,13-didecanoate (4alphapdd) treatment; (2) treatment with an arterio-venous shunt (av-shunt) distal to the oc ... | 2010 | 20363225 |
| studying phenotypic evolution in domestic animals: a walk in the footsteps of charles darwin. | charles darwin used domesticated plants and animals as proof of principle for his theory on phenotypic evolution by means of natural selection. inspired by darwin's work, we developed an intercross between the wild boar and domestic pigs to study the genetic basis for phenotypic changes during domestication. the difference in coat color is controlled by two major loci. dominant white color is due to two consecutive mutations in the kit gene: a 450-kb duplication and a splice mutation. black spot ... | 2010 | 20375320 |
| heterogeneous memory in restitution of action potential duration in pig ventricles. | restitution of action potential duration and memory importantly affect electrical stability in ventricles. studies have reported heterogeneous restitution among different regions of the ventricles. however, existence of heterogeneity in memory is not as well investigated. | 2010 | 20378123 |
| a novel approach for reinforcing the pancreatic remnant in laparoscopic distal pancreatectomy: an experimental study on a porcine model. | the major postoperative complication after open and laparoscopic distal pancreatectomy is pancreatic fistula. different operative techniques have been tested to minimize this unpleasant complication. we evaluated a new technique for pancreatic stump reinforcement with synthetic glue after laparoscopic distal pancreatectomy. ten female domestic pigs were divided into 2 groups; in group a (n=5), the pancreas was transected using an endogia stapler, whereas in group b (n=5), the pancreas was transe ... | 2010 | 20393319 |
| development and validation of a sensitive hplc method for the quantification of hi-6 in guinea pig plasma and evaluated in domestic swine. | a rapid and small volume assay to quantify hi-6 in plasma was developed to further the development and licensing of an intravenous formulation of hi-6. the objective of this method was to develop a sensitive and rapid assay that clearly resolved hi-6 and an internal standard in saline and plasma matrices. a fully validated method using ion-pair hplc and 2-pam as the internal standard fulfilled these requirements. small plasma samples of 35 microl were extracted using acidification, filtration an ... | 2010 | 20395179 |
| comparative animal study on hard tissue integration and bone formation of different nobel biocare implants. | dental implantation aims at optimal and long-term hard tissue integration. beside primary stability, loading time and other factors, e.g. the surface of the endosteal part of the implant, is a matter of special importance. in this animal trial, hard tissue integration of two different implant types was studied using radiological, histological and histomorphometric analysis. two different implants with an oxidized surface (tiunite; nobel biocare ab, goteborg, sweden, nobelreplace tapered groovy 4 ... | 2009 | 20400804 |
| pathomorphological changes after radiofrequency ablation in the liver. | radiofrequency ablation (rfa) has become a widespread treatment option for liver carcinoma. there is limited knowledge regarding the macroscopic and histomorphological changes of induced lesions. twelve domestic pigs underwent rfa using a starburst xl device with ablation diameter of 3 cm. one animal died within 24 h, two animals were killed after 2 weeks, and nine after 4 weeks. their livers were used for macroscopic and histological investigation. six human liver resection specimens after prev ... | 2010 | 20403040 |
| patterns of east asian pig domestication, migration, and turnover revealed by modern and ancient dna. | the establishment of agricultural economies based upon domestic animals began independently in many parts of the world and led to both increases in human population size and the migration of people carrying domestic plants and animals. the precise circumstances of the earliest phases of these events remain mysterious given their antiquity and the fact that subsequent waves of migrants have often replaced the first. through the use of more than 1,500 modern (including 151 previously uncharacteriz ... | 2010 | 20404179 |
| light microscopic, electron microscopic, and immunohistochemical comparison of bama minipig (sus scrofa domestica) and human skin. | here we sought to evaluate the possibility of using chinese bama miniature pig skin as a suitable animal model for human skin. morphologic features of the skin of bama miniature pigs resemble those of human skin, including skin layer thickness, development of a superficial vascular system, structure of the dermal-epidermal interface, and extracellular matrix. the characteristics and densities of langerhans cells, fibroblasts, vascular endothelial cells, and mast cells were similar between bama p ... | 2010 | 20412690 |
| influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in sus scrofa. | the aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. the goal of in vitro part of work was to observe influence of 7-ketocholesterol on inos and mip1 beta production in bronchoalveolar lavage fluid (balf) cells. we used 17 healthy domestic pigs randomly assigned into two treatment groups: group i with mechanical ventilation with physiological parameters; group || underwent injuri ... | 2010 | 20423610 |
| temporal distribution of ten calyptrate dipteran species of medicolegal importance in rio de janeiro, brazil. | brazilian studies involving entomological succession patterns in carcasses have been used to describe the necrophagous entomofauna of a determined municipality or region with forensic objectives. following the same objectives, an ecological study with 10 calyptrate dipterans was carried out during the winter of 2007 and the summer of 2008 in the metropolitan region of the municipality of rio de janeiro. the aim of this study was to describe several aspects of the phenology of these species in th ... | 2010 | 20428680 |
| correlation of right ventricular dysfunction parameters and pulmonary vascular obstruction score in acute pulmonary embolism in a porcine model. | this paper aims to non-invasively determine right ventricular dysfunction parameters (rvdp) in acute pulmonary embolism (ape) and to correlate these parameters with a computed tomography (ct)-based pulmonary artery obstruction score (os). in seven domestic pigs, magnetic resonance imaging (mri) was performed before (n = 7) and after (n = 5) ape was induced. analysis of relative changes in right ventricular stroke volume (rvsv%), output (rvo%), end-systolic (rvesv%), end-diastolic volume (rvedv%) ... | 2010 | 20428913 |
| endoscopic mucosal resection using a grasp-and-snare technique. | endoscopic mucosal resection (emr) is a minimally invasive method for en bloc removal of superficial gastrointestinal lesions. the aim of this study was to evaluate the feasibility of a novel grasp-and-snare emr technique. | 2010 | 20432205 |
| diffuse reflectance spectroscopy for optical soft tissue differentiation as remote feedback control for tissue-specific laser surgery. | laser surgery does not provide haptic feedback for operating layer-by-layer and thereby preserving vulnerable anatomical structures like nerve tissue or blood vessels. diffuse reflectance spectra can facilitate remote optical tissue differentiation. it is the aim of the study to use this technique on soft tissue samples, to set a technological basis for a remote optical feedback system for tissue-specific laser surgery. | 2010 | 20432281 |
| a study of alternative splicing in the pig. | abstract: | 2010 | 20444244 |
| variation of aujeszky's disease viruses in wild swine in usa. | in the united states of america, aujeszky's disease (pseudorabies) has been eradicated from all domestic swine. some re-emergence of infection occurred as vaccine use diminished. sporadic outbreaks have also occurred because of the reservoir of infection in feral swine that have spread across the southern two-thirds of the country and hawaii. in order to be able to understand the origins of re-emerging virus, sequence analysis of variable genes in pseudorabies virus (prv) has been used to differ ... | 2010 | 20444559 |
| pure "cable-tie partial nephrectomy": a porcine model. | the standard surgical procedure for polar renal tumors is nephron-sparing surgery, currently performed laparoscopically in most cases. this technically challenging surgery involves clamping of the hilar vessels and warm kidney ischemia. this study tested the feasibility of performing polar nephrectomy using a cable tie without hilar clamping or any other hemostatic procedure. | 2010 | 20445994 |
| the amplitude spectrum area correctly predicts improved resuscitation and facilitated defibrillation with head cooling. | when systemic hypothermia was maintained before inducing cardiac arrest, the likelihood of successful defibrillation and meaningful survival was increased. when hypothermia is induced during cardiopulmonary resuscitation, mortality is also improved. with the introduction of the amplitude spectrum area as a predictor of the success of electrical defibrillation, we investigated the effect of preferential head cooling initiated coincident with cardiopulmonary resuscitation on amplitude spectrum are ... | 2008 | 20449903 |
| a comparison between head cooling begun during cardiopulmonary resuscitation and surface cooling after resuscitation in a pig model of cardiac arrest. | employing transnasal head-cooling in a pig model of prolonged ventricular fibrillation, we compared the effects of 4 hrs of head-cooling started during cardiopulmonary resuscitation with those of 8 hrs of surface-cooling started at 2 hrs after resuscitation on 96-hr survival and neurologic outcomes. | 2008 | 20449906 |
| intra-arrest rapid head cooling improves postresuscitation myocardial function in comparison with delayed postresuscitation surface cooling. | to compare resuscitation outcomes and myocardial function among intra-arrest head cooling, delayed surface cooling, and uncooled controls. | 2008 | 20449907 |
| neuroprotective effects of the inhalational anesthetics isoflurane and xenon after cardiac arrest in pigs. | neurologic outcome after cardiopulmonary resuscitation from cardiac arrest carries a poor prognosis and treatment options to ameliorate brain damage are limited. | 2008 | 20449916 |
| experimental infection of pigs with border disease virus isolated from pyrenean chamois (rupicapra pyrenaica). | between 2001 and 2007, several outbreaks of disease associated with border disease virus (bdv) infection were reported in the central pyrenees (northeast spain) and were associated with a major reduction in chamois (rupicapra pyrenaica) populations. at the same time, wild boars (sus scrofa) from the same area were found to be seropositive to this pestivirus, without showing clinical signs. the present study examines the susceptibility of domestic swine and the course of the infection with a bdv ... | 2010 | 20453208 |
| detection of porcine circovirus in rodents - short communication. | porcine circoviruses (pcv) are present worldwide, infecting domestic pigs and wild boars alike. studies under laboratory conditions indicated that pcv can be taken up by mice and the virus can replicate in these animals. the possible role of rodents in maintaining and transmitting pcv2 infection in the field has not been investigated yet. the present study reports the detection of pcv2, the pathogenic form of the virus, in mice and rats. a number of rodents, such as mice, rats and voles, were co ... | 2010 | 20460225 |
| brucella suis infection in domestic pigs and wild boar in croatia. | between the years 2000 and 2004, 93,107 sera from 1,997 pig herds in 11 regions of croatia were tested for the presence of antibodies against brucellosis. positive results were observed in 67 herds from seven regions (mean individual prevalence: approximately 1%; herd prevalence: 3.4%). the herds from all but two of the infected farms were reared outdoors and thus almost certainly came into contact with wildlife. from 2003 to 2004, 424 sera, which were randomly collected from hunted wild boar (s ... | 2009 | 20462164 |
| early atherosclerosis aggravates the effect of renal artery stenosis on the swine kidney. | atherosclerotic renal artery stenosis (aras) is increasingly identified in patients with end-stage renal disease. renal function in aras patients deteriorates more frequently than in nonatherosclerotic renal artery stenosis (ras). this study was designed to test the hypothesis that atherosclerosis modifies the relationship between single-kidney hemodynamics and function and the severity of stenosis. the degree of unilateral ras in domestic pigs (4 normal, 26 ras, and 22 aras) was correlated with ... | 2010 | 20462971 |
| familiarity breeds contempt: kangaroos persistently avoid areas with experimentally deployed dingo scents. | whether or not animals habituate to repeated exposure to predator scents may depend upon whether there are predators associated with the cues. understanding the contexts of habituation is theoretically important and has profound implication for the application of predator-based herbivore deterrents. we repeatedly exposed a mixed mob of macropod marsupials to olfactory scents (urine, feces) from a sympatric predator (canis lupus dingo), along with a control (water). if these predator cues were al ... | 2010 | 20463952 |
| epidemiological survey of trichinellosis in wild boar (sus scrofa) and fox (vulpes vulpes) in a french insular region, corsica. | the mediterranean island of corsica was considered trichinella-free until 2004, when t. britovi larvae were discovered in domestic pigs at meat inspection. one red fox was also found infected the same year and in the same area than the infected pigs. this last finding highlighted the presence of trichinellosis in corsican wildlife. a trichinella survey was thus performed in wild boar (sus scrofa) and fox (vulpes vulpes), the two large wild species present on the island, to determine prevalence o ... | 2010 | 20471753 |
| divergence time estimates of east asian and european pigs based on multiple near complete mitochondrial dna sequences. | the time since the divergence of european and east asian domestic pigs and wild boars has been estimated in several phylogenetic analyses, generally based on partial mitochondrial sequences or on a small number of complete mtdna sequences. in the present study, we obtained a refined estimate of this divergence time based on a set of 32 near-complete mtdna sequences from wild and domestic pigs of european and asian types, including 14 new and 18 previously published sequences. a weighted average ... | 2011 | 20477794 |
| analysis of the non-recombining y chromosome defines polymorphisms in domestic pig breeds: ancestral bases identified by comparative sequencing. | sequences from 20 amplicons representing nine different loci and 11369bp from the short arm of the pig y chromosome were compared using pools of dna from different european and chinese breeds. a total of 33 polymorphic sites were identified, including five indels and 28 single nucleotide polymorphisms (snps). three high frequency snps within the coding regions of sry were further analysed across 889 males representing 25 european and 25 asian breeds or lines, plus a european line of meishan. two ... | 2010 | 20477804 |
| mitochondrial dna diversity in wild boar from the primorsky krai region (east russia). | we have studied the cytochrome b gene and control region dna variability in 14 wild boars from the primorsky region, in the far east corner of russia. variability was low (π = 0.00248 overall) compared with the usual estimates in these loci, indicating that this is a rather closed population. seven haplotypes were found, and one was identical to a chinese wild boar. phylogeographically, the sequences clustered among several asian clades, primarily chinese domestic pigs and japanese and chinese w ... | 2011 | 20477807 |
| dietary consistency and the midline sutures in growing pigs. | the purpose of this study was to investigate the effects of reduced masticatory function on midline suture growth and morphology in growing pigs. | 2010 | 20477970 |
| [hepatitis e--a new zoonotic disease in germany?]. | hepatitis e virus (hev) is the most frequent pathogen of a fecal-oral transmitted hepatitis in humans in many developing countries. it is endemic in the middle east, in india, in southeast asia, central asia as well as in central and south america. it can be predominantly found in young adults. the mortality rate comes up to 2% whereas in pregnant women death rate can reach about 25%. until a few years ago, acute hepatitis e was thought to be rare in industrialized countries: most cases were fou ... | 2010 | 20496825 |
| an animal model for sinus floor elevation with great elevation heights. macroscopic, microscopic, radiological and micro-ct analysis: ex vivo. | different animal models are used for research and training concerning sinus floor elevation. however, there is little information regarding an animal model for elevation heights of approximately 10 mm. the aim of this study was to explore the anatomical aspects of the maxillary sinus of adult pigs and to investigate the suitability of this animal as a model for sinus floor elevation training and research with great elevation heights. | 2010 | 20497438 |
| cell distribution after intracoronary bone marrow stem cell delivery in damaged and undamaged myocardium: implications for clinical trials. | abstract : introduction : early randomized clinical trials of autologous bone marrow cardiac stem cell therapy have reported contradictory results highlighting the need for a better evaluation of protocol designs. this study was designed to quantify and compare whole body and heart cell distribution after intracoronary or peripheral intravenous injection of autologous bone marrow mononuclear cells in a porcine acute myocardial infarction model with late reperfusion. methods : myocardial infarcti ... | 2010 | 20504285 |
| molecular and serological survey of hepatitis e virus infection among domestic pigs in inner mongolia, china. | to evaluate the prevalence and characteristics of swine hepatitis e virus (hev) infection in inner mongolia, china, serum samples obtained from 356 2- to 4-month-old pigs on 14 farms in inner mongolia were tested for the presence of anti-hev antibodies and hev rna. overall, 186 pigs (52%) tested positive for anti-hev antibodies, while 30 pigs (8%) had detectable hev rna levels. the 30 hev isolates recovered from the viremic pigs were phylogenetically classified into genotype 4 and differed from ... | 2010 | 20512514 |
| rethinking cull boar transport. | | 2010 | 20514259 |
| avian influenza vaccination in chickens and pigs with replication-competent adenovirus-free human recombinant adenovirus 5. | protective immunity to avian influenza (ai) virus can be elicited in chickens by in ovo or intramuscular vaccination with replication-competent adenovirus (rca)-free human recombinant adenovirus serotype 5 (ad5) encoding ai virus h5 (adtw68.h5) or h7 (adcn94.h7) hemagglutinins. we evaluated bivalent in ovo vaccination with adtw68.h5 and adcn94.h7 and determined that vaccinated chickens developed robust hemagglutination inhibition (hi) antibody levels to both h5 and h7 ai strains. additionally, w ... | 2010 | 20521636 |
| chitosan arrests bleeding in major hepatic injuries with clotting dysfunction: an in vivo experimental study in a model of hepatic injury in the presence of moderate systemic heparinisation. | the purpose of this study was to explore the effectiveness of two chitosan formulations, omni-stat® granules and celox gauze®, in a model of major hepatic injury in the presence of clotting dysfunction. | 2010 | 20522310 |
| iron chelation for amelioration of liver ischemia-reperfusion injury. | liver resections are frequently associated with significant ischemia-reperfusion (i-r) injury of the liver remnant. the aim of this study was to investigate whether deferoxamine (dfo) can ameliorate i-r injury during major hepatectomies performed under vascular exclusion of the liver in a porcine model. twelve female domestic pigs were divided into control (n = 6) and dfo treatment (n = 6) groups and subjected to 150 min. liver ischemia followed by 70% hepatectomy and 24 hours reperfusion. pigs ... | 2010 | 20524816 |
| laser-induced thermotherapy (litt)--evaluation of a miniaturised applicator and implementation in a 1.0-t high-field open mri applying a porcine liver model. | to evaluate the feasibility and safety of a novel litt applicator for thermal ablation of liver malignancies in 1.0-t high-field open mri. | 2010 | 20526885 |
| a long arm for ultrasound: a combined robotic focused ultrasound setup for magnetic resonance-guided focused ultrasound surgery. | focused ultrasound surgery (fus) is a highly precise noninvasive procedure to ablate pathogenic tissue. fus therapy is often combined with magnetic resonance (mr) imaging as mr imaging offers excellent target identification and allows for continuous monitoring of fus induced temperature changes. as the dimensions of the ultrasound (us) focus are typically much smaller than the targeted volume, multiple sonications and focus repositioning are interleaved to scan the focus over the target volume. ... | 2010 | 20527572 |
| gastric lymphatic basin dissection for sentinel node biopsy using hybrid natural orifice transluminal endoscopic surgery (notes). | the aim of the present study was to describe a method of gastric lymphatic basin dissection for sentinel node biopsy using natural orifice transluminal endoscopic surgery with laparoscopic assistance (hybrid notes) in a porcine model. lymph node dissection was performed in three healthy female domestic farm pigs (each around 40 kg) between october, 2007, and december, 2007. the pigs were administered a general anesthetic and laparoscopy-guided transvaginal colpotomy was performed. a two-channel ... | 2010 | 20528682 |
| a new surgical trainer (bopt) improves skill transfer for anastomotic techniques in gastrointestinal surgery into the operating room: a prospective randomized trial. | a multifunctional skill trainer (berlin operation trainer, bopt) allows realistic training of conventional gastrointestinal (gi) surgical techniques. the aim of this prospective randomized study was to evaluate the training success and the potential for transfer of anastomotic techniques in gi surgery into the operating room using the bopt. | 2010 | 20533037 |
| cutaneous challenge with chemical warfare agents in the skh-1 hairless mouse (ii): effects of some currently used skin decontaminants (rsdl and fuller's earth) against liquid sulphur mustard and vx exposure. | using the hairless mouse screening model presented in the companion paper(1) the aim of this study was to assess two skin decontaminating systems: fuller's earth (fe) and reactive skin decontamination lotion (rsdl) against two extremely toxic chemical warfare agents that represent a special percutaneous hazard, sulphur mustard (sm) and o-ethyl-s-(2[di-isopropylamino]ethyl)methyl-phosphonothioate (vx). five minutes after being exposed on the back to either 2 µl of neat sulphur mustard or 50 µg.kg ... | 2011 | 20534641 |
| classical swine fever virus in south-eastern europe--retrospective analysis of the disease situation and molecular epidemiology. | classical swine fever (csf) is among the most important diseases of domestic pigs and causes great socio-economic losses. therefore, control of csf is given high priority within the european union, including financial support of concerted control actions in candidate and in potential candidate countries. unfortunately, from some of these countries information on the csf situation and related data is very limited. this study was undertaken to gather all available information on the domestic pig p ... | 2010 | 20541876 |
| 1.5:1 meshed alloderm bolsters for stapled rectal anastomoses does not provide any advantage in anastomotic strength in a porcine model. | the most feared complication of colorectal anastomoses is leaks resulting in severe morbidity. the concept of staple-line reinforcement is a growing area of interest. in this study, the authors evaluated the feasibility and effect of using 1.5:1 meshed alloderm to bolster end-to-end stapled rectal anastomoses in a porcine model. | 2011 | 20542954 |
| phylogenetic analysis of classical swine fever virus isolates from peru. | classical swine fever (csf) is considered to be endemic in peru with outbreaks reported to the world organization for animal health as recently as 2008 and 2009. nevertheless, little is known regarding the genetic subgroup(s) of csf virus that are circulating in peru or their relationship to recent csf viruses that have been isolated from neighbouring south american countries or other parts of the world. in this study, we molecularly characterize csf viruses that were isolated from domestic pigs ... | 2010 | 20545910 |
| carbon dioxide-enhanced ct-guided placement of aortic stent-grafts: feasibility in an animal model. | to test the feasibility of carbon dioxide (co(2))-enhanced computed tomography (ct)-guided placement of infrarenal abdominal aortic stent-grafts in an animal model. | 2010 | 20557172 |
| hypoxia-inducible factor 1-alpha release after intracoronary versus intramyocardial stem cell therapy in myocardial infarction. | we have investigated the effect of stem cell delivery on the release of hypoxia-inducible factor 1 alpha (hif-1alpha) in peripheral circulation and myocardium in experimental myocardial ischemia. closed-chest, reperfused myocardial infarction (mi) was created in domestic pigs. porcine mesenchymal stem cells (mscs) were cultured and delivered (9.8 +/- 1.2 x 10(6)) either percutaneously noga-guided transendocardially (group im) or intracoronary (group ic) 22 +/- 4 days post-mi. pigs without msc de ... | 2010 | 20560024 |
| familial hypercholesterolaemic downsized pig with human-like coronary atherosclerosis: a model for preclinical studies. | a manageable and reproducible large animal model of human-like coronary atherosclerosis is lacking but highly needed for translational research in percutaneous coronary interventions and imaging. farm pigs with familial hypercholesterolaemia develop advanced atherosclerosis in two to three years but then weigh >200 kg making them impractical and costly. we aimed at down-sizing this pig and accelerating coronary plaque development to make the model more useful and affordable. | 2010 | 20562079 |
| rapid intradermal delivery of liquid formulations using a hollow microstructured array. | the purpose of this work is to demonstrate rapid intradermal delivery of up to 1.5 ml of formulation using a hollow microneedle delivery device designed for self-application. | 2011 | 20582455 |
| anterior shear strength of the porcine lumbar spine after laminectomy and partial facetectomy. | degenerative lumbar spinal stenosis is the most common reason for lumbar surgery in patients in the age of 65 years and older. the standard surgical management is decompression of the spinal canal by laminectomy and partial facetectomy. the effect of this procedure on the shear strength of the spine has not yet been investigated in vitro. in the present study we determined the ultimate shear force to failure, the displacement and the shear stiffness after performing a laminectomy and a partial f ... | 2010 | 20582709 |
| depth of tissue ablation and residual thermal damage caused by a pixilated 2,940 nm laser in a swine skin model. | the purpose of this study was to assess the effects of fluence, pulse stacking, and multiple passes on the depth of injury caused by a fractionated er:yag laser in an in vivo farm pig model. design/material/methods: a fractionated 2,940 nm er:yag laser (pixel, alma lasers, caesarea, israel) was applied to the flank skin of a yorkshire cross pig. the 11 mmx11 mm handpiece was comprised of either 49 or 81 microbeams (200 microm diameter), depending on the tip configuration. there were six differen ... | 2010 | 20583246 |
| negative intratracheal pressure produced by esophageal detector devices causes tracheal wall collapse in a porcine cardiac arrest model. | esophageal detector devices (edds) impose negative pressure on the trachea or esophagus to verify endotracheal tube (ett) position. in cardiac arrest, the smooth muscle of the lower esophageal sphincter relaxes in a time-dependent and irreversible manner. if relaxation also occurs in the muscular posterior tracheal wall, it could predispose tracheal invagination or collapse with negative pressure, potentially yielding false-negative (tracheal ett, edd indicates esophagus) results. we compared 3 ... | 2010 | 20584869 |
| how pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane. | mammalian sperms are activated in the oviduct. this process, which involves extensive sperm surface remodelling, is required for fertilization and can be mimicked under in vitro fertilization conditions (ivf). | 2010 | 20585455 |
| fourier domain pump-probe optical coherence tomography imaging of melanin. | we report the development of a two-color fourier domain pump-probe optical coherence tomography (ppoct) system. tissue phantom experiments to characterize the system performance demonstrated imaging depths in excess of 725 microm, nearly comparable to the base optical coherence tomography system. ppoct a-line rates were also demonstrated in excess of 1 khz. the physical origin of the ppoct signal was investigated with a series of experiments which revealed that the signal is a mixture of short a ... | 2010 | 20588366 |
| developmental stage-specific imprinting of ipl in domestic pigs (sus scrofa). | imprinted in placenta and liver (ipl) gene has been identified as an imprinted gene in the mouse and human. its sequence and imprinting status, however, have not been determined in the domestic pigs. in the present study, a 259 base pair-specific sequence for ipl gene of the domestic pig was obtained and a novel snp, a t/c transition, was identified in ipl exon 1. the c allele of this polymorphism was found to be the predominant allele in landrace,yorkshire, and duroc. the frequency of cc genoty ... | 2010 | 20589073 |
| endoscopic full-thickness resection and defect closure in the colon. | endoscopic full-thickness resection (eftr) is a minimally invasive method for en bloc resection of gi lesions. | 2010 | 20598252 |
| seroprevalence and risk factors associated with toxoplasma gondii in domestic pigs from spain. | serum samples from 2970 (1400 sows, 1570 fattening) pigs, from 100 farms in the 10 main swine production regions in spain were tested for antibodies against t. gondii by the modified agglutination test (mat). antibodies to t. gondii (mat 1:25 or higher) were detected in 492 pigs (16.6%, 9.7% in fattening pigs and 24.2% in sows). the herd prevalence was 85.0% (95% ci: 78-92) and within-farm prevalence ranged from 2.9% to 92.8% (median=17.6%). statistically significant differences were observed am ... | 2010 | 20601110 |
| different fusion configurations of evolutionarily conserved segments in karyotypes of potamochoerus porcus and phacochoerus africanus. | the karyotype of the red river hog potamochoerus porcus (2n = 34) differs from that of the domestic pig by the presence of 2 fusion chromosomes homologous to pig chromosomes 13/16 and 15/17. moreover, chromosomes corresponding to pig chromosomes 13/16 and 1 are both acrocentric. hybridization with region-specific painting probes confirmed tandem fusion of pig chromosomes 13 and 16, and a pericentric inversion of the pig chromosome 1p equivalent in p. porcus. the chromosome complement of the wart ... | 2010 | 20606389 |
| seeding endothelial progenitor cells on a self-expanding metal stent: an in vitro study. | to demonstrate the feasibility of seeding a self-expanding metal stent with endothelial progenitor cells to enhance rapid reendothelialization, which is postulated to prevent local thrombus formation and restenosis after vascular intervention. | 2010 | 20610181 |
| does pressure matter in creating burns in a porcine model? | multiple animal models of burn injury have been reported, and only some of these have been fully validated. one of the most popular approaches is burn infliction by direct contact with the heat source. previous investigators have reported that the pressure of application of the contact burn infliction device does not affect the depth of injury. we hypothesized that the depth of injury would increase with increasing pressure of application in a porcine burn model. forty mid-dermal contact burns m ... | 2010 | 20616654 |
| a role for factor xiia-mediated factor xi activation in thrombus formation in vivo. | mice lacking factor xii (fxii) or factor xi (fxi) are resistant to experimentally-induced thrombosis, suggesting fxiia activation of fxi contributes to thrombus formation in vivo. it is not clear whether this reaction has relevance for thrombosis in pri mates. in 2 carotid artery injury models (fecl(3) and rose bengal/laser), fxii-deficient mice are more resistant to thrombosis than fxi- or factor ix (fix)-deficient mice, raising the possibility that fxii and fxi function in distinct pathways. a ... | 2010 | 20634381 |
| micrornaome of porcine pre- and postnatal development. | the domestic pig is of enormous agricultural significance and valuable models for many human diseases. information concerning the pig micrornaome (mirnaome) has been long overdue and elucidation of this information will permit an atlas of microrna (mirna) regulation functions and networks to be constructed. here we performed a comprehensive search for porcine mirnas on ten small rna sequencing libraries prepared from a mixture of tissues obtained during the entire pig lifetime, from the fetal pe ... | 2010 | 20634961 |
| establishment of a streptozotocin-induced diabetic domestic pig model and a systematic evaluation of pathological changes in the hard and soft tissue over a 12-month period. | the number of diabetic patients in need of medical treatment is growing steadily. therefore, a diabetic animal model with high degree of similarities with humans, which is suitable for the systematic evaluation of biomaterials and medical devices, is needed. | 2010 | 20636725 |
| protection of the renal collecting system during radiofrequency ablation with antegrade cold dextrose infusion. | to evaluate the feasibility and efficacy of antegrade renal cooling during radiofrequency (rf) ablation to protect the renal collecting system in pigs. | 2010 | 20651062 |
| tetrahydrolipstatin inhibition, functional analyses, and three-dimensional structure of a lipase essential for mycobacterial viability. | the highly complex and unique mycobacterial cell wall is critical to the survival of mycobacteria in host cells. however, the biosynthetic pathways responsible for its synthesis are, in general, incompletely characterized. rv3802c from mycobacterium tuberculosis is a partially characterized phospholipase/thioesterase encoded within a genetic cluster dedicated to the synthesis of core structures of the mycobacterial cell wall, including mycolic acids and arabinogalactan. enzymatic assays performe ... | 2010 | 20656688 |
| the effects of rapid enzymatic debridement of deep partial-thickness burns with debrase on wound reepithelialization in swine. | reepithelialization of deep burns requires spontaneous or active removal or debridement of the necrotic eschar, as recently defined by the american burn association. debrase is a bromelain-derived enzymatic preparation that has been shown to result in rapid and selective debridement of human and animal burns. the authors hypothesized that rapid debridement of deep dermal burns with debrase would result in earlier reepithelialization of the remaining dermis in a porcine model. eighty deep dermal ... | 2010 | 20661148 |
| echo-endoscope biplane navigation system for endovascular aneurysm repair. | requirement of contrast medium limits the indication of endovascular intervention to patients with renal insufficiency and allergic reaction. furthermore, landmarks under fluoroscopic guidance do not necessarily encompass the local anatomy of complex aortic lesions. we evaluated the feasibility of a bimodal monitoring system consisting of echocardiography and endoscopy as a more accurate anatomical navigation system for endovascular intervention. | 2010 | 20667361 |
| a tractable experimental model for study of human and animal scabies. | scabies is a parasitic skin infestation caused by the burrowing mite sarcoptes scabiei. it is common worldwide and spreads rapidly under crowded conditions, such as those found in socially disadvantaged communities of indigenous populations and in developing countries. pruritic scabies lesions facilitate opportunistic bacterial infections, particularly group a streptococci. streptococcal infections cause significant sequelae and the increased community streptococcal burden has led to extreme lev ... | 2010 | 20668508 |
| effects of sodium selenite and amiloride on calvarial calcification in closing small cranial defects. | fresh autogenous bone graft is the most preferred osteoplastic material, whether the purpose is cosmetic, psychological, or for the protection of the brain. these grafts are not rejected and do not react immunologically. the aim of this study was to evaluate the efficacy of autogenous fat rolled with bone dust derived from the bur hole in closing small cranial defects. additionally, the authors examined the morphological and biochemical effects of na selenite and amiloride on calvarial calcifica ... | 2011 | 20672896 |
| chondroitin sulfate demarcates astrocytic territories in the mammalian cerebral cortex. | adjacent astrocytes in the grey matter of the mammalian cerebral cortex are organized in a tile-like manner and separated from one another, forming discrete domains named "non-overlapping territories". we have previously reported that an anti-chondroitin sulfate (cs) antibody, cs-56, marks a subpopulation of cortical astrocytes which we named the dandelion clock-like structure (dacs) based on its morphological characteristics. in the present study, we found that another anti-cs antibody (anti-cs ... | 2010 | 20678547 |
| in vitro permeation of a pegylated naltrexone prodrug across microneedle-treated skin. | microneedles (mn) are a useful tool for increasing skin permeability to xenobiotics. previous research showed marked improvement in the percutaneous flux of naltrexone (ntx) hydrochloride by the use of mn skin pretreatment alone; however, for better therapeutic effect, further enhancement is desired. the goal of this in vitro study was to combine microneedle skin pretreatment with the use of a highly water-soluble pegylated naltrexone prodrug (polyethyleneglycol-ntx, peg-ntx) to investigate its ... | 2010 | 20678989 |
| comparative pathology of select agent influenza a virus infections. | influenza a virus infections may spread rapidly in human populations and cause variable mortality. two of these influenza viruses have been designated as select agents: 1918 h1n1 virus and highly pathogenic avian influenza (hpai) virus. knowledge of the pathology of these virus infections in humans, other naturally infected species, and experimental animals is important to understand the pathogenesis of influenza, to design appropriate models for evaluation of medical countermeasures, and to mak ... | 2010 | 20682805 |
| effect of continuous compressions and 30:2 cardiopulmonary resuscitation on global ventilation/perfusion values during resuscitation in a porcine model. | rescue ventilations during bystander resuscitation, although previously considered essential, interrupt the continuity of chest compressions and might have deleterious effects in basic life support. this study was undertaken to analyze the global ventilation/perfusion values of continuous compressions and 30:2 cardiopulmonary resuscitation to determine the effectiveness for each approach in a porcine model of prolonged bystander cardiopulmonary resuscitation for ventricular fibrillation. | 2010 | 20683258 |
| analysis of 11 tetrameric strs in wild boars for forensic purposes. | str profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety. we tested the efficacy of a multiplex pcr-based assay including 11 porcine-specific tetrameric strs in a population sample of wild boars (n=142) originating from piedmont (north west italy). multiple deviations from hardy-weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative ... | 2010 | 20685189 |
| the rethink project--minipigs as models for the toxicity testing of new medicines and chemicals: an impact assessment. | the objective of the rethink project was to evaluate the potential impact of toxicity testing in the minipig as an alternative approach in regulatory toxicity testing that can contribute to the replacement, refinement and reduction of animal testing (3rs). minipigs are strains of domestic pigs that are markedly smaller than farmyard varieties, and thus better adapted to laboratory housing. the pig closely resembles man in many features of its anatomy, physiology, biochemistry and lifestyle. in p ... | 2010 | 20685395 |
| experimental study of pancreaticojejunostomy completed using anastomotic chains. | the most difficult, time-consuming, and complication-prone step in pancreaticoduodenectomy is the pancreaticojejunostomy step. the largest disadvantage of this kind of anastomosis is the high incidence of postoperative anastomotic leakage. once pancreatic leakage occurs, the patient death rate can be very high. the aim of this study was to design a pancreaticojejunostomy procedure using anastomotic chains, which results in the cut end of the jejunum being attached to the pancreatic stump without ... | 2010 | 20687743 |
| prevalence of actinobacillus pleuropneumoniae infection in hunted wild boars (sus scrofa) in germany. | actinobacillus pleuropneumoniae is an important pathogen of the porcine respiratory tract. subclinically and chronically infected pigs may enter a permanent carrier state, harboring a. pleuropneumoniae in lungs or tonsils. despite the enormous role of a. pleuropneumoniae in domestic swine production, no published data about prevalence and distribution of a. pleuropneumoniae infections in wild boars (sus scrofa) are available. antibodies against a. pleuropneumoniae have been detected in serum of ... | 2010 | 20688648 |
| a three-dimensional digital segmented and deformable brain atlas of the domestic pig. | we used high-magnetic field (4.7 t) magnetic resonance imaging (mri) to build the first high-resolution (100 microm x 150 microm x 100 microm) three-dimensional (3d) digital atlas in stereotaxic coordinates of the brain of a female domestic pig (sus scrofa domesticus). this atlas was constructed from one hemisphere which underwent a symmetrical transformation through the midsagittal plane. concomitant construction of a 3d histological atlas based on the same scheme facilitated control of deep br ... | 2010 | 20692291 |
| examination of protective effect of ischemic postconditioning after small bowel autotransplantation. | ischemia/reperfusion (i/r) injury is a serious condition that results from some surgical procedures, including intestinal transplantation. ischemic postconditioning is defined as brief periods of reperfusion alternating with reocclusion applied during the early minutes after reperfusion. the objective of this study was to investigate the effect of ischemic postconditioning before small bowel autotransplantation. total orthotopic intestinal autotransplantation was performed in 30 white domestic p ... | 2010 | 20692464 |
| durability of the self-approximating translumenal access technique (stat) for potential use in natural orifice translumenal surgery (notes). | the self-approximating translumenal access technique (stat) has been shown to provide a safe and reliable means of abdominal access for natural orifice translumenal endoscopic surgery (notes). however, the feasibility of using stat for translumenal organ resection is unknown. this study aimed to evaluate the technical performance of organ resection using stat, the integrity of the stat gastric tunnel after organ resection, and the postoperative morbidity of organ resection using stat. | 2011 | 20725749 |
| toxicity of valproic acid in liver slices from sprague-dawley rats and domestic pigs. | the reason for sensitivity to valproic acid (vpa) hepatotoxicity in humans is not known and requires further investigation. we investigated two in vitro animal models that might represent the unpredictably sensitive and the predictably non-sensitive populations of patients. vpa-induced hepatotoxicity was evaluated in vitro using precision-cut liver slices prepared from adult sprague-dawley rats and 4-wk-old domestic pigs. protein synthesis, k(+) retention and cytosolic lactate dehydrogenase leak ... | 1991 | 20732016 |
| camp-activated ca2+ signaling is required for cftr-mediated serous cell fluid secretion in porcine and human airways. | cystic fibrosis (cf), which is caused by mutations in cftr, affects many tissues, including the lung. submucosal gland serous acinar cells are primary sites of fluid secretion and cftr expression in the lung. absence of cftr in these cells may contribute to cf lung pathogenesis by disrupting fluid secretion. here, we have isolated primary serous acinar cells from wild-type and cftr-/- pigs and humans without cf to investigate the cellular mechanisms and regulation of fluid secretion by optical i ... | 2010 | 20739756 |
| a study on repair of porcine articular cartilage defects with tissue-engineered cartilage constructed in vivo by composite scaffold materials. | the study was performed to find out a promising injectable composite scaffold for cartilage tissue engineering. by using a composite of allogenous cartilage microparticle acellular tissue matrix (cmactm) and fibrin glue (fg) as injectable scaffold materials, tissue-engineered cartilage was constructed in vivo, and the effects of which on the repair of porcine articular cartilage defects were observed. cmactm was obtained from domestic pigs. the chondrocytes were prepared from experimental mini-t ... | 2010 | 20798625 |
| peptide vaccine candidates against classical swine fever virus: t cell and neutralizing antibody responses of dendrimers displaying e2 and ns2-3 epitopes. | three peptide-based systems integrating b and t antigenic sites of csfv and displaying the b epitopes in fourfold presentation have been designed and produced, and shown to bring about significant enhancements in immunogenicity over the peptides in monomeric form. of the different strategies tested for producing the dendrimeric constructs, stepwise spps using 3,6-dioxaoctanoic acid as flexible, peg-like spacer units at the branching points is clearly advantageous, in particular over ligation in ... | 2011 | 20812373 |
| new piophilid in town: the first palaearctic record of piophila megastigmata and its coexistence with piophila casei in central spain. | the genus piophila fallén (diptera: piophilidae) is known from only two species: piophila casei (l.) is a major pest, a cosmopolitan species and is commonly used as a forensic indicator, whereas piophila megastigmata mcalpine has until now only been recorded in a natural environment in south africa. the present work reports the first occurrence of p. megastigmata in the palaearctic region from specimens collected by carrion-baited traps throughout different natural habitats of central spain. fur ... | 2011 | 20819151 |
| volumetric tracking tool using four-dimensional ct for image guided-radiation therapy. | to analyze lung vessel motion automatically, we developed computerized tracking software for multi-slice ct (msct) data. here, we evaluated the tracking accuracy in images of healthy porcine lungs obtained by 256 multi-slice ct (256-msct). a domestic pig was scanned with 256-msct under free breathing conditions. the 256-msct provides 128-mm scan coverage with a 0.5-mm slice thickness in a single rotation (1.0 s/rot). automatic tracking was performed by template matching, where volumes of interes ... | 2008 | 20821162 |
| reassortant between human-like h3n2 and avian h5 subtype influenza a viruses in pigs: a potential public health risk. | human-like h3n2 influenza viruses have repeatedly been transmitted to domestic pigs in different regions of the world, but it is still uncertain whether any of these variants could become established in pig populations. the fact that different subtypes of influenza viruses have been detected in pigs makes them an ideal candidate for the genesis of a possible reassortant virus with both human and avian origins. however, the determination of whether pigs can act as a "mixing vessel" for a possible ... | 2010 | 20830295 |
| establishment of a porcine model for lobar lung auto-transplantation. | the aim of this study was to develop a porcine model of left single lung auto-transplantation. | 2010 | 20832588 |
| contrast-enhanced ultrasound for blunt hepatic trauma: an animal experiment. | the aim of this study was to study the value of contrast-enhanced ultrasound (ceus) in the diagnosis of active hemorrhage and intraparenchymal lesions in blunt hepatic trauma versus conventional ultrasound (us). | 2010 | 20837263 |
| video: transcervical videoscopic esophageal dissection in minimally invasive esophagectomy. | minimally invasive esophagectomy (mie) may involve video-assisted thoracoscopic surgery (vats) for mediastinal esophageal dissection. usually, vats requires single-lung ventilation and has associated cardiopulmonary morbidity [1-3]. alternatively, transhiatal dissection can be performed, although its complications include vocal cord palsy [4], cardiac arrythmias [5], and increased bleeding [5, 6], the latter associated with mortality after esophagectomy [2]. therefore, the feasibility of mie usi ... | 2011 | 20844900 |