| gaba-ergic and glycinergic pathways in the inner plexiform layer of the goldfish retina. | gaba-ergic and glycinergic circuitry in the inner plexiform layer of the goldfish retina was evaluated by electron microscopic autoradiography of 3h-gaba and 3h-glycine uptake, combined with retrograde horseradish peroxidase (hrp) labeling of ganglion cells. gaba-ergic and glycinergic synapses were found on labeled ganglion cells throughout the inner plexiform layer. this reinforces the idea that physiological evidence of gaba-ergic and glycinergic influence on a variety of ganglion cells in gol ... | 1990 | 2298935 |
| motor innervation of respiratory muscles and an opercular display muscle in siamese fighting fish betta splendens. | horseradish peroxidase was used to identify motor neurons projecting to the adductor mandibulae, levator hyomandibulae, levator operculi, adductor operculi, and dilator operculi muscles in siamese fighting fish, betta splendens. these muscles participate in the production of respiratory and feeding movements in teleost fishes. the dilator operculi is also the effector muscle for gill-cover erection behavior that is part of betta's aggressive display. the motor innervation of these muscles in bet ... | 1989 | 2592620 |
| morphology of physiologically identified mitral cells in the carp olfactory bulb: a light microscopic study after intracellular staining with horseradish peroxidase. | physiologically identified mitral cells in the carp olfactory bulb were stained by intracellular injection of horseradish peroxidase in order to study the morphology in detail. the somata were fusiform, elongated, oval, triangular, or irregular. the mean diameters of the somata were 30 microns x 14 microns. two to five thick dendrites arose from the somata and frequently gave off branches to form glomerular tufts. the dendrites extended less than 400 microns; the dendritic field of single mitral ... | 1988 | 3343400 |
| a computer-assisted study of quantitative morphological features of c type horizontal cells in retina. | biphasic c type horizontal cells of the retina of crucian carp were intracellularly stained with horseradish peroxidase (hrp) and quantitatively studied with computer-aided three-dimensional reconstruction techniques. several parameters characterizing their morphology were determined and compared among different subtypes of horizontal cells. the results show that both cell body area and horizontal extension are proper parameters by which different subtypes of biphasic horizontal cells can be cle ... | 1987 | 3659894 |
| telencephalic afferent nuclei in the carp diencephalon, with special reference to fiber connections of the nucleus preglomerulosus pars lateralis. | fiber connections of the nucleus preglomerulosus pars lateralis (pgl), which primarily provides afferent inputs to the telencephalon, were examined in carp by means of horseradish peroxidase tracing methods. the major afferent sources of pgl are the bilateral nucleus tuberis anterior and a few projections are found deriving from the ipsilateral nucleus ventromedialis thalami, nucleus centralis posterior, dorsal periventricular hypothalamus, and torus semicircularis. axons arising in the pgl can ... | 1986 | 2429725 |
| gap-junctional contacts of luminosity-type horizontal cells in the carp retina: a novel pathway of signal conduction from the cell body to the axon terminal. | l-type horizontal cells (271) of the carp retina were studied with the aid of intracellular injections of lucifer yellow and horseradish peroxidase (hrp). coupling among these cells was revealed by passage of the dye out of the injected cell. thick processes that crossed axons filled with lucifer yellow also contained the stain. these thick processes appear to be the axon terminals of other horizontal cells. serial reconstruction of an axon terminal by electron microscopy revealed two groups of ... | 1986 | 3734163 |
| the time-course of appearance and net accumulation of horseradish peroxidase (hrp) presented orally to juvenile carp cyprinus carpio (l.). | a sensitive enzyme-linked immunosorbent assay (elisa) technique was used in order to determine horseradish peroxidase (hrp) uptake from the carp gut. hrp was detected in blood plasma and various tissues within 15 min of oral intubation. the time-course of net accumulation (uptake-degradation) over a 2 hr period was recorded. the presence of hrp reached a maximum in the body tissues approximately 1 hr after intubation and on a microgram/g wet weight basis the order of accumulation within the tiss ... | 1986 | 2875835 |
| surface markers of male germ cells in early development of carp (cyprinus carpio l.) and the blood-testis barrier in fish. a study with monoclonal antibodies and horseradish peroxidase (hrp). | | 1985 | 4028174 |
| peripheral and central distribution of major branches of the facial taste nerve in the carp. | the major pathways of the peripheral facial taste system in the carp, cyprinus carpio, are the maxillary (max), mandibular (mand), palatine (pal) and recurrent nerve rami. the peripheral distribution of the sensory fibers of these branches (b) was determined by means of electrophysiological techniques. max.b., mand.b. and pal.b., each of which arises from the gasserian-geniculate ganglionic complexes, were found to innervate respectively, the upper lip and the adjacent skin, the internal and ext ... | 1985 | 3978434 |
| uptake and transport of intact macromolecules in the intestinal epithelium of carp (cyprinus carpio l.) and the possible immunological implications. | two protein antigens, horseradish peroxidase (hrp) and ferritin, have been administered to the digestive tract of carp. electron-microscopical observations reveal considerable absorption of both antigens in the second segment of the gut (from 70 to 95% of the total length) and also, although to a lesser extent, in the first segment (from 0 to 70% of the total length). even when administered physiologically with food, a large amount of ferritin is absorbed by enterocytes in the second gut segment ... | 1985 | 3986879 |
| light-dependent change of cone-horizontal cell interactions in carp retina. | carp h2 horizontal cells were investigated at various stages of dark and light adaptation. their state of adaptation was verified by evaluating in computer reconstructed serial sections the number of small, fingerlike protrusions originating from h1 processes. these so-called spinules are absent in the dark-adapted state and are reformed during light adaptation. intracellular recordings showed that long-wave-induced depolarization thought to be mediated by sign-inverted feedback from h1 horizont ... | 1984 | 6326947 |
| connections between photoreceptors and horseradish peroxidase-injected bipolar cells in the carp retina. | we describe two physiologically and morphologically distinct types of on-center bipolar cell in the carp retina. these cells can be distinguished from one another in the following respects: (1) "type i" cells respond to moderate light intensities with a transient depolarization followed by a plateau, while the response of "type ii" cells is approximately rectangular in shape; (2) latency of type ii cell responses is shorter than that of type i cell responses; (3) mean diameter of type ii cell de ... | 1983 | 6880034 |
| anatomy of respiratory rhythmic systems in brain stem and cerebellum of the carp. | the afferent and efferent connections of two respiratory rhythmic loci in the dorsal mesencephalic tegmentum were studied by retrograde and anterograde transport of horseradish peroxidase. the injection areas were determined with extracellular activity recording using hrp filled glass micropipettes, and then followed by electrical stimulation and subsequent iontophoretic hrp delivery. one area in the nucleus of the posterior commissure was found to be optic related respiratory in nature and poss ... | 1981 | 7317791 |
| fine structure and radioautography of retinal cone outer segments in goldfish and carp. | cone but not rod outer segments (oss) in the retinas of goldfish and carp are diffusely labeled after intravitreal injection of 3h-fucose. among the different cone types, the oss are heavily labeled in the long double, short single, and some long single cones. the oss of short double and the remaining long single cones are consistently more lightly labeled. a correlation thus exists between heavy labeling of oss maximally sensitive to red and blue light and lighter labeling of oss with maximum s ... | 1980 | 7390721 |
| proprioceptive reflex connections of head musculature and the mesencephalic trigeminal nucleus in the carp. | the location of the sensory cells concerned with the proprioception of respiratory and extraocular musculature in the carp was studied by retrograde transport of horseradish peroxidase. sensory cell labeling after intramuscular hrp injection was exclusively found in the trigeminal-facial-anterior lateral line ganglion complex. the muscles innervated by the trigeminal system are represented in the more rostral ganglion areas, the muscles innervated by the facial system in the more caudal ganglion ... | 1979 | 762276 |
| displaced ganglion cells in carp retina revealed by the horseradish peroxidase technique. | displaced ganglion cells were identified in the carp retina by the retrograde axonal transport of horseradish peroxidase injected into the optic tectum. cells in the ganglion cell layer were all labelled, while in the amacrine cell layer only 1/4000 cells were labelled. because of such low population, it is unlikely to penetrate displaced ganglion cells by microelectrodes. the present finding suggests that the spiking cells frequently recorded in the inner nuclear layer are genuine amacrine cell ... | 1978 | 19605210 |
| extraocular muscle representation in the brainstem of the carp. | the representation of the extraocular muscles in the oculomotor nuclei in the brainstem of the carp was studied with the horseradish peroxidase technique. some normal anatomical features of the various oculomotor nuclei are described. following intramuscular hrp injections in the eye muscles and applying survival times of up to 25 days labeled perikarya were found in the various nuclei of the oculomotor complex. rectus inferior, rectus internus, and obliquus inferior are represented in the ipsil ... | 1978 | 641230 |
| the connections of the trigeminal and facial motor nuclei in the brain of the carp (cyprinus carpio l.) as revealed by anterograde and retrograde transport of horseradish peroxidase. | the connections of the rostral and caudal parts of the trigeminal and facial motor nuclei in the carp were studied with the horseradish peroxidase technique. following ionophoretic peroxidase injections in these motor nuclei, retrogradely labeled cells were observed together with anterogradely labeled motor cell processes. several cellular areas in thalamus, cerebellum and medulla oblongata were shown to project to the v and vii motor nuclei. labeled cells were found in the inferior lobe and the ... | 1977 | 903419 |
| a somatotopic and functional representation of the respiratory muscles in the trigeminal and facial motor nuclei of the carp (cyprinus carpio l.). | the place of the cells innervating six most important respiratory muscles in the carp (cyprinus carpio l.) were determined by means of the horseradish peroxidase technique. it was found that these muscles are represented in the rostral and caudal subdivisions of the trigeminal and facial motor nuclei. all connections were ipsilateral. the distribution of the various cell populations over the different subnuclei has been related to the function of the respective muscles in the respiratory cycle. ... | 1976 | 1262554 |
| close association between paralogous multiple isomirs and paralogous/orthologues mirna sequences implicates dominant sequence selection across various animal species. | micrornas (mirnas) are crucial negative regulators of gene expression at the post-transcriptional level. next-generation sequencing technologies have identified a series of mirna variants (named isomirs). in this study, paralogous isomir assemblies (from the mirna locus) were systematically analyzed based on data acquired from deep sequencing data sets. evolutionary analysis of paralogous (members in mirna gene family in a specific species) and orthologues (across different animal species) mirna ... | 2013 | 23856130 |
| effects of invasive fish and quality of water and sediment on macrophytes biomass, and their consequences for the waterbird community of a mediterranean floodplain. | floodplains are among the most threatened ecosystems world-wide because of multiple stressors, i.e., invasive species, pollution and aquifer overexploitation; the tablas de daimiel national park (spain) is a clear example of these kinds of impact. this work aims to test whether invasive fish and/or the water and sediment quality are significant drivers of the decline of stonewort (chara spp.) meadows in the tablas de daimiel, investigating how this could explain changes observed in the waterbird ... | 2016 | 26896580 |
| azadirachtin, a neem-derived biopesticide, impairs behavioral and hematological parameters in carp (cyprinus carpio). | azadirachtin (aza) is a promisor biopesticide used in organic production and aquaculture. although this compound is apparently safe, there is evidence that it may have deleterious effects on fish. behavioral and hematological tests are grouped into a set of parameters that may predict potential toxicity of chemical compounds. here, we investigate the effects of aza, in the commercial formulation neenmax(™) , on carp (cyprinus carpio) by defining lc50 (96 h), and testing behavioral and hematologi ... | 2016 | 25847134 |
| in vitro antioxidant and hepatoprotective potential of azolla microphylla phytochemically synthesized gold nanoparticles on acetaminophen - induced hepatocyte damage in cyprinus carpio l. | the present study aims to evaluate the hepatoprotective and antioxidant effects of gold nanoparticles (gnap) biosynthesized through the mediation of azolla microphylla and a. microphylla extract on acetaminophen-induced hepatocyte damage in common carp fish (cyprinus carpio l.). the gold nanoparticles (100, 150, 200 μg/ml) and a. microphylla extract powder (100, 200, 400 μg/ml) were added to the primary hepatocytes in different conditions: treatment i (before 12 mm acetaminophen), treatment ii ( ... | 2015 | 25862331 |
| effect of diphenyl diselenide diet supplementation on oxidative stress biomarkers in two species of freshwater fish exposed to the insecticide fipronil. | the ability of diphenyl diselenide [(phse)2] to attenuate oxidative damage was evaluated in the liver, gills, brain, and muscle of carp (cyprinus carpio) and silver catfish (rhamdia quelen) experimentally exposed to fipronil (fpn). initially, the fish were fed a diet without (phse)2 or a diet containing 3.0 mg/kg of (phse)2 for 60 days. after the 60-day period, the fish were exposed to 0.65 µg/l of fpn for 192 h. the results showed that carp exposed to fpn and not fed with (phse)2 exhibited acet ... | 2016 | 27048596 |
| integrated assessment of biomarker response in carp (cyprinus carpio) and silver catfish (rhamdia quelen) exposed to clomazone. | clomazone is considered a potential contaminant of groundwater and is persistent in the environment. to verify the effects of clomazone in cyprinus carpio and rhamdia quelen, a method that combines biomarker responses into an index of "integrated biomarker response" (ibr) was used for observed biological alterations in these species. thiobarbituric acid-reactive substances in liver of carp and silver catfish decreased at both concentrations tested. however, in muscle it increased in carp at 3 mg ... | 2015 | 25779373 |
| toxic effects of penoxsulam herbicide in two fish species reared in southern brazil. | toxic effects of penoxsulam herbicide on acetylcholinesterase, thiobarbituric acid-reactive substances and protein carbonyl were studied in silver catfish (rhamdia sp.) and carp (cyprinus carpio). acetylcholinesterase activity was inhibited in both brain and muscle tissue, with the inhibition being greater in carp than in silver catfish. the levels of malondialdehyde (mda), an indicator of lipid peroxidation, decreased in silver catfish brain tissue, but increased in the carp brain. mda also inc ... | 2014 | 24189995 |
| comparative study on effects of dietary with diphenyl diselenide on oxidative stress in carp (cyprinus carpio) and silver catfish (rhamdia sp.) exposed to herbicide clomazone. | the study investigated the capacity of diphenyl diselenide [(phse)2] (3.0mg/kg), on reduce the oxidative damage in liver, gills and muscle of carp and silver catfish exposed to clomazone (192h). silver catfish exposed to clomazone showed increased thiobarbituric acid-reactive substance (tbars) in liver and muscle and protein carbonyl in liver and gills. furthermore, clomazone in silver catfish decrease non-protein thiols (npsh) in liver and gills and glutathione peroxidase and ascorbic acid in l ... | 2013 | 23892285 |
| cloning of a chub metallothionein cdna and development of competitive rt-pcr of chub metallothionein mrna as a potential biomarker of heavy metal exposure. | metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. we sequenced chub (leuciscus cephalus) metallothionein cdna which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. we then used the extended primer method to develop an accurate quantitative competitive rt-pcr assay for metallothionein mrna. rt-pcr was used to measure metallothionein mrna in feral chub from a ra ... | 2015 | 15178096 |
| dietary fish oil replacement by linseed oil: effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (puntius gonionotus) fingerlings. | silver barb (puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in asia. this study in silver barb was carried out to evaluate the effects of total or partial substitution of dietary fish oil (fo) with linseed oil (lo) on growth, nutrient utilization, whole-body composition, muscle and liver fatty acid composition. fish (12.1±0.4g of initial body weight) were fed for 60days with five experimental iso-proteinous, iso-lipidic and iso-caloric diets in which ... | 2017 | 27913275 |
| trans- and cis-urocanic acid, biogenic amine and amino acid contents in ikan pekasam (fermented fish) produced from javanese carp (puntius gonionotus) and black tilapia (oreochromis mossambicus). | ikan pekasam is a fermented fish product produced in malaysia and is usually made from freshwater fish with ground roasted uncooked rice as the main source of carbohydrate. in this study, the amino acid, biogenic amine, and trans- and cis-urocanic acid (uca) contents of fifteen commercial samples of ikan pekasam made from javanese carp and black tilapia, that had undergone either natural or acid-assisted fermentation, were quantified. the latter includes either tamarind (tamarindus indica) pulp ... | 2015 | 25442635 |
| physical changes in specimens of five species of cyprinidae preserved in ethanol and frozen. | preservation in 30% ethanol and freezing to a temperature of -20 ± 2° c is an appropriate method for measurement of fish eggs, larvae and juveniles. egg diameter of the common carp cyprinus carpio increased insignificantly by 1·32% after preservation compared with live size. the total length (lt ) of 1 day post-hatching (dph) larvae as well as the standard length (ls) of 16 dph larvae of c. carpio increased significantly (2·95 and 1·50%, respectively) after preservation. egg diameter as well as ... | 2014 | 25041177 |
| a novel functional class i lineage in zebrafish (danio rerio), carp (cyprinus carpio), and large barbus (barbus intermedius) showing an unusual conservation of the peptide binding domains. | species from all major jawed vertebrate taxa possess linked polymorphic class i and ii genes located in an mhc. the bony fish are exceptional with class i and ii genes located on different linkage groups. zebrafish (danio rerio), common carp (cyprinus carpio), and barbus (barbus intermedius) represent highly divergent cyprinid genera. the genera danio and cyprinus diverged 50 million years ago, while cyprinus and barbus separated 30 million years ago. in this study, we report the first complete ... | 2002 | 12165519 |
| [histological research on the endocrine pancreas and the intramural nerve ganglia in the carp (barbus meridionalis petenyi heck)]. | | 1965 | 14342419 |
| chemometric approach to evaluate element distribution in muscle, liver and fish bone of roach (rutilus rutilus), silver bream (blicca bjoerkna) and crucian carp (carassius carassius) from swarzędzkie lake (poland) using icp-ms and fias-cvaas techniques. | the content of elements in fish tissues and organs from swarzędzkie lake was investigated in order to evaluate the possible risk associated with their consumption by animals as well as humans. samples of muscle, liver and fish bone of three fish species; roach (rutilus rutilus), silver bream (blicca bjoerkna) and crucian carp (carassius carassius) were collected from seine catches undertaken as part of the biomanipulation of swarzędzkie lake. element concentration (al, as, cd, co, cr, cu, hg, ni ... | 2016 | 27439756 |
| bioaccumulation of six pcb indicator congeners in a heavily polluted water reservoir in eastern slovakia: tissue-specific distribution in fish and their parasites. | concentrations of six indicator pcb congeners (iupac nos. 28, 52, 101, 138, 153, and 180) were measured in several organs and adipose tissue of a freshwater predatory fishes (european perch, northern pike, pike perch, wels catfish) as well as in nonpredators (common carp, freshwater bream, goldfish, white bream) and in acanthocephalan acanthocephalus lucii from the water reservoir zemplínska šírava (eastern slovakia), which is considered to be one of the most pcb-contaminated places in europe. c ... | 2012 | 22526286 |
| male primer endocrine responses to preovulatory female cyprinids under natural conditions in sweden. | this study investigated two related aspects of male-female reproductive interactions in the family cyprinidae: (1) whether ovulating female rudd scardinius erythrophthalmus (subfamily leuciscinae) induce endocrine and gonadal priming responses in conspecific males, a phenomenon which has been described only in species from the subfamily cyprininae such as goldfish, carassius auratus, crucian carp carassius carassius and common carp, cyprinus carpio and (2) whether the stimuli mediating these res ... | 2012 | 22220895 |
| [the effect of the accident at the chernobyl atomic electric power station in 1986 on the fish population of a cooling pond]. | the cytogenetic analysis of cyprinus carpio eyes, developing blicca bjoerkna l. eggs, and eggs and larvae of hypophtalmichthys molitrix val. has demonstrated that the yield of cells with chromosome aberrations in the fish species under study is normal, while the level of variability of morphometric indices in offspring is considerably higher than that in parent fish. | 2006 | 1745759 |
| polymorphism of a, b and c loci of lactate dehydrogenase in european fish species of the cyprinidae family. | in 24 fish species of the cyprinidae family, belonging to 21 genera, isoenzyme patterns of lactate dehydrogenase (ldh) were determined, which could be classified in the majority of cases into 3 main groups. isoenzyme patterns in natural hybrids of roach and rudd, roach and bream, roach and bleak were also analysed. in bitterling, polymorphism was observed in b locus of ldh. in white bream polymorphism exists in the a locus. in bream, rudd, silver carp and barbel polymorphism was found in c loci. ... | 1978 | 751530 |
| fibroin-like substance is a major component of the outer layer of fertilization envelope via which carp egg adheres to the substratum. | seven cdna encoding silkworm fibroin homologues were cloned from a carp ovarian cdna library. the encoded proteins are denoted as carp ovarian fibroin-like substances (fls). fls contain a repetitive domain consisting of tandem repeats of dipeptide of gly-x, where x may be any amino acid. each fls has its own unique repeating sequence, such as gqgagqgs, gqgmgqgm, grgqgeghgs, and gfgfgqgs, indicating a family of fls genes exists in carp. fls is exclusively expressed in oocytes and is stored in cor ... | 2002 | 12112605 |
| [fluorescyanine (ichthyopterin) obtained from eggs of bombyx mori and carp scales]. | | 1951 | 14859632 |
| production of fertile sperm from in vitro propagating enriched spermatogonial stem cells of farmed catfish, clarias batrachus. | spermatogenesis is a highly co-ordinated and complex process. in vitro propagation of spermatogonial stem cells (sscs) could provide an avenue in which to undertake in vivo studies of spermatogenesis. very little information is known about the ssc biology of teleosts. in this study, collagenase-treated testicular cells of farmed catfish (clarias batrachus, popularly known as magur) were purified by ficoll gradient centrifugation followed by magnetic activated cell sorting using thy1.2 (cd90.2) a ... | 2016 | 27417669 |
| the effect of silver carp skin-derived peptides on the activities of vegf and hsp70.1 gene transcription promoters. | studies conducted on human cell culture models have demonstrated that collagen-derived peptides can exert a beneficial effect in medicine. however, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. currently attempts are being made to replace animal collagen with fish collagen. | 2016 | 27629728 |
| a silver carp skin derived collagen in bone defect treatment-a histological study in a rat model. | in recent years, there has been increasing interest in elaboration of novel therapeutic strategies, such as the use of the marine collagen products. biochemical properties of marine collagen are different from those of mammalian collagen; e.g., its extremely high solubility in diluted acid. extracts produced using low temperature techniques contain a number of small proteins and collagen with preserved triple helix structure. the aim of the study was to evaluate the influence of a new marine pro ... | 2016 | 27507153 |
| differential dna methylation analysis without a reference genome. | genome-wide dna methylation mapping uncovers epigenetic changes associated with animal development, environmental adaptation, and species evolution. to address the lack of high-throughput methods for dna methylation analysis in non-model organisms, we developed an integrated approach for studying dna methylation differences independent of a reference genome. experimentally, our method relies on an optimized 96-well protocol for reduced representation bisulfite sequencing (rrbs), which we have va ... | 2015 | 26673328 |
| gut melatonin in vertebrates: chronobiology and physiology. | melatonin, following discovery in the bovine pineal gland, has been detected in several extra-pineal sources including gastrointestinal tract or gut. arylalkylamine n-acetyltransferase (aanat) is the key regulator of its biosynthesis. melatonin in pineal is rhythmically produced with a nocturnal peak in synchronization with environmental light-dark cycle. a recent study on carp reported first that melatonin levels and intensity of a ~23 kda aanat protein in each gut segment also exhibit signific ... | 2015 | 26257705 |
| [hormonal induction of in vitro maturation and ovulation of loach oocytes and obtaining egg cells capable of fertilization and development]. | loach oocytes that have reached a definitive size and are surrounded by follicular envelopes are capable of maturation and ovulating under the effect of 1 μg/ml progesterone in 75% leibovitz medium with 1 g/l sodium bicarbonate or with ph adjustment to 9.0 by 1 n sodium hydroxide. inseminated eggs are developed until the stage of when adding 20% bovine serum to the incubation medium. substitution of the bovine serum with 10-20% loach ovarian fluid or 20% carp ovarian fluid provides more complete ... | 2015 | 26204771 |
| functions of two distinct "prolactin-releasing peptides" evolved from a common ancestral gene. | prolactin-releasing peptide (prrp) is one of the rf-amide peptides and was originally identified in the bovine hypothalamus as a stimulator of prolactin (prl) release. independently, another rf-amide peptide was found in japanese crucian carp and named carassius-rfa (c-rfa), which shows high homology to prrp and stimulates prl secretion in teleost fish. therefore, c-rfa has been recognized as fish prrp. however, recent work has revealed that prrp and c-rfa in non-mammalian vertebrates are encode ... | 2014 | 25426099 |
| in vitro glucuronidation kinetics of deoxynivalenol by human and animal microsomes and recombinant human ugt enzymes. | the mycotoxin deoxynivalenol (don), formed by fusarium species, is one of the most abundant mycotoxins contaminating food and feed worldwide. upon ingestion, the majority of the toxin is excreted by humans and animal species as glucuronide conjugate. first in vitro data indicated that don phase ii metabolism is strongly species dependent. however, kinetic data on the in vitro metabolism as well as investigations on the specific enzymes responsible for don glucuronidation in human are lacking. in ... | 2015 | 24927789 |
| use of ultrasonic spectroscopy and viscosimetry for the characterization of chicken skin collagen in comparison with collagens from other animal tissues. | use of traditional sources of collagen such as pork, bovine, and carp has some limitations. chicken skin can be valuable alternative. in this work collagen was isolated from chicken skin using a modified procedure. molecular properties of chicken collagen were analyzed and compared to collagen from other animal skins. acid-soluble collagen type i was obtained with a yield of 25% and water content around 67%. viscosimetry and ultrasonic spectroscopy were newly used for molecular characterization. ... | 2014 | 24279885 |
| determination of pentachlorophenol residue in meat and fish by gas chromatography-electron capture detection and gas chromatography-mass spectrometry with accelerated solvent extraction. | a novel analytical method, using gas chromatography-electron capture detection (gc-ecd) and gc-mass spectrometry detection (ms), was developed for the qualitative and quantitative measurement of pentachlorophenol in meat and fish. the analyte was extracted by methanol-2% trichloroacetic acid (3/1, v/v) with accelerated solvent extraction (ase). the eluted fraction was evaporated and derivatized with acetic anhydride-pyridine (1/1, v/v) for gc-ecd analysis and gc-ms confirmation. the parameters f ... | 2014 | 23690067 |
| development and characterization of cell line from the gill tissue of catla catla (hamilton, 1822) for toxicological studies. | catla gill cell line (icg) was established from gill tissue of indian major carp (catla catla), a freshwater fish cultivated in india. the cell line was maintained in leibovitz's l-15 supplemented with 10% fetal bovine serum. these cells have been sub-cultured more than 55 passages over a period of 2 years. the icg cell line consists predominantly of epithelial-like cells. the cells were able to grow at a wide range of temperatures from 24°c to 32°c with an optimum temperature of 28°c. the growt ... | 2013 | 23237299 |
| establishment and characterization of permanent cell line from gill tissue of labeo rohita (hamilton) and its application in gene expression and toxicology. | rohu gill cell line (lrg) was established from gill tissue of indian major carp (labeo rohita), a freshwater fish cultivated in india. the cell line was maintained in leibovitz's l-15 supplemented with 10 % foetal bovine serum (fbs). this cell line has been sub-cultured more than 85 passages over a period of 2 years. the lrg cell line consists of both epithelial and fibroblastic-like cells. the cells were able to grow at a wide range of temperatures from 22 to 32 °c, the optimum temperature bein ... | 2013 | 23224722 |
| involvement of pi3 kinase and map kinase in igf-i and insulin-induced ovarian steroidogenesis in common carp cyprinus carpio. | previously, we observed that in vitro steroidogenesis in intact ovarian follicles of common carp cyprinus carpio can alone be induced by recombinant human insulin-like growth factor (igf-i) and bovine insulin (b-insulin) and this induction was gonadotropin-independent. to investigate early signal transduction components involved in this process, the possible role of phosphatidylinositol 3-kinase (pi3 kinase) during ovarian steroidogenesis was examined. igf-i and b-insulin induced testosterone an ... | 2013 | 23073341 |
| development of an es-like cell culture system (resc) from rohu, labeo rohita (ham.). | an embryonic stem (es)-like cell culture system resc from a commercially important freshwater carp, labeo rohita, was developed using blastula stage embryos. the cells were cultured in leibovitz-15 (l-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basic fibroblast growth factor at 28 °c under feeder-free conditions. the es-like cells were characterized by their unique morphology, alkaline phosphatase activity, embryoid body forma ... | 2012 | 22707190 |
| exploring androgen-regulated pathways in teleost fish using transcriptomics and proteomics. | in the environment, there are aquatic pollutants that disrupt androgen signaling in fish. laboratory and field-based experiments have utilized omics technologies to characterize the molecular mechanisms underlying androgen-receptor agonism/antagonism. transcriptomics and proteomics studies with 17β-trenbolone, a growth-promoting pharmaceutical found in water systems surrounding cattle feed lots, and androgens such as 17α-methyltestosterone and 17α-methyldihydrotestosterone, have been conducted i ... | 2012 | 22596056 |
| perspectives on fish gonadotropins and their receptors. | teleosts lack a hypophyseal portal system and hence neurohormones are carried by nerve fibers from the preoptic region to the pituitary. the various cell types in the teleost pituitary are organized in discrete domains. fish possess two gonadotropins (gth) similar to fsh and lh in other vertebrates; they are heterodimeric hormones that consist of a common alpha subunit non-covalently associated with a hormone-specific beta subunit. in recent years the availability of molecular cloning techniques ... | 2010 | 19686749 |
| kinetics of deposition, acute toxicity and bioaccumulation of copper in some freshwater organisms. | experiments with environmentally relevant concentrations of cu in glass aquaria revealed that cu was quickly removed from water. cubic regression of cu concentration against time showed that maximum rate of removal was around 69.34-72.11 h irrespective of treatment. the 96 h lc50 value of cu was respectively 0.18, 0.19 and 0.35 mg/l for fish cyprinus carpio, crustacean diaptomus forbesi and worm branchiura sowerbyi. normalizing the lethal values and plotting them against time it was observed tha ... | 2016 | 27778046 |
| the life cycle of thelohanellus kitauei (myxozoa: myxosporea) infecting common carp (cyprinus carpio) involves aurantiactinomyxon in branchiura sowerbyi. | thelohanellus kitauei is a freshwater myxosporean parasite causing intestinal giant cystic disease of common carp. to clarify the life cycle of t. kitauei, we investigated the oligochaete populations in china and hungary. this study confirms two distinct aurantiactinomyxon morphotypes (aurantiactinomyxon type 1 and aurantiactinomyxon type 2) from branchiura sowerbyi as developmental stages of the life cycle of t. kitauei. the morphological characteristics and dna sequences of these two types are ... | 2016 | 27492197 |
| three actinosporean types (myxozoa) from the oligochaete branchiura sowerbyi in china. | three actinosporean types--raabeia, aurantiactinomyxon, and guyenotia--from the oligochaete branchiura sowerbyi beddard collected from a crucian carp (carassius auratus gibelio) pond are described in this report. compared with the actinospores described previously, the raabeia type presents similar spore shape with the actinospore of myxobolus cultus, except a much longer caudal process, 250.8 μm (217.5-276.3). the aurantiactinomyxon and guyenotia type significantly differ from other actinospore ... | 2013 | 23377120 |
| ecotoxicological assessment of cobalt used as supplement in the diet of common carp cyprinus carpio. | experiments were performed in the laboratory to determine if excess levels of co used as dietary supplement (0.0, 0.05, 0.10 and 1.0%) to enhance growth of the fish cyprinus carpio was safe for aquatic organisms. lethal concentrations of co for tadpole of toad bufo melanostictus (96 h lc(50), 17.2 mg/l), oligochaet worm branchiura sowerbyi (96 h lc(50), 179 mg/l) and crustacean zooplankton diaptomus forbesi (96 h lc(50), 1.5 mg/l) were compared with the concentration of co in the medium leached ... | 2011 | 21858707 |
| acute toxicity of synthetic pyrethroid cypermethrin to some freshwater organisms. | ninety-six hours static bioassays were made in the laboratory to determine acute toxicity of cypermethrin to five non-target freshwater organisms belonging to different taxa and niche. susceptibility of the organisms to cypermethrin was in the order: the crustacean diaptomus forbesi > the aquatic insect ranatra filiformis > the freshwater carp cyprinus carpio > the tadpole larva of the toad bufo melanostictus > the oligochaet worm branchiura sowerbyi. ninety-six hours lc50 values of aqueous cype ... | 2008 | 18058051 |
| impacts of quicklime (cao) on the toxicity of copper (cuso4, 5h2o) to fish and fish food organisms. | static bioassays of 96 h duration were conducted in the laboratory using fry of common carp (cyprinus carpio), adult tubificid worm (branchiura sowerbyi) and adult copepod plankton (cyclops viridis) to determine lc50 values of cu and cao to these organisms and effects of interaction between cu and cao. ninety-six hour lc(50) values of cu to fry of common carp, worm and copepod were found to be 1.40 mgl(-1), 0.08 mgl(-1) and 0.03 mgl(-1) respectively. cao up to 500 mgl(-1) did not produce any mor ... | 2005 | 16168741 |
| 1,1,1-trichloroethane: medium-term toxicity to carp, daphnids, and higher plants. | the toxicity of 1,1,1-trichloroethane to carp (cyprinus carpio) and daphnids (daphnia magna) and the effects of gaseous-phase exposure on the growth of higher plants (sorghum bicolor and brassica napus) were investigated. the test systems were designed to minimize loss of the volatile material during the exposure period. during an exposure period of 14 days, there were no mortalities or other symptoms of toxicity in carp exposed to a mean measured 1,1,1-trichloroethane concentration of 7.7 mg/li ... | 1989 | 2737111 |
| sperm quality and its freezing ability throughout the spawning season in prochilodus lineatus and brycon orbignyanus. | the aim of this study was to determine fresh and frozen sperm quality evaluated over two spawning seasons (2013-2014; 2014-2015) in prochilodus lineatus and brycon orbignyanus. the spawning seasons were divided into two sampling periods: november to december and january to february. males were hand-stripped after carp pituitary treatment. fresh sperm motility rate, velocities (curvilinear = vcl; straight-line = vsl; average path = vap), and the beat cross frequency (bcf) were determined using a ... | 2017 | 28166981 |
| bacterial signatures of "red-operculum" disease in the gut of crucian carp (carassius auratus). | fish gut microbiota play important roles in fish immunity, nutrition, and the adaptation to environmental changes. to date, few studies have focused on the interactions among environmental factors, fish diseases, and gut microbiota compositions. we compared the gut bacterial communities of healthy crucian carps (carassius auratus) with those of individuals affected by "red-operculum" disease and corresponding water and sediment microbiota in four fish farm ponds. distinct gut bacterial communiti ... | 2017 | 28364130 |
| bioconcentration and multi-biomarkers of organic uv filters (bm-dbm and od-paba) in crucian carp. | organic uv filters (ouv-fs) are increasingly used in sunscreens and personal care products. in the present work, the bioconcentration and multi-biomarker effects of butyl methoxydibenzoylmethane (bm-dbm) and ethylhexyl dimethyl p-aminobenzoate (od-paba) were investigated in crucian carp (carassius auratus). the fish were exposed to various concentrations of bm-dbm (3.88, 35.61, 181.85 and 337.15μg/l), od-paba (4.66, 53.83, 264.22 and 459.32μg/l) and their mixture (2.31+2.79, 23.69+26.18, 97.37+1 ... | 2017 | 28343007 |
| influence of multi-walled carbon nanotubes on the effects of roxithromycin in crucian carp (carassius auratus) in the presence of natural organic matter. | carbon nanotubes are increasingly entering the aquatic environment and may interact with other co-existing contaminants, such as antibiotics. however, whether these interactions may affect their bioavailability in aquatic organisms is the subject of considerable debate. the primary objective of this study was to assess the risks arising from the coexistence of roxithromycin (rox) and multi-walled carbon nanotubes (mwcnts) in waters containing natural organic matter (nom), focusing on the distrib ... | 2017 | 28324838 |
| allometric analysis of a morphological anti-predator trait in geographic populations of japanese crucian carp. | costly anti-predator traits tend to be expressed only in high-predation conditions. for the cyprinid fish genus carassius, deeper body depth is more adaptive to avoid predation by gape-limited piscivorous fish, but it raises swimming costs. it is therefore predicted that the relative body depth will decrease when the prey fish has reached a size larger than the predator gape-size. this prediction was tested by allometric analysis of the relation between body depth and standard length of triploid ... | 2017 | 28150742 |
| occurrence of parasites of the genus eustrongylides spp. (nematoda: dioctophymatidae) in fish caught in trasimeno lake, italy. | eustrongylides spp. is considered a freshwater fish zoonotic nematode. in the present study, the prevalence of eustrongylides spp. in six edible fish (european perch - perca fluviatilis, goldfish - carassius auratus, largemouth black bass - micropterus salmoides, tench- tinca tinca, carp - cyprinus carpio and sand smelt - atherina boyeri) of trasimeno lake was surveyed. the investigations were conducted from october 2014 to september 2015 and 384 specimens per species for each season were caught ... | 2016 | 28058245 |
| modulation of erythromycin-induced biochemical responses in crucian carp by ketoconazole. | the individual and combined biochemical responses of erythromycin and ketoconazole have been examined in an organism representative of the aquatic environment, crucian carp (carassius auratus). the possible interactions between erythromycin and ketoconazole were investigated on the bioaccumulation and the expression of biotransformation enzymes 7-ethoxyresorufin-o-deethylase (erod) and glutathione s-transferase (gst), and an antioxidant defense enzyme superoxide dismutase (sod) in fish tissues. ... | 2017 | 28004371 |
| spontaneous polyploidy, gynogenesis and androgenesis in second generation (f2 ) koi cyprinus carpio × goldfish carassius auratus hybrids. | the objective of this study was to characterize the genetics of second generation (f2 ) koi cyprinus carpio × goldfish carassius auratus hybrids. spermatozoa produced by a novel, fertile f1 male were found to be diploid by flow-cytometric analysis. backcross (f1 female × c. carpio male and c. carpio female × f1 male) juveniles were triploid, confirming that female and male f1 hybrids both produced diploid gametes. the vast majority of surviving f2 juveniles was diploid and small proportions were ... | 2017 | 27861861 |
| complete genome sequence and architecture of crucian carp carassius auratus herpesvirus (cahv). | crucian carp carassius auratus herpesvirus (cahv) was isolated from diseased crucian carp with acute gill hemorrhages and high mortality. the cahv genome was sequenced and analyzed. the data showed that it consists of 275,348 bp and contains 150 predicted orfs. the architecture of the cahv genome differs from those of four cyprinid herpesviruses (cyhv1, cyhv2, sy-c1, cyhv3), with insertions, deletions and the absence of a terminal direct repeat. phylogenetic analysis of the dna polymerase sequen ... | 2016 | 27591780 |
| acute ammonia toxicity in crucian carp carassius auratus and effects of taurine on hyperammonemia. | the four experimental groups were carried out to test the response of crucian carp carassius auratus to ammonia toxicity and taurine: group 1 was injected with nacl, group 2 was injected with ammonium acetate, group 3 was injected with ammonium acetate and taurine, and group 4 was injected with taurine. fish in group 2 had the highest ammonia and glutamine contents, and the lowest glutamate content in liver and brain. serum superoxide dismutase (sod), glutathione (gsh) activities, red cell count ... | 2016 | 27510860 |
| effects of inducers of cytochrome p450s on enrofloxacin n-deethylation in crucian carp carassius auratus gibelio. | in this study with crucian carp (carassius auratus gibelio), the effect on enrofloxacin (ef) and its metabolite ciprofloxacin (cf) and on the activity of cytochrome p450 1a (cyp1a) and cytochrome p450 3a (cyp3a) was estimated following the oral administration of rifampicin (rif) (12mg/kg) and β-naphthoflavone (bnf) (12mg/kg), respectively. first, reversed-phase high-performance liquid chromatography (rp-hplc) was used to detect the pharmacokinetics of ef with continual blood sampling. in rif-tre ... | 2016 | 27490210 |
| homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in cyprinidae. | hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. to study the changing patterns of expression accompanying hybridization and polyploidization, we used rna-seq and qrt-pcr to investigate global expression and homoeologue expression in diploid and tetraploid hybrids of carassius auratus red var. (♀) (r) and cyprinus carpio (♂) (c). by comparing the relative expression levels between the hybrids and their parents, we defined the expres ... | 2016 | 27265401 |
| enzyme activity and morphological change in the spleens of crucian carp in the yongcheng coal mine subsidence area, china. | the goal of the current study was to investigate the effects of pollution on aquatic organisms in the yongcheng coal mine subsidence area. crucian carp (carassius auratus) were collected from yongcheng natural fishpond (experimental group) and tianmu lake (control group), and the spleens were isolated for analysis. subsequently, histological changes, dna damage, and antioxidant enzyme activity were assessed. the result showed that there were more vacuoles, widened blood sinus cavities, increased ... | 2016 | 27173263 |
| effects of ifnγ administration on allograft rejection in ginbuna crucian carp. | in vertebrates, the rejection of allografts is primarily accomplished by cell-mediated immunity. we recently identified four ifnγ isoforms with antiviral activity in ginbuna crucian carp, carassius auratus langsdorfii. however, involvement of the ifnγ isoforms in cell-mediated immunity, especially in t cell function remains unknown. here we investigate expression of the ifnγ isoforms and effects of administration of recombinant ifnγ (rgifnγ) isoforms in ginbuna scale allograft rejection. all fou ... | 2016 | 27156851 |
| autotriploid origin of carassius auratus as revealed by chromosomal locus analysis. | in the dongting water system, the carassius auratus (crucian carp) complex is characterized by the coexistence of diploid forms (2n=100, 2ncc) and polyploid forms. chromosomal and karyotypic analyses have suggested that the polyploid c. auratus has a triploid (3n=150, 3ncc) and a tetraploid origin (4n=200), respectively. however, there is a lack of direct genetic evidence to support this conclusion. in this paper, analysis of the 5s rdna chromosomal locus revealed that the 3ncc is of triploid or ... | 2016 | 27084707 |
| variation in susceptibility pattern of fish to argulus siamensis: do immune responses of host play a role? | branchiuran ectoparasites of the genus argulus can have extensive damaging effects on cultured fish. there exist no systematic studies that evaluate susceptibility or resistance of various carp species to argulus sp. and the underlying mechanisms. the present study aimed at identifying the most susceptible and resistant cultured species, studying settlement and survival of parasite on these species, and finally unravelling the variations of immune response in both resistant and susceptible speci ... | 2016 | 27084477 |
| molecular characterization and expressing analysis of the c-type and g-type lysozymes in qihe crucian carp carassius auratus. | lysozyme as an important nonspecific immune factor, can kill bacteria by hydrolyzing β-1,4-glycosidic linkages of peptidoglycan layer, and plays an important role in innate immune response against pathogen infection. in the present study, we report molecular cloning, tissue distribution and functional characterization of the c-type and g-type lysozymes in qihe crucian carp carassius auratus (designated as ca-clys and ca-glys, respectively). the full-length of ca-clys and ca-glys cdna were cloned ... | 2016 | 27012394 |
| [evaluation of ivermectin's reproductive toxicity to male carassius auratus]. | as a new type of antiparasitic drugs, ivermectin (ivm) has been widely applied in agriculture, stock raising and aquaculture in china because of its broad spectrum and high efficiency. in order to evaluate the ivm' s reproductive toxicity to male crucian carp (carassius auratus), ivm was orally given to the experimental fish with different dosages and the gonadosomatic index (gsi), sexual hormone contents (including testosterone and estradiol) in serum and testis, γ-aminobutyric acid content in ... | 2015 | 26995928 |
| identification, cdna cloning, and characterization of luteinizing hormone beta subunit (lhb) gene in catla catla. | reproductive hormones play a significant role in the gonadal development and gametogenesis process of animals. in the present study luteinizing hormone beta, (lhb) subunit gene was cloned and characterized from the brain of catla catla. the lhb full-length of cdna sequence is 629 bp which consists of 43bp 5'-utr (untranslated region) 447bp, orf(open reading frame) and 139 bp of 3'-utr respectively. the coding region of lhb gene encoded a peptide of 148 amino acids. the coding sequence of lhb gen ... | 2016 | 26980432 |
| phenolic endocrine disrupting chemicals in an urban receiving river (panlong river) of yunnan-guizhou plateau: occurrence, bioaccumulation and sources. | the objectives of this study were to track the occurrence, bioaccumulation and sources of phenolic endocrine disrupting chemicals (edcs) in a representative urban river (panlong river) of yunnan-guizhou plateau. it provided more comprehensive fundamental data for risk assessment and contamination control of phenolic edcs in aquatic environments. phenolic edcs, such as nonylphenol-di-ethoxylate (np2eo), nonylphenol-mono-ethoxylate (np1eo), 4-nonylphenol (4-np), bisphenol a (bpa), 4-cumylphenol (4 ... | 2016 | 26921547 |
| influence of submarine groundwater discharge on (210)po and (210)pb bioaccumulation in fish tissues. | this study presents the results of the accumulation of (210)po and (210)pb in fish tissues and organs in a brackish-water marshland that is characterized by high concentrations of (222)rn and (226)ra supplied by submarine groundwater discharge (sgd). tissues and organs from cyprinus carpio, chelon labrosus and carassius auratus in the wetland were significantly enriched by both (210)pb and (210)po (up to 55 and 66 times, respectively) compared to blanks. the major input route of (210)pb and (210 ... | 2016 | 26913976 |
| bioconcentration and metabolism of ketoconazole and effects on multi-biomarkers in crucian carp (carassius auratus). | the tissue distribution, bioconcentration, metabolism and biological effects of the antifungal medication ketoconazole were investigated in fish, crucian carp (carassius auratus) were exposed to a series of nominal concentrations (0.2, 2 and 20 μg/l) for 14 days. the ultra-high performance liquid chromatography tandem triple quadrupole mass spectroscopy (uplc/ms/ms) analysis was used to determine the bioconcentration of ketoconazole and its metabolites in fish. the highest tissue concentration o ... | 2016 | 26901470 |
| isolation and characterization of hematopoietic stem cells in teleost fish. | despite 400 million years of evolutionary divergence, hematopoiesis is highly conserved between mammals and teleost fish. all types of mature blood cells including the erythroid, myeloid, and lymphoid lineages show a high degree of similarity to their mammalian counterparts at the morphological and molecular level. hematopoietic stem cells (hscs) are cells that are capable of self-renewal and differentiating into all hematopoietic lineages over the lifetime of an organism. the study of hscs has ... | 2016 | 26801099 |
| interactive effects of selected pharmaceutical mixtures on bioaccumulation and biochemical status in crucian carp (carassius auratus). | the aim of this study was to evaluate the interactive effects of fluoxetine (flu), roxithromycin (rox) and propranolol (prp) on the bioaccumulation and biochemical responses in the crucian carp carassius auratus. after 7 days of binary exposure (rox + flu and prp + flu), the addition of waterborne flu at nominal concentrations of 4, 20 and 100 μg l(-1) significantly increased the accumulation of rox and prp in fish livers in most cases, although elevated rox and prp bioaccumulation levels were n ... | 2016 | 26800487 |
| the complete mitochondrial genome of carassius auratus var. high back crucian carp (cypriniformes: cyprinidae). | in this study, the complete mitochondrial genome sequence of carassius auratus var. high back crucian carp (c. auratus var.) was determined (genbank accession number is kt756205). the mitochondrial genome sequence of c. auratus var. was a circular molecule with 16 581 bp in length, and contained 37 typical animal mitochondrial genes, including 2 ribosomal rna genes, 13 protein-coding genes, 22 transfer rna genes and a control region (d-loop). four nucleotide compositions and their relative propo ... | 2017 | 26709972 |
| prevalence of argulus foliaceus in ornamental fishes [goldfish (carassius auratus) and koi (cyprinus carpio)] in kerman, southeast of iran. | the genus argulus (crustacea: branchiura), or fish louse, are common parasites of freshwater fish. this parasite have a direct life cycles and mature females leave the host and lay several hundred eggs on vegetation and various objects in the water. it caused pathological changes due to direct tissue damage and secondary infections. besides the damage and stress caused by argulus itself, one of the main worries for fish producers are the associated secondary infestations and infections that can ... | 2015 | 26688652 |
| comparative proteomic, physiological, morphological, and biochemical analyses reveal the characteristics of the diploid spermatozoa of allotetraploid hybrids of red crucian carp (carassius auratus) and common carp (cyprinus carpio). | the generation of diploid spermatozoa is essential for the continuity of tetraploid lineages. the dna content of diploid spermatozoa from allotetraploid hybrids of red crucian carp and common carp was nearly twice as great as that of haploid spermatozoa from common carp, and the durations of rapid and slow progressive motility were longer. we performed comparative proteomic analyses to measure variations in protein composition between diploid and haploid spermatozoa. using two-dimensional electr ... | 2016 | 26674567 |
| bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (carassius auratus). | diphenhydramine (dph), an antihistamine used to alleviate human allergies, is widespread in aquatic environments. however, little is known about the biochemical and behavioral effects of dph on non-target aquatic animals. in the present study, the tissue distribution, bioconcentration, metabolism, biochemical and behavioral effects were investigated in crucian carp (carassius auratus) exposed to various concentrations of dph (0.84, 4.23, 21.7 and 112 μg l(-1)) for 7d. dph can accumulate in cruci ... | 2016 | 26657385 |
| the chromosomal constitution of fish hybrid lineage revealed by 5s rdna fish. | the establishment of the bisexual fertile fish hybrid lineage including the allodiploid and allotetraploid hybrids, from interspecific hybridization of red crucian carp (carassius auratus red var. 2n = 100, 2n = aa) (♀) × common carp (cyprinus carpio l. 2n = 100, 2n = bb) (♂), provided a good platform to investigate genetic relationship between the parents and their hybrid progenies. | 2015 | 26635010 |
| pharmacokinetics of sarafloxacin in allogynogenetic silver crucian carp, carassius auratus gibelio. | the pharmacokinetic properties of sarafloxacin were investigated after single intravenous (i.v.) and oral (p.o.) administration of 10 mg/kg body weight (b.w.) in allogynogenetic silver crucian carp at 24-26 °c. the plasma concentrations of sarafloxacin were determined by high-performance liquid chromatography. after i.v. administration, the plasma concentration-time data were described by an open two-compartment model. the elimination half-life (t(1/2β)) was estimated to be 22.58 h. the volume o ... | 2016 | 26563280 |
| goldfish (carassius auratus l.) as a model system to study the growth factors, receptors and transcription factors that govern myelopoiesis in fish. | the process of myeloid cell development (myelopoiesis) in fish has mainly been studied in three cyprinid species: zebrafish (danio rerio), ginbuna carp (carassius auratus langsdorfii) and goldfish (c. auratus, l.). our studies on goldfish myelopoiesis have utilized in vitro generated primary kidney macrophage (pkm) cultures and isolated primary kidney neutrophils (pkns) cultured overnight to study the process of macrophage (monopoiesis) and neutrophil (granulopoiesis) development and the key gro ... | 2016 | 26546240 |
| effects of body chemical components on the allometric scaling of the resting metabolic rate in four species of cyprinids. | metabolic scaling is hypothesized to be affected by ontogenetic changes in the body chemical composition because several body components do not consume significant amounts of oxygen but contribute more to body mass (m) as a fish grows. we tested this hypothesis by assessing the mass scaling of the resting metabolic rate (rmr) and body compositions of lipid, protein, ash, and water in four species of cyprinids, hypophthalmichthys molitrix (m range 9.5-218.3 g), cyprinus carpio (m range 14.6-226.9 ... | 2016 | 26407956 |
| response of vitamins a, e, hematological and serum biochemical markers in crucian carp (carassius auratus gibelio) exposed to environmental pb(2+) and cd(2+). | toxicity of pb(2+) and cd(2+) is a widespread issue in the world; however, few studies have been conducted to understand their effect at environmentally realistic concentration in a mixture. in the present study, crucian carp was exposed to pb(2+) (30 µg·l(-1)), cd(2+) (100 µg·l(-1)) and their mixture (30+100 µg·l(-1)) for 96 h and 21 d period to assess changes in the liver and muscle vitamin a and e content, and hematological and serum biochemical parameters. the results indicated significant d ... | 2015 | 26336655 |
| induced all-female autotriploidy in the allotetraploids of carassius auratus red var. (♀) × megalobrama amblycephala (♂). | following activation by uv-irradiated bsb sperm, the fertilized eggs of tetraploid hybrids (abbreviated as 4nf1) (4n = 148, aabb) of carassius auratus red var. (abbreviated as rcc) (2n = 100, aa) (♀) × megalobrama amblycephala (abbreviated as bsb) (2n = 48, bb) (♂) developed into normal live gynogenetic offspring without chromosome doubling treatment. some of these were autotriploids with three sets of red crucian carp chromosomes (abbreviated as g1) (3n = 150, aaa). g1 were all-females, and can ... | 2015 | 26242753 |
| effects of dietary supplementation with astaxanthin and β-carotene on the semen quality of goldfish (carassius auratus). | this study was conducted to investigate the effects of two carotenoids (astaxanthin and β-carotene) on the sperm quality of goldfish carassius auratus (linnaeus, 1758). for this purpose, six diets containing concentrations of 50, 100, and 150 mg/kg of synthetic astaxanthin and β-carotene were added to a basic carp diet. one group of fish was also fed with a control diet (no added carotenoids). osmolality, spermatocrit value, and sperm concentration significantly increased in the treatment supple ... | 2015 | 26166170 |
| biological fate and effects of propranolol in an experimental aquatic food chain. | the aim of this study was to evaluate the trophic transfer of the β-blocker propranolol (prp) in an experimental aquatic food chain involving the green algae scenedesmus obliquus, the water flea daphnia magna and the crucian carp carassius auratus, as well as the metabolism and effects of prp in the liver of crucian carp. after a 48 h prp aqueous exposure for algae, with a subsequent 48 h dietary exposure for daphnia and an 8d dietary exposure for crucian carp, prp was observed in each trophic l ... | 2015 | 26057722 |
| expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (usp18) genes. | in mammals, usp18 (ubiquitin-specific protease 18) is an interferon (ifn) inducible protein and plays a role in regulation of ifn response upon viral infection. in this study, we first cloned a usp18 homologous gene from virally-infected crucian carp (carassius auratus) blastula embryonic (cab) cells, and later found in other fish species including zebrafish. all fish usp18 genes have 10 exons and 9 introns comparable to 11 exons and 10 introns in non-fish vertebrates. expression analysis reveal ... | 2015 | 25981749 |