| [the rubino reaction. criterion for inactivation in lepromatous patients]. | approximately 55% of active lepromatous patients respond positively to the rubino reaction. with arrested cases this rate of positivity lowers considerably to about 15%. in an effort to associate this reaction with the presence of bacilli, a study of 796 cases was undertaken. the patients were divided into: a control group, active tuberculoid cases, arrested tuberculoid cases, active borderline cases, arrested borderline cases, active lepromatous cases, and arrested lepromatous cases. the patien ... | 1983 | 6366399 |
| study of the use of nude mice in the cultivation of mycobacterium leprae in a normal, nonspecific pathogenic-free room at a temperature of 30-35 degrees c, without air-conditioning. | | 1983 | 6366412 |
| quantitative estimation of mycobacterium leprae in exhaled nasal breath. | | 1983 | 6366413 |
| lymphocyte suppression in leprosy induced by unique m. leprae glycolipid. | leprosy remains a significant medical and social problem in many developing countries. the varied forms of the disease form a spectrum. at one pole, tuberculoid leprosy, patients develop high levels of cell-mediated immunity which results in the killing and clearing of bacilli in the tissues. at the lepromatous pole, patients exhibit a selective immunological unresponsiveness to antigens of mycobacterium leprae so that the organisms inexorably multiply in the skin. we have suggested that in lepr ... | 1984 | 6366573 |
| parasitism of antigen presenting cells in hyperbacillary leprosy. | full thickness skin biopsies from four patients with borderline lepromatous leprosy (bl leprosy) have been examined. immunohistological techniques have been employed to analyse the non-lymphoid mononuclear cells present in the dermal infiltrates associated with the bl lesions. this analysis was performed using three monoclonal antibodies, rfd2 (recognizing macrophages), rfd1 (recognizing interdigitating cells) and na1/34 (recognizing langerhans cells). it was found that the vast majority of non- ... | 1984 | 6368061 |
| reversal reaction in lepromatous patients induced by a vaccine containing killed icrc bacilli--a report of five cases. | clinico-pathological features of five cases of lepromatous leprosy exhibiting "reversal" reaction with upgrading of lesions following vaccination with icrc vaccine have been described. two patients also developed enl. associated with the "reversal" reaction, the patients exhibited lepromin conversion. no evidence of fresh nerve lesions was observed in any patient. besides lepromin conversion, occurrence of "reversal" reaction is yet another evidence of immunogenicity of the icrc vaccine. | 1983 | 6368413 |
| rationales for vaccines against leprosy. | | 1983 | 6368414 |
| progress in the preparation of an antileprosy vaccine from armadillo-derived mycobacterium leprae. | | 1983 | 6368415 |
| animal vaccination studies with mycobacterium leprae. | | 1983 | 6368416 |
| antileprosy potentials of icrc vaccine. a study in patients and healthy volunteers. | | 1983 | 6368417 |
| two candidate antileprosy vaccines--current status of their development. | | 1983 | 6368418 |
| natural history of leprosy--aspects relevant to a leprosy vaccine. | | 1983 | 6368419 |
| epidemiological considerations in vaccine trials in leprosy. | | 1983 | 6368420 |
| operational problems in vaccine trials. | | 1983 | 6368421 |
| collection method for mycobacterium leprae from infected armadillo liver. | leprosy bacilli were separated from infected armadillo liver almost free from tissue contaminants by a percoll gradient centrifugation. the yield of bacilli was 46.7%. this is a very simple and effective method without enzyme treatment. | 1984 | 6368427 |
| variation of superoxide dismutase levels in extracts of mycobacterium leprae from armadillo liver. | recent improvements in the sensitivity of assay methods for superoxide dismutase (sod) have enabled the detection of this enzyme in 18 cell-free extracts of purified mycobacterium leprae. by converting back to units of sod obtained in the cytochrome c-based method previously used in work on this enzyme in mycobacteria, it was shown that extracts of m. leprae had 0.15-3.84 u sod/mg protein (this study). a mean value of 1.31 u/mg protein was calculated. it was not possible to find any factors whic ... | 1984 | 6368428 |
| 2-acetylpyridine thiosemicarbazones and mycobacterium leprae. | four 2-acetylpyridine thiosemicarbazones were tested in mice against mycobacterium leprae by the kinetic method and found to be nearly inactive in a dosage of 0.05% in the diet. at the same dosage, thiacetazone, as a positive control, exhibited its expected activity. | 1984 | 6368429 |
| immunity to leprosy and the mitsuda reaction. | | 1984 | 6368430 |
| trials of culturing of mycobacterium leprae using media enriched with thyroid hormones. | | 1983 | 6369009 |
| application of monoclonal antibodies towards immunological studies in leprosy. | | 1984 | 6369053 |
| effects of mycobacterium leprae antigens on the in vitro responsiveness of mononuclear cells from armadillos to concanavalin-a. | | 1984 | 6369054 |
| skin test activity of an antigen fraction prepared from mycobacterium leprae compared with standard lepromin and tuberculin ppd in leprosy patients. | | 1984 | 6369055 |
| an alternative route for infecting armadillos with mycobacterium leprae. | a nine-banded armadillo was inoculated with mycobacterium leprae in both hind footpads. the animals were usually inoculated intravenously, or intradermally in the abdominal skin. profuse multiplication of the bacilli occurred at the injection sites after more than two years. eventually bacteraemia developed, and large numbers of the organisms were found in skin biopsies and in lymph nodes. there was limited dissemination of the bacteria into the spleen and the liver, and peripheral nerve invasio ... | 1984 | 6369076 |
| suppression of the antibody response to sulfolipids in leprosy patients by 4,4'-diaminodiphenyl sulfone (dds). | antibodies to sulfolipids were demonstrated in patients suffering from lepromatous leprosy. the antibody titre was found to decrease gradually on treatment with dds. this effect was maximum for patients undergoing treatment for more than 1 year. | 1983 | 6370132 |
| immunization against leprosy: progress and prospects. | the limitations of the current approach to leprosy control through mass treatment of patients are well recognized. the long incubation period of the disease, the insidious onset, the chronic course, and the need for prolonged treatment have made control a formidable task. the recent years have seen tremendous progress in the field of immunology of leprosy, and the availability of large quantities of mycobacterium leprae, grown in the nine-banded armadillo, has given impetus to the search for a v ... | 1984 | 6370487 |
| [immunosuppression mechanisms in leprosy]. | | 1983 | 6371439 |
| taxonomy of mycobacterial strains isolated from the tissues of leprosy patients. | thirty-six slowly growing mycobacteria isolated from the tissues of leprosy patients were studied using 40 characteristics as well as susceptibility to 27 distinct mycobacteriophages. the composition in mycolic acids of selected strains was also studied. according to the data, the strains formed 5 clusters. some of the clusters were possibly as yet undescribed species; however, comparison of the data with the known properties of mycobacterium leprae leads to the conclusion that none of the strai ... | 1983 | 6372573 |
| antibodies to mycobacterial arabinomannan in leprosy: correlation with reactional states and variation during treatment. | an enzyme-linked immunosorbent assay was used to measure antibody to mycobacterial arabinomannan in serial serum specimens obtained over the initial 12-31 months of therapy from nine patients with leprosy. the antibody level in pretreatment sera was directly proportional to the quantity of mycobacterium leprae present in each patient as assessed by six-site scrapings (r = 0.75). the three patients with the lowest antibody levels (od 0.1-0.3) had uncomplicated courses and their levels declined sl ... | 1984 | 6373624 |
| adenosine triphosphate content of mycobacterium leprae from leprosy patients. | mycobacterium leprae obtained from randomly selected lepromatous leprosy patients were used to evaluate the atp assay technique for detecting viability of these cells. the findings were further confirmed by the standard mouse foot pad technique. while the latter takes about 8-12 months to obtain any valid information on the status of m. leprae, the atp data can be generated within hours and at much lower cost. it is hoped that the atp data could also instantaneously identify viable bacilli from ... | 1984 | 6373626 |
| attempts at cultivation of mycobacterium leprae in macrophages from susceptible animal hosts. | macrophages from nude mice, nude rats, and armadillos were cultured in vitro and examined for their ability to support mycobacterium leprae. no significant growth of this organism were observed after over 200 days of culture. no significant benefit was derived from modifying culture conditions or from variations in the source of macrophages or the source of m. leprae. | 1984 | 6373627 |
| ultrastructural features of macrophages of armadillos infected with actively multiplying mycobacterium leprae. | experimental leprosy lesions in the armadillo (dasypus novemcinctus) were studied by freeze etching and ultrathin sectioning. infected macrophages have distinct intracytoplasmic foamy structures in the form of spherical droplets accumulated around multiplying bacilli. this finding is the same as those observed in human lepra cells and nude mice macrophages infected with m. leprae. | 1984 | 6373628 |
| electron microscopic study of leprosy in a mangabey monkey (natural infection). | ultrastructural features of the leproma of a) a naturally infected mangabey monkey, and lepromas and liver of b) a passage mangabey monkey, c) a rhesus monkey, d) an african green monkey, and e) a nine-banded armadillo inoculated with leprosy bacilli isolated from the leproma of a naturally infected mangabey monkey were studied by the freeze-etching technique. the size, shape, and ultrastructural features of leprosy bacilli in the phagolysosomes of macrophages in all of these samples were essent ... | 1984 | 6373629 |
| multiple axonal myelination in the experimental mouse leprosy model. | | 1984 | 6373630 |
| oxidation of carbon sources through the tricarboxylic acid cycle in mycobacterium leprae grown in armadillo liver. | all the enzymes of the tricarboxylic acid cycle have now been demonstrated in extracts of mycobacterium leprae grown in armadillo liver. many were also present in homogenates of host-tissue, but biochemical evidence is presented which indicates that all enzymes detected in extracts from m. leprae were authentic bacterial enzymes. further evidence for a complete tricarboxylic acid cycle in m. leprae was obtained by first establishing that citrate could be taken up and catabolized by whole m. lepr ... | 1984 | 6374022 |
| in vitro studies on the effect of levamisole in lepromatous leprosy. | our previous studies have demonstrated a defective macrophage response to m. leprae in lepromatous leprosy patients. in the present study we report the restoration of fc receptor and hla-dr antigen expression as well as antigen specific macrophage-lymphocyte interaction on treatment with levamisole in vitro. these results indicate that levamisole activates the macrophages which in turn results in an improved cell mediated immune response in lepromatous leprosy. this may also be applicable in oth ... | 1984 | 6374154 |
| [new trends in the treatment of leprosy]. | | 1983 | 6374636 |
| [effect of ultrasonic waves on the growth intensity of mycobacterium leprae in liquid nutrient media]. | ultrasound-treated m. leprae grow in liquid culture media at a greater rate than untreated ones. ultrasound-treated m. leprae retain their capacity for intensive growth after their passage in mice. | 1984 | 6375218 |
| humans respond predominantly with igm immunoglobulin to the species-specific glycolipid of mycobacterium leprae. | the immunoglobulin classes of the antibody response to the species-specific phenolic glycolipid antigen of mycobacterium leprae have been characterized for serum specimens from 78 patients with leprosy. these patients included the entire clinical spectrum from paucibacillary to multibacillary disease, including polar tuberculoid (tt; 11 patients), borderline tuberculoid (bt; 15), borderline (bb; 17), borderline lepromatous (bl; 13), and lepromatous (ll; 22)--clinical classifications according to ... | 1984 | 6376654 |
| antigen specific macrophage-lymphocyte interaction in lepromatous leprosy. | peripheral blood derived macrophages from lepromatous leprosy patients were unable to interact with lymphocytes in the presence of m. leprae. this lack of interaction is probably not associated with membrane hla-dr antigens since trypsin and colchicine restored m. leprae induced depression in the latter but were unable to bring about a positive interaction. two possible defects exist therefore in the lepromatous macrophage. these are an innate inability to process and present m. leprae antigens ... | 1984 | 6376810 |
| [advances in the chemotherapy of leprosy]. | the chemotherapy of multibacillary leprosy with dapsone alone has resulted after 15-20 years in the selection and the diffusion of dapsone resistant mycobacterium leprae. to overcome dapsone resistance, to prevent the selection of organisms resistant to other drugs and to kill the largest proportion of sensitive m. leprae, the chemotherapy of leprosy must rely, as chemotherapy of tuberculosis, upon the combination of several drugs. rifampicin is included in all drug combinations recommended by w ... | 1984 | 6377002 |
| anti-mycobacterium leprae antibodies in urine from lepromatous patients examined by crossed immunoelectrophoresis and radioimmunoassay. | precipitating anti-mycobacterium leprae antibodies were found in concentrated urine samples from 21 out of 42 lepromatous patients. these antibodies were directed against m. leprae antigens 5, 6, and 7. in a radioimmunoassay for anti-m. leprae antibodies, 90% of these patients had higher antibody levels in their urine than control persons. there was a positive correlation between anti-m. leprae antibody levels in serum and urine. the advantages of using atraumatically collected samples like urin ... | 1984 | 6377478 |
| [structure of the lipid constituents of mycobacterium leprae. comparison with various species of mycobacteria]. | | 1983 | 6377796 |
| [action of desoxyfructo-serotonin on mycobacterium leprae. results of inoculation of human biopsy specimens in the mouse after a year of treatment]. | m. leprae, extracted from cutaneous biopsies of 4 out of 7 patients treated with dfs during one year at doses of 1000 to 1500 mg/day were injected in mouse foot pads. bacterial multiplication was observed in each case. the purely bacteriostatic activity of dfs for m. leprae, previously observed in mice, is thus confirmed. the eventual place of dfs in the therapy of leprosy is discussed. | 1983 | 6377799 |
| immunochemical characterization of mycobacterium leprae antigens by the sds-polyacrylamide gel electrophoresis immunoperoxidase technique (sgip) using patients' sera. | in this study the sds-polyacrylamide gel electrophoresis immunoperoxidase (sgip) assay was used for characterizing the antigenic components of mycobacterium leprae using patients' sera. this technique involved the separation of mycobacterial sonicates on sds-polyacrylamide gels, longitudinal sectioning of the gels, incubation with patients' sera and visualization of the antigen-antibody complexes by the indirect immunoperoxidase technique. a number of antigens present in m. leprae sonicates were ... | 1984 | 6378452 |
| c-reactive protein and apob containing lipoproteins are associated with mycobacterium leprae in lesions of human leprosy. | skin biopsies from patients with leprosy across the spectrum from tuberculoid (tt) to lepromatous (ll), including histoid lepromas and erythema nodosum leprosum (enl) reactions, were stained immunohistochemically for the presence of c-reactive protein (crp) and the apolipoprotein, apob. mycobacterium leprae bacillary material comprising cell walls, cytoplasmic and soluble components was present with increasing abundance towards the lepromatous end of the spectrum and always stained positively wi ... | 1984 | 6378453 |
| the effect of cortisone on mycobacterium leprae-infected mice. | | 1984 | 6379352 |
| dapsone-resistant leprosy. | | 1984 | 6379353 |
| [indirect immunofluorescence for detecting antibodies against m. leprae. application to a group of patients and persons living with them]. | | 1983 | 6379769 |
| [lipid composition of cultivable mycobacteria isolated from livers of armadillos infected by mycobacterium leprae]. | four bacterial strains isolated from two livers of armadillos experimentally infected with mycobacterium leprae were studied. lipids obtained after saponification and methylation and complex lipids obtained by solvent extraction were examined. the presence of mycolates showed that the four strains belonged to the genus mycobacterium, but the mycolate patterns, identical for the four strains, were different from those of all strains studied so far. three of these strains contained phthioceranic a ... | 1984 | 6380370 |
| fluorescence of mycobacterium leprae. | | 1984 | 6380513 |
| in vitro stimulation of lymphocytes with an antigen fraction prepared from mycobacterium leprae and tuberculin ppd in contacts and non-contacts of leprosy patients. | an antigen fraction from mycobacterium leprae, called mlw1, was used as stimulator in the lymphocyte stimulation test, for comparison with tuberculin ppd. the test was performed in three groups of contacts of leprosy patients with various degree of exposure: (1) close contacts, (2) healthy occupational contacts and (3) non-close contacts and, in addition, in a group of bcg vaccinated and non-exposed controls. the mlw1 preparation induced moderate to strong responses in all three groups of contac ... | 1984 | 6380833 |
| isolation and analysis of circulating immune complexes in leprosy. | circulating immune complexes (cic) were isolated by two antigen nonspecific methods from 60 leprosy patients belonging to borderline tuberculoid (bt) and lepromatous (ll) types with and without reactions. cic were elevated in both bt and ll reactions. cic from bt in reaction (btr) were found to consist largely of igg and c3, whereas, c-reactive protein could be found in cic from ll reactions (lr). in addition, igm and rheumatoid factor were demonstrated in the complexes of lr patients who had ma ... | 1984 | 6380842 |
| [the mitsuda reaction and its antigen, good subjects for a bibliography]. | some references of the history of mitsuda reaction and its antigen, as well as for the results and significance of this unique reaction are presented. in spite of the great advances of modern immunology. mitsuda reaction is still the best method for the diagnosis of the forms of hanseniasis and for the prognosis of the disease in patients and in healthy persons. | 1983 | 6381300 |
| [in vitro behavior of macrophages of healthy subjects and hansen patients against mycobacterium leprae]. | the in vitro phagocytic capacity of human macrophages facing m. leprae was analysed in a selected sample of healthy and hanseniasis patients (virchowian and tuberculoid) individuals. families where at least one of the spouses presented hansen's disease were selected and 176 white people were studied. the analysis of the macrophages' behavior was performed by reading the slides in the 5th, 10th, 15th, 20th and 30th days after inoculation of m. leprae. the classification is discussed. the results ... | 1983 | 6381301 |
| [familial analysis of the in vitro behavior of human macrophages against mycobacterium leprae]. | the lysogenic capacity of human macrophages facing m. leprae in vitro may be dependent on an important genetic component. although the family aggregation of the trait is demonstrated, this is a necessary but not sufficient condition to prove genetic influence. the data do not fit some simple genetic models (autosomal dominant or incompletely dominant gene; dominant or recessive sex-linked gene). the results obtained are consistent with the hypothesis that the macrophages' lysogenic capacity is m ... | 1983 | 6381302 |
| experimental mycobacterial infections of cba/n mice. | in an effort to assess the contribution of b-lymphocyte-mediated mechanisms to the immune responses to several mycobacteria, responses to these intracellular pathogens were compared in immunologically normal cba/cahn mice and in histocompatible cba/n (xid) mice, which exhibit abnormalities of b-lymphocyte function. swelling in response to local inoculation with mycobacterium marinum was significantly greater in the hind feet of cba/cahn mice than in those of cba/n mice, but the difference was ve ... | 1984 | 6381393 |
| ecological considerations on treatment of leprosy. | | 1983 | 6381451 |
| [growth inhibition of mycobacterium lepraemurium in a cell-free medium by adding a suspension of m. leprae]. | | 1983 | 6381452 |
| [susceptibility of rhino mouse to mycobacterium leprae]. | | 1983 | 6381457 |
| electrophysiological studies of the sciatic nerves in mycobacterium leprae foot pad-injected rats. | this study tested the possibility of developing an experimental model of neuropathy in female wistar rats inoculated with mycobacterium leprae in the foot pad and assessed by repeated electrophysiological methods. m. leprae multiplied in the rats but considerably less than in simultaneously inoculated mice. no acid-fast bacilli were found in nerves. motor and sensory conduction velocities remained normal at the thigh level of the sciatic nerve. at the leg, they decreased significantly bilaterall ... | 1984 | 6384077 |
| lactate dehydrogenase in mycobacterium leprae grown in armadillo liver. | lactate dehydrogenase (ldh) was detected in extracts of untreated and naoh-treated mycobacterium leprae. since armadillo liver ldh isoenzymes with a similar electrophoretic mobility were shown to be considerably more sensitive to inhibition by oxamate than ldh in m. leprae extracts, it was confirmed that m. leprae grown in armadillo liver has its own ldh. neither the activity of ldh in m. leprae nor its electrophoretic mobility supported the tentative suggestion that an "anomolous" ldh isoenzyme ... | 1984 | 6384078 |
| unresponsiveness to mycobacterium leprae in lepromatous leprosy in vitro: reversible or not? | | 1984 | 6384079 |
| modification of the fluorescent staining method for mycobacterial cells. | | 1984 | 6384149 |
| the mouse footpad test--sensitive to small proportions of drug-resistant bacilli in a sample of m. leprae. | in experiments at the radda barnen research laboratories of the slr & tc karigiri, the mouse footpad test was demonstrated to detect dds-resistant m.leprae even if as few as 0.1% (1 in 1000) of the m. leprae tested were dds-resistant. the mouse footpad test appears to be sensitive to minute proportions of drug-resistant bacilli in samples of m. leprae tested. | 1984 | 6384379 |
| human vaccination studies in normal and contacts of leprosy patients. | | 1984 | 6384380 |
| mechanism of immunosuppression in leprosy--macrophage membrane alterations. | l-lysate induced macrophage membrane alteration was studied using 3 membrane markers: (i) fc receptor, (ii) concanavalin a (con a) receptor, and (iii) m. leprae adherence to macrophage membrane. the data indicate that l-lysate induces membrane perturbation of normal macrophages. the alteration can be reversed with trypsin and colchicine. membrane alteration observed may lead to defective macrophage participation in a cell-mediated immune reaction. | 1984 | 6384522 |
| cell-mediated immunity in mice treated with mycobacterium leprae or with macrophages harbouring m. leprae. | following treatment of balb/c or c3h/hen mice in the hind footpads with irradiated mycobacterium leprae, a marked enhancement of natural killer (nk) activity was observed in cells from the draining popliteal lymph node or from the spleen. nk activity was further enhanced when the treatment consisted of killed m. leprae which had been incorporated into mouse peritoneal macrophages. this effect was noted as early as 2 weeks after treatment and persisted for at least 9 weeks. lymphoblastic transfor ... | 1984 | 6385816 |
| reversal by interleukin-2 of the t cell unresponsiveness of lepromatous leprosy to mycobacterium leprae. | in some subjects mycobacterium leprae causes disseminated (lepromatous) disease. such subjects show both in vivo and in vitro deficient t cell responses to m. leprae, but not to other antigens. we have recently shown that lepromatous peripheral blood mononuclear cells (pbmc) failed to produce interleukin 2 (il-2) in response to m. leprae and that t cell-conditioned media (tcm) can reverse the t cell unresponsiveness in a majority of lepromatous leprosy patients (haregewoin et al. 1983). here we ... | 1984 | 6386665 |
| comparison of biochemical characterisation of icrc bacilli with m. leprae: effect of substrate alteration in the medium. | icrc-bacilli strain c-44 when grown in dubos medium of its equivalent, express m. avium taxonomic biochemical characters. assuming that difference in characters of m. leprae and icrc bacilli, could be due to 'in vivo' and 'in vitro' milieu, we altered the substrates in the medium. the bacilli grow well in the new medium containing selenium, ferric nitrate, magnesium chloride and deleting tween 80. the icrc strain c-44 grown in new medium expressed characters: 9/10 similarity with m. leprae. the ... | 1984 | 6387003 |
| antigeneic cross-reactivity between icrc-bacilli and m. leprae--"in vitro" evaluation. | leucocyte migration inhibition, in presence of specific particulate antigen, is a good correlate of cmi. it can detect small differences in related antigens. in the present study, lmi was used to study the cross-reactivity between icrc bacilli and m. leprae and also to examine the antigenic relationship amongst different icrc isolates. the study showed a close antigenic cross-reactivity in lepromin and icrc-in. lmi has brought out strain differences in two icrc culture isolates, c-44 and c-75. t ... | 1984 | 6387004 |
| hypothesis: possible idiotypic suppression of cell-mediated immunity in lepromatous leprosy. | | 1984 | 6387354 |
| the discovery of mycobacterium leprae. a medical achievement in the light of evolving scientific methods. | the discovery of m. leprae by g. h. armauer hansen (1841-1912) in 1873 represents a link in a chain of development in international medicine that was influenced by two main concepts, namely, that germs may be causes of disease and that social conditions may be related to disease, either as causes or consequences or both. hansen's work is also a link in a chain of research on leprosy in norway. hansen met with serious challenges in addition to those that were purely scientific. to prove the causa ... | 1984 | 6388392 |
| three histologic fixatives for the demonstration of mycobacterium leprae. | | 1984 | 6388395 |
| mycobacterium leprae within a squamous cell carcinoma. | | 1984 | 6388398 |
| lytic potency against various mycobacterial strains of the phage isolated from mycobacterium leprae murium "douglas". | the lytic potency of a newly isolated phage al-1 obtained from the laboratory strain m. leprae murium "douglas" was examined. the phage was multiplied on the laboratory strain m. smegmatis atcc 607 and for the lytic test 0.1 ml of suspension containing pfu 10(5) was used. in the whole 18 mycobacterial strains both slowly and fast growing multiplied in liquid sula's medium were tested. for phage lytic tests two simple agar media and standard redmond's medium rva-24 were used. the examined slowly ... | 1984 | 6389043 |
| isolation of mycobacterial phage from the laboratory strain mycobacterium leprae murium "douglas". | the colony microstructure of the laboratory strain mycobacterium leprae murium "douglas" cultivated on ogawa's egg medium was examined. a bioptical sample from the liver of a white mouse subcutaneously infected and observed for ten months was used as inoculum. the inoculum contained 5.2 x 10(9) acidfast rods. the ogawa's media were incubated in 5% atmosphere of co2 at 33 degrees c to 37 degrees c for 6 to 10 months. the outgrown colonies were killed with a formol solution, then embedded into the ... | 1984 | 6389044 |
| phenolic glycolipid 1 of mycobacterium leprae causes nonspecific inflammation but has no effect on cell-mediated responses in mice. | the involvement of the phenolic glycolipid from mycobacterium leprae in cell-mediated immunity has been investigated in this study. the phenolic glycolipid itself does not appear to stimulate cell-mediated immunity directly, as shown by its failure to elicit a classical delayed-type hypersensitivity response in mice immunized with m. leprae or to stimulate m. leprae-immune lymph node cells in a lymphoproliferative assay. intradermal vaccination with the phenolic glycolipid failed to influence th ... | 1984 | 6389362 |
| the placenta in leprosy. | eighty-one placentae from women with leprosy and 17 placentae from healthy controls were subjected to a detailed macroscopic, light microscopic, ultrastructural, immunopathological, microbiological and biochemical study. the placental morphology and immunohistology were normal, and there was no morphological evidence of infection of the placenta due to m. leprae. no acid-fast bacilli or acid-fast bacillary granules were seen on light microscopy of any of the placentae from leprous women, althoug ... | 1984 | 6390420 |
| [possible mechanisms of cellular degeneration in macrophages infected with bcg in vitro and in leprous granulomas]. | | 1984 | 6390584 |
| evidence for the presence of m. leprae reactive t lymphocytes in patients with lepromatous leprosy. | evidence for the presence of mycobacterium leprae reactive t cells in many lepromatous leprosy (ll) patients was obtained using in vitro antigen-induced lymphoproliferative responses. (1) co-cultures of t enriched cells from ll patients when combined with 2 h adherent cells (ac) from hla-d compatible tuberculoid leprosy individuals showed significant levels of 3h-thymidine incorporation in the presence of soluble and integral m. leprae antigens. (2) more interestingly, autologous t cell + ac co- ... | 1984 | 6391762 |
| a dual effect of tilorone on multiplication of mycobacterium leprae in mice. | tilorone, a synthetic inducer of interferon found earlier to inhibit multiplication of mycobacterium leprae in the foot pad of the mouse while it enhanced infections of mice by m. lepraemurium and m. marinum, has been shown to exert a dual effect on m. leprae infection of the mouse. when administered continuously, incorporated into the mouse diet in a concentration of 0.015 g per 100 g diet, the drug was usually immunosuppressive, permitting enhanced multiplication of the organisms. when adminis ... | 1984 | 6395678 |
| phosphatidylinositomannosides in lepromatous leprosy nodules. | | 1984 | 6396226 |
| dapsone resistance in patients attending central leprosy teaching and research institute, chengalpattu (south india). | the central leprosy teaching and research institute (c.l.t. & r.i.) chengalpattu, took up studies on dapsone resistance in m. leprae from 1974. from 1978, the study was further strengthened by a project under thelep (tdr) for eliciting information on the efficacy of certain drug regimens. the thelep studies were to be conducted only on the dapsone sensitive untreated cases and, therefore, directed towards the detection of primary resistance, while the non-thelep institutional studies were concen ... | 1984 | 6396340 |
| the sensitivity and specificity of fluorescent leprosy antibody absorption (fla-abs) test for detecting subclinical infection with mycobacterium leprae. | | 1984 | 6396473 |
| anti-mycobacterium leprae antibodies induced by lepromin injection as demonstrated by indirect immunofluorescence. | | 1984 | 6396474 |
| the bactericidal activity of various aminoglycoside antibiotics against mycobacterium leprae in mice. | | 1984 | 6396475 |
| prevalence of secondary dapsone-resistant leprosy in upper volta. | | 1984 | 6396476 |
| leprosy immunology--some aspects of the role of the immune system in the pathogenesis of disease. | | 1984 | 6396478 |
| untreated hansen's disease of the eye: a clinicopathological report. | a maltese immigrant presented with intermittent bilateral anterior uveitis for which no cause could be found. the inflammation did not respond to topical treatment and ultimately the left eye developed a hypopyon and was enucleated. histological examination revealed granulomatous inflammation and large numbers of mycobacterium leprae throughout the anterior segment. occasional foci of inflammation containing mycobacterium leprae were found in the vitreous extending to the retina at the posterior ... | 1984 | 6397175 |
| the early serodiagnosis of leprosy. i. the use of counterimmunoelectrophoresis and enzyme-linked immunosorbent assay. | | 1984 | 6397799 |
| leprosy--in pursuit of a vaccine. | clinical leprosy is characterized by varying manifestations between tuberculoid and lepromatous leprosy. in the former state the patient is able to elicit a cell-mediated immune response whereas in the latter, there is usually a humoral response. an understanding of this immunological balance is crucial in the search for a vaccine which will control the disease. the development of a possible anti-mycobacterium leprae vaccine has been advanced by the isolation of organisms from the infected tissu ... | 1984 | 6397930 |
| monoclonal antibodies against mycobacterium leprae and their applications in leprosy research. | | 1984 | 6398337 |
| trophic changes and extensive dissemination in normal mice infected with human mycobacterium leprae. | closely bred swiss albino normal mice (lecca strain) were inoculated in the footpad with m. leprae, at room temperature. the animals were harvested at 3, 6 and 9 months post inoculation, and bacillary counts were made. trophic changes were observed in the tail-tips, ears, footpads and forepaws 12-14 months post inoculation in a group which was allowed to survive. the histopathological changes and bacillary infiltration was found in many tissues/organs. the possibility of studying this normal str ... | 1984 | 6398338 |
| induction of delayed-type hypersensitivity by icrc anti-leprosy vaccine and the adoptive transfer of cell-mediated immunity in mice. | the immunogenic potency of anti-leprosy icrc vaccine preparations was determined by foot pad enlargement in mice. female balb/c mice were sensitized by i.d. route with irradiated, heat-killed or live antigens of 2 strains (c-44 and c-75) of icrc bacilli and tested against lepromin and icrc-antigens. icrc antigens sensitized mice against lepromin; similarly, bcg and m. leprae sensitized mice, which showed good cross reactivity with icrc antigens. a mixture of irradiated icrc bacilli and live bcg ... | 1984 | 6398339 |
| hydrolytic enzymes in macrophages from leprosy patients in presence of mycobacterium leprae. | presence of mycobacterium leprae in association with in vitro cultured macrophages, from bacillary negative long term treated lepromatous leprosy patients, induces reduced level of protein and lowering of hydrolytic enzymes like p-glucuronidase, lysozyme and lactic dehydrogenase. alkaline phosphatase, on the other hand is increased. in the macrophages from normal healthy individuals or tuberculoid leprosy patients, presence of m.leprae increases both protein and levels of all the above enzymes. ... | 1984 | 6398340 |
| extended studies on subclinical infection in leprosy. | | 1984 | 6398341 |
| central nervous system involvement in leprosy. | | 1984 | 6398342 |
| primary dapsone resistant leprosy in nepal. | mouse foot pad testing for primary dapsone resistant leprosy was performed in 15 patients. 13 were resistant (87%). two patients below 10 years were living with lepromatous leprosy mothers, one male aged 21, was born in kokana leprosarium and ten others gave no history of known contact of leprosy. | 1984 | 6398343 |
| prevalence of secondary dapsone resistance in nepal. | between 1980-1982, 56 patients suspected of developing dapsone resistant leprosy were seen at the skin clinic of anandaban leprosy hospital and kokana leprosarium. out of 56, 16 control mice had no growth. 29 patients (72.5%) were proved resistant by mouse foot pad tests. | 1984 | 6398344 |
| role of dapsone in chemotherapy of leprosy--a comparison of responses to therapy in two cohorts in 1960s and 1970s. | two cohorts of bacterio-positive cases registered and started on treatment during 1960-62 and 1968-70 were studied for differences in their response to dds monotherapy. proportion of male and female cases, mean bacteriological index (b.i.) and the distribution of cases according to the initial bi did not differ between the two cohorts. 49.7% of the patients in the ii cohort were taking regular treatment against 27.4% in the i cohort. at the end of 7 years of treatment, proportion of the cases th ... | 1984 | 6398345 |