| cloning and porin activity of the major outer membrane protein p1 from coxiella burnetii. | coxiella burnetii, the etiological agent of q fever, is a gram-negative obligate intracellular bacterium. two striking characteristics of this microorganism are its ability to thrive within a phagolysosome and its ability to persist in the environment outside a host cell. these abilities have been attributed to the existence of c. burnetii developmental cycle variants: large-cell variants (lcv), small-cell variants (scv), and small dense cells (sdc). variants differ in protein profiles, includin ... | 2002 | 12438349 |
| ifn-gamma-induced apoptosis and microbicidal activity in monocytes harboring the intracellular bacterium coxiella burnetii require membrane tnf and homotypic cell adherence. | ifn-gamma is critical for the protection against intracellular bacteria through activation of the antimicrobial machinery of phagocytes. coxiella burnetii, the etiological agent of q fever, is a strictly intracellular bacterium that inhabits monocytes/macrophages. we previously showed that ifn-gamma induced c. burnetii killing by promoting the apoptosis of infected monocytes. we show in this study that ifn-gamma-induced apoptosis of infected monocytes was characterized by a time- and dose-depend ... | 2002 | 12444137 |
| factsheet: q fever. | | 2002 | 12451405 |
| q fever: still a mysterious disease. | | 2002 | 12454328 |
| rickettsial, ehrlichial and bartonella infections of the myocardium and pericardium. | myocarditis and pericarditis are uncommon complications of human rickettsial, ehrlichial and bartonella infections. myocardial inflammation usually occurs in the setting of acute disseminated infection. organisms associated with myocarditis include: rickettsia rickettsii, r. conorii, orientia tsutsugamushi, coxiella burnetii, anaplasma phagocytophila (the causative agent of human granulocytic ehrlichiosis) and bartonella henselae. pericarditis has been described in the setting of r. conorii and ... | 2003 | 12456377 |
| mononeuritis multiplex caused by coxiella burnetii infection (q fever). | after 1 week of flu-like illness, a 64-year-old man developed rapidly progressive mononeuritis multiplex involving the right arm and both legs. serologic studies identified coxiella burnetii as the cause of the febrile disease (q fever). fourteen days doxycycline treatment (200 mg daily) induced rapid and complete recovery. after 6 months, flu-like symptoms, weakness and hypalgesia of the right leg reappeared. antibody titers again identified q fever. doxycycline was re-established and induced p ... | 2002 | 12460144 |
| q fever--a forgotten disease? | | 2002 | 12467686 |
| acute q fever and brachial neuritis: case report and literature review. | peripheral nervous system complications of q fever are uncommon. a case of electrophysiologically documented brachial neuritis occurring during acute coxiella burnetii infection is reported. the relevant literature is reviewed. | 2002 | 12478333 |
| current laboratory diagnosis of q fever. | q fever is a worldwide zoonosis caused by the strictly intracellular bacterium coxiella burnetii. among symptomatic patients (one-half of patients remain asymptomatic), acute q fever most frequently manifests as a self-limited febrile illness, pneumonia, or hepatitis. endocarditis is the predominant form of chronic q fever. all the classical techniques of bacteriology may be used for diagnosis of c burnetii infection. nonetheless, because of the risk of contamination, isolation must be performed ... | 2002 | 12491231 |
| use of macrolides for q fever. | | 2003 | 12499237 |
| atypical pathogens in adult patients admitted with community-acquired pneumonia in korea. | this study examined the prevalence of atypical pathogens causing community-acquired pneumonia (cap) in korea. we collected sera and clinical data for a period of 1 year for the adult patients consecutively admitted to chunchon sacred heart hospital with cap. the diagnosis was made using serologic methods to detect antibodies for mycoplasma pneumoniae, chlamydia pneumoniae, legionella spp., chlamydia psittaci, and coxiella burnetii. among 81 recruited patients, c. pneumoniae (n = 10, 12.3%) was t ... | 2002 | 12501255 |
| [prevalence and clinical characterization of coxiella burnetii infection in patients with protracted low-grade fever]. | we report here a persistent form of coxiella burnetii infection. there have been no prospective surveys of chronic c. burnetii infection reported in japan. until recently, it was not possible to distinguish between previous and current infection with serological tests for antibody to c. burnetii. the nested pcr method, however, allows us to appreciate the current infection by detecting c. burnetii dna with high sensitivity. inoculation method using an a/j mouse was performed to confirm the viabi ... | 2002 | 12508473 |
| pcr-based detection of coxiella burnetii from clinical samples. | | 2003 | 12512362 |
| update on q fever, including q fever endocarditis. | | 2002 | 12520650 |
| acute transverse myelitis associated with coxiella burnetii infection. | | 2002 | 12532936 |
| granulomatous lymphadenitis as a manifestation of q fever. | | 2003 | 12533300 |
| has coxiella burnetii (q fever) been introduced into new zealand? | | 2003 | 12533301 |
| [q fever in gran canaria: 40 new cases]. | the aim of this study was to describe the clinical and epidemiologic features of q fever in the southern area of the island of gran canaria (spain). | 2003 | 12550040 |
| molecular diagnosis of infective endocarditis by pcr amplification and direct sequencing of dna from valve tissue. | we used broad-range eubacterial pcr amplification followed by direct sequencing to identify microbial pathogens in heart valve material from 29 patients with histologically confirmed infective endocarditis and 23 patients free of infective endocarditis. microorganisms cultured by conventional techniques matched those identified by pcr in 21 cases. pcr alone identified the causative agent in three cases (streptococcus bovis, staphylococcus cohnii, and coxiella burnetii), allowing better patient m ... | 2003 | 12574279 |
| blood culture negative endocarditis: analysis of 63 cases presenting over 25 years. | to analyse cases of blood culture negative endocarditis (bcne) seen at st thomas' hospital, london, between 1975 and 2000. | 2003 | 12591823 |
| nitric oxide partially controls coxiella burnetii phase ii infection in mouse primary macrophages. | in most primary or continuous cell cultures infected with the q-fever agent coxiella burnetii, bacteria are typically sheltered in phagolysosome-like, large replicative vacuoles (lrvs). we recently reported that only a small proportion of mouse peritoneal macrophages (pmphi) infected with a nonvirulent, phase ii strain of c. burnetii developed lrvs and that their relative bacterial load increased only slowly. in the majority of infected pmphi, the bacteria were confined to the small vesicles. we ... | 2003 | 12595436 |
| variation in immune response genes and chronic q fever. concepts: preliminary test with post-q fever fatigue syndrome. | acute primary q fever is followed by various chronic sequelae. these include subacute q fever endocarditis, granulomatous reactions in various organs or a prolonged debilitating post-infection fatigue syndrome (qfs). the causative organism, coxiella burnetii, persists after an initial infection. the differing chronic outcomes may reflect variations within cytokine and accessory immune control genes which affect regulation of the level of persistence. as a preliminary test of the concept we have ... | 2003 | 12595908 |
| [three imported cases of acute q fever after an inspection tour to australia and new zealand]. | after an inspection tour to farms and abattoirs in australia and new zealand, three japanese persons simultaneously developed febrile illnesses in japan. they generally had slight fever and general fatigue, followed by thrombocytopenia and hepatic dysfunction. however, no respiratory symptoms were observed. in one of the cases, severe thrombocytopenia (1.3 x 10(4)/microliter) and high fever up to 40 degrees c were observed. these clinical symptoms were compatible with q fever. all of the cases s ... | 2002 | 12607350 |
| [rickettsiosis: state of the art at the turn of the 21st century]. | information about changes in the modern taxonomy of intracellular bacteria conditionally united within the nomination of "rickettsioses" is presented in the paper. due to a total hobby related with keeping home animals (pets), cats and dogs, apart from the cattle, joined the natural ecological cycles of rickettsioses stimulation. the morbidity of rickettsioses of the acaroid group has been persistently growing; like in case with other pathologies (lyme's disease) involving the acaroid transfer f ... | 2003 | 12608082 |
| q fever in adults: review of 66 clinical cases. | sixty-six cases of q fever in adults, serologically confirmed by indirect immunofluorescence, were studied to analyze the epidemiological, clinical and therapeutic aspects of the disease. eighty-three percent of the patients were male, and the mean age was 44.7 years. contact with animals was recorded in 24 patients. the main clinical form of presentation was pneumonia (37 cases); eight patients had hypoxia, and five had respiratory failure. the empirical treatment consisted of macrolides in 36% ... | 2003 | 12627285 |
| outbreak of q fever in lohra-rollshausen, germany, spring 1996. | q fever is an acute (and sometimes chronic) febrile illness caused by the rickettsial organism coxiella burnetii. the commonest animal reservoirs for c. burnetiiare cattle, sheep, and goats. infected animals shed the organisms, which resist desiccation, i | 1997 | 12631826 |
| urban outbreak of q fever, briancon, france, march to june 1996. | q fever is an ubiquitous zoonosis caused by the rickettsial organism coxiella burnetii. both sporadic cases and epidemics occur in areas where sheep and goats are bred. the main route of transmission is by inhalation of aerosols from the environment (soil | 1997 | 12631827 |
| acidification modulates the traffic of trypanosoma cruzi trypomastigotes in vero cells harbouring coxiella burnetii vacuoles. | we studied the fate of different trypanosoma cruzi trypomastigote forms after they invade vero cells persistently colonised with coxiella burnetii. when the invasion step was examined we found that persistent c. burnetii infection per se reduced only tissue-culture trypomastigote invasion, whereas raising vacuolar ph with bafilomycin a1 and related drugs, increased invasion of both metacyclic and tissue-culture trypomastigotes when compared with control vero cells. kinetic studies of trypomastig ... | 2003 | 12633656 |
| [bacillus anthracis, prions, coxiella burnetii]. | the detection kits for bacillus anthracis, an isoform of host prion and coxiella burnetii, using genetic technology are still not generalized. for b. anthracis target genes for detection would be genes of toxins. although it is difficult to detect prions related to prion diseases before death, some mutants of prion gene in leukocytes and 14-3-3 proteins in cerebrospinal fluid are detectable in living patients suffering from creutzfeldt-jakob disease. the gene of superoxide dismutase in coxiella ... | 2002 | 12652794 |
| urban zoonoses caused by bartonella, coxiella, ehrlichia, and rickettsia species. | the last half of the 20th century witnessed an increase in the occurrence and recognition of urban zoonoses caused by members of the genera bartonella, coxiella, ehrlichia, and rickettsia, all traditionally considered to be members of the family rickettsiaceae. in recent years, new human pathogens (bartonella elizabethae, bartonella henselae, and rickettsia felis) have been recognized in urban environments. other newly recognized pathogens (ehrlichia chaffeensis and ehrlichia phagocytophila in t ... | 2001 | 12653141 |
| cardiac valves in patients with q fever endocarditis: microbiological, molecular, and histologic studies. | the pathologic features of q fever endocarditis, which is caused by coxiella burnetii, were histologically evaluated in cardiac valves from 28 patients. we used quantitative image analysis to compare valvular fibrosis, calcifications, vegetations, inflammation, and vascularization due to q fever endocarditis with that due to non-q fever endocarditis and valvular degeneration. we also studied the presence of c. burnetii in valves by immunohistochemical analysis, culture, and polymerase chain reac ... | 2003 | 12660924 |
| dysregulation of cytokines in acute q fever: role of interleukin-10 and tumor necrosis factor in chronic evolution of q fever. | q fever manifests as primary infection or acute q fever and may become chronic in patients with underlying valvulopathy. because coxiella burnetii infection depends on host response, we measured tumor necrosis factor (tnf), interleukin (il)-6, il-12, and il-10 in patients with different clinical presentations of acute q fever. compared with control subjects, patients with uncomplicated acute q fever exhibited increased release of the 4 cytokines. their amounts were higher in patients with hepati ... | 2003 | 12660942 |
| [community-acquired pneumonia in patients in 2 hospital populations]. | patients hospitalized with community acquired pneumonia were studied prospectively in two hospitals located in the surroundings of buenos aires city. fifty two patients from general hospital manuel belgrano (hmb) were included from march 1998 to february 1999 and 23 patients from hospital dr a. cetrangolo (hcet) for respiratory disease, were included from june 2000 to may 2001. patients with lung tuberculosis, lung neoplasia and hiv infection were excluded. clinical background, signs and symptom ... | 2003 | 12673953 |
| q fever: a recent 'outbreak' in townsville. | | 2003 | 12680991 |
| [hepatitis as unique manifestation of q fever: clinical and epidemiologic characteristics in 109 patients]. | to describe the clinical and epidemiologic characteristics of hepatic involvement in a cohort of 109 patients with q fever. | 2003 | 12681131 |
| use of monoclonal antibodies to lipopolysaccharide for antigenic analysis of coxiella burnetii. | antigenic differences among coxiella burnetii strains were analyzed. the monoclonal antibodies against the lipopolysaccharide outer core did not react with the strains containing a qprs plasmid or with plasmidless strains, whereas they reacted with strains containing a qph1 or qpdv plasmid. c. burnetii isolates could be divided into two groups immunologically. | 2003 | 12682176 |
| coxiella burnetii avoids macrophage phagocytosis by interfering with spatial distribution of complement receptor 3. | phagocytosis is a highly localized event requiring the formation of spatially and temporally restricted signals. numerous microorganisms have taken advantage of this property to invade host cells. coxiella burnetii, the agent of q fever, is an obligate intracellular bacterium that has developed a survival strategy in macrophages based on subversion of receptor-mediated phagocytosis. the uptake of c. burnetii is mediated by alpha(v)beta(3) integrin and is restricted by impaired cross-talk of alph ... | 2003 | 12682255 |
| acute acalculous cholecystitis associated with q fever: report of seven cases and review of the literature. | q fever is a worldwide-occurring zoonosis caused by coxiella burnetii. there are various clinical manifestations of acute q fever, of which acute cholecystitis is a very rare clinical presentation. this study reports seven cases of acute cholecystitis associated with coxiella burnetii and reviews two other cases from the literature. all patients were admitted to hospital for fever and abdominal pain in the right upper quadrant. abdominal echography showed a distended gallbladder with biliary slu ... | 2003 | 12687414 |
| the fossil tabanids (diptera tabanidae): when they began to appreciate warm blood and when they began transmit diseases? | a discussion of the known fossil tabanids (diptera tabanidae) is presented based on fossil evidence. this includes the origin of the hemathophagy in the brachycera, more specifically for tabanids. several tabanid species in the extant fauna are vectors for disease-producing organisms that affect humans and animals. bacteria, viruses, rickettsiae, protozoa, and filarial worms can be transmitted by them, causing such diseases as anthrax, tularemia, anaplasmosis, various forms of trypanosomiasis, q ... | 2003 | 12687759 |
| infective endocarditis not related to intravenous drug abuse in hiv-1-infected patients: report of eight cases and review of the literature. | to add to the limited information on infective endocarditis (ie) not related to intravenous drug abuse (ivda) in hiv-1-infected patients. | 2003 | 12691542 |
| q fever--still a query and underestimated infectious disease. | coxiella burnetii (c.b.) is a strictly intracellular, gram-negative bacterium. it causes q fever in humans and animals worldwide. the animal q fever is sometimes designated "coxiellosis". this infection has many different reservoirs including arthropods, birds and mammals. domestic animals and pets, are the most frequent source of human infections. q fever may appear basically in two forms, acute and chronic (persistent). the latter form of q fever in animals is characteristic by shedding c.b. i ... | 2002 | 12693856 |
| complete genome sequence of the q-fever pathogen coxiella burnetii. | the 1,995,275-bp genome of coxiella burnetii, nine mile phase i rsa493, a highly virulent zoonotic pathogen and category b bioterrorism agent, was sequenced by the random shotgun method. this bacterium is an obligate intracellular acidophile that is highly adapted for life within the eukaryotic phagolysosome. genome analysis revealed many genes with potential roles in adhesion, invasion, intracellular trafficking, host-cell modulation, and detoxification. a previously uncharacterized 13-member f ... | 2003 | 12704232 |
| [time course of the levels of antibodies to coxiella burnetii and detection of c. burnetii-dna in three imported cases of acute q fever]. | three patients developed acute q fever after returning from an inspection tour of farms and abattoirs to australia. serum levels of antibodies to coxiella burnetii and the presence of c. burnetii-dna in blood samples were examined for more than 100 days. four-fold raises of igm and igg antibodies against c. burnetii phase 2 were observed within the first three weeks in all the three cases. maximum titers of igm and igg antibodies were 1,024-2,048 and 512-4,096, respectively. according to the tem ... | 2003 | 12708004 |
| detection of antibodies against spotted fever group rickettsia (sfgr), typhus group rickettsia (tgr), and coxiella burnetii in human febrile patients in the philippines. | a total of 157 sera from febrile patients in the philippine general hospital in manila, luzon, and the northern samar provincial hospital, the philippines, were used. serum antibodies against spotted fever group rickettsia (sfgr) and typhus group rickettsia (tgr) were detected by indirect immunofluorescence test. antibody positive rates were 1.3% for sfgr (rickettsia japonica) and 2.5% for tgr (r. typhus), respectively. rickettsial antibodies in humans in the philippines were found for the first ... | 2003 | 12711823 |
| [germs and toxins in bioterrorism]. | | 2003 | 12722194 |
| [q fever]. | | 2003 | 12722271 |
| [modern possibilities of bioterrorism diagnosis]. | one of the most important components of early detecting and response to biological attack are microbiological diagnostic methods. in order to provide the effectiveness of diagnosis continuous training in detection of potentially dangerous bioterrorist agents is needed. basic information about selected microbiological agents most dangerous for public health, belonging to a and b cdc categories are presented in the article. the most potent biological weapon mentioned in the last decade is anthrax. ... | 2003 | 12728676 |
| [renal failure as a complication of acute q fever]. | | 2003 | 12729532 |
| evaluation of coxiella burnetii antibiotic susceptibilities by real-time pcr assay. | coxiella burnetii is an obligate intracellular bacterium. the inability to cultivate this organism on axenic medium has made calculation of infectious units challenging and prevents the use of conventional antibiotic susceptibility assays. a rapid and reliable real-time pcr assay was developed to quantify c. burnetii cells from j774.16 mouse macrophage cells and was applied to antibiotic susceptibility testing of c. burnetii nine mile, phase i. for calculation of bacterial replication, real-time ... | 2003 | 12734219 |
| q fever in humans and animals in the united states. | coxiella burnetii, the etiologic agent of q fever, is a worldwide zoonotic pathogen. although q fever is present in the united states, little is known about its current incidence or geographic distribution in either humans or animals. published reports of national disease surveillance, individual cases, outbreak investigations, and serologic surveys were reviewed to better characterize q fever epidemiology in the united states. in national disease surveillance reports for 1948-1986, 1,396 human ... | 2002 | 12737547 |
| detection of coxiella burnetii by pcr in mice after administration of live m-44 vaccine. | original primers were prepared for the detection of coxiella burnetii by pcr for the amplification of a 752-bp fragment of dnaj gene. using these primers we observed the persistence of c. burnetii in different organs of mice after the administration of live q-fever vaccine over a 7-months period. | 2003 | 12744085 |
| identification of beta-subunit of bacterial rna-polymerase--a non-species-specific bacterial protein--as target of antibodies in primary biliary cirrhosis. | several observations suggest that bacteria induce autoimmunity in primary biliary cirrhosis (pbc). since no pbc-specific bacterial species could be identified, it can be speculated that the triggers are non-species-specific bacterial proteins. this hypothesis would imply that several or even all bacterial species can trigger pbc. therefore, we investigated whether pbc exhibits immune reactions to non-species-specific bacterial antigens. yersinia enterocolitica o3 was screened for the presence of ... | 2003 | 12757171 |
| coxiella burnetii pneumonia. | this report reviews the pulmonary and extrapulmonary manifestation of infections due to coxiella burnetii. q fever, a zoonosis, is due to infection with c. burnetii. this spore-forming microorganism is a small gram-negative coccobacillus that is an obligate intracellular parasite. the most common animal reservoirs are goats, cattle, sheep, cats, and occasionally dogs. the organism reaches high concentrations in the placenta of infected animals. aerosolisation occurs at the time of parturition an ... | 2003 | 12762362 |
| [another case of west nile fever in the netherlands: a man with encephalitis following a trip to canada]. | shortly after his return to the netherlands from a trip to ontario, a part of canada where infection with west-nile virus has been reported, a 69-year-old man became increasingly confused and generally unwell, accompanied by fever. the clinical picture was compatible with viral encephalitis and this was supported by eeg findings and the results of the cerebrospinal-fluid examination. mri of the brain did not contribute to the diagnosis. the patient was treated with aciclovir because herpes simpl ... | 2003 | 12784533 |
| syndromic surveillance using automated collection of computerized discharge diagnoses. | the syndromic surveillance information collection (ssic) system aims to facilitate early detection of bioterrorism attacks (with such agents as anthrax, brucellosis, plague, q fever, tularemia, smallpox, viral encephalitides, hemorrhagic fever, botulism toxins, staphylococcal enterotoxin b, etc.) and early detection of naturally occurring disease outbreaks, including large foodborne disease outbreaks, emerging infections, and pandemic influenza. this is accomplished using automated data collecti ... | 2003 | 12791784 |
| rhipicephalus ticks infected with rickettsia and coxiella in southern switzerland (canton ticino). | ticks of the rhipicephalus sanguineus species complex may be vector of various pathogens including rickettsia conorii (the etiological agent of the mediterranean spotted fever) and coxiella burnetii (cause of the query (q) fever). r. sanguineus ticks have been imported in several parts of central and northern europe, especially in environments such as kennels and houses providing the appropriate microclimatic conditions and the blood source necessary for their survival. since 1940 these ticks ha ... | 2002 | 12797987 |
| seroprevalence of infection by coxiella burnetii in canary islands (spain). | the aims of our study were: (i) to know the seroprevalence of coxiella burnetii infection in the canary islands, (ii) to evaluate its epidemiologic features and (iii) to compare the rates of seroprevalence using two different cut-offs (1:20 and 1:80) for the diagnosis of past infection. | 2003 | 12800952 |
| detection of coxiella burnetii in ticks collected in slovakia and hungary. | a total of 235 adult ticks collected from vegetation in slovakia and hungary in 1998-2000 were tested for coxiella burnetii by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). c. burnetii was identified in six ticks of ixodes ricinus, dermacentor marginatus, and haemaphysalis concinna species. | 2003 | 12800953 |
| natural history of q fever in goats. | during the spring of 1999, an outbreak of q fever resulted in 30 abortions among 174 (17%) goats in a caprine cooperative in newfoundland. the intent of this study was to determine the natural history of coxiella burnetii infection in goats. twenty-four goats on one farm were followed through the next two kidding seasons following the q fever outbreak. antibodies to phases i and ii c. burnetii were determined using an indirect immunofluorescence assay and samples of placentas were cultured for c ... | 2003 | 12804376 |
| maturation of the coxiella burnetii parasitophorous vacuole requires bacterial protein synthesis but not replication. | this study examined whether protein synthesis and replication are required for maturation and fusogenicity of the lysosomal-like, large and spacious parasitophorous vacuole (pv) of coxiella burnetii, an obligate intracellular bacterium. large and spacious pv with multiple non-replicating c. burnetii were observed by phase microscopy in vero cells infected at a multiplicity of infection of ten and treated with a bacteriostatic concentration of nalidixic acid or carbenicillin, antimicrobics that i ... | 2003 | 12814437 |
| functional similarities between the icm/dot pathogenesis systems of coxiella burnetii and legionella pneumophila. | coxiella burnetii, the etiological agent of q fever, is an obligate intracellular pathogen, whereas legionella pneumophila, the causative agent of legionnaires' disease, is a facultative intracellular pathogen. during infection of humans both of these pathogens multiply in alveolar macrophages inside a closed phagosome. l. pneumophila intracellular multiplication was shown to be dependent on the icm/dot system, which probably encodes a type iv-related translocation apparatus. recently, genes hom ... | 2003 | 12819052 |
| q-fever serologic diagnostics with inhabitants of canton of sarajevo 2001 year. | q-fever is widely spread antropoozosis caused by coxiellae burnetti, an intracellular compulsory microbe parasite. two characteristics of coxiellae burnetti are of crucial importance for appearance of q-fever, especially in the circumstances when the cause of infection remains unclear. the first one is a high resistance of coxiellae burnetti to environment changes and the second one is a small size of the infectious dose. the clinical manifestations of q-fever can vary, so the making of diagnosi ... | 2003 | 12822377 |
| the devil is in the detail. | | 2003 | 12823941 |
| epidemiological survey on the route of coxiella burnetii infection in an animal hospital. | the source of q fever infection, was investigated by serological and polymerase chain reaction (pcr) analysis of specimens from humans and pets in an animal hospital. two animal health technicians showed a positive serological reaction against coxiella burnetiiat the start. one of the two positive subjects remained pcr-positive for about 1 year and the other converted to pcr-negative, but the igg antibody titer remained at 1 : 128 after minocycline treatment. among animals housed in the hospital ... | 2003 | 12825114 |
| the effect of c. burnetii infection on the quality of life of patients following an outbreak of q fever. | sixty-six cases of q fever were diagnosed in people affiliated with a goat-farming co-operative in rural newfoundland in the spring of 1999. follow-up studies which included administration of the short form 36 health survey (sf-36) were conducted 3 and 27 months after the initial outbreak to prospectively follow the effects of acute q fever on the quality of life of the participants. twenty-seven months after the outbreak 51% of those who had q fever reported persistent symptoms including seven ... | 2003 | 12825734 |
| q fever in human and livestock populations in oman. | in 2000, q fever was documented for the first time in the sultanate of oman in two patients, one with chronic pericarditis and the other with acute pneumonia. in 2001, a study of a randomly selected group of 102 adult patients from different provinces in northern oman, presenting to the university hospital in muscat with unrelated conditions (e.g., diabetes mellitus, ischemic heart disease), revealed that 10 (9.8%) were seropositive for previous coxiella burnetii infection. examination of sera f ... | 2003 | 12860629 |
| molecular evidence of coxiella-like microorganism harbored by haemaphysalis concinnae ticks in the russian far east. | twenty female imago of haemaphysalis concinnae ticks collected from wild vegetation in may of 2001 in the obor region near khabarovsk in the russian far east were studied. dna was extracted with commercially available kits following manufacturer's instructions. we used broad range primers sfg3 and sfg6 proposed for amplification of 16s ribosomal rna gene's portion of spotted fever group rickettsia. initial amplification resulted in positive results in all 20 ticks. direct sequencing of five rand ... | 2003 | 12860630 |
| investigation of a focus of q fever in a nonfarming population in the federation of bosnia and herzegovina. | | 2003 | 12860631 |
| rapid deployment and operation of a q fever field laboratory in bosnia and herzegovina. | in may 2000, cdc was asked to assemble a team to investigate reports of a widespread outbreak of q fever (coxiella burnetii infection) in bosnia-herzegovina. brucellosis was also suspected in the outbreak. in a short period of time, we were able to gather supplies, establish collaboration, and rapidly deploy a field laboratory for the serodiagnostic testing of animal and human specimens. strategy, problems, and insights on organization, transport, and deployment will be discussed. | 2003 | 12860645 |
| significance of q fever serologic diagnosis in clinically suspect patients. | q fever is caused by c. burnetii, an intracellular obligate bacterium. for clinical confirmation of q fever, diagnosis of interstitial pneumonia is of significance. the acute disease varies in severity from minor to fatal, with the possibility of serious complications. chronic endocarditis is a well-known outcome. symptoms of q fever can vary; fixing diagnosis is done by serology with the phase i and the phase ii antibody. we tested 44 sera of 31 clinically suspect patients. from these, 22 patie ... | 2003 | 12860655 |
| interleukin-4 induces coxiella burnetii replication in human monocytes but not in macrophages. | coxiella burnetii, an obligate intracellular bacterium, is the agent of q fever. the chronic disease is characterized by impaired cell-mediated immune response and microbicidal activity of monocytes. we hypothesized that interleukin(il)-4, a th2 cytokine, interferes with the fate of c. burnetii inside monocytes. c. burnetii survived without multiplication in resting monocytes, but replicated in il-4-treated monocytes. the effect of il-4 is specific for monocytes since il-4 did not stimulate c. b ... | 2003 | 12860673 |
| differences in cytokine mrna profiles between naïve and in vivo-primed ovine pbmc after exposure to heat-inactivated coxiella burnetii. | during human coxiella burnetii (c. burnetii) infections, high il-10 levels favor replication of c. burnetii in monocytes and development of chronic q fever, whereas ifn-gamma promotes intracellular killing. sheep are a common source for human c. burnetii infections, but in contrast to man become transiently infected only. in a first approach to unravel the role of cytokines during ovine c. burnetii infections, we investigated by semiquantitative rt-pcr whether heat-inactivated c. burnetii affect ... | 2003 | 12860674 |
| coxiella burnetii persistence in three generations of mice after application of live attenuated human m-44 vaccine against q fever. | | 2003 | 12860680 |
| influence of inactivated coxiella burnetii on production of proinflammatory cytokines by blood cells from healthy donors. | | 2003 | 12860681 |
| studies on the immunological role of virenose and dihydrohydroxystreptose present in the coxiella burnetii phase i lipopolysaccharide. | | 2003 | 12860682 |
| identification and cloning potentially protective antigens of coxiella burnetii using sera from mice experimentally infected with nine mile phase i. | coxiella burnetii is an obligate intracellular bacterium that causes acute q fever and occasional chronic infections in humans. to determine the immunodominant antigens during infection with c. burnetii, sera from mice experimentally infected with nine mile phase i were tested by immunoblotting. the mouse sera recognized antigens with a variety of molecular weights, including proteins of 14, 22, 28, 34, and 60 kda as immunodominant antigens. in order to clone potential protective antigens, a gen ... | 2003 | 12860683 |
| comparison of the efficacy of q fever vaccines against coxiella burnetii experimental challenge in pregnant goats. | | 2003 | 12860684 |
| excretion of coxiella burnetii during an experimental infection of pregnant goats with an abortive goat strain cbc1. | | 2003 | 12860685 |
| fusogenicity of the coxiella burnetii parasitophorous vacuole. | this study investigated whether c. burnetii protein synthesis and replication is required for maintenance of the fusogenic character of the coxiella parasitophorous vacuole (pv). vero cells were infected with c. burnetii, (nine mile strain in phase ii) at a multiplicity of infection of approximately 10 and simultaneously treated with bacteriostatic concentrations of chloramphenicol or carbenicillin. at 96 h post-infection, cells were viewed by phase contrast microscopy for pv maturation. mature, ... | 2003 | 12860689 |
| structural properties of lipopolysaccharides from coxiella burnetii strains henzerling and s. | | 2003 | 12860690 |
| identification of coxiella burnetii rpos-dependent promoters. | | 2003 | 12860695 |
| molecular pathogenesis of coxiella burnetii in a genomics era. | the agent of acute and chronic q fever, coxiella burnetii, occupies a unique niche among intracellular pathogens. the mechanisms the organism employs to cause disease are unclear but involve persistence in a parasitophorous vacuole and the subsequent host response. studies designed to model molecular mechanisms of pathogenesis have relied upon indirect evidence for testing the role of virulence factors since methods for generation of defined mutations have not been developed. evidence suggests r ... | 2003 | 12860703 |
| do chromosomal deletions in the lipopolysaccharide biosynthetic regions explain all cases of phase variation in coxiella burnetii strains? an update. | | 2003 | 12860704 |
| comparative dna microarray analysis of host cell transcriptional responses to infection by coxiella burnetii or chlamydia trachomatis. | dna microarray analysis was conducted to investigate the transcriptional responses of the human monocytic leukemia cell line thp-1 to infection by chlamydia trachomatis or coxiella burnetii. rna was isolated from mock infected cells and cells infected for 36 hours using trizol reagent. biotinylated probes synthesized from rna samples were hybridized to an affymetrix u133a human genome chip consisting of 18,462 human gene probe sets. a total of 335 and 548 thp-1 genes were up- or downregulated at ... | 2003 | 12860710 |
| pathogenic rickettsiae as bioterrorism agents. | the diseases caused by rickettsiae vary from mild to severe clinical presentations, with case fatality ranging from none to over 30%. the severity of rickettsial diseases has been associated with age, delayed diagnosis, hepatic and renal dysfunction, central nervous system abnormalities, and pulmonary compromise. despite the variability in clinical presentations many pathogenic rickettsiae cause debilitating diseases, any one of which could be used as a potential biological weapon. while rickett ... | 2003 | 12860715 |
| probabilistic evaluation of aspirating doses of a q fever pathogen. | the process of biological aerosol penetration into respiratory organs is connected with the estimation of the amount of an aspiration dose of microorganisms (d) a human gets in the center of infection. here we submit a probabilistic model describing the process of hits of a q fever pathogen in a human respiratory tract. this approach makes it possible to get qualitatively different probabilistic estimations of doses received and corresponding values of critical time intervals of exposure at whic ... | 2003 | 12860717 |
| coxiella burnetii seropositivity in parturient women is associated with adverse pregnancy outcomes. | we conducted a cohort study of parturient women in an area with endemic q fever infection to determine whether those seropositive for coxiella burnetii had evidence of adverse birth outcomes. | 2003 | 12861167 |
| scid mouse model for lethal q fever. | q fever, a worldwide zoonosis caused by coxiella burnetii, has many manifestations in humans. endocarditis is the most serious complication of q fever. animal models are limited to acute pulmonary or hepatic disease and reproductive disorders. an appropriate experimental animal model for q fever endocarditis does not yet exist. in this study, severe combined immunodeficient (scid) mice infected with c. burnetii showed persistent clinical symptoms and died, whereas immunocompetent mice similarly ... | 2003 | 12874353 |
| molecular characterization of the sucb gene encoding the immunogenic dihydrolipoamide succinyltransferase protein of bartonella vinsonii subsp. berkhoffii and bartonella quintana. | members of the genus bartonella have historically been connected with human disease, such as cat scratch disease, trench fever, and carrion's disease, and recently have been recognized as emerging pathogens causing other clinical manifestations in humans. however, because little is known about the antigens that elicit antibody production in response to bartonella infections, this project was undertaken to identify and molecularly characterize these immunogens. immunologic screening of a bartonel ... | 2003 | 12874367 |
| coxiella burnetii express type iv secretion system proteins that function similarly to components of the legionella pneumophila dot/icm system. | coxiella burnetii is an obligate intracellular pathogen that replicates in large endocytic vacuoles. genomic sequence data indicate that 21 genes encoding products that are similar to components of the legionella pneumophila dot/icm type iv secretion system are located on a contiguous 35 kb region of the coxiella chromosome. it was found that several dot/icm genes were expressed by coxiella during host cell infection and that dot/icm gene expression preceded the formation of large replicative va ... | 2003 | 12890021 |
| [seroepidemiological studies of zoonotic infections in hunters in southeastern austria--prevalences, risk factors, and preventive methods]. | the aim of this study was to investigate the seroprevalences to zoonotic pathogens in hunters, to propose preventive measures and to obtain more information about the occurrence of zoonotic pathogens in local wild animal populations. from 146 male and 3 female hunters originating from the south-eastern austrian federal states of styria and burgenland blood samples were taken and anamnestic data were obtained using a questionnaire. the serological investigations included the following viral, bact ... | 2003 | 12894685 |
| [analysis of animal material]. | | 2003 | 12905959 |
| experimental coxiella burnetii infection in pregnant goats: excretion routes. | q fever is a widespread zoonosis caused by coxiella burnetii. infected animals, shedding bacteria by different routes, constitute contamination sources for humans and the environment. to study coxiella excretion, pregnant goats were inoculated by the subcutaneous route in a site localized just in front of the shoulder at 90 days of gestation with 3 doses of bacteria (10(8), 10(6) or 10(4) i.d.). all the goats aborted whatever the dose used. coxiella were found by pcr and immunofluorescence tests ... | 2003 | 12911859 |
| active self-splicing group i introns in 23s rrna genes of hyperthermophilic bacteria, derived from introns in eukaryotic organelles. | group i introns are common in the 23 rrna genes of mitochondria and chloroplasts. often, they encode "homing endonucleases," which target highly conserved gene sequences and drive interorganellar intron mobility, even across species and genus lines. most bacterial 23s rrna genes show these same endonuclease-sensitive target sequences. however, only two bacterial 23s rrna genes are known to contain group i introns: that of simkania negevensis [everett, k. d., kahane, s., bush, r. m. & friedman, m ... | 2003 | 12947037 |
| placentitis due to coxiella burnetii in a pacific harbor seal (phoca vitulina richardsi). | | 1999 | 12968740 |
| ovine manure used a a garden fertiliser as a suspected source of human q fever. | | 2003 | 12974339 |
| [frequency of antibodies against r. burneti in tannery personnel]. | | 1952 | 12977982 |
| [rickettsial diseases do not exist in an endemic state in tropical forests as proved by the behavior of murine, boutonneuse, q fever and epidemic rickettsial antigens among the pygmies of the lobaye, oubangui-chari, french equatorial africa]. | | 1952 | 12979226 |
| [cytology of bronchial and alveolar secretions during experimental coxiella burnetti infection in white rat]. | | 1952 | 12982621 |
| [efficiency of our pasteurizing methods in destruction of the q fever coxiella in cows milk]. | | 1952 | 12986758 |