| endocrine and metabolic responses of anguilla anguilla l. caged in a freshwater-wetland (pateira de fermentelos--portugal). | the present short-term in situ study was carried out in a freshwater-wetland--pateira de fermentelos--considered an enlargement of cértima river, in the centre of portugal. this ecosystem is an important fishing and recreation place, receiving effluents from different origins namely, electroplating industrial effluents containing heavy metals, domestic wastes, as well as pesticides and fertilizers resulting from agriculture activities. the aim of the present research work was to monitor the effe ... | 2007 | 17150242 |
| manipulation of dna through ce(iv)/edta-induced site-selective hydrolysis using phosphate-bearing oligonucleotides. | by using two oligonucleotides bearing a monophosphate group at the terminus and placing them at both edges of gap-site formed in substrate dna, this site was preferentially and efficiently hydrolyzed by ce(iv)/edta. in this study, site-selective hydrolysis of gap-site in long substrate dna (274-mer) was investigated. when two monophosphate groups were placed to both edges of 5-base gap, predetermined site was selectively hydrolyzed by ce(iv)/edta. furthermore, desired recombinant dna was prepare ... | 2004 | 17150523 |
| gene manipulation of fluorescent protein through site-selective hydrolysis by ce(iv)/edta. | previously we reported that gap-site, formed in substrate dna by using two oligonucleotide additives, was selectively hydrolyzed by ce(iv)/edta. herein this site-selective scission was used for gene manipulation, and green fluorescent protein (gfp) was converted to blue fluorescent protein (bfp). the sense strand of gfp was cleaved at predetermined site by using this system, and its upstream fragment was connected with the downstream of bfp gene by t4 dna ligase. in this manipulation, three amin ... | 2005 | 17150743 |
| production of circular form of streptavidin rna aptamer in vitro. | rna aptamer is one of functional rnas selected experimentally from randomized rna pools, and has a high affinity and specificity against the target such as a protein and a small ligand. for therapeutic use of rna aptamer in vivo, both no side effects and escaping from rnase-induced degradation may be required. here we show a first evidence of producing a circular streptavidin aptamer in vitro generated by self-splicing permutated intron-exon (pie) sequences derived from gene td of t4 bacteriopha ... | 2006 | 17150948 |
| primary signet-ring cell carcinoma of the colon and rectum: report of eight cases and review of 154 japanese cases. | primary signet-ring cell colorectal carcinoma is rare and has been reported to have an extremely poor prognosis. the purpose of the present study was to define the characteristics of this cancer. | 2006 | 17153438 |
| intraoperative frozen section margin evaluation in gastric cancer of the cardia surgery. | the use of intraoperative frozen section to determine the extent of margin resection is a common practice. our aim was to identify the value of intraoperative frozen section in margin evaluation and to investigate its indication for gastric cancer of the cardia. | 2006 | 17153467 |
| a double-blind randomized placebo-controlled trial with short-term beta-glucuronidase therapy in children with chronic rhinoconjunctivitis and/or asthma due to dust mite allergy. | enzyme potentiated desensitization, in which beta-glucuronidase (bg) is administered with low doses of mixed allergens, was proposed in the 1970s for specific immunotherapy. the bg currently commercially available in a purified and standardized preparation devoid of any allergen has been suggested as a regulator in the allergic immune response, acting on the cytokine-network of type 2 helper t cells. a double-blind trial with a single-dose of bg proved effective in preventing symptoms in adult p ... | 2006 | 17153881 |
| dioxins and related compounds in albatrosses from the torishima island, japan: accumulation features by growth stage and toxicological implications. | concentrations of dioxins and related compounds (drcs), such as polychlorinated dibenzo-p-dioxins (pcdds), polychlorinated dibenzofurans (pcdfs), and coplanar polychlorinated biphenyls (co-pcbs), were determined in eggs, nestlings, and adults of black-footed albatross (bfa; diomedea nigripes) and short-tailed albatross (sta; diomedea albatrus) collected from the torishima island in japan during 2002, which is one of their breeding grounds. observed drc concentrations, especially pcdfs and non-or ... | 2006 | 17153995 |
| [graves' disease in the elderly]. | characterization of elderly (> or = 65) patients with graves' disease (gd) was discussed. emaciation was the symptom that was most frequently found in elderly patients. the presence of goiter, exophthalmos and increased appetite decreased with age, while weight loss, anorexia and arrhythmia increased. elderly patients often have serious complications such as congestive heart failure and atrial fibrillation. serum levels of free t3, free t4 and tsh receptor antibodies were significantly lower in ... | 2006 | 17154098 |
| melting curve of 4he: no sign of a supersolid transition down to 10 mk. | we have measured the melting curve of 4he in the temperature range from 10 to 400 mk with the accuracy of about 0.5 micro bar. crystals of different quality show the expected t4 dependence in the range from 80 to 400 mk without any sign of the supersolid transition, and the coefficient is in excellent agreement with available data on the sound velocity in liquid 4he and on the debye temperature of solid 4he. below 80 mk, we have observed a small deviation from t4 dependence, which, however, cann ... | 2006 | 17155407 |
| [a comparison of t3 sympathectomy versus t4 sympathectomy in the treatment of primary palmer hyperhidrosis]. | to evaluate the effect and side effect of t(3) versus t(4) sympathectomy in the surgical management of primary palmar hyperhidrosis through prospective multi-center randomized controlled trial. | 2006 | 17156625 |
| assembly of acetylcholinesterase tetramers by peptidic motifs from the proline-rich membrane anchor, prima: competition between degradation and secretion pathways of heteromeric complexes. | the membrane-bound form of acetylcholinesterase (ache) constitutes the major component of this enzyme in the mammalian brain. these molecules are hetero-oligomers, composed of four ache catalytic subunits of type t (ache(t)), associated with a transmembrane protein of type 1, called prima (proline-rich membrane anchor). prima consists of a signal peptide, an extracellular domain that contains a proline-rich motif (14 prolines with an intervening leucine, p4lp10), a transmembrane domain, and a cy ... | 2007 | 17158452 |
| local and regional control in patients with papillary thyroid carcinoma: specific indications of external radiotherapy and radioactive iodine according to t and n categories in ajcc 6th edition. | to identify indications for external radiotherapy (ext) and radioactive iodine (rai) in papillary thyroid carcinoma (ptc), we conducted a retrospective study of local and regional control in 1297 patients diagnosed with ptc in a tertiary referral center. managed by surgery alone, patients with bilateral thyroidectomy had a lower rate of local relapse compared with lobectomy (p=0.02). ext improved locoregional (lr) failure-free survival (ffs) (p<0.001) and survival (p=0.01) in patients with gross ... | 2006 | 17158761 |
| cushing's disease arising from a clinically nonfunctioning pituitary adenoma. | a 49-yr-old woman with a large pituitary tumor leading to visual loss and galactorrhea- amenorrhea was submitted to transcranial pituitary surgery, when a clinically nonfunctioning pituitary adenoma was partially removed. histopathology and immunohistochemistry confirmed the diagnosis of "non-secreting atypical adenoma." at that time, serum and urinary free cortisol were normal, with low t4 levels and hyperprolactinemia. the patient was discharged on thyroxine and bromocriptine and treated with ... | 2006 | 17159252 |
| aspiration rate following nonsurgical therapy for laryngeal cancer. | the aim of this study was to evaluate the aspiration rate following nonsurgical therapy, i.e. chemoradiation or radiation alone for laryngeal cancer. modified barium swallow was performed in 43 patients who complained of dysphagia following chemoradiation (n = 22) or radiation alone (n = 21) for laryngeal cancer. patients were selected if they were cancer free at the time of the swallowing study. dysphagia severity was graded on a scale of 1-7. patients were grouped according to the dysphagia se ... | 2007 | 17159375 |
| potential influence of hormones in the development of slipped capital femoral epiphysis: a preliminary study. | the potential influence of hormonal imbalance on the development of slipped capital femoral epiphysis was assessed through a prospective clinical study. the serum levels of t3, t4, thyroid-stimulating hormone, testosterone, estradiol, dehydroepiandrosterone-sulfate, follicle-stimulating hormone, luteinizing hormone, human growth hormone, adrenal cortex hormone and cortisol were evaluated in seven boys and seven girls. forty-three out of 154 hormonal determinations (27.9%) were abnormal. the resu ... | 2007 | 17159524 |
| fluid challenge in patients submitted to spinal block. | the practice of routinely prehydrating patients by infusing a colloid solution for prevention of spinal anesthesia-induced hypotension has been challenged recently. the aim of the study was to evaluate the influence of a 15 ml/kg pre-emptive bolus of colloids (6% hydroxyhethyl starch) on heart rate (hr), mean arterial blood pressure (map), cardiac index (ci) in patients submitted to subarachnoid block with hyperbaric bupivacaine 0.5%, 0.2 mg/kg for orthopedic surgery of the lower limb. | 2007 | 17159758 |
| thoracoscopy in children: anaesthesiological implications and case reports. | videoassisted thoracic surgical technique in children is being used with increasing frequency for an extensive variety of diagnostic and therapeutic procedures. the aim of the study was to assess respiratory, cardiocirculatory and body temperature changes in children undergoing thoracoscopy and to identify if the trend of such changes was modifiable by factors such as lung exclusion, length of the thoracoscopy and preoperative respiratory compromise. | 2007 | 17159760 |
| [navigated "targeted surgery" for skull base malignomas: additional space for surgical manipulation by neoadjuvant tumor downsizing]. | multimodal strategies are required due to the poor prognosis for locally advanced frontal skull base tumors staged as t4. therefore, a further increase in the degree of invalidity caused by therapy should be avoided, if possible, to preserve the quality of life. as the incidence of these tumors is low, there are no evidence-based, generally accepted therapeutic strategies. | 2007 | 17160663 |
| treatment of nasal cavity and paranasal sinus cancer with modern radiotherapy techniques in the postoperative setting--the mskcc experience. | to perform a retrospective analysis of patients with paranasal sinus (pns) cancer treated with postoperative radiotherapy (rt) at memorial sloan-kettering cancer center. | 2007 | 17161557 |
| concurrent chemoradiation for locally advanced oropharyngeal cancer. | the aim of this study was to assess the survival, pattern of failure, morbidity, and prognostic factors of concurrent chemoradiation for locally advanced oropharyngeal cancer. | 2007 | 17162122 |
| the effects of isoflurane, sevoflurane, and desflurane anesthesia on neurocognitive outcome after cardiac surgery: a pilot study. | inhalation anesthetics such as isoflurane, sevoflurane, and desflurane are widely used in clinical practice; however, there is no study for comparing these drugs in cardiac surgery with respect to postoperative cognitive outcome and s100 beta protein (s100 bp) levels. in this study, we evaluated the effect of sevoflurane, isoflurane, and desflurane anesthesia on neuropsychological outcome and s100 bp levels in patients undergoing coronary artery bypass grafting (cabg) surgery with cardiopulmonar ... | 2007 | 17162400 |
| counseling patients receiving levothyroxine (l-t4) and calcium carbonate. | | 2006 | 17162777 |
| treatment of oropharyngeal squamous cell carcinoma with external beam radiation combined with interstitial brachytherapy. | we reviewed the outcomes of oropharyngeal squamous cell carcinoma treated with external beam radiation and interstitial brachytherapy. | 2007 | 17163468 |
| in vitro selection and characterization of rna aptamers binding thyroxine hormone. | rna possesses the ability to bind a wide repertoire of small molecules. some of these binding interactions have been shown to be of primary importance in molecular biology. for example, several classes of mrna domains, collectively referred to as riboswitches, have been shown to serve as rna genetic control elements that sense the concentrations of specific metabolites (i.e. acting as direct sensors of chemical compounds). however, to date no rna species binding a hormone has been reported. here ... | 2007 | 17163839 |
| stable transgene expression and random gene silencing in wheat. | wheat genes for pathogenesis-related (pr-)proteins, chitinase and beta-1,3-glucanase, under the control of maize ubiquitin promoter-intron were used for transforming the spring wheat 'bobwhite', using a biolistic approach. twenty of the 24 primary transgenic lines expressing the pr-protein genes in the t0 generation were silenced in either the t1 or t2 generations. two apparently genetically identical regenerants arising from a single callus co-bombarded with chitinase and beta-1,3-glucanase tra ... | 2003 | 17163901 |
| from structure of the complex to understanding of the biology. | the most extensive structural information on viruses relates to apparently icosahedral virions and is based on x-ray crystallography and on cryo-electron microscopy (cryo-em) single-particle reconstructions. both techniques lean heavily on imposing icosahedral symmetry, thereby obscuring any deviation from the assumed symmetry. however, tailed bacteriophages have icosahedral or prolate icosahedral heads that have one obvious unique vertex where the genome can enter for dna packaging and exit whe ... | 2007 | 17164521 |
| pro-angiogenesis action of thyroid hormone and analogs in a three-dimensional in vitro microvascular endothelial sprouting model. | our laboratory has recently demonstrated the pro-angiogenesis effects of thyroid hormone in the chick chorioallantoic membrane model. | 2006 | 17164749 |
| [observation on therapeutic effect of picking therapy on graves' disease]. | to observe therapeutic effect of picking therapy on graves' disease (gd) and its effects on thyroid function. | 2006 | 17165495 |
| [histologic response after neoadjuvant therapy in rectal adenocarcinoma: own experience and review of the literature]. | neoadjuvant therapy has improved outcomes for patients with locally advanced rectal cancer. aim authors: we studied the degree of histopathological regression after radiotherapy regimens commonly used in their clinical practice, and its effect on the pathology of by clinically founded t3-t4 rectal cancer. | 2006 | 17165600 |
| smoking is associated with worse outcomes in patients with prostate cancer treated by radical radiotherapy. | to investigate the effect of smoking on the outcome in a cohort of men treated for localized prostate cancer at one institution with a uniform protocol of radical external beam radiotherapy (ebrt). | 2007 | 17166241 |
| [regulatory expression of human interferon-gamma gene in murine bone marrow stromal cells by tet-off system]. | the tet-off system is a gene expression regulating system, which has high efficiency, low toxicity and strict turning-off function. this study was to investigate the regulatory expression of human interferon-gamma (ifngamma) gene in murine bone marrow stromal cells (bmscs) by tet-off system. | 2006 | 17166390 |
| molecular modeling of the thyroid hormone interactions with alpha v beta 3 integrin. | a cell surface receptor for thyroid hormone has recently been identified on the extracellular domain of integrin alphavbeta3. in a variety of human and animal cell lines this hormone receptor mediates activation by thyroid hormone of the cellular mitogen-activated protein kinase (mapk) signal transduction cascade. an arginine-glycine-aspartate (rgd) recognition site on the heterodimeric integrin is essential to the binding of a variety of extracellular matrix proteins. recent competition data re ... | 2007 | 17166537 |
| [breast cancer with noninflammatory skin involvement: new data revise the traditional image of a "classical" clinicopathologic entity]. | historically, the presence of skin involvement results in the classification of a breast carcinoma into the highest tumor category, or accordingly into the highest non-metastatic disease stage (current tnm classification: t4/stage iii). however, probably the most important criterion of the tnm classification system is the basic rule that tumors exhibiting similar extension and prognosis should be grouped into one category. newer studies indicate the need to revise the current tnm system, since g ... | 2006 | 17168078 |
| selenium derivatization of nucleic acids for crystallography. | the high-resolution structure of the dna (5'-gtgtaca-c-3') with the selenium derivatization at the 2'-position of t2 was determined via mad and sad phasing. the selenium-derivatized structure (1.28 a resolution) with the 2'-se modification in the minor groove is isomorphorous to the native structure (2.0 a). to directly compare with the conventional bromine derivatization, we incorporated bromine into the 5-postion of t4, determined the bromine-derivatized dna structure at 1.5 a resolution, and ... | 2007 | 17169989 |
| chronic local inflammation in mice results in decreased trh and type 3 deiodinase mrna expression in the hypothalamic paraventricular nucleus independently of diminished food intake. | during illness, changes in thyroid hormone metabolism occur, known as nonthyroidal illness and characterised by decreased serum triiodothyronine (t3) and thyroxine (t4) without an increase in tsh. a mouse model of chronic illness is local inflammation, induced by a turpentine injection in each hind limb. although serum t3 and t4 are markedly decreased in this model, it is unknown whether turpentine administration affects the central part of the hypothalamus-pituitary-thyroid axis (hpt-axis). we ... | 2006 | 17170227 |
| management of unresectable malignant tumors at the skull base using concomitant chemotherapy and radiotherapy with accelerated fractionation. | between january 1988 and june 1992, 20 patients with unresectable malignant tumors at the skull base were treated. eleven had t4 lesions of the paranasal sinus/cavity complex, and 9 had t4 nasopharynx cancer. all patients had stage iv disease by the american joint committee on staging criteria. the histology was squamous cell cancer in 15 patients and other minor salivary gland histologies in 5. there was brain and/or dural invasion in 11 patients and orbital invasion in 9. all patients received ... | 1994 | 17171161 |
| prostate cancer progression in the presence of undetectable or low serum prostate-specific antigen level. | the serum prostate-specific antigen (psa) level after definitive treatment for prostate cancer (pc) is a powerful predictor of outcome. occasionally, pc progression can occur despite low or undetectable psa levels. the authors report on the clinical and pathologic characteristics of patients who experienced pc progression with undetectable or low psa levels. | 2007 | 17171704 |
| expression in human ocular surface tissues of the galnac-transferases that initiate mucin-type o-glycosylation. | mucins are highly glycosylated proteins that act as lubricants, protectants, and mediators of signal transduction. the udp-galnac:polypeptide n-acetylgalactosaminyltransferase (ppgantase) family members initiate mucin-type o-glycosylation. because o-glycosylation provides mucins with the viscoelastic properties required for proper mucin function, ppgantase expression is vital to the maintenance of healthy epithelial surfaces including the ocular surface. differences of expression of ppgantase is ... | 2006 | 17172897 |
| effect of a hypoglycemic agent on ischemic preconditioning in patients with type 2 diabetes and stable angina pectoris. | ischemic preconditioning is an increased tolerance to myocardial ischemia during the second of two consecutive exercise tests. atp-sensitive k(+) channel blockers, such as glinides and sulfonylurea drugs, can induce loss of ischemic preconditioning. this study aimed to investigate the effects of repaglinide, a hypoglycemic agent with an affinity for myocardial atp-sensitive k (+)channels, on the results of consecutive exercise tests in patients with diabetes and multivessel coronary artery disea ... | 2007 | 17172931 |
| effect of selenium supplementation on biochemical markers and outcome in critically ill patients. | this study aimed to assess the effect of high dose selenium (se) supplementation on se status in blood, oxidative stress, thyroid function and possible effects on requirement for renal replacement therapy (rrt) in severely septic patients admitted to the intensive care unit (icu). | 2007 | 17174015 |
| possible relations between thyroid function, endothelium, and kidney and liver function in kidney allograft recipients. | renal function affects the thyroid gland in many ways. disturbances in hemostasis and endothelial damage are common complications of kidney disease. endothelial dysfunction may link these two processes. | 2006 | 17175317 |
| primary radiotherapy for tonsillar carcinoma: a good alternative to a surgical approach. | to review the notre-dame hospital experience in the treatment of tonsillar carcinoma with primary radiotherapy and to evaluate the different factors affecting locoregional control (lrc) and survival. | 2006 | 17176797 |
| application of microbial risk assessment on a residentially-operated bio-toilet. | the sustainable sanitation system is a new wastewater treatment system that incorporates a non-flushing toilet (bio-toilet) that converts excreta into a reusable resource (as fertilizer or humus for organic agriculture) and reduces the pollution load to environments of the rivers, the lakes, and the sea. however, the risk of exposure to pathogens should be considered, because excrement is stored in the bio-toilet. the aim of the present work is to analyze the health risk of dealing with the matr ... | 2006 | 17176818 |
| a novel mutation (e333d) in the thyroid hormone beta receptor causing resistance to thyroid hormone syndrome. | resistance to thyroid hormone (rth) is an inherited syndrome characterized by elevated serum thyroid hormones (th), failure to suppress pituitary thyroid stimulating hormone (tsh) secretion, and variable peripheral tissue responsiveness to th. the disorder is associated with diverse mutations in the thyroid hormone beta receptor (trbeta). here, we report a novel natural rth mutation (e333d) located in the large carboxy-terminal ligand binding domain of trbeta. the mutation was identified in a 22 ... | 2006 | 17177139 |
| transcriptional profiles in liver from rats treated with tumorigenic and non-tumorigenic triazole conazole fungicides: propiconazole, triadimefon, and myclobutanil. | conazoles are a class of fungicides used as pharmaceutical and agricultural agents. in chronic bioassays in rats, triadimefon was hepatotoxic and induced follicular cell adenomas in the thyroid gland, whereas, propiconazole and myclobutanil were hepatotoxic but had no effect on the thyroid gland. these conazoles administered in the feed to male wistar/han rats were found to induce hepatomegaly, induce high levels of pentoxyresorufin-o-dealkylase, increase cell proliferation in the liver, increas ... | 2006 | 17178689 |
| toxicity profiles in rats treated with tumorigenic and nontumorigenic triazole conazole fungicides: propiconazole, triadimefon, and myclobutanil. | conazoles are a class of azole based fungicides used in agriculture and as pharmaceutical products. they have a common mode of antifungal action through inhibition of ergosterol biosynthesis. some members of this class have been shown to be hepatotoxic and will induce mouse hepatocellular tumors and/or rat thyroid follicular cell tumors. the particular mode of toxic and tumorigenic action for these compounds is not known, however it has been proposed that triadimefon-induced rat thyroid tumors a ... | 2006 | 17178690 |
| prognostic groups in 1,676 patients with t3 rectal cancer treated without preoperative radiotherapy. | the use of preoperative radiotherapy in patients with t3 tumors shows considerable variation among countries and institutions. the norwegian guidelines have been very restrictive, limiting the indication to t4. this study was designed to identify subgroups of patients with t3 tumors with presumed high risks on adverse outcome and to use these results to reevaluate the national guidelines for preoperative radiotherapy. | 2007 | 17180256 |
| short-term outcome after laparoscopic or open restorative mesorectal excision for rectal cancer: a comparative cohort study. | the laparoscopic approach to rectal cancer is still a controversial procedure. a comparative cohort study was conducted to assess short-term results of laparoscopic restorative mesorectal excision. | 2007 | 17180257 |
| analysis of anatomic morphometry of the pedicles and the safe zone for through-pedicle procedures in the thoracic and lumbar spine. | posterior instrumentation through the pedicle is a common surgery. understanding the morphometry of the pedicle and the anatomy of adjacent neural structures should help decrease the risk of postoperative complications. t1-l5 segments from 15 sets of human vertebrae were separated into individual vertebrae and the morphometric characteristics of the thoracic and lumbar spine and the safe zone of the pedicle were analyzed. t11-l5 segments from six human cadavers were dissected. measurements were ... | 2007 | 17180401 |
| modeling the effects of structure and dynamics of the nitroxide side chain on the esr spectra of spin-labeled proteins. | in the companion paper (j. phys. chem. b 2006, 110, jp0629487), a study of the conformational dynamics of methanethiosulfonate spin probes linked at a surface-exposed alpha-helix has been presented. here, on the basis of this analysis, x-band esr spectra of these spin labels are simulated within the framework of the stochastic liouville equation (sle) methodology. slow reorientations of the whole protein are superimposed on fast chain motions, which have been identified with conformational jumps ... | 2006 | 17181284 |
| urinary perchlorate and thyroid hormone levels in adolescent and adult men and women living in the united states. | perchlorate is commonly found in the environment and known to inhibit thyroid function at high doses. assessing the potential effect of low-level exposure to perchlorate on thyroid function is an area of ongoing research. | 2006 | 17185277 |
| [adenocarcinomas of the ethmoid sinus: retrospective analysis of prognostic factors]. | determinate the different prognostic factors of survival in ethmoidal sinus adenocarcinomas (adk). | 2006 | 17185918 |
| [carcinoma of the transverse colon]. | carcinoma of the transverse colon accounts for 10% of all colorectal cancer. diagnosis is often delayed and complicated forms (perforation, fistulization, obstruction) occur in 30-50% of cases. the progression of symptoms is often insidious and tumors may be voluminous by the time of diagnosis. right sided tumors are typically bulky and late-stage; perforation may lead to abdominal wall or retroperitoneal abscess. tumor can also extend or fistulize into adjacent organs. distal transverse cancers ... | 2006 | 17185954 |
| a type iv modification dependent restriction nuclease that targets glucosylated hydroxymethyl cytosine modified dnas. | the escherichia coli ct596 prophage exclusion genes gmrs and gmrd were found to encode a novel type iv modification-dependent restriction nuclease that targets and digests glucosylated (glc)-hydroxymethylcytosine (hmc) dnas. the protein products gmrs (36 kda) and gmrd (27 kda) were purified and found to be inactive separately, but together degraded several different glc-hmc modified dnas (t4, t2 and t6). the gmr enzyme is able to degrade both alpha-glucosy-hmc t4 dna and beta-glucosyl-hmc t4 dna ... | 2007 | 17188297 |
| exclusion of glucosyl-hydroxymethylcytosine dna containing bacteriophages is overcome by the injected protein inhibitor ipi*. | the escherichia coli isolate ct596 excludes infection by the myoviridae t4 ip1(-) phage that lacks the encapsidated ipi* protein normally injected into the host with the phage dna. screening of a ct596 genomic library identified adjacent genes responsible for this exclusion, gmrs (942 bp) and gmrd (708 bp) that are encoded by a cryptic prophage dna. the two genes are necessary and sufficient to confer upon a host the ability to exclude infection by t4 ip1(-) phage and other glucosyl-hydroxymethy ... | 2007 | 17188711 |
| hydrolytic cleavage of dna by quercetin zinc(ii) complex. | quercetin zinc(ii) complex was investigated focusing on its hydrolytic activity toward dna. the complex successfully promotes the cleavage of plasmid dna, producing single and double dna strand breaks. the amount of conversion of supercoiled form (sc) of plasmid to the nicked circular form (nc) depends on the concentration of the complex as well as the duration of incubation of the complex with dna. the rate of conversion of sc to nc is 1.68x10(-4) s(-1) at ph 7.2 in the presence of 100 microm o ... | 2007 | 17189687 |
| natural triple beta-stranded fibrous folds. | a distinctive family of beta-structured folds has recently been described for fibrous proteins from viruses. virus fibers are usually involved in specific host-cell recognition. they are asymmetric homotrimeric proteins consisting of an n-terminal virus-binding tail, a central shaft or stalk domain, and a c-terminal globular receptor-binding domain. often they are entirely or nearly entirely composed of beta-structure. apart from their biological relevance and possible gene therapy applications, ... | 2006 | 17190612 |
| simulation of dna electrophoresis through microstructures. | the dependence of the mobility of dna molecules through an hexagonal array of micropillars on their length and the applied electric field was investigated and it was found that mobility is a nonmonotonic function of their length. results also revealed that the size dependence of the dna mobility depends on the applied electric field and there is a crossover around e approximately 25 v/cm for the mobility of lambda-dna and t4-dna. these observations are explained in terms of the diffusion process ... | 2007 | 17191278 |
| differing effects of t4 dna ligase in the modulation of the damage induced in mammalian cells by either x-rays or restriction endonucleases. | of the different lesions induced by x-rays, dna double-strand breaks (dsbs) are considered the main cause of chromosomal aberrations and cell death. restriction endonucleases (res) induce only dna dsbs and have frequently been used to mimic the effects of ionizing radiation in the study of dna damage and repair. | 2007 | 17192708 |
| an evaluation of thyroid hormone status and oxidative stress in undialyzed chronic renal failure patients. | chronic renal failure (crf) patients on prolonged dialysis have been found to have significant alteration in their thyroid status, but little is known about the same in undialyzed crf patients. oxidative stress has been implicated as the key player in altering the levels of thyroid hormone in euthyroid sick syndrome. this study was performed to evaluate the levels of oxidative stress and thyroid status in undialyzed crf patients. a case control study was performed on 20 undialyzed crf patients a ... | 2006 | 17193900 |
| proteomic profiling of cold thyroid nodules. | cold thyroid nodules (ctns) represent a frequent endocrine disorder accounting for up to 85% of thyroid nodules in a population living in an iodine-deficient area. benign ctns need to be distinguished from thyroid cancer, which is relatively rare. the molecular etiology of benign ctns is unresolved. to obtain novel insights into their pathogenesis, protein expression profiling was performed in a series of 27 solitary ctns (10 follicular adenoma and 20 adenomatous nodules) and surrounding normal ... | 2007 | 17194741 |
| multicenter phase ii trial of chemoradiation with oxaliplatin for rectal cancer. | to evaluate the activity and safety of preoperative radiotherapy (rt) and concurrent capecitabine and oxaliplatin (xelox-rt) plus four cycles of adjuvant xelox in patients with rectal cancer. | 2007 | 17194912 |
| effect of selenium on thyroid hormone metabolism in filial cerebrum of mice with excessive iodine exposure. | the effects of supplementing selenium on thyroid hormone metabolism were studied on mice with excessive iodine exposure. the serum concentrations of thyroxine (t4) and triiodothyronine (t3) and the activities of iodothyronine 5\' and 5-deiodinase (d2, d3) were measured in the brain of filial mice to study the influence of selenium on thyroid hormone metabolism. measurements were carried out on postnatal day 0, 14, and 28. it was found that selenium supplementation alleviated the adverse effects ... | 2006 | 17194928 |
| differential dependence on dna ligase of type ii restriction enzymes: a practical way toward ligase-free dna automaton. | dna computing study is a new paradigm in computer science and biological computing fields. as one of dna computing approaches, dna automaton is composed of the hardware, input dna molecule and state transition molecules. by now restriction enzymes are key hardware for dna computing automaton. it has been found that dna computing efficiency may be independent on dna ligases when type iis restriction enzymes like foki are used as hardware. in this study, we compared foki with four other distinct e ... | 2007 | 17196173 |
| effects of larval-juvenile treatment with perchlorate and co-treatment with thyroxine on zebrafish sex ratios. | the objective of this study was to determine the effect of larval-juvenile exposure to perchlorate, a thyroid hormone synthesis inhibitor, on the establishment of gonadal sex ratios in zebrafish. zebrafish were exposed to untreated water or water containing perchlorate at 100 or 250 ppm for a period of 30 days starting at 3 days postfertilization (dpf). recovery treatments consisted of a combination of perchlorate and exogenous thyroxine (t4; 10 nm). thyroid histology was assessed at the end of ... | 2007 | 17196199 |
| cns hypomyelination in rat terrier dogs with congenital goiter and a mutation in the thyroid peroxidase gene. | arrested physical development and neurologic abnormalities were identified in 3 of 5 rat terrier puppies at 9 weeks of age. bilaterally firm symmetrical masses were palpated in the region of the thyroid glands. low serum total (t4) and free thyroxine (ft4, by equilibrium dialysis) and markedly elevated thyroid stimulating hormone (tsh) concentrations supported the diagnosis of hypothyroidism. at necropsy, the thyroid gland was grossly enlarged and histologically exhibited severe, diffuse hyperpl ... | 2007 | 17197623 |
| a prospective pathologic analysis using whole-mount sections of rectal cancer following preoperative combined modality therapy: implications for sphincter preservation. | the aims of this study were to use a comprehensive whole-mount pathologic analysis to characterize microscopic patterns of residual disease, as well as circumferential and distal resection margins, in rectal cancer treated with preoperative cmt; and to identify clinicopathologic factors associated with residual disease. | 2007 | 17197970 |
| continued suppression of serum tsh level may be attributed to tsh receptor antibody activity as well as the severity of thyrotoxicosis and the time to recovery of thyroid hormone in treated euthyroid graves' patients. | the cause of continued suppression of serum thyroid-stimulating hormone (tsh) levels during antithyroid drug therapy in some graves' patients is unclear. recently, there has been a notable explanation involving the direct inhibition of tsh receptor antibody (trab) on tsh secretion in the pituitary gland. the purpose of this study is to verify the relation between trab or other clinical parameters and the continued suppression of serum tsh level during antithyroid drug therapy in patients with gr ... | 2006 | 17199435 |
| l-t4 bioequivalence and hormone replacement studies via feedback control simulations. | fda guidance for testing bioequivalence of levothyroxine (l-t(4)) preparations has been challenged by several groups, based on multiple issues. the efficacy of single versus combined hormone therapy also is receiving additional scrutiny. to examine these concerns, we developed a new nonlinear feedback system simulation model of whole-body regulation mechanisms involving dynamics of t(3), t(4), tsh, plasma protein binding, extravascular regulatory enzyme systems, and the hypothalamic-pituitary-th ... | 2006 | 17199439 |
| [development of a dna chip screening mitochondrial dna mutations in patients with diabetes mellitus]. | to establish a rapid and precise high-throughput mitochondrial (mt) dna chip and to investigate the relationship between mtdna trna leu (uur) and nd1 gene mutations and diabetes mellitus. | 2006 | 17200023 |
| timing of resection of liver metastases synchronous to colorectal tumor: proposal of prognosis-based decisional model. | timing of hepatectomy for synchronous metastases of colorectal cancer is still debated. the aim of this retrospective study was to analyze prognostic factors after synchronous and delayed liver resections to define selection criteria for choosing timing of hepatectomy. | 2007 | 17200913 |
| effects of evening vs morning thyroxine ingestion on serum thyroid hormone profiles in hypothyroid patients. | standard drug information resources recommend that l-thyroxine be taken half an hour before breakfast on an empty stomach, to prevent interference of its intestinal uptake by food or medication. we observed cases in which tsh levels improved markedly after changing the administration time of l-thyroxine to the late evening. we therefore conducted a pilot-study to investigate whether l-thyroxine administration at bedtime improves tsh and thyroid hormones, and whether the circadian rhythm of tsh r ... | 2007 | 17201800 |
| unmasking of central hypothyroidism following growth hormone replacement in adult hypopituitary patients. | the effect of gh replacement on thyroid function in hypopituitary patients has hitherto been studied in small groups of children and adults with conflicting results. | 2007 | 17201804 |
| expression analysis of pancreatic cancer cell lines reveals association of enhanced gene transcription and genomic amplifications at the 8q22.1 and 8q24.22 loci. | despite tremendous effort and progress in the diagnostics of pancreatic cancer with respect to imaging techniques and molecular genetics, only very few patients can be cured by surgery leading to a 5-year survival rate of only 3%. especially the lack of chemotherapeutical options in this entity requires a better understanding of the molecular mechanisms leading to pancreatic carcinoma growth and progression in order to develop novel treatment regimens. to identify signaling pathways that are cri ... | 2007 | 17203180 |
| thyroid hormones concentrations and ecg picture in the dog. | disorders of the thyroid gland activity are the most commonly encountered disturbances of endocrine origin in the dog. hypo- or hyperthyroidism may disturb the function of the cardiovascular system and cause arrhythmias. the aim of this study was to evaluate the influence of thyroid gland activity on electrocardiogram (ecg) picture in the dog by comparing ecg curves of healthy dogs, dogs with hypothyroidism and dogs with cardiac insufficiency caused by endocardiosis of the mitral valve. the stud ... | 2006 | 17203744 |
| expression of maspin in colorectal cancer. | maspin, a member of the serpin family of protease inhibitors, is known to be a tumor suppressor. although the relationship between its expression and biological significance has been investigated in several types of cancer, the clinicopathological significance of maspin expression in colorectal cancer has not been fully elucidated. | 2006 | 17203770 |
| zosteriform skin involvement of nodal t-cell lymphoma: a review of the published work of cutaneous malignancies mimicking herpes zoster. | a 77-year-old japanese woman initially presented with non-hodgkin's lymphoma involving her neck, axillary and inguen lymph nodes. she had edematous erythema and nodules limited to the skin in zosteriform distribution on the left side chest wall along the t4-5 dermatome. in addition, since 1970, we have mainly been collecting english-language articles on malignant skin tumors and skin metastasis described as zosteriform in the title, and we have reviewed a total of 29 cases, including our own. it ... | 2007 | 17204106 |
| factors associated with poor general health after stem-cell transplantation. | to describe functional status (fs), general health (gh) and symptom distress (sd) from admission to 1 year post-sct and to identify medical, demographic, and/or patient-reported outcome variables associated with patient-perceived gh. | 2007 | 17205276 |
| effect of iodine supplement on iodine status and 5'-deiodinase activity in the brain of neonatal rats with iodine deficiency. | the weanling wistar rats of iodine deficiency were divided into three groups for supplementation of different levels of iodine (iodine-excessive [ie], iodine-adequate [ia], and iodine-deficient [id]), with a control group (c). the iodine content in the thyroid was determined by epithermal neutron activation analysis. the activities of 5'-deiodinase and 5-deiodinase in the brains were assayed by determining the conversion ratios of t4 to t3 and rt3, respectively. the thyroid hormones levels in se ... | 2006 | 17206003 |
| angiotensin type 1 (at1) and type 2 (at2) receptors mediate the increase in tgf-beta1 in thyroid hormone-induced cardiac hypertrophy. | increased thyroid hormone (th) levels are known to induce cardiac hypertrophy. some studies have provided evidence for a functional link between angiotensin ii (ang ii) and transforming growth factor beta1 (tgf-beta1) in the heart, both being able to also induce cardiac hypertrophy. however, the contribution of this growth factor activated directly by th or indirectly by ang ii in cardiac hypertrophy development remains unknown. to analyze the possible role of tgf-beta1 in cardiac hypertrophy in ... | 2007 | 17206447 |
| neoadjuvant therapy before radical prostatectomy: where have we been? where are we going? | prostate cancer is curable only when treated at an early stage, when the tumor is still localized to the prostate gland. however, even in apparent cases of clinically localized disease, unsuspected extracapsular disease may significantly increase the risk of primary treatment failure. this risk is especially high if the patient has > or =1 of the following risk factors: a serum prostate-specific antigen level of >20 ng/ml, a gleason score of >7, locally advanced disease (clinical stage t3/t4), a ... | 2007 | 17208133 |
| supra dietary levels of vitamins c and e enhance antibody production and immune memory in juvenile milkfish, chanos chanos (forsskal) to formalin-killed vibrio vulnificus. | juveniles of milkfish, chanos chanos (forsskal), were fed two independent supra dietary levels of vitamins c (500 and 1500 mg kg(-1) feed, t1 and t2) and e (50 and 150 mg kg(-1), t3 and t4). milkfish fed diets with supra (in addition to the vitamins present in the control diet) and normal levels (t5 containing 90 and 1.2mg of vitamins c and e, respectively, kg(-1) of feed) of vitamins were immunized (ip) with formalin-killed vibrio vulnificus (fkvv). priming and booster antibody responses to the ... | 2007 | 17208456 |
| phase i/ii study of preoperative cetuximab, capecitabine, and external beam radiotherapy in patients with rectal cancer. | to assess the safety and preliminary efficacy of concurrent radiotherapy, capecitabine, and cetuximab in the preoperative treatment of patients with rectal cancer. | 2007 | 17208931 |
| dynamics of lysozyme structure network: probing the process of unfolding. | recently we showed that the three-dimensional structure of proteins can be investigated from a network perspective, where the amino acid residues represent the nodes in the network and the noncovalent interactions between them are considered for the edge formation. in this study, the dynamical behavior of such networks is examined by considering the example of t4 lysozyme. the equilibrium dynamics and the process of unfolding are followed by simulating the protein at 300 k and at higher temperat ... | 2007 | 17208969 |
| approach to the patient with subclinical hyperthyroidism. | endogenous subclinical hyperthyroidism, defined by normal circulating levels of free t4 and t3 and low levels of tsh, is a common clinical entity and is typically caused by the same conditions that account for the majority of cases of overt hyperthyroidism: graves' disease, toxic multinodular goiter, and solitary autonomously functioning thyroid nodules. subclinical hyperthyroidism has been associated with an increased risk of atrial fibrillation and mortality, decreased bone mineral density in ... | 2007 | 17209221 |
| targeted intra-arterial carboplatin chemoradiotherapy and tegafur/uracil for oral and oropharyngeal cancer. | the aim of this study was to evaluate the usefulness of targeted intra-arterial carboplatin chemoradiotherapy in allowing less invasive surgery for patients with oral and oropharyngeal squamous cell carcinoma. twenty patients with previously untreated squamous cell carcinoma of the oral cavity and oropharynx (t4; 8, t2; 12 patients) were treated with targeted transfemoral intra-arterial carboplatin infusion with concurrent hyperfractionated radiotherapy and the administration of tegafur/uracil ( ... | 2006 | 17209514 |
| a randomized trial of t3-t4 versus t4 sympathectomy for isolated axillary hyperhidrosis. | video-assisted thoracic sympathectomy (vats) is one minimally invasive definitive treatment for axillary hyperhidrosis. different techniques exist for controlling axillary sudoresis, but they are temporary and have high cost. this study was conducted to compare the initial results from sympathectomy using two distinct levels for treating axillary sudoresis: t3-t4 vs t4. | 2007 | 17210397 |
| sexual dimorphism in thyroid function and type 1 iodothyronine deiodinase activity in pre-pubertal and adult rats. | iodothyronine deiodinase activities are regulated by sex steroids; however, the mechanisms underlying the reported sexual dimorphism are poorly defined. in the present report, we aimed to investigate whether type 1 deiodinase (d1) sexual dimorphism exists early in sexual development by studying pre-pubertal male (pm) and female (pf) rats, as well as adult controls (c) and gonadectomized male and females rats. adult male wistar rats were studied 21 days after orchiectomy (tex), and adult females ... | 2007 | 17210749 |
| [improvements in quality of life and survival of patients with t4 gastric cancer which invaded organs after multivisceral resections through the extension of palliative gastrectomy and post-operative chemotherapy]. | for patients with a locally advanced gastric cancer, palliative gastrectomy could improve local complications, such as bleeding or obstruction, but had no impact on prognosis. this study was designed to evaluate improvements in quality of life and survival of patients with t4 gastric cancer which invaded organs exhibiting bleeding or obstruction, after multivisceral resections of these organs through the extension of palliative gastrectomy and postoperative chemotherapy. multivisceral resections ... | 2006 | 17212135 |
| effect of environmental iodine deficiency (eid) on foetal growth in nigeria. | there is scarcity of information on impact of iodine deficiency on growth and development of newborns, both pre-term and term babies. the present study was carried out to see the impact of iodine deficiency (id) on the intrauterine foetal growth and development in terms of birth weight (bw) in an african population living in an iodine deficient zone. | 2006 | 17213522 |
| safety and efficacy of administering 0.2 mg of recombinant human tsh for two consecutive days as an adjuvant to therapy with low radioiodine doses in elderly out-patients with large nontoxic multinodular goiter. | the aim of this study was to evaluate the efficacy of recombinant human tsh (rhtsh) as an adjuvant to radioiodine therapy for nontoxic multinodular goiter (mng) in elderly subjects. | 2006 | 17213787 |
| intensity-modulated radiation therapy in head-and-neck cancer, first report in thailand. | this is the first report in thailand to evaluate the efficacy of using intensity-modulated radiotherapy (imrt) in the primary treatment of head-and-neck cancer. | 2006 | 17214058 |
| mycosis fungoides--a review of the management of 28 patients and of the recent literature. | mycosis fungoides is an uncommon cutaneous t-cell lymphoma characterized by malignant monoclonal proliferation of t-helper lymphocytes. its course is variable with a potential for lymphatic and hematogenous involvement. we report the investigations, staging, treatment, follow-up, and outcome of 28 patients. this is the first such study reported from ireland. | 2007 | 17214715 |
| the lectin domains of polypeptide galnac-transferases exhibit carbohydrate-binding specificity for galnac: lectin binding to galnac-glycopeptide substrates is required for high density galnac-o-glycosylation. | initiation of mucin-type o-glycosylation is controlled by a large family of udp galnac:polypeptide n-acetylgalactosaminyltransferases (galnac-transferases). most galnac-transferases contain a ricin-like lectin domain in the c-terminal end, which may confer galnac-glycopeptide substrate specificity to the enzyme. we have previously shown that the lectin domain of galnac-t4 modulates its substrate specificity to enable unique galnac-glycopeptide specificities and that this effect is selectively in ... | 2007 | 17215257 |
| insular carcinomas of the thyroid exhibit poor prognosis and long-term survival in comparison to follicular and papillary t4 carcinomas. | insular thyroid carcinoma was described as a tumor with aggressive behavior, and patients usually present themselves with an advanced tumor stage. whether the insular component is an independent factor for poor prognosis remains unclear. therefore, in the present study, we compared the survival of patients with advanced insular, follicular, and papillary thyroid cancer. | 2007 | 17216284 |
| semi-invasive monitoring of cardiac output by a new device using arterial pressure waveform analysis: a comparison with intermittent pulmonary artery thermodilution in patients undergoing cardiac surgery. | thermodilution technique using a pulmonary artery catheter (pac) is a widely used method to determine cardiac output (co). it is increasingly criticized because of its invasiveness and its unclear risk-benefit ratio. thus, less invasive techniques for measuring co are highly desirable. we compared a new, semi-invasive device (flotrac/vigileo) using arterial pressure waveform analysis for co measurement in patients undergoing cardiac surgery with bolus thermodilution measurements. | 2007 | 17218375 |
| effects of epidural anaesthesia on surgical stress-induced immunosuppression during upper abdominal surgery. | previously, we have demonstrated that surgical stress rapidly induced transient hyporesponsiveness of blood cells to endotoxin and that monocyte mcd14 and hla-dr expression decreased soon after the start of surgery under general anaesthesia. this study was designed to investigate the effects of epidural anaesthesia on surgical stress-induced immunosuppression in patients undergoing upper abdominal surgery. | 2007 | 17218378 |
| biochemical severity of thyroid ectopia in congenital hypothyroidism demonstrates sexual dimorphism. | a recent study suggested that sexual dimorphism affects initial thyroid function in congenital hypothyroidism (ch) but differs according to aetiology of ch. aims: to determine if sexual dimorphism was associated with biochemical severity of ch and its aetiology in our large british population. | 2007 | 17218725 |
| newborn screening for congenital hypothyroidism. | most neonates born with congenital hypothyroidism (ch) have normal appearance and no detectable physical signs. hypothyroidism in the newborn period is almost always overlooked and delayed diagnosis leads to the most severe outcome of ch, mental retardation, emphasizing the importance of neonatal screening. blood spot t4 or tsh or both can be used in neonatal screening for ch. the latter, which is more sensitive, is not cost effective, so the first two are used in different programs in the world ... | 2006 | 17220056 |