| impact of previous surgery on endoscopic extraperitoneal radical prostatectomy. | to assess the impact of previous surgery on endoscopic extraperitoneal radical prostatectomy (eerpe). | 2005 | 15708047 |
| non-specific tolerance induced by staphylococcal enterotoxin b in treating high risk corneal transplantation in rats. | to explore the role of staphylococcal enterotoxin b (seb) in treating high risk corneal keratoplasty in rats. | 2005 | 15722320 |
| hematologic and biochemical abnormalities indicating iron deficiency are associated with decreased reticulocyte hemoglobin content (chr) and reticulocyte volume (rmcv) in dogs. | the advia 120 automated hematology system uses low- and high-angle light scatter to determine individual rbc and reticulocyte volume and hemoglobin (hgb) concentration. current hematologic and biochemical markers of iron status in the dog are insensitive, and results may be highly variable, especially in the presence of concurrent disease (ie, inflammation, neoplasia). reticulocyte hgb content (chr) has proven useful in detecting early iron deficiency and iron deficiency masked by concurrent dis ... | 2005 | 15732013 |
| concerns in the development of an assay for determination of a highly conjugated adsorption-prone compound in human urine. | concerns in pre-analytical handling of urine samples are discussed using a new kdr kinase inhibitor, 3-[5-(4-methanesulfonyl-piperazin-1-ylmethyl)-1h-indol-2-yl]-1h-quinolin-2-one (compound a), as an example of a case where high light sensitivity and low analyte recovery (high affinity for container surface) were found. the absence of these problems in plasma samples may be a result of the plasma protein content. low recovery of the analyte from urine can be remedied by either changing the conta ... | 2005 | 15734165 |
| histomorphometric comparison of three bioabsorbable gtr barrier membranes in the canine model. | guided tissue regeneration (gtr) using bioabsorbable barrier membranes has shown promising and somewhat predictable clinical results. however, limited information is available on the stages of histologic healing when these types of barriers are used. therefore, the aim of this study was to compare three currently available bioabsorbable gtr barriers (type 1 collagen, polylactide/polyglycolide copolymer, and citric acid copolymer) for the treatment of surgically created periodontal dehiscences in ... | 2005 | 15736779 |
| antibiotic prophylaxis and non-group b streptococcal neonatal sepsis. | to assess the effect of increased use of intravenous penicillin for group b streptococcus (streptococcus agalactiae, gbs) antibiotic prophylaxis on non-gbs neonatal sepsis and antibiotic resistance. | 2005 | 15738028 |
| effects of clomipramine on self-control choice in lewis and fischer 344 rats. | rates of delay discounting (impulsive choice) have been shown to vary among individuals, particularly people who abuse drugs relative to those who do not, but factors that may contribute to these differences have not been identified. to explore a role for possible genetic and neurochemical determinants, lewis (n = 8) and fischer 344 (n = 8) rats were allowed to choose between one food pellet delivered immediately and three food pellets delivered after increasing delays. the delays to the large r ... | 2005 | 15740780 |
| decreased expression of type ii tumor suppressor gene rarres3 in tissues of hepatocellular carcinoma and cholangiocarcinoma. | to analyze the expression of retinoic acid receptor responder 3 (rarres3) protein in paraffin-embedded tissues of hepatocellular carcinoma (hcc) and cholangiocarcinoma (cc), and the correlation of rarres3 production with tumor differentiation. | 2005 | 15742394 |
| factors predicting severe perineal trauma during childbirth: role of forceps delivery routinely combined with mediolateral episiotomy. | anal sphincter injury and its sequelae are a recognized complication of vaginal childbirth. the aim of the present study was to identify risk factors for third- and fourth-degree perineal tears in patients undergoing either spontaneous or vaginal-assisted delivery by forceps routinely combined with mediolateral episiotomy. | 2005 | 15746685 |
| treatment of patients with superficial bladder cancer stratified by risk groups treated with lyophilized moreau-rio de janeiro bcg strain. | the moreau-rio de janeiro bcg strain is considered the most effective to stimulate immunologic activity in mice. the objective of this prospective study was to evaluate bcg results for patients with superficial bladder cancer stratified by risk groups. | 2002 | 15748368 |
| expression of class iii beta tubulin in non-small cell lung cancer is correlated with resistance to taxane chemotherapy. | this study determined the prevalence and the prognostic value of the expression of microtubule components in tumors of 19 patients with non small cell lung cancer receiving taxane-based regimens. patient samples were stained with antibodies directed against total beta tubulin, classes i, ii and iii beta tubulin isotypes, delta2 alpha tubulin, tau protein, and the p-gp protein involved in the classical multidrug resistance phenotype. all tumors were stained with pan-beta tubulin antibody and clas ... | 2005 | 15749640 |
| a comparison of risk factors for postoperative and spontaneous nonarteritic anterior ischemic optic neuropathy. | : whether postoperative non-arteritic ischemic optic neuropathy (naion) is caused by surgery is unsettled. to provide further evidence on this issue, it is useful to compare the characteristics of patients who develop naion following intraocular surgery to those who develop naion spontaneously. | 2005 | 15756128 |
| [efficacy of two helicobacter pylori eradication treatments in children with recurrent abdominal pain]. | to compare clinical and bacteriologic efficacy of two therapeutic trials to eradicate helicobacter pylori (h. pylori) in two series of pediatric patients with recurrent abdominal pain (rap). | 2004 | 15757155 |
| the slow afterhyperpolarization in hippocampal ca1 neurons covaries with spatial learning ability in aged fisher 344 rats. | rodents commonly exhibit age-related impairments in spatial learning tasks, deficits widely thought to reflect cellular or synaptic dysfunction in the hippocampus. using whole-cell recordings, we examined the afterhyperpolarization (ahp) in ca1 pyramidal cells in hippocampal slices from young (4-6 months of age) and aged (24-26 months of age) fisher 344 male rats that had been behaviorally characterized in the morris water maze. the slow ahp (sahp) recorded from learning-impaired aged rats (ai) ... | 2005 | 15758171 |
| inhibition of lung tumorigenesis by tea. | tea and tea constituents have been shown by different investigators to inhibit lung tumorigenesis in different animal model systems. this includes lung tumorigenesis in a/j mice induced by 4-(methylnitrosamino)-1-(3pyridyl)-1-butanone (nnk), n-nitrosodiethylamine, benzo[a]pyrene, n-nitrosomethylurea, or cisplatin. inhibition of lung tumorigenesis has also been demonstrated in c3h mice treated with n-nitrosodiethylamine. in most of these experiments, reduction in tumor number and tumor size has b ... | 2005 | 15765923 |
| effects of aging on microvascular oxygen pressures in rat skeletal muscle. | aging alters skeletal muscle vascular geometry and control such that the dynamics of muscular blood flow (q) and o2 delivery (q(o2)) may be impaired across the rest-exercise transition. if, at the onset of muscle contractions, q dynamics are slowed disproportionately to those of muscle o2 uptake (v(o2), microvascular po2 (po2m) would be reduced and blood-tissue o2 transfer compromised. this investigation determined the effects of aging on po2m (a direct reflection of the q(o2)-to-v(o2) ratio), a ... | 2005 | 15766914 |
| dietary arsenic affects dimethylhydrazine-induced aberrant crypt formation and hepatic global dna methylation and dna methyltransferase activity in rats. | cell culture studies have suggested that arsenic exposure results in decreased s-adenosylmethionine (sam), causing dna hypomethylation. previously, we have shown that hepatic sam is decreased and/or s-adenosylhomocysteine increased in arsenic-deprived rats; these rats tended to have hypomethylated dna. to determine the effect of dietary arsenic on dimethylhydrazine (dmh)-induced aberrant crypt formation in the colon, fisher 344 weanling male rats were fed diets containing 0, 0.5, or 50 microg as ... | 2005 | 15772437 |
| polymorphisms related to estrogen and xenobiotic metabolism in healthy turkish women. | polymorphisms which are inherited alterations in the activity of cytochrome p450 1b1 (cyp1b1), catechol o-methyltransferase (comt), manganese superoxide dismutase (mnsod) hold the potential to define differences in estrogen metabolism and, thereby, possibly explain inter-individual differences in cancer susceptibility associated with estrogen-mediated carcinogenesis. | 2005 | 15777990 |
| pancreatic secretory trypsin inhibitor (spink1) gene mutations in patients with acute pancreatitis. | mutations in the secretory trypsin inhibitor (spink1) gene have been found to be associated with hereditary and chronic pancreatitis. there are no previous reports on spink1 mutations in patients with acute pancreatitis (ap). | 2005 | 15782101 |
| [evaluation of infection by helicobacter pylori in hiv positive patients trough enzyme immunoassay and specific amplification of dna]. | the objective of this work was to assess the effectiveness of detection of specific antibodies anti-helicobacter pylori (h. pylori) by elisa and amplification of specific dna by polimerase chain reaction (pcr) as diagnostic methods of infection of h. pylori in hiv positive patients. twenty two patients with hiv infection were studied, with ages between 26 to 35 years, 17 masculine, 55% with gastrointestinal symptoms, controlled in the unit of immunology, chet. inclusion approaches: older than 18 ... | 2005 | 15782536 |
| lack of association between endometriosis and n-acetyl transferase 1 (nat1) and 2 (nat2) polymorphisms in a japanese population. | we investigated the association between endometriosis and polymorphisms in the n-acetyltransferase 1 (nat1) and n-acetyltransferase 2 (nat2) genes in a japanese population, having previously demonstrated a positive association with nat2 polymorphisms in a uk population. | 2005 | 15784508 |
| inhibition of matrix metalloproteinases during chronic allograft nephropathy in rats. | chronic allograft nephropathy (can) belongs to the major causes of long-term kidney allograft failure. one of the histologic hallmarks of can is interstitial fibrosis, influenced by matrix metalloproteinases (mmps) that are controlling extracellular matrix (ecm) degradation. whether mmps affect the development and progression of can is not clear so far. to analyze the role of mmps in can, we investigated the effects of an early and a late application of bay 12-9566, an inhibitor of mmp-2, -3, an ... | 2005 | 15785371 |
| regulation of trophic factor expression by innervating target regions in intraocular double transplants. | trophic factors have been found to play a significant role both in long-term survival processes and in more rapid and dynamic processes in the brain and spinal cord. however, little is known regarding the regulation of expression of growth factors, and how these proteins interact on a cell-to-cell basis. we have studied protein levels of one growth factor known to affect the noradrenergic innervation of the hippocampal formation, namely brain-derived neurotrophic factor (bdnf). the purpose of th ... | 2005 | 15789659 |
| interaction of exercise and adenosine receptor agonist and antagonist on rat heart antioxidant defense system. | this study investigated the interactive effects of acute exercise and adenosine receptor agonist and antagonist on antioxidant enzyme activities, glutathione and lipid peroxidation in the heart of the rat. male fisher-344 rats were divided into six groups and treated as follows: (1) saline control; (2) acute exercise (100% vo2max); (3) r-phenyl isopropyl adenosine (r-pia) (3.46 micromol/kg, i.p.); (4) theophylline (1.70 micromol/kg, i.p.) plus acute exercise; (5) theophylline plus r-pia; and (6) ... | 2005 | 15792369 |
| concanavalin a inhibits pathophysiological effects of anti-ganglioside gq1b antibodies at the mouse neuromuscular synapse. | anti-gq1b antibodies are present in the miller fisher syndrome (mfs), a monophasic neuropathy characterized by ataxia, areflexia, ophthalmoplegia, and sometimes cranial muscle weakness. we have previously shown, at the mouse neuromuscular junction (nmj) ex vivo, that anti-gq1b antibodies, through complement classic pathway activation, block synaptic transmission in a way that resembles the effect of the pore-forming alpha-latrotoxin (alphaltx). in order to clarify the mechanism of these alphaltx ... | 2005 | 15793847 |
| reduced cyclooxygenase involvement in vascular endothelial function in rat renal transplantation. | cardiovascular disease is a major cause of death following renal transplantation. mechanisms leading to vascular dysfunction outside the transplanted organ involve common risk factors such as hypertension, hypercholesterolemia, proteinuria, but immune-mediated factors may also be involved. we hypothesized that transplantation-associated risk factors are involved in the development of vascular dysfunction following renal transplantation. | 2005 | 15795519 |
| [herpetic encephalitis: case series of 15 patients confirmed by polymerase chain reaction]. | encephalitis by herpes simplex virus (hsv) is an sporadic and the most important cause of encephalitis in the western world. the aim of this study was to describe the main clinical features and response to therapy in a representative series of cases. fifteen cases confirmed by polymerase chain reaction were identified in two university hospitals in santiago. average age was 41 years (range 5-78) being 80% over 30 years old. most cases presented with fever and sensorial involvement (80%) or heada ... | 2005 | 15798868 |
| immunohistochemical expression of p16ink4a and bcl-2 according to hpv type and to the progression of cervical squamous intraepithelial lesions. | inactivation of the cell cycle inhibitor gene p16mts1 seems to be involved in human papillomavirus (hpv)-related carcinogenesis because e6 and e7 oncoproteins may impair p16ink4a and, indirectly, bcl-2 functions. in this study, we analyzed the role of immunohistochemical expression of p16ink4a and bcl-2 in hpv-infected cervical biopsies as prognostic markers of the progression of squamous intraepithelial lesion (sil). sixty-five cervical biopsies were stratified into two subgroups according to t ... | 2005 | 15805425 |
| assessment of regional tumor hypoxia using 18f-fluoromisonidazole and 64cu(ii)-diacetyl-bis(n4-methylthiosemicarbazone) positron emission tomography: comparative study featuring micropet imaging, po2 probe measurement, autoradiography, and fluorescent microscopy in the r3327-at and fadu rat tumor models. | to compare two potential positron emission tomography (pet) tracers of tumor hypoxia in an animal model. | 2005 | 15817355 |
| increased oxygenation of intracranial tumors by efaproxyn (efaproxiral), an allosteric hemoglobin modifier: in vivo epr oximetry study. | to determine quantitatively the changes in oxygenation of intracranial tumors induced by efaproxiral, an allosteric hemoglobin modifier. efaproxiral reduces hemoglobin-oxygen binding affinity, which facilitates oxygen release from hemoglobin into surrounding tissues and potentially increases the po(2) of the tumors. | 2005 | 15817356 |
| polychaete worms--a vector for white spot syndrome virus (wssv). | the present work provides the first evidence of polychaete worms as passive vectors of white spot syndrome virus (wssv) in the transmission of white spot disease to penaeus monodon broodstocks. the study was based on live polychaete worms, marphysa spp., obtained from worm suppliers/worm fishers as well as samples collected from 8 stations on the northern coast of tamilnadu (india). tiger shrimp penaeus monodon broodstock with undeveloped ovaries were experimentally infected with wssv by feeding ... | 2005 | 15819425 |
| human papillomavirus genome detection by in situ hybridization in fine-needle aspirates of metastatic lesions from head and neck squamous cell carcinomas. | patients with head and neck squamous cell carcinoma (hnscc) often present with metastatic disease. the diagnosis of metastatic lesions usually is determined by fine-needle aspiration. human papillomavirus (hpv) is now being considered as a causative agent in a subset of hnscc. the objectives of this study were, first; to search for the presence of hpv dna by in situ hybridization (ish) in metastatic lesions from hnscc using alcohol-fixed, archival, cytopathologic material; second, to characteriz ... | 2005 | 15822131 |
| increased microvascular blood content is an early event in colon carcinogenesis. | increased premalignant epithelial microvascular blood content is a common theme in neoplastic transformation; however, demonstration of this phenomenon in colon carcinogenesis has been stymied by methodological limitations. our group has recently developed a novel optics technology, four dimensional elastic light scattering fingerprinting (4d-elf), which allows examination of the colonic mucosal architecture with unprecedented accuracy. in this study, we utilised 4d-elf to probe the preneoplasti ... | 2005 | 15831911 |
| suppression of ovarian cancer cell tumorigenicity and evasion of cisplatin resistance using a truncated epidermal growth factor receptor in a rat model. | the overexpression of the epidermal growth factor receptor (egfr) is associated with a poor prognosis in ovarian cancer. the dominant-negative egfr (egfr-dnr) is a truncated receptor that lacks the tyrosine kinase domain and is devoid of signaling capability. this study tested the effects of a egfr-dnr approach in ovarian cancer cells. nutu-19, a rat ovarian cancer cell line was rendered resistant to cisplatin. both nutu-19 and resistant cells were infected with a retroviral vector containing th ... | 2005 | 15833856 |
| living donor candidates for right hepatic lobe transplantation: evaluation at ct cholangiography--initial experience. | to retrospectively evaluate computed tomographic (ct) cholangiography in the depiction of second-order biliary tract anatomy in living donor candidates for right hepatic lobe transplantation. | 2005 | 15833987 |
| [immune tolerance induced by staphylococcal enterotoxin b for the treatment of rat high-risk corneal transplantation rejection]. | to determine the mechanism of staphylococcal enterotoxin b (seb) induced immune tolerance in the treatment of high-risk corneal transplantation in the rat. | 2005 | 15840343 |
| high prevalence of herpes simplex virus dna in temporal arteritis biopsy specimens. | giant cell arteritis (gca) affecting the cranial arteries is a disease of unknown cause that causes blindness, stroke, and other morbidity. its sudden onset and segmental distribution are suggestive of diseases that involve viral reactivation, and cranial arteries are known to be innervated by ganglia that harbor herpes simplex virus (hsv). we used a high-sensitivity polymerase chain reaction assay to test for hsv dna in specimens from 39 consecutive temporal artery biopsies performed for suspec ... | 2005 | 15842052 |
| osteoprogenitor viability in cell populations isolated from rat femora is not affected by 24 h storage at 4 degrees c. | this study was initiated to determine whether partially dissected bones of rats could be refrigerated for 24 h in saline without losing viability of progenitor cells, specifically osteoprogenitors. this is directly applicable to studies involving bone tissue requiring overnight shipment, for example, studies involving space flown animals, grafting experiments, or transplantation. we evaluated cell populations isolated from the proximal femur of 6-week-old male fisher 344 rats. explants from the ... | 2005 | 15843011 |
| serum protein profiling of lung cancer patients. | lung cancer is one of the leading causes of cancer deaths worldwide. the poor patients prognosis is largely attributable to the lack of effective early detection methods. based on the concept that proteins and peptides can emanate from tumor to the serum, the present study aims to investigate if serum proteins pattern, assessed by a gradient polyacrylamide gel, is capable to discriminate 66 lung cancer patients from 44 healthy donors. additionally, in a group of 10 patients and 10 healthy donors ... | 2004 | 15844662 |
| iron oxide nanoparticle-labeled rat smooth muscle cells: cardiac mr imaging for cell graft monitoring and quantitation. | to perform a quantitative analysis of anionic maghemite nanoparticle-labeled cells in vitro and determine the effect of labeling on signal intensity at magnetic resonance (mr) imaging. | 2005 | 15845788 |
| proteomic analysis of protein nitration in aging skeletal muscle and identification of nitrotyrosine-containing sequences in vivo by nanoelectrospray ionization tandem mass spectrometry. | the nitration of protein tyrosine residues represents an important post-translational modification during development, oxidative stress, and biological aging. to rationalize any physiological changes with such modifications, the actual protein targets of nitration must be identified by proteomic methods. while several studies have used proteomics to screen for 3-nitrotyrosine-containing proteins in vivo, most of these studies have failed to prove nitration unambiguously through the actual locali ... | 2005 | 15851474 |
| successful combination of local cpg-odn and radiotherapy in malignant glioma. | oligodeoxynucleotides containing cpg motifs (cpg-odn) display broad immunostimulating activity and are currently under clinical trial in various malignancies, including recurrent glioblastomas. combining cpg-odn with another therapy that could induce antigen release might enhance tumor-specific immune response. we investigated whether radiotherapy (rt) could be associated advantageously to intratumoral injections of cpg-odn. fisher rats bearing 9l glioma were treated with various combinations of ... | 2005 | 15856470 |
| effect of human stem cells labeled with ferumoxides-poly-l-lysine on hematologic and biochemical measurements in rats. | to determine whether ferumoxides-poly-l-lysine (pll) complex-labeled mesenchymal stem cells (mscs) or ferumoxides-pll complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. | 2005 | 15858093 |
| effect of brain death and non-heart-beating kidney donation on renal function and injury: an assessment in the isolated perfused rat kidney. | ischemic injury to the renal allograft prior to implantation is considered as the major cause of primary non and never-function (pnf) and delayed graft function (dgf). evidence has been put forward that brain dead and non-heart-beating (nhb) donor organs are of marginal quality compared to living donors. the purpose of this study was to evaluate renal function and injury of brain dead and nhb donor kidneys using the isolated perfused rat kidney. | 2003 | 15859914 |
| altered ca2+ sparks and gating properties of ryanodine receptors in aging cardiomyocytes. | to investigate the cellular mechanisms for altered cardiac function in senescence, we measured ca(2+) transients and ca(2+) sparks in ventricular cardiomyocytes from 6- to 24-month-old fisher 344 (f344) rat hearts. the single channel properties of ryanodine receptors from adult and senescent hearts were also studied. in senescent myocytes, we observed a decreased peak [ca(2+)](i) amplitude and an increased time constant for decay (tau), both of which correlated with a reduced ca(2+) content of t ... | 2005 | 15862349 |
| expression of groups i and ii metabotropic glutamate receptors in the rat brain during aging. | age-dependent changes in the expression of group i and ii metabotropic glutamate (mglu) receptors were studied by in situ hybridization, western blot analysis and immunohistochemistry. male fisher 344 rats of three ages (3, 12 and 25 months) were tested. age-related increases in mglu1 receptor mrna levels were found in several areas (thalamic nuclei, hippocampal ca3) with parallel increases in mglu1a receptor protein expression. however, a slight decrease in mglu1a receptor mrna expression in in ... | 2005 | 15862522 |
| [association between drug, alcohol and tobacco use in adolescents and socio-familiar factors]. | substance abuse is the result of interaction between constitutional, environmental and socio-demographic factors. several studies have been demonstrated that the adolescent substance abuse is a serious and growing problem. the aim of our study is to investigate the association between substance, alcohol and tobacco abuse and socio-familiar factors, to evaluate a substance abuse prevention program which decreases substance use in adolescents. a cross-sectional survey was developed and carried out ... | 2005 | 15869171 |
| the ki-67 labeling index is not a useful predictor for the follow-up of cervical intraepithelial neoplasia 1. | our aim was to determine whether the ki-67 immunostaining pattern, present on diagnosis of cervical intraepithelial neoplasia (cin), predicts the change from low-grade to high-grade cin over a 2-year period after diagnosis. | 2004 | 15874879 |
| toxicogenomic analysis of gene expression changes in rat liver after a 28-day oral benzene exposure. | benzene is an industrial chemical, component of automobile exhaust and cigarette smoke. after hepatic bioactivation benzene induces bone marrow, blood and hepatic toxicity. using a toxicogenomics approach this study analysed the effects of benzene at three dose levels on gene expression in the liver after 28 daily doses. nmr based metabolomics was used to assess benzene exposure by identification of characteristic benzene metabolite profiles in urine. the 28-day oral exposure to 200 and 800 mg/k ... | 2005 | 15878777 |
| antecedent infections in fisher syndrome: a common pathogenesis of molecular mimicry. | to assess the production mechanism of anti-gq1b autoantibody in fisher syndrome (fs). | 2005 | 15883324 |
| prevention of atrophic nonunion development by recombinant human bone morphogenetic protein-7. | severe periosteal and soft tissue disruption at the time of fracture may result in the formation of an atrophic nonunion. we have developed a reproducible atrophic nonunion in an animal model. the purpose of this study was to evaluate whether the immediate application of recombinant human bmp-7 to the fracture site could rescue the healing process in this nonunion model. a total of 56 three month old fisher 344 rats were utilized. a 1.25 mm diameter k-wire was inserted into the femur in a retrog ... | 2004 | 15885485 |
| nandrolone decanoate modulates cell cycle regulation in functionally overloaded rat soleus muscle. | functionally overloading rat soleus muscle by synergist ablation induces a rapid increase in mass. muscle remodeling during the first week of overload is critical for the overload-induced growth. anabolic steroid modulation of this overload-induced remodeling response is not well understood. the purpose of this study was to determine whether pretreatment with nandrolone decanoate, a clinically administered anabolic steroid, alters muscle morphology and gene expression related to muscle growth du ... | 2005 | 15886356 |
| cdna representational difference analysis used in the identification of genes related to the aging process in rat kidney. | aging is a complex physiological process by which the functions of many organ systems deteriorate. growing evidence shows that age-related changes and damage are causally related to oxidative stress and inflammatory responses from reactive species. the aim of this study was to identify differentially expressed genes in old and young kidneys of fisher 344 male rats during the aging process using complementary dna representational difference analysis (cdna rda). cdna rda is a subtractive technique ... | 2005 | 15893360 |
| morpholino oligonucleotide-triggered beta-catenin knockdown compromises normal liver regeneration. | wnt/beta-catenin activation is seen during early liver regeneration (lr) observed as stabilization and translocation to the nucleus followed by an overall decrease. however, beta-catenin continues to be in hepatocyte nucleus and membrane, secondary to its increased gene expression at 6-72 h. | 2005 | 15893845 |
| synergistic effect of low dose cyclosporine a and human interleukin 10 overexpression on acute rejection in rat lung allotransplantation. | electroporation mediated transfer of plasmid dna into peripheral muscle results in high transfection efficiency. the aim of this study was to investigate the effect of gene transfer of human il-10 (hil-10) into the tibialis anterior muscle (mta) in combination with low dose cyclosporine a (csa) on acute rejection of lung allografts in the rat. | 2005 | 15896613 |
| pb2+ exposure alters the lens alpha a-crystallin protein profile in vivo and induces cataract formation in lens organ culture. | epidemiological data supports lead exposure as a risk factor for cataract development. previous studies which demonstrated oxidative imbalances in the lens following in vivo pb(2+) exposure support the idea that lead exposure can alter the lens biochemical homeostasis which may ultimately lead to loss of lens clarity with time. alpha-crystallin, a major lens structural protein and molecular chaperone, undergoes various post-translational modifications upon aging which may contribute to decreased ... | 2005 | 15905016 |
| association of nonreassuring fetal heart rate patterns and subsequent cerebral palsy in pregnancies with intrauterine bacterial infection. | we evaluated an association of nonreassuring fetal heart rate (fhr) patterns and subsequent cerebral palsy (cp) in pregnancies with intrauterine bacterial infection. among 10,030 infants born during 1995 to 2000, 139 were complicated with acute intrauterine bacterial infection in labor. the fhr patterns 2 hours immediately before delivery were interpreted according to the guidelines of the national institute of child health and human development. the correlations between the fhr patterns and umb ... | 2005 | 15906211 |
| [peculiarities of lipid peroxidation indices in ligula intestinalis (cestoda: pseudophyllidea) and its host--abramis brama (l.)]. | a comparative analysis of indices of peroxidation lipids in tissues of ligula inteslinalis plerocercoids and in the intermediate fish host, the bream abramis brama, was performed for the content of common lipids (cl), malonate di-aldehyde (mda), which is a product of lipid peroxidation (pol), and common antioxidate activity (caa). the dependence of indices upon size of parasites is recovered. the long-sized parasites had higher rate of mda and intense caa. the higher rate of mda and low one of c ... | 2005 | 15907025 |
| influence of donor pretreatment with dopamine on allogeneic kidney transplantation after prolonged cold storage in rats. | retrospective transplant database analysis revealed that administration of catecholamines to organ donors reduces acute rejection episodes and improves graft survival after renal transplantation. in the present study, the authors investigated the influence of dopamine donor pretreatment before prolonged cold storage on short- and long-term graft outcome after allogeneic kidney transplantation. | 2005 | 15912102 |
| light-induced fluorescence of the human cervix decreases after prostaglandin application for induction of labor at term. | to define the changes in cervical light-induced fluorescence (lif), an index of cross-linked collagen, after the use of locally applied prostaglandin preparations for labor induction at term and the correlation between lif and bishop-score. | 2005 | 15916844 |
| cross-cultural equivalence of feeding beliefs and practices: the psychometric properties of the child feeding questionnaire among blacks and hispanics. | psychometrically sound measures are considered a necessary condition for valid research. this study used structural equation modeling to examine the cross-cultural equivalence of a widely used measure of parental beliefs and practices regarding child feeding, the child feeding questionnaire [birch l.l., fisher j.o., grimm-thomas k., markey c.n., sawyer r., johnson s.l. confirmatory factor analysis of the child feeding questionnaire: a measure of parental attitudes, beliefs and practices about ch ... | 2005 | 15917048 |
| visfatin: gene expression in isolated adipocytes and sequence analysis in obese wokw rats compared with lean control rats. | recently, fukuhara et al. have shown that the novel visfatin is predominantly released from visceral adipocytes and shares metabolic functions with insulin. the authors suggested that there is a relationship between visfatin and the metabolic syndrome in humans. these findings prompted us to clarify the visfatin gene expression from visceral and subcutaneous adipocytes in wokw rats, as an animal model for polygenically inherited metabolic syndrome, compared to lean dark agouti (da) rats. moreove ... | 2005 | 15922301 |
| the influence of immune system stimulation on encapsulated islet graft survival. | the aim of this study was to determine the influence activating of the recipient immune system on the function of microencapsulated islet xenografts. | 2005 | 15928588 |
| age-related decline in actomyosin function. | to understand the molecular basis of the functional decline in aging muscle, we examined the functional (actomyosin atpase) and chemical (cysteine content) changes in actin and myosin purified from the muscles of young (4- to 12-month-old) and old (27- to 35-month-old) fisher 344 rats. using the soluble, catalytically active myosin fragment, heavy meromyosin (hmm), we determined the maximum rate (v(max)) and actin concentration at half v(max) (k(m)) of the actomyosin atpase, using four combinati ... | 2005 | 15933379 |
| effect of daily administration of pyrantel tartrate in preventing infection in horses experimentally challenged with sarcocystis neurona. | to determine whether daily administration of pyrantel tartrate can prevent infection in horses experimentally challenged with sarcocystis neurona. | 2005 | 15934612 |
| local chemotherapy of f98 rat glioblastoma with paclitaxel and carboplatin embedded in liquid crystalline cubic phases. | implanted drug carrier systems for retarded chemotherapy against gliomas are mainly based upon polymers containing nitrosoureas. the authors have developed an intracavitary carrier system of biodegradable liquid crystalline cubic phases encapsulating carboplatin and paclitaxel and studied it for release kinetics, antitumor activity, and survival prolongation. a total of 61 fisher rats with f98 tumors were divided into six treatment groups at day 12 post-inoculation, receiving either no treatment ... | 2005 | 15937642 |
| aging reduces responsiveness to bso- and heat stress-induced perturbations of glutathione and antioxidant enzymes. | aging alters cellular responses to both heat and oxidative stress. thiol-mediated metabolism of reactive oxygen species (ros) is believed to be important in aging. to begin to determine the role of thiols in aging and heat stress, we depleted liver glutathione (gsh) by administering l-buthionine sulfoximine (bso) in young (6 mo) and old (24 mo) fisher 344 rats before heat stress. animals were given bso (4 mmol/kg ip) or saline (1 ml ip) 2 h before heat stress and subsequently heated to a core te ... | 2005 | 15947071 |
| ultrastructural analysis of the fisher to lewis rat model of chronic allograft nephropathy. | chronic allograft nephropathy (can) is the leading cause of graft loss following kidney transplantation. one factor contributing to can is chronic alloimmune injury. however, the involvement of alloantigen-dependent and -independent factors in can is unclear. the pathomechanism of can has been extensively studied by utilizing the fischer-to-lewis (f344-to-lew) rat model. transplant capillaropathy (circumferential multiplication of the peritubular capillary basement membrane) and transplant glome ... | 2005 | 15948867 |
| peripheral injection of sauvagine prevents repeated colorectal distension-induced visceral pain in female rats. | we investigated the effects of peripheral injection of sauvagine, a crf2>crf1 receptor (corticotropin-releasing factor) agonist compared with crf, on two sets of tonic colorectal distension (crds 30, 40, 50 mmhg, 3-min on/off)-induced visceromotor response (vmr) measured as area under the curve (auc) of abdominal muscle contraction in conscious female rats. sauvagine (10 or 20 microg/kg, s.c.) abolished the 226.7+/-64.3% and 90.4+/-38.1% increase in auc to the 2nd crd compared with the 1st crd ( ... | 2005 | 15949637 |
| new algorithms for luria-delbrück fluctuation analysis. | fluctuation analysis is the most widely used approach in estimating microbial mutation rates. development of methods for point and interval estimation of mutation rates has long been hampered by lack of closed form expressions for the probability mass function of the number of mutants in a parallel culture. this paper uses sequence convolution to derive exact algorithms for computing the score function and observed fisher information, leading to efficient computation of maximum likelihood estima ... | 2005 | 15950991 |
| the immunobiology of guillain-barré syndromes. | this presentation highlights aspects of the immunobiology of the guillain-barré syndromes (gbs), the world's leading cause of acute autoimmune neuromuscular paralysis. understanding the key pathophysiological pathways of gbs and developing rational, specific immunotherapies are essential steps towards improving the clinical outcome of this devastating disorder. much of the research into gbs over the last decade has focused on the forms mediated by anti-ganglioside antibodies, and we have made su ... | 2005 | 15958123 |
| selective downregulation of ubiquitin conjugation cascade mrna occurs in the senescent rat soleus muscle. | aging-related alterations of the ubiquitin proteasome pathway (upp) have been reported in locomotor skeletal muscle. specifically, declines in proteasome activity have been observed in the soleus of senescent animals compared to the soleus of young controls. however, the influence of aging on the mrna levels of key components within the ubiquitin conjugation cascade (ucc) remains unknown. we hypothesized that aged soleus muscle would exhibit downregulated expression of select ucc mrna and decrea ... | 2005 | 15963672 |
| anti-disialosyl antibodies mediate selective neuronal or schwann cell injury at mouse neuromuscular junctions. | the human paralytic neuropathy, miller fisher syndrome (mfs) is associated with autoantibodies specific for disialosyl epitopes on gangliosides gq1b, gt1a, and gd3. since these gangliosides are enriched in synaptic membranes, anti-ganglioside antibodies may target neuromuscular junctions (nmjs), thereby contributing to disease symptoms. we have shown previously that at murine nmjs, anti-disialosyl antibodies induce an alpha-latrotoxin-like effect, electrophysiologically characterized by transien ... | 2005 | 15968629 |
| preservation of heat stress induced myocardial hsp 72 in aged animals following caloric restriction. | the major stress inducible heat shock protein (hsp 72) confers myocardial protection from ischemia. a decreased ability to express hsp 72 during homeostatic disruptions has been suggested as a possible mechanism for the increased susceptibility of aged hearts to ischemic stress. given that caloric restriction (cr) has been reported to reverse or delay age-associated cellular senescence, we examined the effect of cr on the ability of aged hearts to induce and accumulate hsp 72. adult (6 months), ... | 2005 | 15970415 |
| effects of clozapine and 2,5-dimethoxy-4-methylamphetamine [dom] on 5-ht2a receptor expression in discrete brain areas. | activation of 5-ht2a receptors has been shown to be an essential component of the discriminative stimulus effects of indoleamine and phenethylamine hallucinogens. the objective of the present study was to determine the neuroanatomical location of the 5ht2a receptors which may be responsible for the stimulus effects of the phenethylamine hallucinogen [-]2,5-dimethoxy-4-methylamphetamine (dom). it was hypothesized that brain areas containing altered 5-ht2a receptor expression in the context of a s ... | 2005 | 15972234 |
| ctla-4 gene polymorphisms in chinese patients with ulcerative colitis. | ulcerative colitis (uc) is characterized by chronic inflammation of the colon and rectum as a result of an exaggerated t-cell response. cytotoxic t lymphocyte-associated antigen 4 (ctla-4) is a t cell-restricted surface molecule induced with tcr or cd28 activation. there is evidence for genetic involvement of ctla-4 in several autoimmune diseases, with the focus on the possible role of genetic variation of the ctla-4 locus. the aim of this study was to investigate ctla-4 gene polymorphisms in pa ... | 2005 | 15973119 |
| heat shock protein 27 in chronic allograft nephropathy: a local stress response. | heat shock protein (hsp) 27 plays a cytoprotective role through its antioxidant, antiapoptotic, and actin-stabilizing properties during cell stress. the authors hypothesized that hsp27 is involved in chronic allograft nephropathy (can), a chronic state of inflammation and stress. | 2005 | 15973165 |
| the effects of isoflurane on airway smooth muscle crossbridge kinetics in fisher and lewis rats. | our aim was to determine how isoflurane modified crossbridge (cb) number and kinetics in airway smooth muscle (asm) and to compare its effects in fisher and lewis rats, two strains with differences in airway responsiveness. the effects of isoflurane (2 mac) on isotonic and isometric contractility in tracheal asm strips were investigated after methacholine (10(-6) m)-induced contraction. cb mechanics and kinetics were analyzed using the formalism of huxley's equations adapted to asm. after isoflu ... | 2005 | 15976220 |
| [inhibition of abdominal abscess formation in rat: mortality by sepsis]. | intra-abdominal abscesses and adhesions play important roles on isolation of a septic process and on organism protection against bacteremia. on the other hand, such phenomenon difficults the afflux of immune factors and antibiotics to the septic site. the purpose of the present study was to evaluate the effect of abscess inhibition on survival of abdominal sepsis. | 2005 | 15976911 |
| localized gene expression following administration of adeno-associated viral vectors via pancreatic ducts. | gene transfer into pancreatic cells in vivo could be of immense therapeutic benefit in cases of type 1 diabetes (t1d) through the production of molecules capable of interrupting the progression of autoimmunity or promoting regeneration of insulin-secreting beta cells. we adapted a clinically relevant surgical technique (endoscopic retrograde cholangiopancreatography) to deliver raav encoding human alpha1-antitrypsin (approved gene symbol serpina1) to the pancreas of 3-week-old fisher 344 rats an ... | 2005 | 15979413 |
| mrs of oligodendroglial tumors: correlation with histopathology and genetic subtypes. | oligodendroglial neoplasms with combined loss of chromosomes 1p and 19q may have a good prognosis and respond to procarbazine-lomustine (ccnu)-vincristine (pcv) chemotherapy. | 2005 | 15985578 |
| [oral clarithromycin as a treatment option in chronic post-operative endophthalmitis]. | to assess the outcome of medical treatment with oral clarithromycin in patients with chronic post-surgical endophthalmitis. | 2005 | 15986274 |
| investigation of genetic heterogeneity in mycobacterium tuberculosis isolates from tuberculosis patients using dna fingerprinting. | dna fingerprinting of mycobacterium tuberculosis (mtb) based on is6110 has been shown to be a powerful epidemiologic tool. restriction enzyme analysis (rea) is a fingerprinting technique, which is used for differentiation and investigation of genetic diversity among mycobacterial species. | 2005 | 15988095 |
| antibiotic-induced mesenteric adenopathy in an intussusception mouse model: a randomized, controlled trial. | idiopathic intussusception is a leading cause of intestinal obstruction in young children. although the etiology remains obscure, lymphoid hyperplasia is found in a majority of cases. antibiotics, the most frequently prescribed medication class in the pediatric population, have been recently associated with intussusception. the authors sought to determine whether enteral antibiotic exposure influences the development of mesenteric adenopathy, bowel dilation or intussusception in an animal model. | 2005 | 15990628 |
| constructs incorporating intralaminar c2 screws provide rigid stability for atlantoaxial fixation. | an in vitro biomechanical study of c1-c2 posterior fusion techniques using a cadaveric model. | 2005 | 15990665 |
| bone regeneration in cranial defects previously treated with radiation. | bone reconstruction in the head and neck region is frequently performed in the context of previous radiation treatment. thus, the effectiveness of tissue engineering approaches for regenerating bone in radiated defects needs to be determined before considering application to patients. incomplete healing is described when using osteoinductive protein therapy alone for bone defects previously treated with radiation. we hypothesized that a different approach using ex vivo gene therapy can heal thes ... | 2005 | 15995502 |
| electrophysiological evidence by single fibre electromyography of neuromuscular transmission impairment in a case of miller fisher syndrome. | miller fisher syndrome is an autoimmune neuropathy characterised by ataxia, areflexia and ophthalmoplegia, with minimal if any limb weakness, and in the majority of cases by high titres of igg anti-gq1b ganglioside antibodies. in vitro electrophysiological experiments have demonstrated that these antibodies induce a transmission blockade at neuromuscular junction either pre- or post-synaptically. we report the case of a 63-year-old man with mfs that shows blood serum negative for anti-gq1b but p ... | 2005 | 15995829 |
| p2x7 receptor stimulation of membrane internalization in a thyrocyte cell line. | using fluorescent membrane markers, we have previously shown that extracellular atp stimulates both exocytosis and membrane internalization in the fisher rat thyroid cell line frtl. in this study, we examine the actions of atp using whole-cell recording conditions that favor stimulation of membrane internalization. atp stimulation of the p2x(7) receptor activated a reversible, ca(2+)-permeable, cation conductance that slowly increased in size without changes in ion selectivity. atp also induced ... | 2005 | 16007499 |
| pharmacological modulation of cholinergic brain activity and its reflection in special eeg frequency ranges from various brain areas in the freely moving rat (tele-stereo-eeg). | due to the electrochemical nature of the communication structure of the brain an intimate relationship between neurotransmitter activity on one side and field potentials (eeg) on the other side can be suspected. in order to learn more about this relationship pharmacological manipulation of the cholinergic transmitter system by means of agonistic and antagonistic receptor active drugs was used. continuous recording of electrical activity from four selected brain areas (frontal cortex, hippocampus ... | 2005 | 16009538 |
| innate mechanisms for bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats. | bifidobacteria comprise a dominant microbial population group in the human intestinal tract with purported beneficial health effects on the host. in this study, we characterized the molecular mechanisms for the initial interaction of probiotic bifidobacterium lactis strain bb12 with native and intestinal epithelial cell (iec) lines. we showed that b. lactis-monoassociated fisher f344 rats transiently induce phosphorylation/activation of the nf-kappab transcriptionally active subunit rela and the ... | 2005 | 16011513 |
| rapid antidepressant response after nocturnal trh administration in patients with bipolar type i and bipolar type ii major depression. | thyrotropin-releasing hormone (trh) is a tripeptide that produces endocrine and behavioral effects in animals and humans. some studies have shown transient antidepressant activity after morning administration of trh. we hypothesized that nocturnal administration of trh, when the circadian sensitivity of the trh receptor is at its peak, may result in a more robust antidepressant effect. | 2005 | 16012274 |
| association of polymorphic alleles of ctla4 with inflammatory bowel disease in the japanese. | to examine an association between the cytotoxic t-lymphocyte antigen 4 (ctla4) gene that plays a role in downregulation of t-cell activation and inflammatory bowel disease consisting of ulcerative colitis (uc) and crohn's disease (cd) in the japanese. | 2005 | 16015687 |
| evaluation of insulin-like growth factor-1 for prevention of radiation-induced spinal cord damage. | to test whether insulin-like growth factor-1 (igf-1) ameliorates radiation-induced spinal cord myelopathy and to establish the dose-effect relationship for this growth factor which has not been administered in conjunction with spinal cord irradiation to date. | 2005 | 16019423 |
| influence of the stage of pregnancy on neospora caninum distribution, parasite loads and lesions in aborted bovine foetuses. | in the present work we have studied in neospra caninum aborted bovine foetuses the influence of foetal age (first, second and third gestational periods) on parasite distribution by nested pcr, parasite loads by real-time pcr and n. caninum associated lesions. for this purpose, a total of 220 aborted foetuses were analysed and detection of n. caninum infection was accomplished by nested-pcr in brain, heart and liver, detecting the presence of the parasite in 72 (32.7%) bovine foetuses. when the d ... | 2006 | 16023188 |
| chronic toxicity and carcinogenicity of dietary administered ammonium sulfate in f344 rats. | chronic toxicity and carcinogenicity studies of ammonium sulfate, used as a food additive in fermentation, were performed in male and female fisher 344 rats at dietary concentrations of 0%, 0.1%, 0.6% and 3.0% in a 52-week toxicity study and 0%, 1.5% and 3.0% in a 104-week carcinogenicity study. treatment with ammonium sulfate caused significant increase in kidney and/or liver weights in males and females of the 3.0% diet group, but no effects were found on survival rate, body weights, and hemat ... | 2006 | 16024153 |
| p63 expression in hydropic abortion and gestational trophoblastic diseases. | gestational trophoblastic diseases are a group of interrelated diseases of trophoblastic tissue that include partial hydatidiform mole, complete hydatidiform mole, invasive mole, choriocarcinoma, and placental site trophoblastic tumor. p63 is a p53 homologue that, in normal placentas, is expressed in the cytotrophoblast cells. the role of p63 in gestational trophoblastic diseases, however, merits further investigation. immunohistochemistry with the p63 antibody (clone 4a4) was performed in forma ... | 2006 | 16026831 |
| an empirical test of the information, motivation and behavioral skills model of antiretroviral therapy adherence. | nearly perfect adherence to demanding antiretroviral therapy (art) is now recognized as essential for hiv-positive patients to realize its life sustaining benefits. despite the dire consequences of non-adherence, a large number of patients do not follow their art regimen. while many factors influence adherence, the literature is dominated by studies on only one or a small set of them. multivariate, theory-based models of adherence behavior are of great interest. the current study tested one such ... | 2005 | 16036253 |
| hiv/aids in fishing communities: challenges to delivering antiretroviral therapy to vulnerable groups. | fishing communities have been identified as among the highest-risk groups for hiv infection in countries with high overall rates of hiv/aids prevalence. vulnerability to hiv/aids stems from, the time fishers and fish traders spend away from home, their access to cash income, their demographic profile, the ready availability of commercial sex in fishing ports and the sub-cultures of risk taking and hyper-masculinity in fishermen. the subordinate economic and social position of women in many fishi ... | 2005 | 16036255 |
| calcium chelation improves spatial learning and synaptic plasticity in aged rats. | impaired regulation of intracellular calcium is thought to adversely affect synaptic plasticity and cognition in the aged brain. comparing young (2-3 months) and aged (23-26 months) fisher 344 rats, stratum radiatum-evoked ca1 field epsps were smaller and long-term potentiation (ltp) was diminished in aged hippocampal slices. resting calcium, in presynaptic axonal terminals in the ca1 stratum radiatum area, was elevated in aged slices. loading the slice with the calcium chelator, bapta-am, depre ... | 2006 | 16039651 |
| complement inhibition abrogates nerve terminal injury in miller fisher syndrome. | a large body of clinical and experimental data indicate that complement activation is an important mechanism for neuronal and glial injury in guillain-barré syndromes. inhibition of complement activation therefore might be expected to limit the progression of the disease. using in vitro and in vivo models of the guillain-barré syndrome variant, miller fisher syndrome, we have shown previously that anti-gq1b ganglioside antibodies target the presynaptic motor nerve terminal axon and surrounding p ... | 2005 | 16049921 |