| soluble cd23 containing b cell supernatants induce ige from peripheral blood b-lymphocytes and costimulate with interleukin-4 in induction of ige. | the role of soluble fragments of cd23 and their relationship to interleukin-4 (il-4) in the in vitro production of ige by normal human peripheral blood mononuclear cells was examined. most donors' cells were induced to produce ige in vitro by il-4 during a 9- to 21-day culture. this stimulation was not observed in the absence of t cells. inability of il-4 to induce ige in nonresponding cultures was associated with a failure to express cd23 on lev-19+ natural killer cells; cd23 expression on b ce ... | 1990 | 1698844 |
| transfection and gene expression in normal and malignant primary b lymphocytes. | although many different protocols for transfection of lymphoid cell lines exist, successful dna transfer into primary b lymphocytes has, to date, not been demonstrated. we now describe a simple method for gene transfer into highly purified normal and malignant b lymphocytes by electroporation. using a powerful expression vector containing two copies of the cytomegalovirus (cmv) immediate early enhancer linked to the human t cell lymphotropic virus i (htlv i) promoter, we could demonstrate transf ... | 1990 | 1698880 |
| human immunoglobulin vh and vk repertoire revealed by in situ hybridization. | we report in this paper the first analysis of the expression pattern of ig vh and vk families in human adult normal peripheral b lymphocytes, by in situ hybridization using specific vh1 to vh6 and vk1 to vk4 probes, which cover the known human v gene families reported to date. the major families were vh3 and vk1, with the respective gradient vh3 greater than vh4 greater than vh1 greater than vh5 greater than vh6 greater than vh2, and vk1 greater than vk3 greater than vk4 greater than vk2. using ... | 1990 | 1699122 |
| a high-affinity human monoclonal igm antibody reacting with multiple strains of mycoplasma hominis. | human monoclonal antibodies were produced against mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). the immune b lymphocytes were subsequently immortalized by epstein-barr virus transformation followed by somatic cell fusion. using this procedure, several specific anti-m. hominis monoclonal antibodies of mu isotype were obtained exhibiting apparent affinity constants as high as 3 x 10(9)m-1. the p ... | 1990 | 1700092 |
| inhibition of siv/smm replication in vitro by cd8+ cells from siv/smm infected seropositive clinically asymptomatic sooty mangabeys. | several investigators have demonstrated the ability of cd8+ t cells from hiv-1 infected humans and siv infected rhesus macaques to inhibit viral replication in vitro. in this report we show that cd8+ cells from naturally siv infected sooty mangabeys also have the ability to inhibit viral replication in vitro. in addition, initial experiments which seek to elucidate the mechanism and antigen specificity of cd8-mediated suppression are described. | 1990 | 1700128 |
| a monoclonal antibody that neutralizes epstein-barr virus, human cytomegalovirus, human herpesvirus 6, and bacteriophage t4 dna polymerases. | a monoclonal antibody (mab) designated 55h3 was produced by using chemically induced epstein-barr virus genome-positive b95-8 cells. mab 55h3, which reacted with an 85- to 80-kda polypeptide, neutralized epstein-barr virus-encoded dna polymerase activity in crude extracts of chemically induced m-aba, hr-1, and b95-8 cells, as well as the partially purified epstein-barr virus dna polymerase in a dose-dependent manner. the mab also neutralized the virus-encoded dna polymerase activity from cells i ... | 1990 | 1700422 |
| human monoclonal antibody that recognizes the v3 region of human immunodeficiency virus gp120 and neutralizes the human t-lymphotropic virus type iiimn strain. | we describe a human igg1 monoclonal antibody (n701.9b) derived by epstein-barr virus transformation of b cells from a human immunodeficiency virus-seropositive asymptomatic donor. this antibody was shown to recognize the principal neutralizing domain contained within the v3 region of gp120 of the mn strain of human immunodeficiency virus and mn-like strains, as determined by binding to the pb-1 fragment of mn gp120 and to synthetic peptides corresponding to the v3 region of mn and related virus ... | 1990 | 1700435 |
| monoreactive and polyreactive rheumatoid factors produced by in vitro epstein-barr virus-transformed peripheral blood and synovial b lymphocytes from rheumatoid arthritis patients. | the cd5 membrane molecule, initially identified as an exclusive t-cell marker, also defines a phenotypically and functionally distinct b-lymphocyte population. in normal individuals, cd5+ b cells are committed to secrete 'natural' polyreactive (auto)antibodies, that is antibodies, mainly igm, endowed with multiple antigen-binding capabilities, including rheumatoid factor (rf) activity. at variance with this, in rheumatoid arthritis (ra) as well as in other autoimmune conditions, monoreactive aut ... | 1990 | 1700467 |
| virological examinations of patients with aids-related complex/walter-reed 5 enrolled in a double-blind placebo-controlled study with intravenous gammaglobulin administration. prognostic value of anti-p24 determination. the arc-ivig study group. | thirty patients with aids-related complex/walter-reed 5 enrolled in a placebo-controlled double-blind study with high-dose intravenous gammaglobulin administration were tested by quantitating hiv western blot and other serological tests for viral antibodies. furthermore, conventional virus isolation attempts were performed. absence or loss of p24 antibodies during the study period was associated with progression to aids (p = 0.01) and thereby was an earlier prognostic parameter of a poor prognos ... | 1990 | 1700551 |
| ligation of membrane immunoglobulin leads to inactivation of the signal-transducing ability of membrane immunoglobulin, cd19, cd21, and b-cell gp95. | we have examined the ability of membrane immunoglobulin-binding ligands to desensitize several human b-cell surface molecules that normally transduce signals leading to ca2+ mobilization. ligation of membrane igm or igd leads to heterologous desensitization of the reciprocal receptor in epstein-barr virus-transformed b-cell lines and peripheral blood b cells, as evidenced by a failure of cells to mobilize in response to receptor ligation. under these conditions cd19, cd21, and b-cell gp95 ligati ... | 1990 | 1701054 |
| negative staining em for the detection of epstein-barr virus in oral hairy leukoplakia. | the performance of two different em techniques applied for the detection of epstein-barr virus (ebv) in oral hairy leukoplakia (hl) was assessed, i.e. the conventional two-step method of negative staining (cns) and negative staining after airfuge enrichment (ans). scrape specimens from the lateral borders of tongue of 66 hiv-positive patients with or without hl, of 3 patients with infectious mononucleosis and of 10 hiv-negative patients were evaluated. while cns resulted in virus detection only ... | 1990 | 1701196 |
| regressing nude mouse grafts of burkitt's lymphoma x lymphoblastoid cell hybrids show deregulation of the c-myc gene and expression of the ebv latent membrane protein. | somatic cell hybrids between the malignant burkitt lymphoma cell line bl 60 and the non-malignant epstein-barr virus (ebv) immortalized lymphoblastoid cell line (lcl) iarc 277 demonstrate the deregulated c-myc transcription pattern of the parental bl cell line during exponential growth in tissue culture. subcutaneous nude mouse grafts of these hybrids, however, completely regress after an initial growth phase. to investigate whether regression of these grafts is mediated by a down-regulation of ... | 1991 | 1702406 |
| long-term human b cell lines dependent on interleukin-4 and antibody to cd40. | cd40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on b lymphocytes, epithelial cells, and some carcinoma cell lines. human resting b lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic ltk- cell line that had been transfected with the human fc receptor (fc gamma rii/cdw32) and monoclonal antibodies to cd40. in combination with interleukin-4, factor-dependent long-term normal human b cell lines were generated that were consisten ... | 1991 | 1702555 |
| complement alternative pathway activation and control on membranes of human lymphoid b cell lines. | membrane regulatory molecules normally prevent complement activation by autologous cells, therefore we compared the membrane control system of human lymphoid cell lines which activate or not human complement through the alternative pathway (ap). membrane expression of decay-accelerating factor (daf), membrane cofactor protein (mcp), complement receptors (cr)1, cr2 and h was measured either by radioimmunoassay or enzyme-linked immunosorbent assay on cell lysates. soluble extracts of isolated memb ... | 1990 | 1702720 |
| synthetic peptides to identify antigenic determinants on epstein-barr virus gp350/220. | we synthesized three peptides, ma1 - thr19-val28(+tyr) -, ma2 - ser807-ala816-, and ma3-ser718-glu729(+tyr) from the sequence of epstein-barr virus gp350/220 and immunized rabbits with these peptides. rabbit antisera to the peptides had antipeptide radioimmunoassay titers of 1:400 for anti-ma1, 1:200 for anti-ma2, and 1:1600 for anti-ma3. the anti-ma1 serum recognized gp350/220 in western blotting to sds-electrophoresed proteins from 12-o-tetradecanoylphorbol-13-acetate- and n-butyrate-treated b ... | 1990 | 1703134 |
| effect of monoclonal antibodies to epstein-barr virus envelope glycoproteins on epstein-barr virus neutralization and binding to target cell receptors. a comparative analysis. | viral envelope glycoproteins are important in antiviral immunity; however, their precise role in both generating virus-neutralizing antibodies as well as in viral binding to target cell receptors remains largely unexplored. we studied nine monoclonal antibodies (moabs) to epstein-barr virus (ebv) envelope glycoproteins in order to define the role(s) of their respective epitopes in both ebv neutralization and binding to cellular receptors. only four moabs neutralized ebv infectivity, one requirin ... | 1990 | 1703135 |
| efficient and selective presentation of antigen-antibody complexes by rheumatoid factor b cells. | using epstein-barr virus b cell clones and antigen-specific t cell clones, we asked how antigen-antibody complexes are handled by b cells. we found that the only b cells capable of efficient presentation of antigen-antibody complexes are those that bind the complexes via membrane immunoglobulin, i.e., rheumatoid factor-producing b cells and, to a lower extent, antigen-specific b cells. on the contrary, nonspecific b cells, although capable of binding antigen-antibody complexes, fail to present t ... | 1991 | 1703209 |
| effect of fk 506 on epstein-barr virus specific cytotoxic t cells. | | 1991 | 1703259 |
| a general procedure for evaluation of immunological relevance of synthetic peptides: peptides synthesized on paper in enzyme-linked immunosorbent assay. | multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (whatman 3mm paper). paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. the method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of hiv, the epstein-barr virus-determined nucl ... | 1990 | 1703730 |
| assessment of antigenic determinants for the human t cell response against myelin basic protein using overlapping synthetic peptides. | immunization of experimental animals with myelin basic protein (mbp) or with specific mbp encephalitogenic determinants induces an autoimmune central nervous system (cns) disease, experimental allergic encephalomyelitis, often studied as a model for human demyelinating disorders. this study examines the antigenic determinants of mbp recognized by human t cells using overlapping, synthetic peptides and t cell lines and clones isolated from four hla-typed, neurologically normal subjects. t cell li ... | 1991 | 1704014 |
| a human cd5+ b cell clone that secretes an idiotype-specific high affinity igm monoclonal antibody. | we previously demonstrated the occurrence of a naturally arisen human anti-idiotypic b cell clone, that we transformed with ebv (ebv383). we show evidence that ebv383 not only expresses the cd5 surface ag, but also contains the 2.7-kb mrna transcript encoding this protein. in addition, we show the presence of the 3.6-kb mrna precursor. most ig produced by cd5+ b cells are polyreactive natural igm antibodies encoded by unmutated copies of germline vh genes. however, in this study we present data ... | 1991 | 1704396 |
| cytotoxic t lymphocyte discrimination between type a epstein-barr virus transformants is mapped to an immunodominant epitope in ebna 3. | an immunodominant epstein-barr virus (ebv)-specific cytotoxic t lymphocyte (ctl) epitope, represented by peptide 68, has been mapped to the ebv nuclear antigen, ebna 3. the epitope is recognized by class i-restricted ctls through hla-b8 and is functionally active on type a but not type b lymphoblastoid cell lines (lcls). herein we show that peptide 68 is not expressed as a functional ctl epitope by type a lcls infected with an ebv b95-8 isolate. ctls from cultures stimulated with autologous type ... | 1991 | 1704413 |
| identical igm antibodies recognizing a glycine-alanine epitope are induced during acute infection with epstein-barr virus and cytomegalovirus. | we studied antibody production in serial serum samples from patients with acute epstein-barr virus (ebv) and cytomegalovirus (cmv) infections. sera were analyzed both by enzyme-linked immunosorbent assay (elisa) using a synthetic peptide (p62) derived from the glycine-alanine repeating region of the epstein-barr nuclear antigen (ebna-1) and by immunoblotting. in prior studies, we have shown that patients with acute ebv infection make igm antibodies that react with this peptide, that recognize a ... | 1990 | 1704430 |
| t cell-t cell killing is induced by specific epitopes: evidence for an apoptotic mechanism. | epstein-barr virus-specific cytotoxic t lymphocyte clones were shown to be an effective target for their own lysis when incubated in the presence of their specific epitopes but not in the presence of irrelevant epitopes. the mode of cell killing appeared to be by apoptosis and was prevented by previously described inhibitors of the process. degranulation, as measured by serine esterase activity, was involved in this form of t cell-t cell killing. this is the first report of t cell-t cell killing ... | 1991 | 1705281 |
| preservation of specific rna distribution within the chromatin-depleted nuclear substructure demonstrated by in situ hybridization coupled with biochemical fractionation. | biochemical fractionation procedures previously shown to remove 95% of cellular protein, dna, and phospholipid, were combined with fluorescence in situ hybridization to provide a critical evaluation of the retention and spatial preservation of specific primary transcripts within the chromatin-depleted nuclear substructure, operationally defined as the nuclear "matrix." this unique approach made it possible to directly address whether nuclear extraction procedures preserve, create, or destroy rib ... | 1991 | 1705562 |
| activation of epstein-barr virus latent genes protects human b cells from death by apoptosis. | epstein-barr virus (ebv), a human herpesvirus, establishes a persistent asymptomatic infection of the circulating b-lymphocyte pool. the mechanism of virus persistence is not understood but, given the limited lifespan of most b cells in vivo, it seems most likely that ebv-infected cells must gain access to the long-lived memory b-cell pool. here we show in an in vitro system that ebv, through expression of the full set of eight virus-coded 'latent' proteins, can protect human b cells from progra ... | 1991 | 1705663 |
| cd40 stimulation provides an ifn-gamma-independent and il-4-dependent differentiation signal directly to human b cells for ige production. | ige induction from human cells has generally been considered to be t cell dependent and to require at least two signals: il-4 stimulation and t cell/b cell interaction. in the present study we report a human system of t cell-independent ige production from highly purified b cells. when human cells were co-stimulated with a mab directed against cd40 (mab g28-5), there was induction of ige secretion from purified blood and tonsil b cells as well as unfractionated lymphocytes. anti-cd40 alone faile ... | 1991 | 1706382 |
| infection of human thymocytes by epstein-barr virus. | the epstein-barr virus (ebv) causes infectious mononucleosis, and has been strongly associated with certain human cancers. the virus is thought to exclusively bind to b lymphocytes and epithelial cells via receptors (cr2/cd21) that also interact with fragments of the third component of complement (c3). recent evidence, however, has challenged this belief. we have used two-color immunofluorescence analysis using biotin-conjugated ebv and streptavidin-phycoerythrin along with fluorescein-conjugate ... | 1991 | 1706754 |
| inhibition of b cell proliferation with anti-cd19 monoclonal antibodies: anti-cd19 antibodies do not interfere with early signaling events triggered by anti-igm or interleukin 4. | the 95-kda antigen recognized by the anti-cd19 panel of monoclonal antibodies is found on the surface of most cells of the b cell lineage. anti-cd19 antibodies inhibit b cell proliferation in response to anti-ig plus interleukin 4 (il4), but enhance the response to mitogenic concentrations of either phorbol 12-myristate 13-acetate (pma) or epstein-barr virus. this dichotomy in the effect of anti-cd19 antibodies suggested that the inhibitory action may be directed at the transmembrane signaling p ... | 1991 | 1707000 |
| human b cells express membrane-bound and soluble forms of the cd14 myeloid antigen. | the expression of the myeloid differentiation antigen cd14 on the b lineage was analyzed. a cd14-specific monoclonal antibody was used to isolate the antigen from normal b, b-type chronic lymphocytic leukemia cells, and a representative epstein-barr virus-transformed b lymphoblastoid cell line (ebvlcl). a soluble form of this protein was detected in the culture supernatant of all the b cell types tested. the molecule expressed in the normal b and b-type chronic lymphocytic leukemia cells was ide ... | 1991 | 1707133 |
| auto- and polyreactivity of igm from cd5+ and cd5- cord blood b cells. | the presence of the cd5 (67 kda) molecule on the surface of b cells has been considered a marker for cells producing auto- and polyreactive antibodies. cord blood b lymphocytes (rich in cd5+ b cells) have been sorted into cd5 positive and negative populations by flow cytometry using monoclonal antibodies to cd20 and cd5. clones of these populations were obtained by immortalization with epstein-barr virus. clones derived from both cd5+ and cd5- b cells produced igm which was auto- and polyreactiv ... | 1991 | 1707183 |
| expression of the interleukin 6 gene in rheumatoid synovial fibroblasts. | a number of fibroblastoid synovial cell lines have been established from rheumatoid joints. these cell lines were shown to express the interleukin 6 (il-6) gene constitutively, and exposure of these cells to 5 ng/ml of recombinant human interleukin 1 beta (il-1 beta) increased il-6 gene expression. other recombinant human lymphokines, namely interferon-gamma, tumor necrosis factor-alpha, and granulocyte-macrophage colony stimulating factor had no enhancing effect on il-6 gene expression. dexamet ... | 1990 | 1707461 |
| human cytotoxic t lymphocytes against the plasmodium falciparum circumsporozoite protein. | cytotoxic t lymphocytes (ctl) against the circumsporozoite (cs) protein of malaria sporozoites protect against malaria in rodents. although there is interest in developing human vaccines that induce ctl against the plasmodium falciparum cs protein, humans have never been shown to produce ctl against any plasmodium species protein or other parasite protein. we report that when peripheral blood mononuclear cells (pbmc) from three of four volunteers immunized with irradiated p. falciparum sporozoit ... | 1991 | 1707538 |
| degenerate binding of immunogenic peptides to hla-dr proteins on b cell surfaces. | binding of linear fragments of protein antigens to class i or class ii molecules of the mhc is necessary for the stimulation of a cellular immune response. this report describes the binding of a biotinylated t cell determinant from influenza hemagglutinin to class ii proteins on the surface of epstein-barr virus-transformed b lymphocytes. the rapid, simple, and quantitative binding assay involves flow cytometric analysis of transformed b cells stained with fluoresceinated streptavidin following ... | 1990 | 1707655 |
| oligopeptide induction of a secondary cytotoxic t-cell response to epstein-barr virus in vitro. | three epstein-barr virus (ebv) nuclear antigen (ebna)-encoded oligopeptide epitopes have been mapped, each capable of acting as a recognition determinant for class i-restricted lysis by cd8+ cytotoxic t lymphocytes (ctl). this report shows that each peptide, when presented on an appropriate autologous antigen-presenting cell (apc), also stimulates ebv-specific memory t cells present in peripheral blood mononuclear cell (pbmc) populations to develop in vitro into peptide-specific ctl. these ctl s ... | 1991 | 1708166 |
| detection of infectious agents by molecular methods at the cellular level. | the role of molecular methods for the detection of infectious agents at the cellular level continues to grow. for the future pcr and in situ pcr methods appear to hold great potential as adjuncts to current methods, but not without overcoming some obstacles. | 1990 | 1708606 |
| [epstein-barr virus and malignant lymphomas--studies on incidence and localization of viral dna]. | tissue specimens from 198 cases of hodgkin's disease and 151 non-hodgkin lymphomas as well as 34 non-malignant lymph node biopsies were examined for the presence of epstein-barr virus (ebv) dna by polymerase chain reaction. ebv-specific dna sequences were detected in dna extracts of 58% of hodgkin's disease biopsies. non-hodgkin lymphomas and benign lymph node lesions were associated with ebv in far smaller proportions. in hodgkin's disease biopsies, subsequent in situ hybridization revealed an ... | 1990 | 1708622 |
| [demonstration of dna tumor viruses in carcinomas of the upper respiratory tract]. | in situ hybridization (ish) using 35s-labeled probes was applied to the detection of viral dnas in 115 epithelial tumours from various sites. epstein-barr virus (ebv) dna was detected in 83% of undifferentiated nasopharyngeal carcinomas (npc) while none of seven squamous cell npc displayed an ebv-specific autoradiographic signal. also, all other tumours investigated, including thymomas, thymic carcinomas, tonsillar carcinomas and medullary breast carcinomas were negative upon ish to ebv-specific ... | 1990 | 1708623 |
| generation and analysis of clonal igm- and igg-producing human b cell lines expressing an anti-dna-associated idiotype. | this study describes a methodology for generating stable, cloned, ebv-transformed igg- and igm-producing human b cell lines. using these lines we have characterized immunoglobulin v gene utilization in an anti-dna-associated idiotypic system. the 31 anti-dna-associated idiotype is encoded preferentially by the vk1 gene family, and, in all probability, reflects a germ line gene-encoded framework determinant. analysis of these lines indicates that the dna-binding antibodies produced by b cell line ... | 1991 | 1708781 |
| altered hiv expression and ebv-induced transformation in coinfected pbls and pbl subpopulations. | human immunodeficiency virus (hiv) iiib expression and epstein-barr virus (ebv) b95.8-induced transformation were studied during coinfection. coinfection of peripheral blood lymphocyte (pbl) cultures with hiv and ebv resulted in down-regulation of hiv expression. ebv-induced and spontaneous transformation were markedly reduced in pbl cultures exposed to hiv before ebv. on the other hand, transformation was enhanced when pbl cultures were infected with hiv either simultaneous to or after ebv. rec ... | 1991 | 1708929 |
| independent regulation of interleukin 4 (il-4)-induced expression of human b cell surface cd23 and igm: functional evidence for two il-4 receptors. | activation of human b cells with interleukin 4 (il-4) is known to result in increased expression of cd23 (the low-affinity receptor for ige) and sigm. however, whereas cd23 expression is increased by several b cell mitogens, including phorbol 12-myristate 13-acetate, epstein-barr virus, anti-immunoglobulin (ig), and il-4, surface igm (sigm) expression is increased only with il-4, suggesting that expression of each surface antigen is regulated independently. this was confirmed in three different ... | 1991 | 1709049 |
| the tyrosine kinase lck is critically involved in the growth transformation of human b lymphocytes. | epstein-barr virus (ebv) exposure of human b lymphocytes induces rapid, ca(2+)-dependent tyrosine phosphorylation of two cytosolic proteins, one likely the cd21 ebv receptor and another unknown species of 55-60 kda. we now identify the latter protein as the tyrosine kinase lck (p56lck). in t cells many activation events reduce the high constitutive p56lck expression levels typical for that lineage, and they induce the appearance of a 60-kda lck species. we now demonstrate that in b cells exposed ... | 1991 | 1709155 |
| increased proliferation, lytic activity, and purity of human natural killer cells cocultured with mitogen-activated feeder cells. | the addition of mitogen-prestimulated periferal blood lymphocytes (pbl) or epstein-barr virus (ebv)-transformed lymphoblastoid cell lines (lcl) cultures to enriched populations of natural killer (nk) cells obtained from pbl of normal donors in the presence of ril-2 resulted in highly significant increases in proliferation, purity, and cytolytic activity of cultured nk cells. two sources of enriched nk cell preparations were used: (i) adherent-lymphokine activated killer (a-lak) cells obtained by ... | 1991 | 1709827 |
| antibody specificity and immunoglobulin vh gene utilization of human monoclonal cd5+ b cell lines. | human b lymphocytes that bear the cd5 antigen are relatively abundant in early ontogeny and comprise a small fraction of the b cell population in adults. the cd5 b cell subset has attracted much attention because of its possible involvement in autoimmune disease and certain b cell malignancies. to begin to understand the role of cd5 b cells in disease processes, we have generated a panel of ten human monoclonal b cell lines selected for expression of the cd5 antigen. these cell lines were obtain ... | 1991 | 1709863 |
| an epstein-barr virus protein associated with cell growth transformation interacts with a tyrosine kinase. | epstein-barr virus (ebv) encodes two integral membrane proteins in latently infected growth-transformed cells. one of these, lmp1, can transform rodent fibroblasts and induce markers of b-lymphocyte activation. the second, lmp2, colocalizes with lmp1 in a constitutive patch in the ebv-transformed b-lymphocyte plasma membrane. the experiments reported here demonstrate that lmp2 may biochemically interact with lmp1 and that lmp2 closely associates with and is an important substrate for a b-lymphoc ... | 1991 | 1710288 |
| identification of two t-cell epitopes on the candidate epstein-barr virus vaccine glycoprotein gp340 recognized by cd4+ t-cell clones. | current efforts to develop an epstein-barr virus subunit vaccine are based on the major envelope glycoprotein gp340. given the central role of cd4+ t cells in regulating immune responses to subunit vaccine antigens, the present study has begun the work of identifying linear epitopes which are recognized by human cd4+ t cells within the 907-amino-acid sequence of gp340. a panel of gp340-specific cd4+ t-cell clones from an epstein-barr virus-immune donor were first assayed for their proliferative ... | 1991 | 1710291 |
| differential expression of epstein barr viral transcripts for two proteins (tp1 and lmp) in lymphocyte and epithelial cells. | studies presented here show that some functions of the human herpesvirus, ebv, may be transcriptionally differentially expressed in two cell types which carry the same (c15) isolate of this virus. of the 'latent' viral functions investigated, only one (tp2) of the episomally-specific genes that encode terminal proteins (tp1 and tp2) is found to be expressed in the c15 epithelial cell tumour environment, whereas both are transcribed--as different, but related, messengers--in a b-cell line generat ... | 1991 | 1710355 |
| characterization of human monoclonal antibodies directed against the pp65-kd matrix antigen of human cytomegalovirus. | human monoclonal antibodies specific for human cytomegalovirus (cmv) antigens have been established using peripheral blood lymphocytes from a seropositive donor. immortalization of antigen-specific b cells was achieved by epstein-barr virus transformation followed by somatic cell fusion of antigen-specific lymphoblastoid cells. four clones producing high-affinity antibodies (0.2-7 x 10(9) m-1) specific for the viral matrix protein pp65 have been further characterized with respect to epitope spec ... | 1991 | 1710548 |
| allo-cross-reactivity of a human neuraminidase-specific t cell clone dependent on presentation of an endogenous b cell-specific antigen. | t cells specific for foreign antigen recognize a complex of peptides and self-major histocompatibility complex (mhc) molecules and can also cross-react with allo-mhc molecules. it remains controversial, however, what alloreactive t cells exactly recognize. it has been proposed that alloreactive t cells recognize endogenous peptides presented by allo-mhc molecules. to test this hypothesis, we examined an influenza virus-specific t cell clone (6h5), specific for neuraminidase n2 and restricted by ... | 1991 | 1710566 |
| effect of genistein, a tyrosine kinase inhibitor, on latent ebv activation induced by cross-linkage of membrane igg in akata b cells. | the activation of phosphoprotein tyrosine kinases was studied in the regulation of ebv activation in akata cells after cross-linking membrane igg with anti-igg. protein tyrosine phosphorylation was induced in akata cells after stimulation with anti-igg, as determined by immunoblotting with the py20 anti-phosphotyrosine mab. the frequency of phosphotyrosine-activated cells was also measured by immunofluorescence with the py20 antibody. genistein, an inhibitor of tyrosine kinases, at non-cytotoxic ... | 1991 | 1711079 |
| acid depurination after field inversion agarose gel electrophoresis reduces transfer of large dna molecules. | field-inversion gel electrophoresis (fige) is an economical method for the resolution of dna fragments 20 to 1000 kilobase pairs (kbp) in length, sizes beyond the resolving capabilities of normal agarose gel electrophoresis. a theoretical limitation to fige is the subsequent transfer of large dna molecules to membrane supports for hybridization. after normal electrophoresis, uniform and rapid capillary transfer of dna fragments from agarose gels has been previously reported to be facilitated by ... | 1990 | 1711375 |
| selective expression of a vhiv subfamily of immunoglobulin genes in human cd5+ b lymphocytes from cord blood. | human b lymphocytes expressing the cd5 surface antigen (cd5+ b cells) constitute a subset capable of producing polyspecific antibodies recognizing a variety of self antigens. the repertoire of antibodies produced by cd5+ and cd5- b cells is different. however, it is not yet established whether this distribution is reflected in different immunoglobulin variable region gene (igv) use. rearrangement of heavy chain igv (igvh) genes represents one of the first identifiable stages in the maturation of ... | 1991 | 1711557 |
| analysis of epstein-barr virus-binding sites on complement receptor 2 (cr2/cd21) using human-mouse chimeras and peptides. at least two distinct sites are necessary for ligand-receptor interaction. | the predicted amino acid sequence of human complement receptor 2 (cr2, cd21, c3d,g/epstein-barr virus receptor) and its genetic murine homologue are approximately 70% identical. the sequence of each consists of a linear array of 60-70 amino acid repeats designated short consensus repeats (scrs). although they share significant sequence identity, a major difference in the activities of these two proteins has been believed to be the ability of human, but not mouse, cr2 to mediate epstein-barr viru ... | 1991 | 1712014 |
| production of two human hybridomas secreting antibodies to hla-drw11 and--drw8+w12 specificities. | in this study we describe the production of two human monoclonal antibodies (mabs) hmp12 and hmp14, that recognize polymorphic hla-dr specificities. these mabs have been produced by hybridization of antibody-secreting epstein-barr virus-transformed cells with shm-d33 human-mouse heteromyeloma. by microcytotoxicity assay hmp12 mab was found to react with all drw11-positive cells and hmp14 mab with all cells bearing the drw8 or the drw12 specificity. cytotoxic activity of hmp14 was completely remo ... | 1991 | 1712351 |
| demonstration of epstein-barr virus in scrape material of lateral border of tongue in heart transplant patients by negative staining electron microscopy. | scrape material from the lateral border of the tongue of 50 heart transplant patients and 20 controls was studied for the presence of ebv by negative staining electron microscopy. mild oral hairy leukoplakia was observed in two cases. particles of the herpes virus were found in 20% of the specimens. controls were negative for ebv. the study has shown that ebv may be expressed at the lateral border of the tongue during immunosuppression, occasionally resulting in the clinical appearance of hairy ... | 1991 | 1712389 |
| human cells (normal and ataxia telangiectasia) transfected with pr plasmid are hypersensitive to dna strand-breaking agents. | ataxia telangiectasia (at) cells are known to be hypersensitive to ionizing radiations and to drugs such as bleomycin and epipodophyllotoxin vp16, a topoisomerase ii poison. both of these produce dna double-strand breaks even if through different mechanisms. in this work we analyzed the sensitivity to bleomycin and to epipodophyllotoxin of at cells after transfection with pr plasmid. this plasmid, interacting with bacterial sos repair pathways, expresses itself in mammalian cells conferring cell ... | 1991 | 1712424 |
| multiple overlapping homologies between two rheumatoid antigens and immunosuppressive viruses. | amino acid (aa) sequence homologies between viruses and autoimmune nuclear antigens are suggestive of viral involvement in disorders such as systemic lupus erythematosus (sle) and scleroderma. we analyzed the frequency of exact homologies of greater than or equal to 5 aa between 61 viral proteins (19,827 aa), 8 nuclear antigens (3813 aa), and 41 control proteins (11,743 aa). both pentamer and hexamer homologies between control proteins and viruses are unexpectedly abundant, with hexamer matches ... | 1991 | 1712488 |
| polymerase chain reaction (pcr) for detection of mspi polymorphism at the d3s6 locus. | | 1991 | 1712944 |
| evidence of c-myc gene abnormalities in mediastinal large b-cell lymphoma of young adult age. | six cases of mediastinal large b-cell lymphoma (mlcl) with sclerosis were analyzed for the presence and patterns of c-myc and bcl-2 loci rearrangements, and for the presence of epstein-barr virus dna sequences by southern blot hybridization, c-myc gene alterations were found in three of six cases. two cases showed the presence of mutations or small rearrangements at the 3' end of the first exon. the c-myc gene abnormalities found in these two cases are similar to those observed in the translocat ... | 1991 | 1713514 |
| loss of transforming growth factor beta 1 receptors and its effects on the growth of ebv-transformed human b cells. | transforming growth factor-beta (tgf-beta) is a potent negative regulator of normal human b cell growth mediated by exogenous signals, including il-2 and low m.w. b cell growth factor 12 kda (bcgf-12 kda). in the present study, we investigated the regulatory linkage between viral or nonviral transformation of human b cells and the growth inhibitory effects of tgf-beta 1. a panel of ebv+ and ebv- b cell lines, derived either by in vitro ebv b cell transformation, or from cases of lymphoma was use ... | 1991 | 1713611 |
| exclusive expression of epstein-barr virus nuclear antigen 1 in burkitt lymphoma arises from a third promoter, distinct from the promoters used in latently infected lymphocytes. | epstein-barr virus transformation of human b lymphocytes in vitro results in the expression of six viral nuclear antigens (ebnas) and three viral membrane proteins. however, examination of viral gene expression in fresh burkitt lymphoma isolates has revealed expression of only one of the nuclear antigens, ebna-1. previous transcriptional analyses of the ebna-encoding genes demonstrated that all these genes are driven from one of two distal promoters located near the left end of the viral genome, ... | 1991 | 1713681 |
| synthesis of fusion proteins of epstein-barr virus nuclear antigens in e. coli and their antigenicity. | expression and yield in e. coli of a panel of fusion proteins containing various domains of epstein-barr virus nuclear antigens, ebna-1, ebna-2, ebna-3, ebna-4 and ebna-6, were scrutinized. the antigenicity of the ebna fusion proteins against human sera was examined. monospecific antisera to the different ebna domains were produced by immunizing guinea pigs and rabbits. an ebna-6 fusion polypeptide was useful for separating anti-ebna-6 antibody from human sera by immunoaffinity purification. the ... | 1991 | 1713918 |
| autoantigen recognition by thyroid-infiltrating t cells in graves disease. | graves disease is a common form of human autoimmune thyroiditis. it shares many pathological features and hla associations with other, less easily studied, organ-specific autoimmune conditions such as insulin-dependent diabetes mellitus, and hence it is also a useful model for understanding these other diseases. we have previously shown that thyroid-infiltrating t cells in graves disease that have been recently activated in vivo specifically recognize autologous thyroid epithelial cells. however ... | 1991 | 1714602 |
| activation of human b cells through the cd19 surface antigen results in homotypic adhesion by lfa-1-dependent and -independent mechanisms. | addition of cd19 monoclonal antibodies (mab) to highly purified tonsillar b cells resulted in homotypic adhesion and the formation of cell clusters. this response was completely blocked by antibody to lfa-1, indicating an lfa-1-dependent adhesion mechanism. in contrast, aggregate formation by b cells activated with phorbol myristate acetate (pma) was only partially inhibited by anti-lfa-1 antibody, and those formed in response to pma plus cd19 antibody were not inhibited at all, suggesting aggre ... | 1991 | 1715315 |
| a human monoclonal autoantibody specific for human platelet glycoprotein iib (integrin alpha iib) heavy chain. | splenocytes from a patient with chronic, immune-mediated thrombocytopenic purpura (itp) were transformed with epstein-barr virus. a stable lymphoblastoid cell line (lcl) derived from this transformation (2a3) produces igm antibody reactive with platelet glycoprotein iib. 2a3 was fused to the 6-thioguanine-resistant ouabain-resistant, murine-human heteromyeloma cell line, f6. the resultant heterohybridomas were selected by growth in medium containing hypoxanthine/aminopterin/thymidine and ouabain ... | 1990 | 1715776 |
| application of molecular techniques in the rapid diagnosis of ebv-associated oral hairy leukoplakia. | a rapid method for the detection of ebv-dna in paraffin sections of lesions of oral hairy leukoplakia (ohl) is described. the method makes use of advances in molecular technology, including the use of synthetic oligonucleotides with digoxigenin labelling in an in situ hybridisation (ish) reaction, which can be completed in 24 h. using this method, sections from 15 of 17 patients clinically diagnosed as having ohl contained readily detectable ebv-dna in small foci along the upper layers of the st ... | 1991 | 1716319 |
| antibody bnh9 detects red blood cell-related antigens on anaplastic large cell (cd30+) lymphomas. | two new monoclonal antibodies--bnh9 and bnf13--were generated by using a human lung adenocarcinoma cell line and standard hybridoma techniques. both were found to react with epithelial and endothelial cells in routinely fixed and embedded tissues. unexpected membrane labelling of some large cell lymphomas while non-reacting with normal lymphoid cells, prompted further characterisation. the antibodies were found to recognise red blood cell-related oligosaccharide antigens. the specificities were ... | 1991 | 1716454 |
| lymphokine secretion and cytotoxic activity of human cd4+ t-cell clones against bordetella pertussis. | human cd4+ t-cell clones specific for pertussis toxin and other bordetella pertussis antigens have been tested for their cytotoxic activity, lymphokine production, and capacity to induce immunoglobulin synthesis. clones specific for the s1 subunit of pertussis toxin were cytotoxic for autologous epstein-barr virus-transformed b cells, which had been pulsed with the native antigen, the recombinant s1 subunit of pertussis toxin, or synthetic peptides derived from the s1 amino acid sequence. the ki ... | 1991 | 1716614 |
| selective depletion of bone marrow t lymphocytes with anti-cd5 monoclonal antibodies: effective prophylaxis for graft-versus-host disease in patients with hematologic malignancies. | seventy-one patients with hematologic malignancies received bone marrow from a histocompatible sibling (n = 48) or a partially matched relative (n = 23) that had been depleted of cd5+ t cells with either an anti-cd5 mooclonal antibody (moab) plus complement (anti-leu1 + c) or an anti-cd5 moab conjugated to ricin a chain (st1 immunotoxin [st1-it]). these patients received intensive chemoradiotherapy consisting of cytosine arabinoside, cyclophosphamide, and fractionated total body irradiation. bot ... | 1991 | 1717080 |
| epitope mapping of human monoclonal antibodies to hla-b27 by using natural and mutated antigenic variants. | the epitopes defined by three human monoclonal antibodies (mabs) (tr3b6, trcg10, trbh12) against hla-b27 have been mapped by flow cytometry. for this purpose we used murine transfected cells expressing at their surface hybrid antigens between hla-b7 and -b27 and, in addition, epstein-barr virus cell lines expressing the six hla-b27 alleles b*2701 to b*2706. the results indicated that the mabs are domain specific. trbh12 recognizes the first external (alpha-1) domain. residues critical for the tr ... | 1991 | 1717416 |
| in vitro synthesis of antibodies to myelin antigens by epstein-barr virus-transformed b lymphocytes from patients with neurologic disorders. | anti-myelin antibodies can be found in sera from patients with neurologic disorders of suspected immune-mediated pathogenesis such as multiple sclerosis and inflammatory polyneuropathies. however, the specificity of these findings is controversial. in the present study, in vitro synthesis of antibodies to myelin components was compared to their presence in sera in diverse neurological disorders. epstein-barr virus was used to activate b lymphocytes for in vitro antibody production. anti-myelin b ... | 1991 | 1717665 |
| production of macrophage colony-stimulating factor by human lymphoblastoid cell lines. | the in vitro production of macrophage colony-stimulating factor (m-csf) was studied in 14 human lymphoblastoid cell lines and their lineages were ascertained by surface phenotype analysis. m-csf gene transcripts were detected in a t-lymphocyte-derived cell line (ccrf-cem) and 3 b-lymphoblastoid cell lines (im-9, ball-1, and ccrf-sb) by northern-blot analysis. the secretion of m-csf protein into the culture supernatant by each cell line was also studied using an enzyme-linked immunosorbent assay ... | 1991 | 1717796 |
| growing human b lymphocytes in the cd40 system. | one approach to generating human monoclonal antibodies relies on the development of culture techniques allowing the long-term growth of most b lymphocytes, a property of the cd40 system. | 1991 | 1717852 |
| fyn tyrosine kinase associated with fc epsilon rii/cd23: possible multiple roles in lymphocyte activation. | expression of low-affinity fc receptor for ige (fc epsilon rii), which is identical to the lymphocyte differentiation antigen cd23, is associated with activation of lymphoid cells. the mechanism of signal transduction through fc epsilon rii/cd23 was dissected by transfection of cdna coding for fc epsilon rii to the yt human natural killer-like cell line, activation of which was easily detected by the induction of interleukin 2 receptor/p55(tac). cross-linking of fc epsilon rii/cd23 with h107 ant ... | 1991 | 1717997 |
| transcription analysis and sequence of the putative murine cytomegalovirus dna polymerase gene. | the conservation of the herpesvirus dna polymerases has allowed cross-hybridization studies to be used for their identification and mapping on the viral genome. with the use of a dna fragment containing the dna polymerase gene of human cytomegalovirus (hcmv) as a hybridization probe, we were able to localize the dna polymerase gene of murine cytomegalovirus (mcmv) to a region within mcmv ecori fragment b which spans the hindiii site separating hindiii fragments d and h. this site is colinear wit ... | 1991 | 1718083 |
| nucleotide sequence and transcriptional mapping of the major capsid protein gene of pseudorabies virus. | the gene encoding the 142-kda major capsid protein (mcp142) of pseudorabies virus (prv) was isolated and sequenced. nucleotide sequence analysis revealed that the mcp142 gene has a single open reading frame of 3993 nucleotides (nt) encoding 1330 amino acids. the 4400-nt major rna from the mcp142 gene was detected in prv-infected cells. the 5' end of the transcript was located 60 nt upstream of the initiation codon. the 3' end of the transcript was located 18 nt downstream of a putative poly(a) s ... | 1991 | 1718089 |
| aetiological, modifying and lethal factors in cleft lip and palate. | prenatal factors influencing cleft lip (cl), cleft lip and palate (clp) and isolated cleft palate (cp) may account for the development of the defect (aetiological factor) for a change in the degree or type of the defect (modifying factor), or for the death of the embryo (lethal factor). each active factor appears to act in all three directions, all be it at different ratios. this is used for analysis of groups of cleft-effected individuals registered at the department of plastic surgery, prague, ... | 1991 | 1718108 |
| antiperinuclear factor, a marker autoantibody for rheumatoid arthritis: colocalisation of the perinuclear factor and profilaggrin. | the antiperinuclear factor, an autoantibody specific for rheumatoid arthritis, was found in 51/63 (81%) patients with rheumatoid arthritis by indirect immunofluorescence on human buccal mucosa cells. the sensitivity of the antiperinuclear factor test was increased by pretreating the buccal mucosa cells with 0.5% triton-x100. the specificity of the test for rheumatoid arthritis as compared with control serum samples was maintained. the localisation of the perinuclear factor in the keratohyalin gr ... | 1991 | 1718228 |
| characterization of two monoclonal antibodies to epstein-barr virus diffuse early antigen which react to two different epitopes and have different biological function. | five monoclonal antibodies (mabs) were identified using immunofluorescence that were specific for the epstein-barr virus (ebv) encoded 52/50 kda early antigen (ea-d) protein complex. evidence to suggest that these mabs react with the same 52/50 kda ea-d protein was obtained by western blotting, immunoprecipitation and elisa. two of the mabs, 90e2 and 214a9, neutralized ebv dna polymerase activity. the 214a9 mab also inhibited the activity of bacteriophage t4 dna polymerase while the 90e2 mab did ... | 1991 | 1719014 |
| epstein-barr virus nuclear antigen 1 linear epitopes that are reactive with immunoglobulin a (iga) or igg in sera from nasopharyngeal carcinoma patients or from healthy donors. | the entire amino acid sequence of the unique region of the ebna 1 protein was synthesized as a set of 41 20-residue peptides with an overlap of 10 amino acids. the peptides were tested in the enzyme-linked immunosorbent assay for reactivity with immunoglobulin a (iga) and igg in sera from 50 patients with nasopharyngeal carcinoma (npc) as compared with 36 serum samples from healthy epstein-barr virus (ebv)-seropositive donors and 5 serum samples from ebv-negative donors. the most immunoreactive ... | 1991 | 1719023 |
| mapping of the epitopes of epstein-barr virus gp350 using monoclonal antibodies and recombinant proteins expressed in escherichia coli defines three antigenic determinants. | the epstein-barr virus (ebv) major surface membrane antigen (ma), gp350/220, induces antibodies that neutralize virus infectivity in vitro. the ma glycoprotein is encoded by nucleotides 1784 to 4504 of the bamhi l fragment of the ebv genome. to define the antigenic epitopes on gp350, sequences encoding portions of the protein were cloned into an escherichia coli expression system and eight recombinant clones were generated, two overlapping clones representing the c terminus and six overlapping c ... | 1991 | 1719128 |
| evidence for defective repair of cyclobutane pyrimidine dimers with normal repair of other dna photoproducts in a transcriptionally active gene transfected into cockayne syndrome cells. | cockayne syndrome (cs) and xeroderma pigmentosum (xp), autosomal recessive diseases with clinical and cellular hypersensitivity to uv radiation, differ in ability to repair uv dna photoproducts in their overall genome: normal repair in cs, defective repair in xp. in order to characterize a dna repair defect in an active gene in cs, we measured the capacity of cells from patients with cs and xp to reactivate 2 major types of uv-induced dna damage, photoreactivatable (i.e., cyclobutane pyrimidine ... | 1991 | 1719400 |
| nonresponsiveness to an immunodominant epstein-barr virus-encoded cytotoxic t-lymphocyte epitope in nuclear antigen 3a: implications for vaccine strategies. | an immunodominant epstein-barr virus (ebv)-encoded cytotoxic t lymphocyte (ctl) epitope has been mapped to the ebv nuclear antigen 3a. the epitope, represented by the peptide sequence awnagflrgraygld (hereafter termed awna), is restricted through the hla-b8 allele and is expressed by type a but not type b-infected transformants. herein, we show that ebv-specific memory ctls from an hla-b8+ healthy virus carrier, js, did not respond in vitro to awna, even though that individual's endogenously inf ... | 1991 | 1719531 |
| regulation of lymphocyte chloride channels. | | 1991 | 1719768 |
| binding and multiple hydrolytic sites in epitopes recognized by catalytic anti-peptide antibodies. | autoantibodies purified from humans catalyse the hydrolysis of the neurotransmitter, vasoactive intestinal peptide (vip). evidence that the hydrolysis of vip is due to antibodies includes: the antibody preparations are free of detectable non-immunoglobulin (non-ig) contamination; the hydrolytic activity is removed by precipitation with anti-human igg antibody; human b lymphoblastoid cells transformed with epstein-barr virus secrete hydrolytic antibodies in culture; the fab fragments of the antib ... | 1991 | 1720373 |
| biochemical studies on red blood cells from a patient with the inab phenotype (decay-accelerating factor deficiency). | a 38-year-old russian woman (kz) has been identified as the fourth proposita with the inab blood group phenotype. like the first two propositi, she has a chronic intestinal disorder and, as shown for the third proposita, her inab phenotype is demonstrably inherited. kz's serum contained anti-ifc, which reacted with a red blood cell (rbc) membrane component with an mr of 70,000, which is decay accelerating factor (daf). her rbcs lacked all cromer-related blood group antigens and daf. her rbcs wer ... | 1991 | 1720702 |
| search for the critical characteristics of phenotypically different b cell lines, burkitt lymphoma cells and lymphoblastoid cell lines, which determine differences in their functional interaction with allogeneic lymphocytes. | burkitt lymphoma (bl) lines can be grouped according to phenotypic characteristics. group i cells exhibit the phenotype of resting b cells and grow as single cells. such lines can be epstein-barr-virus(ebv)-negative or -positive. group ii and group iii cells are always ebv-positive, they express b cell activation markers, grow in aggregates and resemble in varying degrees lymphoblastoid cell lines (lcl). we studied three groups of bl lines for their capacity to interact with allogeneic lymphocyt ... | 1991 | 1722139 |
| expression of epstein-barr virus nuclear antigens in anti-igm-stimulated b cells following recombinant vaccinia infection and their recognition by human cytotoxic t cells. | cytotoxic t lymphocytes (ctl) recognizing epstein-barr virus (ebv) nuclear antigens (ebna) are an important host defence mechanism in restricting the proliferation of ebv-infected b cells. previously, b-type lymphoblastoid cell lines (lcl) infected with vaccinia recombinants encoding for the ebna proteins have been used to identify a-type-specific ctl epitopes. however, to localize the ctl epitopes encoded by both a- and b-type transformants, b-type lcl are an inappropriate host for vaccinia. in ... | 1991 | 1722774 |
| cd8 lymphocytes during epstein barr virus (ebv) infection: a cd29 positive population is expanded in acute infectious mononucleosis. | the expression of phenotypic markers on cd4 and cd8 lymphocytes during the acute and convalescent phases of epstein barr virus (ebv) induced infectious mononucleosis was examined by two colour flow cytometry. activated cd8 cells constitute the major population increased during acute infectious mononucleosis; in this phase we observed a preferential expansion of the cd8 cd29+ compared to the cd8 cd45ra+ cells. serum soluble cd8 levels were also raised during the acute phase and a correlation with ... | 1991 | 1722959 |
| cross reaction of antibodies to a glycine/alanine repeat sequence of epstein-barr virus nuclear antigen-1 with collagen, cytokeratin, and actin. | p62 is a synthetic peptide which corresponds to the glycine/alanine repeat sequence of epstein-barr virus nuclear antigen-1. it is the main epitope recognised by anti-rheumatoid arthritis nuclear antigen antibodies. it was shown previously that anti-p62 antibodies were raised fourfold in patients with rheumatoid arthritis compared with controls. to examine the possibility that this increase was due to cross reactive autoantibodies binding to p62, anti-p62 antibodies from serum samples taken from ... | 1991 | 1722966 |
| human monoclonal thyroglobulin autoantibodies of high affinity. i. production, characterisation and interaction with murine monoclonal thyroglobulin antibodies. | four hybridomas secreting human thyroglobulin (tg) autoantibodies of different igg subclasses and light chain types (igg1 lambda, igg1 kappa, igg2 lambda and igg2 kappa) were obtained by direct fusion of hashimoto thyroid lymphocytes with the mouse myeloma x63-ag.653. the autoantibodies were specific for human tg and the functional affinities were high (only 2.6-3.9 log10 pm tg required to give 50% inhibition of binding in elisa). using thyroid lymphocytes, 4 lines secreting tg autoantibodies we ... | 1991 | 1723002 |
| immune regulation of epstein-barr virus (ebv): ebv nuclear antigen as a target for ebv-specific t cell lysis. | | 1991 | 1724100 |
| inhibition of hla b8-restricted recognition by unrelated peptides: evidence for allosteric inhibition. | a panel of synthetic peptides representing human lymphocyte antigen (hla) b8, other class i and class ii restricted t cell epitopes and two b cell epitopes, were all able to compete with recognition of a hla b8 restricted epitope by a cytotoxic t cell clone. competition was obtained when the competitor peptides were added either before or after the target epitope. the target epitope also had a slow off rate, implicating allosteric inhibition. the presence of non-specific, allosteric binding site ... | 1991 | 1724770 |
| [large cell anaplastic lymphomas in the immunocompromised host]. | large cell anaplastic (lca) lymphomas are a newly defined tumor entity, which has recently been integrated in the updated kiel classification. the occurrence of cd30+ lca lymphoma in the setting of renal transplant patients has so far been reported only once. this report describes two lca lymphomas of b-cell phenotype in renal transplant patients. the clonal evolution and possible etiologic role of epstein-barr virus (ebv) in lca lymphoma was studied. our findings give further evidence that clon ... | 1991 | 1724824 |
| [expression of latent membrane proteins (lmp) of epstein-barr virus in malignant lymphomas]. | the presence of epstein-barr virus (ebv) dna and antigens was assessed by polymerase chain reaction and immunohistology, respectively, in a total of 92 cases of hodgkin's disease, angioimmunoblastic lymphadenopathy, cd30-positive anaplastic large cell (alc) lymphomas, and aids-associated atypical lymphoproliferations (alp). proportions of the ebv dna-positive lesions showed latent membrane protein (lmp) expression; some of the lmp-positive alc lymphomas and alp cases also displayed ebna2 immunos ... | 1991 | 1724825 |
| in vitro production of anti-neutrophilocyte-cytoplasm-antibodies (anca) by epstein-barr virus-transformed b-cell lines in wegener's granulomatosis. | the frequent detection of anti-neutrophilocyte-cytoplasm-antibodies (anca) in patients with wegener's granulomatosis (wg) led to the supposition that this disease might be of autoimmune nature. for some authors assume that epstein-barr virus (ebv) infection of human b-lymphocytes besides polyclonal activation could reveal the cryptic immune status against different autoantigens in patients with autoimmune diseases we investigated ebv-transformed b-lymphocytes from patients with sjögren's syndrom ... | 1991 | 1725964 |
| antibodies to myelin basic protein and measles virus in multiple sclerosis: precursor frequency analysis of the antibody producing b cells. | antibody-producing b lymphocytes were polyclonally activated and transformed, by epstein-barr virus (ebv), into multiple b lymphoblastoid cell lines in a microculture system. the frequencies of b precursor cells producing antibodies to myelin basic protein (mbp) and measles virus were analyzed in peripheral blood of patients with multiple sclerosis (ms) and control subjects. measles virus-specific b cells were detected at a significantly higher frequency in ms patients (n = 10, p less than 0.005 ... | 1991 | 1725965 |
| production of a monoclonal antibody (2b44) reactive on a shared epitope on dendritic reticulum cells, smooth muscle cells of vessels and reed-sternberg cells. | lymph node cells from a patient with hodgkin's disease (hd) were cultured without epstein-barr virus (ebv) or leukine adjuvant. a cell line (719-ab) emerged from the culture after four weeks. the cell line express cd20 (79%), cd 21 (30%), cd30 (63%), cd 35 (61%) antigens and weakly cd25 (19%). using southern blot technique, the existence of specific ebv dna and polyclonal immunoglobulin genes rearrangement were observed in the cell line. in order to obtain a monoclonal antibodies (moab), mice ba ... | 1991 | 1726334 |
| evidence that two naturally occurring human insulin receptor alpha-subunit variants are immunologically distinct. | the igg from a patient (italy 2 [i2]) with hypoglycemia, due to autoantibodies to the insulin receptor, was purified on protein a sepharose into two fractions that were tested in various human tissues and cells. the igg fraction that bound protein a (absorbed igg [igga]) nearly completely inhibited the binding of 125i-labeled insulin to various cells or tissues (placenta, im-9, adipocytes, hep-2-larynx cells, epstein-barr virus lymphocytes) but not greater than 50% of 125i-labeled insulin bindin ... | 1992 | 1727740 |
| modulation of autoimmunity by intravenous immune globulin through interaction with the function of the immune/idiotypic network. | infusion of intravenous immune globulin (ivig) has resulted in clinical improvement and/or a fall in autoantibody titer in a number of autoimmune diseases in which direct or indirect evidence suggests a pathogenic role for autoantibodies. ivig may react with disease-associated autoantibodies through idiotypic interactions as shown by the following lines of evidence: (1) inhibition of autoantibody activity in f(ab')2 fragments of patients' igg by f(ab')2 fragments of ivig; (2) retention of autoan ... | 1992 | 1728990 |