| impacts of acute severe pulmonary regurgitation on right ventricular geometry and contractility assessed by tissue-doppler echocardiography. | little is known of the impact of acute right ventricular (rv) volume overload on rv function. we assessed the impact of acute severe pulmonary regurgitation (pr) on global and regional rv function by applying novel quantitative echocardiographic markers of myocardial performance in an animal model. | 2010 | 19812060 |
| detection of novel sequences related to african swine fever virus in human serum and sewage. | the family asfarviridae contains only a single virus species, african swine fever virus (asfv). asfv is a viral agent with significant economic impact due to its devastating effects on populations of domesticated pigs during outbreaks but has not been reported to infect humans. we report here the discovery of novel viral sequences in human serum and sewage which are clearly related to the asfarvirus family but highly divergent from asfv. detection of these sequences suggests that greater genetic ... | 2009 | 19812170 |
| international ring trial to detect anti-trichinella igg by elisa on pig sera. | to determine the reproducibility and robustness of an elisa to detect anti-trichinella igg in pig sera which was previously validated at the community reference laboratory for parasites (crlp), a ring trial was organized involving european and extra-european reference laboratories for trichinella. the sensitivity and specificity of the assay determined by the crlp validation resulted to be 100% and 98.29%, respectively. the assay was reproducible, moreover, based on the receiver-operator charact ... | 2009 | 19819075 |
| sequential activation of ground pads reduces skin heating during radiofrequency tumor ablation: in vivo porcine results. | skin burns below ground pads during monopolar rf ablation are increasingly prevalent, thereby hindering the development of higher power rf generators capable of creating larger tumor ablation zones in combination with multiple or new applicators. our goal was to evaluate reduction in skin temperatures via additional ground pads in an in vivo porcine model. three ground pads placed on the animal's abdomen were activated either simultaneously or sequentially, where activation timing was adjusted t ... | 2010 | 19822466 |
| review of african swine fever: transmission, spread and control. | african swine fever is one of the most important and serious diseases of domestic pigs. its highly contagious nature and ability to spread over long distances make it one of the most feared diseases, since its devastating effects on pig production have been experienced not only in most of sub-saharan africa but also in western europe, the caribbean, brazil and, most recently, the caucasus. unlike most diseases of livestock, there is no vaccine, and therefore prevention relies entirely upon preve ... | 2009 | 19831264 |
| cervical spinal cord therapeutics delivery: preclinical safety validation of a stabilized microinjection platform. | the current series represents a preclinical safety validation study for direct parenchymal microinjection of cellular grafts into the ventral horn of the porcine cervical spinal cord. | 2009 | 19834381 |
| cd14 inhibition efficiently attenuates early inflammatory and hemostatic responses in escherichia coli sepsis in pigs. | sepsis is a severe infection-induced systemic inflammatory syndrome. inhibition of downstream inflammatory mediators of sepsis, e.g., tnf-alpha, has failed in clinical trials. the aim of this study was to investigate the effects of inhibiting cd14, a key upstream innate immunity molecule, on the early inflammatory and hemostatic responses in a pig model of gram-negative sepsis. the study comprised two arms, whole live escherichia coli bacteria and e. coli lipopolysaccharide (lps) (n=25 and n=9 a ... | 2010 | 19841036 |
| technical development and initial animal experience with a novel, uncovered, self-expanding, highly flexible aortic stent with improved side branch access. | to present a novel, uncovered, self-expanding aortic stent for use as a thoracoabdominal stent-graft extension to improve distal flow in aortic dissections complicated by malperfusion syndrome and to enhance the remodeling process in the abdominal aorta after thoracic endovascular aortic repair. | 2009 | 19842725 |
| using accumulated degree-days to estimate postmortem interval from the dna yield of porcine skeletal muscle. | determining the age of meat may require dna extraction from tissue that may be frozen, chilled or sold over the counter of butchers. until now, there has no systematic study on the ageing of meat. in the human application, the parallel is the determination of the time of death or postmortem interval (pmi). the purpose of this study was to investigate the effect of accumulated degree-days on the dna yield of skeletal muscle and its possible application to estimate pmi. this study focused on the e ... | 2010 | 19851896 |
| cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice. | the purpose of this study was to develop effective strategies for cooling and cryopreservation of immature porcine testis tissue that maintain its developmental potential. testes from 1-wk-old piglets (sus domestica) were subjected to 1 of 12 cooling/cryopreservation protocols: as intact testes, cooling at 4 degrees c for 24, 48, or 72h (experiment 1); as fragments, programmed slow-freezing with dimethyl sulfoxide (dmso), glycerol, or ethylene glycol (experiment 2); or solid-surface vitrificatio ... | 2010 | 19853904 |
| the effect of tissue degradation on detection of infectious virus and viral rna to diagnose classical swine fever virus. | a considerable part of tissue samples that are collected for the monitoring of classical swine fever (csf) from the wild boar population or from domestic pigs are unsuitable for virus detection using the fluorescent antibody test (fat) or virus isolation (vi), due to tissue degradation. reverse transcription polymerase chain reaction (rt-pcr) has a higher sensitivity than the fat and vi, and is supposed to be less sensitive to sample degradation. reliable and quantitative information on how long ... | 2010 | 19854005 |
| a novel combination of taqman rt-pcr and a suspension microarray assay for the detection and species identification of pestiviruses. | the genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. all are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. it affects both domestic pigs and wild boars. outbreaks of this disease in domestic pigs call for the most serious measures of disease control, including a stamping out policy in europe. since all the member ... | 2010 | 19854591 |
| the robust phylogeny of korean wild boar (sus scrofa coreanus) using partial d-loop sequence of mtdna. | in order to elucidate the precise phylogenetic relationships of korean wild boar (sus scrofa coreanus), a partial mtdna d-loop region (1,274 bp, nc_000845 nucleotide positions 16576-1236) was sequenced among 56 korean wild boars. in total, 25 haplotypes were identified and classified into four distinct subgroups (k1 to k4) based on bayesian phylogenetic analysis using markov chain monte carlo methods. an extended analysis, adding 139 wild boars sampled worldwide, confirmed that korean wild boars ... | 2009 | 19855937 |
| present and future arboviral threats. | arthropod-borne viruses (arboviruses) are important causes of human disease nearly worldwide. all arboviruses circulate among wild animals, and many cause disease after spillover transmission to humans and agriculturally important domestic animals that are incidental or dead-end hosts. viruses such as dengue (denv) and chikungunya (chikv) that have lost the requirement for enzootic amplification now produce extensive epidemics in tropical urban centers. many arboviruses recently have increased i ... | 2010 | 19857523 |
| classical swine fever: comparison of oronasal immunisation with cp7e2alf marker and c-strain vaccines in domestic pigs. | effective oronasal vaccination against classical swine fever (csf) is essential to achieve protection in wild boar. however the currently available live csf vaccines, e.g. c-strain, do not allow serological differentiation between infected and vaccinated animals (diva). a modified live marker vaccine candidate (cp7e2alf) has been recently developed (reimann et al., 2004). this communication reports the comparison of cp7e2alf and c-strain virus vaccines during 98 days following oronasal immunisat ... | 2010 | 19857935 |
| remarkable sequence similarity between the dinoflagellate-infecting marine girus and the terrestrial pathogen african swine fever virus. | heterocapsa circularisquama dna virus (hcdnav; previously designated as hcv) is a giant virus (girus) with a approximately 356-kbp double-stranded dna (dsdna) genome. hcdnav lytically infects the bivalve-killing marine dinoflagellate h. circularisquama, and currently represents the sole dna virus isolated from dinoflagellates, one of the most abundant protists in marine ecosystems. its morphological features, genome type, and host range previously suggested that hcdnav might be a member of the f ... | 2009 | 19860921 |
| tick-borne encephalitis from eating goat cheese in a mountain region of austria. | we report transmission of tick-borne encephalitis virus (tbev) in july 2008 through nonpasteurized goat milk to 6 humans and 4 domestic pigs in an alpine pasture 1,500 m above sea level. this outbreak indicates the emergence of ticks and tbev at increasing altitudes in central europe and the efficiency of oral transmission of tbev. | 2009 | 19861072 |
| single-port, single-operator-light endoscopic robot-assisted laparoscopic urology: pilot study in a pig model. | to present our initial operative experience in which single-port-light endoscopic robot-assisted reconstructive and extirpative urological surgery was performed by one surgeon, using a pig model. | 2010 | 19863530 |
| a comparative study of the biological characters and pathogenesis of bacillus x (sternberg), bacillus icteroides (sanarelli), and the hog-cholera bacillus (salmon and smith). | 1. bacillus x (sternberg) belongs to the colon group. 2. bacillus icteroides (sanarelli) is a member of the hog-cholera group. 3. the various channels of infection, the duration of the disease and the gross and microscopical lesions in mice, guinea-pigs and rabbits are the same for bacillus icteroides and the hog-cholera bacillus. 4. the clinical symptoms and the lesions observed in dogs inoculated intravenously with bacillus icteroides, are reproduced in these animals by infection with the hog- ... | 1900 | 19866945 |
| the structure and differentiation of the specific cellular elements of the pars intermedia of the hypophysis of the domestic pig. | the bearing of these results on the herring-cushing theory of pituitary secretion is apparent. for the first time a true secretion antecedent has been demonstrated in the cells of the pars intermedia, an antecedent which appears in the cells at the same period of development at which active pressor effects may be obtained from the gland extracts. the route of export of this material from the gland to its point of utilization, however, is unknown; it may go by way of the blood or, as required by ... | 1922 | 19868654 |
| characterisation of experimental infections of domestic pigs with genotype 2.1 and 3.3 isolates of classical swine fever virus. | the early identification of classical swine fever epizootics is hampered by difficulties in recognising early signs of infection, due to a lack of specific clinical signs. in addition many textbook descriptions of csf are based on observations of disease caused by historic, mainly genotype 1, strains. our objective was to improve our knowledge of the diverse range of signs that different csfv strains can cause by characterising the experimental infection of domestic pigs with both a recent strai ... | 2010 | 19875252 |
| characterization of selected nutrients and bacteria from anaerobic swine manure lagoons on sow, nursery, and finisher farms in the mid-south usa. | swine (sus scrofa domestica) production in the mid-south usa comprises sow, nursery, and finisher farms. a 2007 packing plant closure started a regional shift from finisher to sow and nursery farms. changes in manure stored in lagoons and land-applied as fertilizer were expected but were unknown because nutrient and bacterial levels had not been characterized by farm type. the objectives of this study were to quantify selected nutrients and bacteria, compare levels by farm types, and project imp ... | 2009 | 19875798 |
| new records of calyptrate dipterans (fanniidae, muscidae and sarcophagidae) associated with the decomposition of domestic pigs in brazil. | the calyptrate dipterans are the most important decomposers of human cadavers. knowledge of their species and distribution are of great importance to forensic entomology, especially because of the enormous diversity in brazil. carcasses of domestic pigs (sus scrofa, l) were the experimental models used to attract calyptrates of forensic interest during the winters of 2006 and 2007 and the summers of 2006 and 2008. a total of 24,423 specimens from 44 species were collected (19 muscidae, 2 fanniid ... | 2009 | 19876568 |
| elicitation of broad ctl response against hiv-1 by the dna vaccine encoding artificial multi-component fusion protein multihiv--study in domestic pigs. | broad ctl response against hiv-1 is one factor that helps to control the viral replication. we have constructed a dna vaccine that encodes a large artificial fusion protein (multihiv) and shown it to be immunogenic in mice, swine and macaques. inbred mice revealed ctl response only against two epitopes due to limited mhc class i variability. to assess the quality of the ctl response we addressed this question in domestic swine. number of presented epitopes varied between 7 and 14 among the five ... | 2009 | 19879232 |
| androgens and estradiol-17beta production by porcine uterine cells: in vitro study. | porcine (sus scrofa domestica) uterine slices harvested during both early pregnancy and luteolysis produce steroid hormones. the aim of the present study was to determine (1) which porcine separated uterine cells secrete androgens: androstenedione (a(4)) and testosterone (t), and estradiol-17beta (e(2)) in culture; (2) if the production of a(4), t and e(2) in the uterine cells is regulated by p4 and ot; (3) if uterine tissues expressed cytochrome p450arom gene (cyp19). uteri were collected on da ... | 2010 | 19880166 |
| endoscopic closure of duodenal perforations by using an over-the-scope clip: a randomized, controlled porcine study. | duodenal perforations during diagnostic upper endoscopy are rare; however, when therapeutic techniques are performed, the reported incidence is as great as 2.8%. surgical repair is usually mandated, but it is associated with significant morbidity and mortality. | 2010 | 19883907 |
| comparison of two different forms of bovine-derived hydroxyapatite in sinus augmentation and simultaneous implant placement: an experimental study. | the aim of this study was to compare two different forms of bovine-derived hydroxyapatite (ha) in sinus augmentation and simultaneous implant placement. | 2009 | 19885412 |
| concentrations of lipoproteins in the serum of wild boar and lithuanian pig breeds followed by principal component and cluster analyses to demonstrate the differences among genotypes. | with the aim of evaluating serum cholesterol and triglyceride concentrations in lithuanian wild boar and lithuanian pig breeds, twelve gilts from three genotype groups were used in the experiment. only the concentration of high-density lipoprotein (hdl) was significantly higher in the serum of wild boar than in serum of domestic pig breeds (p < 0.05). factor loadings of the principal components (pcs) revealed that lipoproteins were positively related in pc1 for wild boars but associations betwee ... | 2009 | 19886266 |
| detection of a novel hepatitis e-like virus in faeces of wild rats using a nested broad-spectrum rt-pcr. | hepatitis e is a rare human disease in developed countries. it is caused by hepatitis e virus (hev), which is probably transmitted zoonotically to humans from domestic pigs and wild boars. multiple reports on the detection of hev-specific antibodies in rats have suggested the presence of an hev-related agent; however, infectious virus or a viral genome has not been demonstrated so far. here, a nested broad-spectrum rt-pcr protocol was developed capable of detecting different hev types including ... | 2010 | 19889929 |
| phylogenetic analysis of recent classical swine fever virus (csfv) isolates from assam, india. | classical swine fever (csf), a highly contagious viral disease of pigs, is endemic in india. as there is no information concerning the accurate genetic typing of classical swine fever virus (csfv) isolates in india, 16 csf viruses isolated during 2005-2007 from domestic pigs in different districts of assam were typed in 5' utr (150 nucleotides). to confirm the genetic typing results and to study the genetic variability, selected viruses were also analyzed in e2 (190 nt) and ns5b gene (409 nt) re ... | 2011 | 19896713 |
| interferon-gamma induction correlates with protection by dna vaccine expressing e2 glycoprotein against classical swine fever virus infection in domestic pigs. | classical swine fever (csf) is a highly contagious viral infection affecting domestic and wild pigs. for classical swine fever virus (csfv), immunization with plasmids expressing different versions of glycoprotein e2 has proven an effective way to induce protection. previously, we have also shown that immunization with dna vaccine expressing glycoprotein e2 (dna-e2) induced specific t helper cell responses in the absence of neutralizing antibodies. however, the role of t cell responses in protec ... | 2010 | 19896784 |
| multiple asian pig origins revealed through genomic analyses. | previous mitochondrial dna (mtdna) studies have suggested that european and asian pig populations were derived through multiple domestication events. we investigated whether domestic pig populations were derived from distinct ancestors within their respective regions, using eight domestic breeds (five european and three asian), and also european and asian wild boar populations. genomic analyses utilized 21 microsatellite markers (ms) selected for their distribution across the pig genome in addit ... | 2010 | 19900568 |
| role of wild suids in the epidemiology of african swine fever. | there is presently no vaccine to combat african swine fever (asf), a viral hemorrhagic fever of domestic pigs that causes up to 100% morbidity and mortality in naive, commercial pig populations. in its endemic setting, asf virus cycles between asymptomatic warthogs and soft ticks, with persistence in exotic locations being ascribed to the almost global distribution of susceptible soft tick and suid hosts. an understanding of the role played by diverse hosts in the epidemiology of this multi-host ... | 2009 | 19915917 |
| synthetic atrial natriuretic peptide improves systemic and splanchnic circulation and has a lung-protective effect during endotoxemia in pigs. | pharmacological blockade of the renin-angiotensin system is thought to maintain gut perfusion during circulatory stress and thereby avoid later failure of distant organs. in this controlled experimental study, we investigated the effects of carperitide, a synthetic atrial natriuretic peptide that inhibits the renin-angiotensin system, on the systemic and splanchnic circulation during fluid-resuscitated endotoxemia in pigs. | 2010 | 19933537 |
| incidence, diversity, and molecular epidemiology of sapoviruses in swine across europe. | porcine sapovirus is an enteric calicivirus in domestic pigs that belongs to the family caliciviridae. some porcine sapoviruses are genetically related to human caliciviruses, which has raised public health concerns over animal reservoirs and the potential cross-species transmission of sapoviruses. we report on the incidence, genetic diversity, and molecular epidemiology of sapoviruses detected in domestic pigs in a comprehensive study conducted in six european countries (denmark, finland, hunga ... | 2010 | 19940055 |
| histomorphologic and ultrastructural lesions of the pancreas in a porcine model of intra-abdominal hypertension. | intra-abdominal hypertension (iah) and abdominal compartment syndrome are increasingly observed in patients with severe acute pancreatitis (sap). the aim of this study was to investigate the effects of iah on pancreatic histology and ultrastructure in a porcine model. we examined 16 intubated and anesthetized domestic pigs with a mean body weight of 50.6 (sd, 3.8) kg. using a co2 pneumoperitoneum, the intra-abdominal pressure was increased to 30 mmhg for an investigation period of 6 or 12 h (eac ... | 2010 | 19940813 |
| animals to heal animals: ethnoveterinary practices in semiarid region, northeastern brazil. | animal-based remedies constitute an integral part of traditional medicine and this is true in brazil as well both in rural and urban areas of the country. due to its long history, zootherapy has in fact become an integral part of folk medicine in the country. the use of these natural resources for medical purposes, however, is not restricted to human diseases treatment, being also widely used for the treatment of animal illnesses. ethnoveterinary is a science that involves the popular practical ... | 2009 | 19941663 |
| a comparison of intracardiac and transesophageal echocardiography to detect left atrial appendage thrombus in a swine model. | transesophageal echocardiography (tee) is the gold standard in the evaluation for left atrial appendage (laa) thrombus in patients with atrial fibrillation (af) and is often performed prior to af ablation. we routinely use intracardiac echocardiography (ice) to assist in af ablation; however, standard right atrial views do not provide adequate visualization of the laa. as the incidence of thrombus in this population is relatively low, tee incurs additional risk, cost, and patient discomfort. nov ... | 2010 | 19943097 |
| correlation between clinico-pathological outcome and typing of haemophilus parasuis field strains. | haemophilus parasuis is the etiologic agent of glässer's disease in pigs, which is pathologically characterized by serofibrinous polyserositis and arthritis. h. parasuis include virulent and non-virulent strains and confirmation of virulence in h. parasuis is still dependent on experimental reproduction of the disease. since the variability in virulence is supported by serotyping and genotyping (particularly, multilocus sequence typing [mlst]), we examined the relationship between the classifica ... | 2010 | 19945233 |
| [dynamics of heterozygosity and its correlation with fitness in a population of domestic pigs]. | variation of heterozygosity at 11 loci for blood group systems (erythrocyte antigens), occurred during 14 years in the domestic pig population of kemerovskaya breed is described. the two estimates computed for the population examined were represented by expected population heterozygosity, as a measure resistant to stochastic fluctuations, and individual heterozygosity, as a measure with the features of a quantitative trait. our results showed that relative fitness of genotypic classes, formed by ... | 2009 | 19947552 |
| long-term shedding of hepatitis e virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies. | pigs are presumed reservoirs for hepatitis e virus (hev) transmission to humans. to examine infection kinetics, two litters of domestic pigs (a and b, each containing 10 piglets) infected naturally with hev were studied until pigs were 6 months old. maternal igg and iga antibodies were detected in litter a piglets, but not in litter b ones. all pigs shed hev in feces when they were 30-110 days old, and 17 developed viremia at 40-100 days of age. phylogenetic analysis revealed a highly close sequ ... | 2010 | 19950246 |
| european wild boars and domestic pigs display different polymorphic patterns in the toll-like receptor (tlr) 1, tlr2, and tlr6 genes. | during the last decade, the toll-like receptors (tlrs) have been extensively studied, and their immense importance in innate immunity is now being unveiled. here, we report pronounced differences--probably reflecting the domestication process and differences in selective pressure--between wild boars and domestic pigs regarding single nucleotide polymorphisms (snps) in tlr genes. the open reading frames of tlr1, tlr2, and tlr6 were sequenced in 25 wild boars, representing three populations, and i ... | 2010 | 19953243 |
| mechanical transmission of vesicular stomatitis new jersey virus by simulium vittatum (diptera: simuliidae) to domestic swine (sus scrofa). | biting flies have been suggested as mechanical vectors of vesicular stomatitis new jersey virus (family rhabdoviridae, genus vesiculovirus, vsnjv) in livestock populations during epidemic outbreaks in the western united states. we conducted a proof-of-concept study to determine whether biting flies could mechanically transmit vsnjv to livestock by using a black fly, simulium vittatum zetterstedt (diptera: simuliidae), domestic swine, sus scrofa l., model. black flies mechanically transmitted vsn ... | 2009 | 19960709 |
| high prevalence of anti-trichinella igg in domestic pigs of the son la province, vietnam. | although several outbreaks of trichinella infection among humans have occurred in northwestern vietnam, no information is available on the circulation of trichinella among domestic pigs. the objective of the present study was to estimate the seroprevalence of anti-trichinella igg in free-roaming pigs (sus scrofa) in the son la province of northwestern vietnam, where a human outbreak of trichinellosis occurred in june 2008. serum samples were collected from free-roaming pigs of four communes of t ... | 2010 | 19962244 |
| sequential activation of ground pads reduces skin heating during radiofrequency ablation: initial in vivo porcine results. | radiofrequency (rf) ablation is a common treatment modality for inoperable liver cancer. skin burns below ground pads during rf ablations are increasingly prevalent, hindering the development of higher-power rf generators capable of creating larger ablation zones. | 2009 | 19963820 |
| african swine fever. | african swine fever (asf) is a devastating haemorrhagic fever of pigs that causes up to 100% mortality, for which there is no vaccine. it is caused by a unique dna virus that is maintained in an ancient cycle between warthogs and argasid ticks, making it the only known dna arbovirus. asf has a high potential for transboundary spread, and has twice been transported from africa to other continents--europe and subsequently the caribbean and brazil (1957, 1959) and the caucasus (2007). it is also a ... | 2009 | 19967933 |
| a study to demonstrate freedom from trichinella spp. in domestic pigs in switzerland. | trichinellosis is a food-borne zoonotic disease caused by the nematode trichinella spp. many omnivorous and carnivorous animal species can act as host for this parasite, including domestic pigs. to protect public health, it should be ensured that pork should not contain infective trichinella larvae. surveillance for trichinella spp. can be done using direct (larval detection) and indirect (antibody detection) diagnostic techniques. the aim of this study was to demonstrate the absence of infectio ... | 2010 | 19968841 |
| intestinal amebiasis in the domestic pig. | | 1910 | 19971978 |
| transgastric endoluminal gastrojejunostomy: technical development from bench to animal study (with video). | gastrojejunal anastomosis is commonly performed for palliative management of malignant gastric outlet obstruction and bariatric surgery. natural orifice transluminal endoscopic surgery revolutionized the surgical approach to intra-abdominal surgery. this study explored the possibility of performing gastrojejunostomy (gj) by using a hybrid natural orifice transluminal endoscopic surgery approach. | 2010 | 20004893 |
| detection of hepatitis e virus in samples of animal origin collected in hungary. | hepatitis e virus (hev) is an enterically transmitted human pathogen. hev infections are mainly associated with acute, self-limited, icteric hepatitis with an average mortality rate of 1%. animal reservoirs are considered to play an important role in the maintenance of the virus and in the spread of hev to humans. hev-induced seroconversion was described in several species, however clinical hepatitis in animals has not been observed to date. hev strains from animals are genetically closely relat ... | 2010 | 20005644 |
| impact of contrast material volume on quantitative assessment of reperfused acute myocardial infarction using delayed-enhancement 64-slice ct: experience in a porcine model. | our purpose in this study was to compare the impact of contrast material volume in delayed-enhancement computer tomography (ct) imaging for assessing acute reperfused myocardial infarction. | 2010 | 20017004 |
| tylosin and chlorotetracycline decrease the duration of fecal shedding of e. coli o157:h7 by swine. | previously we have shown that experimentally infected swine, fed an antibiotic-free diet, can become colonized and shed escherichia coli o157:h7 for at least 2 months. however, in epidemiological studies this organism is only rarely recovered from domestic swine and the basis for this discrepancy is not clear. in this report we demonstrate that significantly fewer pigs fed diets containing subtherapeutic levels of either tylosin or chlorotetracycline shed e. coli o157:h7 for longer than 2 weeks ... | 2010 | 20018464 |
| next generation biodegradable ureteral stent in a yucatan pig model. | ureteral stents are commonly used to facilitate kidney drainage but they may produce significant stent symptoms and morbidity, and require a secondary procedure for removal. previous biodegradable stents showed bio-incompatibility or inconsistent degradation, requiring extra procedures to remove undegraded stent fragments. we previously reported a first generation biodegradable stent composed of suture-like material that required placement through the lumen of a sheath and degraded by 10 weeks. ... | 2010 | 20022028 |
| a diet supplemented with l-carnitine improves the sperm quality of piétrain but not of duroc and large white boars when photoperiod and temperature increase. | it has been reported that a diet supplemented with l-carnitine can improve sperm quality in some mammalian species. against this background, the current study seeks to determine the effects of feeding l-carnitine (625 mg day(-1)) on boar semen characteristics (ejaculate volume, sperm concentration, sperm viability, acrosome and mitochondrial sheath integrity, sperm motility, sperm morphology, and osmotic resistance of spermatozoa) in three different porcine breeds (sus domesticus) (piétrain, dur ... | 2010 | 20022096 |
| cranial muscles of the sulawesi babirusa (babyrousa celebensis). | the detailed muscular anatomy of the head of the sulawesi babirusa (babyrousa celebensis) is described for the first time. the results show that the muscular anatomy of the babyrousa is very similar to the pig genus sus, despite long geological separation from it. some differences were noted: the m. parietoauricularis was more clearly separated into two parts in the babirusa than in the domestic pig; the rostral fibres of the m. levator anguli oculi reached the infraorbital sinus in the babirusa ... | 2010 | 20028377 |
| reduced l-type calcium current in ventricular myocytes from pigs with hyperdynamic septic shock. | to hypothesize that reduced l-type calcium current with consequent shortening of cardiac repolarization is present in a clinically relevant porcine model of hyperdynamic septic shock. myocardial depression is a well-recognized manifestation of sepsis and septic shock. reduction of l-type calcium current was demonstrated to contribute to the myocardial depression in endotoxemic rodents. | 2010 | 20029342 |
| porcine torque teno virus: determination of viral genomic loads by genogroup-specific multiplex rt-pcr, detection of frequent multiple infections with genogroups 1 or 2, and establishment of viral full-length sequences. | torque teno virus (ttv) is a non-enveloped virus with a circular, single-stranded dna genome. ttv is currently classified in the unassigned genus anellovirus, and distinct ttvs of tentative species-status infect a wide range of vertebrates. in domestic pigs and wild boars, porcine ttv occurs in two genogroups, ttv1 and ttv2, which are currently detected using only conventional pcr assays. to allow high-throughput testing, the present study describes development of a multiplex real-time (rt)-pcr ... | 2010 | 20031342 |
| protective effect of nac preconditioning against ischemia-reperfusion injury in piglet small bowel transplantation: effects on plasma tnf, il-8, hyaluronic acid, and no. | ischemia-reperfusion (i/r) injury is one of the main factors affecting the function and structure of small bowel transplantation (sbt), by generation of proinflammatory mediators such as reactive oxygen species, reactive nitrogen species, cytokines, and endotoxin. experimental data have demonstrated that n-acetylcysteine (nac) attenuates intestinal i/r injury. the objective of this study was to determine the effect of nac preconditioning on the sbt-i/r induced inflammatory cascade, with particul ... | 2011 | 20036383 |
| defibrillation delivered during the upstroke phase of manual chest compression improves shock success. | the current standard of manual chest compression during cardiopulmonary resuscitation requires pauses for rhythm analysis and shock delivery. however, interruptions of chest compression greatly decrease the likelihood of successful defibrillations, and significantly better outcomes are reported if this interruption is avoided. we therefore undertook a prospective randomized controlled animal study in an electrically induced ventricular fibrillation pig model to assess the effects of timing of de ... | 2010 | 20042857 |
| prevalence of hepatitis e virus-specific antibodies in sera of german domestic pigs estimated by using different assays. | hepatitis e virus is the causative agent of an acute hepatitis in humans. in industrialized countries, autochthonous hepatitis e cases in the past were mainly of undetermined origin, whereupon nowadays some cases may be linked to zoonotic transmission of hev from pigs and wild boars. in contrast to several european countries the hev status of german domestic pigs and a possible risk of transmission are unknown so far. here, a novel peptide-based elisa was used to detect hev-specific antibodies i ... | 2010 | 20045268 |
| development of an ultra-high-pressure liquid chromatography-tandem mass spectrometry multi-residue sulfonamide method and its application to water, manure slurry, and soils from swine rearing facilities. | an analytical method was developed using ultra-high-pressure liquid chromatography-triple quadrupole-tandem mass spectrometry (uhplc-tq-ms/ms) to simultaneously analyze 14 sulfonamides (sa) in 6 min. despite the rapidity of the assay the system was properly re-equilibrated in this time. no carryover was observed even after high analyte concentrations. the instrumental detection limit based on signal-to-noise ratio (s/n)>3, was below 1 pg/microl (5 pg on column) for all sas except sulfachloropyri ... | 2010 | 20047751 |
| molecular characterisation of african swine fever viruses from nigeria (2003-2006) recovers multiple virus variants and reaffirms cvr epidemiological utility. | samples collected from wild and domestic suids in nigeria, over a 3-year period (2003-2006), were evaluated for african swine fever (asf) virus genome presence by targeting three discrete genome regions, namely the 478-bp c-terminal p72 gene region advocated for genotype assignment, a 780-bp region spanning the 5'-ends of the pb125r and pb646l (p72) genes and the hypervariable central variable region (cvr) encoded within the 9rl orf (pb602l). asf virus (asfv) presence was confirmed in 23 of the ... | 2010 | 20052526 |
| seasonal effect on sperm messenger rna profile of domestic swine (sus scrofa). | seasonal infertility is a well-known problem in the modern swine (sus scrofa) industry. the molecular mechanisms responsible for thermal effects on spermatogenesis are, however, just beginning to be elucidated. the existence of specific messenger rna (mrna) remnants contained within freshly ejaculated sperm has been identified in several species. investigators have obtained differential rna profiles of infertile men compared with fertile individuals; however, there are limited to the probes, whi ... | 2010 | 20056359 |
| effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid. | the objective was to investigate the effects of oxygen tension and follicle cells (fcs) during in vitro maturation of porcine oocytes in only porcine (sus scrofa domesticus) follicular fluid (pff), using static and non-static (rotating) culture systems, on the nuclear maturation and subsequent in vitro fertilization of the oocytes. in the first experiment, cumulus-oocyte complexes (cocs) were matured for 48 h in pff supplemented with (+) or without (-) fcs (5.2x10(6) cells/ml), using the static ... | 2010 | 20060577 |
| serological, pathological and polymerase chain reaction studies on mycoplasma hyopneumoniae infection in the wild boar. | there are evidences that wild boar and domestic pig populations share the vulnerability to certain pathogens and, in consequence, the risk of pathogen transmission. based on serological evidences, it is known that mycoplasma hyopneumoniae (mhyo) is able to infect the wild boar. however, if this infection causes lesions and disease in this species is still unknown. the objectives of the present study were to assess the seroprevalence to mhyo, as well as the rates of detection of mhyo (by nested p ... | 2010 | 20060659 |
| comparing the demonstration of freedom from trichinella infection of domestic pigs by traditional and risk-based surveillance. | traditionally, the routine artificial digestion test is applied to assess the presence of trichinella larvae in pigs. however, this diagnostic method has a low sensitivity compared to serological tests. the results from artificial digestion tests in switzerland were evaluated over a time period of 15 years to determine by when freedom from infection based on these data could be confirmed. freedom was defined as a 95% probability that the prevalence of infection was below 0.0001%. freedom was dem ... | 2010 | 20067658 |
| preliminary studies into the characterization of chemical markers of decomposition for geoforensics. | in this paper, we report the results of our preliminary studies into chemical characterization of the fluids produced during decomposition in the absence of a soil matrix. pig (sus domestica) carcasses were used to model the human decomposition process in two separate locations, western australia (perth) and canada (oshawa). analysis involved simple dilution and filtration of the decomposition fluids followed by gas chromatography-mass spectrometry. several previously unreported compounds were d ... | 2010 | 20070470 |
| survival and neurological outcomes after nasopharyngeal cooling or peripheral vein cold saline infusion initiated during cardiopulmonary resuscitation in a porcine model of prolonged cardiac arrest. | we have previously demonstrated that nasopharyngeal cooling initiated during cardiopulmonary resuscitation improves the success of resuscitation. in this study, we compared the effects of nasopharyngeal cooling with cold saline infusion initiated during cardiopulmonary resuscitation on resuscitation outcome in a porcine model of prolonged cardiac arrest. we hypothesized that nasopharyngeal cooling initiated during cardiopulmonary resuscitation would yield better resuscitation outcome when compar ... | 2010 | 20081534 |
| tissue distribution of polybrominated diphenyl ethers (pbdes) in captive domestic pigs, sus scrofa, from a village near an electronic waste recycling site in south china. | the dominant part of pbdes residue in pig tissues was bde-47 accounted for 48.2% approximately 66.9%, followed by bde-99 from 15.9% to 24.2%. when the data were on lipid weight basis, the summation operatorpbdes concentrations in tissues of individual pig showed the same order of liver > muscle, intestine > fat. principal component analysis and pbde congener mean concentration ratios of muscle versus liver (m/l), fat versus liver (f/l) and intestine versus liver (i/l) showed the higher accumulat ... | 2010 | 20091151 |
| ex vivo skin infection model. | over the past decade, the emergence of bacterial strains resistant to conventional antibiotics has necessitated the discovery and development of new antimicrobial therapies. this chapter describes a skin infection model that is based on the use of excised skin derived from the domestic pig. the model conditions mimic the environment of human skin and efficiently support the growth of clinically relevant bacterial and fungal species, thus making it useful for evaluating the activity of antimicrob ... | 2010 | 20094875 |
| spatial and temporal expression of spermadhesin genes in reproductive tracts of male and female pigs and ejaculated sperm. | spermadhesins, a novel protein family identified in the reproductive tract of ungulates, have important roles in reproduction. in this study, the expression of pig (sus domesticus) spermadhesion genes in seminal vesicles, prostate, and bulbourethral glands from birth to sexual maturity and the spatial expression in adult male and female genital tracts and ejaculated sperm of meishan pigs were evaluated by reverse transcription-polymerase chain reaction (rt-pcr). in general, all spermadhesin gene ... | 2010 | 20102779 |
| developmental ability of miniature pig embryos cloned with mesenchymal stem cells. | the present study compared the developmental ability of miniature pig embryos cloned with fetal fibroblasts (ffs), bone marrow-derived mesenchymal stem cells (mscs) and differentiated (osteocytes, adipocytes and chondrocytes) mscs. mscs were isolated from an approximately 1-month-old female miniature pig (t-type, pwg micro-pig((r)), pwg genetics korea). mscs were differentiated into osteocytes, adipocytes and chondrocytes under controlled conditions and characterized by cell surface antigen prof ... | 2010 | 20103985 |
| functional and comparative evaluation of flexible monopolar endoscopic scissors. | the continued success of natural orifice translumenal endoscopic procedures requires reliable, accurate tissue dissection and suture cutting. this study aimed to evaluate a flexible endoscopic scissors prototype. | 2010 | 20108151 |
| discriminating the effect of accelerated compression from accelerated decompression during high-impulse cpr in a porcine model of cardiac arrest. | piston based mechanical chest compression devices deliver compressions and decompressions in an accelerated pattern, resulting in superior haemodynamics compared to manual compression in animal studies. the present animal study compares haemodynamics during two different hybrid compression patterns to a standard compression pattern resembling that of modern mechanical chest compression devices. | 2010 | 20122780 |
| heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in korea. | porcine epidemic diarrhea virus (pedv) has plagued the domestic swine industry in korea causing significant economic impacts on pig production nationwide. in the present study, we determined the complete nucleotide sequences of the spike (s) glycoprotein genes of seven korean pedv isolates. the entire s genes of all isolates were found to be nine nucleotides longer in length than other pedv reference strains. this size difference was due to the combined presence of notable 15 bp insertion and 6 ... | 2010 | 20132850 |
| the influence of penetrative trauma on the rate of decomposition. | an understanding of the factors affecting decomposition is important for the accurate estimation of postmortem interval. an experimental study on the influence of penetrating trauma on decomposition rate was carried out using the domestic pig, sus scrofa. the results of this study were: (i) diptera were preferentially attracted to and oviposited at natural orifices. trauma sites were not preferentially selected for oviposition; (ii) no differences between trauma and non-trauma groups were found ... | 2010 | 20141561 |
| refinement of pig retroperfusion technique: global retroperfusion with ligation of the azygos connection preserves hemodynamic function in an acute infarction model in pigs (sus scrofa domestica). | in ischemic hearts, venous retroperfusion is a potential myocardial revascularization strategy. this study aimed to refine the technical and functional aspects of a pig model of acute myocardial infarction and retroperfusion with respect to the azygos connection. global retroperfusion after ligation of the ramus interventricularis paraconalis (equivalent to the left anterior descending artery in humans) was performed in 16 landrace pigs (sus scrofa domestica). coronary sinus perfusion was perfor ... | 2010 | 20158947 |
| nanofiber micro-dispersed oxidized cellulose as a carrier for topical antimicrobials: first experience. | micro-dispersed oxidized cellulose, already used for hemostasis, might be helpful for introduction of an antimicrobial drug. | 2010 | 20163259 |
| [the influence of hypothermia therapy on enzymology and pathology of lung after cardiac arrest]. | to study the effect of hypothermia on pro-inflammatory mediators in serum, the enzymology and pathology of lung tissue. | 2010 | 20170611 |
| a novel double-endoloop technique for natural orifice transluminal endoscopic surgery gastric access site closure. | effective and safe access site closure is critical for clinical application of natural orifice transluminal endoscopic surgery. | 2010 | 20170912 |
| human perception of vocalizations of domestic piglets and modulation by experience with domestic pigs (sus scrofa). | interspecific communication between humans and pets is possible through vocal cues. we studied how humans with differing experience with domestic pigs (sus scrofa) interpret pig vocalizations. forty-eight ethologists studying pigs, 31 pig-caretakers and 54 naive students evaluated the emotional intensity and valence (negative/positive) of recordings from two negative (castration, isolation) and two positive (reunion with the sow, postsuckling) contexts. they also identified the context in which ... | 2010 | 20175599 |
| reduction of odorants in swine manure by carbohydrate and bacterial amendments. | malodors from pig manure storage pits are a problem lacking a cost-efficient solution particularly for small pig (sus domestica) farms. the objective of this study was to reduce emissions of noxious odorants by changing the conditions in the pig manure to favor an altered microbial community. sugars (maltose, lactose, and saccharose), carbohydrate-rich waste (maltose syrup and wheat flour), and bacterial amendments (lactobacillus plantarum and l. amylophilus) were tested for their effect on manu ... | 2010 | 20176840 |
| artificial selection of the melanocortin receptor 1 gene in chinese domestic pigs during domestication. | black coat colour is common in chinese indigenous domestic pigs, but not among their wild ancestors, and it is thus presumed to be a 'domestication trait.' to determine whether artificial interference contributes to morphological diversification, we examined nucleotide variation from 157 chinese domestic pigs and 40 wild boars in the melanocortin receptor 1 (mc1r) gene, which has a key role in the coat pigmentation of sus scrofa. compared with a pseudogene gpip, our results showed that the joint ... | 2010 | 20179735 |
| a trucut biopsy needle for bipolar radiofrequency ablation of needle tract: a proof-of-concept experiment. | to develop a trucut biopsy needle featuring two electrodes that allow for bipolar radiofrequency (rf) coagulation of the puncture tract. | 2010 | 20179938 |
| peritoneal inflammation in pigs is associated with early mitochondrial dysfunction in liver and kidney. | the objective of this study was to investigate early effects of peritoneal inflammation on the mitochondrial function in the vital organs, liver and kidney, and their relation to inflammatory and oxidative stress mediators. the study was performed on 14 domestic pigs. peritoneal inflammation was induced in anesthetized pigs after a midline laparotomy by autologous feces. fluid resuscitation maintained a map above 60 mmhg. animals were sacrificed 12 h later, and tissue samples were obtained to de ... | 2010 | 20180005 |
| interplay between ovine bone marrow stromal cell antigen 2/tetherin and endogenous retroviruses. | endogenous betaretroviruses (enjsrvs) of sheep are expressed abundantly in the female reproductive tract and play a crucial role in conceptus development and placental morphogenesis. interestingly, the colonization of the sheep genome by enjsrvs is likely still ongoing. during early pregnancy, enjsrv expression correlates with the production of tau interferon (ifnt), a type i ifn, by the developing conceptus. ifnt is the pregnancy recognition signal in ruminants and possesses potent antiviral ac ... | 2010 | 20181686 |
| rapid and selective enzymatic debridement of porcine comb burns with bromelain-derived debrase: acute-phase preservation of noninjured tissue and zone of stasis. | deep burns are associated with the formation of an eschar, which delays healing and increases the risk of infection. surgical debridement of the eschar is, at present, the fastest means to achieve an eschar-free bed, but the process can not differentiate between the viable tissue and the eschar and follow the minute irregularities of the interface between the two. we evaluated the efficacy and selectivity of a novel enzymatic bromelain-based debriding agent, debrase gel dressing (debrase), in a ... | 2010 | 20182376 |
| comparison of articular cartilage repair by autologous chondrocytes with and without in vitro cultivation. | autologous chondrocyte implantation usually requires in vitro cell expansion before implantation. we compared the efficacy of cartilage regeneration by in vitro-expanded chondrocytes at high density and freshly harvested chondrocytes at low density. | 2010 | 20187869 |
| seroprevalence and risk factors associated with toxoplasma gondii infection in pig farms from catalonia, north-eastern spain. | seroprevalence and associated risk factors for toxoplasma gondii infection in pigs were analyzed in 1202 sera samples, including sows and pigs of three, seven, 11, 15 and 20 weeks of age, from 23 farms in catalonia, north-eastern spain. antibodies were tested by the modified agglutination test (mat) at titers > or =1:25. antibodies to t. gondii were found in 228 samples (19.0%; 95% ci: 16.8-21.2). the individual prevalence in animals higher than 7 weeks of age was 22.8% (174/762; 95% ci: 16.6-29 ... | 2010 | 20189210 |
| the effect of posterior distraction on vertebral growth in immature pigs: an experimental simulation of growing rod technique. | experimental study. | 2010 | 20195208 |
| blood meal analysis to identify reservoir hosts for amblyomma americanum ticks. | efforts to identify wildlife reservoirs for tick-borne pathogens are frequently limited by poor understanding of tick-host interactions and potentially transient infectivity of hosts under natural conditions. to identify reservoir hosts for lone star tick (amblyomma americanum)-associated pathogens, we used a novel technology. in field-collected ticks, we used pcr to amplify a portion of the 18s rrna gene in remnant blood meal dna. reverse line blot hybridization with host-specific probes was th ... | 2010 | 20202418 |
| tame affairs: domesticated transposase and domestic pigs. | | 2010 | 20203696 |
| spatial distribution and risk factors of brucellosis in iberian wild ungulates. | the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates. | 2010 | 20205703 |
| evaluation of a hemostatic sponge (tachosil) for sealing of the renal collecting system in a porcine laparoscopic partial nephrectomy survival model. | the objective of this study was to evaluate the efficacy of tachosil (nycomed uk, oxford, buckinghamshire, uk), a hemostatic sponge, to seal major collecting system injuries (in addition to providing an adjunct to hemostasis) after partial nephrectomy in a porcine chronic survival model. | 2010 | 20218894 |
| impact of tube current in the quantitative assessment of acute reperfused myocardial infarction with 64-slice delayed-enhancement ct: a porcine model. | this study evaluated the impact of tube current (mas) in delayed-enhancement computed tomography (ct) imaging for assessing acute reperfused myocardial infarction in a porcine model. | 2010 | 20221710 |
| deciphering modifications in swine cardiac troponin i by top-down high-resolution tandem mass spectrometry. | cardiac troponin i (ctni) is an important regulatory protein in cardiac muscle, and its modification represents a key mechanism in the regulation of cardiac muscle contraction and relaxation. ctni is often referred to as the "gold-standard" serum biomarker for diagnosing patients with acute cardiac injury since it is unique to the heart and released into the circulation following necrotic death of cardiac tissue. the swine (sus scrofa) heart model is extremely valuable for cardiovascular researc ... | 2010 | 20223681 |
| ischemia as a possible effect of increased intra-abdominal pressure on central nervous system cytokines, lactate and perfusion pressures. | the aims of our study were to evaluate the impact of increased intra-abdominal pressure (iap) on central nervous system (cns) cytokines (interleukin 6 and tumor necrosis factor), lactate and perfusion pressures, testing the hypothesis that intra-abdominal hypertension (iah) may possibly lead to cns ischemia. | 2010 | 20230612 |
| mammalian limb loading and chondral modeling during ontogeny. | the adaptive growth response of cartilage, or chondral modeling, can result in changes in joint and limb proportions during ontogeny and ultimately contribute to the adult form. despite hamrick's (1999) reevaluation of the mechanisms of chondral modeling, the process of chondral modeling remains poorly studied in animal models. here, we characterize the macro- and microanatomical responses of the femoral growth plate, articular cartilage, and bone in 15 juvenile sus scrofa domestica subjected to ... | 2010 | 20235322 |
| mutations in the ns1 c-terminal tail do not enhance replication or virulence of the 2009 pandemic h1n1 influenza a virus. | the 'classical' swine h1n1 influenza a virus lineage was established after the devastating 1918 human pandemic virus entered domestic pig herds. a descendent of this lineage recently re-emerged in humans as the 2009 pandemic h1n1 virus. adaptation in pigs has led to several changes in the multifunctional viral ns1 protein as compared with the parental 1918 virus, most notably a k217e substitution that abolishes binding to host crk/crkl signalling adapters, and an 11 aa c-terminal truncation. usi ... | 2010 | 20237225 |
| comparison between locking and non-locking plates for fixation of metacarpal fractures in an animal model. | the use of locking plates increases the primary load to failure, thereby reducing the rate of implant-related failure. the good clinical and biomechanical results of locking plates in long bones might be applicable to treatment of metacarpal fractures. the purpose of this study was to determine strength and stiffness of locking plates in a metacarpal fracture model with mono- and bicortical screw fixation in comparison to non-locking plate mono- and bicortical screw fixation, with both types of ... | 2010 | 20299161 |