| influence of lipopolysaccharide chemotype on the interaction between klebsiella pneumoniae and human polymorphonuclear leucocytes. | a series of isogenic mutants lacking either the o1 (o-:k66) or k66 (o1:k-) antigens or both (o-:k-), some of which had additional defects in their lps core polysaccharide was used to examine the interaction between polymorphonuclear leucocytes (pmnls) and k. pneumoniae serotype o1:k66. in the absence of serum complement, only a o-:k- strain with a deep rough lps chemotype elicited a pmnl-dependent chemiluminescent (cl) response. however, following opsonization of the non-capsulated strains by co ... | 1990 | 1698690 |
| [determination of the enterotoxigenicity of conditionally pathogenic microorganisms using the method of suppressing the dehydrogenase activity of the uv mutant staphylococcus aureus 209 p]. | the s. aureus 209p uf mutant dehydrogenase activity suppression method was used for detection of enterotoxin of enterobacteriaceae opportunistic bacteria. comparison of v. cholerae non 01, e coli and k. pneumonia toxigenic and nontoxigenic strains, neutralization test with antitoxic anticholera serum, comparative study of toxigenicities in paw edema test (according to yu. p. vartanyan) have shown that escherichia and klebsiella toxigenic strains can suppress s. aureus 209p uf mutant dehydrogenas ... | 1990 | 1699048 |
| detection of antibodies to helicobacter pylori cell surface antigens. | serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ... | 1990 | 1699265 |
| the use of enzyme immunoassay (eia) and radiobinding assay to investigate the cross-reactivity of klebsiella antigens and hla b27 in ankylosing spondylitis patients and healthy controls. | the binding of rabbit anti-klebsiella antibodies to tissue-typed lymphocytes obtained from 30 ankylosing spondylitis (as) patients and 54 healthy subjects has been measured by an enzyme immunoassay method. hla b27 positive lymphocytes obtained from either as patients (t = 3.60; p less than 0.001) or healthy subjects (t = 3.77; p less than 0.001) were found to bind klebsiella antibodies to a significantly greater extent than non-b27 lymphocytes obtained from healthy controls. absorption studies d ... | 1990 | 1699267 |
| two-step isolation of bacterial ribonucleic acid without using a chaotropic agent or cesium chloride centrifugation. | a simple rna isolation method was developed to purify bacterial rnas from a large number of samples simultaneously in an hour. the method is based on boiling the cells in the presence of triton x-100 and lysozyme, and then preferential rna precipitation with ammonium acetate. there is no cscl centrifugation required. for the nitrogen-fixing bacterium klebsiella pneumoniae, the depression condition can be maintained during the cell-harvesting process. the intact isolated rnas appeared to be free ... | 1990 | 1699454 |
| failure to detect cross-reacting antibodies to hla-b27.5 and klebsiella pneumoniae nitrogenase in sera from patients with ankylosing spondylitis and reiter's syndrome. | | 1990 | 1701922 |
| expression of two structurally distinct d-galactan o antigens in the lipopolysaccharide of klebsiella pneumoniae serotype o1. | the lipopolysaccharide (lps) molecule is an important virulence determinant in klebsiella pneumoniae. studies on the serotype o1 lps were initiated to determine the basis for antigenic heterogeneity previously observed in the o1 side chain polysaccharides and to resolve apparent ambiguities in the reported polysaccharide structure. detailed chemical analysis, involving methylation and 1h- and 13c-nuclear magnetic resonance studies, demonstrated that the o-side chain polysaccharides of serotype o ... | 1991 | 1704883 |
| effects of granulocyte colony-stimulating factor on hematopoietic injury induced by anticancer drugs in mice. | the in vivo effects of the subcutaneous administration of the granulocyte colony-stimulating factor (g-csf) on chemotherapy-induced hematopoietic injury were evaluated in balb/c mice. mice treated with chemotherapeutic drugs were injected once a day for up to 12 days with 2 micrograms of recombinant human g-csf, and following this, bone marrow cellularity, spleen weight, and peripheral blood cell counts were measured 24 h after cessation of the g-csf treatment. treatment of normal mice had minim ... | 1990 | 1705961 |
| cotranscription of the electron transport protein genes nifj and niff in enterobacter agglomerans 333. | a nucleotide sequence showing extensive homology to the niff gene, which codes for a flavodoxin involved in nitrogen fixation in klebsiella pneumoniae, was localized on the plasmid pea3 of enterobacter agglomerans and determined. the analysis of transcriptional fusions, as well as transcript protection assays, indicated a novel nif gene organization, that is, the cotranscription of nifj and niff. | 1991 | 1708766 |
| surface exposure of o1 serotype lipopolysaccharide in klebsiella pneumoniae strains expressing different k antigens. | surface exposure of the o1 serotype lipopolysaccharide in encapsulated klebsiella pneumoniae strains belonging to different serotypes was examined by using the o1 antigen-specific bacteriophages fc3-1 and fc3-2 in conjunction with immunogold electron microscopy and enzyme immunoassays with specific antisera. despite the presence of the capsular polysaccharide, the o1 antigen was exposed at the cell surface in strains producing k2, k7, k8, k12, k19, k21, k22, k34, k35, k42, k45, k55, k57, k62, k6 ... | 1991 | 1709919 |
| serological relationships of the o antigens of klebsiella pneumoniae o5, escherichia coli o8 and a new o serotype of serratia marcescens. | klebsiella pneumoniae o5, escherichia coli o8 and serratia marcescens 3255 were shown to cross-react in both elisa and immunoblotting. the cross-reaction appeared to be due to the o antigen of their lipopolysaccharide (lps). in addition, there was evidence that the reactions of these strains with their homologous antisera were due, in part, to determinants other than o polysaccharide. | 1991 | 1713181 |
| eric sequences: a novel family of repetitive elements in the genomes of escherichia coli, salmonella typhimurium and other enterobacteria. | we describe a family of highly conserved, enterobacterial repetitive intergenic consensus (eric) sequences, 14 of which have been identified in escherichia coli and salmonella typhimurium and a further three in other enterobacterial species (yersinia pseudotuberculosis, klebsiella pneumoniae and vibrio cholerae). eric sequences are 126 bp long and appear to be restricted to transcribed regions of the genome, either in intergenic regions of polycistronic operons or in untranslated regions upstrea ... | 1991 | 1713281 |
| comparative study of the structure/function relationship of wild-type and structurally modified maltopentaose-producing amylase. | amylase a-180, which is secreted by a new alkaliphilic organism, isolate 163-26, consists of a single type of polypeptide chain of 186.5 kda and hydrolyses starch by exo-attack releasing malto-pentaose as preferential product. the structure/function relationship of this unusual starch-degrading enzyme was analysed by introducing 3' deletions into the structural gene. it was found that removal of up to a 110-kda portion from the c-terminus leaving 563 n-terminal amino acids still led to the forma ... | 1991 | 1714389 |
| [the etiologic structure of acute intestinal infections caused by opportunistic microorganisms (irkutsk, 1984-1988)]. | the authors analyze the results of bacteriologic studies carried out in patients with intestinal infections at the laboratory of the sanitary epidemiologic center of the town of irkutsk over 1984-1988. the records of 24,490 case histories show infections induced by opportunistic microflora in 37 percent of cases. the most frequent etiologic agents were staphylococcus aureus and enterobacteriaceae, genera: proteus, enterobacter, citrobacter, klebsiella, hafnia. analysis of the incidence of acute ... | 1991 | 1715437 |
| differentiation of enterobacteriaceae, pseudomonas aeruginosa, and bacteroides and haemophilus species in gram-stained direct smears. | the accuracy of examination of the gram-stained direct smear to classify presumptively gram-negative rods into three morphotype groups, that is, (a) enteric bacteria, (b) pseudomonas, and (c) bacteroides or haemophilus, was evaluated. randomly selected clinical strains (4-9) each of escherichia coli, klebsiella pneumoniae, proteus mirabilis, ps. aeruginosa, b. fragilis, and h. influenzae were used to produce peritonitis or subcutaneous abscesses in mice. a gram-stained direct smear was prepared ... | 1991 | 1716190 |
| evolution of the ferric enterobactin receptor in gram-negative bacteria. | using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ... | 1991 | 1717434 |
| [an evaluation of methods of determining the hemolytic activity of klebsiella in solid nutrient media]. | hemolytic activities of klebsiella-237 strains of various origins were measured in solid nutrient media by three methods making use of six different agar bases for the preparation of blood nutrient media. the authors analyze the agar bases, discuss the advantages and shortcomings of all the methods used with consideration for the purpose of the investigation. the findings evidence a higher hemolytic activity in klebsiella isolated from patients as against the hemolytic activities of reference st ... | 1991 | 1717756 |
| capsular and somatic antigens of klebsiella bacilli. | capsular and somatic antigens were determined in 100 kl. oxytoca strains isolated from patients with the respiratory tract inflammations. k antigens were assigned by the capsula swelling test using 77 specific anti-capsular sera. most frequent were: k14, k2, k55, k8 and k16 antigens. positive reaction was noted with 64 strains in 2 or more sera. somatic antigens of klebsiella oxytoca bacilli were tested by the test tube agglutination reaction. of 63 strains tested with anti-01 kl. pneumoniae and ... | 1990 | 1718235 |
| cloning and nucleotide sequence of the gene (cita) encoding a citrate carrier from salmonella typhimurium. | a cryptic citrate transport gene (cita) from salmonella typhimurium chromosome was cloned and its nucleotide sequence was determined. the cloned plasmid conferred citrate-utilizing ability on wild-type escherichia coli, which cannot grow on citrate as the sole source of carbon. the resultant e. coli transformant was able to transport citrate. a 1,302-base-pair open reading frame with a preceding ribosomal binding site was found in the cloned dna fragment. the 434-amino-acid protein that could be ... | 1991 | 1718953 |
| granulocyte colony-stimulating factor enhances pulmonary host defenses in normal and ethanol-treated rats. | ethanol suppresses functions of the polymorphonuclear leukocyte (pmnl), seriously compromising normal host defenses against pneumonia. because granulocyte colony-stimulating factor (g-csf) augments the number and function of pmnl, the effect of g-csf on the antibacterial defenses of the lung in normal and acutely intoxicated rats was studied. animals received g-csf or vehicle twice a day for 2 days, then ethanol or saline, followed by challenge with klebsiella pneumoniae. k. pneumoniae elicited ... | 1991 | 1719103 |
| infective endocarditis in children in the guinea savannah of nigeria. | thirty-two children with 33 episodes of infective endocarditis were admitted into the paediatric unit of ahmadu bello university teaching hospital, zaria during an 8-year period (january 1982-december 1989). thirty (94%) had underlying heart disease. rheumatic heart disease was the pre-existing anomaly in 21 (66%) while congenital cardiac anomalies were detected in nine (28%). cardiac failure, changing murmur or persisting fever drew attention to the disease. bacterial isolation was achieved in ... | 1991 | 1719922 |
| effect of an immunostimulatory substance of klebsiella pneumoniae on inflammatory responses of human granulocytes, basophils and platelets. | ru 41740 (biostim), a biological response modifier of bacterial origin obtained from the 01.k2 strain of k. pneumoniae, was studied with regard to its effect to modulate the chemiluminescence response, phagocytosis and leukotriene formation from polymorphonuclear leukocytes (pmns), the histamine release from basophils and the aggregation of human platelets. for histamine release human basophils were analyzed with either the calcium ionophore a23187 or hemolysin producing bacteria. ru 41740 reduc ... | 1991 | 1723598 |
| immunobiology of hematopoietic colony-stimulating factors: potential application to disease prevention in the bovine. | colony-stimulating factors are a family of glycoproteins instrumental in regulation of hematopoiesis and inflammation. clinical effects of various colony-stimulating factors have been reported in murine and human hosts. this review summarizes findings from some clinical trial evaluations of macrophage colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, interleukin-1, interleukin-3, interleukin-4, interleukin-5, interleukin-6, and in ... | 1991 | 1724001 |
| [a rapid method of bacteriocin typing of microorganisms]. | the technique of making paper disks with klebsiella bacteriocins is described, as is the method for improving the stability of bacteriocin preparations. the authors have developed a method for bacterial bacteriocin typing, facilitating the work and taking but 4-5 hrs. the results of klebocin typing of klebsiella-237 strains with the use of the developed technique are presented. | 1991 | 1724041 |
| drug treatment of pneumonia in the hospital. what are the choices? | mortality and morbidity of nosocomial pneumonia remain high. successful treatment of pulmonary infections depends on several factors including type of infection, offending pathogen, status of host defences, and adequate choice of antibiotic therapy. the physician's decision should aim at achieving antibiotic concentrations beyond the mic at the site of infection. gram-negative bacilli, notably pseudomonos aeruginosa, klebsiella pneumoniae and escherichia coli, remain the most frequent agents in ... | 1991 | 1724642 |
| review of clinical experience in the united states with cefpodoxime proxetil in adults with uncomplicated urinary tract infections. | two controlled united states trials compared the safety and efficacy of cefpodoxime proxetil (100mg twice daily) with either cefaclor (250mg 3 times daily) or amoxicillin (250mg 3 times daily) in patients with uncomplicated urinary tract infections. treatment duration was 7 days. 307 of 762 patients treated with cefpodoxime proxetil, 99 of 190 treated with cefaclor, and 57 of 185 treated with amoxicillin were evaluable for efficacy. 311, 99 and 59 pathogens were isolated from cefpodoxime proxeti ... | 1991 | 1726207 |
| molecular epidemiology of klebsiella pneumoniae. | plasmid profile, restriction fragment length polymorphism (rflp) patterned by an rrna probe, and capsule serotyping of 143 taiwan local bacteriemic isolates of klebsiella pneumoniae were analyzed. our results indicate that k1 and k2 are probably the most prevalent serotypes in taiwan, accounting for 39% of total isolates tested. a remarkable genotypic heterogeneity was observed among these isolates by the analysis of rrna gene rflp patterns and plasmid profile, which highlighted differences that ... | 1991 | 1726368 |
| human monoclonal antibodies to glycolipid a that exhibit complement species-specific effector functions. | two human igm monoclonal anti-glycolipid a antibodies (mabs) were evaluated for their abilities to bind to various endotoxins and pathogenic gram-negative bacteria and to activate complement pathways, thereby accomplishing bactericidal and opsonic effector functions. both mabs cross-reacted with glycolipid a mutant lipopolysaccharides from rough colony-forming gram-negative bacteria and with selected endotoxins from smooth colony-forming bacteria. however, mab 10235 bound to all clinical isolate ... | 1992 | 1727895 |
| etiology and diagnosis of pneumonia requiring icu admission. | the spectrum of pathogens and the microbiologic investigations used to obtain a diagnosis in 178 patients with severe pneumonia (88 percent requiring intermittent positive-pressure ventilation) are reviewed. ninety-five patients had primary pneumonia, 31 had nosocomial pneumonia, 24 were immunocompromised patients, and 28 had aspiration pneumonia. while the spectrum of isolates conformed to the usual patterns for the different types of pneumonia, the incidence of gram-positive infections (15 per ... | 1992 | 1729070 |
| free versus liposome-encapsulated muramyl tripeptide phosphatidylethanolamide in treatment of experimental klebsiella pneumoniae infection. | the effect of free and liposome-encapsulated muramyl tripeptide phosphatidylethanolamide (mtppe) on resistance to klebsiella pneumoniae infection in mice was investigated. it was shown that administration of mtppe, at 24 h before bacterial inoculation, led to a dose-dependent antibacterial resistance in terms of increased clearance of bacteria from the blood and bacterial killing in various organs. the lowest effective dose of mtppe was 50 micrograms per mouse. administration of liposome-encapsu ... | 1992 | 1729201 |
| distribution of an l-isoaspartyl protein methyltransferase in eubacteria. | a protein carboxyl methyltransferase (ec 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. this enzyme catalyzes the transfer of methyl groups from s-adenosylmethionine to the carboxyl groups of d-aspartyl or l-isoaspartyl residues that are formed spontaneously from normal l-aspartyl and l-asparaginyl residues. a similar methyltransferase has been found in two bacterial species, escher ... | 1992 | 1729230 |
| completion of the nucleotide sequence of the 'maltose b' region in salmonella typhimurium: the high conservation of the malm gene suggests a selected physiological role for its product. | we have subcloned and sequenced the genes malf and malm of salmonella typhimurium, thereby completing the determination of the nucleotide sequence of its 'maltose b' regulon. the malm gene, encoding a periplasmic protein of unknown function in escherichia coli, is a highly conserved as genes encoding proteins of known function from the same region. | 1992 | 1730061 |
| effects of growth factors, hormones, bacterial lipopolysaccharides, and lipotechoic acids on the clonal growth of normal ureteral epithelial cells in serum-free culture. | in vitro tissue culture techniques were employed to study the effects of bacterial endotoxins on the growth of normal epithelial cells from the human ureter (nhu). primary cultures of nhu cells were initiated from explant outgrowth cultures of human ureteral tissue and cultured on collagen gel in f-12* medium containing 1% fetal calf serum (fcs). optimal clonal growth of secondary cultures of nhu cells seeded at relatively low seeding cell densities, directly on plastic dishes, was achieved in f ... | 1992 | 1730786 |
| parenteral followed by oral ofloxacin for nosocomial pneumonia and community-acquired pneumonia requiring hospitalization. | we report a multicentric, open trial of intravenous followed by oral ofloxacin, 400 mg every 12 h, as therapy for 100 cases of nosocomial pneumonia and community-acquired pneumonia requiring hospitalization. the typical subject was 57 yr old, and underlying diseases, such as chronic obstructive pulmonary diseases (copd), diabetes mellitus, and congestive heart failure, were common. for 10 subjects previous therapy had failed. there were 118 pathogens isolated in blood or sputum; s. pneumoniae wa ... | 1992 | 1731595 |
| transport of 4-hydroxyphenylacetic acid in klebsiella pneumoniae. | klebsiella pneumoniae m5a1 has been shown to possess an inducible transport system for 4-hydroxyphenylacetate (4-hpa). this transport system has a kt of 16.3 microm and a maximal velocity of 31.2 nmol/min (milligrams dry weight). the transport system has been inhibited by inhibitors of energy metabolism with a concomitant decrease in cellular atp concentrations, and the 4-hpa binding activity has been detected in the crude shock extracts. all these observations indicate that 4-hpa uptake is an a ... | 1992 | 1731619 |
| involvement of rcsb in klebsiella k2 capsule synthesis in escherichia coli k-12. | escherichia coli k-12 harboring a part of the structural genes for the klebsiella k2 capsular polysaccharide (cpsk*) expresses a large amount of k2 capsular polysaccharide as a thick capsule in the presence of plasmids carrying rmpa and rcsb. we have previously shown that expression of the klebsiella k2 capsule in e. coli hb101 harboring cpsk* depends on the presence of rmpa, a regulatory gene from a large plasmid of klebsiella pneumoniae chedid (o1:k2). e. coli k-12 jm109, however, produces onl ... | 1992 | 1732199 |
| bacteraemia in children in the south-western cape. a hospital-based survey. | during 1989, of the 8,524 children admitted to the paediatric wards of tygerberg hospital, 165 (1.96%) had bacteraemia. the incidence of community-acquired bacteraemias was 1.6% and that of nosocomial bacteraemias 0.5%. the most important community-acquired isolates were streptococcus pneumoniae, staphylococcus aureus and neisseria meningitidis. the most important nosocomial isolates were klebsiella and salmonella spp. both bacteraemia (relative risk (rr) = 2.08) and severe malnutrition (rr = 3. ... | 1992 | 1733031 |
| oral ofloxacin therapy for lower respiratory tract infection. | we made an open, noncomparative evaluation of ofloxacin, 400 mg orally bid for 10 days, in 98 subjects with community-acquired pneumonia or pathogen-confirmed bronchitis. thirty-nine (40%) of the subjects were treated in the hospital and 59 (60%) were treated as outpatients. the mean age of those treated was 56.2 years; 73 (74%) of the subjects either were more than 60 years old or had a history of chronic obstructive pulmonary disease, or both. there were 95 organisms initially isolated in sput ... | 1992 | 1734527 |
| posttranslational modification of klebsiella pneumoniae flavodoxin by covalent attachment of coenzyme a, shown by 31p nmr and electrospray mass spectrometry, prevents electron transfer from the nifj protein to nitrogenase. a possible new regulatory mechanism for biological nitrogen fixation. | a strain of escherichia coli (71-18) that produces ca. 15% of its soluble cytoplasmic protein as a flavodoxin, the klebsiella pneumoniae niff gene product, has been constructed. the flavodoxin was purified using fplc and resolved into two forms, designated kpfldi and kpfldii, which were shown to have identical n-terminal amino acid sequences (30 residues) in agreement with that predicted by the k. pneumoniae niff dna sequence. 31p nmr, electrospray mass spectrometry, uv-visible spectra, and thio ... | 1992 | 1734967 |
| postoperative inflammation following cataract extraction caused by bacterial contamination of the cleaning bath detergent. | five of 16 patients who had uncomplicated cataract extraction with intraocular lens (iol) implantation within a 2.5 day period experienced increased anterior segment inflammation on their first postoperative day. four of these five patients had phacoemulsification and one a planned extracapsular cataract extraction. all had posterior chamber iol implantation in the capsular bag. in all patients, this anterior segment inflammation cleared with topical steroids over two to three weeks with no evid ... | 1992 | 1735855 |
| pullulanase secretion in escherichia coli k-12 requires a cytoplasmic protein and a putative polytopic cytoplasmic membrane protein. | the previously uncharacterized third and fourth genes (pule and pulf) of the pullulanase secretion gene operon of klebsiella oxytoca strain unf5023 are, respectively, predicted to encode a 55 kda polypeptide with a putative nucleotide-binding site, and a highly hydrophobic 44 kda polypeptide that probably spans the cytoplasmic membrane several times. expression of pule in minicells or under the control of a strong bacteriophage t7 promoter resulted in the production of a c. 58 kda cytoplasmic pr ... | 1992 | 1738317 |
| ionic interactions in the nitrogenase complex. properties of fe-protein containing substitutions for arg-100. | a series of azotobacter vinelandii strains have been constructed in which the nitrogenase fe-protein (av2) was altered by substitutions for arg-100. this invariant residue is a likely partner in a salt bridge with the mofe-protein and, in some species, is the site of reversible regulation by adp-ribosylation (pope, m. r., murrell, s. a., and ludden, p. w. (1985) proc. natl. acad. sci. u. s. a. 82, 3173-3177). although we find that arginine is the optimum amino acid, other residues in this positi ... | 1992 | 1740419 |
| incidence of an aminoglycoside 6'-n-acetyltransferase, acc(6')-1b, in amikacin-resistant clinical isolates of gram-negative bacilli, as determined by dna-dna hybridisation and immunoblotting. | seventy amikacin-resistant clinical isolates of gram-negative bacteria belonging to nine genera were examined by immunoblotting and by dna-dna hybridisation for the presence of acc(6')1b enzyme, previously called aac(6')-4, or its encoding gene aaca1b. the organisms mostly had resistance profiles compatible with aac(6') production and were from south and north america, the far east and europe. polyclonal (rabbit) anti-aac(6')-1b antisera and an intragenic aaca1b (aaca4) probe derived from the mu ... | 1992 | 1740788 |
| molecular cloning and nucleotide sequence of a new plasmid-coded klebsiella pneumoniae beta-lactamase gene (shv-2a) responsible for high-level cefotaxime resistance. | patient specimen isolates of klebsiella pneumoniae exhibiting an mic of greater than 128 mg/l for cefotaxime were shown to produce a beta-lactamase with a pi of 7.6, which is encoded on a 66 kb conjugative plasmid. a 3.5 kb bam hi fragment of this plasmid was cloned into plg339 and totally sequenced. the nucleotide sequence of the beta-lactamase gene presented 99% homology to those of shv-2 and shv-3, the deduced amino acid sequence differed from both enzymes in one and two positions, respective ... | 1991 | 1741919 |
| bacterial contamination of three-way taps--one australian hospital's experience. | the incidence and types of organisms present in the vacant ports of 424 three-way taps were studied. the taps were used in 50 children in icu. twenty-five (6%) of the taps showed organism growth of one to three colonies from swabs taken of one or more vacant ports. the organisms cultured were staphylococci, klebsiella, streptococci, bacilli and fungi. while there was no marked difference in the growth rate of organisms in three-way taps used in intravenous, intra-arterial and central venous line ... | 1991 | 1741982 |
| [e. coli strains producing heat labile enterotoxin on vero cells]. | in this study heat-labile toxin (lt) producing strains of enterotoxigenic escherichia coli (etec) have been investigated by using vero cell-line, which were isolated predominantly from the cultures of stool samples of 100 infants, aged between 0-2, with diarrhea. among these strains 3% were found as toxin producers. these 3 strains were also detected as toxin producers and serotyped as o?:h12,lt(+); o?:h53,lt(-); (0126):h27,(lt)(-) at statens serum institute (denmark) international escherichia a ... | 1991 | 1745151 |
| comparison of the sequences of the nage operons from klebsiella pneumoniae and escherichia coli k12: enhanced variability of the enzyme iin-acetylglucosamine in regions connecting functional domains. | the nage operon, encoding the enzyme ii specific for n-acetylglucosamine (eiinag), and adjacent dna from the chromosome of klebsiella pneumoniae were sequenced and compared with the corresponding sequence from escherichia coli k12. the deduced eiinag sequences differ in 72 out of 651 amino acids, the k. pneumoniae sequence being three residues longer. the amino acid differences were distributed unevenly, and were most frequent in regions connecting the three functional domains of the protein. in ... | 1991 | 1745234 |
| metabolic engineering of klebsiella oxytoca m5a1 for ethanol production from xylose and glucose. | the efficient diversion of pyruvate from normal fermentative pathways to ethanol production in klebsiella oxytoca m5a1 requires the expression of zymomonas mobilis genes encoding both pyruvate decarboxylase and alcohol dehydrogenase. final ethanol concentrations obtained with the best recombinant, strain m5a1 (ploi555), were in excess of 40 g/liter with an efficiency of 0.48 g of ethanol (xylose) and 0.50 g of ethanol (glucose) per g of sugar, as compared with a theoretical maximum of 0.51 g of ... | 1991 | 1746941 |
| bacterial infection of hydatid cyst following albendazole treatment. | | 1991 | 1746988 |
| filling defects of infectious origin in the cystogram of a 6-year-old child. | | 1991 | 1747740 |
| molecular mimickry between hla b27 and yersinia, salmonella, shigella and klebsiella within the same region of hla alpha 1-helix. | two new examples of amino acid homology between hla b27 and microbes triggering hla b27-associated diseases are described. an outer membrane protein yada (yersinia adhesin, previously called yop1) of yersinia enterocolitica and y. pseudotuberculosis shares a linear tetrapeptide with hla b27. a cationic outer membrane protein omph of salmonella typhimurium shares homology with five amino acids of hla b27 in a non-linear fashion. the four amino acids of yada are also notably included in the hexape ... | 1991 | 1747948 |
| in vitro activity of ro 09-1428 compared to other cephalosporins. | the in vitro activity of ro 09-1428, a new catechol-type parenteral cephalosporin, was compared to that of ceftazidime, e-1040, cefpirome and cefepime against gram-positive and gram-negative organisms. ro 09-1428 inhibited group a streptococci at less than or equal to 0.12 micrograms/ml, and group b, c and g streptococci and streptococcus pneumoniae at 0.5 micrograms/ml, whereas for staphylococcus aureus ro 09-1428 had mics of 8-16 micrograms/ml similar to ceftazidime and e-1040. against pseudom ... | 1991 | 1748124 |
| in vitro activity of cefcanel versus other oral cephalosporins. | cefcanel is a new orally absorbed cephalosporin. its activity was compared with that of cefuroxime, cefaclor, cephalexin, and cefixime against gram-positive and negative aerobic and anaerobic bacteria. cefcanel had excellent activity against methicillin-susceptible staphylococcus aureus and staphylococcus epidermidis, mic90 1 micrograms/ml, superior to the other oral cephalosporins. however, methicillin-resistant staphylococci were resistant, mic greater than or equal to 16 micrograms/ml. strept ... | 1991 | 1748125 |
| in vitro activity of the novel cephalosporin gr69153. | the in vitro activity of gr69153 was compared to that of ceftazidime, ceftriaxone, imipenem and gentamicin against a total of 702 recent clinical isolates. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(8) cfu) were used throughout. gr69153 inhibited 90% of isolates of escherichia coli, klebsiella pneumonia and proteus mirabilis at less than or equal to 0.25 mg/l and 90% of isolates of pseudomonas aeruginosa at less than or equal to 1 mg/l. citrobacter f ... | 1991 | 1748126 |
| diabetes mellitus and klebsiella pneumoniae liver abscess in adults. | klebsiella pneumoniae hepatic abscess in five diabetic patients is reported. the recognition of a possible association between diabetes mellitus and pyogenic hepatic abscess, especially solitary ones, is highlighted. the clinical presentation including metastatic complications and the need for early diagnosis and institution of therapy are emphasized. needle aspiration under ultrasonography is advocated and culture for anaerobes is stressed. | 1991 | 1750096 |
| bulk purification of isochorismic acid by low-pressure octadecyl (c18) reverse-phase liquid chromatography. | partially purified isochorismate synthase (ec 5.4.99.6) from klebsiella pneumoniae 62-1 was used to produce bulk quantities (3.4-6.8 mg) of isochorismate from chorismate. a new, preparative, low-pressure liquid chromatographic method for the purification of isochorismate was used; a (1.0 x 13.0 cm) octadecyl (c18) reverse-phase column with a discontinuous, stepped methanol gradient as eluent. the recovery of isochorismate was quantitative and its purity was verified by hplc using a butyl (c4) re ... | 1991 | 1750676 |
| bacteriology of diabetic foot ulcers. | seventy five diabetic foot ulcers were cultured using optimal aerobic and anaerobic microbiologic techniques. there were a total of 223 isolates (162 aerobes and 61 anaerobes) representing an average of 2.97 bacterial species per specimen. mixed organisms were the most common isolates. staphylococcus aureus was the commonest isolate being recovered in 60% of cases. anaerobic organisms were isolated in 46 patients (61.33%) and the most frequent anaerobe isolated was bacteroides fragilis. the othe ... | 1991 | 1752646 |
| evaluation of ceruloplasmin in neonatal septicemia. | serial serum ceruloplasmin (cp) levels were estimated in healthy and septicemic neonates, using single radial immunodiffusion. in 25 healthy neonates mean cp levels were 19.82 mg/dl at birth, 18.20 mg/dl at 12-24 hours, 17.26 mg/dl at 14 +/- 4 days and 17.68 mg/dl at 28 +/- 4 days of life. for the entire neonatal period the mean cp levels were computed to be 18.24 mg/dl. in 20 culture positive, septicemic neonates, mean cp levels were 27 mg/dl at onset of disease, 28.65 mg/dl 12-24 hours later a ... | 1991 | 1752676 |
| chemotactic cytokine gene expression and production induced in human monocytes by membrane proteoglycans from klebsiella pneumoniae. | the present study was designed to investigate the effect of membrane proteoglycans (mpg) from klebsiella pneumoniae on production of the chemotactic cytokine, il-8, and monocyte chemotactic protein (mcp) by human peripheral blood monocytes. exposure of human peripheral blood monocytes to mpg in vitro induced high levels of mrna transcripts for il-8 and mcp, as assessed by northern blot analysis. cytokine gene expression was associated with the production of chemotactic activity in the supernatan ... | 1991 | 1752702 |
| examination of host defense factors responsible for experimental chronic respiratory tract infection caused by klebsiella pneumoniae in mice. | we have studied the host defense factors that operate during the course of chronic respiratory tract infection caused by klebsiella pneumoniae 27 in cba/j mice. a large number of polymorphonuclear leukocytes (pmns) rapidly infiltrated the alveolar spaces after infection. treatment with cyclophosphamide (cy) before infection greatly reduced the infiltration of pmns and caused an increase in bacterial counts. cy treatment of mice in the chronic phase also caused bacterial proliferation in the lung ... | 1991 | 1753882 |
| genetic organization of the kpni restriction--modification system. | the kpni restriction-modification (kpni rm) system was previously cloned and expressed in e. coli. the nucleotide sequences of the kpni endonuclease (r.kpni) and methylase (m. kpni) genes have now been determined. the sequence of the amino acid residues predicted from the endonuclease gene dna sequence and the sequence of the first 12 nh2-terminal amino acids determined from the purified endonuclease protein were identical. the kpnir gene specifies a protein of 218 amino acids (mw: 25,115), whil ... | 1991 | 1754388 |
| antibacterial properties of human amniotic membranes. | amniotic membranes are widely used in a multitude of surgical applications and have been shown to reduce bacterial counts and promote healing in infected wounds. antibacterial properties of amniotic fluid are well documented and the presence of many potentially antibacterial factors has been demonstrated. no such factors have yet been found in amniotic membranes. we have applied a direct disc-diffusion susceptibility test to try to establish the possible existence of such a factor. amniotic memb ... | 1991 | 1754577 |
| aminoglycosides versus beta-lactams in gram-negative pneumonia. | newer beta-lactam antibiotics with high levels of activity against gram-negative aerobic bacilli (including pseudomonas aeruginosa) such as cefoperazone, ceftazidime, imipenem, and aztreonam may be suitable for monotherapy of gram-negative pneumonia. aminoglycoside antibiotics (gentamicin, tobramycin, amikacin are also highly active against these same organisms and have been more extensively used, but are both ototoxic and nephrotoxic. key therapeutic questions are whether beta-lactams can safel ... | 1991 | 1754734 |
| vaccina treatment in some hospital infections. | phenol-treated vaccine was prepared from the usual hospital strains and patients with colorectal cancer were immunized intramuscularly 7-10 days prior to the operation. the efficiency of the vaccine treatment was judged by the incidence of wound infections. the best results were obtained in patients whose previously estimated immunoreactivity was strong or normal. immunospecificity could not be detected in the above treatment. the frequent and inappropriate use of antibiotics in hospitals increa ... | 1991 | 1755327 |
| bacteriology of infections in a rural tropical area of kenya: isolates and antibiotic susceptibility. | no microbial data are available for rural tropical areas. yet most people in africa live in rural areas where the burdens of infectious diseases remain the primary cause for morbidity and mortality. a pilot study was done by culturing midstream urine from patient with dysuria (100 with gram-negative fermentative rods), blood from patients with fuo (55/307 positive) and swabs from patients with infected wounds (29 with staphylococcus aureus). bacteria were identified and susceptibility tests were ... | 1991 | 1756701 |
| [two cases of gas-containing liver abscess--review of japanese literature]. | | 1991 | 1758088 |
| pneumopyopericardium in a zimbabwean man with klebsiella pneumonia. | | 1991 | 1759010 |
| [an experimental study of a lactoglobulin preparation against opportunistic bacteria and salmonellae]. | a preclinical study of seven batches of lactoglobulin, a new biological preparation against opportunistic bacteria and salmonellae, has been carried out. high antibacterial activity of the preparation with respect to the virulent forms of salmonella typhimurium, salmonella enteritidis, pseudomonas aeruginosa, klebsiella pneumoniae, proteus vulgaris, proteus mirabilis has been established. the preparation has been shown to be safe and nontoxic. the 4-year term of its storage at a temperature of 6 ... | 1991 | 1759523 |
| use of a broad-host-range gyra plasmid for genetic characterization of fluoroquinolone-resistant gram-negative bacteria. | the gyra genotypes of ciprofloxacin-resistant clinical isolates of escherichia coli (n = 3), klebsiella pneumoniae (n = 4), providencia stuartii (n = 2), pseudomonas aeruginosa (n = 1), and acinetobacter calcoaceticus (n = 1) were analyzed in a dominance test. this test is based on the dominance of a wild-type gyra gene (gyra+) over the quinolone resistance allele (gyra) in a heterodiploid strain. plasmid pbp515, developed to carry the gyra+ gene of e. coli k-12 on a broad-host-range vector deri ... | 1991 | 1759823 |
| comparative study of pharmacokinetics and serum bactericidal activity of ceftizoxime and cefotaxime. | single 2-g intravenous doses of ceftizoxime (czx) and cefotaxime (ctx) were given over 30 min to 10 adult volunteers in a crossover manner on two separate occasions. concentrations of czx, ctx, and the primary metabolite of ctx, desacetylcefotaxime (dctx), in serum, suction-induced-blister fluid, and urine were determined by high-pressure liquid chromatography. pharmacokinetic parameters were estimated by using an extended least-squares modeling program (mkmodel). czx exhibited a half-life in se ... | 1991 | 1759827 |
| antibacterial multifilament nylon sutures. | multifilament nylon fibers were made antibacterial by dopping with iodine. nylon fibers were immersed in acetone solution of iodine for 48 hours at room temperature for dopping of iodine. it was observed that iodine uptake by the nylon fibers increased with the increase in concentration of iodine in the solution. antibacterial activity of these iodine dopped samples was evaluated by measuring the zone of inhibition. the bacterial species used for this study were escherichia coli, pseudomonas aer ... | 1991 | 1760495 |
| evaluation of brl 42715, a beta-lactamase-inhibiting penem. | brl 42715 is a new penem which inhibits a wide range of plasmid as well as chromosomally mediated bacterial beta-lactamases. we used a total of 902 recent clinical isolates, consisting of 455 enterobacteriaceae, 247 staphylococci and 200 other gram-negative bacteria to evaluate its ability for potentiation of amoxycillin. mics for all the 104 strains of methicillin-susceptible staphylococcus aureus were reduced from 8-greater than 32.0 to less than or equal to 0.06 micrograms/ml in the presence ... | 1991 | 1760938 |
| [control of genetic consequences of vaccinations: electron microscopic analysis of murine synaptonemal complexes]. | the ability of two inactivated bacterial vaccines, from proteus vulgaris and klebsiella pneumoniae, to injure synaptonemal complexes (scs) was studied by means of electron microscopy. the preparations were given intraperitoneally to c57bl/6j male mice during five successive days and testes were fixed 24 h after the last injection. cyclophosphamide was used for positive control. the vaccine from klebsiella given at subtoxic dose, which was about 200 times higher that that given to people during v ... | 1991 | 1761213 |
| microbiological exploitation of cardiac glycosides and alkaloids from garcinia kola, borreria ocymoides, kola nitida and citrus aurantifolia. | the four medicinal plants, garcinia kola (roots), borreria ocymoides (leaves), kola nitida (bark) and citrus aurantifolia (roots) were screened for phytochemical components. they were found to contain tannins, phlobatannins, polyphenols, hydroxymethyl anthraquinones, glucides, saponins, alkaloids, cardiac glycosides, flavanoids and reducing compounds. the aqueous and alcoholic extracts as well as alkaloids and cardiac glycosides of the medicinal plants were tested on various pathogenic bacteria. ... | 1991 | 1761433 |
| imipenem resistance associated with the loss of a 40 kda outer membrane protein in enterobacter aerogenes. | an imipenem-resistant strain, enterobacter aerogenes ea-z, was isolated from a blood culture. outer membrane protein (omp) profiles indicated the loss of a 40 kda omp, decreased expression of 42 and 44 kda omps, and increased expression of a 50 kda omp in strain ea-z when compared with imipenem-susceptible clinical isolates of e. aerogenes. the omp profile of ea-z was similar to that of strain ea-si16, an imipenem-resistant e. aerogenes second-step mutant selected on imipenem-containing media. a ... | 1991 | 1761444 |
| comparative efficacy and safety of cefprozil and cefaclor in the treatment of acute uncomplicated urinary tract infections. | cefprozil is a new oral semi-synthetic cephalosporin with broad antibacterial spectrum and prolonged serum elimination half-life. in vitro, cefprozil demonstrates excellent activity against common urinary tract pathogens such as escherichia coli and klebsiella pneumoniae. cefprozil, 500 mg once a day, was compared to cefaclor, 250 mg three times a day, in an open, randomized, comparative, clinical trial for the treatment of acute, uncomplicated, urinary tract infection. one hundred and two adult ... | 1991 | 1761453 |
| identification of the essential cysteine residue in klebsiella aerogenes urease. | during reaction with [14c]iodoacetamide at ph 6.3, radioactivity was incorporated primarily into a single klebsiella aerogenes urease peptide concomitant with activity loss. this peptide was protected from modification at ph 6.3 by inclusion of phosphate, a competitive inhibitor of urease, which also protected the enzyme from inactivation. at ph 8.5, several peptides were alkylated; however, modification of one peptide, identical to that modified at ph 6.3, paralleled activity loss. the n-termin ... | 1991 | 1761535 |
| [neonatal sepsis]. | we noticed coagulase positive staphylococcus is the most common pathogen in the neonatal sepsis in our neonatology unit. we followed 22 cases with neonatal sepsis. blood cultures revealed coagulase staphylococcus in 9 cases, coagulase negative staphylococcus in 6 cases, pseudomonas spp. in 5 cases, e. coli in 1 case, klebsiella spp. in 1 case. the most common symptom was apnea. crp positivity was noted in 15 cases (68.2%) while increase in immature/total neutrophil ratio was observed in 12 cases ... | 1991 | 1762591 |
| [cholecystitis and biliary infection]. | cytobacteriologic study of 150 gallbladder fluid samples obtained before cholecystectomy found no correlations between clinical manifestations, gross and histologic features of the gallbladder, and underlying biliary infection. the potential parts played in these discrepancies by bile, time to surgery, and use of antimicrobial agents are discussed. | 1991 | 1762834 |
| evaluation of a rapid fluorogenic method for the detection of escherichia coli in dairy products. | a rapid fluorogenic medium was evaluated for the detection of escherichia coli in dairy products. the medium was capable of detecting esch. coli after 7.5 h incubation at 41.5 degrees c. samples of pasteurized milk (136), raw milk (63), soft cheese (60) and pasteurized cream (39) were examined with media based on 4-methylumbelliferyl-beta-d-glucuronide (mug-7) and violet red bile agar and there were no significant differences between the numbers of esch. coli detected on the two media. mug-7 med ... | 1991 | 1765595 |
| septicemia in patients with hematological disorders and neutropenia. a retrospective study of causative agents and their resistance profile. | in order to identify the cause of septicemia and the resistance patterns of bacteria in swedish patients with hematological disorders, all positive blood cultures collected at a hematological ward during 1980-1986 were evaluated retrospectively. 198 episodes of septicemia in 129 patients were recorded. 54% were males and 46% women with a median age of 67 years (range 16-88). patients with acute leukemia (46%), lymphoma (19%) and myeloma (19%) dominated. the absolute neutrophil count (anc) was le ... | 1991 | 1767255 |
| resistant strains isolated from bacteremia patients in northern norway. | bacterial isolates from blood cultures in 1985 and 1989 (227 and 258 isolates, respectively), were compared as regards resistance to a series of antimicrobial agents including the more recent beta-lactams and quinolones. an increase in the number of coagulase-negative staphylococcal strains and a decrease in staphylococcus aureus strains were detected, otherwise there were no significant differences in the bacterial patterns in 1985 compared to 1989. except for chloramphenicol, there was no majo ... | 1991 | 1767256 |
| treatment of acute exacerbations of chronic bronchitis: state of the art. | effective treatment of acute bacterial exacerbations of chronic bronchitis (abe) reduces the number of such exacerbations in such patients and may decrease or eliminate background symptoms and improve pulmonary function. the pathologic and physiologic abnormalities of the bronchial system in chronic bronchitis that predispose to bacterial infection probably include impaired mucociliary clearance, obstructed bronchioles, and bacterial infections of the bronchial epithelium. exacerbations of bronc ... | 1991 | 1767808 |
| responsiveness of human monocytes to ru 41.740 (biostim). influence of preincubation in vitro. | biostim, which is a glucoprotein extract of klebsiella pneumoniae, is known to trigger human monocytes to increased secretion of metabolites, which inhibit lymphocyte mitogenesis and which augment nk-activity of lymphocytes. some aspects of this monocyte activation have been examined in this study. the conclusion of the present investigation is that preincubation of human monocytes for 6-24 h in serum-free medium at 37 degrees c renders them unresponsive to biostim, as assessed by secretion of t ... | 1991 | 1768055 |
| growth kinetics of coliform bacteria under conditions relevant to drinking water distribution systems. | the growth of environmental and clinical coliform bacteria under conditions typical of drinking water distribution systems was examined. four coliforms (klebsiella pneumoniae, escherichia coli, enterobacter aerogenes, and enterobacter cloacae) were isolated from an operating drinking water system for study; an enterotoxigenic e. coli strain and clinical isolates of k. pneumoniae and e. coli were also used. all but one of the coliforms tested were capable of growth in unsupplemented mineral salts ... | 1991 | 1768093 |
| amplification, cloning, and sequencing of a nifh segment from aquatic microorganisms and natural communities. | by use of the polymerase chain reaction and degenerate oligonucleotide primers for highly conserved regions of nifh, a segment of nifh dna was amplified from several aquatic microorganisms, including an n2-fixing bacterium closely associated with the marine filamentous cyanobacterium trichodesmium sp., a heterotrophic isolate from the root/rhizome of the seagrass ruppia maritima, and the heterocystous freshwater cyanobacterium anabaena oscillarioides. nifh segments were amplified directly from d ... | 1991 | 1768139 |
| utility of phenomenological models for describing temperature dependence of bacterial growth. | we compared three unstructured mathematical models, the master reaction, the square root, and the damage/repair models, for describing the relationship between temperature and the specific growth rates of bacteria. the models were evaluated on the basis of several criteria: applicability, ease of use, simple interpretation of model parameters, problem-free determination of model parameters, statistical evaluation of goodness of fit (chi 2 test), and biological relevance. best-fit parameters for ... | 1991 | 1768141 |
| [hospital infections in children in a general hospital]. | the following is a summary of what was found after setting into motion an epidemiological surveillance system of nosocomial infections occurring in the pediatrics ward of a 2nd level general hospital. | 1991 | 1768359 |
| isolation and characterization of bacteriophage fc3-10 from klebsiella spp. | fc3-10 is a klebsiella spp. specific bacteriophage isolated on a rough mutant (strain kt707, chemotype rd) of k. pneumoniae c3. the bacteriophage receptor for this phage was shown to be the low-molecular mass lipopolysaccharide (lps) fraction (lps-core oligosaccharides), specifically the heptose content of the lps inner-core. this is the first phage isolated on klebsiella, the receptor for which is the lps-core. this phage was unable to plate on salmonella typhimurium lps mutants with chemotypes ... | 1991 | 1769536 |
| sdd and the novel extended-broad-spectrum beta-lactamases. | | 1991 | 1769935 |
| gram-negative bacterial pneumonia with secondary aspergillosis in an aids patient. | a 40-year-old, hiv-infected female patient received antibiotic treatment for a urinary tract infection. after the initial success of therapy and a symptom-free period, she developed pneumonia with septic shock and adult respiratory distress syndrome (ards). in spite of intensive care and respirator therapy with positive end-expiratory pressure (peep), she died of infectious toxic shock. autopsy findings showed relapsing, gram-negative, bacterial pneumonia (morphologically compatible with klebsie ... | 1991 | 1770754 |
| immunostimulation of neutrophil phagocytic function by ru41740 (biostim) in elderly subjects. | on this randomized, double-blind trial we investigated the effect of ru41740, a glycoprotein extracted from klebsiella pneumoniae, on human neutrophil function after oral administration to elderly subjects with a previously demonstrated phagocytic defect. six subjects were given ru41740 orally at a daily dose of 2 mg for one week the first month and of 1 mg for one week the second month, while six subjects received placebo. already after the first week of treatment with ru41740 (t1) and more evi ... | 1991 | 1772105 |
| [klebsiella pneumonia today]. | analysis of incidence and morphology of klebsiella pneumonia nowadays in the adults (10 observations), children (5 observations) and newborns (23 observations) is presented. the pneumonias account for 11.3% of total number of lobar pneumonias in the adults dying in 1979-1989. klebsiella infection represent 16.1% of total number of autopsies a year according to the pediatric infection pathology department. etiological role of klebsiella is established in 21.5% of total number of autopsies of newb ... | 1991 | 1772357 |
| [common pathogens in burn infection and changes in their drug sensitivity]. | this paper reports common pathogens in burn infection and changes in their drug sensitivity from 1985 to 1988. gram negative rods constituted 55.7% of all pathogens, while 40.5% were gram positive cocci. the former group consisted of 173 strains of pseudomonas aeruginosa (28.5%), 55 proteus (9.1%), 33 serratia (5.4%), 23 klebsiella (3.8%) 19, acinetobacter (3.1%), 18 enterobacter (3.0%) and 17 e. coli (2.8%). among gram-positive cocci, staphylococcus aureus accounted for 32.8%, staphylococcus ep ... | 1991 | 1773380 |
| efficacy and safety of oral cefixime therapy in common infectious diseases in children. | twenty-nine children, including 20 boys and 9 girls, were enrolled in an open trial of oral cefixime therapy for common pediatric infectious diseases. patients aged between 0.5 month and 12 years with a mean +/- standard deviation of 2.3 +/- 2.8 years. the diagnoses included 10 of acute otitis media, 2 of purulent rhinitis, 4 of acute enterocolitis, and 13 of urinary tract infection. most children received 6-12 mg/kg/day of cefixime granules, orally twice daily for more than five days. all 10 ca ... | 1991 | 1776438 |
| peritonitis in children with nephrotic syndrome. | in a retrospective review of 300 children with nephrotic syndrome seen at national taiwan university hospital throughout the 12-year period from 1978 to 1990, 20 episodes of peritonitis were identified in 17 patients (7.4%). gram-negative bacilli (e. coli and klebsiella pneumoniae) were the majority of pathogens, accounting for 55% of all the cases. streptococcus pneumoniae were cultured from only 15% of the patients. an additional 15% had negative culture results, but were clinically responsive ... | 1991 | 1776454 |
| [decreasing oxytetracycline resistance in enterobacteriaceae in veterinary and health conditions after exchange of this substance in animal nutrition]. | the national strategy for the use of chemotherapeutics under which there is a distinction between therapeutics and growth promoters for all uses (human medicine, veterinary medicine, animal nutrition) has resulted in a distinct reduction of the selection pressure and a decrease of the otc resistance among enterobacteriaceae in the eastern german länder (formerly gdr) due to a reduction of otc feeding after 1981 and the final prohibition in 1983. in the following years enterobacteriaceae were inv ... | 1991 | 1777007 |
| serum resistance properties of klebsiella pneumoniae and k. oxytoca isolated from different sources. | a total of 481 k. pneumoniae and k. oxytoca strains from different sources was examined with respect to sensitivity to normal human serum. k. oxytoca strains much more frequently proved to be serum resistant than k. pneumoniae isolates. environmental strains of both species showed greater serum sensitivity than fecal or clinical isolates. while k. oxytoca strains isolated from feces and clinical specimens showed similar frequencies of serum resistant isolates, fecal k. pneumoniae strains less of ... | 1991 | 1777008 |
| a new plate medium for rapid presumptive identification and differentiation of enterobacteriaceae. | a new selective differential agar medium for rapid presumptive identification of enterobacteriaceae from water and food samples is described (emx id agar). by a combination of fluorogenic and chromogenic substrates, the medium detects the presence of beta-d-glucuronidase, beta-d-galactosidase, beta-d-xylosidase, tryptophane deaminase and h2s; additionally, cytochrome-oxidase and indole production can be demonstrated. this medium provides an inexpensive means for simple and rapid presumptive iden ... | 1991 | 1777382 |
| frequency and spectrum of microorganisms isolated from biopsy specimens in chronic colitis. | in 109 patients with chronic diarrhea colonic biopsies were examined for the presence of specific microorganisms. a positive result was obtained in 48% of patients with ulcerative colitis, 50% with crohn's disease, 21% with non-specific colitis and 36% with non-specific proctitis. chlamydiae were found most frequently in all groups. obligate enteropathogenic bacteria were isolated in only three cases of nonspecific colitis. of the facultatively enteropathogenic organisms klebsiella and pseudomon ... | 1991 | 1778136 |