| sers-based sandwich immunoassay using antibody coated magnetic nanoparticles for escherichia coli enumeration. | a method combining immunomagnetic separation (ims) and surface-enhanced raman scattering (sers) was developed to enumerate escherichia coli (e. coli). gold-coated magnetic spherical nanoparticles were prepared by immobilizing biotin-labeled anti-e. coli antibodies onto avidin-coated magnetic nanoparticles and used in the separation and concentration of the e. coli cells. raman labels have been constructed using rod shaped gold nanoparticles coated with 5,5-dithiobis-(2-nitrobenzoic acid) (dtnb) ... | 2010 | 21125089 |
| comparison of (gtg)5-oligonucleotide and ribosomal intergenic transcribed spacer (its)-pcr for molecular typing of klebsiella isolates. | molecular typing of klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. the present study was designed to evaluate poly-trinucleotide (gtg)(5)- and rdna intergenic transcribed spacer (its)-pcr fingerprint analysis for typing of klebsiella pneumoniae and klebsiella oxytoca isolates. multiple displacement amplified dna derived from 19 k. pneumoniae (some with an esbl-phenotype), 35 k. oxytoca isolates, five k. pneumoniae, two k. o ... | 2010 | 21129413 |
| histamine production by enterobacter aerogenes in sailfish and milkfish at various storage temperatures. | enterobacter aerogenes was studied for its growth and ability to promote the formation of total volatile base nitrogen (tvbn) and histamine in sailfish (istiophorus platypterus) and milkfish (chanos chanos) stored at various temperatures from -20 to 37 degrees c. the optimal temperature for bacterial growth in both fish species was 25 degrees c, whereas the optimal temperature for histamine formation was 37 degrees c. the two fish species inoculated with e. aerogenes, when not properly stored at ... | 2005 | 21132980 |
| characterization of six antibacterial response genes from the european corn borer (ostrinia nubilalis) larval gut and their expression in response to bacterial challenge. | six cdnas encoding putative antibacterial response proteins were identified and characterized from the larval gut of the european corn borer (ostrinia nubilalis). these antibacterial response proteins include four peptidoglycan recognition proteins (pgrps), one β-1,3-glucanase-1 (βglu-1), and one lysozyme. tissue-specific expression analysis showed that these genes were highly expressed in the midgut, except for lysozyme. analysis of expression of these genes in different developmental stage sho ... | 2010 | 21167833 |
| antibacterial abietane-type diterpenoid, taxodone from metasequoia glyptostroboides miki ex hu. | in an attempt to isolate bioactive constituents, ethyl acetate cone extract of metasequoia glyptostroboides was subjected to a column chromatographic analysis that resulted in isolation of an abietane-type diterpenoid, taxodone. its structure was elucidated by spectroscopic means. further, taxodone showed potential antibacterial effect as diameters of zones of inhibition against foodborne pathogenic bacteria, such as listeria monocytogenes atcc 19166, salmonella typhimurium kctc 2515, s. enterit ... | 2010 | 21289435 |
| antibacterial and antifungal activities from siamese crocodile blood. | to evaluate the in vitro antimicrobial activity of the siamese crocodile blood against bacteria and fungi. | 2010 | 21294399 |
| an experimentally validated genome-scale metabolic reconstruction of klebsiella pneumoniae mgh 78578, iyl1228. | klebsiella pneumoniae is a gram-negative bacterium of the family enterobacteriaceae that possesses diverse metabolic capabilities: many strains are leading causes of hospital-acquired infections that are often refractory to multiple antibiotics, yet other strains are metabolically engineered and used for production of commercially valuable chemicals. to study its metabolism, we constructed a genome-scale metabolic model (iyl1228) for strain mgh 78578, experimentally determined its biomass compos ... | 2011 | 21296962 |
| production of biogenic amines by lactic acid bacteria and enterobacteria isolated from fresh pork sausages packaged in different atmospheres and kept under refrigeration. | the occurrence of in vitro amino acid activity in bacterial strains associated with fresh pork sausages packaged in different atmospheres and kept in refrigeration was studied. the presence of biogenic amines in decarboxylase broth was confirmed by ion-exchange chromatography and by the presence of the corresponding decarboxylase genes by pcr. from the 93 lactic acid bacteria and 100 enterobacteria strains analysed, the decarboxylase medium underestimates the number of biogenic amine-producer st ... | 2011 | 21316866 |
| in vitro activity of ceftobiprole against frequently encountered aerobic and facultative gram-positive and gram-negative bacterial pathogens: results of the canward 2007-2009 study. | the in vitro activity of ceftobiprole was evaluated against 15 011 clinical isolates obtained from patients in canadian hospitals between 2007 and 2009. all staphylococcus aureus were susceptible to ceftobiprole (mic(90)'s for methicillin-susceptible staphylococcus aureus and methicillin-resistant staphylococcus aureus of = 1 µg/ml and 2 µg/ml, respectively). ceftobiprole was active against penicillin-susceptible streptococcus pneumoniae (mic(90), = 0.06 µg/ml), penicillin-resistant streptococcu ... | 2011 | 21353964 |
| contemporary revision penile prosthesis surgery is not associated with a high risk of implant colonization or infection: a single-surgeon series. | revision penile prosthesis surgery has traditionally been associated with a greater risk of postoperative infection than primary implant placement. this has been attributed to the high prevalence of asymptomatic bacteria found surrounding the implant at the time of revision surgery. | 2011 | 21366878 |
| [the austrian pediatrician theodor escherich as bacteriologist and social hygienist: the 100th anniversary of his death on february 15th, 1911]. | escherich was born on november 29, 1857 in ansbach, bavaria. he has been director of the children hospitals in graz (styria, austria) from 1890 and vienna (austria) from 1902, started his bacteriological investigations in 1884 during a short stay in st. anna children hospital in vienna. this he continued it as an assistant in munich and as clinic director in graz. on the basis of his bacteriological findings in breast-milk, which proved to be sterile, he wanted to detect the physiological and pa ... | 2011 | 21369861 |
| cerebrosides and 2-pyridone alkaloids from the halotolerant fungus penicillium chrysogenum grown in a hypersaline medium. | five new cerebrosides, chrysogesides a-e (1-5), and two new 2-pyridone alkaloids, chrysogedones a and b (6 and 7), were isolated from the fermentation broth of penicillium chrysogenum pxp-55, a halotolerant fungus grown in a hypersaline medium. among them, chrysogesides b-d (2-4) are the first cerebrosides that contain an unsaturated c(19)-fatty acid. their structures were identified by spectroscopic and chemical methods, including cd spectroscopy as well as the modified mosher's method. compoun ... | 2011 | 21381678 |
| resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells. | resveratrol, a nontoxic polyphenol, has been shown to inhibit tumor growth in a xenograft mouse model of neuroblasoma. however, resveratrol is rapidly metabolized, mainly to its glucuronidated and sulfated derivatives. this study demonstrates that resveratrol alone, and not the glucuronidated or sulfated metabolites, is taken up into tumor cells, induces a rise in [ca(2+)](i), and ultimately leads to a decrease in tumor cell viability. a new water-soluble resveratrol formulation was delivered di ... | 2011 | 21401048 |
| substrate specificity of the bacillus licheniformis lyxose isomerase ydae and its application in in vitro catalysis for bioproduction of lyxose and glucose by two-step isomerization. | enzymatic processes are useful for industrially important sugar production, and in vitro two-step isomerization has proven to be an efficient process in utilizing readily available sugar sources. a hypothetical uncharacterized protein encoded by ydae of bacillus licheniformis was found to have broad substrate specificities and has shown high catalytic efficiency on d-lyxose, suggesting that the enzyme is d-lyxose isomerase. escherichia coli bl21 expressing the recombinant protein, of 19.5 kda, s ... | 2011 | 21421786 |
| the activity of antimicrobial peptide s-thanatin is independent on multidrug-resistant spectrum of bacteria. | in this study, the activity of s-thanatin (an analog of antimicrobial peptide derived from thanatin) against different bacterial pathogens frequently which can cause therapeutic problems was tested. the result showed minimal inhibitory concentrations (mics) of s-thanatin against all isolates of the escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterobacter aerogenes, klebsiella ornithinolytica and klebsiella oxytoca were in the range of 4-16µg/ml, no matter which antibiotic the ... | 2011 | 21453736 |
| bacteriologic and plasmid analysis of etiologic agents of conjunctivitis in lagos, nigeria. | background: conjunctivitis, an inflammation of the conjunctiva, is one of the most common eye problems affecting all age groups in nigeria. a better understanding of its epidemiology and the antibiotic susceptibility of etiologic bacterial agents is crucial for the initiation of preventive and therapeutic measures. this study determined the distribution and patterns of bacterial infections in nigerian patients with conjunctivitis. antibiotic resistance patterns and the plasmid profiles of these ... | 2011 | 21484175 |
| enzymatic transesterification of jatropha curcas oil assisted by ultrasonication. | ultrasonication used for the production of fatty acid methyl ester from non-edible vegetable oil using immobilized lipase (chromobacterium viscosum) as a catalyst from enterobacter aerogenes to make the process fully ecologically and environmental friendly. the optimal conditions for biodiesel production is the molar ratio oil to methanol 1:4, catalyst concentration 5 wt.% of oil, reaction time 30min, ultrasonic amplitude 50% (100w/m(3)) and cycle 0.7s. ultrasonication reduce the reaction time c ... | 2011 | 21489849 |
| entropic and enthalpic components of catalysis in the mutase and lyase activities of pseudomonas aeruginosa pchb. | the isochorismate-pyruvate lyase from pseudomonas aeruginosa (pchb) catalyzes two pericyclic reactions, demonstrating the eponymous activity and also chorismate mutase activity. the thermodynamic parameters for these enzyme-catalyzed activities, as well as the uncatalyzed isochorismate decomposition, are reported from temperature dependence of k(cat) and k(uncat) data. the entropic effects do not contribute to enzyme catalysis as expected from previously reported chorismate mutase data. indeed, ... | 2011 | 21504201 |
| metabolic and pharmacokinetic studies of scutellarin in rat plasma, urine, and feces. | aim:to study the metabolic and pharmacokinetic profile of scutellarin, an active component from the medical plant erigeron breviscapus (vant) hand-mazz, and to investigate the mechanisms underlying the low bioavailability of scutellarin though oral or intravenous administration in rats.methods:hplc method was developed for simultaneous detection of scutellarin and scutellarein (the aglycone of scutellarin) in rat plasma, urine and feces. the in vitro metabolic stability study was carried out in ... | 2011 | 21516133 |
| the antibiotic potential of prokaryotic imp dehydrogenase inhibitors. | inosine 5'-monophosphate dehydrogenase (impdh) catalyzes the first committed step of guanosine 5'-monophosphate (gmp) biosynthesis, and thus regulates the guanine nucleotide pool, which in turn governs proliferation. human impdhs are validated targets for immunosuppressive, antiviral and anticancer drugs, but as yet microbial impdhs have not been exploited in antimicrobial chemotherapy. selective inhibitors of impdh from cryptosporidium parvum have recently been discovered that display anti-para ... | 2011 | 21517780 |
| biodegradation of acrylamide by enterobacter aerogenes isolated from wastewater in thailand. | a widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental contamination. fortunately, some microorganisms are able to derive energy from acrylamide. in the present work, we reported the isolation and characterization of a novel acrylamide-degrading bacterium from domestic wastewater in chonburi, thailand. the strain grew well in the presence of acrylamide as 0.5% (w/v), at ph 6.0 to 9.0 and 25 degrees c. identification based ... | 2011 | 21520808 |
| potential application of the nisin z preparation of lactococcus lactis w8 in preservation of milk. | aims: the aim of the study is to evaluate the effectiveness of the preparation of nisin z from lactococcus lactis w8-fermented milk in controlling the growth of spoilage bacteria in pasteurized milk. methods and results: spoilage bacteria isolated from pasteurized milk at 8 and 15°c were identified as enterococcus italicus, enterococcus mundtii, enterococcus faecalis, bacillus thuringiensis, bacillus cereus, lactobacillus paracasei, acinetobacter sp., pseudomonas fluorescens and enterobacter a ... | 2011 | 21554339 |
| bacteremia and endotoxemia after endoscopic submucosal dissection for gastric neoplasia: pilot study. | background: because the invasive procedure of endoscopic submucosal dissection (esd) entails a large mucosal defect which is left open, with extensive submucosal exposure to the indigenous bacterial flora, the procedure may have a substantial risk for bacteremia. our aim was to examine gastric esd-related bacteremia and endotoxemia in gastric neoplasia patients. methods: in patients who underwent esd for superficial gastric neoplasia, blood cultures and plasma endotoxin measurements were done be ... | 2011 | 21559862 |
| comparative evaluation of chromogenic agar cm1046 and mfc agar for detection of e. coli and thermotolerant coliform bacteria from water samples. | aims: the equivalence of oxoid (cm 1046) brilliance((tm)) e. coli/coliform selective agar to mfc agar, as used in the australian/new zealand standard method to detect thermotolerant coliforms and escherichia coli in water samples, was assessed. methods and results: a total of 244 water samples were analysed in parallel over a 5-month period. sewage effluent samples (n = 131, sites = 43), freshwater (n = 62, sites = 18) and marine/brackish water samples (n = 51, sites = 23) were analysed. the w ... | 2011 | 21585405 |
| natural resistance of rose petals to microbial attack. | petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ... | 2011 | 21614712 |
| phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth. | a local herb, kacip fatimah, is famous amongst malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of labisia pumila benth. total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. leaves of var. pumi ... | 2011 | 21623314 |
| development and validation of lc-hrms and gc-nici-ms methods for stereoselective determination of mdma and its phase i and ii metabolites in human urine. | 3,4-methylenedioxymethamphetamine (mdma) is a racemic drug of abuse and its r- and s-enantiomers are known to differ in their dose-response curve. the s-enantiomer was shown to be eliminated at a higher rate than the r-enantiomer most likely explained by stereoselective metabolism that was observed in various in vitro experiments. the aim of this work was the development and validation of methods for evaluating the stereoselective elimination of phase i and particularly phase ii metabolites of m ... | 2011 | 21656610 |
| jacaranda cuspidifolia mart. (bignoniaceae) as antibacterial agent. | abstract this study evaluated, in vitro, the antimicrobial activity of the hexane extract (jche), methanol extract (jcme), and chloroform fraction (jccf) of bark from jacaranda cuspidifolia mart. (family bignoniaceae), a brazilian medicinal plant, traditionally used as anti-syphilis and anti-gonorrhea treatment. the antimicrobial activity was evaluated using the disc diffusion method followed by the determination of minimum inhibitory concentration (mic) values. jche was not active against the b ... | 2011 | 21663482 |
| d-xylulose kinase from saccharomyces cerevisiae: isolation and characterization of the highly unstable enzyme, recombinantly produced in escherichia coli. | the saccharomyces cerevisiae gene encoding xylulose kinase (xks1) was over-expressed to an abundance ofô®¥10% intracellular protein in escherichia coli. instability of xks1, not pointed out in previous reports of the enzyme, prevented isolation of active enzyme in native or "tagged" form under a wide range of purification conditions. a fusion protein haboring c-terminal strep-tag ii (xks1-strep) displayed activity (ôê╝20u/mg) as isolated. however, the half-life time of purified xks1-strep was on ... | 2011 | 21664974 |
| identification and characterization of a re-citrate synthase in dehalococcoides strain cbdb1. | the genome annotations of all sequenced dehalococcoides strains lack a citrate synthase although physiological experiments have indicated that such an activity should be encoded. we here report that a re-face specific citrate synthase is synthesized by dehalococcoides strain cbdb1 and that this function is encoded by the gene cbdba1708 (ncbi accession number cai83711), previously annotated as homocitrate synthase. gene cbdba1708 was heterologously expressed in e. coli and the recombinant enzyme ... | 2011 | 21784924 |
| cytotoxic bipyridines from the marine-derived actinomycete actinoalloteichus cyanogriseus wh1-2216-6. | five new bipyridine alkaloids (1-5) and a new phenylpyridine alkaloid (6), which we name caerulomycins f-k, along with five known analogues (7-11), were isolated from the marine-derived actinomycete actinoalloteichus cyanogriseus wh1-2216-6. the structures of 1-6 were established on the basis of spectroscopic analyses and chemical methods. compounds 1-10 showed cytotoxicity against the hl-60, k562, kb, and a549 cell lines, with ic(50) values of 0.26 to 15.7 µm. compounds 7 and 8 also showed anti ... | 2011 | 21770434 |
| bioengineering of the enterobacter aerogenes strain for biohydrogen production. | enterobacter aerogenes is one of the most widely-studied model strains for fermentative hydrogen production. to improve the hydrogen yield of e. aerogenes, the bioengineering on a biomolecular level and metabolic network level is of importance. in this review, the fermentative technology of e. aerogenes for hydrogen production will be first briefly summarized. and then the bioengineering of e. aerogenes for the improvement of hydrogen yield will be thoroughly reviewed, including the anaerobic me ... | 2011 | 21764301 |
| essential oils from moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant gram-negative bacteria. | bacterial drug resistance is a worrying public health problem. antibiotic efflux is a major non-specific resistance mechanism used by bacteria, and efflux pumps are involved in the low-level susceptibility of various important gram-negative pathogens. use of molecules that can block bacterial pumps is an attractive strategy, but several studies report only partial efficacy owing to limits of these molecules (stability, selectivity, bioavailability, toxicity, etc.). the objective of this study wa ... | 2011 | 21752605 |
| genotypic characterization and in vitro activities of tigecycline and polymyxin b for enterobacteriaceae with decreased susceptibility to carbapenems. | carbapenam-resistant enterobacteriaceae is increasing. in order to evaluate the effects of tigecycline and polymyxin b against the carbapenam-non-susceptible pathogens, 89 representative clinical isolates of carbapenem-non-susceptible enterobacteriaceae were recovered from 7 hospitals of 4 cities in china during 2006 to 2009, including 30 serratia marcescens, 35 klebsiella pneumoniae, 7 enterobacter cloacae, 6 enterobacter aerogenes, 5 escherichia coli, 4 citrobacter freundii, and 2 klebsiella o ... | 2011 | 21835972 |
| antiarthritic and antioxidant effects of the leaf extract of ficus exasperata p. beauv. (moraceae). | leaf extracts of ficus exasperata p. beauv. (moraceae) are commonly used in ghanaian traditional medicine for the treatment of several pathological states including inflammatory disorders. the present study was undertaken to evaluate the antiarthritic effect of an ethanolic extract of f. exasperata (fee) in the freund's adjuvant-induced arthritis model in rats. since free radicals and reactive oxygen species are implicated in inflammatory joint diseases such as rheumatoid arthritis, the antioxid ... | 2010 | 21808547 |
| aromatic compounds from the halotolerant fungal strain of wallemia sebi pxp-89 in a hypersaline medium. | a new cyclopentanopyridine alkaloid, 3-hydroxy-5-methyl-5,6-dihydro-7h-cyclopenta[b]pyridin-7-one (1), together with 11 known aromatic compounds were isolated from the secondary metabolites of the halotolerant fungal strain wallemia sebi pxp-89 in 10% nacl. their structures including the absolute configurations of (2s,3s)-1-(4-hydroxyphenyl)butane-2,3-diol (2), (2r,3s)-1-(4-hydroxyphenyl)butane-2,3-diol (3), and (s)-3-hydroxy-4-(4-hydroxyphenyl)-2-one (4) were elucidated by spectroscopic analysi ... | 2011 | 21725811 |
| antagonistic effect of lactobacillus strains against gas-producing coliforms isolated from colicky infants. | infantile colic is a common disturb within the first 3 months of life, nevertheless the pathogenesis is incompletely understood and treatment remains an open issue. intestinal gas production is thought to be one of the causes of abdominal discomfort in infants suffering from colic. however, data about the role of the amount of gas produced by infants' colonic microbiota and the correlation with the onset of colic symptoms are scanty. the benefit of supplementation with lactobacilli been recently ... | 2011 | 21718486 |
| molecular characteristics of extended-spectrum +¦-lactamases in clinical isolates of enterobacteriaceae from the philippines. | +¦-lactamases, including extended-spectrum +¦-lactamases (esbls) and ampc +¦-lactamases, are major resistance mechanisms of enterobacteriaceae. emergence of plasmid-mediated quinolone resistance (pmqr) determinants in esbl-producing isolates poses a global threat. the molecular characterisitcs of esbl and pmqr determinants in the philippines are not well characterized. in this study, we investigated esbls and ampc +¦-lactamases in clinical isolates of enterobacteriaceae from the philippines, and ... | 2011 | 21820398 |
| detection of bacteria in normal adult nasal cavity based on polymerase chain reaction-denaturing gradient gel electrophoresis. | this study investigated the bacterial diversity in a normal adult nasal cavity using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) of 16s ribosomal rna (rrna) gene fragments and compared the results with those of a culture-based method. | 2011 | 21711966 |
| antimicrobial screening of ethnobotanically important stem bark of medicinal plants. | the stem barks are the rich sources of tannins and other phenolic compounds. tannins inhibited the growth of various fungi, yeast, bacteria and virus. hence, ten stem barks of ethnomedicinally important plants were screened for antibacterial and antifungal activities against human pathogenic strains. | 2010 | 21808577 |
| Potential activity of the purine compounds caffeine and aminophylline on bacteria. | Purine compounds are special types of alkaloids. Caffeine and aminophylline are considered the most important members of purines due to their wide use in therapeutics. | 2011 | 21731299 |
| possible evidence of contamination by catechins in deconjugation enzymes from helix pomatia and abalone entrails. | β-glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. in the present study, we found that compounds having the same molecular weights as catechins were present in helix pomatia- and/or abalone entrails-derived β-glucuronidase and sulfatase by liquid chromatography tandem mass spectrometry (lc-ms/ms) with multiple reaction monitoring methods. on the o ... | 2011 | 21821947 |
| quantification of total and specific gram-negative histamine-producing bacteria species in fish using an mpn real-time pcr method. | quantification of histamine-producing bacteria (hpb) is necessary in order to elucidate the role that hpb play in scombrotoxin (histamine) fish poisoning. we report here the evaluation of a real-time pcr method for the quantification of total and specific gram-negative hpb species in fish using a most probable number (mpn) format. the species-specific real-time pcr assay was 100% inclusive for independently detecting morganella morganii, enterobacter aerogenes, raoultella planticola/ornithinolyt ... | 2011 | 21839377 |
| three new compounds from aspergillus terreus pt06-2 grown in a high salt medium. | to investigate the structurally novel and bioactive natural compounds from marine-derived microorganisms under high salinity, the fungus aspergillus terreus pt06-2 was isolated from the sediment of the putian sea saltern, fujian, china. three new compounds, terremides a (1) and b (2) and terrelactone a (3), along with twelve known compounds (4-15) were isolated and identified from the fermentation broth of a. terreus pt06-2 at 10% salinity. among these metabolites, compounds 4 and 15 only produc ... | 2011 | 21892351 |
| directed evolution combined with rational design increases activity of gpdq toward a non-physiological substrate and alters the oligomeric structure of the enzyme. | directed evolution was used to enhance the activity of the glycerophosphodiesterase enzyme from enterobacter aerogenes, gpdq, toward bis(para-nitrophenol) phosphate (bpnpp), a substrate that is frequently used to assay phosphodiesterases. native gpdq has a low level of activity toward bpnpp while the evolved enzymes exhibited k(cat) values that were well over 100 times better while improvements in k(cat)/k(m) of around 500 times were observed along with improved activity we observed a change in ... | 2011 | 21979136 |
| Inter-laboratory comparison of different rapid methods for the detection of bacterial contamination in platelet concentrates. | Background Bacterial contamination of platelet concentrates still represents a major risk in transfusion medicine, and a variety of screening methods have been available to improve the safety of PCs. In the present study, the analytical quality of three different rapid screening methods (BactiFlow flow cytometry, Pan Genera Detection Assay, 23S rRNA RT-PCR) was evaluated in an inter-laboratory comparison in three different German blood services. Methods Samples were inoculated with different b ... | 2011 | 22150778 |
| Function of UreB in Klebsiella aerogenes urease. | Urease from Klebsiella aerogenes is composed of three subunits (UreA-UreB-UreC) that assemble into a (UreABC)(3) quaternary structure. UreC harbors the dinuclear nickel active site, whereas the functions of UreA and UreB remain unknown. UreD and UreF accessory proteins previously were suggested to reposition UreB and increase the level of exposure of the nascent urease active site, thus facilitating metallocenter assembly. In this study, cells were engineered to separately produce (UreAC)(3) or ... | 2011 | 21939280 |
| emergence of vim-1-carbapenemase-producing enterobacter cloacae in tyrol, austria. | the rapid emergence and dissemination of carbapenemase producing enterobacter sp. and other enterobacteriaceae poses a considerable threat to the care of hospitalized patients and to public health. in this study, enterobacter isolates demonstrating decreased susceptibility to carbapenems detected at the division of hygiene and medical microbiology, innsbruck medical university, between january 2006 and december 2010 were tested for blavim-1, blandm-1, blaimp, blakpc and blaoxa-48 using a multipl ... | 2011 | 22194339 |
| membrane permeability, a pivotal function involved in antibiotic resistance and virulence in enterobacter aerogenes clinical isolates. | clin microbiol infect abstract: imipenem-susceptible e. aerogenes isolates exhibiting extended spectrum β-lactamases, target mutations and a basal efflux expression, were identified in five patients. after imipenem treatment, imipenem-intermediate susceptible (imi-i) or resistant (imi-r) isolates emerged in these patients. alteration in porin synthesis and increase in efflux expression were observed in the imi-i isolates whereas complete loss of the porins, lps alteration and efflux overexpressi ... | 2011 | 21883663 |
| Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens. | In Ayurveda, various herbal preparations are clinically used to prevent or cure infectious diseases. Herbal preparations such as Triphala churna, Hareetaki churna, Dashmula churna, Manjistadi churna, Sukhsarak churna, Ajmodadi churna, Shivkshar pachan churna, Mahasudarshan churna, Swadist Virechan churna and Pipramool churna were investigated by preparing their organic solvent extract for antibacterial potential against enteric bacterial pathogens such as Escherichia coli, Staphylococcus aureus, ... | 2011 | 22171288 |
| determining the disinfection of textiles in compressed carbon dioxide using various indicator microbes. | aims: this paper presents a research on the disinfection efficiency of inoculated textile swatches by compressed carbon dioxide, an environmental friendly way to disinfect textiles as opposed to the conventional laundering procedures using water. the disinfection efficiency was determined by using the following microbes inoculated on cotton test fabrics: enterococcus faecium, enterobacter aerogenes and candida albicans. methods and results: the experiments were performed using the high pressur ... | 2011 | 22151328 |
| resveratrol given intraperitoneally does not inhibit the growth of high-risk t(4;11) acute lymphoblastic leukemia cells in a nod/scid mouse model. | the efficacy of resveratrol as a preventive agent against the growth of t(4;11) acute lymphoblastic leukemia (all) was evaluated in nod.cb17-prkdcscid/j mice engrafted with the human t(4;11) all sem cell line. sem cells were injected into the tail vein and engraftment was monitored by flow cytometry. once engraftment was observed, mice were injected intraperitoneally with resveratrol (10 mg/kg body weight) dissolved in dimethylsulfoxide ... | 2011 | 22200740 |
| resveratrol given intraperitoneally does not inhibit the growth of high-risk t(4;11) acute lymphoblastic leukemia cells in a nod/scid mouse model. | the efficacy of resveratrol as a preventive agent against the growth of t(4;11) acute lymphoblastic leukemia (all) was evaluated in nod.cb17-prkdcscid/j mice engrafted with the human t(4;11) all sem cell line. sem cells were injected into the tail vein and engraftment was monitored by flow cytometry. once engraftment was observed, mice were injected intraperitoneally with resveratrol (10 mg/kg body weight) dissolved in dimethylsulfoxide ... | 2011 | 22200740 |
| Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease. | The micro aerophilic pathogen Helicobacter mustelae synthesizes an oxygen-labile, iron-containing urease (UreA2B2) in addition to its standard nickel-containing enzyme (UreAB). An apoprotein form of the iron urease was prepared from ureA2B2-expressing recombinant Escherichia coli cells that were grown in minimal medium. Temperature-dependent circular dichroism measurements of holoprotein and apoprotein demonstrate an enhancement of thermal stability associated with the UreA2B2 metallocenter. In ... | 2011 | 22196017 |
| effect of crude glycerol-derived inhibitors on ethanol production by enterobacter aerogenes. | in this study, ethanol production from pure and crude glycerol using enterobacter aerogenes atcc 29007 was evaluated under anaerobic culture conditions. inhibitory effects of substrate concentrations, ph, and salt concentrations were investigated based on crude glycerol components. ethanol production was performed with pure glycerol concentrations ranging from 5 to 30 g/l to evaluate the effects of substrate concentration and osmotic pressure. the consumed glycerol was 5-14.33 g/l, and the yield ... | 2011 | 21909938 |
| changes in ciprofloxacin resistance levels in enterobacter aerogenes associated to variable expression of the aac(6')-ib-cr gene. | two closely-related enterobacter aerogenes isolates presented a new identical aac(6')-ib-cr genetic environment, including is26. one of them showed lower mic of ciprofloxacin, norfloxacin, tobramycin and amikacin and decreased expression of aac(6')-ib-cr, which might be related to a 12-bp deletion causing a displacement of the -10 box upstream of the aac(6')-ib-cr gene. | 2011 | 22106222 |
| the disinfecting potential of contact lens soutions used by sultan qaboos university students. | this study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in oman. | 2011 | 21969898 |
| allosteric activation via kinetic control: potassium accelerates a conformational change in imp dehydrogenase. | allosteric activators are generally believed to shift the equilibrium distribution of enzyme conformations to favor a catalytically productive structure; the kinetics of conformational exchange is seldom addressed. several observations suggested that the usual allosteric mechanism might not apply to the activation of imp dehydrogenase (impdh) by monovalent cations. therefore, we investigated the mechanism of k(+) activation in impdh by delineating the kinetic mechanism in the absence of monovale ... | 2011 | 21870820 |
| indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance. | introduction: transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. the purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora. methods: microorganisms in pristine and oily soils wer ... | 2011 | 21948132 |
| in vitro susceptibility of nosocomial isolates against a new aminoglycoside isepamicin. | isepamicin is a new aminoglycoside with a spectrum of activity similar to amikacin with an advantage of possessing a high level of stability against different aminoglycoside modifying enzymes. the in vitro susceptibility of nosocomial isolates obtained from patients admitted to the all india institute of medical sciences, new delhi during october-december, 1999 was tested against isepamicin and compared with other aminoglycosides. | 2001 | 21910285 |
| susceptibility of the multi-drug resistant strain of enterobacter aerogenes ea289 to the terpene alcohols from cistus ladaniferus essential oil. | the essential oil (eo) of cistus ladaniferus was separated into non polar, moderately polar and polar fractions by column chromatography. the eo and its fractions were analysed by gas chromatography in combination with retention indices [gc-(ri)] and 13c nuclear magnetic resonance (13c nmr) spectroscopy. a minimum inhibitory concentration (mic) assay was used to evaluate their antibacterial activity against gram-positive and gram-negative pathogens of clinical relevance, including a multi-drug r ... | 2011 | 21922926 |
| isolation and identification of microbes associated with mobile phones in dammam in eastern saudi arabia. | this study was conducted to determine microbial contamination of mobile phones in the city of dammam, in the eastern region of saudi arabia, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures. | 2010 | 22022665 |
| Amine-alkyl derivatives of hydantoin: new tool to combat resistant bacteria. | A series of new 5,5-diphenylhydantoin derivatives with various amine-alkyl terminal fragments at N1-position were synthesized. Then a series of twenty-eight compounds with the same hydantoin scaffold were evaluated for their potency to combat bacterial MultiDrug Resistance (MDR). Intrinsic antibacterial activities were first evaluated. As these compounds showed no direct activity on bacteria, their influence on minimal inhibitory concentration (MIC) of nalidixic acid was tested in two strains of ... | 2011 | 22000919 |
| Stereoselective urinary MDMA (ecstasy) and metabolites excretion kinetics following controlled MDMA administration to humans. | The R- and S-enantiomers of racemic 3,4-methylenedioxymethamphetamine (MDMA) exhibit different dose-concentration curves. In plasma, S-MDMA was eliminated at a higher rate, most likely due to stereoselective metabolism. Similar data were shown in various in vitro experiments. The aim of the present study was the in vivo investigation of stereoselective elimination of MDMA's phase I and phase II metabolites in human urine following controlled oral MDMA administration. Urine samples from 10 partic ... | 2012 | 21983032 |
| Stereoselective urinary MDMA (ecstasy) and metabolites excretion kinetics following controlled MDMA administration to humans. | The R- and S-enantiomers of racemic 3,4-methylenedioxymethamphetamine (MDMA) exhibit different dose-concentration curves. In plasma, S-MDMA was eliminated at a higher rate, most likely due to stereoselective metabolism. Similar data were shown in various in vitro experiments. The aim of the present study was the in vivo investigation of stereoselective elimination of MDMA's phase I and phase II metabolites in human urine following controlled oral MDMA administration. Urine samples from 10 partic ... | 2012 | 21983032 |
| improved hydrogen production under microaerophilic conditions by overexpression of polyphosphate kinase in enterobacter aerogenes. | effects of different microaerophilic conditions on cell growth, glucose consumption, hydrogen production and cellular metabolism of wild enterobacter aerogenes strain and polyphosphate kinase (ppk) overexpressing strain were systematically studied in this paper, using nah(2)po(4) as the phosphate sources. under different microaerophilic conditions, ppk-overexpressing strain showed better cell growth, glucose consumption and hydrogen production than the wild strain. in the presence of limited oxy ... | 2011 | 22112830 |
| Hydrogen production from biodiesel byproduct by immobilized Enterobacter aerogenes. | The recent rapid growth of the biodiesel industry has generated a significant amount of glycerol as a byproduct. As a result, the price of glycerol is currently relatively low, making it an attractive starting material for the production of chemicals with higher values. Crude glycerol can be directly converted through microbial fermentation into various chemicals such as hydrogen. In this study, we optimized immobilization of a facultative hydrogen producing microorganism, Enterobacter aerogenes ... | 2011 | 21915673 |
| genetic characterization of an extended-spectrum ampc cephalosporinase with hydrolysing activity against fourth-generation cephalosporins in a clinical isolate of enterobacter aerogenes selected in vivo. | extended-spectrum ampc cephalosporinases (esacs) have been reported in enterobacteriaceae, pseudomonas aeruginosa and acinetobacter baumannii. here, we characterize a new ampc variant presenting a broadened substrate activity towards fourth-generation cephalosporins, selected in vivo following cefepime treatment for enterobacter aerogenes. | 2012 | 22001269 |
| dissemination of the rmtb gene carried on incf and incn plasmids among enterobacteriaceae in a pig farm and its environment. | to investigate the prevalence and characterization of 16s rrna methylase-producing bacteria in a pig farm and its environment in east china. | 2011 | 21852287 |
| bioremediation of chromium by novel strains enterobacter aerogenes t2 and acinetobacter sp. pd 12 s2. | purpose: this study had an objective to identify the most potent chromium-resistant bacteria isolated from tannery effluent and apply them for bioremediation of chromium in tannery effluents. methods: two such strains (previously characterized and identified by us)-enterobacter aerogenes (ncbi genbank usa accession no. gu265554) and acinetobacter sp. pd 12 (ncbi genbank usa accession no. gu084179)-showed powerful ... | 2011 | 22203402 |
| prevalence of plasmid-mediated quinolone resistance and its association with extended-spectrum beta-lactamase and ampc beta-lactamase in enterobacteriaceae. | we investigated the prevalence of plasmid-mediated quinolone resistance and its association with extended-spectrum beta-lactamase (esbl) and ampc beta-lactamase in enterobacteriaceae. | 2011 | 22016679 |
| antibacterial effect of food additives and detergents against histamine-producing bacteria on food contact material surfaces. | we investigated the antibacterial activity of food additives and detergents against histamine-producing bacteria on food contact material surfaces. based on minimum inhibitory concentration (mic) testing with morganella morganii nbrc3848, raoultella planticola nbrc3317 and enterobacter aerogenes nctc10006, we screened nine food additives and four detergents with relatively high inhibitory potency. we prepared food contact material surfaces contaminated with histamine-producing bacteria, and dipp ... | 2011 | 22200801 |
| biohydrogen production from cheese whey wastewater in a two-step anaerobic process. | cheese whey-based biohydrogen production was seen in batch experiments via dark fermentation by free and immobilized enterobacter aerogenes mtcc 2822 followed by photofermentation of vfas (mainly acetic and butyric acid) in the spent medium by rhodopseudomonas bhu 01 strain. e. aerogenes free cells grown on cheese whey diluted to 10 g lactose/l, had maximum lactose consumption (∼79%), high production of acetic acid (1,900 mg/l), butyric acid (537.2 mg/l) and h(2) yield (2.04 mol/mol lactose; rat ... | 2011 | 22183564 |
| antimicrobial activity of ulopterol isolated from toddalia asiatica (l.) lam.: a traditional medicinal plant. | aim of the study: the leaves of toddalia asiatica (l.) lam. (rutaceae) are widely used in folk medicine in india to treat various ailments. in our earlier communication we have reported the antimicrobial study on the various extracts of the leaves and the isolation and identification of flindersine, a quinolone alkaloid as the major active principle. in the present study, we report the antibacterial and antifungal activities of ulopterol, a coumarin isolated as another major active antimicrobial ... | 2012 | 22265751 |
| antimicrobial activity of food-compatible plant extracts and chitosan against naturally occurring micro-organisms in tomato juice. | background: chitosan (ac) and five hydroalcoholic extracts from lithospermum erythrorhizon (se), rheum palmatum (re), thymus vulgaris (at), lippia citriodora (plx) and a mixture of rosmarinus officinalis, salvia lavandulifolia and thymus mastichina (la) were tested for antimicrobial activity against bacteria, yeasts and filamentous fungi using two broth dilution methods. the effects of adding single extracts on naturally occurring micro-organisms and sensory qualities of raw tomato juice were al ... | 2012 | 22246685 |
| diseases and causes of death in european bats: dynamics in disease susceptibility and infection rates. | bats receive increasing attention in infectious disease studies, because of their well recognized status as reservoir species for various infectious agents. this is even more important, as bats with their capability of long distance dispersal and complex social structures are unique in the way microbes could be spread by these mammalian species. nevertheless, infection studies in bats are predominantly limited to the identification of specific pathogens presenting a potential health threat to hu ... | 2011 | 22216354 |
| in vitro antibacterial activity of 4-phenyl-1-(2-phenyl-allyl)pyridinium bromide: a novel class of pyridinium based antibacterial compounds. | the continuing increase in the incidence of multi drug resistant pathogenic bacteria and shortage of new antimicrobial agents are the prime driver in efforts to identify the novel antimicrobial classes. in vitro antibacterial activity of 4-phenyl-1-(2-phenylallyl) pyridinium bromide was tested against gram positive staphylococcus aureus, streptococcus species, bacillus subtilis, and gram negative klebsiella aerogenes and escherichia coli using disk diffusion method. among them s. aureus showed s ... | 2011 | 23450086 |
| blood infection with enterobacter aerogenes--an unusual cause of portal vein gas. | portal vein gas was once thought of as an invariably fatal condition. now, with the availability of better equipment and expertise, the condition is more frequently diagnosed. a case of fever with rigors is presented and on ultrasound and ct examination was found to have portal venous gas which resolved with adequate antibiotic treatment. blood culture revealed growth of gram negative bacillus; enterobacter aerogenes. patient was investigated further for portal vein gas, and although no other ca ... | 2011 | 22368913 |
| triterpenoid acids and lactones from the leaves of fadogia tetraquetra var. tetraquetra (rubiaceae). | four triterpenoids isolated from the leaves of fadogia tetraquetra var. tetraquetra, 3beta-hydroxy-11alpha, 12alpha-epoxyoleanan-28,13beta-olide (1), 3beta-hydroxyurs-11-en-28,13beta-olide (2), oleanolic acid (3), and ursolic acid (4), were evaluated for their antiviral and antibacterial properties. compound 4 showed potent activity against the semliki forest virus with an ic50 of 14.7 microm, but was also found to be significantly cytotoxic (68% reduction in cell viability after 24 hours exposu ... | 2011 | 22224262 |
| probing interaction of gram-positive and gram-negative bacterial cells with zno nanorods. | in the present work, the physiological effects of the zno nanorods on the gram positive (staphylococcus aureus and bacillus subtilis) and gram-negative (escherichia coli and aerobacter aerogenes) bacterial cells have been studied. the analysis of bacterial growth curves for various concentrations of zno nanorods indicates that gram positive and gram negative bacterial cells show inhibition at concentrations of ~64 and ~256μg/ml respectively. the marked difference in susceptibility towards nanoro ... | 2012 | 23827568 |
| extended spectrum beta-lactamase producing multidrug resistant urinary isolates from children visiting kathmandu model hospital. | a study was conducted to analyze the status of the multidrug resistant (mdr) isolates producing extended spectrum of beta-lactamase (esbl) among the uropathogens infecting children less than 15 years from november 2010 to april 2011 in the bacteriology laboratory, kathmandu model hospital. urine samples received in the laboratory were processed for routine culture. the antimicrobial susceptibility of bacterial isolates was determined following clinical and laboratory standard institute (clsi) re ... | 2012 | 23671965 |
| etiology and antimicrobial susceptibility pattern of bacterial pathogens from urinary tract infection. | urinary tract infection (uti) is the most common infection in both community and hospital patients. in majority of the cases, empirical antimicrobial treatment is practiced before the laboratory results of urine culture. thus, antibiotic resistance may increase in urinary bacterial pathogens due to improper use of drugs. this study was designed to find out the etiological agents of uti and their prevalence, and to determine the antimicrobial susceptibility pattern of the bacterial pathogens isol ... | 2012 | 23671963 |
| design and evaluation of novel antimicrobial and anticancer agents among tetrazolo[1,5-c]quinazoline-5-thione s-derivatives. | the novel heterocyclization of 5-(2-aminophenyl)-1h-tetrazole with potassium ethylxanthogenate or carbon disulfide was proposed. the potassium salt of the tetrazolo[1,5-c]quinazoline-5-thione was subsequently modified by alkylation with proper halogen derivatives to (tetrazolo[1,5-c]quinazolin-5-ylthio)alkyls, n,n-dialkylethylamines, 1-aryl-2-ethanones, 1-(alkyl)aryl-2-ethanols, carboxylic acids, and esters. the structures of all newly synthesized compounds were confirmed by ft-ir, uv-vis, lc-ms ... | 2012 | 23641327 |
| antibacterial activity of halophilic bacterial bionts from marine invertebrates of mandapam-india. | marine ecosystem and its organisms, particularly the invertebrates are recent targets of bioprospecting and mining for a large group of structurally unique natural products encompassing a wide variety of chemical classes such as terpenes, polyketides, acetogenins, peptides and alkaloids of varying structures, having pronounced pharmacological activities. in view of the limited reports on the antibacterials produced by bacteria, isolated from marine sponges, corals and bivalves of indian origin, ... | 2012 | 23626388 |
| cytotoxic (a549) and antimicrobial effects of methylobacterium sp. isolate (eri-135) from nilgiris forest soil, india. | to assess the antimicrobial and cytotoxic effects of methylobacterium sp. isolated from soil sample of doddabetta forest, nilgiris, western ghats of tamil nadu. | 2012 | 23570000 |
| in vitro studies of antimicrobial activity of crude extracts of the indian grasses dhaman (cenchrus ciliaris) and kala-dhaman (cenchrus setigerus). | the aim of present study was to investigate the antimicrobial activity of cenchrus ciliaris and cenchrus setigerus extracts in order to use it as a possible source for new antimicrobial substances against important human pathogens. crude extracts of the stem of cenchrus ciliaris and cenchrus setigerus were evaluated against some medically important pathogens viz. escherichia coli, raoultella planticola, staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, enterobacter aerogenes, can ... | 2012 | 23439788 |
| antimicrobial isothiocyanates from the seeds of moringa oleifera lam. | 4-(alpha-l-rhamnosyloxy)benzyl isothiocyanate (1) and 4-(4'-o-acetyl-alpha-l-rhamnosyloxy)-benzyl isothiocyanate (2) isolated from moringa oleifera seeds were screened for their antibacterial activities against staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, escherichia coli, enterobacter aerogenes, klebsiella pneumoniae, and pseudomonas aeruginosa, and for their antifungal activities against candida albicans, trichophyton rubrum, and epidermophyton floccosum using the disk ... | 2012 | 23413749 |
| the outcome of treating esbl infections with carbapenems vs. non carbapenem antimicrobials. | in india where the prevalence of extended spectrum beta lactamase (esbl) producing organisms among gram negative organisms is 60-70% and ertapenem was unavailable at the beginning of this study, exclusive use of group 2 carbapenems (imipenem and meropenem) for treatment raises issues of cost and development of resistance. therefore the role of non-carbapenem alternatives, chiefly betalactam + betalactamase inhibitors (bl-bli) was explored in this prospective observational study at a private tert ... | 2012 | 23405518 |
| an adaptive response of enterobacter aerogenes to imipenem: regulation of porin balance in clinical isolates. | imipenem (ipm) is a carbapenem antibiotic frequently used in severe hospital infections. several reports have mentioned the emergence of resistant isolates exhibiting membrane modifications. a study was conducted between september 2005 and august 2007 to survey infections due to enterobacter aerogenes in patients hospitalised in a french university hospital. resistant e. aerogenes clinical isolates obtained from patients treated with ipm and collected during the 3 months following initiation of ... | 2012 | 23280442 |
| a biorefinery from nannochloropsis sp. microalga--extraction of oils and pigments. production of biohydrogen from the leftover biomass. | the microalga nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. the microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical co2 extraction. the temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. the best operational conditions to extract 33 g lipids/1 ... | 2012 | 23265815 |
| formations of calcium carbonate minerals by bacteria and its multiple applications. | biomineralization is a naturally occurring process in living organisms. in this review, we discuss microbially induced calcium carbonate precipitation (micp) in detail. in the micp process, urease plays a major role in urea hydrolysis by a wide variety of microorganisms capable of producing high levels of urease. we also elaborate on the different polymorphs and the role of calcium in the formation of calcite crystal structures using various calcium sources. additionally, the environmental facto ... | 2016 | 27026942 |
| imp dehydrogenase: structure, mechanism, and inhibition. | | 2009 | 19480389 |
| a computational analysis of the three isoforms of glutamate dehydrogenase reveals structural features of the isoform ec 1.4.1.4 supporting a key role in ammonium assimilation by plants. | there are three isoforms of glutamate dehydrogenase. the isoform ec 1.4.1.4 (gdh4) catalyses glutamate synthesis from 2-oxoglutarate and ammonium, using nad(p)h. ammonium assimilation is critical for plant growth. although gdh4 from animals and prokaryotes are well characterized, there are few data concerning plant gdh4, even from those whose genomes are well annotated. | 2006 | 17173671 |
| etiology of inflammatory bowel disease: a unified hypothesis. | inflammatory bowel disease (ibd), including both ulcerative colitis (uc) and crohn's disease (cd), emerged and dramatically increased for about a century. despite extensive research, its cause remains regarded as unknown. about a decade ago, a series of findings made me suspect that saccharin may be a key causative factor for ibd, through its inhibition on gut bacteria and the resultant impaired inactivation of digestive proteases and over digestion of the mucus layer and gut barrier (the bacter ... | 2012 | 22553395 |
| a sequential statistical approach towards an optimized production of a broad spectrum bacteriocin substance from a soil bacterium bacillus sp. yas 1 strain. | bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. the present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as bacillus sp. yas 1 after biochemical and molecular identifications. a sequential statistical approach (plackett-burman and box-behnken) was employed to optimize bacteriocin (bac yas 1) production. u ... | 2014 | 25614886 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| clostridium perfringens epsilon toxin binds to membrane lipids and its cytotoxic action depends on sulfatide. | epsilon toxin (etx) is one of the major lethal toxins produced by clostridium perfringens types b and d, being the causal agent of fatal enterotoxemia in animals, mainly sheep and goats. etx is synthesized as a non-active prototoxin form (proetx) that becomes active upon proteolytic activation. etx exhibits a cytotoxic effect through the formation of a pore in the plasma membrane of selected cell targets where etx specifically binds due to the presence of specific receptors. however, the identit ... | 2015 | 26452234 |
| influence of substrate mineralogy on bacterial mineralization of calcium carbonate: implications for stone conservation. | the influence of mineral substrate composition and structure on bacterial calcium carbonate productivity and polymorph selection was studied. bacterial calcium carbonate precipitation occurred on calcitic (iceland spar single crystals, marble, and porous limestone) and silicate (glass coverslips, porous sintered glass, and quartz sandstone) substrates following culturing in liquid medium (m-3p) inoculated with different types of bacteria (myxococcus xanthus, brevundimonas diminuta, and a carbona ... | 2012 | 22447589 |
| total (bio)synthesis: strategies of nature and of chemists. | the biosynthetic pathways to a number of natural products have been reconstituted in vitro using purified enzymes. many of these molecules have also been synthesized by organic chemists. here we compare the strategies used by nature and by chemists to reveal the underlying logic and success of each total synthetic approach for some exemplary molecules with diverse biosynthetic origins. | 2010 | 21495259 |