| on the extracellular accumulation and isolation of d-ribuloxe in cultures of agrobacterium tumefaciens. | | 1965 | 5834715 |
| studies on nucleic acids from healthy and tumorous tomato stem tissue. | | 1965 | 5835626 |
| aminotransferases of agrobacterium tumefaciens. transamination between tryptophan and phenylpyruvate. | | 1964 | 5837443 |
| phosphatidylcholine synthesis in agrobacterium tumefaciens. ii. uptake and utilization of choline. | | 1965 | 5842051 |
| auxotrophic mutation and infectivity of agrobacterium tumefaciens. | | 1965 | 5847803 |
| sensitivity of rhizobium to the action of thiuriam and phenylmercuric acetate. | | 1965 | 5848592 |
| studies on the toxicity of legume seeds towards rhizobium. | | 1965 | 5848598 |
| [contribution to the study of a synthetic medium for the culture of rhizobium]. | | 1965 | 5848604 |
| [influence of the number of rhizobium inoculated, on the production of lucern-fields]. | | 1965 | 5848614 |
| urea and biuret as nitrogen sources for rhizobium spp. | | 1965 | 5852362 |
| on the active transport of sucrose and of 3-keto-sucrose in agrobacterium tumefaciens. | | 1965 | 5855652 |
| on the cytology and synthetic capacities of natural and artificially produced bacteroids of rhizobium leguminosarum. | | 1965 | 5861289 |
| electron microscopy of the bacteroids and root nodules of lupinus luteus. | | 1965 | 5861290 |
| production of gibberellin-like substances by bacteria and actinomycetes. | | 1965 | 5861292 |
| mutations in symbiotic effectiveness in rhizobium trifolii caused by transforming dna and other agents. | | 1965 | 5864889 |
| [studies of the genetic transformation between agrobacterium tumefaciens and rhizobium sp. 3. comparative analysis of the dna from the partners in a transformation]. | | 1965 | 5875029 |
| nitrogen metabolism of some strains of rhizobium japonicum having different nodulating capacities. | | 1965 | 5879501 |
| the influence of ammonium nitrate on the fine structure of nodules of medicago tribuloides desr. and trifolium subterraneum l. | | 1965 | 5883127 |
| dna base composition, flagellation and taxonomy of the genus rhizobium. | | 1965 | 5883700 |
| timing of events in crown-gall tumor development on pinto bean leaves. | | 1965 | 5884148 |
| transformation of streptomycin markers in rough strains of rhizobium lupini. ii. the relation between the derterminant of streptomycin dependence and those for streptomycin resistance and sensitiveness. | | 1965 | 5884229 |
| [re-isolation experiments with nodular bacteria (rhizobium sp.) from soils]. | | 1965 | 5899298 |
| influence of compounds with antitumor activity on crown gall. | | 1966 | 5901877 |
| agrobacterium tumefaciens conn. 3. effect of thermal shock on bacteria in relation to tumorinducing ability. | stonier, tom (manhattan college, bronx, n.y.), robert e. beardsley, lowell parsons, and james mcsharry. agrobacterium tumefaciens conn. iii. effect of thermal shock on bacteria in relation to tumor-inducing ability. j. bacteriol. 91:266-269. 1966.-bacteria heated to 42 c for 30 min exhibit a decrease in tumor-initiating ability without a detectable loss in viability. the thermal shock inhibits subsequent bacterial growth for up to 1.5 hr. as bacterial growth recovers, so does tumor-initiating ab ... | 1966 | 5903095 |
| chromatography of bacteriophages and deoxyribonucleic acids on deae sephadex. | | 1966 | 5909604 |
| inactivation of a rhizobium bacteriophage by ultraviolet radiation of different wave-lengths. | | 1966 | 5909991 |
| dna homology and taxonomy of pseudomonas and xanthomonas. | | 1966 | 5922298 |
| isolation and morphology of temperature agrobacterium tumefaciens bacteriophage. | zimmerer, robert p. (the pennsylvania state university, university park), robert h. hamilton, and christine pootjes. isolation and morphology of temperate agrobacterium tumefaciens bacteriophage. j. bacteriol. 92:746-750. 1966.-lysogeny was detected in 14 strains of agrobacterium tumefaciens among 130 bacterial strains tested with strain b-6 used as the host. partial lysis was observed with 13 additional bacterial strains. morphological studies of five strains showed that the phage had similar f ... | 1966 | 5922546 |
| crown gall tumorigenesis. ii. relations between wound healing and the tumorigenic response. | | 1966 | 5923157 |
| mechanisms of tumor induction in crown gall. i. production and pathogenicity of spheroplasts of agrobacterium tumefaciens. | | 1966 | 5923158 |
| characteristics of agrobacterium tumefaciens auxotrophic mutant infectivity. | lippincott, barbara b. (northwestern university, evanston, ill.), and james a. lippincott. characteristics of agrobacterium tumefaciens auxotrophic mutant infectivity. j. bacteriol. 92:937-945. 166.-mutants of agrobacterium tumefaciens auxotrophic for adenine, methionine, or asparagine are less infectious than the wild-type strain b6 from which they were derived and show increased infectivity on pinto bean leaves when the specific compounds required for growth of the mutants are added to the inf ... | 1966 | 5926761 |
| requirements for and rates of formation of extracellular pseudouridine by agrobacterium tumefaciens. | | 1966 | 5929154 |
| medium supplementation with l- and d-amino acids relative to growth and efficiency of rhizobium meliloti. | | 1966 | 5929155 |
| factors affecting phage-restricting ability in rhizobium leguminosarum strain l4. | | 1966 | 5929161 |
| [on the presence of rhizobium (rhizobium meliloti) in the nodules of alfalfa cultivated in sahara oases (el afiane, southeastern algeria)]. | | 1966 | 5936219 |
| [on the production of biotin and riboflavin by different strains of agrobacterium tumefaciens (smith and town.) conn]. | | 1966 | 5936223 |
| [comparative study of the fat component of agrobacterium tumefaciens and its fixed l forms. ii. variations according to the method of cultivation and study of the phospholipids]. | | 1966 | 5940294 |
| on the biosynthesis of pseudouridine and of pseudouridylic acid in agrobacterium tumefaciens. | | 1966 | 5942965 |
| approach to an improved taxonomy of the genus agrobacterium. | | 1966 | 5953827 |
| fixed l-forms of agrobacterium tumefaciens induced by ultra-violet light radiation. | | 1966 | 5956332 |
| influence of clay minerals on microorganisms. 3. effect of particle size, cation exchange capacity, and surface area on bacteria. | | 1966 | 5963334 |
| [the mode of action of glycine on the inhibition of growth of agrobacterium tumefaciens. i. effects on the metabolism of messenger rna of the strain b6]. | | 1966 | 5965734 |
| the congo red reaction in bacteria and its usefulness in the identification of rhizobia. | | 1966 | 5969335 |
| influence of clay minerals on microorganisms. ii. effect of various clay species, homoionic clays, and other particles on bacteria. | | 1966 | 5969343 |
| microbial decomposition of various fractions of ureaformaldehyde. | | 1966 | 5970036 |
| primary products of symbiotic nitrogen fixation. i. short-term exposures of serradella nodules to 15n2. | | 1966 | 5972843 |
| primary products of symbiotic nitrogen fixation. ii. pulse-labelling of serradella nodules with 15n2. | | 1966 | 5972844 |
| the probable site of nitrogen fixation in root nodules of ornithopus sativus. | | 1966 | 5972860 |
| [on the testing of virulence in agrobacterium tumefaciens]. | | 1966 | 5985353 |
| [survival and infectivity of different strains of rhizobium in various types of soil]. | | 1966 | 5989239 |
| [synthesis of carotene and carotenoids in mutants of rhizobium lupini]. | | 1966 | 5991621 |
| [some properties of bacteria from nodules of hyppophae rhamnoides l]. | | 1966 | 6002931 |
| fungi associated with legume root nodules and their effect on rhizobia. | | 1966 | 6007064 |
| ecological significance of anthocyanins in the seed coats of the phaseolae. | | 1966 | 6007864 |
| metabolism of amino acids in agrobacterium tumefaciens. ii. uptake of l-valine by growing cells. | | 1967 | 6021174 |
| time required for tumour initiation by agrobacterium tumefaciens on pinto bean leaves. | | 1967 | 6032253 |
| ultraviolet-induced changes in the infectivity of agrobacterium tumefaciens. | the infectivity of agrobacterium tumefaciens strain b6 irradiated with short-wavelength ultraviolet light was followed as a function of dose. previously reported enhancements of b6 infectivity by ultraviolet irradiation, in samples inoculated after 1.75 hr of dark incubation at 27 c, or immediately following irradiation, were found to occur most frequently after losses in cell viability of 60% and of 90% or more, respectively. changes in colony-forming ability and tumor-initiating ability with i ... | 1967 | 6032504 |
| electrophoretic properties of fast- and slow-growing species of rhizobium. | | 1967 | 6033735 |
| effect of clover phyllody virus on nodulation of white clover (trifolium repens) by rhizobium trifolii. | | 1967 | 6034037 |
| bacteriocin production in the rhizobia bacteria. | | 1967 | 6034414 |
| rhizobial relationship of the genus phaseolus. i. cross-inoculation performance of different species to r. phaseoli, r. japonicum, and the cowpea organism. | | 1967 | 6036898 |
| purification and properties of 3-ketosucrose-forming enzyme from the cells of agrobacterium tumefaciens. | | 1967 | 6038493 |
| some effects of medium composition and metabolic intermediates on biotin inhibition in a strain of rhizobium japonicum. | | 1967 | 6039199 |
| [effects of boric acid on the crown-gall bacterium, agrobacterium tumefaciens (smith and town) conn]. | | 1967 | 6055850 |
| utilisation of calcium and magnesium and production of acids by rhizobium spp. from some cultivated legumes. | | 1967 | 6056762 |
| enhancement of agrobacterium tumefaciens infectivity by mitomycin c. | the ability of agrobacterium tumefaciens to induce pinto leaf tumors may be enhanced two- to threefold after treatment with mitomycin c. the enhancement may be obtained with either lethal or nonlethal concentrations. with 10-min treatments, an optimal response was obtained with 0.005 mug of mitomycin c per ml in the absence of any change in the number of viable cells. both the tumor induction process and the tumors induced by treated cultures appear qualitatively the same as controls. to account ... | 1967 | 6057802 |
| growth response of nodule bacteria to phytohemogglutanin. | | 1967 | 6062912 |
| numerical taxonomy of some named bacterial cultures. | | 1967 | 6064342 |
| formation of complexes between dna isolated from tobacco crown gall tumours and rna complementary to agrobacterium tumefaciens dna. | | 1967 | 6064642 |
| biological nitrogen fixation. | | 1967 | 6067409 |
| correlation of physiological characteristics with nodulating ability in rhizobium japonicum. | | 1967 | 6068177 |
| a soluble haemoprotein p 450 from nitrogen-fixing rhizobium bacteroids. | | 1967 | 6073054 |
| [action of capsicine on the multiplication and virulence of agrobacterium tumefaciens]. | | 1967 | 6077893 |
| metabolism of amino acids in agrobacterium tumefaciens. 3. uptake of l-pro- line. | | 1967 | 6082570 |
| changes in t-dna methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line. | phenotypic variation of an octopine-type crown gall tumor line resulting from changes in the pattern of t-dna methylation and expression is described. variants that grow as unorganized callus always express t-dna transcripts 1 and 2. in shoot-forming variants (teratomas) only t-dna transcript 4 is expressed. this line also regenerates normal-appearing, rooted plants in which all t-dna expression is suppressed. tissues from these plants require phytohormones for growth in vitro. these plants are ... | 1984 | 6084805 |
| reverse transcription and kinetic complexity analysis of mrna from lupin root nodules. | complementary dna (cdna) synthesis was performed using mrna from lupin root nodules and from lupin uninfected roots as templates. optimal conditions for the synthesis of 700 nucleotides long cdna were established. hybridization kinetics of mrna-cdna were performed in a homologous system from root nodules as well as in a heterologous system. hybridization analysis revealed the presence of four frequency abundance classes within the root nodule mrna population. in order to enrich the cdna populati ... | 1984 | 6085442 |
| a comparison of virulence determinants in an octopine ti plasmid, a nopaline ti plasmid, and an ri plasmid by complementation analysis of agrobacterium tumefaciens mutants. | transposon-insertion mutants with vir- ti plasmids were characterized and then used in complementation experiments. one of the mutants (lba 1517) had a mutation in a newly discovered vir locus called virf. the virf mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant species. also a mutant (lba 1505) was isolated with a mutation somewhere in the bacterial genome but outside the octopine ti plasmid that caused a restriction in host range for tumor ... | 1984 | 6087390 |
| genetic and molecular characterization of the pseudomonas plasmid pvs1. | a restriction map of the 30-kb nonconjugative pseudomonas plasmid pvs1 was constructed. derivatives of pvs1 obtained in vitro by successive deletions were used to localize on the physical map the determinant for resistance to mercuric ions (carried by transposon tn501), the gene(s) encoding sulfonamide resistance, a 1.6-kb region affecting plasmid stability and establishment in p. fluorescens atcc 13525, and a segment required for mobilization of pvs1 by plasmid rp1. the sulfonamide resistance d ... | 1984 | 6087391 |
| [taxonomic position of agrobacterium strains of hospital origin]. | a collection of 31 strains received as agrobacterium tumefaciens and a. radiobacter was subjected to detailed phenotypic and genomic studies. these strains were recovered from plants, soil, water and clinical specimens. type strains of a. tumefaciens, a. radiobacter, a. rhizogenes and a. rubi were also included. the strains were tested for their ability to use 169 organic compounds as sources of carbon and energy. in addition, 11 conventional characters were studied for each strain. relatedness ... | 1984 | 6087709 |
| crown gall oncogenesis: evidence that a t-dna gene from the agrobacterium ti plasmid ptia6 encodes an enzyme that catalyzes synthesis of indoleacetic acid. | stable incorporation of tumor-inducing (ti) plasmid sequences, the t-dna, into the genomes of dicotyledonous plants results in the formation of crown gall tumors. previous genetic studies have suggested that the products of the genes encoding transcripts 1 and 2, which are encoded by the tl-dna region of ptia6, are responsible for inducing the auxin-independent phenotype of crown gall tissues. here we report the construction of a plasmid, pmtlact2, which directs the synthesis of the mr 49,800 po ... | 1984 | 6089175 |
| characterization of rhizobium japonicum hydrogen uptake genes. | recombinant cosmids from a gene library of the dna from hup+ rhizobium japonicum 122des previously have been shown to restore hydrogenase activity when transferred by conjugation into certain hup- mutants of r. japonicum. we generated a restriction map covering 32.2 kilobases of this cosmid dna. at least 25.3 kilobases of the cosmid phu1 were shown to have the same arrangement as those in the genome of strain 122des. analysis of tn5 insertions into the 122des genome indicates that hup-specific s ... | 1984 | 6090382 |
| physical organization of the bradyrhizobium japonicum nitrogenase gene region. | in bradyrhizobium japonicum usda 110 the three genes that encode the nitrogenase enzyme complex are separated into two transcription units, nifh and nifdk. we have physically mapped a 33-kilobase-pair region of the b. japonicum genome that contains both nifh and nifdk. the nifdk operon is located transcriptionally upstream from nifh, and all three genes are transcribed in the same direction. within the 20-kilobase-pair region that separates the promoters for these two transcription units, we hav ... | 1984 | 6090394 |
| comparison of t-dna oncogene complements of agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges. | the t-dna oncogene complements of the limited-host-range tumor-inducing plasmid ptiag63 and the wide-host-range plasmid ptia6 were compared. the resulting data indicate that ptiag63 has dna sequences related to most of the genes encoded by the oncogene region, the tl-dna, of ptia6 and that these sequences are divided between two t-dna regions, the ta-dna, which encoded sequences related to ptia6 genes 4 (the cytokinin independence gene) and 6a, as well as to a ptia6 tl-dna fragment that encoded ... | 1984 | 6090421 |
| host range encoded by the agrobacterium tumefaciens tumor-inducing plasmid ptiag63 can be expanded by modification of its t-dna oncogene complement. | agrobacterium tumefaciens harboring ptia6 incite unorganized tumors on nicotiana rustica, sunflowers, carrots, and tomatoes, whereas isogenic strains of agrobacteria harboring ptiag63 form "rooty" tumors on n. rustica and are essentially avirulent on sunflowers, carrots, and tomatoes. in this report we show that the different host range characteristics of these two plasmids were due, in part, to differences in the t-dna oncogene complements of the plasmids. specifically, we constructed derivativ ... | 1984 | 6090422 |
| conjugal transfer of bacterial chromosomes mediated by the rk2 plasmid transfer origin cloned into transposon tn5. | we report here a novel system for the conjugal transfer of bacterial chromosomes which utilizes the transfer origin (orit) of plasmid rk2 cloned into transposon tn5. tn5 with orit was inserted by transposition into the chromosomes of escherichia coli and rhizobium meliloti. the orit sequence then served as the origin of high-frequency chromosome transfer when a helper rk2 plasmid was present in the same cell. the broad host range features of rk2 make this system of oriented chromosome mobilizati ... | 1984 | 6090433 |
| the t-dna of agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants. | plants regenerated from tobacco hairy root callus cultures were fertilized by self-pollination, and healthy, morphologically normal r1 offspring were obtained. three of these r1 seedlings were analyzed for the presence of t-dna and synthesis of the t-dna-specific compound agropine. all three r1 plants analyzed contained the same full-length t-dna as the parental regenerant, while only two showed agropine synthesis. | 1984 | 6090564 |
| identification and dna sequence of fixz, a nifb-like gene from rhizobium leguminosarum. | previously, several mutants which nodulated peas but which failed to fix nitrogen were isolated following tn5 mutagenesis of prl 1ji, a symbiotic plasmid of rhizobium leguminosarum. two of these alleles, fix52::tn5 and fix137::tn5 were in a region of prl 1ji which hybridized to a probe that contained the nifa gene and the amino-terminal region of the nifb gene of klebsiella pneumoniae. the nitrogen fixation defect of the fix52::tn5 mutant strain was corrected by a 2.0kb fragment of the correspon ... | 1984 | 6091056 |
| t-dna of agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. | phytohormone overproduction in crown gall tumors is due to the expression of several t-dna genes. the data strongly suggest that the tmr gene (transcript 4) is responsible for cytokinin overproduction by encoding dimethylallyl-pyrophosphate:amp dimethylallyltransferase (dma transferase), an enzyme directly involved in cytokinin biosynthesis. cell-free extracts of escherichia coli strains containing the tmr gene from ptia6nc had dma transferase activity. no activity was present in the control str ... | 1984 | 6091129 |
| [properties of potential vectors--derivatives of the broad-host--range plasmid rp4]. | nonconjugative deletion and recombinant derivatives of the rp4 plasmid are constructed. the plasmids can be used as vectors because they have relatively small molecular weights, unique cleavage sites for enzymes ecori, xhoi, bamhi, psti, kpni, bglii, salgi and hindiii (the plasmids prp401 and prp417 having six of these sites), and easily tested phenotypes (tcr, apr and gal+). in addition, all of them retain the broad host range property. also, the plasmid prp420 is a multicopy derivative capable ... | 1984 | 6094306 |
| physical and functional map of an agrobacterium tumefaciens tumor-inducing plasmid that confers a narrow host range. | agrobacterium tumefaciens ag162 induces crown gall disease on an unusually narrow range of host plants. the 231-kilobase ti plasmid which has been shown to determine host range, was subcloned into the vector pvck102. by comparing overlaps of cloned insets, maps were constructed for the restriction endonucleases sali, xhoi, ecori, and kpni. plasmid incompatibility, octopine catabolism, and at least six virulence genes were localized. plasmid incompatibility between ptiag162 and the wide host rang ... | 1984 | 6094476 |
| reiteration of genes involved in symbiotic nitrogen fixation by fast-growing rhizobium japonicum. | by using cloned rhizobium meliloti nodulation (nod) genes and nitrogen fixation (nif) genes, we found that the genes for both nodulation and nitrogen fixation were on a plasmid present in fast-growing rhizobium japonicum strains. two ecori restriction fragments from a plasmid of fast-growing r. japonicum hybridized with nif structural genes of r. meliloti, and three ecori restriction fragments hybridized with the nod clone of r. meliloti. cross-hybridization between the hybridizing fragments rev ... | 1984 | 6094491 |
| molecular cloning and genetic organization of c4-dicarboxylate transport genes from rhizobium leguminosarum. | cosmids containing c4-dicarboxylate transport (dct) genes were identified from a gene bank of rhizobium leguminosarum dna made in the broad-host-range vector plafr1 by their ability to complement r. trifolii dct mutants. the dct genes were further characterized by subcloning, restriction site mapping, and transposon tn5 and tn7 mutageneses. three dct loci were identified within a 5.5-kilobase region of dna, in the order dcta-dctb-dctc. the results suggested that dcta encoded a structural compone ... | 1984 | 6094513 |
| high frequency mobilization of gram-negative bacterial replicons by the in vitro constructed tn5-mob transposon. | a dna fragment of the broad host range plasmid rp4 carrying the cis-acting dna recognition site for conjugative dna transfer between bacterial cells (mobsite) was cloned into the kanamycin-neomycin resistance transposon tn5. using conventional transposon mutagenesis techniques the new transposon, called tn5-mob, can easily be inserted into the host dna of gram-negative bacteria. a host replicon carrying tn5-mob is then mobilizable into any other gram-negative species if the transfer functions of ... | 1984 | 6094969 |
| structural analysis of the genes encoding the molybdenum-iron protein of nitrogenase in the parasponia rhizobium strain anu289. | the genes encoding the molybdenum-iron protein component of nitrogenase (nifd and nifk) have been identified and fully characterised in the parasponia rhizobium strain anu289. the two genes are contiguous and are separated from the gene encoding the fe-protein component of nitrogenase (nifh) by 21 kb of dna. we present the entire dna sequence of the nifd and nifk genes, thus completing the characterisation of the primary structure of the nitrogenase genes in this rhizobium strain. comparison of ... | 1984 | 6095197 |
| binary agrobacterium vectors for plant transformation. | a vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. it utilizes the trans acting functions of the vir region of a co-resident ti plasmid in agrobacterium tumefaciens to transfer sequences bordered by left and right t-dna border sequences into the nuclear genome of plants. the t-region contains a dominant selectable marker gene that confers high levels of resistance to kanamycin, and a lac alpha-complementin ... | 1984 | 6095209 |
| nucleotide sequence of the insertion sequence found in the t-dna region of mutant ti plasmid ptia66 and distribution of its homologues in octopine ti plasmid. | the octopine tumor-inducing (ti) plasmid ptia66 has an insertion mutation in its t region (the dna region incorporated into the plant genome) that results in the slow growth of crown gall tumors. these tumors exhibit hormonal autonomy different from that of the crown gall tumors caused by wild-type ti plasmids. in the present study, the nucleotide sequences of both the dna segment inserted into ptia66 and its target site have been determined. the inserted segment is 2548 base pairs long and has ... | 1984 | 6095299 |
| design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of agrobacterium tumefaciens plasmid ptic58. | the construction of a set of new plasmids that are suitable as general cloning vectors in escherichia coli and agrobacterium tumefaciens is described. plasmid pucd2 is amplifiable in e. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and antibiotic resistance genes. this includes unique sites for kpni, saci, sacii, psti, clai, sali, ecorv, and pvuii and the genes for resistance to kanamycin, tetracycline, ampicillin, ... | 1984 | 6095350 |
| restriction map of an agropine-type ri plasmid and its homologies with ti plasmids. | the ri plasmid of an agropine-type agrobacterium rhizogenes, strain hri, was cloned in a cosmid and mapped with the restriction endonucleases bamhi, ecori, kpni, smai, and xbai. this plasmid is almost identical to pri1855 and pria4b. a study by southern hybridizations of the homologies with octopine ptib6806 and nopaline ptic58 makes it possible to propose the localization of certain functions on this plasmid, such as virulence, agropine catabolism, agropine synthesis, and the origin of replicat ... | 1984 | 6095353 |
| a portable dna sequence carrying the cohesive site (cos) of bacteriophage lambda and the mob (mobilization) region of the broad-host-range plasmid rk2: a module for the construction of new cosmids. | a polylinker dna sequence carrying the cos site of bacteriophage lambda and the mob (orit) region of the incp group plasmid rk2 was constructed. this composite polylinker has ecori sites at both termini and also unique sites for clai, hindiii, psti and xbai. the cos-mob region is portable with the use of ecori or a combination of ecori with clai, hindiii or xbai. another cos-mob cassette was also constructed from which the cos-mob region can be lifted with hindiii, clai or either of these enzyme ... | 1984 | 6099312 |
| a cauliflower mosaic virus promoter directs expression of kanamycin resistance in morphogenic transformed plant cells. | the promoter region of the camv inclusion body protein gene was modified for use in chimeric gene fusions. the modified promoter was used to construct a selectable marker for plant transformation based on the tn 5 kanamycin resistance gene. this chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of agrobacterium tumefaciens. both types of transformation produced kanamycin-resistant cell lines. the resistant cell lines derived from the deoncogeniz ... | 1984 | 6099400 |