| site-directed mutations of t4 helicase loading protein (gp59) reveal multiple modes of dna polymerase inhibition and the mechanism of unlocking by gp41 helicase. | the t4 helicase loading protein (gp59) interacts with a multitude of dna replication proteins. in an effort to determine the functional consequences of these protein-protein interactions, point mutations were introduced into the gp59 protein. mutations were chosen based on the available crystal structure and focused on hydrophobic residues with a high degree of solvent accessibility. characterization of the mutant proteins revealed a single mutation, y122a, which is defective in polymerase bindi ... | 2006 | 16407253 |
| increase in thyroid follicular cell tumors in nelfinavir-treated rats observed in a 2-year carcinogenicity study is consistent with a rat-specific mechanism of thyroid neoplasia. | the carcinogenic potential of nelfinavir mesylate (nelfinavir) was evaluated in a 2-year oral (gavage) study on sprague-dawley rats at dose levels of 0 (control), 0 (vehicle control), 100, 300 and 1000 mg/kg per day. at the end of the treatment, increased incidences of thyroid follicular cell hyperplasia and neoplasms were observed at 300 (males) and 1000 mg/kg per day (both sexes). there were no other treatment-related effects and no tumors at other sites. results from previous studies indicate ... | 2005 | 16408618 |
| [cervical lymph node metastasis of hypopharyngeal carcinoma]. | to investigate the characteristic of cervical lymph node metastasis of hypopharyngeal carcinoma and its influence to the prognosis. | 2005 | 16408732 |
| [salvage surgical approach for patients with post-radiation local recurrent nasopharyngeal carcinoma]. | to evaluate salvage surgical approaches and efficacy for post-radiation local recurrent nasopharyngeal carcinoma (npc). | 2005 | 16408744 |
| cd25 expression is correlated with histological grade and response to denileukin diftitox in cutaneous t-cell lymphoma. | denileukin diftitox (ontak), a recombinant fusion protein of diphtheria toxin and ligand, il-2, binds to the il-2 receptor, is internalized, and causes cell death. denileukin diftitox was approved for the treatment of cutaneous t-cell lymphomas (ctcls) with cd25+ expression. we prospectively stained lesional skin biopsy specimens from 113 mycosis fungoides and sézary syndrome patients for activation markers cd25 and cd30 to correlate expression with clinical tumor-node metastasis (tnm) stage, hi ... | 2006 | 16410787 |
| type 3 deiodinase is critical for the maturation and function of the thyroid axis. | developmental exposure to appropriate levels of thyroid hormones (ths) in a timely manner is critical to normal development in vertebrates. among the factors potentially affecting perinatal exposure of tissues to ths is type 3 deiodinase (d3). this enzyme degrades ths and is highly expressed in the pregnant uterus, placenta, and fetal and neonatal tissues. to determine the physiological role of d3, we have generated a mouse d3 knockout model (d3ko) by a targeted inactivating mutation of the dio3 ... | 2006 | 16410833 |
| association of apoptosis-related microsatellite polymorphisms on chromosome 1q in taiwanese systemic lupus erythematosus patients. | apoptosis is important in the pathogenesis of systemic lupus erythematosus (sle). several genome-wide scan studies have suggested chromosome 1q as a genetic susceptibility locus for sle. this study investigated the association of apoptosis-related genes on chromosome 1q, fas ligand (fasl), interleukin (il)-10 and poly(adp-ribose) polymerase (parp), promoter microsatellite multi-allelic polymorphisms with sle susceptibility and clinical characteristics in taiwan. this study recruited 237 sle pati ... | 2006 | 16412052 |
| outcomes of hand-assisted laparoscopic nephrectomy in technically challenging cases. | to evaluate the outcomes of hand-assisted laparoscopic nephrectomy in patients with significant complicating clinical factors. | 2006 | 16413330 |
| [central venous lines in children: new trends]. | central venous catheterisation under two-dimensional ultrasound (us) guidance has been proved to be quicker and safer than the classical landmark method in both adults and children. in the literature us guidance with sterile dressing of the probe is the 'gold-standard'. another way to use us is simple preoperative us location followed either by blind puncture, either by us guided puncture when difficulties are expected: small infants (<15 kg), small diameter or collapses of the vein, multiple un ... | 2006 | 16414237 |
| selective neck dissection for node-positive necks in patients with head and neck squamous cell carcinoma: a word of caution. | to evaluate the regional recurrence (rr) rate in a consecutive series of patients with node-positive head and neck squamous cell carcinoma (n(+) hnscc) who underwent selective neck dissection (snd) as part of their treatment in a single institution. | 2006 | 16415434 |
| tsh may not be a good marker for adequate thyroid hormone replacement therapy. | the objective of this study was to evaluate parameters of thyroid function and indices of peripheral thyroid hormone action (such as shbg) in patients whose hypothyroidism was considered well controlled under current criteria. eighty-five patients with t4-treated hypothyroidism, 28 of whom had athyria, were compared with 114 normal individuals with the same tsh levels. t3 levels were significantly lower in hypothyroidism although mean t4 and ft4 levels were significantly higher. furthermore, mea ... | 2005 | 16416346 |
| poly(glycoamidoamine)s for gene delivery: stability of polyplexes and efficacy with cardiomyoblast cells. | polymeric vectors have potential as nucleic acid delivery vehicles for novel gene therapy and oligonucleotide treatments for cardiovascular disease. in this report, poly(glycoamidoamine)s that contain four secondary amines and either two or four hydroxyl units in the repeat unit with d-glucarate (d4), meso-galactarate (g4), d-mannarate (m4), and l-tartarate (t4) stereochemistry have been investigated for their pdna-binding affinity, dnase protection effect, and polyplex stability in the presence ... | 2006 | 16417257 |
| a case of bone metastasis of colon cancer that markedly responded to s-1/cpt-11 combination chemotherapy and became curable by resection. | an oral combined fluoropyrimidine anticancer drug, tegafur/gimeracil/oteracil potassium (s-1), has recently been used alone or in combination for colon cancer. | 2006 | 16417646 |
| [effects of abdominal breathing training using biofeedback on stress, immune response and quality of life in patients with a mastectomy for breast cancer]. | this study was to determine the effects of abdominal breathing training using biofeedback on stress, immune response, and quality of life. | 2005 | 16418556 |
| regional differences in anatomical landmarks for placing anterior instrumentation of the thoracic spine in both normal patients and patients with adolescent idiopathic scoliosis. | a retrospective analysis of patients who had magnetic resonance imaging (mri) of the thoracic spine, comparing those with a normal straight spine and those with a right thoracic adolescent idiopathic scoliosis (ais). | 2006 | 16418638 |
| high iodine intake is a risk factor of post-partum thyroiditis: result of a survey from shenyang, china. | the aim of the present study is to obtain the epidemiological data on post-partum thyroiditis (ppt) firstly in chinese women, and to tryto evaluate whether excessive intake of iodine in post-partum women imposes any danger of occurring ppt. sixty hundred and ten pregnant women were involved in the cohort just before delivery. four hundred and eighty-eight (80%) of them accepted taking part in follow-ups more than 6 months post-partum. a blood sample was taken from participants before delivery an ... | 2005 | 16419489 |
| influence of subclinical hypothyroidism and t4 treatment on the prevalence and severity of obstructive sleep apnoea syndrome (osas). | obstructive sleep apnoea (osa) and subclinical hypothyroidism are relatively frequent disorders that may be causally linked. however, discordant results exist on the prevalence and severity of osa in subclinical hypothyroidism. the aim of this study was to compare the prevalence and severity of sleep-disordered breathing in individuals with or without subclinical hypothyroidism, and to investigate the possible effect of levothyroxine treatment on these patients. | 2005 | 16419491 |
| a new method for the 3d measurement of postoperative swelling following orthognathic surgery. | to describe a new method for measuring facial swelling following orthognathic surgery using a 3d laser-scanning device. | 2006 | 16420272 |
| a screening for the occurrence of autoreactive antibodies in sera of pregnant and non-pregnant bitches. | sera of healthy pregnant (group i, n = 11) and non-pregnant (group ii, n = 11) bitches were screened for autoantibodies (aab). in both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. serum was analysed via indirect immunofluorescence (iif) with the canine ana hep-2 screening kit. in all animals, anticytoplasmic aab were detected. utilizing primate-heart substrate slides aab against contractile proteins of the cytoplasm could be observed. the predominating flu ... | 2006 | 16420328 |
| interpretation of scintigraphic findings of oral malignant tumours with a new scanning agent of technetium-99m-hexakis-2-methoxy-isobutyl-isonitrile (tc-99m-mibi). | the purpose of this study was to investigate the usefulness of a new scanning agent of technetium-99m-hexakis-2-methoxy-isobutyl-isonitrile (tc-99m-mibi) for the diagnosis of malignant tumours of the head and neck. | 2006 | 16421260 |
| effect of high-frequency jet ventilation on oxygenation during one-lung ventilation in patients undergoing thoracic aneurysm surgery. | to evaluate the effect of high-frequency jet ventilation (hfjv) and continuous positive airway pressure (cpap) on oxygenation and the shunt fraction (qs/qt) during one-lung ventilation (olv). | 2006 | 16421668 |
| surgery and clinicopathological features of gastric adenocarcinoma involving the esophago-gastric junction. | to clarify the optimal operative procedure for gastric adenocarcinoma involving the esophago-gastric junction (egj), we investigated 49 cases with an upper gastric cancer invading the esophagus who underwent surgical treatment in our department during the period from 1991 to 2000. according to siewert's classification, there were 21 cases with a type ii tumor, and 28 cases with a type iii tumor. twenty-five cases underwent surgery through an abdominal approach only. the remaining 24 cases were o ... | 2005 | 16422172 |
| thyroid-stimulating hormone assessments in a dutch cohort of 620 men with lifelong premature ejaculation without erectile dysfunction. | apart from the involvement of central serotonergic neurotransmission on lifelong premature ejaculation, interference of thyroid function has been speculated. | 2005 | 16422811 |
| a dominant-negative human growth hormone-releasing hormone (ghrh) receptor splice variant inhibits ghrh binding. | ghrh is a hypothalamic peptide that stimulates the synthesis and secretion of gh from pituitary somatotroph cells. the ghrh receptor is a seven-transmembrane g protein-coupled receptor that localizes to the surface of somatotroph cells and binds ghrh. alternative splicing of the ghrh receptor primary transcript at the intron/exon boundary 3' of exon 11 results in inclusion of sequence that is normally intronic. in the human, this inclusion has an in-frame premature stop codon, and this variant m ... | 2006 | 16423869 |
| grand canonical monte carlo simulation of ligand-protein binding. | a new application of the grand canonical thermodynamics ensemble to compute ligand-protein binding is described. the described method is sufficiently rapid that it is practical to compute ligand-protein binding free energies for a large number of poses over the entire protein surface, thus identifying multiple putative ligand binding sites. in addition, the method computes binding free energies for a large number of poses. the method is demonstrated by the simulation of two protein-ligand system ... | 2006 | 16426059 |
| unique regulation of thyroid hormone metabolism during fasting in the house musk shrew (suncus murinus, insectivora: soricidae). | the active hormone, 3,3',5-triiodothyronine (t3) is derived from thyroxine (t4) by the action of iodothyronine 5'-deiodinases (5'-d). by now two types of 5'-d have been identified; type 1 (d1) and type 2 (d2). a relative contribution of these isotypes to the circulating t3 levels in the human remains to be determined whereas a number of reports indicate that, under physiological conditions, d1 plays a major role in maintaining circulating t3 levels in rodents. in both human and rodents, sickness ... | 2006 | 16426605 |
| the control mechanism for lagging strand polymerase recycling during bacteriophage t4 dna replication. | given the polarity of dna duplex, replication by the leading strand polymerase is continuous whereas that by the lagging strand polymerase is discontinuous proceeding through okazaki fragments. yet the respective polymerases act processively, implying that the recycling of the lagging strand polymerase is a controlled process. we demonstrate that the rate of the lagging strand polymerase relative to that of fork movement affects okazaki fragment size and generates ssdna gaps. we show by using a ... | 2006 | 16427006 |
| biomechanics of vertebral level, geometry, and transcortical tumors in the metastatic spine. | metastatic involvement can disrupt the mechanical integrity of the spine, rendering vertebrae susceptible to burst fracture and neurologic damage. fracture risk assessment for patients with spinal metastases is important in considering prophylactic treatment options. stability of thoracic vertebrae affected by metastatic disease has been shown to be dependent on tumor size and bone density, but additional structural and geometric factors may also play a role in fracture risk assessment. the obje ... | 2007 | 16427058 |
| surgical outcomes in patients with t4 gastric carcinoma. | there is controversy about the best therapeutic surgical approach for treatment of patients with t4 gastric cancer. | 2006 | 16427546 |
| structure-function analysis of the kinase-cpd domain of yeast trna ligase (trl1) and requirements for complementation of trna splicing by a plant trl1 homolog. | trl1 is an essential 827 amino acid enzyme that executes the end-healing and end-sealing steps of trna splicing in saccharomyces cerevisiae. trl1 consists of two domains--an n-terminal ligase component and a c-terminal 5'-kinase/2',3'-cyclic phosphodiesterase (cpd) component--that can function in trna splicing in vivo when expressed as separate polypeptides. to understand the structural requirements for the kinase-cpd domain, we performed an alanine scan of 30 amino acids that are conserved in t ... | 2006 | 16428247 |
| combined spinal epidural causes higher level of block than equivalent single-shot spinal anesthesia in elective cesarean patients. | combined spinal epidural (cse) is an established technique for lower segment cesarean delivery. in this study we tested the hypothesis that the spinal block from a cse technique results in a more extensive spread of local anesthetic in the subarachnoid space than the single-shot spinal (sss) technique. we recruited 30 asa physical status i parturients admitted for elective lower segment cesarean delivery into our randomized, controlled, double-blind study. all patients intrathecally received 2 m ... | 2006 | 16428564 |
| [pathological feature and management of occult lymphatic metastasis in supraglottic carcinoma]. | to study the pathologic feature and management methods of occult lymphatic metastasis in patients with supraglottic carcinoma. | 2005 | 16429726 |
| lack of association between serum tsh or free t4 and body mass index in euthyroid subjects. | there is ongoing debate regarding the influence of minor changes in thyroid status within the normal range and body mass index (bmi). overt thyroid dysfunction is well recognized to affect weight, but the influence of minor perturbations of thyroid function remains unclear. | 2006 | 16430708 |
| thyroid and hypoxic stress in the newt triturus carnifex. | when specimens of the newt triturus carnifex, under anaesthesia by submersion in a 0.2% chlorbutol solution for 25 min, are isolated in a respiratory chamber at 18 degrees c containing water with only 1.3 ppm of oxygen, they consume the oxygen completely in about 3 hr, but they can stay alive for many more hours and wake up with no apparent exterior consequences. hypoxia induces rapid onset of hepatic steatosis and melanosis, as well as a controlled haemolytic process involving a pool of red blo ... | 2006 | 16432885 |
| effects of the antithyroid agent propylthiouracil in a partial life cycle assay with zebrafish. | some ubiquitous pollutants of the aquatic environment, such as pcbs or other polyhalogenated aromatic hydrocarbons, may disrupt the thyroid hormone system. in a partial life cycle assay with zebrafish (danio rerio), we studied the effects of the reference compound propylthiouracil (ptu) on reproduction, growth and development, histopathology of some target tissues, and plasma thyroid hormone levels. ptu induced a concentration-dependent increase of egg production with a concomitant decrease of m ... | 2006 | 16433335 |
| activation of coagulation during hemodialysis: effect of blood lines alone and whole extracorporeal circuit. | activation of coagulation during hemodialysis (hd) is a relevant clinical problem, especially when patients at risk of bleeding are treated. however, little is known about the relative contribution of the various components of the circuit to the thrombotic process. thus, an experimental model was developed that is aimed at evaluating biochemical markers of coagulation activation at different times and sites throughout the hd circuit. a hd blood-tubing set with integrated arterial and venous cham ... | 2006 | 16433843 |
| tumour features in the control and screening arm of a randomized trial of prostate cancer. | to compare tumour characteristics at the time of diagnosis of cancers detected in the screening and control arm at the rotterdam section of the european randomized study of screening for prostate cancer. | 2006 | 16434136 |
| whole genome amplification of plasma-circulating dna enables expanded screening for allelic imbalance in plasma. | apoptotic and necrotic tumor cells release dna into plasma, providing an accessible tumor biomarker. tumor-released plasma-circulating dna can be screened for tumor-specific genetic changes, including mutation, methylation, or allelic imbalance. however, technical problems relating to the quantity and quality of dna collected from plasma hinder downstream genetic screening and reduce biomarker detection sensitivity. here, we present a new methodology, blunt-end ligation-mediated whole genome amp ... | 2006 | 16436631 |
| squamous cell carcinoma of the external auditory canal and middle ear: an operation combined with preoperative chemoradiotherapy and a free surgical margin. | treatment outcomes for squamous cell carcinoma of the temporal bone were evaluated regarding stage, therapeutic strategy, and prognostic factors. | 2006 | 16436996 |
| endocrine abnormalities in anorexia nervosa. | anorexia nervosa (an) is a psychiatric disorder characterized by the classic triad: amenorrhea, weight loss, and behavioral changes. it is generally seen in young, white women under 25 and is particularly common in adolescence. the mortality of the disease varies between 5.1% and 13%. the endocrine changes associated with an have been studied in depth and provide strong evidence for hypothalamic dysfunction. all are secondary and reverse with weight gain. in general, gonadotropin (fsh, lh) level ... | 2003 | 16437012 |
| [prognostic factor of primary liver cancer treated by hypofractionated three-dimensional conformal radiotherapy]. | to evaluate the toxicity and efficacy of primary liver cancer (plc) treated by hypofractionated three-dimensional conformal radiotherapy (3dcrt) and investigate the prognostic factors. | 2005 | 16438872 |
| dda helicase unwinds a dna-pna chimeric substrate: evidence for an inchworm mechanism. | helicases are ubiquitous enzymes involved in all aspects of dna metabolism including replication, repair, recombination, and transcription. the mechanism of the bacteriophage t4 dda helicase was investigated by preparing a dna-pna chimeric substrate. surprisingly, dda was able to unwind a substrate containing 12 pna moieties in the loading strand of the enzyme. we suggest a mechanism whereby the dda helicase contains two distinct dna binding domains which allow an inchworm mechanism for transloc ... | 2006 | 16439125 |
| early cardiac hypertrophy induced by thyroxine is accompanied by an increase in vegf-a expression but not by an increase in capillary density. | cardiac hypertrophy in response to hyperthyroidism is well known. however, the effects on cardiac microcirculation are still controversial in this model. the present study evaluated the effects of acute administration of two different thyroxine (t4) dose levels on the angiogenic response in the myocardium. capillary density (cd), the cd to fiber density (fd) ratio (cd/fd), and intercapillary distance (icd) were assessed, as was ventricle weight (vw) to body weight (bw) ratio (vw/bw). collagen i ... | 2006 | 16440199 |
| [preclinical diagnosis of traumatic paraplegia or tetraplegia -- a prospective study in 100 patients]. | to avoid neurological deterioration, traumatic spinal cord injury has to be recognized as early as possible. aim of the study was to analyze alterations of the neurological level of injury in the preclinical interval. | 2006 | 16440253 |
| usefulness of endoscopic ultrasonography in preoperative tnm staging of gastric cancer. | to evaluate the value of endoscopic ultrasonography (eus) in the preoperative tnm staging of gastric cancer. | 2006 | 16440415 |
| [the practice guideline 'carcinoma of the oral cavity and oropharynx']. | with a total incidence of ca. 800 per year, oral cavity or oropharyngeal carcinoma represents only a small fraction of the total number of cases of cancer in the netherlands. in order to achieve as much nationwide uniformity as possible in its diagnosis, treatment, after-care and follow-up, a practice guideline has now been developed. the diagnosis of the primary tumour and the neck, careful clinical examination is complemented by echography, often combined with a biopsy, mri and ct, while techn ... | 2006 | 16440562 |
| evaluation of possible goitrogenic and anti-thyroidal effect of nitrate, a potential environmental pollutant. | nitrate is a wide spread contaminant of ground and surface water. the source of nitrate in the ground water may be from run off or seepage from fertilized soil, municipal or industrial waste water, land fills, septic system, urban drainage or decaying plants. human and animal systems are affected severely on nitrate exposure. the study was to investigate the effect of dietary nitrate exposure on the thyroid status along with the state of iodine nutrition. rats were fed diet containing 3% potassi ... | 2005 | 16440845 |
| taiwanese vegetarians have higher insulin sensitivity than omnivores. | the present study was designed to examine the effects of habitual consumption of taiwanese vegetarian diets on hormonal secretion, and on lipid and glycaemic control. of the ninety-eight healthy female adults recruited from hualien, taiwan (aged 31-45 years), forty-nine were buddhist lactovegetarians and forty-nine were omnivores. dietary intakes were measured, and blood levels of nutrients and hormones were analysed. vegetarians consumed less energy, fat and protein, but more fibre than the omn ... | 2006 | 16441925 |
| reconstruction of large defects postmandibulectomy for oral cancer using plates and myocutaneous flaps: a long-term follow-up. | a series of 28 consecutive cases of mandibular reconstruction by means of reconstructive plates and myocutaneous flaps were reviewed. in all cases mandibular resection was indicated for treatment of squamous cell carcinoma of the oral cavity: 25 pelviglossomandibulectomies (resulting in large defects from the angle of the mandible), 2 commando operations (resulting in lateral defects) and 1 anterior sectional mandibulectomy (resulting in an anterior defect). tumour stages were t1-t2 (4 cases) an ... | 2006 | 16442779 |
| effect of selenium supplementation on thyroid hormone levels and selenoenzyme activities in growing lambs. | the aim of the present study was to evaluate the effects of selenium supplementation on thyroid hormone metabolism and selenoenzyme activities in lambs. twelve 20-d-old male lambs were assigned to one of two diets: a (0.11 ppm se) and b (supplemented with 0.2 ppm selenium as sodium selenite). blood samples were collected weekly for the determination of t3, t4, and selenium levels. the response to thyrotropin-releasing hormone (trh) challenge was estimated at the 11th and 20th wk. animals were sl ... | 2006 | 16444004 |
| congenital hypothyroidism and iodine status in turkey: a comparison between the data obtained from an epidemiological study in school-aged children and neonatal screening for congenital hypothyroidism in turkey. | congenital hypothyroidism and endemic iodine deficiency are the most common cause of mental retardation. however in both cases retardation is of nonpreventable nature. | 2003 | 16444153 |
| thyroid function in pregnancy - maternal-fetal relationship in health and disease. | the article reviews the changes in maternal and fetal thyroid function during pregnancy. during the first trimester the fetus is dependant on maternal thyroxine and later on the direct supply of iodine from the mother for its own thyroxine production. the placental deiodinase metabolism provides the fetus with additional iodine and protects the fetus from excessive iodothyronine transfer and enables the development of its own hypothalamic pituitary thyroid axis. maternal and fetal hypothyroxinem ... | 2003 | 16444155 |
| neonatal thyroid screening: methods-efficiency-failures. | newborn screening for congenital hypothyroidism (ch) is one of the major achievements of preventive medicine, as the condition occurs frequently (1/4000 newborns) and results in brain damage if not detected and treated in the first few days of life. measurement of t4 and/or tsh in dried blood spots collected on the second through fifth days of life are the most widely used methods in screening programs for ch currently. some children with the disease may be missd in any screening program, howeve ... | 2003 | 16444156 |
| congenital hypothyroidism clinical aspects and late consequences. | congenital hypothyroidism (ch) is the commonest treatable cause of mental retardation. the prevelance is 1/3000 - 1/4000 live births worldwide. the importance of ch is that, the longer the diagnosis of ch is delayed, the higher the risk of mental retardation and neurologic sequale; such as poor motor coordination, ataxia, spastic diplegia, muscular hypotonia, strabismus, learning disability and diminished attention span. the most common cause of permenant ch is thyroid dysgenesis (85-90%) in whi ... | 2003 | 16444157 |
| thyroid hormone resistance in children. | thyroid hormone resistance (rth) is characterized by the diminished response of thyroid hormone-responsive tissues in varying degrees in association with elevated serum levels of total and free t4 and t3 and inappropriately normal or elevated tsh levels. in almost all cases it is due to different mutations in only one allele of the thyroid hormone receptor beta gene which blocks the action of normal allele thus producing dominantly inherited rth. in rth, varying degrees of target tissue responsi ... | 2003 | 16444158 |
| [poorly differentiated thyroid carcinomas: new therapeutic considerations]. | for most differentiated thyroid carcinomas, as papillary and follicular carcinomas, following total thyroidectomy and 131i therapy for thyroid remnant ablation, treatment with thyroid hormones to suppress tsh levels will reduce the growth of any remaining thyroid cancer cells, and thyroid cell-specific radiation therapy will either cure or control the disease. thyroid carcinomas are considered poorly differentiated when they start to lose such functions as iodine uptake and thyrotropin-dependenc ... | 2006 | 16444353 |
| randomized phase ii study of neoadjuvant combined-modality chemoradiation for distal rectal cancer: radiation therapy oncology group trial 0012. | to evaluate the rate of pathologic complete response and toxicity of neoadjuvant chemoradiation for advanced t3/t4 distal rectal cancers in a randomized phase ii study | 2006 | 16446336 |
| neoadjuvant capecitabine and oxaliplatin followed by synchronous chemoradiation and total mesorectal excision in magnetic resonance imaging-defined poor-risk rectal cancer. | to evaluate neoadjuvant capecitabine/oxaliplatin before chemoradiotherapy (crt) and total mesorectal excision (tme) in newly diagnosed patients with magnetic resonance imaging (mri) -defined poor-risk rectal cancer. | 2006 | 16446339 |
| endoscopic ultrasonography for gastric cancer: does it influence treatment? | this study aimed to evaluate the utility and shortcomings of endoscopic ultrasound (eus) in tumor node metastasis (tnm) staging of gastric cancer and its influence on treatment. | 2006 | 16446988 |
| charge reduced electrospray size spectrometry of mega- and gigadalton complexes: whole viruses and virus fragments. | the ability to analyze and identify large macromolecular complexes whose molecular weight is beyond the analyzable range of mass spectrometry is of great interest. the size of such complexes makes them suitable for analysis via mobility size spectrometry. in this work, charge reduced electrospray size spectrometry was used for the analysis of bacteriophage viruses with total molecular masses ranging from 3.6 mda up to the gigadalton range. the electrospray source used was operated in "cone jet" ... | 2006 | 16448059 |
| association of circulating antibodies against double-stranded and single-stranded dna with thyroid autoantibodies in graves' disease and hashimoto's thyroiditis patients. | the occurrence of antinuclear antibody (ana), rheumatoid factor (rf), antibodies to double-stranded dna (anti-dsdna) and to single-stranded dna (anti-ssdna) was investigated in 51 patients with autoimmune thyroid diseases (aitd), and in 25 matched control subjects. in comparison with controls, the prevalence of anti-dsdna was 74.5% in aitd patients (p=0.0001), 82.0% in 39 hyperthyroid graves' disease (gd) (p=0.0001), and 50.0% in 12 euthyroid hashimoto's thyroiditis (ht) patients (p=0.0001). the ... | 2006 | 16450315 |
| beta1 integrin inhibitory antibody induces apoptosis of breast cancer cells, inhibits growth, and distinguishes malignant from normal phenotype in three dimensional cultures and in vivo. | current therapeutic approaches to cancer are designed to target molecules that contribute to malignant behavior but leave normal tissues intact. beta(1) integrin is a candidate target well known for mediating cell-extracellular matrix (ecm) interactions that influence diverse cellular functions; its aberrant expression has been implicated in breast cancer progression and resistance to cytotoxic therapy. the addition of beta(1) integrin inhibitory agents to breast cancer cells at a single-cell st ... | 2006 | 16452209 |
| the bacteriophage t4 inhibitor and coactivator asia inhibits escherichia coli rna polymerase more rapidly in the absence of sigma70 region 1.1: evidence that region 1.1 stabilizes the interaction between sigma70 and core. | the n-terminal region (region 1.1) of sigma70, the primary sigma subunit of escherichia coli rna polymerase, is a negatively charged domain that affects the dna binding properties of sigma70 regions 2 and 4. region 1.1 prevents the interaction of free sigma70 with dna and modulates the formation of stable (open) polymerase/promoter complexes at certain promoters. the bacteriophage t4 asia protein is an inhibitor of sigma70-dependent transcription from promoters that require an interaction betwee ... | 2006 | 16452409 |
| colonoscopic findings of symptomatic patients aged 50 to 80 years suggest that work-up of tumour suspicious symptoms hardly reduces cancer-induced mortality. | the risk of colorectal cancer (crc) starts to increase at the age of 50 years in average persons without special risk factors. the significance of clinical symptoms and frequency of endoscopies done at this age are hitherto unknown. we do not know the stage of colorectal cancers nor the distribution of advanced neoplasms in symptomatic persons above 50 years. these data are of interest to validate the necessity of screening programmes, to define the target population and to interpret results of ... | 2005 | 16453208 |
| ligation of endogenous trna 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of chlamydomonas. | trna preparations from chlamydomonas and wheat germ contain small amounts of trna 5' halves and corresponding 3' halves. incubation of cell-free extracts from the two sources with [gamma-p]atp yielded 5'-p-labeled trna 3' halves which were joined to their corresponding 5' counterparts to form mature trna containing 2'-phosphomonoester,3', 5'-phosphodiester bonds. trna 3' halves labelled with t4 kinase were purified, sequenced and also joined to their 5' counterparts. it is proposed that these tr ... | 1983 | 16453445 |
| measurement of total thyroxine concentration in serum from dogs and cats by use of various methods. | to compare results obtained from assay of total thyroxine (t4) concentration in serum of dogs and cats by use of 4 methods. | 2006 | 16454630 |
| primary hydatid disease of the spine: an unusual case. | hydatid disease or hydatidosis caused by echinococcus granulosus is the most widespread, serious human cestode infection in the world. bone involvement is reported in 0.5-4% with spinal involvement reported in 50% of these cases. we report a case of this rare entity of an isolated primary hydatid cyst of the spine in a 15-year-old boy, who presented with progressive back pain for 8 months, lower extremity weakness and urinary incontinence for 3 months. neurological examination was suggestive of ... | 2005 | 16455566 |
| safety of medications and hormones used in the treatment of pediatric thyroid disorders. | levo-thyroxine (l-t4) is used to treat children with any form of hypothyroidism. in fact, l-t4 is the natural hormone and the principal product secreted by the thyroid. it is then converted into t3, the active compound at the tissue level, according to the temporary needs of the organism. therefore, l-t4 can replace thyroid function in hypothyroid patients on a physiological basis. l-t4 administration is a safe and beneficial treatment that can be easily monitored by the concomitant measurement ... | 2004 | 16456491 |
| role of radiotherapy with surgery for t3 and resectable t4 rectal cancer: evidence from randomized trials. | the main treatment for resectable rectal cancer t2-t4 n0-n2 m0 is surgery. the benefit of preoperative or postoperative radiation therapy can be analyzed in terms of improvement of local control, sphincter preservation, and survival weighted against increased toxicity. | 2006 | 16456638 |
| adjuvant systemic therapies in patients with colorectal cancer: an audit on clinical practice in italy. | rarely are conclusions from clinical trials summarized in international consensus conferences and promptly transferred to patient care. the adjuvant therapy for colorectal cancer used in daily clinical practice in italy is described and compared with the recommendations of the 1990 nih consensus conference. | 2005 | 16457144 |
| [t4-type bacteriophages: ubiquitous components of the "dark matter" of the biosphere]. | | 2006 | 16457745 |
| src-3/aib1 protein and gene amplification levels in human esophageal squamous cell carcinomas. | it has been suggested that the steroid receptor coactivatior-3 (src-3) gene, also known as aib1, actr, rac3, p/cip and tram-1, located at 20q12, plays an oncogenic role in several types of human cancers. in this study, we examined the encoded protein expression of src-3 and its copy number in 221 human esophageal squamous cell carcinomas (esccs). in this escc series, the overexpression and increased copy number of src-3 gene was detected in 46 and 13% of esccs, respectively. in addition, overexp ... | 2007 | 16458427 |
| adaptive radiotherapy for invasive bladder cancer: a feasibility study. | to evaluate the feasibility of adaptive radiotherapy (art) in combination with a partial bladder irradiation. | 2006 | 16458776 |
| the predictive value of clinical evaluation of response to neoadjuvant chemoradiation therapy for rectal cancer. | multimodality therapy has become the standard treatment for patients with locally advanced (t3 and t4) rectal carcinoma. accurate preoperative staging of the patients with rectal cancer has increased in importance because the selection of patients with transmural rectal cancer (t3 or t4) or node-positive disease leads to a previous nonsurgical neoadjuvant treatment. the purpose of this study was to evaluate the predictive value of the clinical response to neoadjuvant therapy on the basis of path ... | 2005 | 16459636 |
| increasing illness severity in very low birth weight infants over a 9-year period. | recent reports have documented a leveling-off of survival rates in preterm infants through the 1990's. the objective of this study was to determine temporal changes in illness severity in very low birth weight (vlbw) infants in relationship to the outcomes of death and/or severe ivh. | 2006 | 16460568 |
| biocontrol of the food-borne pathogens listeria monocytogenes and salmonella enterica serovar poona on fresh-cut apples with naturally occurring bacterial and yeast antagonists. | fresh-cut apples contaminated with either listeria monocytogenes or salmonella enterica serovar poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. while most of the antagonists increased the growth of the food-borne pathogens, four of them, including gluconobacter asaii (t1-d1), a candida sp. (t4-e4), discosphaerina fagi (st1-c9), and metschnikowia pulcherrima (t1-e2), proved ef ... | 2006 | 16461659 |
| isolation and characterization of a cyanophage infecting the toxic cyanobacterium microcystis aeruginosa. | we isolated a cyanophage (ma-lmm01) that specifically infects a toxic strain of the bloom-forming cyanobacterium microcystis aeruginosa. transmission electron microscopy showed that the virion is composed of anisometric head and a tail complex consisting of a central tube and a contractile sheath with helical symmetry. the morphological features and the host specificity suggest that ma-lmm01 is a member of the cyanomyovirus group. using semi-one-step growth experiments, the latent period and bur ... | 2006 | 16461672 |
| construction and complementation of in-frame deletions of the essential escherichia coli thymidylate kinase gene. | this work reports the construction of escherichia coli in-frame deletion strains of tmk, which encodes thymidylate kinase, tmk. the tmk gene is located at the third position of a putative five-gene operon at 24.9 min on the e. coli chromosome, which comprises the genes pabc, yceg, tmk, holb, and ycfh. to avoid potential polar effects on downstream genes of the operon, as well as recombination with plasmid-encoded tmk, the tmk gene was replaced by the kanamycin resistance gene kka1, encoding amin ... | 2006 | 16461678 |
| optimization of in vitro transcription and full-length cdna synthesis using the t4 bacteriophage gene 32 protein. | we evaluated the effect of the t4 bacteriophage gene 32 protein (t4gp32) on in vitro transcription and reverse transcription. t4gp32 doubled the yield of in vitro transcripts obtained with t7 rna polymerase and increased the yield of cdna synthesis when used in combination with an rnaseh-deficient moloney murine leukemia virus [au: ok] reverse transcriptase. the positive effect could be correlated with the rna chaperone activity of t4gp32. t4gp32 stimulated the synthesis of long cdnas, particula ... | 2005 | 16461948 |
| evaluation of esophageal peristalsis in patients with esophageal tumors: initial experience with cine mr imaging. | we evaluated esophageal peristalsis in patients with esophageal tumors by cine mr using steady-state free precession (ssfp) sequence and correlated the alteration of the esophageal peristalsis with clinical symptoms and tumor stages. thirteen patients with pathologically proven esophageal tumors, including 12 esophageal cancers and one submucosal leiomyoma, underwent cine mri using true fast imaging with steady precession (truefisp) sequence, which is one ssfp sequence, after contrast-enhanced m ... | 2005 | 16462130 |
| correction of osteoporotic fracture deformities with global sagittal imbalance. | osteoporotic spinal deformities with global sagittal imbalance have devastating effects on patients. however, treatment is difficult and there is a high risk of perioperative medical and mechanical complications. patients with osteoporotic spinal deformities are not ideal candidates for posterior pedicle subtraction osteotomy; the condition necessitates combined anterior-posterior for corrections of the deformity. we ascertained the complication rates and efficacy of the anterior-posterior surge ... | 2006 | 16462430 |
| definitive radiotherapy for squamous cell carcinoma of the base of tongue. | to evaluate definitive radiotherapy (rt) for treatment of base of tongue cancer. | 2006 | 16462500 |
| carcinoma of the buccal mucosa: analysis of clinical presentation, outcome and prognostic factors. | records of 100 consecutive cases of carcinoma of the buccal mucosa treated in our institute between january 2000 and december 2003 were analyzed for clinical presentation, patterns of care, disease-free survival (dfs) and prognostic factors. there were 75 males and 25 females. the mean age was 50 years. ninety five gave a history of abuse of oral tobacco products. only 20 patients were in stage i or ii. sixty one patients had stage iv disease. sixty six patients were treated with radical intent ... | 2006 | 16464632 |
| [endocrinology]. | there is now convincing evidence that the risks of hormone replacement therapy in post-menopausal women exceed its benefits. similarly, it has now been shown that the current use of low-dose oral contraceptives significantly increases the risk for cardiac and arterial thrombotic events. with regard to hypothyroidism, it is now clearly established that the adjunction of t3 to t4 does not offer any benefits. furthermore, a study has now demonstrated that patients with no symptoms of coronary heart ... | 2006 | 16465939 |
| thyroid hormone profile during annual reproductive cycle of diploid and triploid catfish, heteropneustes fossilis (bloch). | triploid fishes generally show sterility along with retarded gonadal development and aneuploid gametes. in teleosts, thyroid hormones influence seasonal adaptations and annual events such as reproduction. in addition, thyroid hormone deposition in matured ova is important for reproductive success as the role of thyroid hormones in early development and metamorphosis is well established. the present study deals with measurements of free and total thyroxine (t4) and triiodothyronine (t3) in the pl ... | 2006 | 16466725 |
| perineural invasion has no prognostic value in patients with invasive breast carcinoma. | no studies examining the relationship between perineural invasion (pni) and clinicopathological factors in invasive breast cancer have been published. therefore, we investigated this association and analyzed the effect of pni on disease-free survival (dfs) in patients with invasive breast carcinoma. pni evaluation was performed on hematoxylin- and eosin-stained tissue sections of surgical specimens from 377 patients. pni was assessed as positive when cancer cells were seen in the perineurium or ... | 2006 | 16466917 |
| three-week thyroxine withdrawal: a thyroid-specific quality of life study. | the purpose of this study is to determine the impact of a 3-week t4 withdrawal test on the quality of life (qol) of patients undergoing investigation for residual/recurrent well-differentiated thyroid cancer. | 2006 | 16467714 |
| [effects of a programme for structured outpatient follow-up care after inpatient rehabilitation of obese children and adolescents--a multicentre, randomized study]. | inpatient rehabilitation is an important contributor to the treatment of child and adolescent obesity with comorbidity in germany. thus far outpatient follow-up care has not been subject to research. the main goal of this study is the development and longer-term evaluation of a programme for structured outpatient follow-up care by primary care physicians after inpatient rehabilitation of obese children and adolescents. | 2006 | 16468112 |
| prognostic value of thrombocytosis in renal cell carcinoma. | we correlated platelet count to renal tumor characteristics, and evaluated the potential prognostic value of thrombocytosis in localized and metastatic tumors. | 2006 | 16469566 |
| renal cell carcinoma with nodal metastases in the absence of distant metastatic disease (clinical stage txn1-2m0): the impact of aggressive surgical resection on patient outcome. | nodal disease in the setting of metastatic renal cell carcinoma is associated with poor prognosis. however, to our knowledge the biology of nodal metastases in the absence of metastatic disease is unknown. we reviewed our experience with treating this subset of patients with aggressive surgical resection. | 2006 | 16469567 |
| comparison of anterior and posterior double-rod instrumentation for thoracic idiopathic scoliosis: results of 141 patients. | ventral derotation spondylodesis, according to zielke, achieves good results in operative treatment of idiopathic thoracic scolioses. corrections of scoliotic major and secondary curve as well as derotation of the spine are reliably performed. the high rate of rod fractures with subsequent correction loss as well as a proportionate kyphogenic effect represents a problem. by keeping to the correcting principle, anterior double-rod instrumentation (halm-zielke instrumentation) is to be stable in a ... | 2006 | 16470398 |
| the gross anatomy of the extrathoracic course of the intercostobrachial nerve. | recent reports emphasize the importance of preserving the intercostobrachial nerve (icbn) during surgical procedures (i.e., mastectomy, axillary clearance). however, a limited number of scientific reports explore the surgical anatomy of this nerve. we dissected 100 adult human formalin-fixed cadavers (200 axillae). in all the cadavers the icbn was present with variant contributions from intercostal nerves t1, t2, t3, and t4. the arrangements of the icbn were typed as i through viii. the componen ... | 2006 | 16470542 |
| supracricoid laryngectomy with cricohyoidoepiglottopexy for advanced glottic cancer. | supracricoid laryngectomy with cri-cohyoidoepiglottopexy (chep) is a conservative surgical procedure indicated in selected cases of advanced glottic carcinoma. | 2006 | 16470876 |
| [the sentinel node in cancer of the oral cavity, pharynx and larynx]. | the evaluation of the importance of the prognostic of sentinel node biopsy in head and neck cancer with n0 neck. | 2005 | 16471184 |
| influence of micrometastasis on n stage in gastric cancer and clinical application. | in order to evaluate the real extent of lymph node metastasis (lnm) in gastric cancer, an immunohistochemical (ihc) analysis was performed. we examined 11173 lymph nodes removed from 355 patients with all stages of gastric carcinoma. tissue preparations were stained with cytokeratin 18, monoclonal antibody against cytokeratin. micrometastases were found in 2.5% of the lymph nodes and in 31.3% of patients. the incidence of the patients with lnm increased to 9.1% in t(1m) (n = 99), 31.6% in t(1sm) ... | 2005 | 16471315 |
| feasibility of preoperative chemoradiation in rectal cancer patients aged 70 and older. | in the present study, we report results of 28 rectal cancer patients, aged 70 years and older, treated with preoperative radiotherapy and 5fu concomitant chemotherapy. twenty-eight out of 136 patients treated in our department between 1997 and 2004 aged > or = 70 years, mean 73 (range 70-81); 3 t2, 18 t3, 7 t4; 15 n0, 5n1, 8 n2; radiotherapy (5040 cgy, 28 fractions) was delivered combined with 5fu - based concomitant chemotherapy. compliance to chemoradiotherapy was excellent. major acute toxici ... | 2005 | 16471316 |
| expression of highly toxic genes in e. coli: special strategies and genetic tools. | escherichia coli (e. coli) remains the most efficient widely-used host for recombinant protein production. well-known genetics, high transformation efficiency, cultivation simplicity, rapidity and inexpensiveness are the main factors that contribute to the selection of this host. with the advent of the post-genomic era has come the need to express in this bacterium a growing number of genes originating from different organisms. unfortunately, many of these genes severely interfere with the survi ... | 2006 | 16472168 |
| effect of chlorpyrifos-methyl on steroid and thyroid hormones in rat f0- and f1-generations. | chlorpyrifos-methyl (cpm) suppressed androgenic activity in hershberger assay using castrated rats. acute oral lowest-observed-adverse-effect-level (loael) and no-observed-adverse-effect-level (noael) was evaluated as 12 and 0.1 mg/kg bw, respectively, based on its major effect of cholinesterase inhibition. also, repeated oral noael was 0.1 mg/kg bw/day based on adrenal damage in rats. we investigated one-generation reproductive toxicity of cpm focusing on endocrine-disrupting effects by the adm ... | 2006 | 16472551 |
| antioxidant status and oxidative stress in professional rugby players: evolution throughout a season. | physical training is known to increase the antioxidant defence system and reduce exercise-induced oxidative stress. however, intense physical aerobic and anaerobic training and competition such as those imposed on professional rugby players, can induce an increase of oxidative stress which can be implicated with the arrival of overtraining. the aim of this study was to test the effect of training and competition load on oxidative stress, antioxidant status, haematological, and cell damage marker ... | 2006 | 16475052 |
| expression of cancer testis antigens in head and neck squamous cell carcinomas. | there is considerable interest in the expression of cancer testis (ct) antigens in human cancers, because they may serve as the basis for diagnostic tests or an immunologic approach to therapy, or as prognostic markers. | 2006 | 16475205 |