| [changes in oxygen metabolism in hepatitis b gravis in peri-operative stages of orthotopic liver transplantation]. | to investigate the changes in oxygen metabolism in peri-operative stages in hepatitis b gravis and non-hepatitis b gravis undergoing orthotopic liver transplantation (olt). | 2005 | 16259915 |
| its1 sequence variabilities correlate with 18s rdna sequence types in the genus acanthamoeba (protozoa: amoebozoa). | the subgenus classification of the ubiquitously spread and potentially pathogenic acanthamoebae still poses a great challenge. fifteen 18s rdna sequence types (t1-t15) have been established, but the vast majority of isolates fall into sequence type t4, and so far, there is no means to reliably differentiate within t4. in this study, the first internal transcribed spacer (its1), a more variable region than the 18s rrna gene, was sequenced, and the sequences of 15 different acanthamoeba isolates w ... | 2006 | 16261355 |
| environmental signals: synthetic humic substances act as xeno-estrogen and affect the thyroid system of xenopus laevis. | according to outdated paradigms humic substances (hs) are considered to be refractory or inert that do not directly interact with aquatic organisms. however, they are taken up and induce biotransformation activities and may act as hormone-like substances. in the present study, we tested whether hs can interfere with endocrine regulation in the amphibian xenopus laevis. in order to exclude contamination with phyto-hormones, which may occur in environmental isolates, the artificial hs1500 was appl ... | 2005 | 16263388 |
| thyroid-hormone-disrupting chemicals: evidence for dose-dependent additivity or synergism. | endocrine disruption from environmental contaminants has been linked to a broad spectrum of adverse outcomes. one concern about endocrine-disrupting xenobiotics is the potential for additive or synergistic (i.e., greater-than-additive) effects of mixtures. a short-term dosing model to examine the effects of environmental mixtures on thyroid homeostasis has been developed. prototypic thyroid-disrupting chemicals (tdcs) such as dioxins, polychlorinated biphenyls (pcbs), and polybrominated diphenyl ... | 2005 | 16263510 |
| in utero exposure to dioxins and polychlorinated biphenyls and its relations to thyroid function and growth hormone in newborns. | the aim of this study is to examine the association between transplacental exposure to dioxins/polychlorinated biphenyls (pcbs) and thyroid and growth hormones in newborns. we recruited 118 pregnant women, between 25 and 34 years of age, at the obstetric clinic. personal data collected included reproductive and medical histories and physical factors. clinicians gathered placental and umbilical cord serum upon delivery and carefully scored the 118 newborns, making both structural and functional a ... | 2005 | 16263525 |
| molecular architecture and ligand recognition determinants for t4 rna ligase. | rna ligase type 1 from bacteriophage t4 (rnl1) is involved in countering a host defense mechanism by repairing 5'-po4 and 3'-oh groups in trna(lys). rnl1 is widely used as a reagent in molecular biology. although many structures for dna ligases are available, only fragments of rna ligases such as rnl2 are known. we report the first crystal structure of a complete rna ligase, rnl1, in complex with adenosine 5'-(alpha,beta-methylenetriphosphate) (ampcpp). the n-terminal domain is related to the eq ... | 2006 | 16263720 |
| neuropsychological function and symptoms in subjects with subclinical hypothyroidism and the effect of thyroxine treatment. | our objective was to examine the relation between neuropsychological function and subclinical hypothyroidism (sht), defined as serum tsh of 3.5-10.0 miu/liter and normal serum free t4 and free t3 levels, and to study the effect of t4 supplementation. | 2006 | 16263815 |
| survival and death causes in differentiated thyroid carcinoma. | survival studies in differentiated thyroid carcinoma (dtc) may be biased because they have been performed in heterogeneous populations. in addition, specific death causes in dtc have not been documented well in the literature. | 2006 | 16263822 |
| osteopontin gene expression in the aorta and the heart of propylthiouracil-induced hypothyroid mice. | it is known that there is abnormal osteopontin (opn) expression at the sites of atherosclerotic lesions. in the apolipoprotein e gene knockout (apoe-ko) mouse, a model of the atherosclerotic process, altered cholesterol metabolism with associated increase in opn expression is evident at 12-22 weeks in the aorta and at 22 weeks in the heart. in this study, we analyzed another animal model of hypothyroid mice created by ingestion of propylthiouracil (ptu). after 2 weeks of ptu ingestion, the anima ... | 2005 | 16265595 |
| [future organisation of colorectal cancer surgery in denmark]. | we recommend that in the future, surgery for colorectal cancer (crc) in denmark should be done in 10 to 15 colorectal units with an uptake zone of 350,000-500,000 citizens each. these units should perform both acute and elective crc surgery and acute surgical treatment of other intestinal diseases. in each unit, a senior colorectal surgeon should be available on a 24-hour shift, and there should be sufficient diagnostic and theatre capacity to ensure optimal treatment levels. a stoma clinic shou ... | 2005 | 16266578 |
| the role of door orientation on occupant injury in a nearside impact: a ciren, madymo modeling and experimental study. | this study addressed the effects of vehicle height mismatch in side impact crashes. a light truck or suv tends to strike the door of a passenger car higher causing the upper border to lead into the occupant space. conversely, an impact centered lower on the door, from a passenger car, causes the lower border to lead. we proposed the hypothesis that the type of injury sustained by the occupant could be related to door orientation during its intrusion into the passenger compartment. | 2005 | 16266946 |
| [should we detect osteoporosis, and how?]. | osteoporotic vertebral and hip fractures, which are very frequent among elderly, are associated to an excess of mortality, as well as to an important morbidity. in switzerland, annual costs of in-patients' treatments of osteoporosis are beyond chf 700 millions. as several effective treatments against osteoporosis are available, a systematic screening is justified. the methodical search for risk factors of fracture and the recognized medical indications for bone densitometry are of prime necessit ... | 2005 | 16268446 |
| cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation. | formation of a water-expelling nonpolar core is the paradigm of protein folding and stability. although experiment largely confirms this picture, water buried in "hydrophobic" cavities is required for the function of some proteins. hydration of the protein core has also been suggested as the mechanism of pressure-induced unfolding. we therefore are led to ask whether even the most nonpolar protein core is truly hydrophobic (i.e., water-repelling). to answer this question we probed the hydration ... | 2005 | 16269539 |
| umbilical cord blood tsh levels in term neonates: a screening tool for congenital hypothyroidism. | this study was conducted to find normative values for thyroid stimulating hormone (tsh) in 1200 cord blood samples of term babies whose mothers were not on any thyroid medications. tsh was estimated within 24 hrs by enzyme immunoassay. a full thyroid profile, viz, t3, t4, tsh, ft3 and ft4 was done at 7-10 days of age in all babies with cord tsh >20 miu/l. the mean, median and standard deviation for the tsh values for the cohort were 6.13 miu/l, 5.8 miu/l and 4.523 respectively. 22 babies with ts ... | 2005 | 16269841 |
| rate of recalls in congenital hypothyroidism based upon a regional survey in isfahan, iran, using serum t4 and tsh analyses: comparison of two different recall methods. | to evaluate and compare the recall rate in congenital hypothyroidism screening project in isfahan, first using an approach involving measures of both tsh and t4 and then using tsh alone. | 2005 | 16269871 |
| preoperative chemotherapy in children with high-risk medulloblastomas: a feasibility study. | the authors set out to evaluate the feasibility and effectiveness of preoperative chemotherapy in treating high-risk medulloblastomas. | 2005 | 16270682 |
| [expression of adrenomedullin in the tissue with laryngeal carcinoma]. | to observe the expression of adrenomedullin (am) in the patients with laryngeal carcinoma. | 2005 | 16270874 |
| [treatment and survival analysis of pyriform sinus cancer]. | to evaluate the efficacy of surgery for the patients with pyriform sinus carcinoma and analyze the prognostic factors related to the cancer. | 2005 | 16270875 |
| functional role of the n-terminal domain of bacteriophage t4 gene product 11. | bacteriophage t4 late gene product 11 (gp11), the three-dimensional structure of which has been solved by us to 2.0 a resolution, is a part of the virus' baseplate. the gp11 polypeptide chain consists of 219 amino acid residues and the functionally active protein is a three-domain homotrimer. in this work, we have studied the role of gp11 n-terminal domain in the formation of a functionally active trimer. deletion variants of gp11 and monoclonal antibodies recognizing the native conformation of ... | 2005 | 16271026 |
| correlation between mr imaging-derived nasopharyngeal carcinoma tumor volume and tnm system. | to measure nasopharyngeal carcinoma tumor volume based on magnetic resonance images using a validated semiautomated measurement methodology and correlate tumor volume with tnm t classification. | 2006 | 16271442 |
| large-scale systematic study on stability of the ds element and timing of transposition in rice. | activator/dissociation (ac/ds) transposon mutagenesis is a widely used tool for gene identification; however, several reports on silencing of the ac/ds element in starter lines and in stable transposants question the applicability of such an approach in later generations. we have performed a systematic analysis on various aspects of the silencing phenomenon in rice (oryza sativa ssp. japonica cv. nipponbare). high somatic and germinal transposition frequencies observed in earlier generations wer ... | 2006 | 16275658 |
| a randomized phase iii trial of post-operative adjuvant oral fluoropyrimidine versus sequential paclitaxel/oral fluoropyrimidine; and uft versus s1 for t3/t4 gastric carcinoma: the stomach cancer adjuvant multi-institutional trial group (samit) trial. | paclitaxel, s1 and their combined sequential administration is proposed to be examined installing uft as an active control of adjuvant chemotherapy for curatively resected t3-4 gastric cancer in a multicenter phase iii trial. the primary endpoint is disease-free survival and the secondary endpoints are incidence of adverse events, overall survival and compliance. the sample size is 370 per treatment arm (1480 in total) for two hypotheses of the superiority of sequential use of paclitaxel followe ... | 2005 | 16275676 |
| preoperative chemotherapy and radiation therapy for squamous cell carcinoma of the maxillary sinus. | this study was undertaken to assess the prognostic factors for the management of squamous cell carcinoma (scc) of the maxillary sinus, who received preoperative chemotherapy and radiation therapy (rt). we also elucidated the appropriate sequence of chemotherapy. | 2005 | 16275677 |
| seroreactivity to a-type retrovirus proteins in a subset of cats with hyperthyroidism. | the thyroid gland is afflicted in several endocrine, autoimmune, and malignant diseases. previous studies detected immunoreactivity against proteins of a human intracisternal a-type retroviral particle type-i (hiap-i) in serum samples from the majority of patients with graves' disease, an autoimmune disease of the thyroid that can also affect other organs, most prominently the eyes. to determine whether hyperthyroid animals might provide a model for the retroviral involvement in thyroid autoimmu ... | 2005 | 16276511 |
| evaluation of right and left ventricular function using pulsed-wave tissue doppler echocardiography in patients with subclinical hypothyroidism. | previous studies showed that subclinical hypothyroidism (sh) was associated with cardiovascular disorders, such as endothelial dysfunction, atherosclerosis and myocardial dysfunction. only one study investigated left ventricular (lv) function using pulsed tissue doppler echocardiography (tde) in patients with sh. however, no study has used this technique in the identification of right ventricular (rv) function in these patients. we aimed to investigate the effect of sh on rv and lv function usin ... | 2005 | 16277166 |
| prevalence of goiter among schoolchildren from gorgan, iran, a decade after national iodine supplementation: association with age, gender, and thyroperoxidase antibodies. | one decade after universal salt iodization in iran, goiter prevalence, urinary iodine concentration (uic) and thyroperoxidase antibody (tpoab) values were assessed among schoolchildren in gorgan, iran. | 2005 | 16277169 |
| synthesis of 2'-c-difluoromethylribonucleosides and their enzymatic incorporation into oligonucleotides. | [chemical reaction: see text] nucleosides bearing a branched ribose have significant promise as therapeutic agents and biotechnological and biochemical tools. here we describe synthetic entry into a new subclass of these analogues, 2'-c-beta-difluoromethylribonucleosides. we constructed the glycosylating agent 4 in three steps from 1,3,5-tri-o-benzoyl-alpha-d-ribofuranose 1. the key steps included nucleophilic addition of difluoromethyl phenyl sulfone to 2-ketoribose 2 followed by mild and effic ... | 2005 | 16277309 |
| topography of eeg complexity in human neonates: effect of the postmenstrual age and the sleep state. | the topography of the eeg of human neonates is studied in terms of its power spectral density and its estimated complexity as a function of both the postmenstrual age (pma) and the sleep state. the monopolar eegs of three groups of seven neonates (preterm, term and older term) were recorded during active (as) and quiet sleep (qs) from electrodes fp1, fp2, t3, t4, c3, c4, o1 and o2. the existence of changes between groups and sleep states in the power of delta, theta, alpha and beta bands and in ... | 2006 | 16278043 |
| thyroid hormone dysregulation in intrauterine growth retardation associated with maternal malnutrition and/or anemia. | data on the effect of maternal malnutrition and/or anemia on thyroid hormone regulation in human fetuses are scarce, and would be of great importance in examining the relevance of barker's hypothesis, which proposes adaptation of fetuses to undernutrition leading to permanent metabolic and endocrine changes that form the basis of adult diseases. to examine the quantitative variations in thyroid hormone profile of neonates born to malnourished and/or anemic mothers, we quantitated the t3, t4, rt3 ... | 2005 | 16278787 |
| factors influencing variation in basal metabolic rate include fat-free mass, fat mass, age, and circulating thyroxine but not sex, circulating leptin, or triiodothyronine. | basal metabolic rate (bmr) is the largest component of daily energy demand in western societies. previous studies indicated that bmr is highly variable, but the cause of this variation is disputed. all studies agree that variation in fat-free mass (ffm) plays a major role, but effects of fat mass (fm), age, sex, and the hormones leptin, triiodothyrionine (t3), and thyroxine (t4) remain uncertain. | 2005 | 16280423 |
| cystoprostatectomy for effective palliation of symptomatic bladder invasion by prostate cancer. | we evaluated the palliative effect of cystoprostatectomy (cp) for palliating pelvic symptoms in patients with bladder invasion by prostate cancer. | 2005 | 16280761 |
| anti-thyroid methimazole in an acidosis-induced retinopathy rat model of retinopathy of prematurity. | methimazole (mmi), an anti-thyroid drug known to reduce serum levels of l-thyroxine (t4) and insulin-like growth factor-1 (igf-1), has been previously reported to increase the incidence of neovascularization (nv) in an oxygen-induced retinopathy (oir) model of retinopathy of prematurity (rop) in rats. we investigated the effect of mmi on the incidence and severity of nv in a non-oxygen-induced model of rop, acidosis-induced retinopathy (air). | 2005 | 16280976 |
| [changes of the thyroid hormone status in children with cerebral palsy and other neurological pathology]. | content of thyroid hormones (t3 and t4) and thyrotropin (ttg) hormone in blood plasma has been studied in 142 children with neurological pathology (cerebral palsy, perinatal cns lesion). in most cases, there was a significant increase of t3 and t4 and reduction of ttg as compared to control group of healthy children. the changes of thyroid hormones content depended on the patient's age and severity of a pathological condition. a mother pregnancy course impacted on the hormones level. the content ... | 2005 | 16281373 |
| bexarotene--an alternative therapy for progressive cutaneous t-cell lymphoma? first experiences. | a standard therapy for advanced cutaneous t-cell lymphomas has not yet been defined. bexarotene is a new retinoid x receptor-specific retinoid that has been approved for systemic second-line therapy for cutaneous t-cell lymphomas in the usa and europe. in order to evaluate the efficacy of bexarotene in cutaneous t-cell lymphomas, a pilot trial was initiated. | 2003 | 16281814 |
| capecitabine in combination with preoperative radiation therapy in locally advanced, resectable, rectal cancer: a multicentric phase ii study. | the aim of the study was to evaluate tolerance and efficacy of preoperative treatment with capecitabine in combination with radiation therapy (rt) in patients with locally advanced, resectable, rectal cancer. | 2006 | 16282246 |
| opposite roles of the dmd gene in the control of rnase e and rnase ls activities. | when the dmd gene of bacteriophage t4 is defective, expression of middle genes starts normally but drops abruptly. however, the residual expression of middle genes at late stages continues at a higher rate in cells infected with a dmd mutant than with the wild type. in order to understand the complex effects of the dmd gene, we followed changes in the quantity of mrna from a middle gene, uvsy. the uvsy mrna was degraded rapidly by rnase ls at middle stages but stabilized at late stages, suggesti ... | 2005 | 16284417 |
| ratio of serum free triiodothyronine to free thyroxine in graves' hyperthyroidism and thyrotoxicosis caused by painless thyroiditis. | the serum t3 to t4 ratio is a useful indicator for differentiating destruction-induced thyrotoxicosis from graves' thyrotoxicosis. however, the usefulness of the serum free t3 (ft3) to free t4 (ft4) ratio is controversial. we therefore systematically evaluated the usefulness of this ratio, based on measurements made using two widely available commercial kits in two hospitals. eighty-two untreated patients with thyrotoxicosis (48 patients with graves' disease and 34 patients with painless thyroid ... | 2005 | 16284430 |
| pedicle screw fixation of the thoracic spine: an in vitro biomechanical study on different configurations. | an in vitro biomechanical study of different pedicle screw configuration usage on the thoracic spine using a cadaveric model. | 2005 | 16284591 |
| the validity of lenke criteria for defining structural proximal thoracic curves in patients with adolescent idiopathic scoliosis. | retrospective series. | 2005 | 16284594 |
| [xeroderma pigmentosum: children of the moon]. | xeroderma pigmentosum is based on a genetic defect in the dna repair system, which is diagnosed in early childhood. xeroderma pigmentosum is a rare disorder, which is transmitted in an autosomal recessive manner. children with xeroderma pigmentosum display hypersensitivity to ultraviolet (uv) radiation. these patients experience serious sunburns with minimal exposure and then develop poikiloderma in the sun-exposed areas. squamous cell carcinomas, basal cell carcinomas and malignant melanomas al ... | 2003 | 16285494 |
| [concurrent chemoradiotherapy for advanced hypopharyngeal cancer]. | chemotherapy has been shown to be most effective when delivered concurrently with radiation for the patients with advanced stage tumors. we conducted a concurrent chemoradiation using systemic infusion of cisplatin (cddp) and 5-fluorouracil (5-fu). | 2005 | 16285613 |
| a case control study on psychiatric disorders in hashimoto disease and euthyroid goitre: not only depressive but also anxiety disorders are associated with thyroid autoimmunity. | to evaluate the association between mood and anxiety disorders in hashimoto disease and euthyroid goitre in a case control study. | 2005 | 16285879 |
| electrophilic activation of lactams with tf2o and pyridine: expedient synthesis of (+/-)-tetraponerine t4. | [reaction: see text] the electrophilic activation of lactams with triflic anhydride in the presence of pyridine was investigated by nmr. it was found that 2-pyrrolidone led to the clean formation of the corresponding pyridinium imidate in 89% isolated yield. the subsequent nucleophilic addition of organometallic reagents led to 2-substituted dihydropyridines. a synthesis of (+/-)-tetraponerine t4 with three simple building blocks was accomplished in 3 steps (38% yield). | 2005 | 16288516 |
| evaluation of gene expression endpoints in the context of a xenopus laevis metamorphosis-based bioassay to detect thyroid hormone disruptors. | thyroid hormones (th) are important in growth, development and the maintenance of proper cellular metabolism in vertebrates. amphibian metamorphosis is completely dependent on th and forms the basis of a screen for thyroid axis disrupting chemicals that currently relies on external morphological endpoints and changes in thyroid gland histology. the requirement for th-dependent gene expression makes it possible to augment this screen through the addition of molecular endpoints. in order to do thi ... | 2006 | 16289343 |
| re-irradiation of nasopharyngeal carcinoma with intensity-modulated radiotherapy. | to evaluate the treatment outcome in patients with locally recurrent nasopharyngeal carcinoma (npc) treated with intensity-modulated radiation therapy (imrt). | 2005 | 16289398 |
| compaction of dna by gemini surfactants: effects of surfactant architecture. | the interaction between bacteriophage t4 dna and cationic gemini surfactants was studied by the use of fluorescence microscopy. upon addition of surfactant, dna undergoes a transition from random coil to globule, with an intermediate coexistence region. the state behavior of a dna-gemini surfactant system was found to depend on spacer length, valency, head group size, and tail length. a series of alkanediyl-alpha,omega-bis-(dimethylalkylammonium bromide) surfactants with fixed tail length and va ... | 2002 | 16290792 |
| concentration effects on adsorption of bacteriophage t4 lysozyme stability variants to silica. | the adsorption kinetics and dodeceyltrimethylammonium bromide-mediated elution of the wild type and two structural stability mutants of bacteriophage t4 lysozyme were recorded in situ, at silica surfaces. experiments were performed at different solution concentrations, ranging from 0.01 to 1.0 mg/ml. plateau values of adsorbed mass generally increased with increasing solution concentration, with the adsorbed layer being only partially eluted by buffer. treatment with surfactant removed more of t ... | 2002 | 16290814 |
| vancomycin stress response in a sensitive and a tolerant strain of streptococcus pneumoniae. | the vancomycin stress response was studied in streptococcus pneumoniae strains t4 (tigr4) and tupelo. vancomycin affected the expression of 175 genes, including genes encoding transport functions and enzymes involved in aminosugar metabolism. the two-component systems tcs03, tcs11, and ciarh also responded to antibiotic treatment. we hypothesize that the three regulons are an important part of the bacterium's response to vancomycin stress. | 2005 | 16291696 |
| preoperative chemoradiation for rectal cancer causes prolonged pudendal nerve terminal motor latency. | a worsened anorectal function after chemoradiation for high-risk rectal cancer is often attributed to radiation damage of the anorectum and pelvic floor. its impact on pudendal nerve function is unclear. this prospective study evaluated the short-term effect of preoperative combined chemoradiation on anorectal physiologic and pudendal nerve function. | 2006 | 16292664 |
| possible involvement of organic anion transporting polypeptide 1c1 in the photoperiodic response of gonads in birds. | the photoperiodic response of the gonads requires t3, which is generated photoperiodically from t4 by type 2 iodothyronine deiodinase in the hypothalamus. although thyroid hormones were long thought to traverse the plasma membrane by passive diffusion due to their lipophilic nature, it is now known that several organic anion transporting polypeptides (oatp) transport thyroid hormones into target cells. in this study, we have used database searches to isolate dna sequences encoding members of the ... | 2006 | 16293658 |
| thermodynamics of an incommensurate quantum crystal. | we present a theory of the thermodynamics of an incommensurate quantum solid. the ground state of the solid is assumed to be an incommensurate crystal, with quantum zero-point vacancies and interstitials and thus a non-integer number of atoms per unit cell. we show that at low temperature t, the variation of the net vacancy concentration should be as t4 and that the first correction to the specific heat due to this varies as t7; these are quite consistent with experiments on solid helium-4. we a ... | 2005 | 16293755 |
| effects of in utero and lactational exposure to bisphenol a on thyroid status in f1 rat offspring. | bisphenol a (bpa), a xenoestrogen, has been reported to mimic the actions of estrogen or to affect the endocrine glands in vivo and in vitro. in this study, we examined whether in utero and lactational exposure to bpa alters thyroid status in rat f1 offspring. dams were orally administered various doses of bpa (0, 4 or 40 mg/kg body weight per day) from gestation day (gd) 6 through postnatal day (pnd) 20. the bpa and control groups did not differ significantly with respect to plasma thyroxine (t ... | 2005 | 16294924 |
| pathogenic acanthamoeba strains from water sources in jamaica, west indies. | in 2004, samples of tap water and of river and sea water associated with human activities were collected in jamaica, west indies, and checked for free-living acanthamoeba. the morphologies of the cysts and trophozoites observed and the results of pcr-based amplifications with a genus-specific primer pair were used to identify the acanthamoeba isolates. the potential of each isolate as a human pathogen was then evaluated using thermotolerance and osmotolerance assays and two pcr-based assays for ... | 2005 | 16297288 |
| combined radiotherapy, 5-fluorouracil continuous infusion and weekly oxaliplatin in advanced rectal cancer: a phase i study. | the aim of this study was to determine the maximum-tolerated dose (mtd) of weekly oxaliplatin combined with 5-fluorouracil (5fu) continuous infusion administered concomitantly with fractionated radiotherapy in patients presenting advanced rectal cancer. forty-three patients with rectal cancer (stage t3/t4 (n = 24), metastatic (n = 17) and 2 with local recurrence), were included. the radiotherapy dose delivered was 45 gy over 5 weeks (1.8 gy/fraction/day, 5 days per week). the initial weekly oxal ... | 2005 | 16297614 |
| effect of substituting allogenic blood transfusion with autologous blood transfusion on outcomes after radical oesophagectomy for cancer. | the oncologic benefit of avoiding allogenic blood transfusion in oesophageal cancer resection has not been studied. | 2005 | 16299455 |
| l-thyroxine responsive cricopharyngeal achalasia associated with hypothyroidism in a dog. | an eight-year-old, male boxer dog was presented with a one-month history of hindlimb weakness followed by compulsive ineffective drinking, dysphagia, regurgitation and nasal reflux during drinking. a neurological examination revealed weakness and conscious proprioception deficits in both hindlimbs with normal spinal reflexes. the dog's swallowing function was examined by fluoroscopy. this showed normal prehension of the barium paste, bolus formation and contraction of the pharyngeal muscle, but ... | 2005 | 16300118 |
| mechanism of the phosphatase component of clostridium thermocellum polynucleotide kinase-phosphatase. | polynucleotide kinase-phosphatase (pnkp) from clostridium thermocellum catalyzes atp-dependent phosphorylation of 5'-oh termini of dna or rna polynucleotides and ni(2+)/mn(2+)-dependent dephosphorylation of 2',3' cyclic phosphate, 2'-phosphate, and 3'-phosphate ribonucleotides. cthpnkp is an 870-amino-acid polypeptide composed of three domains: an n-terminal module similar to bacteriophage t4 polynucleotide kinase, a central module that resembles the dinuclear metallo-phosphoesterase superfamily ... | 2006 | 16301605 |
| template-independent ligation of single-stranded dna by t4 dna ligase. | t4 dna ligase is one of the workhorses of molecular biology and used in various biotechnological applications. here we report that this ligase, unlike escherichia coli dna ligase, taq dna ligase and ampligase, is able to join the ends of single-stranded dna in the absence of any duplex dna structure at the ligation site. such nontemplated ligation of dna oligomers catalyzed by t4 dna ligase occurs with a very low yield, as assessed by quantitative competitive pcr, between 10(-6) and 10(-4) at ol ... | 2005 | 16302964 |
| survivin expression and its relationship with apoptosis and prognosis in nasal and paranasal sinus carcinomas. | the inverse correlation found in this study between survivin expression and the level of spontaneous apoptosis in nasal and paranasal sinus carcinoma (npsc) suggests that survivin may play an important role in the tumorigenesis of npsc. survivin expression may provide a novel predictive indicator for disease-free survival after surgical resection and postoperative radiochemotherapy in npsc. | 2005 | 16303685 |
| comparison of antihypertensives after coronary artery surgery. | hypertension following coronary artery bypass grafting is a common problem that may result in postoperative myocardial infraction or bleeding, hemodynamic effects were compared in 45 hypertensive coronary bypass patients randomized to receive either diltiazem, nitroglycerin, or sodium nitroprusside. diltiazem was administered as an intravenous bolus of 0.3 mg.kg-1 within 5 min, followed by infusion of 0.1-0.8 mg.kg-1.h-1 in group 1. nitroglycerin was infused at a rate of 1-3 microg.kg.h-1 in gro ... | 2005 | 16304214 |
| the loss of the chloride channel, clc-5, delays apical iodide efflux and induces a euthyroid goiter in the mouse thyroid gland. | genetic inactivation of clc-5, a voltage-gated chloride channel prominently expressed in the kidney, leads to proteinuria because of defective apical endocytosis in proximal tubular cells. because thyroid hormone secretion depends on apical endocytosis of thyroglobulin (tg), we investigated whether clc-5 is expressed in the thyroid and affects its function, using clcn5-deficient knockout (ko) mice. we found that clc-5 is highly expressed in wild-type mouse thyroid ( approximately 40% of mrna kid ... | 2006 | 16306076 |
| effects of rexinoids on thyrotrope function and the hypothalamic-pituitary-thyroid axis. | retinoid x receptor (rxr)-selective retinoids (rexinoids) can cause central hypothyroidism in humans, and this effect has been confirmed in rodent models. in this report, we characterized the effect of rexinoids on the hypothalamic-pituitary-thyroid axis in mice and tsh regulation in a thyrotrope-derived cell line. the synthetic rexinoid (lg 268) suppressed tsh and t4 levels in mice. hypothalamic trh mrna was unaffected, but steady-state pituitary tshbeta mrna levels were significantly lowered, ... | 2006 | 16306084 |
| influence of matrix metalloproteinase 9 (mmp-9) on the metastatic behavior of oropharyngeal cancer. | the role of the single matrix metalloproteinases (mmps) in the metastatic process of squamous cell carcinomas (scc) is still obscure. | 2005 | 16309207 |
| purkinje cell and cerebellar effects following developmental exposure to pcbs and/or mehg. | we recently reported that rats exposed to pcbs and mehg during development were impaired on the rotating rod, a test of balance and coordination that is often indicative of cerebellar damage. in addition, developmental pcb exposure is known to dramatically reduce circulating thyroid hormone concentrations, which may have a negative impact on cerebellar development. therefore, we investigated the effects of combined pcb and mehg exposure on purkinje cells and the cerebellum. the serum and brains ... | 2006 | 16309888 |
| rapid modulation of trh-like peptides in rat brain by thyroid hormones. | recent identification of membrane receptors for t4, t3, 3,5-t2, and 3-iodothyronamine that mediate rapid physiologic effects of thyroid hormones suggested that such receptors may supplement the regulation of trh and trh-like peptides by nuclear t3 receptors. for this reason 200 g male sprague-dawley rats received daily i.p. injections of ptu or t4. levels of trh and trh-like peptides were measured 0, 2 h or 1, 2, 3, or 4 days later. rapid increases or decreases in trh and trh-like peptide levels ... | 2006 | 16310891 |
| comparison of dual-energy x-ray absorptiometry and conventional radiography for the detection of vertebral fractures. | prevalent vertebral fractures confer a high risk of subsequent fractures; therefore, fracture identification is a key issue in osteoporosis management. dual-energy x-ray absorptiometry has become the most widespread technique for the assessment of bone density, and can also provide images for assessment of vertebral morphometry (mxa) that are undistorted by parallax. the radiation dose is very low compared with conventional radiography. whether the resolution of mxa is adequate for vertebral mor ... | 2005 | 16311421 |
| [critical appraisal: combined t3 and t4 replacement therapy is not better than replacement with t4 alone]. | conflicting results have recently been published about the benefits of combined thyroxine (t4) and triiodothyronine (t3) in treating hypothyroid patients. however these studies may have been underpowered to detect differences in psychological well-being specifically related to thyroxine replacement. we conducted a large, double-blind, randomized controlled trial of partial substitution of 50 microg of t4 by 10 microg of t3 (t3) vs placebo (t4 alone - 50 microg of t4 replaced) in 697 hypothyroid ... | 2005 | 16311707 |
| pyridoxal phosphate inhibits the group i intron splicing. | the coenzyme pyridoxal phosphate and its analogs were tested for inhibition of the self-splicing of primary transcripts of the phage t4 thymidylate synthase gene (td). of all compounds examined, the pyridoxal phosphate was the most potent inhibitor and the order of inhibitory efficiency for compounds tested was as follows: pyridoxal phosphate > pyridoxal > pyridoxine > pyridoxamine > pyridoxic acid. increasing mg2+ concentration up to 14 mm overcame the suppression of self-splicing by pyridoxal ... | 2005 | 16311901 |
| extended resection of t4 lung cancer with invasion of the aorta: is it justified? | we report our 10-year experience of performing surgical resection of t4 lung cancer invading the thoracic aorta. | 2005 | 16311976 |
| using 2-aminopurine fluorescence to detect bacteriophage t4 dna polymerase-dna complexes that are important for primer extension and proofreading reactions. | the fluorescence of the base analogue 2-aminopurine (2ap) was used to probe bacteriophage t4 dna polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2ap fluorescence in dna is quenched by 2ap interactions with neighboring bases, but t4 dna polymerase binding to dna substrates labeled with 2ap in the templating position produces large increases in fluorescence intensity. fluorescence lifetime studies were perfor ... | 2005 | 16313170 |
| fertilization regulates apoptosis of ciona intestinalis extra-embryonic cells through thyroxine (t4)-dependent nf-kappab pathway activation during early embryonic development. | in ciona intestinalis, the elimination of extra-embryonic test cells during early stage of development is delayed by a fertilization signal. test cells undergo a caspase-dependent apoptosis event repressed by thyroxine (t4)-activated nf-kappab. when apoptosis was experimentally blocked, the hatching stage was delayed. the incubation of unfertilized eggs with a 1-h-fertilized egg extract or purified t4 restored apoptosis in test cells at a similar timing than found in fertilized eggs. ciona expre ... | 2006 | 16313896 |
| structure of a group a streptococcal phage-encoded virulence factor reveals a catalytically active triple-stranded beta-helix. | streptococcus pyogenes (group a streptococcus) causes severe invasive infections including scarlet fever, pharyngitis (streptococcal sore throat), skin infections, necrotizing fasciitis (flesh-eating disease), septicemia, erysipelas, cellulitis, acute rheumatic fever, and toxic shock. the conversion from nonpathogenic to toxigenic strains of s. pyogenes is frequently mediated by bacteriophage infection. one of the key bacteriophage-encoded virulence factors is a putative "hyaluronidase," hylp1, ... | 2005 | 16314578 |
| [a case report of complete obstruction of the left main bronchus caused by recurrent esophageal carcinoma with pulmonary embolism of right lung after surgery for esophageal cancer]. | a 54-year-old woman complained of dyspnea, due to complete obstruction of the left main bronchus caused by recurrent esophageal carcinoma, was transferred to our department about two months after curative resection (with preoperative chemotherapy for t4 cancer) in july 2004. she suddenly developed a severe shortness of breath with anxiety, and arterial blood gas analysis revealed a pao2 of 25 mmhg (fio2 1.0). the presence of pulmonary embolism was diagnosed by pulmonary perfusion scintigraphy. t ... | 2005 | 16315925 |
| fabricating rna microarrays with rna-dna surface ligation chemistry. | a novel surface attachment strategy that utilizes rna-dna surface ligation chemistry to create renewable rna microarrays from single-stranded dna (ssdna) microarrays on gold surfaces is demonstrated. the enzyme t4 dna ligase was used to catalyze the formation of a phosphodiester bond between 5'-phosphate-modified ssdna attached to the surface and the 3'-hydroxyl group of unlabeled rna molecules from solution in the presence of a complementary template dna strand. surface plasmon resonance imagin ... | 2005 | 16316195 |
| in situ preparation of protein-"smart" polymer conjugates with retention of bioactivity. | protein-polymer conjugates are widely used in biotechnology and medicine, and new methods to prepare the bioconjugates would be advantageous for these applications. in this report, we demonstrate that bioactive "smart" polymer conjugates can be synthesized by polymerizing from defined initiation sites on proteins, thus preparing the polymer conjugates in situ. in particular, free cysteines, cys-34 of bovine serum albumin (bsa) and cys-131 of t4 lysozyme v131c, were modified with initiators for a ... | 2005 | 16316241 |
| in vitro binding of anthrax protective antigen on bacteriophage t4 capsid surface through hoc-capsid interactions: a strategy for efficient display of large full-length proteins. | an in vitro binding system is described to display large full-length proteins on bacteriophage t4 capsid surface at high density. the phage t4 icosahedral capsid features 155 copies of a nonessential highly antigenic outer capsid protein, hoc, at the center of each major capsid protein hexon. gene fusions were engineered to express the 83-kda protective antigen (pa) from bacillus anthracis fused to the n-terminus of hoc and the 130-kda pa-hoc protein was expressed in escherichia coli and purifie ... | 2006 | 16316672 |
| [nasopharyngeal carcinoma in tunisian children: retrospective epidemiological, clinical and biological study about 48 cases]. | report epidemiological, clinical and biological aspects of nasopharyngeal carcinoma in tunisian children. | 2005 | 16316831 |
| prognostic value of dworak grade of regression (gr) in patients with rectal carcinoma treated with preoperative radiochemotherapy. | preoperative radiochemotherapy improves local control in locally advanced rectal cancer; however, its role in prolonging survival is still controversial. in order to better define the subset in patients who might benefit from this multimodal treatment, we have evaluated the correlation between grade of regression (gr) to preoperative treatment and disease-free survival (dfs). | 2006 | 16317549 |
| [auricular acupuncture combined with iodine salt for treatment of juvenile endemic goitre]. | to observe clinical therapeutic effect of auricular acupuncture combined with iodine salt on juvenile endemic goitre, and regulative action on thyroid hormones. | 2005 | 16318129 |
| transient hypothyroxinemia of prematurity and histological chorioamnionitis. | transient hypothyroxinaemia of prematurity (thop) is a common condition of preterm infants whose causes remain unclear. we tested the hypothesis that thop is associated with histological chorioamnionitis (hca). | 2005 | 16318614 |
| racial comparisons of thyroid function and autoimmunity during pregnancy and the postpartum period. | to evaluate thyroid function and the prevalence of thyroid peroxidase (tpo) antibody and autoimmunity in african-american and white women during pregnancy and the postpartum period. | 2005 | 16319264 |
| [features of osteopenic syndrome in diffuse toxic goiter]. | to characterize action of thyroid hormones overproduction on bone mineral density. | 2005 | 16320681 |
| [incidence and predictors of thyroid dysfunction caused by long-term intake of amiodaron]. | to analyze occurrence of thyroid dysfunction due to regular long-term intake of amiodaron (for one year), to search for predictors of amiodaron-induced hypothyroidism and thyrotoxicosis. | 2005 | 16320682 |
| synthesis and metabolism of thyroid hormones is preferentially maintained in selenium-deficient transgenic mice. | the thyroid gland is rich in selenium (se) and expresses a variety of selenoproteins that are involved in antioxidative defense and metabolism of thyroid hormones (th). se deficiency impairs regular synthesis of selenoproteins and adequate th metabolism. we recently generated mice that lack the plasma se carrier, selenoprotein p (sepp). sepp-knockout mice display decreased serum se levels and manifest growth defects and neurological abnormalities partly reminiscent of thyroid gland dysfunction o ... | 2006 | 16322066 |
| high antidiuretic hormone levels and hyponatremia in children with gastroenteritis. | nonosmotic antidiuretic hormone (adh) activity can cause severe hyponatremia during involuntary fluid administration. we looked for evidence of this before and during intravenous (iv) fluid administration in children treated for gastroenteritis. | 2005 | 16322164 |
| cognitive functioning and well-being in euthyroid patients on thyroxine replacement therapy for primary hypothyroidism. | hypothyroidism is associated with neurocognitive impairment. sparse data suggest that treatment of hypothyroidism, resulting in a return to euthyroidism, may be associated with only partial recovery of overall neurocognitive functioning. the aim of this study was to assess neurocognitive functioning and well-being in euthyroid patients with primary hypothyroidism on adequate thyroxine (t4) treatment. we also investigated whether serum tsh and thyroid antibodies are determinants of neurocognitive ... | 2005 | 16322379 |
| modification of erucic acid content in indian mustard (brassica juncea) by up-regulation and down-regulation of the brassica juncea fat ty acid elongation1 (bjfae1) gene. | in brassicas, the fatty acid elongation1 (fae1) gene product, a 3-ketoacyl-coa synthase, is the first in a 4-enzyme complex involved in the synthesis of erucic acid from oleic acid. the fae1 homologue from brassica juncea cv. pusa bold was cloned in a binary vector both in sense and antisense orientations under the control of the camv35s promoter. the recombinant binary vectors were used to transform b. juncea cv. rlm 198 via agrobacterium tumefaciens. the presence of the transgene was confirmed ... | 2006 | 16322995 |
| optimization of thyroxine replacement therapy after total or near-total thyroidectomy for benign thyroid disease. | total or near-total thyroidectomy is increasingly used to treat benign thyroid conditions. lifelong treatment with thyroxine (t4) is then required, but the optimal dose is difficult to predict. this study investigated factors that might predict the ideal t4 dose, with the aim of reducing delays in achieving normal thyroid function after surgery. | 2006 | 16323163 |
| dose-dependent transthyretin inhibition of t4 uptake from cerebrospinal fluid in sheep. | transthyretin (ttr), synthesized by the choroid plexuses (cp) has an important role in transporting thyroxine from blood to cerebrospinal fluid (csf). however, the role of ttr on thyroxine transport from csf to either blood or brain is not clear. by using the incubated isolated ovine brain tissues technique, we found the cp accumulated most 125i-t4 compared to ventricular ependymal, frontal cortex or cerebellum. the accumulation was higher in the young cp than the old. there was dose-dependent i ... | 2006 | 16325339 |
| ontogeny and effects of thyroid hormone on beta1-adrenergic receptor mrna expression in ovine fetal kidney cortex. | previous studies indicate that thyroidectomy (tx) decreases renin gene expression in ovine fetal renal cortex in late gestation. fetal ovine renin-containing renocortical cells become increasingly responsive to beta-adrenergic stimulation as gestation proceeds. increases in plasma thyroid hormone concentrations parallel this change, suggesting that there is a positive developmental relationship between the two. to examine this hypothesis, we determined the ontogeny of beta1-adrenergic receptor ( ... | 2005 | 16325745 |
| on association between cortical 5-ht2a receptors and behavior in rats with experimental thyroid disturbances. | thyroid hormones (th) were hypothesized to affect behavior via neurotransmission alterations. the present study was aimed to reveal effects of chronic th deficit and excess on some types of adaptive behavior (catalepsy, acoustic startle reflex, open-field performance), sexual arousal and cerebral 5-ht2a serotonin receptors of adult wistar rats. administration of thyroxine synthesis inhibitor, propylthiouracil (ptu, 50 mg/l, 28 days), in drinking water produced substantial decrease in plasma thyr ... | 2005 | 16325897 |
| fetal-to-maternal transfer of thyroid hormone metabolites in late gestation in sheep. | 3,3'-diiodothyronine sulfate (t2s) derived from t3 of fetal origin is transferred to the maternal circulation and contributes significantly to the maternal urinary pool. the present study quantitatively assesses the fetal to maternal transfer of t4 metabolites compared with those of t3. labeled t4 or t3 was infused intravenously to four singleton fetuses in utero in each group at gestational age 138 +/- 3 d. maternal and fetal serum and maternal urine samples were collected hourly for 4 h and at ... | 2006 | 16327008 |
| endocrine patterns in two strains of japanese black cattle with growth retardation. | endocrine patterns were compared in 2 strains of japanese black cattle with growth retardation; mho- and hsk-paternal strains (mho and hsk cattle, respectively). mho cattle (n=8) displayed lower serum concentrations of insulin-like growth factor-1 (igf-1), triiodothyronine (t3), thyroxine (t4), and cortisol (31.1+/-20.7 ng/ml, 73.9+/-51.9 ng/dl, and 2.9+/-2.9 microg/dl, 1.3+/-0.7 microg/dl, respectively) than those in both hsk cattle (n=5) (64.9+/-47.6 ng/ml, 97.8+/-40.7 ng/dl, 4.1+/-2.1 microg/ ... | 2005 | 16327227 |
| genomic instability analysis of urine sediment versus tumor tissue in transitional cell carcinoma of the urinary bladder. | microsatellite alterations are a common feature of neoplastic cells. our study aimed to compare the profile of microsatellite dna alterations in tumor tissue and urine sediment at 12 selected microsatellite loci in transitional cell carcinoma of the bladder, and to determine which of the 12 markers or combination of markers has potential for the non-invasive diagnosis of bladder cancer. dna alterations were examined using microsatellite markers on chromosomes 2p, 3p, 8p, 9p, 9q, 12q, 13q, 17p an ... | 2006 | 16328066 |
| agouti-related protein-deficient mice display an age-related lean phenotype. | endogenous modulators of the central melanocortin system, such as the agouti-related protein (agrp), should hold a pivotal position in the regulation of energy intake and expenditure. despite this, agrp-deficient mice were recently reported to exhibit normal food intake, body weight gain, and energy expenditure. here we demonstrate that 2- to 3-month-old agrp null mice do in fact exhibit subtle changes in response to feeding challenges (fasting and mcr agonists) but, of more significance and mag ... | 2005 | 16330327 |
| comparative analysis of thyroxine distribution in ascidian larvae. | the ascidian endostyle is a mucus-secreting pharyngeal organ, it has iodine-concentrating activity and the biosynthesis of thyroid hormones has been well documented. according to our recent findings, ascidians possess thyroid hormones, which are localized in mesenchymal cells. we have studied the presence and localization of l: -thyroxine (t(4)) in ascidia malaca (traustedt), ascidiella aspersa (müller), phallusia mamillata (cuvier) and ciona intestinalis (linnaeus) larvae and its involvement in ... | 2006 | 16331472 |
| chronic treatment with low doses of estradiol affects pituitary and thyroid function in young and middle-aged ovariectomized rats. | menopause marks the end of the reproductive life span of women and is characterized by a dramatic drop of circulating estrogen. hormone replacement therapy (hrt) has been successfully used to treat the symptoms of menopause because estrogen has strong suppressive effects on climacteric complaints. as the hypothalamo-pituitary-thyroid axis is a target for estrogen action we assessed the effect of ovariectomy (ovx) and long-term (3 months) estradiol (e2) treatment by means of subcutaneously (s.c.) ... | 2005 | 16333760 |
| [surgical treatment of hypopharyngeal cancer with cervical esophageal invasion]. | to review the experience of different surgical construction methods for hypopharyngeal cancer with cervical esophageal invasion. | 2005 | 16335405 |
| analysis of protein expression during oxidative stress in breast epithelial cells using a stable isotope labeled proteome internal standard. | normal cells undergo a variety of molecular and physiological changes upon malignant transformation, including their responses to environmental factors that induce oxidative stress. understanding the molecular pathways regulating these changes would facilitate the development of novel cancer treatments and chemoprevention strategies. differences in the oxidative stress response were investigated between nonmalignant (s-1) and malignant (t4-2) cell lines (both derived from the hmt-3522 breast epi ... | 2005 | 16335945 |
| testis structure and function in a nongenetic hyperadipose rat model at prepubertal and adult ages. | there are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium l-glutamate (msg). in our study, newborn male pups were ip injected with msg (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. plasma levels of leptin, lh, fsh, prolactin, testosterone (t), corticosterone, and free t4 (ft4) were measured. msg rats displayed elevated circulating levels of corticosterone and ... | 2006 | 16339210 |