| inhibitory effects of acyclic nucleoside phosphonate analogs, including (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, on epstein-barr virus replication. | (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(s)-hpmpa], (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)-2,6-diaminopurine [(s)-hpmpdap], cyclic (s)-hpmpa [(s)-chpmpa], 9-(2-phosphonylmethoxyethoxyethyl)-2,6-diaminopurine (pmedap), and (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine [(s)-hpmpc] were examined for their inhibitory effects on epstein-barr virus (ebv) replication. the 50% effective concentrations for inhibition of viral dna replication were 0.16, 0.03, 2.0, 1.5, and 0.08 micr ... | 1991 | 1666500 |
| hu-pbl-scid mice: a model for human immune function, aids, and lymphomagenesis. | | 1991 | 1666627 |
| phenotypic and functional studies of human peripheral blood lymphocytes engrafted in scid mice. | cb.17 mice homozygous for the scid defect have been used as recipients of peripheral blood lymphocytes (pbl) from normal humans and from patients suffering from common variable immunodeficiency (cvi) types a and b. following intra-peritoneal injection of pbl, such mice become chimeric with human cells, as evidenced by the presence in their serum of human immunoglobulins, which persist for a number of months. under these conditions, b cells from cvi patients are also triggered to produce immunogl ... | 1991 | 1666628 |
| sequence characteristics of a gene in equine herpesvirus 1 homologous to glycoprotein h of herpes simplex virus. | a gene in equine herpesvirus 1 (ehv-1, equine abortion virus) homologous to the glycoprotein h gene of herpes simplex virus (hsv) was identified and characterised by its nucleotide and derived amino acid sequence. the ehv-1 gh gene is located at 0.47-0.49 map units and contains an open reading frame capable of specifying a polypeptide of 848 amino acids, including n- and c-terminal hydrophobic domains consistent with signal and membrane anchor regions respectively, and 11 potential sites for n-g ... | 1991 | 1666854 |
| immunological properties and differentiation potential of human colostral lymphocytes of b cell lineage. | | 1991 | 1667060 |
| the latent membrane protein oncoprotein resembles growth factor receptors in the properties of its turnover. | the latent membrane protein (lmp) of epstein-barr virus functions as an oncogene in rodent cell lines (d. wang, d. liebowitz, and e. kieff, cell, 43: 831-840, 1985; v. r. baichwal and b. sugden, oncogene, 2: 461-467, 1988) and, therefore, is likely to be essential for immortalization of human b-lymphocytes by epstein-barr virus. lmp has a short half-life in epstein-barr virus-infected b-lymphoblastoid cells (v. r. baichwal and b. sugden, j. virol., 61: 866-875, 1987; k. p. mann and d. thorley-la ... | 1991 | 1667088 |
| recognition of transformed cells by autologous lymphocytes: a review. | the studies on the t cell response against ebv carrying b cells demonstrated its high power in vivo. malignancies with phenotypes similar to the in vitro transformed b cells occur only in severely immunosuppressed patients. presently the goal of the studies is the identification of the antigens recognised by t lymphocytes and their association with the various hla alleles. the studies on human sarcomas and carcinomas still struggle with the demonstration of specific t cell responses and the char ... | 1991 | 1667269 |
| production of anti-tumor-promoting iridoid glucosides in genipa americana and its cell cultures. | the genipa americana plant contains geniposide [3] and geniposidic acid [2] in the fruits and geniposidic acid [2] in the leaves. on callus induction, the plant produces tarennoside [1], geniposidic acid [2], and gardenoside [4] in high levels. the leaves of ge. americana plants redifferentiated from the callus tissues produce 2. | 1991 | 1667413 |
| malignant lymphomas in japanese aids patients. | seven non-hodgkin's malignant lymphomas (mls) were found in 25 japanese aids (acquired immune deficiency syndrome) patients who died at two hospitals in tokyo. all of these mls originated from extranodal organs including the brain (three cases), skin (two cases) liver and adrenal gland. b cell markers were demonstrated in all of them. epstein-barr virus (ebv) capsid antigen (ebvca) was demonstrated in 5/7, nuclear antigen (ebna) in 2/2, the w fragment of ebv dna by in situ hybridization in 5/7 a ... | 1991 | 1667449 |
| a preliminary study on the immunogenicity in rabbits and in human volunteers of a recombinant vaccinia virus expressing epstein-barr virus membrane antigen. | | 1991 | 1667485 |
| [renal tubular acidosis in primary sjogren's syndrome: study on the immunology and ultrastructure of ebv]. | the related antigens of ebv were examined by mcab against epstein-barr virus eap 138. the related antibody of serous ebv vca and ebna of sjogren's syndrome (ss) patients was examined by indirect immunofluorescence and immunoenzyme methods. ebv was found in the tubular epithelial cells by em and the relationship between ebv and ss was studied. the results showed that (1) around the nuclei and basement membrane of the proximal tubular epithelial cells there were positively reacting granules but no ... | 1991 | 1667508 |
| pulmonary infections in the immunocompromised host. | the expression "immunocompromised host" refers to an individual who has one or more defects in the body's natural defense, which leads to severe, often life-threatening, infections. alcoholism, diabetes mellitus, advanced age, the use of antacids, and viral infections have immune-modulating effects. the human immunodeficiency virus, cytomegalovirus, epstein-barr virus, and non a, non b hepatitis virus also contribute to immunosuppression. the lung has a special vulnerability to infection, and ... | 1991 | 1667654 |
| development and evaluation of a capture elisa for igm antibody to the human cytomegalovirus major dna binding protein. | a new capture elisa (elab) for determination of the igm antibody response to the human cytomegalovirus major dna binding protein (p52) was developed by using a p52-specific monoclonal antibody. as a reference test, a capture elisa using in parallel viral- and cell-control labeled antigens (ela) was employed. general specificity, which was determined on 180 unselected igm-negative sera from an adult population was 100%; stringent specificity, which was evaluated on 108 potentially interfering ser ... | 1991 | 1667791 |
| two t(9;22) leukemia cell lines (nalm-24 and nalm-25) with bi-phenotypic characteristics and an ebv-positive b-cell non-leukemia cell line (b262) established from a patient with acute lymphoblastic leukemia. | based on the immunophenotypic, cytogenetic and genotypic findings, two unique leukemia cell lines, nalm-24 and nalm-25, and an ebv-transformed "normal" b-lymphoblastoid cell line (b262) from a patient with all were established and characterized. nalm-24 and nalm-25 are unique in that expression of both show b cell and myeloid cell features with the t(9;22) chromosome in single clonal leukemic cell populations. | 1991 | 1667895 |
| aetiology and pathogenesis of non-hodgkin lymphoma in aids. | the aids-nhls constitute a histologically diverse group of tumours. comparisons with some groups of non-aid-nhls, including ebl, sbl and large cell lymphoma, have been made and similar pathogenetic mechanisms postulated for such groups. however, the incidence of the different pathological types of nhl in aids is not yet clear, and further work is necessary to determine the contribution of factors such as c-myc translocations and the presence of ebv. aids-nhls are clearly a more diverse group of ... | 1991 | 1668231 |
| epstein-barr virus dna and latent gene products in ki-1 (cd30)-positive anaplastic large cell lymphomas. | epstein-barr virus (ebv)-specific dna sequences were detected by polymerase chain reaction analysis in 15 of 47 (32%) dna extracts prepared from cd30-positive (ki-1 antigen-positive) anaplastic large cell (alc) lymphomas. ebv-encoded rna (eber) transcripts could be detected by in situ hybridization in the tumor cells of 9 of 11 ebv dna-positive cases. twenty-eight cases were examined by immunohistology on cryostat sections for the presence of the ebv-encoded latent membrane protein (lmp), the nu ... | 1991 | 1668608 |
| human gamma delta t cells amplify ige production by epstein-barr virus-activated b cells. | in summary, these data document that gamma delta t cells regulate b cell differentiation, antibody synthesis, and ige secretion in ebv-activated b cells. the classical "helper" subset of cd4+ alpha beta t cells, although potent inducers of other ig isotypes, are not as efficient in augmenting ige synthesis. however, interactions between alpha beta and gamma delta cells enhance the ige response further. the precise mechanism by which gamma delta t cells function to augment ige secretion is under ... | 1991 | 1668986 |
| [hepatitis by infectious mononucleosis]. | five adult patients presenting with clinical and laboratory manifestations of an acute hepatitis in the course of a hitherto undiagnosed infectious mononucleosis (im) are reviewed. chief complaints were intense malaise and prolonged fever (7 to 15 days prior to diagnosis). serum aminotransferases were moderately raised in all patients; three patients had mild jaundice with a direct-reacting hyperbilirubinemia; 4 patients had an enlarged and tender liver. when making the differential diagnosis of ... | 1991 | 1668992 |
| dual infections of the immune system in patients with chronic active epstein-barr virus infection mimicking chronic fatigue syndrome. | the etiologic bases of cfs are undetermined at the present time. it is very important to distinguish the patients with cfs as defined by the centers for disease control (cdc) case definition of holmes et al. from patients with physical and laboratory findings suggesting dual infections and/or underlying immunodeficiency. particularly fruitful might be a longitudinal immunovirologic study of patients who exhibit cfs following a well-documented viral infection. | 1991 | 1669350 |
| search for an association between epstein-barr virus infection and the chronic fatigue syndrome. | | 1991 | 1669352 |
| virology laboratory diagnosis of chronic fatigue syndrome. | | 1991 | 1669355 |
| viruses and cancer. causal associations. | this review first considered some general problems in establishing causal links between a virus and a human cancer and offered some guidelines in the pursuit of this objective. second, it reviewed the current causal associations for several candidate oncogenic viruses in relation to the tumors with which they are associated. these include epstein-barr virus in relation to burkitt's lymphoma, nasopharyngeal carcinoma, hodgkin's disease, and non-hodgkin's lymphoma; hepatitis b and c viruses in rel ... | 1990 | 1669491 |
| epstein-barr virus transmission via the donor organs in solid organ transplantation: polymerase chain reaction and restriction fragment length polymorphism analysis of ir2, ir3, and ir4. | two organ transplant recipients who received organs from a common donor and were diagnosed as having an epstein-barr virus (ebv)-associated posttransplant lymphoproliferative disorder were studied to determine the mode of ebv transmission. the results of restriction fragment length polymorphism, polymerase chain reaction, and minisatellite dna analyses demonstrate that both patients had a common strain of ebv and that this strain was transmitted from the donor's organs to both recipients. posttr ... | 1991 | 1670959 |
| expression of epstein-barr virus latent gene products in tumour cells of hodgkin's disease. | the epstein-barr virus (ebv)-encoded latent gene products, latent membrane protein (lmp) and ebv nuclear antigen 2 (ebna 2), seem to have important roles in ebv-induced cell transformation in vitro, and have been implicated as important effector molecules in ebv-associated lymphomagenesis. because up to 35% of hodgkin's disease (hd) samples have been reported to contain ebv genomes, the expression of lmp and ebna 2 in these tumours was investigated. 84 cases of hd were studied with monoclonal an ... | 1991 | 1671232 |
| diagnosis of nasopharyngeal carcinoma by means of recombinant epstein-barr virus proteins. | the immune response of patients with nasopharyngeal carcinoma to epstein-barr virus (ebv) antigens is diagnostic of the tumour. existing tests use ebv antigens produced in ebv-infected lymphoblastoid cells, but the virus replicates poorly in these cells. serum samples from 18 patients diagnosed as having nasopharyngeal carcinoma were screened by western blot analysis, enzyme-linked immunosorbent assay (elisa), and immunofluorescence tests for antibodies to the ebv-coded alkaline deoxyribonucleas ... | 1991 | 1672175 |
| expression of cd45r0 (uchl1) by cd4+ and cd8+ t cells as a sign of in vivo activation in infectious mononucleosis. | cd45r0 (uchl1), a member of leucocyte common antigen family, is expressed largely on previously activated or memory t cells. we examined cd45r0 expression of t cell subpopulations in patients with epstein-barr virus (ebv) induced infectious mononucleosis (imn) as a sign of in vivo activation. consistent with the notion that activated cd8+ t cells expand in acute imn; the majority of cd8+ t cells in patients with acute imn expressed cd45r0 to the similar extent to hla-dr expression. most cd4+ t c ... | 1991 | 1672270 |
| retroviral sequences related to human t-lymphotropic virus type ii in patients with chronic fatigue immune dysfunction syndrome. | chronic fatigue immune dysfunction syndrome (cfids) is a recently recognized illness characterized by debilitating fatigue as well as immunological and neurological abnormalities [straus, s.e. (1988) j. inf. dis. 157, 405-412]. once thought to be caused by epstein-barr virus, it is now thought to have a different but unknown etiology. we evaluated 30 adult and pediatric cfids patients from six eastern states for the presence of human t-lymphotropic virus (htlv) types i and ii by western immunobl ... | 1991 | 1672770 |
| aids-associated non-hodgkin lymphoma. | non-hodgkin lymphoma is associated with hiv infection. we investigated the epidemiology and aetiology of aids-related non-hodgkin lymphoma by analysing data from cases reported to the centers for disease control, atlanta, usa, up to june 30, 1989. during this period 97,258 aids cases were reported, of whom 2824 (2.9%) had non-hodgkin lymphoma. the condition was about 60 times more common in aids patients than in the general us population. 1686 cases were immunoblastic lymphoma, 548 primary lymph ... | 1991 | 1672911 |
| circulating epstein-barr virus-carrying b cells in acute malaria. | epstein-barr virus (ebv) infection and plasmodium falciparum malaria are two known cofactors in the aetiology of endemic burkitt's lymphoma. to assess the relation between these factors, limiting dilution analysis was used to assess the number of ebv-carrying b cells in the circulation of gambian children during and after acute malaria. numbers of virus-carrying cells were five times higher in acute malaria patients and in uk patients with infectious mononucleosis than in convalescent malaria pa ... | 1991 | 1672968 |
| binding of epstein-barr virus small rna eber-1 to the double-stranded rna-activated protein kinase dai. | epstein-barr virus encodes two small rnas, eber-1 and -2, that are abundantly expressed in latently infected cells. recent evidence suggests a role for eber-1 in regulation of translation since this rna is able to prevent the inhibition of protein synthesis by double-stranded rna in rabbit reticulocyte lysates. we show here that eber-1 that has been synthesized in vitro forms a complex with the dsrna-activated inhibitor of protein synthesis dai, a protein kinase that specifically phosphorylates ... | 1991 | 1673026 |
| epstein-barr virus type b in patient with t-cell lymphoma. | | 1991 | 1673201 |
| absence of epstein-barr virus nuclear antigen 2 in tumour cells of hodgkin's disease. | | 1991 | 1673537 |
| two patients with insulin resistance due to decreased levels of insulin-receptor mrna. | mutations have been identified in the insulin-receptor gene in insulin-resistant patients. we studied two patients with acanthosis nigricans and insulin resistance caused by a decrease in the number of cell surface insulin receptors. patient 1 was an 11-yr-old boy with a fasting insulin level of 2130 pm; patient 2 was a 14-yr-old girl with hyperandrogenism and a fasting insulin level of 580-740 pm. based on southern-blotting studies, the structure of both alleles of the insulin-receptor gene in ... | 1991 | 1673662 |
| chromosome translocation and c-myc activation by epstein-barr virus and euphorbia tirucalli in b lymphocytes. | dual exposure to epstein-barr virus and purified 4-deoxyphorbol ester derived from the plant euphorbia tirucalli induced a high frequency of chromosomal rearrangements in human b lymphocytes in vitro. rearrangements most commonly affected chromosome 8, the chromosome most often showing structural changes in burkitt's lymphoma (bl) cells. e tirucalli is indigenous in parts of africa where bl is endemic and may be an important risk factor for the disease. | 1991 | 1673740 |
| epstein-barr virus latent genes in tumour cells of hodgkin's disease. | | 1991 | 1675752 |
| viral and cellular gene expression in cd4+ human lymphoid cell lines infected by the simian immunodeficiency virus, siv/mne. | our laboratory has undertaken an analysis of cellular and viral gene expression in cd4+ human lymphoid cell lines infected by the human and simian immunodeficiency viruses, hiv-1 and siv/mne, respectively. the purpose of the current study was to: (i) examine the effects of siv/mne infection on host macromolecular synthesis and compare the results to those in the hiv-1 system; and (ii) investigate the mechanisms responsible for the restriction of siv/mne infection in cd4 positive lymphoid cells w ... | 1991 | 1675822 |
| characterization of icam-2 and evidence for a third counter-receptor for lfa-1. | in an endeavor to further characterize human intercellular adhesion molecule-2 (icam-2), two murine monoclonal antibodies (mab) were generated to icam-2 transfected cos cells, and designated cbr-ic2/1 and cbr-ic2/2. immunoprecipitated, reduced icam-2 migrated as a broad band of mr 60,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. treatment with n-glycanase revealed a peptide backbone of mr 31,000, consistent with the size predicted from the cdna. icam-2 had a broad distributio ... | 1991 | 1676048 |
| loss of heterozygosity on the short arm of chromosome 3 in nasopharyngeal carcinoma. | a consistent loss of constitutional heterozygosity within a specific chromosome locus in a tumor type is suggestive of a tumor suppressor gene important in the genesis of that tumor. we studied whether such genetic alterations are involved, in the development of nasopharyngeal carcinoma (npc). tumor and matched blood leukocytes dna from eleven hong kong chinese patients with primary npc stages i to iv were subjected to restriction fragment length polymorphism (rflp) analysis using chromosome 3-s ... | 1991 | 1676610 |
| histiocytic medullary reticulosis, a lethal form of epstein-barr-virus-related disorder. | | 1991 | 1678075 |
| mononucleosis and hepatic failure associated with diphenylhydantoin treatment in an infant. | diphenylhydantoin-induced hepatitis and mononucleosis are uncommon in children. the occurrence of these two diseases in the same individual, with progression to hepatic failure is rare and has not been reported in infants. this report represents a 6-month-old male infant who developed an infectious mononucleosis-like syndrome and hepatic failure 16 days after diphenylhydantoin administration. he took this anticonvulsant for controlling seizures after a head injury. fever, skin rash, hepatospleno ... | 1991 | 1678417 |
| epstein-barr virus transactivator antibodies in hiv infection. | | 1991 | 1678828 |
| replication timing control can be maintained in extrachromosomally amplified genes. | extrachromosomal elements are common early intermediates of gene amplification in vivo and in cell culture. the time at which several extrachromosomal elements replicate was compared with that of the corresponding amplified or unamplified chromosomal sequences. the replication timing analysis employed a retroactive synchrony method in which fluorescence-activated cell sorting was used to obtain cells at different stages of the cell cycle. extrachromosomally amplified syrian hamster cad genes (ca ... | 1991 | 1678857 |
| chronic fatigue syndrome: i. epstein-barr virus immune response and molecular epidemiology. | patients with chronic fatigue syndrome were compared to healthy seropositive control subjects in an open study and a case-control study analyzing spontaneous transformation rates of peripheral blood lymphocytes, ebv viral genome characteristics as determined by dna restriction fragment polymorphisms, and antibody production by western blot analysis. thirty percent of patients versus 8% of control subjects underwent spontaneous transformation in the two studies. viral genome patterns were overall ... | 1991 | 1679118 |
| the epstein-barr virus bnlf-1 membrane protein lmp1 induces homotypic adhesion mediated by cd11a/cd18 in a murine b-cell line, mimicking the action of phorbol ester. | expression of the epstein-barr virus bnlf1 gene (lmp1) or treatment with 4b-phorbol-12,13-dibutyrate in the murine pro-b-cell line a/j-95 leads to a five- to sevenfold enhancement of homotypic adhesion without significant changes in adhesion molecule expression. antibody to cd11a inhibits aggregation, indicating that cd11a/cd18-mediated adhesion is a common target for both agents. | 1991 | 1680199 |
| enhanced expression of hla-class ii molecules on activated human t lymphocytes following treatment with tumor necrosis factor alpha. | many factors induce or enhance expression of major histocompatibility complex class i and class ii molecules on various cell types. human t lymphocytes are class ii negative in the resting state but show expression of class ii molecules following activation. we analyzed the modulating capacity of the lymphokines recombinant interferon gamma (rifn-gamma), interleukin-4 (il-4), and recombinant tumor necrosis factor alpha (rtnf-alpha) on class ii expression in subsets of alloactivated human t lymph ... | 1991 | 1680840 |
| isolation and sequencing of the epstein-barr virus bnlf-1 gene (lmp1) from a chinese nasopharyngeal carcinoma. | the bamhi fragment containing the epstein-barr virus (ebv) lmp1 gene was cloned from a genomic library of the nude mouse-propagated chinese nasopharyngeal carcinoma cao. the sequence of the lmp1 gene and its promoter and enhancer was determined. the nucleotide sequence of the cao isolate differed from those of the b95-8 and raji isolates in the promoter/enhancer region; the amino acid sequence of the protein also differed. structural differences in the protein were located mainly in the 20 n-ter ... | 1991 | 1681026 |
| ["vaccination encephalopathy after measles vaccination simulating recurrent cardiovascular dysregulation?" the pediatric vaccination counseling unit]. | | 1991 | 1681130 |
| epstein-barr virus in aids-related primary central nervous system lymphoma. | primary central nervous system lymphoma occurs more often in patients with aids. epstein-barr virus (ebv) has been detected in these tumours, but the degree of association has not been defined because of both the highly restricted expression of ebv in malignant tissue and the lack of a technique that is reliable in formalin-fixed paraffin-embedded specimens. ebv-transformed lymphocytes contain short non-protein coding ebv transcripts (ebers), which are expressed in much higher quantity than othe ... | 1991 | 1681341 |
| epstein-barr virus and aids-associated lymphomas. | | 1991 | 1681345 |
| immunoglobulin class and subclass deficiencies prior to epstein-barr virus infection in males with x-linked lymphoproliferative disease. | patients with x-linked lymphoproliferative (xlp) disease are characterized by extreme vulnerability to epstein-barr virus (ebv). following infection with ebv, affected males develop fatal infectious mononucleosis (im), hypogammaglobulinemia (h), or non-hodgkin's lymphoma (nhl). in addition, hyper igm, red cell aplasia, necrotizing lymphoid vasculitis (nlv), and aplastic anemia occur rarely. the recent use of dna restriction fragment length polymorphism (rflp) probes in linkage with the xlp gene ... | 1991 | 1683154 |
| lack of bcr rearrangement in juvenile chronic myeloid leukemia. | juvenile chronic myeloid leukemia (jcml) is an unusual subtype of children's leukemia, characterized by unique clinical presentation. recent studies revealed several biological features, distinguishing from those observed in adult type chronic myeloid leukemia (acml). the majority of acml cases are characterized by the presence of an hybrid bcr-abl rearranged gene. in an effort to elucidate the molecular basis of this unusual leukemia we looked for bcr rearrangement in six jcml cases. no bcr rea ... | 1991 | 1683680 |
| scid/human mouse model of central nervous system lymphoproliferative disease. | epstein-barr virus (ebv) carrying malignant lymphomas of the central nervous system are increasing owing to the increasing numbers of immunodeficient patients. to further understand the pathogenesis of these tumors, we studied the invasiveness of four ebv-immortalized lymphoblastoid cell lines in the brains of immunodeficient mice and the expression of adhesion molecules during this process. we injected four ebv-infected human lymphoblastoid cell lines intracerebrally into nude as well as scid/s ... | 1991 | 1684402 |
| fatal b-cell lymphoproliferative syndrome in allogeneic marrow graft recipients. a clinical, immunobiological and pathological study. | we have studied four cases of fatal b-cell lymphoproliferative syndrome (lps) developing among 333 patients (incidence 1.2%) treated with allogeneic bone marrow transplantation (bmt). all four patients had received a t-cell depleted graft. onset of the first clinical symptoms (palpable lymph node enlargement in three and iga-lambda paraproteinemia in two patients) occurred between 41 and 188 days post-bmt (median 76 days). the course of the lps was rapidly progressive in all cases, leading to de ... | 1991 | 1685038 |
| epstein-barr virus infection and neurologic disease. | | 1991 | 1685230 |
| lymphadenitis and lymphoproliferative lesions associated with the human herpes virus-6 (hhv-6). | a newly described herpes virus, human herpes virus 6, (hhv-6), has been linked to exanthema subitum but beyond this its pathogenetic impact remains to be determined. a large body of evidence links it to various lymphoproliferative disorders and this study was conducted to identify forms of lymphoproliferation linked to hhv-6. we studied biopsy samples from 32 patients with disorders of the lymphatic system for the presence of hhv-6, both by polymerase chain reaction (pcr) and in-situ hybridizati ... | 1991 | 1685279 |
| arylsulfatases a and b in ebv-transformed lymphoid cell lines: studies on their molecular forms in cells from patients with inborn sulfatase deficiencies. comparative diagnostic value of enzymatic assays. | the enzyme activity of arylsulfatase a and arylsulfatase b was studied in epstein-barr virus-transformed lymphoid cell lines established from control individuals and patients affected with metachromatic leukodystrophy, mucopolysaccharidosis type vi (or maroteaux-lamy syndrome) and multiple sulfatase deficiency. lymphoid cells derived from patients with metachromatic leukodystrophy showed a severe deficiency in cerebroside sulfatase activity, as measured using radiolabelled sulfatide, but some re ... | 1991 | 1687673 |
| cross-reactivity between the ebna-1 p107 peptide, collagen, and keratin: implications for the pathogenesis of rheumatoid arthritis. | an unusually heavy load of epstein-barr virus (ebv) infection and autoimmunity to collagen are believed to be contributing factors to the pathogenesis of rheumatoid arthritis (ra). the present report presents data showing that p107, the major epitope of the ebv-encoded ebna-1 antigen, cross-reacts with denatured collagen (dc) and keratin (k), suggesting a new likely link among ra, ebv-1, and these autoantigens. a radioimmunoassay using antigen-coated microtiter plates was used to demonstrate ant ... | 1990 | 1688406 |
| cold agglutinins: specificity, idiotypy and structural analysis. | cold agglutinins likely represent natural autoantibodies with precursors that arise early during ontogeny. many use l chains derived from the germline kv325 gene, in association with h chains derived from the vhiv gene family. cas probably represent an expression of the preimmune repertoire. the appearance of cas in a variety of disorders may be due to the prevalence of ca b cell precursors, and the ubiquity of their target epitopes. cas may arise independent of antigenic selection during epstei ... | 1990 | 1688493 |
| an epstein-barr virus-specific cytotoxic t cell epitope in ebv nuclear antigen 3 (ebna 3). | epstein-barr virus (ebv)-specific ctl clones were isolated that recognized a-type ebv transformants but not b-type transformants. these a-type-specific ctl clones (hla b8 restricted) were used to screen peptides derived from the ebv nuclear antigens (ebnas) 2, 3, 4, and 6 as potential ctl epitopes. of the 76 peptides screened, one sequence from ebna 3 (residues 329-353) was recognized by a-type-specific ctl clones after absorption onto target cells (either autologous b-type transformants or pha ... | 1990 | 1688611 |
| circulating immune complexes in patients with nasopharyngeal carcinoma. | studies of the serum of patients with nasopharyngeal carcinoma (npc) demonstrate increased levels of immunoglobulins (igs), epstein-barr virus (ebv) antibodies, and specific immunosuppressive acute-phase proteins (haptoglobin, alpha 1-acid glycoprotein). recent correlations of the levels of acute-phase and immune-reactive proteins with tumor extent, immune reactivity, and circulating immune complex (cic) levels in patients with head and neck squamous carcinoma prompted an investigation of the re ... | 1990 | 1689798 |
| stable expression and function of ebv/c3d receptor following genomic transfection into murine fibroblast l cells. | the epstein-barr virus (ebv) and the c3d component of complement bind to the same cell surface receptor (ebvr/cr2) which is part of the b lymphocyte differentiation antigen recognized by the monoclonal antibodies (mab) of the cluster of differentiation 21 (cd21). to analyze ebv and c3d binding to this receptor, mouse fibroblasts were transfected with human genomic dna and rare cd21-positive cells were selected and cloned by cell sorting. the presence of the human gene in host cell dna as well as ... | 1990 | 1690137 |
| high complete response in advanced nasopharyngeal carcinoma with bleomycin, epirubicin, and cisplatin before radiotherapy. | undifferentiated carcinoma of nasopharyngeal type (ucnt) is a geographically endemic, epstein-barr virus-related carcinoma of epidermoid origin with reported 5-year survival rates of 15%-40% when treated with radiotherapy alone. although ucnt can be well controlled locally by radiation therapy, in advanced nodal stage n3 [international union against cancer-american joint committee on cancer (uicc-ajcc, 1987)] the survival rate is below 20%, primarily because of metastatic spread in 80% of the fa ... | 1990 | 1690306 |
| rna sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection. | although dideoxy terminated sequencing of rna, using reverse transcriptase and oligodeoxynucleotide primers, is now a well established method, the accuracy is limited by sequence ambiguities due to unspecific chain termination events. a protocol is described which circumvents these ambiguities by using fluorescence labels tagged to dideoxynucleotides. only chain terminations caused by dideoxynucleotides were detected while premature terminated cdna's remain undetectable. in addition, the remaini ... | 1990 | 1690393 |
| an epitope in human immunodeficiency virus 1 reverse transcriptase recognized by both mouse and human cytotoxic t lymphocytes. | t-cell-mediated cytotoxicity may play an important role in control of infection by the human immunodeficiency virus (hiv). in this study, we have identified and characterized a relatively conserved epitope in the hiv-1 reverse transcriptase recognized by murine and human cytotoxic t cells. this epitope was identified using a murine antigen-specific cd8+ class i major histocompatibility complex-restricted cytotoxic t-cell (ctl) line, a transfected fibroblast cell line expressing the hiv-1 pol gen ... | 1990 | 1690429 |
| in situ hybridization in hematopathology. | in situ hybridization (ish) is one of the molecular techniques that has applications in diagnostic hematopathology. this procedure allows the detection of dna or rna in intact cells from various preparations, including cytology specimens and routinely fixed paraffin-embedded tissues. ish is therefore analogous to detecting proteins (antigens) in intact cells with immunohistochemistry. the purpose of this article is to review the basic concepts and principles of ish and to briefly discuss the imp ... | 1990 | 1690505 |
| cd5 and immunoglobulin v gene expression in b-cell lymphomas and chronic lymphocytic leukemia. | we studied the expression of cd5 and immunoglobulin variable gene families in a panel of monoclonal epstein-barr virus (ebv) transformed lines, chronic lymphocytic leukemias (clls) and cd5+ and cd5- b-cell lymphomas. the cd5 gene expression was in all cases identical to that of t-cell malignancies. the utilization of the various vh and vk gene families was roughly proportional to the estimated gene family size in ebv lines obtained from adult healthy subjects. in contrast we found a statisticall ... | 1990 | 1690580 |
| patterns of reactivity of epstein-barr virus-specific t cells in a-type donor cultures after reactivation with autologous a- or b-type transformants. | regression endpoints were assessed in cultures from 11 epstein-barr (eb) virus a-type seropositive donors and 2 seronegative donors using a- and b-type eb virus preparations. in 9/11 of the seropositive donors, the resulting endpoints using a-type or b-type virus were similar and demonstrated a significant t cell response to both virus types. however, the regression endpoints for 2/11 seropositive donors were reproducibly higher with b-type virus compared with a-type virus, indicating a weak t c ... | 1990 | 1690609 |
| absence of epstein-barr virus-specific, hla class ii-restricted cd4+ cytotoxic t lymphocytes in infectious mononucleosis. | cytotoxic t lymphocytes (ctl) with the cd4+ phenotype that recognize major histocompatibility complex (mhc) class ii antigens are detectable very frequently in cultures of human alloreactive or virus-specific t cells. the significance of these cd4+ ctl for an immune reaction in vivo is not clear. since epstein-barr virus (ebv) transformed b cells express hla-class i and class ii antigens equally well both cd8+ and cd4+ ctl should be stimulated during an acute ebv infection. we analysed the mhc s ... | 1990 | 1690617 |
| cross-recognition of a mouse h-2-peptide complex by human hla-restricted cytotoxic t cells. | epstein-barr virus (ebv)-specific human cytotoxic t lymphocyte (ctl) lines from a virus-immune hla-a11+ donor cmc produced significant lysis of transfected mouse p815 target cells (h-2d background) expressing an introduced hla-a11 heavy chain gene and the ebv gene encoding latent membrane protein (lmp). to identify the target epitope being recognized, hla-a11+ transfectants of p815/a11) were pre-exposed to synthetic peptides corresponding to fragments of the lmp sequence and the cells then teste ... | 1990 | 1690661 |
| evaluation of individual specificities of class i hla on platelets by a newly developed monoclonal antibody. | in order to quantify each specific hla-a or -b antigen on platelets, a monoclonal antibody against hla heavy chains was developed and designated as 2f2 monoclonal antibody. this monoclonal antibody reacted on western blot with platelet hla from each of 10 individuals with different hla phenotypes and precipitated all 35s-methionine-labeled hla-a and -b antigens from three different epstein-barr virus--transformed lymphoblastoid cell lines. the results indicate that the 2f2 monoclonal antibody re ... | 1990 | 1690692 |
| disease-associated human histocompatibility leukocyte antigen determinants in patients with seropositive rheumatoid arthritis. functional role in antigen-specific and allogeneic t cell recognition. | the susceptibility to develop seropositive rheumatoid arthritis (ra) has been linked to specific genomic polymorphisms within the hla complex. two different haplotypes have been associated with the disease, hla-dr1 and hla-dr4. to investigate the link between such phenotypic disease associations and potential immune mechanisms we used alloreactive and antigen-specific human t cell clones. here we describe a panel of alloreactive t cell clones directed to polymorphic determinants encoded by the t ... | 1990 | 1690755 |
| cd5 mrna expression and auto-antibody production in early human b cells immortalized by ebv. | a number of studies have suggested that lymphocytes producing polyreactive antibodies belong to the cd5+ b-cell subset. in this study we have examined cd5 at the cell surface and mrna levels in ebv-driven cord blood and fetal liver clones previously characterized in terms of their antibody specificities. we show that ebv-immortalized cells can express surface cd5, and that some of the clones not expressing surface cd5 express it at the mrna level. the complete absence of cd5 mrna in some polyrea ... | 1990 | 1690914 |
| bidirectional transcription of the epstein-barr virus major internal repeat. | in latently infected cell lines, the epstein-barr virus bamhi w fragment (major internal repeat) is transcribed in a rightward direction to yield exons common to several alternatively spliced messages which encode the six known viral nuclear antigens. a substantial steady-state population of very large (up to 20-kilobase) rightward transcripts is nuclear, much of it being polyadenylated. we report a rise in the levels of rightward transcripts hybridizing to bamhi-w sequences upon phorbol ester t ... | 1990 | 1691315 |
| [polyclonal activated circulating b-lymphocytes in patients with rheumatoid arthritis]. | peripheral b lymphocytes were studied to investigate the role of polyclonal b-cell activation in the pathogenesis of rheumatoid arthritis (ra). proliferative responses to anti-mu antibodies coupled to sepharose used as a b-cell-specific mitogen were reduced as compared to healthy controls. when cell surface antigens were studied by flow cytometry employing monoclonal antibodies, b cells from patients with ra showed enhanced expression of interleukin-2 (il-2) receptor. in-vivo-activated, in-vitro ... | 1990 | 1691568 |
| different epstein-barr virus expression in lymphomas from immunocompromised and immunocompetent patients. | eighteen tissue samples from lymphoproliferative lesions and lymphomas in immunodeficiency states were investigated for their content of epstein-barr virus (ebv) genome by dot blotting and for the distribution of ebv in tissue sections by in situ hybridization. fourteen lymphomas from aids patients and four children with disorders of the immune system were available. for control reasons, six cases of infectious mononucleosis (im) and eight burkitt's lymphomas (bl) from malaria-free regions of af ... | 1990 | 1691592 |
| low expression of lymphocyte function-associated antigen (lfa)-1 and lfa-3 adhesion molecules is a common trait in burkitt's lymphoma associated with and not associated with epstein-barr virus. | lymphocyte function-associated antigens 1 and 3 (lfa-1, lfa-3) and intercellular adhesion molecule 1 (icam-1) are cell surface adhesion molecules necessary for immune processes requiring intercellular contact. it was recently proposed that malignant burkitt's lymphoma cells (bl) may escape from immunosurveillance through the downregulation of lfa-1 (cd11a/cd18) or lfa-3 (cd58) and icam-1 (cd54) molecules. expression of these three adhesion antigens was investigated in 19 bl lines. lfa-1 or lfa-3 ... | 1990 | 1691936 |
| characterization of the gene and an antigenic determinant of equine herpesvirus type-1 glycoprotein 14 with homology to gb-equivalent glycoproteins of other herpesviruses. | the gene encoding glycoprotein 14 (gp14) of equine herpesvirus type 1 was sequenced. nucleotide sequence analysis revealed a complete transcription unit composed of a cat box, a tata box, a ribosome-binding sequence, a polyadenylation signal and an open reading frame (orf) of 2940 bp transcribed from left to right. the amino acid (aa) sequence deduced from this orf corresponded to that of a protein with 979 aa and had the characteristic features of membrane gp including a 20-aa signal sequence a ... | 1990 | 1692002 |
| production and characterization of a human monoclonal antibody, reactive with a conserved epitope on gp41 of human immunodeficiency virus type i. | a human epstein-barr virus-transformed lymphoblastoid b-cell line was generated from peripheral blood mononuclear cells (pbmc) of an asymptomatic human immunodeficiency virus type i (hiv-1) seropositive donor, which produces a human monoclonal antibody k14 (igg1), reactive with an epitope on the transmembrane part (gp41) of the envelope glycoprotein of hiv-1. this monoclonal antibody reacts with a lysate of hiv-1-infected h9 cells, gradient purified hiv-1, and a vaccinia recombinant hiv-1 gp160 ... | 1990 | 1692724 |
| detection of free radical-induced dna damage with bromodeoxyuridine/hoechst flow cytometry: implications for bloom's syndrome. | the clinical radiosensitizer bromodeoxyuridine (brdu) was shown to enhance oxygen free radical-mediated growth inhibition. cells from bloom's syndrome, a rare autosomal recessive disorder characterized by pre- and post-natal growth deficits, telangiectatic erythema, recurrent respiratory infections and a high incidence of cancer, exhibit in culture a hypersensitivity to brdu. we analysed disturbed cell kinetics of bloom's syndrome fibroblasts and permanent b-cell lines with a novel cell kinetic ... | 1990 | 1692968 |
| morphological transformation of human keratinocytes expressing the lmp gene of epstein-barr virus. | the association of epstein-barr virus (ebv) with nasopharyngeal carcinoma (npc) has been known for some time, but the precise role of ebv in this cancer is poorly understood, due partly to the lack of an in vitro system for studying npc cells and the effect of ebv on epithelial cells. biopsies of npc tumours have revealed expression of the ebv latent membrane protein (lmp) in 65% of cases, suggesting that in at least some npc tumours lmp may contribute to cell transformation. here we address the ... | 1990 | 1692971 |
| a cell-to-cell mediated interaction involving monocytes and non-t/cd16+ natural killer (nk) cells is required for histamine h2-receptor-mediated nk-cell activation. | histamine (10(-4) to 10(-7) m) augmented natural killer cell cytotoxicity (nkcc) of human cd16+, non-t lymphocytes in vitro against the nk-sensitive target cells k562 erythroleukaemic, molt-4 lymphoma, chang liver cells and against epstein-barr virus (ebv)-transformed, nk-insensitive daudi b-lymphoblastoid target cells by a mechanism of action involving a prostaglandin- and interleukin 1 (il-1)-independent accessory function of monocytes. no evidence for the production of intermediary, nk-enhanc ... | 1990 | 1693229 |
| identification and characterization of a new surface membrane antigen found predominantly on malignant b lymphocytes. | a monoclonal antibody, 1d10, was derived that identifies a new antigenic epitope on the surface of malignant b lymphocytes. normal resting and stimulated lymphocytes do not express the antigen. the majority of individuals with acute epstein-barr virus infection express the antigen on their lymphocytes, and in these patients, the t lymphocyte may also be antigen positive. the antigen was found on b-lymphoid neoplasia from the early pre-b cell stage through terminally differentiated plasma cells, ... | 1990 | 1693529 |
| serum and salivary iga antibodies against a defined epitope of the epstein-barr virus nuclear antigen (ebna) are elevated in nasopharyngeal carcinoma. | the epstein-barr virus nuclear antigen i (ebna i) is the only latent ebv antigen consistently expressed in malignant tissues of the nasopharynx. a 20-amino-acid synthetic peptide, p107 contains a major epitope of ebna i. we tested sera from 210 patients with nasopharyngeal carcinoma (npc) and from 128 normal individuals (nhs) for iga antibodies to p107 using an enzyme-linked immunosorbent assay (elisa). whereas 191/210 (91%) of npc patients had iga antibodies to p107, only 17/128 (13.3%) of nhs ... | 1990 | 1693600 |
| selective virus inhibitors. | our recent efforts have been directed at the development of selective inhibitors of different classes of viruses, including adeno, pox, and herpesviruses [herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv)], (+/-)rna viruses (reo- and rotavirus), (-)rna viruses (influenza, parainfluenza, measles, respiratory syncytial, vesicular stomatitis and rabies virus) and retroviruses [i.e. human immunodeficiency virus (hiv), the ... | 1990 | 1693749 |
| identification of conserved and variant epitopes of human immunodeficiency virus type 1 (hiv-1) gp120 by human monoclonal antibodies produced by ebv-transformed cell lines. | using epstein-barr virus (ebv) transformation of b cells isolated from peripheral blood of two asymptomatic human immunodeficiency virus type 1-(hiv-1) infected subjects, we have produced four igg1 human monoclonal antibodies (hmabs) that bind to hiv-1 gp120, as determined by western blot analysis. two of these hmabs, designated n70-1.5e and n70-2.3a, react with epitopes of gp120 expressed by all strains tested thus far, and therefore, appear to identify conserved epitopes. the other two hmabs, ... | 1990 | 1694449 |
| expression of the bnlf-1 oncogene of epstein-barr virus in the skin of transgenic mice induces hyperplasia and aberrant expression of keratin 6. | the bnlf-1 gene of epstein-barr virus (ebv) encodes the latent membrane protein (lmp), one of the putative oncogene products of the virus. this gene has been expressed from two different enhancer-promoter constructs in transgenic mice, to determine its biological activity and possible contribution to oncogenesis. while transgenic mice expressing lmp in many tissues demonstrated poor viability, expression of lmp specifically in the epidermis induces a phenotype of hyperplastic dermatosis. concomi ... | 1990 | 1694724 |
| retroviral expression in mononuclear blood cells isolated from a patient with osteopetrosis (albers-schönberg disease). | we report the presence of reverse transcriptase activity in the supernatant of long-term culture of mononuclear blood cells (monocytes and lymphocytes) isolated from a 27-year-old patient suffering from benign osteopetrosis. the enzyme was purified to homogeneity according to the technique of chandra and steel, by chromatography, first on deae-cellulose (de 52) and then on phosphocellulose (p11). after purification, the enzyme was characterized biochemically for its template specificity and ioni ... | 1990 | 1695060 |
| hepatitis b surface antigen-specific cd8-positive t cell clones. antigen specificity and interleukin-2 production. | clones of hepatitis b surface antigen-reactive cd8+ and cd4+ t cells were obtained from peripheral blood mononuclear cells (pbm) of a hepatitis b immunized individual whose pbm proliferated when cultured with hepatitis b surface antigen (hbsag). lymphocytes were activated by culturing for 2 weeks with hbsag and high concentrations of interleukin-2 (il-2), then cloned in the presence of irradiated hbsag-activated pbm and autologous epstein-barr virus (ebv)-transformed b cells, together with antig ... | 1990 | 1695132 |
| an epstein-barr virus-specific cytotoxic t-cell epitope present on a- and b-type transformants. | in this report we describe a cytotoxic t-cell epitope in the epstein-barr virus nuclear antigen ebna 6. this epitope is present on both a- and b-type transformants. | 1990 | 1695259 |
| structural requirements for c3d,g/epstein-barr virus receptor (cr2/cd21) ligand binding, internalization, and viral infection. | the structure of cr2, the human c3d,g/ebv receptor (cr2/cd21) consists of 15 or 16 60-70 amino acid repeats called short consensus repeats (scrs) followed by a transmembrane and a 34-amino acid intracytoplasmic domain. functions of cr2 include binding the human complement component c3d,g when it is covalently attached to targets or cross-linked in the fluid phase. in addition, cr2 binds the epstein-barr virus (ebv) and mediates internalization of ebv and subsequent infection of cells. in order t ... | 1990 | 1695627 |
| cloned stromal cell lines derived from human whitlock/witte-type long-term bone marrow cultures. | we report a human bone marrow culture technique that initially parallels the murine whitlock/witte culture system. as in the murine system, b cells predominate over other cell types, and all differentiation stages from pre-b to plasma cell are observed. although these human long-term cultures pass through stages resembling phases i to iii of murine whitlock/witte cultures, no outgrowth of nonadherent cells was seen after cultures had reached the "crisis" phase unless epstein-barr virus (ebv)-tra ... | 1990 | 1696206 |
| suppression by protein kinase c inhibitors of tumor promoter enhancement of epstein-barr virus-induced growth transformation. | the tumor promoter teleocidin activates latent epstein-barr virus (ebv) genomes and enhances ebv-induced growth transformation of human b cells, with an activity comparable to that of 12-o-tetradecanoyl-phorbol-13-acetate (tpa). it has been suggested that the tpa induction of ebv genomes is mediated by protein kinase c (pkc), an enzyme closely linked to signal transduction. we examined newly isolated, highly selective pkc inhibitors, ucn-01 (7-hydroxyl-staurosporine) and calphostin c, for the po ... | 1990 | 1696445 |
| priming of cd4+ t cells specific for conserved regions of human immunodeficiency virus glycoprotein gp120 in humans immunized with a recombinant envelope protein. | a nonglycosylated denatured form of human immunodeficiency virus (hiv) 1 glycoprotein gp120 (env 2-3), which does not bind to cd4, was used with muramyl tripeptide as adjuvant to immunize hiv-seronegative healthy volunteers. in all the volunteers, three 50-micrograms injections of env 2-3 induced priming of cd4+ t cells specific for conserved regions of the native glycosylated gp120. moreover, we found that several major histocompatibility complex class ii (dr) alleles can function as restrictio ... | 1990 | 1696717 |
| evidence for diversity of plasmodium falciparum sporozoite surface antigens derived from analysis of antibodies elicited in humans. | we have compared the reactivities of antibodies developed by individuals frequently exposed to plasmodium falciparum infections with the epitopes contained within the repeats of the circumsporozoite (cs) protein and their reactivities with the epitopes of a native molecule(s) accessible on the sporozoite surface. results of direct-binding assays and competition assays between artificial and native molecules or between human antibodies and anti-cs monoclonal antibodies suggest that humans respond ... | 1990 | 1696936 |
| amylase-producing plasmacytoma cell lines, ad3 and fr4, with der(14)t(8;14) and dic(8)t(1;8) established from ascites. | we established two human plasma cell lines, fr4 and ad3, from ascitic fluid in a patient with iga k plasmacytoma (pc). aberrant amylase production was found in this patient. both ad3 and fr4 were free of epstein-barr virus, and both produced ig a k in vitro. they produced amylase of the salivary type in vitro. this was confirmed by the demonstration of amylase mrna comigrating with salivary gland mrna. these cell lines commonly had unusual chromosomal abnormalities der(14)t(8;14) and dic(8)t(1;8 ... | 1990 | 1697013 |
| oncogene expression and immunoglobulin synthesis in a north american burkitt (nab-2) lymphoma cell line with a 8;22 chromosome translocation. | a burkitt's lymphoma (bl) cell line, (nab-2) deriving from a north american patient, exhibited a 8;22 (q22;q11-12) translocation involving the c-myc gene and lambda immunoglobulin genes. in addition, nab-2 cells have two other consistent translocations: a 1;5 (p22;q23) and a 3;7 (p25;q22), with breakpoints close to the location of n-ras, fms, and raf-1 protooncogenes, respectively. in situ hybridization with myc, n-ras, and raf-1 radiolabeled dna probes to nab-2 chromosomes showed that none of t ... | 1990 | 1697668 |
| human monoclonal antibodies to pseudomonas aeruginosa type-specific lipopolysaccharides, toxin a and klebsiella capsular polysaccharides. | a number of human monoclonal antibodies (hmab) recognizing type-specific determinants expressed by the lipopolysaccharide (lps) of pseudomonas aeruginosa and by the capsular polysaccharide (cps) of klebsiella were generated for potential treatment of nosocomial infections. the goal is to administer these type-specific hmab prophylactically as a "cocktail" providing broad coverage. lymphoblastoid cell lines (lcl) secreting hmab recognizing p. aeruginosa lps, toxin a or klebsiella cps were obtaine ... | 1990 | 1698165 |
| epstein-barr virus infection in 59 orthotopic liver transplant patients. | fifty-nine orthotopic liver transplant (olt) patients were studied after transplantation to detect epstein-barr virus (ebv) primoinfection and reactivation. nineteen, all children under 10 years, were ebv seronegative. seroconversion occurred in 12 (63.3%) of the seronegative patients. most of these patients (10/12) seroconverted 2 or 3 months after transplantation; 11 out of the 12 demonstrated clinical signs at the time of seroconversion. from 9 primoinfected patients tested for ebv excretion, ... | 1990 | 1698249 |
| monoclonal autoantibodies from insulin-dependent diabetic patients: autoantibodies against beta-cell surface or cytoplasmic antigens. | to investigate the target antigen(s) recognized during the autoimmune process in insulin-dependent diabetes mellitus (iddm), we produced human monoclonal antibodies by epstein-barr virus transformation of peripheral blood lymphocytes from a large number (n = 50) of newly diagnosed iddm patients. screening by indirect immunofluorescence assay, using the rinm5f rat insulinoma cell line and eight other human or rat tumour cell lines, was performed to identify monoclonal antibodies that reacted with ... | 1990 | 1698582 |
| modulation of cellular gene expression in b lymphoma cells following in vitro infection by epstein-barr virus (ebv). | in vitro infection of ebv-negative lymphoma cell lines with immortalizing strains of epstein-barr virus induces the cell-surface expression of b-cell markers, such as the ebv receptor/cr2 (cd21) and the cd23 antigen. the non-immortalizing ebv variant, p3hri, which carries a deletion encompassing the ebv nuclear antigen 2 (ebna2) gene, fails to induce any such expression. we show here that the ebv-mediated up-regulation of cell-surface expression of these molecules is associated with an increased ... | 1990 | 1698730 |