| mouse x pig chimeric antibodies expressed in baculovirus retain the same properties of their parent antibodies. | the development of hybridoma and recombinant dna technologies has made it possible to use antibodies against cancer, autoimmune disorders, and infectious diseases in humans. these advances in therapy, as well as immunoprophylaxis, could also make it possible to use these technologies in agricultural species of economic importance such as pigs. porcine reproductive and respiratory syndrome virus (prrsv) is an arterivirus causing very important economic losses to the industry. passive transfer of ... | 2009 | 19301248 |
| molecular magnetic resonance imaging of myocardial perfusion with ep-3600, a collagen-specific contrast agent: initial feasibility study in a swine model. | cardiac magnetic resonance (mr) perfusion imaging during the first pass after intravenous administration of extracellular contrast agents is hampered by the spatial and temporal resolution achievable and by the artifacts seen in ultrafast mr imaging. furthermore, time-consuming quantitative data analysis is often added. the use of molecular mr imaging with a target-specific contrast agent with perfusion-dependent binding to myocardium may enable prolonged visualization of perfusion defects and t ... | 2009 | 19307474 |
| comparison of electrically induced flare response patterns in human and pig skin. | we compared the characteristics of neurogenic flare responses in human and pig skin to establish a translational research animal model. | 2009 | 19319479 |
| non-iterative variance component estimation in qtl analysis. | in variance component quantitative trait loci (qtl) analysis, a mixed model is used to detect the most likely chromosome position of a qtl. the putative qtl is included as a random effect and a method is needed to estimate the qtl variance. the standard estimation method used is an iterative method based on the restricted maximum likelihood (reml). in this paper, we present a novel non-iterative variance component estimation method. this method is based on henderson's method 3, but relaxes the c ... | 2009 | 19320767 |
| mitochondrial dna sequence variations in some italian wild boar populations. | in order to investigate the relationships between italian wild boar and major pig breeds, we studied the genetic variability of four wild boar populations in italy (arezzo, pisa, parma, bergamo) using a 533-bp fragment of the mitochondrial control region. sixty-nine wild boar samples were analysed, allowing the identification of 10 distinct haplotypes, which involve a total of 15 single nucleotide polymorphisms. phylogenetic and network analyses were performed also considering several sequences ... | 2009 | 19320773 |
| normal profiles for deciduous dental eruption in domestic piglets: effect of sow, litter, and piglet characteristics. | the deciduous dentition of the domestic pig is comprised of 28 teeth (2 x incisors 3/3, canine 1/1, premolars 3/3, molars 0/0). the timing and sequence of deciduous dental eruption were determined from oral exams on 233 yorkshire piglets from 0 to 5 wk of age. eruption occurred sooner in gilts for all molariform premolars (p(3), p(4), and p(4), p < 0.01) and first incisor, i(1) (p = 0.004). birth weight influenced eruption for all teeth except i(1) (i(1), p(3), p(3), p(4), and p(4); p < 0.01), w ... | 2009 | 19329477 |
| portal vein embolization with radiolabeled polyvinyl alcohol particles in a swine model: hepatic distribution and implications for pancreatic islet cell transplantation. | the distribution of radiolabeled polyvinyl alcohol microspheres (pvams) when infused into the portal vein of domestic swine was investigated, with the purpose of assessing implications for pancreatic islet cell transplantation. pvams measuring 100-300 microm (contour se) and labeled with (99m)tc were infused into the main portal vein of 12 swine, with intermittent portal venous pressure measurements. the infusion catheter was introduced antegradely via direct or indirect cannulation of the porta ... | 2009 | 19330373 |
| complex porcine model of atherosclerosis: induction of early coronary lesions after long-term hyperlipidemia without sustained hyperglycemia. | the incidence of coronary artery disease (cad) is still increasing in industrialized countries and it is even higher in diabetic patients. for experimental studies investigating the pathophysiology of cad, the use of an animal model comparable with the pathological situation in patients is crucial. | 2009 | 19340354 |
| evaluation of endoscopic-ultrasound-guided celiac ganglion irradiation with iodine-125 seeds: a pilot study in a porcine model. | implantation of iodine-125 ( (125)i) seeds under the guidance of endoscopic ultrasonography (eus) has been proved to be a safe alternative treatment option for advanced pancreatic cancer. the main purpose of this study was to evaluate the feasibility and safety of eus-guided radiation of the celiac ganglia with (125)i seeds in a porcine model. | 2009 | 19340740 |
| imaging sequences for intraoperative mr-guided laparoscopic liver resection in 1.0-t high field open mri. | the aim of this study was to identify suitable interactive (dynamic) magnetic resonance (mr) sequences for real-time mr-guided liver dissection in a 1.0-t high field open mri system. four dynamic sequences encompassing balanced steady state free precession (bssfp), t1w gradient echo (gre), t2w gre and t2w fast spin echo (fse) were analysed regarding the image quality, artefact susceptibility and the performance of snr and cnr. the t2w fse sequence (1.5 s/image) was considered superior because of ... | 2009 | 19350246 |
| modulation of porcine (sus scrofa domestica) and pheasant (phasianus colchicus) carbonyl reducing enzymes by anthelmintic therapy with flubendazole. | flubendazole (flu) is a widely administered benzimidazole anthelmintic indicated for the control of parasitic diseases in farm animals including pigs and pheasants. this study was designed to test the biotransformation of flu in control animals and animals treated with flu in recommended therapeutic doses. the activities of several pheasant and porcine hepatic and intestinal carbonyl reducing enzymes and their modulation by flu were also studied. twelve adult pheasant hens, approximately 1 year ... | 2008 | 19356067 |
| porcine cd18 mediates actinobacillus pleuropneumoniae apxiii species-specific toxicity. | actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, produces apx toxins that are recognized as major virulence factors. recently, we showed that apxiiia-cytotoxic activity specifically targets sus scrofa leukocytes. since both ltxa from aggregatibacter actinomycetem comitans (aggressive periodontitis in humans) and lkta from mannheimia haemolytica (pneumonia in ruminants) share this characteristic, respectively towards human and ruminant leukocytes, and because both ... | 2009 | 19356397 |
| intra-abdominal pressure development after different temporary abdominal closure techniques in a porcine model. | decompressive laparotomy followed by temporary abdominal closure (tac) is an established prophylaxis and treatment for abdominal compartment syndrome. the herein presented study aimed at the comparison of volume reserve capacity and development of intra-abdominal hypertension after forced primary abdominal closure and different tac techniques in a porcine model. | 2009 | 19359923 |
| jejunal pouch reconstruction but not preservation of duodenal passage after total gastrectomy reduces plasma cholecystokinin and pancreatic polypeptide long term in pigs. | the long-term effects of reconstructions of the gastrointestinal tract after gastrectomy on plasma levels of gastrointestinal hormones that contribute to food intake controls were evaluated. | 2009 | 19368131 |
| total gastrectomy causes a sustained, long-term elevation of somatostatin in plasma, independent of the mode of reconstruction in pigs. | the long-term effects of gastrectomy and various reconstructions of the gastrointestinal tract on fasting plasma levels of gastrointestinal hormones known to contribute to the control of gastrointestinal motor function were evaluated in pigs. | 2009 | 19368132 |
| multipolar radiofrequency ablation in controlling hemorrhage from blunt liver trauma. | the aim of this study is to investigate the use of multipolar radiofrequency (rf) ablation with internally cooled electrodes to control hemorrhage percutaneously from blunt liver trauma. | 2009 | 19371528 |
| video-assisted lung resection using radiofrequency ablation in a porcine model. | the aim of this study was to assess the feasibility, efficacy, and safety of video-assisted lingula resection using radiofrequency ablation (rfa) with internally cooled electrodes in a porcine model. | 2009 | 19376530 |
| distribution of cocaine- and amphetamine-regulated transcript in the hippocampal formation of the guinea pig and domestic pig. | this study provides a detailed description concerning the distribution of cocaineand amphetamine-regulated transcript (cart) subunits - cart(61-102) and rhcart(28-116) - in the hippocampal formation (hf) of the guinea pig and domestic pig, focussing on the dentate gyrus (dg) and hippocampus proper (hp). although in both studied species cart-immunoreactive (cart-ir) neuronal somata and processes were present generally in the same layers, some species-specific differences were still found. in the ... | 2009 | 19384826 |
| [(18)f]fluoroacetate is not a functional analogue of [(11)c]acetate in normal physiology. | [(11)c]acetate (c-ac) is a general pet tracer of cellular carbon flux and useful for clinical imaging in heart disease as well as prostate cancer and other tumours. c-ac has a high (70%) whole-body extraction fraction, proportional to blood flow in many organs. trapping is related to organ-specific enzymatic activation and formation of [(11)c]-acetyl-coa, the fate of which has been well characterized. due to the logistic challenges with c-ac, 2-[(18)f]fluoroacetate (f-ac) has been proposed as a ... | 2009 | 19387639 |
| effect of recipient breed on delivery rate of cloned miniature pig. | the miniature pig is regarded as a better organ donor breed for xenotransplantation than other pig breeds because the size of their organs is similar to that of humans. to improve efficiency of cloned miniature pig production, we analysed the effect of breed difference between donor cells and embryo recipients on pregnancy rate and delivery rate. cloned porcine embryos derived from domestic or miniature pig donor cells were transferred to domestic or miniature recipient pigs. delivery rate was s ... | 2009 | 19393119 |
| occurrence and pathways of manure-borne 17beta-estradiol in vadose zone water. | the hormone 17beta-estradiol (e2) can cause endocrine disruption in sensitive species at part per trillion concentrations. the persistence and transport pathways of manure-borne e2 in agricultural soils were determined by comparing its occurrence with the transfer of water and the transport of non-sorbing fluorobenzoic acid (fba) tracers. this comparison was done using capillary wick lysimeters installed 0.61m beneath three corn (zea mays l.) plots that receive swine (sus scrofa domesticus) manu ... | 2009 | 19394677 |
| feral swine contact with domestic swine: a serologic survey and assessment of potential for disease transmission. | feral swine (sus scrofa) are present in 38 of the 50 united states, and their populations continue to expand. domestic swine are widely regarded as vulnerable to diseases harbored by feral swine. our objectives were to determine antibody prevalence for selected pathogens in texas feral swine populations and identify contact events between feral and domestic swine. overall prevalence of antibodies against brucellosis and pseudorabies virus was 11% and 30%, respectively. antibodies to porcine repr ... | 2009 | 19395751 |
| influence of sex on the meiotic segregation of a t(13;17) robertsonian translocation: a case study in the pig. | comparison of male versus female meiotic segregation patterns for robertsonian translocation (rt) carriers with similar genetic background has rarely been reported in mammalian species. | 2009 | 19403564 |
| porcine hypothalamic aromatase cytochrome p450: isoform characterization, sex-dependent activity, regional expression, and regulation by enzyme inhibition in neonatal boars. | domestic pigs have three cyp19 genes encoding functional paralogues of the enzyme aromatase cytochrome p450 (p450arom) that are expressed in the gonads, placenta, and preimplantation blastocyst. all catalyze estrogen synthesis, but the gonadal-type enzyme is unique in also synthesizing a nonaromatizable biopotent testosterone metabolite, 1oh-testosterone (1oh-t). p450arom is expressed in the vertebrate brain, is higher in males than females, but has not been investigated in pigs, to our knowledg ... | 2009 | 19403926 |
| pre-natal stress amplifies the immediate behavioural responses to acute pain in piglets. | pre-natal stress (pns) or undernutrition can have numerous effects on an individual's biology throughout their lifetime. some of these effects may be adaptive by allowing individuals to tailor their phenotype to environmental conditions. here we investigated, in the domestic pig sus scrofa, whether one possible consequence of a predicted adverse environment could be altered pain perception. the behavioural response of piglets to the surgical amputation ('docking') of their tail or a sham procedu ... | 2009 | 19411272 |
| relaxin and maternal lactocrine programming of neonatal uterine development. | in mammals, including the pig (sus scrofa domesticus), structural patterning and functional programming of uterine tissues involve events that occur shortly after birth. porcine endometrial development between birth (postnatal day 0 [pnd 0]) and pnd 15 is estrogen receptor (er) dependent and estrogen sensitive. the endometrium is relaxin (rlx) receptor (rxfp1) positive and eralpha negative at birth. uterine expression of rxfp1 and eralpha, detectable by pnd 2, increases with age from pnd 0 to 14 ... | 2009 | 19416179 |
| recombinant antigen targets for serodiagnosis of african swine fever. | african swine fever (asf) is an infectious and economically important disease of domestic pigs. there is no vaccine, and so reliable diagnosis is essential for control strategies. the performance of four recombinant asf virus (asfv) protein (pk205r, pb602l, p104r, and p54)-based enzyme-linked immunosorbent assays (elisas) was evaluated with european porcine field sera that had been established by office international des epizooties (oie)-approved tests to be asfv negative (n = 119) and asfv posi ... | 2009 | 19420186 |
| cadaveric volatile organic compounds released by decaying pig carcasses (sus domesticus l.) in different biotopes. | forensic entomology uses pig carcasses to surrogate human decomposition and to investigate the entomofaunal colonization. insects communicate with their environment through the use of chemical mediators, which in the case of necrophagous insects, may consist in the cadaveric volatile organic compounds (vocs) released by the corpse under decomposition. previous studies have focused on cadaveric vocs released from human corpses. nevertheless, studies on human corpses are restricted for many reason ... | 2009 | 19423246 |
| pure 'natural orifice transluminal endoscopic surgery' for transvaginal nephrectomy in the porcine model. | to determine the technical feasibility and reproducibility of pure natural orifice transluminal endoscopic surgery (notes) transvaginal nephrectomy using notes-specific instrumentation, with no transabdominal assistance. | 2009 | 19426194 |
| an integrated rh map of porcine chromosome 10. | whole genome radiation hybrid (wg-rh) maps serve as "scaffolds" to significantly improve the orientation of small bacterial artificial chromosome (bac) contigs, order genes within the contigs and assist assembly of a sequence-ready map for virtually any species. here, we report the construction of a porcine: human comparative map for pig (sus scrofa) chromosome 10 (ssc10) using the imnprh2(12,000-rad) porcine wg-rh panel, integrated with the imprh(7000-rad) wg-rh, genetic and bac fingerprinted c ... | 2009 | 19426492 |
| detection of hepatitis e virus in wild boars of rural and urban regions in germany and whole genome characterization of an endemic strain. | hepatitis e is an increasingly diagnosed human disease in central europe. besides domestic pigs, in which hepatitis e virus (hev) infection is highly prevalent, wild boars have been identified as a possible source of human infection. in order to assess the distribution of hev in the wild boar population of germany, we tested liver samples originating from different geographical regions for the presence of the hev genome and compared the detected sequences to animal and human hev strains. | 2009 | 19442307 |
| endemic hepatitis e in two nordic countries. | antibodies against hepatitis e virus (anti-hev) were found in 248 swedish and danish patients between 1993 and 2007. most patients were symptomatic and tested for anti-hev due to travel abroad. among patients with known country of infection, most were infected in asia, mainly on the indian subcontinent. however, 29 patients were infected in europe, nine of these had hev igm and/or hev rna in serum. in sera from 65 of 141 tested patients hev rna could be detected, and 63 strains could be typed by ... | 2009 | 19442399 |
| recording diffusion responses from contralateral intercostal muscles after stimulus-triggered electromyography: refining a tool for the assessment of thoracic pedicle screw placement in an experimental porcine model. | a new stimulus-triggered electromyography (emg) test for detecting stimulus diffusion to contralateral intercostal muscles during thoracic pedicle screw placement was assessed in a porcine model. | 2009 | 19444052 |
| comparison of vascularized osteoperiosteal femur flaps and nonvascularized femur grafts for reconstruction of mandibular defects: an experimental study. | the aim of this study was to compare the vascularized osteoperiosteal femur flaps (vffs) and the nonvascularized femur grafts (nvfgs) for reconstruction of the mandibular defects of pigs. | 2009 | 19446201 |
| wound healing and the immune response in swine treated with a hemostatic bandage composed of salmon thrombin and fibrinogen. | we investigated the inflammatory response in pigs exposed to salmon fibrinogen/thrombin dressings. animals were exposed to the material in 3 ways: (a) thrombin and fibrinogen were injected intravenously, (b) dual full-thickness skin lesions were surgically created on the dorsal aspect of the swine and treated with the fibrinogen/thrombin bandage and a commercial bandage or (c) a fibrinogen/thrombin bandage was inserted through an abdominal incision into the peritoneal cavity. blood was collected ... | 2009 | 19449202 |
| prevention of vasa vasorum neovascularization attenuates early neointima formation in experimental hypercholesterolemia. | vasa vasorum (vv) neovascularization is a key feature of early atherosclerosis and adds substantial endothelial exchange-surface to the coronary vessel wall. thus, it is conceivable that vv neovascularization favors the entry of pro-inflammatory and pro-atherosclerotic blood components into the coronary vessel wall. we sought to investigate the effects of thalidomide (th), a potent anti-angiogenic drug on vasa vasorum (vv) neovascularization, vessel wall inflammation, and neointima formation in ... | 2009 | 19458984 |
| molecular taxonomy, phylogeny and biogeography of nematodes belonging to the trichinella genus. | studying parasites of the genus trichinella provides scientists of today many advantages. this is a group of zoonotic nematodes that circulates freely among wildlife hosts with one in particular, trichinella spiralis that is exceptionally well adapted to domestic swine. recent reports suggest that human infections from hunted animals are on the rise worldwide and numerous countries still experience problems with t. spiralis in their domestic food supplies. trichinella is a genus whose members ar ... | 2009 | 19460327 |
| meat juice as diagnostic sample for virological and serological diagnosis of classical swine fever. | the objective of this paper was to assess if meat juice is a suitable substrate for virological and serological diagnosis of classical swine fever (csf). fifty-six domestic pigs and 21 wild boars experimentally vaccinated and/or infected as well as 129 field samples from wild boars were involved in this study. meat juice from diaphragm, forequarter and hindquarter was used for investigations. csfv and viral rna were detected in meat juice between days 5 and 21 post infection (pi). animals which ... | 2009 | 19462640 |
| imaging of the midpalatal suture in a porcine model: flat-panel volume computed tomography compared with multislice computed tomography. | an investigation was conducted to compare the image quality of prototype flat-panel volume computed tomography (fpvct) and multislice computed tomography (msct) of suture structures. | 2009 | 19464211 |
| comparative analysis of solar radiation-induced cellular damage between ex vivo porcine skin organ culture and in vitro reconstructed human epidermis. | reconstructed human epidermis models (rhe) constitute an innovative alternative to study phototoxicity and photoprotection in the cosmetic industry. however, little information is currently available concerning the harmful effects of solar-simulated radiation (ssr) in these in vitro skin models. in this study, the phototoxic effects of a single acute ssr dose of 275 kj m(-2) were evaluated in a validated rhe model (from skinethic), and were compared with those obtained from an ex vivo skin organ ... | 2009 | 19467030 |
| trichuris suis and trichuris trichiura are different nematode species. | in this paper, a morphological and biometrical study by optical microscopy and scanning electronic microscopy (sem) of trichuris suis isolated from different hosts (sus scrofa domestica and sus scrofa scrofa) and trichuris trichiura isolated from chimpanzee, has been carried out. our results demonstrate the existence of typical pericloacal papillae in both species. biometrical parameters of t. suis and t. trichiura overlapped but males and females of t. trichiura tended to be shorter and thinner ... | 2009 | 19467214 |
| usefulness of mri to differentiate between temporary and long-term coronary artery occlusion in a minimally invasive model of experimental myocardial infarction. | the surgical technique employed to determine an experimental ischemic damage is a major factor in the subsequent process of myocardial scar development. we set out to establish a minimally invasive porcine model of myocardial infarction using cardiac contrast-enhanced magnetic resonance imaging (ce-mri) as the basic diagnostic tool. twenty-seven domestic pigs were randomized to either temporary or permanent occlusion of the left anterior descending artery (lad). temporary occlusion was achieved ... | 2009 | 19472001 |
| receptor activator of nuclear factor kappa b ligand in an experimental intervertebral disc degeneration. | this study was designed to clarify the role of the receptor activator of nuclear factor kappa b ligand (rankl) in the process of discus degeneration and spondylarthrosis. it was hypothesized that experimental discus lesion would initiate not only local bone remodelling but also increased osteoclast formation on a location remote to the injury site due to altered spinal biomechanics. it was speculated that these changes in vertebral bone remodelling could be reflected in an increased rankl expres ... | 2009 | 19473572 |
| isolation and fluorescence spectrum of cardiac myosin from pig heart. | cardiac myosin is the most abundant and bioactive protein presented in heart tissue. in this work, a simple method was described for preparation of high purity cardiac myosin for further research. with the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.5 mg myosin per gram of wet tissue with a specific activity of 0.293 u/mg. the purification factor was 4.9-fold with an activity recovery of 42.1%. in addition, the investigation of fluorescence spectrum shown t ... | 2009 | 19475499 |
| chromosomal assignment of porcine pregnancy-associated glycoprotein gene family. | this study presents the chromosomal assignment of a multiple pregnancy-associated glycoprotein (pag) gene family in the domestic pig (ppag). the ppag locus was identified by physical mapping (fluorescent in situ hybridisation-fish; with various probes), and additionally confirmed by southern hybridisation of ppag amplicons using laser microdissected sus scrofa chromosome 1 (ssc1), as genomic templates. various ppag probes were produced with the use of diverse identified templates: ppag1-6, -8, - ... | 2010 | 19487088 |
| endothelialization of nonapposed stent struts located over the origin of a side branch: results with different carbofilm-coated stents. | to evaluate the degree of endothelialization of the nonapposed struts located at the ostia of side branches. | 2009 | 19490356 |
| drug delivery at the aortic valve tissues of healthy domestic pigs with a paclitaxel-eluting valvuloplasty balloon. | restenosis occurs invariably within 1 year following balloon valvulopasty in aortic valve stenosis. the mechanism of restenosis seems to involve a dynamic cellular component that could be a target for drug inhibition. we investigated the feasibility of local drug delivery at the aortic valve tissues of healthy pigs with a paclitaxel-eluting balloon. | 2009 | 19490359 |
| closed-chest experimental porcine model of acute myocardial infarction-reperfusion. | progress in cardiovascular regenerative medicine research requires the availability of appropriate experimental animal models that are as close to humans as feasible. our objective was to assess the validity of a porcine endovascular model of myocardial infarction and reperfusion. | 2009 | 19490947 |
| enhanced external counterpulsation promotes growth cytokines-mediated myocardial angiogenesis in a porcine model of hypercholesterolemia. | enhanced external counterpulsation (eecp) improves ischemia in patients with refractory angina pectoris, but the mechanism remains unclear. to explore the mechanisms of eecp action, we detected progenitor cells presenting any of the following markers cd34(+), cd29(+), and cd106(+). | 2009 | 19493469 |
| a comparison of kidney oxygenation profiles between partial and complete renal artery clamping during nephron sparing surgery in a porcine model. | to compare kidney oxygenation profiles between partial and complete renal artery clamping during nephron sparing surgery (nss) in a porcine model. | 2009 | 19497169 |
| classification of worldwide bovine tuberculosis risk factors in cattle: a stratified approach. | the worldwide status of bovine tuberculosis (btb) as a zoonosis remains of great concern. this article reviews the main risk factors for btb in cattle based on a three-level classification: animal, herd and region/country level. a distinction is also made, whenever possible, between situations in developed and developing countries as the difference of context might have consequences in terms of risk of btb. recommendations are suggested to animal health professionals and scientists directly invo ... | 2009 | 19497258 |
| first report of bvdv circulation in sheep in argentina. | pestiviruses are capable of infecting a wide range of animals within the order artyodactila. currently, the genus pestivirus includes bovine viral diarrhea virus 1 (bvdv-1) and 2 (bvdv-2), border disease virus (bdv), and classical swine fever virus (csfv). bvdv-1, bvdv-2 and bdv are able to cross species barrier to infect a wide range of hosts, whereas csfv is restricted to domestic pigs and wild boars. in argentina, 70% of cattle are seropositive to bvdv. although there were some serological st ... | 2009 | 19501923 |
| lipid emulsion combined with epinephrine and vasopressin does not improve survival in a swine model of bupivacaine-induced cardiac arrest. | this study sought to evaluate the efficacy of lipid emulsion in reversing bupivacaine-induced cardiovascular collapse when added to a resuscitation protocol that included the use of epinephrine and vasopressin. | 2009 | 19512878 |
| selective versus nonselective arterial clamping during laparoscopic partial nephrectomy: impact upon renal function in the setting of a solitary kidney in a porcine model. | laparoscopic partial nephrectomy has emerged as a standard of care for small renal masses. nevertheless, there remains concern over the potential for irreversible insult to the kidney as a result of exposure to warm ischemia. we aim to investigate the utility of selective segmental arterial clamping as a means to reduce the potential for ischemic damage to a solitary kidney during laparoscopic partial nephrectomy utilizing a porcine model. | 2009 | 19514812 |
| toxoplasma gondii infection in wild boars (sus scrofa) bred in brazil. | domestic pigs are considered to be important sources of toxoplasma gondii infection for humans. due to the increased consumption of wild boar meat in brazil, this species may also be an important source of the parasite. the objective of the present study was to detect t. gondii infection in 306 blood samples collected from wild boars bred in the state of são paulo, brazil. samples were analyzed using the modified agglutination test (mat), and 14 (4.5%) of them yielded positive results. modern br ... | 2009 | 19515494 |
| association of intramyocardial high energy phosphate concentrations with quantitative measures of the ventricular fibrillation electrocardiogram waveform. | quantitative measures of the ventricular fibrillation (vf) electrocardiogram (ecg) have been correlated with the success of rescue shocks, making them ideal measures for guiding resuscitative interventions. correlation of intramyocardial energy stores with the change in quantitative vf ecg measures would provide mechanistic insight into their utility. we sought to investigate the relationship between intramyocardial energy stores and four quantitative ecg measures. | 2009 | 19523740 |
| from wild animals to domestic pets, an evolutionary view of domestication. | artificial selection is the selection of advantageous natural variation for human ends and is the mechanism by which most domestic species evolved. most domesticates have their origin in one of a few historic centers of domestication as farm animals. two notable exceptions are cats and dogs. wolf domestication was initiated late in the mesolithic when humans were nomadic hunter-gatherers. those wolves less afraid of humans scavenged nomadic hunting camps and over time developed utility, initiall ... | 2009 | 19528637 |
| endoscopic closure of large colonic perforations using an over-the-scope clip: a randomized controlled porcine study. | perforation of the colon is considered to be one of the most serious complications of flexible endoscopy. the over-the-scope clip system (otsc) has previously been shown to close small colonic perforations effectively. the aim of this randomized controlled porcine study was to compare acute closure strengths between the novel 11-mm over-the-scope-clip and surgical closure for large colonic perforations. | 2009 | 19533550 |
| animal farm: pig in the middle. | | 2009 | 19536212 |
| characterization of the replication-competent porcine endogenous retrovirus class b molecular clone originated from korean domestic pig. | xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. the porcine endogenous retrovirus (perv) cannot be eliminated because of its presence in the germline dna. three subgroups of the replication-competent perv (perv-a, perv-b, and perv-c) have been identified in pigs. we constructed a molecular clone of perv-b from a korean domestic pig bac clone containing perv genomes, and its replication competency was characterized in human cells. the pol region of per ... | 2009 | 19543822 |
| investigation of blood typing method for seoul national university miniature pig. | pig blood group antigens may be present on grafted tissue as 16 isoforms, including the major one, a substrance. seoul national university (snu) miniature pigs and domestic pigs were used in this study. polymerase chain reaction (pcr) was performed for the erythrocyte antigen a (eaa) gene, and reverse transcriptase-pcr for pig a transferase fucosyl transferase (fut) 1 and fut-2. the hemagglutination test was performed with murine monoclonal anti-a and anti-b antibodies (mab), and immunohistochem ... | 2009 | 19545757 |
| cell-cell adhesion in the prelaminar region of the optic nerve head: a possible target for ionic stress. | this paper aims to study the anterior surface of the optic nerve in relation to its ability to support a source of stress acting from the vitreous cavity. the intercellular junctions of the lining astrocytes mediated by cellular adhesion molecules (cams) may be the main targets for ionic stress. | 2009 | 19546602 |
| natural orifice transluminal endoscopic surgery gastrotomy closure with an over-the-endoscope clip: a randomized, controlled porcine study (with videos). | secure endoscopic closure of transgastric natural orifice transluminal endoscopic surgery (notes) access is of paramount importance. the over-the-scope clip (otsc) system has previously been shown to be effective for notes gastrotomy closure. | 2009 | 19559416 |
| effect of natural or vaccine-induced porcine circovirus type 2 (pcv2) immunity on fetal infection after artificial insemination with pcv2 spiked semen. | the objectives of this study were to determine if vaccination against porcine circovirus type 2 (pcv2) or previous pcv2 infection of the dam are sufficient to prevent fetal infection when dams are artificially inseminated with pcv2-spiked semen. nine sows (sus domestica) were allocated into three groups of three dams each: the pcv2 naïve negative control group 1 was artificially inseminated with extended pcv2 dna negative semen during estrus, whereas the extended semen used in the vaccinated gro ... | 2009 | 19559470 |
| comparison of inflammatory response after implantation of sirolimus- and paclitaxel-eluting stents in porcine coronary arteries. | although both sirolimus (cypher) and paclitaxel (taxus) drug-eluting stents have demonstrated efficacy and safety in clinical trials, human autopsy data have raised concerns about long-term healing and the potential for local inflammatory reactions. | 2009 | 19564562 |
| the effect of anterior spinal fusion on spinal canal development in an immature porcine model. | experimental study. | 2009 | 19564754 |
| experimental comparison of the stapled intestinal anastomotic techniques. | various techniques of stapled intestinal anastomoses are performed in gastroenterological surgery. little is known about which technique is optimal. the intestines of a domestic pig were used in this study. stapled intestinal anastomoses of three types-functional end-to-end anastomosis (fetea), stapled side-to-end anastomosis (stea), and stapled end-to-end anastomosis (eea)-were constructed using pig intestines. the times for constructing anastomoses, length of the completed anastomosed intestin ... | 2009 | 19579195 |
| discovery of swine as a host for the reston ebolavirus. | since the discovery of the marburg and ebola species of filovirus, seemingly random, sporadic fatal outbreaks of disease in humans and nonhuman primates have given impetus to identification of host tropisms and potential reservoirs. domestic swine in the philippines, experiencing unusually severe outbreaks of porcine reproductive and respiratory disease syndrome, have now been discovered to host reston ebolavirus (rebov). although rebov is the only member of filoviridae that has not been associa ... | 2009 | 19590002 |
| global left ventricular function: assessment with dual-source ct versus conventional ventriculography in a porcine model. | cardiac computed tomography (ct) and conventional biplane ventriculography are established methods for the determination of ventricular function. recently dual source ct was introduced for cardiac ct scanning providing a temporal resolution of 83 ms independent of the patient's heart rate. the purpose of this study was to assess the global left ventricular function using retrospectively ecg-gated dual source computed tomography (dsct) in comparison to conventional ventriculography. | 2009 | 19593940 |
| the degree of resistance to freezing-thawing is related to specific changes in the structures of motile sperm subpopulations and mitochondrial activity in boar spermatozoa. | the main aim of this work was to analyze the possible relationship between the structures of motile-sperm subpopulations and boar (sus scrofa domesticus) sperm resistance to freezability. for this purpose, 45 boar ejaculates were subjected to a standard freezing-thawing protocol, and afterwards they were classified into three groups, in accordance with their resistance to freezing-thawing. our analysis yielded four separate motile-sperm subpopulations in all of the studied ejaculates, both in fr ... | 2009 | 19604570 |
| comparison of efficacy of pulsed biphasic waveform and rectilinear biphasic waveform in a short ventricular fibrillation pig model. | the waveform designs and their relative defibrillation efficacy of external biphasic waveforms may differ remarkably among manufacturers. in this study, we compared pulsed biphasic waveform (pbw) with rectilinear biphasic waveform (rbw) and their effects on terminating ventricular fibrillation (vf). | 2009 | 19604618 |
| pro-inflammatory, pleiotropic, and anti-inflammatory tnf-alpha, il-6, and il-10 in experimental porcine intervertebral disk degeneration. | the aim of this study was to check the balance between tumor necrosis factor-alpha (tnf-alpha), interleukin-6 (il-6), and interleukin-10 (il-10) in well-developed end-stage disk disease in the disk itself as well as in paradiskal spine. in 6 domestic pigs the cranial bony end plate of the l4 vertebra was perforated to the nucleus pulposus. at 3 months the degenerated experimental and contiguous control disks, together with the adjoining bony and cartilaginous vertebral end plates, bone marrow, a ... | 2009 | 19605905 |
| first record of pseudorabies in feral swine in nebraska. | in 2007, two new populations of feral swine were discovered in nance and valley counties, nebraska, usa. necropsies and serologic testing was done on two individuals from the nance county herd. results indicated that a lactating sow had positive antibodies for pseudorabies virus (prv). investigations conducted by nebraska game and parks commission law enforcement division confirmed that the infected individual was transported illegally to nebraska, usa, from texas, usa. all domestic swine herds ... | 2009 | 19617504 |
| transforming growth factor-alpha in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure. | we examined the effects of transforming growth factor-alpha (tgf-alpha) to develop a defined medium for in vitro maturation (ivm) of porcine (sus scrofa domesticus) oocytes. cumulus-oocyte complexes (cocs) were matured in porcine oocyte medium containing 3mg/ml polyvinyl alcohol (pom) and tgf-alpha (0, 1, 10, or 100 ng/ml) in the presence or absence of the gonadotropins equine chorionic gonadotropin (ecg) and human chorionic gonadotropin (hcg). in the absence of gonadotropins, adding 10 ng/ml tg ... | 2009 | 19632712 |
| transplantation of bone marrow-derived stem cells improves myocardial diastolic function: strain rate imaging in a model of hibernating myocardium. | the aim of this study was to evaluate the cardioprotective effects of bone marrow-derived stem cells on myocardial compliance in a chronic ischemia model regarding strain rate (sr) parameters during dobutamine stress echocardiography (dse). | 2009 | 19647407 |
| preferences of minipigs for environmental enrichment objects. | the minipig is an increasingly popular species for use in behavioral and toxicologic studies. as a result, quantification of environmental enrichment preferences for this species is especially important. we exposed 6 individually housed prepubertal female yucatan minipigs to 1 of 3 different objects on a rotating schedule: 2 sessions with a hard plastic ball (diameter, 21.0 cm), and 3 sessions each with a large plastic apple (diameter, 22.5 cm) and a soft rubber cone (height, 48.0 cm). objects w ... | 2009 | 19653948 |
| african swine fever: how can global spread be prevented? | african swine fever (asf) is a devastating haemorrhagic fever of pigs with mortality rates approaching 100 per cent. it causes major economic losses, threatens food security and limits pig production in affected countries. asf is caused by a large dna virus, african swine fever virus (asfv). there is no vaccine against asfv and this limits the options for disease control. asf has been confined mainly to sub-saharan africa, where it is maintained in a sylvatic cycle and/or among domestic pigs. wi ... | 2009 | 19687038 |
| healing of mid-dermal burns in a diabetic porcine model. | wound healing is delayed in diabetic patients. we developed a diabetic-porcine burn model and compared the healing of partial-thickness burns in normal and diabetic pigs. we hypothesized that wound healing would be delayed in the diabetic swine. diabetes mellitus was chemically induced in three domestic pigs (25-50 kg) by intravenous injection of streptozotocin 130 mg/kg over 30 minutes. glucose levels were maintained between 250 and 500 mg/dl by injecting short-acting or long-acting insulin 1 u ... | 2009 | 19692921 |
| frequent transmission of hepatitis e virus among piglets in farms in southern france. | the present study aimed to assess whether hepatitis e virus (hev) is present in domestic pigs in southern france, and to determine the relationship between hev sequences detected from pigs and from humans. two hundred fifteen sera, 207 stools, and 107 bile samples were collected from 3- or 6-month-old pigs from different regions of southern france. pig igg anti-hev antibodies testing was performed using a commercial elisa kit with minor modifications. pig hev rna was tested by real-time pcr and ... | 2009 | 19697419 |
| initial in vivo studies with a polymer-based mr-compatible guide wire. | the purpose of this study was to evaluate a new polymer-based and magnetic resonance (mr) imaging-compatible guide wire for its application in mr-guided endovascular interventions, particularly for interventional peripheral mr angiography in swine experiments in vivo. a passive device tracking method tailored to the specific conditions of peripheral mr angiography was developed. near-real-time visualization of the guide wire was accomplished in vivo in the carotid artery, aorta, heart, and iliac ... | 2009 | 19699660 |
| anti-inflammatory effects of c1-inhibitor in porcine and human whole blood are independent of its protease inhibition activity. | c1-inhibitor (c1-inh) is an important biological inhibitor, regulating several protein cascade systems. recent research has shown that the molecule exhibits properties not dependent on its protease inhibition activity. serum and whole blood from pigs and humans were pre-incubated with c1-inh, ic1-inh or the complement inhibitors spice or compstatin. whole, live escherichia coli were then added for further incubation. complement activation, a range of cytokines, chemokines and growth factors, as ... | 2010 | 19710096 |
| arginine-vasopressin attenuates beneficial norepinephrine effect on jejunal mucosal tissue oxygenation during endotoxinaemia. | the objective of the present study was to investigate the effects of increasing doses of norepinephrine (ne) with or without arginine-vasopressin (avp) on intestinal oxygen supply and jejunal mucosal tissue oxygen tension in an acute endotoxic pig model. | 2009 | 19720610 |
| amphiregulin promotes the proliferation of trophoblast cells during preimplantation development of porcine embryos. | our objective was to investigate the effects of in vitro culture (ivc) medium supplemented with amphiregulin (areg) on the preimplantation embryonic development of porcine (genus: sus domestica, species: landrace) embryos derived from in vitro fertilization (ivf) and parthenogenetic activation (pa). in vitro fertilization and pa embryos at the 1-cell stage were cultured in ivc medium supplemented with 0, 0.5, 5, or 50 ng/ml areg for 7 d. there were significantly greater total numbers of cells in ... | 2009 | 19729194 |
| distant effects of nitric oxide inhalation in endotoxemic pigs. | inhalation of nitric oxide (ino) has distant effects. by a blood- borne factor, ino down-regulates endogenous nitric oxide production in healthy pig lungs, resulting in vasoconstriction in lung regions not directly reached by ino. the aim of this study was to investigate whether ino has distant effects in endotoxemic pig lungs. the hypothesis was that ino down-regulates endogenous no production in lung regions not reached by ino. | 2010 | 19730256 |
| influence of habitat and seasonal variation on wild mammal diversity and distribution with special reference to the trypanosoma brucei gambiense host-reservoir in bipindi (cameroon). | to evaluate the role of wildlife in the resurgence and perenisation of human african trypanosomiasis (hat), we investigated the influence of habitat and seasonal variations on the diversity and spatial distribution of wild mammals, with special reference to those recognised as potential host-reservoirs of trypanosoma brucei gambiense in bipindi (southwestern cameroon). to achieve this, we carried out transect surveys in four habitat types over two years. a total of 31 mammal species were recorde ... | 2009 | 19732737 |
| the use of a hypo-osmotic swelling (hos) test on sperm of the pig (sus scrofa domesticus), emu (dromaius novaehollandiae), asian elephant (elephas maximus), hamadryas baboon (papio hamadryas hamadryas), and central rock rat (zyzomys pedunculatus). | a hypo-osmotic swelling test using talp-hepes medium over a range of 50 to 300 mosm/kg was applied to sperm from domestic and endangered species. maximal responses of curling of the sperm tails were seen over a range of osmolalities for epididymal sperm from the pig (100 mosm/kg), hamadryas baboon (range 50-125 mosm/kg), and central rock rat (range 50-100 mosm/kg), and the ejaculated sperm from the emu (50 mosm/kg) and the asian elephant (range 75-150 mosm/kg). a solution of talp-hepes medium at ... | 2009 | 19734955 |
| purification and characterisation of a p-selectin-binding molecule from the salivary glands of ornithodoros moubata that induces protective anti-tick immune responses in pigs. | ornithodoros moubata is an argasid tick that lives in africa in wild and synanthropic habitats. it feeds on warthogs, domestic swine and humans, and is able to transmit severe diseases such as human relapsing fever and african swine fever. the elimination of o. moubata from the synanthropic surroundings should improve control of the diseases it transmits. previous attempts to develop a vaccine against o. moubata showed that a salivary gland extract (sge) induced a protective response that inhibi ... | 2010 | 19735664 |
| differing sperm ability to penetrate the oocyte in vivo and in vitro as revealed using colloidal preparations. | the penetration ability of boar (sus scrofa domestica) spermatozoa exposed to viscous preparations under in vivo and in vitro fertilization conditions has been examined. experiments involving induced ovulation in prepubertal animals and surgical insemination directly into the oviduct isthmus revealed an advantage of colloidal preparations. based on within-animal comparisons, the incidence of penetration was 100% using both spermatozoa suspended in a viscous preparation of plant extracts and sper ... | 2009 | 19744704 |
| detection of hepatitis e virus in wild boar (sus scrofa) livers. | the routes of transmission of hepatitis e virus (hev) in industrialised countries are largely unknown, but several studies suggest that hev can be a porcine zoonosis. the aim of the present study was to determine the prevalence of hev in the wild boar (sus scrofa) and to determine the genetic relationships between hev sequences recovered from wild boars and from domestic pigs and humans. hev rna was detected by real time reverse transcriptase pcr in 7/285 (2.5%) liver samples from wild boars hun ... | 2010 | 19747861 |
| the effect of variable release kinetics on paclitaxel efficacy from a drug eluting stent in a porcine model. | aims: paclitaxel is a potent and effective inhibitor of neointimal proliferation after coronary stenting. the conor stent loaded with paclitaxel can be programmed with multi-parameter matrix of dose, temporal release profiles and release pathways. the aim of this study was to determine the most efficacious dose and release pattern of paclitaxel in a porcine model and parallels the pisces trial.methods: 32 farm pigs were implanted with conor stents loaded with 10 or 30 microg of paclitaxel with, ... | 2005 | 19758908 |
| mortality rates of interventional and surgical procedures performed in domestic juvenile farm pigs and yucatan mini-pigs. | perioperative and postoperative care are critical factors in cardiac catheterization and cardiothoracic surgical procedures. a retrospective analysis of mortality data in cardiovascular catheter and surgical studies performed in domestic juvenile swine (djs) and yucatan mini-swine (yms) was conducted. a total of 529 animals in 35 studies were included in the analysis, which included six study categories: coronary stenting (stent) and percutaneous transluminal coronary angioplasty (ptca) alone; s ... | 2003 | 19760832 |
| transmissibility studies of vacuolar changes in the rostral colliculus of pigs. | histopathological examinations of brains from healthy pigs have revealed localised vacuolar changes, predominantly in the rostral colliculus, that are similar to the neuropil vacuolation featured in the transmissible spongiform encephalopathies and have been described in pigs challenged parenterally with the agent causing bovine spongiform encephalopathy (bse). feedstuff containing bse-contaminated meat and bone meal (mbm) may have been fed to pigs prior to the ban of mammalian mbm in feed of fa ... | 2009 | 19765298 |
| kisspeptin signaling in the brain. | kisspeptin (a product of the kiss1 gene) and its receptor (gpr54 or kiss1r) have emerged as key players in the regulation of reproduction. mutations in humans or genetically targeted deletions in mice of either kiss1 or kiss1r cause profound hypogonadotropic hypogonadism. neurons that express kiss1/kisspeptin are found in discrete nuclei in the hypothalamus, as well as other brain regions in many vertebrates, and their distribution, regulation, and function varies widely across species. kisspept ... | 2009 | 19770291 |
| measurement of ninhydrin reactive nitrogen influx into gravesoil during aboveground and belowground carcass (sus domesticus) decomposition. | carcass decomposition results in the release of nitrogenous compounds into associated soil. the current study investigated the release of ninhydrin reactive nitrogen (nrn) following burial (approximately 40 cm depth) and decomposition on the soil surface. also investigated was the lateral extent of nrn in gravesoil. nrn concentration increased significantly in gravesoil collected from the center of graves (approximately 20 cm depth) during the initial two months of burial. a similar finding was ... | 2009 | 19773138 |
| vocal and locomotor responses of piglets to social isolation and reunion. | potentiation of infant isolation calls following a brief reunion with the mother is considered an index of filial bonding in altricial rodents. we investigated potentiation of isolation and reunion responses in 15-day-old unweaned domestic piglets (sus scrofa domesticus). when piglets were re-isolated following a brief, comforting reunion with their mother and littermates in their home pen, they displayed a persistence (relative potentiation) of calling and jumping. in contrast, when re-isolated ... | 2010 | 19774554 |
| enteric calicivirus and rotavirus infections in domestic pigs. | we report the prevalence of rotavirus and calicivirus infections, along with their respective association with diarrhoea in the porcine population of the region of northern spain. a total of 221 samples were collected at random from different farms in the region and from the main slaughterhouse facility in the city of zaragoza. faecal samples were scored as diarrhoeic or normal and grouped into five groups to match general farm management and age criteria: group i (suckling 0-4 weeks), group ii ... | 2010 | 19781118 |
| influence of portosystemic shunt on liver regeneration after hepatic resection in pigs. | the minimal amount of liver mass necessary for regeneration is still a matter of debate. the aim of the study was to analyze liver regeneration factors after extended resection with or without portosystemic shunt. | 2009 | 19794827 |
| detection of hepatitis e virus of genotype 3 in a farm pig in kinshasa (democratic republic of the congo). | autochthonous hepatitis e is an emerging disease in industrialized countries where a growing body of data indicates that pigs represent a reservoir for hepatitis e virus (hev) of genotype 3 or 4. in africa, only hev genotypes 1 and 2 have been identified in hepatitis e outbreaks as well as in sporadic cases. we aimed to investigate whether commercial pigs in sub-saharan africa might represent an hev reservoir using molecular assays. faecal samples from 40 pigs of the pietrain race housed in a fa ... | 2010 | 19800029 |
| long-term cognitive enrichment affects opioid receptor expression in the amygdala of domestic pigs. | enriching the housing environment by stimuli that challenge both reward and cognitive mechanisms may enhance behavioural experiences and can improve animal welfare, particularly in farm animals. a newly developed experimental feeding system for domestic pigs using food-rewarded learning of discriminatory and instrumental tasks enabled the animals to successfully master a cognitive challenge and to be rewarded ca. 30 times per day with small food portions. reward-related behaviour is expected to ... | 2010 | 19804558 |
| serial noninvasive in vivo positron emission tomographic tracking of percutaneously intramyocardially injected autologous porcine mesenchymal stem cells modified for transgene reporter gene expression. | porcine bone marrow-derived mesenchymal stem cells (mscs) were stably transfected with a lentiviral vector for transgene expression of the trifusion protein renilla luciferase, red fluorescent protein and herpes simplex truncated thymidine kinase (lv-rl-rfp-ttk; positron emission tomography [pet] reporter gene) for in vivo noninvasive tracking of the intramyocardially delivered msc fate. | 2008 | 19808526 |