| the p protein of spring viremia of carp virus negatively regulates the fish interferon response by inhibiting the kinase activity of tank-binding kinase 1. | spring viremia of carp virus (svcv) is an efficient pathogen causing high mortality in the common carp. fish interferon (ifn) is a powerful cytokine enabling host cells to establish an antiviral response; therefore, the strategies that svcv uses to avoid the cellular ifn response were investigated. here, we report that the svcv p protein is phosphorylated by cellular tank-binding kinase 1 (tbk1), which decreases ifn regulatory factor 3 (irf3) phosphorylation and suppresses ifn production. first, ... | 2016 | 27654289 |
| detection of spring viraemia of carp virus in imported amphibians reveals an unanticipated foreign animal disease threat. | global translocation of plants and animals is a well-recognized mechanism for introduction of pathogens into new regions. to mitigate this risk, various tools such as preshipment health certificates, quarantines, screening for specific disease agents and outright bans have been implemented. however, such measures only target known infectious agents and their hosts and may fail to prevent translocation of even well-recognized pathogens if they are carried by novel host species. in a recent exampl ... | 2016 | 27599472 |
| zebrafish irf1, irf3, and irf7 differentially regulate ifnφ1 and ifnφ3 expression through assembly of homo- or heteroprotein complexes. | in mammals, ifn regulatory factor (irf)1, irf3, and irf7 are three critical transcription factors that are pivotal for cooperative regulation of the type i ifn response. in this study, we explored the relative contribution of zebrafish (danio rerio) irf1 (drirf1), irf3 (drirf3), and irf7 (drirf7) (drirf1/3/7) to zebrafish ifnφ1 (drifnφ1) and ifnφ3 (drifnφ3) (drifnφ1/3) activation. following spring viremia of carp virus infection, drifnφ1/3 and drirf1/3/7 transcripts are significantly induced in ... | 2016 | 27496972 |
| emergence of a new rhabdovirus associated with mass mortalities in eelpout (zoarces viviparous) in the baltic sea. | we report the first description of a new rhabdoviridae tentatively named eelpout rhabdovirus (eprv genus perhabdovirus). this virus was associated with mass mortalities in eelpout (zoarces viviparous, linnaeus) along the swedish baltic sea coast line in 2014. diseased fish showed signs of central nervous system infection, and brain lesions were confirmed by histology. a cytopathogenic effect was observed in cell culture, but elisas for the epizootic piscine viral haemorrhagic septicaemia virus ( ... | 2017 | 27416895 |
| toll-like receptors and interferon associated immune factors responses to spring viraemia of carp virus infection in common carp (cyprinus carpio). | pattern recognition receptor (prr) toll-like receptors (tlrs), antiviral agent interferon (ifn) and the effector ifn stimulated genes (isgs) play a fundamental role in the innate immune response against viruses among all vertebrate classes. common carp is a host for spring viraemia of carp virus (rhabdovirus carpio, svcv), which belong to rhabdoviridae family. the present in-vivo experiment was conducted to investigate the expression of these innate immune factors in early phase as well as durin ... | 2016 | 27263115 |
| restrictions on the importation of zebrafish into canada associated with spring viremia of carp virus. | the zebrafish model system is helping researchers improve the health and welfare of people and animals and has become indispensable for advancing biomedical research. as genetic engineering is both resource intensive and time-consuming, sharing successfully developed genetically modified zebrafish lines throughout the international community is critical to research efficiency and to maximizing the millions of dollars in research funding. new restrictions on importation of zebrafish into canada b ... | 2016 | 27248438 |
| spring viremia of carp virus n protein suppresses fish ifnφ1 production by targeting the mitochondrial antiviral signaling protein. | for a virus to replicate efficiently, it must try and inhibit host ifn expression because ifn is an important host defense at early stages after viral infection. for aquatic viruses, the mechanisms used to escape the hosts ifn system are still unclear. in this study, we show that the n protein of spring viremia of carp virus (svcv) inhibits zebrafish ifnφ1 production by degrading the mitochondrial antiviral signaling protein (mavs). first, the upregulation of ifnφ1 promoter activity stimulated b ... | 2016 | 26994222 |
| spring viraemia of carp virus: recent advances. | spring viraemia of carp is an environmentally and economically important disease affecting cyprinids, primarily common carp (cyprinus carpio). the causative agent of this disease is spring viraemia of carp virus (svcv) - a member of the genus vesiculovirus of the family rhabdoviridae. the disease is presently endemic in europe, america and several asian countries, where it causes significant morbidity and mortality in affected fish. svcv infection is generally associated with exophthalmia; abdom ... | 2016 | 26905065 |
| genetic diversity of spring viraemia of carp virus isolates based on the glycoprotein gene. | | 2016 | 26791607 |
| an n-targeting real-time pcr strategy for the accurate detection of spring viremia of carp virus. | spring viremia of carp virus (svcv) is a highly pathogenic agent of several economically important cyprinidae fish species. currently, there are no effective vaccines or drugs for this virus, and prevention of the disease mostly relies on prompt diagnosis. previously, nested rt-pcr and rt-qpcr detection methods based on the glycoprotein gene g have been developed. however, the high genetic diversity of the g gene seriously limits the reliability of those methods. compared with the g gene, phylog ... | 2016 | 26717888 |
| pathogenesis of spring viremia of carp virus in emerald shiner notropis atherinoides rafinesque, fathead minnow pimephales promelas rafinesque and white sucker catostomus commersonii (lacepede). | spring viremia of carp (svc) is a reportable disease to the world organization of animal health (oie) as it is known to cause significant international economic impact. in canada, the first and only isolation of svc virus (svcv) was in 2006, from common carp cyprinus carpio l., at hamilton harbour, lake ontario. the susceptibility of fathead minnow pimephales promelas rafinesque, emerald shiner notropis atherinoides rafinesque and white sucker catostomus commersonii (lacepede) to intraperitoneal ... | 2016 | 26411333 |
| development of an improved rt-pcr for specific detection of spring viraemia of carp virus. | spring viraemia of carp (svc) is a rhabdovirus infection, which has a significant economic impact in pond cultures of carp in europe and western independent states of the former soviet union. the causative agent of svc, spring viraemia of carp virus (svcv), has been divided into four subgroups, ia, ib, ic and id, on the basis of glycoprotein (g) protein gene sequences. in this study, a new primer set was designed from a g gene sequence of svcv to identify the four subtypes of svcv by reverse tra ... | 2016 | 25832786 |
| establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish (misgurnus anguillicaudatus). | continuous fin cell lines from diploid, triploid, and tetraploid oriental weatherfish, misgurnus anguillicaudatus, were established and characterized. the cell lines, designated dimf, trmf, and temf, respectively, were subcultured more than 80 times since initiation in october 2012 and were preserved at the china center for type culture collection as sample numbers c2013109, c2013110, c2013111, respectively. the cell lines consist predominantly of fibroblast-like cells. at the 50th passage, the ... | 2015 | 25721100 |
| characterization of two thymosins as immune-related genes in common carp (cyprinus carpio l.). | prothymosin alpha (protα) and thymosin beta (tβ) belong to thymosin family, which consists of a series of highly conserved peptides involved in stimulating immune responses. protα b and tβ are still poorly studied in teleost. here, the full-length cdnas of protα b and tβ-like (tβ-l) were cloned and identified in common carp (cyprinus carpio l.). the expressions of carp protα b and tβ-l exhibited rise-fall pattern and then trended to be stable during early development. after spring viraemia of ca ... | 2015 | 25596145 |
| zebrafish irf1 regulates ifn antiviral response through binding to ifnϕ1 and ifnϕ3 promoters downstream of myd88 signaling. | in mammals, type i ifns (mainly ifn-α/β) are primarily regulated by transcription factors of the ifn regulatory factor (irf) family. fish ifns do not show a one-to-one orthologous relationship with mammalian type i ifn homologues. using a bacterial one-hybrid reporter screening system and an overexpression approach to explore the molecular mechanism underlying fish ifn induction, we identified zebrafish danio rerio irf (drirf)1 as a positive regulator of the fish ifn antiviral response. among 12 ... | 2015 | 25535281 |
| spring viraemia of carp virus induces autophagy for necessary viral replication. | outbreaks of spring viraemia of carp virus (svcv) in several carp species and other cultivated fish can cause significant mortality and jeopardize the billion-dollar worldwide fish industry. spring viraemia of carp virus, also known as rhabdovirus carpio, is a bullet-shaped rna virus that enters and amplifies in gill epithelium and later spreads to internal organs. young fish under stressed conditions (spring cold water, etc.) are more vulnerable to svcv-induced lethality because of their lack o ... | 2015 | 25376386 |
| transcriptome analysis of epithelioma papulosum cyprini cells after svcv infection. | spring viraemia of carp virus (svcv) has been identified as the causative agent of spring viraemia of carp (svc) and it has caused significant losses in the cultured common carp (cyprinus carpio) industry. the molecular mechanisms that underlie the pathogenesis of the disease remain poorly understood. in this study, deep rna sequencing was used to analyse the transcriptome and gene expression profile of epc cells at progressive times after svcv infection. this study addressed the complexity of v ... | 2014 | 25344771 |
| microrna profile analysis of epithelioma papulosum cyprini cell line before and after svcv infection. | micrornas (mirnas) play significant roles in regulating almost all of the biological processes in eukaryotes. an accumulating body of evidence shows that mirnas are associated with cellular changes following viral infection. spring viremia of carp virus (svcv) is the pathogen of spring viremia of carp (svc), which results in heavy losses in the cultured common carp (cyprinus carpio) industry in many countries. to study the involvement of mirnas during svcv infection, we adopted the solexa sequen ... | 2015 | 25291211 |
| vhsv g glycoprotein major determinants implicated in triggering the host type i ifn antiviral response as dna vaccine molecular adjuvants. | we have recently identified the two major determinants of the glycoprotein g of the viral hemorrhagic septicaemia rhabdovirus (gpgvhsv), peptides p31 and p33 implicated in triggering the host type i ifn antiviral response associated to these rhabdoviral antigens. with the aim to investigate the properties of these viral glycoprotein regions as dna molecular adjuvants, their corresponding cdna sequences were cloned into a plasmid (pmcv1.4) flanked by the signal peptide and transmembrane sequences ... | 2014 | 25203447 |
| up-regulation of nuclear factor e2-related factor 2 upon svcv infection. | nuclear factor e2 - related factor 2 (nrf2) is a crucial transcription factor that regulates the basal and inducible expression of many antioxidant response element (are)-dependent genes, including heme oxygenase-1 (ho-1) and superoxide dismutase 1 (sod1). the nrf2/are pathway has been regarded as a critical switch in the initiation of cellular defence systems for surviving oxidative insults and viral infection. in this study, the nrf2 gene of epc cells, which is originally derived from pimephal ... | 2014 | 25038284 |
| ifn regulatory factor 10 is a negative regulator of the ifn responses in fish. | ifn regulatory factor (irf) 10 belongs to the irf family and exists exclusively in birds and fish. most irfs have been identified as critical regulators in the ifn responses in both fish and mammals; however, the role of irf10 is unclear. in this study, we identified irf10 in zebrafish (danio rerio) and found that it serves as a negative regulator to balance the innate antiviral immune responses. zebrafish irf10 (drirf10) was induced by intracellular polyinosinic:polycytidylic acid in zf4 (zebra ... | 2014 | 24958903 |
| genomic evidence of homologous recombination in spring viremia of carp virus: a negatively single stranded rna virus. | a new strain of spring viraemia of carp virus, denominated svcv-265, was isolated from an ornamental common carp (cyprinus carpio) in shanghai, china, 2013. the isolate could produce obvious cytopathic effects on epc cells, while was shown to be of low virulence for juvenile koi. complete genome sequencing revealed the genome of the svcv-265 strain is 11,029 nucleotides in length and phylogenetic analysis showed the isolate was clustered within asia clade but was divergent from chinese a1, a2 an ... | 2014 | 24954789 |
| selection and characterization of single-chain recombinant antibodies against infectious haematopoietic necrosis virus from mouse phage display library. | six single-chain fragment variable (scfv) antibodies against infectious haematopoietic necrosis virus (ihnv) were selected from an antibody phage display library by phage display technology. the soluble scfv antibodies showed a molecular weight 32kda by western blot. dot blot analysis revealed that the six scfv antibodies could recognize ihnv. for enzyme linked immunosorbent assay (elisa), four scfv antibodies (p1a4, p1a12, p1d5 and p3e2) showed cross-reactivity with spring viraemia of carp viru ... | 2014 | 24747010 |
| caspian white fish (rutilus frisii kutum) as a host for spring viraemia of carp virus. | rutilus frisii kutum is a fish of the cyprinidae family which is native in caspian sea and commercially cultured in iran. this study was conducted to investigate susceptibility of caspian white fish to spring viraemia of carp virus (svcv) infection and to evaluate influence of different challenge routes on virulence of the virus. fingerlings were infected by immersion, intra-peritoneal (i.p.) injection, cohabitation and orally. dead and surviving fish were collected for histological examination ... | 2014 | 24685241 |
| codon usage bias of the phosphoprotein gene of spring viraemia of carp virus and high codon adaptation to the host. | in this study, we calculated the relative synonymous codon usage (rscu) value and the effective number of codons (enc) value to carry out principal component analysis (pca) and correlation analysis of the codon usage pattern of the phosphoprotein gene (p gene) of spring viraemia of carp virus (svcv). the synonymous codon usage pattern in p genes is geography-specific, based on pca analysis. the high correlation between (g + c)1,2 % and (g + c)3 % suggests that mutational pressure rather than nat ... | 2014 | 24519460 |
| experimental susceptibility of caspian white fish, rutilus frisii kutum to spring viraemia of carp virus. | caspian white fish (rutilus frisii kutum) is a fish of the family cyprinidae, which is commercially harvested from the caspian sea. experimental infection with spring viraemia of carp virus (svcv) was conducted in order to examine susceptibility of caspian white fish and clinical impacts of infection. fingerling fish were injected intra-peritoneally or waterborne-exposed with svcv and were monitored daily for 7 weeks. dead fish and those survived at the end of experimental period were collected ... | 2014 | 24426310 |
| selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library. | antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (svcv) by phage display technology. eight positive clones which could produce soluble single-chain fragment variable (scfv) antibody induced by isopropyl-beta-d-thiogalactopyranoside (iptg) were obtained. dot blot results showed that the eight scfv antibodies could recognize svcv. the soluble scfv antibodies showed a molecular weight 29 kd by western blot. all scfv antibodies could ... | 2013 | 23994147 |
| proteomic analysis of epithelioma papulosum cyprini cells infected with spring viremia of carp virus. | spring viremia of carp (svc), caused by spring viremia of carp virus (svcv) is an important disease due to its drastic effects on carp fisheries in many countries. to better understand molecular responses to svcv infection, two dimensional electrophoresis (2-de) and maldi-tof/tof were performed to investigate altered proteins in epithelioma papulosum cyprini cells (epcs). differentially expressed proteins in mock-infected epcs and svcv-infected epcs were compared. a total of 54 differentially ex ... | 2013 | 23583725 |
| spring viremia of carp virus in minnehaha creek, minnesota. | spring viremia of carp virus (svcv) causes a highly contagious and serious disease of freshwater cyprinid fishes, generating significant economic and ecological impacts throughout the world. the svcv is therefore listed as a notifiable pathogen by the international organization for animal health. in june 2011, a significant mortality event of wild common carp cyprinus carpio occurred in minnehaha creek near its confluence with mississippi river pool 2 in minneapolis, minnesota. clinical signs of ... | 2012 | 23072656 |
| direct detection of unamplified spring viraemia of carp virus rna using unmodified gold nanoparticles. | spring viraemia of carp (svc) is a viral disease that mainly affects carp cyprinus carpio and other cyprinid fish, causing severe economic losses. rapid detection and identification of spring viraemia of carp virus (svcv) is crucial for effective disease management. recent advances in nanoscience are having a significant impact on many scientific fields, especially biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecular detection. single- and double-stra ... | 2012 | 22885508 |
| fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in staphylococcus aureus media. | fish peptone was produced using enzymatic hydrolysis of silver carp filleting by-products by alcalase and trypsin. also, the efficiency of the hydrolysates as a nitrogen source in staphylococcus aureus medium was compared with commercial tsb. the results indicated that the protein hydrolysate from alcalase and trypsin had high protein content (92.92%, 91.53 respectively), and degree of hydrolysis (4.94%, 4.6% respectively).the results showed that silver carp filleting waste can be an efficient s ... | 2015 | 25838893 |
| structural changes in freshwater fish and chironomids exposed to bacterial exotoxins. | mass fish mortalities have been reported in the past decade from british waters, often coinciding with blooms of filamentous actinobacteria, particularly strains of streptomyces griseus. the present study has shown that some fractions of the exudate of s. griseus, prepared after a series of high pressure liquid chromatography (hplc) separations, and analysed with liquid chromatography mass spectrometry (lc-ms), induced pathological changes to the gills of carp and/or tench fry following exposure ... | 2012 | 22381615 |
| detection of toxoplasma gondii in shellfish and fish in parts of china. | in this study, a total of 3432 aquatic animals covering eight species were tested by specific polymerase chain reaction (pcr) assays to investigate the presence of toxoplasma gondii, in china. the results showed that, out of 618 procambarus clarkii samples collected from the different areas in southeast china, four samples from jiangxi province were positive. of 456 hypophthalmichthys molitrix samples, one sample from jiangsu province was positive. in addition, there was only one positive sample ... | 2014 | 24331298 |
| transcriptome sequence of the bloodstream form of trypanoplasma borreli, a hematozoic parasite of fish transmitted by leeches. | here, we report a transcriptome sequence of trypanoplasma borreli isolated from its natural host, the common carp, cyprinus carpio the transcriptome allows an analysis of abundant cell surface proteins and acts as a comparator for understanding the evolution and pathogenicity of other kinetoplastida, including several that infect humans. | 2017 | 28254980 |
| immune-relevant thrombocytes of common carp undergo parasite-induced nitric oxide-mediated apoptosis. | common carp thrombocytes account for 30-40% of peripheral blood leukocytes and are abundant in the healthy animals' spleen, the thrombopoietic organ. we show that, ex vivo, thrombocytes from healthy carp express a large number of immune-relevant genes, among which several cytokines and toll-like receptors, clearly pointing at immune functions of carp thrombocytes. few studies have described the role of fish thrombocytes during infection. carp are natural host to two different but related protozo ... | 2015 | 25681740 |
| identification and functional characterization of nonmammalian toll-like receptor 20. | like other vertebrate toll-like receptors (tlrs), the tlrs of teleost fish can be subdivided into six major families, each of which recognize a general class of molecular patterns. however, there also are a number of tlrs with unknown function, the presence of which seems unique to the bony fish, among which is tlr20. we identified full-length complementary dna (cdna) sequences for tlr20 of zebrafish and common carp, two closely related fish species. zebrafish have six copies of tlr20, whereas c ... | 2014 | 24327191 |
| immunity against selected piscine flagellates. | this discussion is on immune response to amyloodinium ocellatum, cryptobia salmositica, trypanoplasma borreli and trypanosoma carassii. piscidin and histone-like proteins enhance innate resistance to amyloodinium. fish that are naturally resistant to cryptobia and trypanoplasma can be bred. cryptobia resistance in charr is controlled by a dominant mendelian locus and protection is via the alternative pathway of complement activation. studies on cryptobia-tolerant charr may lead to production of ... | 2014 | 23872230 |
| goldfish (carassius auratus l.) possess natural antibodies with trypanocidal activity towards trypanosoma carassii in vitro. | natural infection of cyprinids, such as carp, with the extracellular protozoan parasite trypanosoma carassii can attain up to 100% prevalence and cause significant host morbidity and mortality, particularly in aquaculture settings. host recovery from t. carassii infection has been shown to be antibody (immunoglobulin m; igm)-mediated, conferring long-term immunity in recovered animals upon challenge. to assess the role of igm in parasite clearance in the goldfish, igm was purified by peg-6000 pr ... | 2013 | 23333358 |
| isolation and characterization of glycosylphosphatidylinositol-anchored, mucin-like surface glycoproteins from bloodstream forms of the freshwater-fish parasite trypanosoma carassii. | wild and farmed freshwater fishes are widely and heavily parasitized by the haemoflagellate trypanosoma carassii. in contrast, common carp, a natural host, can effectively control experimental infections by the production of specific anti-parasite antibodies. in this study we have identified and partially characterized mucin-like glycoproteins which are expressed in high abundance [(6. 0+/-1.7)x10(6) molecules.cell(-1)] at the surface of the bloodstream trypomastigote stage of the parasite. the ... | 2000 | 10642530 |
| cultivation of bloodstream forms of trypanosoma carassii, a common parasite of freshwater fish. | trypanosoma carassii (syn. t. danilewskyi) is a widespread parasite of carp and other cyprinid as well as some noncyprinid freshwater fish. it lives extracellularly in the blood and tissues of its hosts, causing chronic infections. in this paper the isolation of t. carassii from fish blood and the propagation and cloning of bloodstream forms in vitro are described. by several criteria, cultured and fish-derived trypomastigotes are indistinguishable. the culture system should be useful for the bi ... | 1998 | 9610629 |
| autophagy-inducing peptides from mammalian vsv and fish vhsv rhabdoviral g glycoproteins (g) as models for the development of new therapeutic molecules. | it has not been elucidated whether or not autophagy is induced by rhabdoviral g glycoproteins (g) in vertebrate organisms for which rhabdovirus infection is lethal. our work provides the first evidence that both mammalian (vesicular stomatitis virus, vsv) and fish (viral hemorrhagic septicemia virus, vhsv, and spring viremia carp virus, svcv) rhabdoviral gs induce an autophagic antiviral program in vertebrate cell lines. the transcriptomic profiles obtained from zebrafish genetically immunized w ... | 2014 | 25046110 |
| characterization and expression pattern of a novel β-defensin in common carp (cyprinus carpio l.): implications for its role in mucosal immunity. | β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems of vertebrates. here, we report the cloning and identification of a β-defensin 3 cdna sequence from the common carp (cyprinus carpio l.). sequence alignment and phylogenetic analysis indicated that this β-defensin 3 belonged to the bd-2 group of fish. real-time pcr showed that the β-defensin 3 mrna was expressed in all the tissues of normal common carp that we ex ... | 2014 | 25036829 |
| molecular characterization of leap-2 cdna in common carp (cyprinus carpio l.) and the differential expression upon a vibrio anguillarum stimulus; indications for a significant immune role in skin. | leap-2 is a cysteine-rich cationic antimicrobial peptide (amp) playing an important role in host innate immune system. leap-2 genes have been identified from higher vertebrates and several fish species. here we report the cloning and identification of two leap-2 cdna sequences from the liver of common carp (cyprinus carpio l.). the leap-2a cdna was 1325 bp long and contained an orf of 279 bp encoding a protein of 92 amino acids. the leap-2b cdna was 608 bp long and contained an orf of 276 bp enc ... | 2014 | 24418455 |
| molecular characterization and expression analysis of toll-like receptor 21 cdna from paralichthys olivaceus. | toll-like receptor (tlr) is believed to play crucial role in host defense of pathogenic microbes in innate immune system. in the present study, the full-length cdna of paralichthys olivaceus toll-like receptor 21 (po-tlr21) was cloned by homology cloning and rapid amplification of cdna ends (race) technique. the po-tlr21 cdna sequence was 3687 bp, containing an open reading frame of 2922 bp encoding 973 amino acids. tmhmm and smart program analysis indicated that protein contained one transmembr ... | 2013 | 23880453 |
| molecular characterization of hepcidin gene in common carp (cyprinus carpio l.) and its expression pattern responding to bacterial challenge. | hepcidin is a cysteine-rich cationic antimicrobial peptide (amp), which plays an important role in host innate immune system and iron regulation. a great many of hepcidin genes have been identified from vertebrates, including various fish species. here we report the cloning and identification of a hepcidin cdna from the liver of common carp (cyprinus carpio l.). the full-length cdna of the common carp hepcidin was 647 bp, which contained an orf of 276 bp encoding a prepropeptide of 91 amino acid ... | 2013 | 23851290 |
| virulence of viral hemorrhagic septicemia virus (vhsv) genotypes ia, iva, ivb, and ivc in five fish species. | the susceptibility of yellow perch perca flavescens, rainbow trout oncorhynchus mykiss, chinook salmon o. tshawytscha, koi cyprinus carpio koi, and pacific herring clupea pallasii to 4 strains of viral hemorrhagic septicemia virus (vhsv) was assessed. fish were challenged via intraperitoneal injection with high (1 × 106 plaque-forming units, pfu) and low (1 × 103 pfu) doses of a european strain (genotype ia), and north american strains from the west coast (genotype iva), great lakes (genotype iv ... | 2013 | 24334352 |
| experimental infection of koi carp with viral hemorrhagic septicemia virus type ivb. | viral hemorrhagic septicemia virus (vhsv) type ivb has a wide host range that includes at least three cyprinid species: fathead minnow pimephales promelas, emerald shiner notropis atherinoides, and bluntnose minnow p. notatus. to date, vhsv ivb has only been found in wild fish. however, the possibility of infection in culture facilities remains. koi carp cyprinus carpio are a major ornamental aquaculture species in the united states; however, their potential to become infected with vhsv ivb has ... | 2013 | 23289973 |
| improving the safety of viral dna vaccines: development of vectors containing both 5' and 3' homologous regulatory sequences from non-viral origin. | although some dna vaccines have proved to be very efficient in field trials, their authorisation still remains limited to a few countries. this is in part due to safety issues because most of them contain viral regulatory sequences to driving the expression of the encoded antigen. this is the case of the only dna vaccine against a fish rhabdovirus (a negative ssrna virus), authorised in canada, despite the important economic losses that these viruses cause to aquaculture all over the world. in a ... | 2013 | 23001057 |
| persistence of viral rna in fish infected with vhsv-ivb at 15°c and then moved to warmer temperatures after the onset of disease. | smallmouth bass, micropterus dolomieu lacepède, bluegill, lepomis macrochirus rafinesque (coppernose strain), koi carp, cyprinus carpio l., and channel catfish ictalurus punctatus (rafinesque), were infected by intraperitoneal injection with viral haemorrhagic septicaemia virus genotype ivb (vhsv-ivb) at 15 °c. when clinical signs of disease developed, one-third of the fish was moved to 20°c and one-third to 25°c. mortality in challenged fish at all three temperatures ranged from 25 to 45% in sm ... | 2012 | 22582793 |
| in vitro cytotoxicity and multiplex pcr detection of virulence factors of yersinia ruckeri isolated from rainbow trout in north west germany. | the aim of this study was to investigate differences in presence and expression of virulence factors between biotype 1 and 2 strains of 82 yersinia (y.) ruckeri isolates, collected from north west germany during the period of 2004-2012, and to analyze the cytotoxicity of these strains to different fish cell lines. the common virulence factor genes, such as yhla and yhlb encoding for hemolysin yhla, rucc and rupg encoding for ruckerbactin, yrp1 and yrpdef for abc exporter protein system, and two ... | 2014 | 24881275 |
| khawia abbottinae sp. n. (cestoda: caryophyllidea) from the chinese false gudgeon abbottina rivularis (cyprinidae: gobioninae) in china: morphological and molecular data. | khawia abbottinae sp. n. is described from the chinese false gudgeon, abbottina rivularis (basilewsky) (cyprinidae: gobioninae), from the yangtze river basin in china. the new species can be distinguished from the congeneric species mainly by the arrangements of the testes, which form two longitudinal bands (other congeneric species have the testes irregularly scattered throughout the testicular region) and their number (at maximum 85 testes versus at least 160 in the other khawia spp.), and the ... | 2013 | 23724733 |
| effects of portable solar water quality control machines on aquaculture ponds. | the effects of a portable solar water quality control machine (pswm) on water quality and sediment of aquaculture ponds were studied in bream aquaculture ponds in shanghai, china. pswm operation reduced the temperature and dissolved oxygen (do) differences between upper and lower water levels. concentrations of nh4(+)-n, no2(-)-n, tn, tp, cod and tss increased rapidly and reached maximums at 12 h. the density and biomass of phytoplankton and levels of chlorophyll a reached maximums after 40 h of ... | 2017 | 27928749 |
| who is who: an anomalous predator-prey role exchange between cyprinids and perch. | piscivory in cyprinids (cyprinidae) is extremely rare. specifically, common bream (abramis brama) and common carp (cyprinus carpio) are zooplanktivorous fish in deep lentic waters. nevertheless, we observed predation by these two cyprinids under natural conditions in the vír reservoir, czech republic. we conducted diet analysis for cyprinids caught by trawling and gillnets and the large amount of young-of-the-year (yoy) perch (perca fluviatilis), with sizes of 37-52 mm standard length, were foun ... | 2016 | 27276078 |
| design and application of a solar mobile pond aquaculture water quality-regulation machine based in bream pond aquaculture. | bream pond aquaculture plays a very important role in china's aquaculture industry and is the main source of aquatic products. to regulate and control pond water quality and sediment, a movable solar pond aquaculture water quality regulation machine (smwm) was designed and used. this machine is solar-powered and moves on water, and its primary components are a solar power supply device, a sediment lifting device, a mechanism for walking on the water's surface and a control system. the solar powe ... | 2016 | 26789004 |
| role of peptidases of the intestinal microflora and prey in temperature adaptations of the digestive system in planktivorous and benthivorous fish. | many fish enzymatic systems possess limited adaptations to low temperature; however, little data are available to judge whether enzymes of fish prey and intestinal microbiota can mitigate this deficiency. in this study, the activity of serine peptidases (casein-lytic, mainly trypsin and hemoglobin-lytic, mainly chymotrypsin) of intestinal mucosa, chyme and intestinal microflora in four species of planktivorous (blue bream) and benthivorous (roach, crucian carp, perch) was investigated across a w ... | 2015 | 26251288 |
| persistent organic pollutants in muscle of fish collected from the nové mlýny reservoir in southern moravia, czech republic. | the aim of the present study was to investigate the content of organochlorine pesticides (ocps) and polychlorinated biphenyls (pcbs) in muscle tissues of fish, i.e. carp (cyprinus carpio, l.), bream (abramis brama, l.) and pike perch (stizostedion lucioperca, l.) from the middle nové mlýny reservoir and compare our results with previous corresponding studies. samples were analysed by gas chromatography with ion trap tandem mass spectrometry. the highest contents of all pollutants were determined ... | 2015 | 26092236 |
| biomass and abundance biases in european standard gillnet sampling. | the european standard en 14757 recommends gillnet mesh sizes that range from 5 to 55mm (knot-to-knot) for the standard monitoring of fish assemblages and suggests adding gillnets with larger mesh sizes if necessary. our research showed that the recommended range of mesh sizes did not provide a representative picture of fish sizes for larger species that commonly occur in continental europe. we developed a novel, large mesh gillnet which consists of mesh sizes 70, 90, 110 and 135mm (knot to knot, ... | 2015 | 25793776 |
| [differences in the infestation rate of young cyprinid fishes (cypriniformes) by metacercaria of posthodiplostomum cuticola (digenea, diplostomatidae) in river and lake systems of the lake chany basin (western siberia)]. | a total of 12 fish species were studied for the invasion of p. cuticola (nordmann, 1832) metacercariae in the chany lake estuaries, river and lake systems during different periods of water level. all infected individuals were represented by juveniles of the family cyprinidae, except for adults of the dace leuciscus leuciscus. (l.). under an average water level (2010) the highest rate of fish invasion was revealed in the estuary zone, where the prevalence of infection (pi) constituted 37.5%, 13.4 ... | 2015 | 25693328 |
| [adaptive increase of serotonergic system activity in tissues of half-migratory and migratory fish at increased water salinity]. | the article deals with studies of the serotoninergic system activity in different tissues of half-migratory fish--the caspian roach (rutilus rutilus caspicus) and carpbream (abramis brama orientalis)--and migratory fish--shemaya (chalcalburnus chalcoides) caught in fresh and brackish waters, as well as in the common carp (cyprinus carpio l.) tissues under effect of brackish water in model experiments. using indirect solid-phase elisa-test, the serotoninergic system activity was evaluated by meas ... | 2014 | 25509051 |
| [adaptive increase of serotonergic system activity in tissues of half-migratory and migratory fish at increased water salinity]. | the article deals with studies of the serotoninergic system activity in different tissues of half-migratory fish--the caspian roach (rutilus rutilus caspicus) and carpbream (abramis brama orientalis)--and migratory fish--shemaya (chalcalburnus chalcoides) caught in fresh and brackish waters, as well as in the common carp (cyprinus carpio l.) tissues under effect of brackish water in model experiments. using indirect solid-phase elisa-test, the serotoninergic system activity was evaluated by meas ... | 2014 | 25490850 |
| pcb pollutants: reduce consumption of some freshwater fish. | the accumulation of polychlorinated biphenyls (pcbs) in some freshwater fish exposes consumers to these organic pollutants, which are known to have a variety of harmful effects on health. a study conducted in anglers and consumers of freshwater fish in france confirmed that people who eat these fish can have blood pcb levels that exceed the critical concentration thresholds below which health risks are regarded as negligible. the french agency for food, environmental and occupational health and ... | 2014 | 25325129 |
| characterizing the transcriptome of yellow-cheek carp (elopichthys bambusa) enables evolutionary analyses within endemic east asian cyprinidae. | the identification of genes that may be responsible for the divergence of closely related species is one of the central goals of evolutionary biology. the species of endemic east asian cyprinidae diverged less than 8millionyears ago, and the morphological differences among these species are great. however, the genetic basis of their divergence remains unknown. in this report, we investigated the transcriptome of one endemic east asian cyprinid - the yellow-cheek carp elopichthys bambusa. a compa ... | 2014 | 24973763 |
| effect of temperature on hypoxia tolerance and its underlying biochemical mechanism in two juvenile cyprinids exhibiting distinct hypoxia sensitivities. | it is increasingly important to investigate the effect of temperature on hypoxia tolerance in fish species, as worldwide hypoxia worsens with increases in global warming. we selected the hypoxia-tolerant crucian carp (carassius carassius) and the hypoxia-sensitive chinese bream (parabramis pekinensis) as model fish and investigated their hypoxia tolerance based on the critical oxygen tension of the routine metabolic rate (m˙o2rout) (pcrit), aquatic surface respiration (asrcrit) and loss of equil ... | 2015 | 24853206 |
| molecular cloning, characterization and functional assessment of the myosin light polypeptide chain 2 (mylz2) promoter of farmed carp, labeo rohita. | we cloned the 5'-flanking region (1.2 kb) of a muscle-specific gene, encoding myosin light chain 2 polypeptide (mylz2) of a farmed carp, labeo rohita (rohu). sequence analysis using transfac-database search identified the consensus cis acting regulatory elements of tata-box and e (canntg)-box, including the monocyte enhancer factor 2 motif, implying that it is likely to be a functional promoter. the proximal promoter (~620 bp) was highly homologous with that of danio rerio (zebrafish) as compare ... | 2014 | 24740361 |
| interspecific differences in hypoxia-induced gill remodeling in carp. | the gills of many fish, but in particular those of crucian carp (carassius carassius) and goldfish (carassius auratus), are capable of extensive remodeling in response to changes in oxygen (o2), temperature, and exercise. in this study, we investigated the interspecific variation in hypoxia-induced gill modeling and hypoxia tolerance in 10 closely related groups of cyprinids (nine species, with two strains of cyprinus carpio). there was significant variation in hypoxia tolerance, measured as the ... | 2016 | 24241069 |
| content of polychlorinated dibenzo-p-dioxins, dibenzofurans and dioxin-like polychlorinated biphenyls in fish from latvian lakes. | seventeen polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (pcdd/pcdfs) of the highest priority as well as twelve dioxin-like polychlorinated biphenyls (dl-pcbs) were analyzed in the muscle tissues of the following freshwater fish species sampled from eleven latvian freshwater lakes: perch (perca flavescens), carp (cyprinus carpio), eel (anguilla rostrata), bream (abramis brama), chub (leuciscus cephalus), pike (esox lucius), sheatfish (silurus glanis) and roach (rutilus). to ... | 2013 | 23336927 |
| distribution and relationships of as, cd, pb and hg in freshwater fish from five french fishing areas. | the concentrations of arsenic, cadmium, mercury and lead in 149 muscle samples of eight freshwater fish species (european eel, bream, common carp, european catfish, roach, perch, pike and pikeperch) from five different french fishing areas from contaminated and control sites were measured by inductively coupled plasma mass spectrometry after microwave digestion under pressure. no significant correlation was found between the condition factor (cf), based on the length-mass relationship, and as, c ... | 2013 | 23177713 |
| trypsin inhibitory activity and gel-enhancing effect of sarcoplasmic proteins from common carp. | proteinase inhibitory activity of sarcoplasmic protein (sp) extracted from common carp (cyprinus carpio) muscle and its gel-improving ability were investigated. sps displayed 89% and 54% inhibitory activity toward trypsin at 40 and 65 °c, respectively. protein bands with molecular mass of 69, 50, 44, 41, and 35 kda appeared on trypsin inhibitory activity staining under nonreducing condition when incubated at 40 °c, while 2 protein bands at 54 and 35 kda were observed at 65 °c. addition of sp at ... | 2012 | 22978469 |
| the bioconcentration and bioaccumulation factors for molybdenum in the aquatic environment from natural environmental concentrations up to the toxicity boundary. | in a regulatory context, bioaccumulation or bioconcentration factors are used for considering secondary poisoning potential and assessing risks to human health via the food chain. in this paper, literature data on the bioaccumulation of molybdenum in the aquatic organisms are reviewed and assessed for relevance and reliability. the data available in the literature were generated at exposure concentrations below those recommended in the reach registration dossiers for molybdenum compounds i.e. pn ... | 2012 | 22846769 |
| metals in edible fish from vistula river and dead vistula river channel, baltic sea. | metals including al, ba, ca, cd, co, cr, cu, fe, hg, k, mg, mn, na, ni, pb, sr and zn were determined in muscle tissue of 12 fish species by inductively coupled plasma-atomic emission spectroscopy (icp-aes) and cold vapour-atomic absorption spectroscopy (cv-aas). fish were collected from vistula river at lower course and dead vistula river channel in south of baltic sea in poland. the fish species examined include round goby (neogobius melanostomus), crucian carp (carassius carassius), bull-rout ... | 2012 | 22428891 |
| [eels, carp, bream and other pyralenes ]. | | 2012 | 22364081 |
| assessment of copper and zinc levels in fish from freshwater ecosystems of moldova. | the aim of this work was to assess the levels of copper and zinc in fish from the main freshwater ecosystems of moldova, in relation with species, habitat, age, sex, season, and development stage. | 2012 | 22234853 |
| safeguarding the welfare of farmed fish at harvest. | fish welfare at harvest is easily compromised by poor choice of handling and slaughter methods, lack of attention to detail and by unnecessary adherence to fish farming traditions. the harvest process comprises fasting the fish to empty the gut, crowding the fish, gathering and moving the fish using brails, fish pumps, and sometimes also road or boat transport and finally stunning and killing the fish. the harvesting processes commonly used for bass, bream, carp, catfish, cod, eel, halibut, pang ... | 2012 | 21989953 |
| isolation and characterization of major histocompatibility class iiβ genes in an endangered north american cyprinid fish, the rio grande silvery minnow (hybognathus amarus). | the major histocompatibility complex (mhc) is a critical component of the adaptive immune response in vertebrates. due to the role that mhc plays in immunity, absence of variation within these genes may cause species to be vulnerable to emerging diseases. the freshwater fish family cyprinidae comprises the most diverse and species-rich group of freshwater fish in the world, but some are imperiled. despite considerable species richness and the long evolutionary history of the family, there are ve ... | 2011 | 21463692 |
| meiobenthos provides a food resource for young cyprinids. | young individuals of the bottom-biting (i.e. sediment-ingesting) common carp cyprinus carpio and gudgeon gobio gobio consumed significant amounts of nematodes in laboratory experiments, whereas the selective-feeding roach rutilus rutilus did not. in mesocosm enclosure experiments in the field, c. carpio strongly decreased the nematode abundance within 4 days, whereas the bottom-biting bream abramis brama did not affect the abundance until after 14 days. in controlled experiments with a known num ... | 2011 | 21235551 |
| occurrence and assessment of perfluorinated chemicals in wild fish from northern germany. | residues of the perfluorinated chemicals perfluorooctanesulfonate (pfos) and perfluorooctanoicacid (pfoa) were investigated in wild fish caught from different german waters including the north and the baltic sea, lake storkow in brandenburg, several rivers in lower saxony, and rivers and lakes in the city of berlin. the sample locations represent waters with no, low, medium and high portions of treated municipal sewage discharges. the investigated fish filet samples included 51 wild eels, 44 bre ... | 2010 | 20044123 |
| a fine structural review on the spermatozoa of cyprinidae with attention to their phylogenetic implications. | the fine structural organization and dimensions of spermatozoa from species of 4 subfamilies of the cyprinidae (barbus barbus, carassius carassius, cyprinus carpio carpio, cyprinus carpio haematopterus, abramis brama, alburnoides bipunctatus, alburnus alburnus, chalcalburnus chalcoides mento, chondrostoma nasus, hypophthalmichthys molitrix, leuciscus cephalus, phoxinus phoxinus, rutilus rutilus, rutilus meidingerii, scardinius erythrophthalmus, vimba vimba and ctenopharyngodon idella) are compar ... | 2009 | 19688692 |
| characteristics of sarcoplasmic proteins and their interaction with surimi and kamaboko gel. | this study examined the effect of adding common carp sarcoplasmic proteins (sp- p) on the gel characteristics of threadfin bream surimi and kamaboko while maintaining constant moisture and myofibrillar levels. based on the temperature sweep test, which is involved in heating of surimi gel from 10 to 80 degrees c to monitor the viscoelastic properties, at temperature range of 40 to 50 degrees c, the decrease level (depth of valley) in storage modulus (g') thermograph was in proportion to the conc ... | 2010 | 19200110 |
| single unit responses to skin odorants from conspecifics and heterospecifics in the olfactory bulb of crucian carp carassius carassius. | injured fish skin leaks alarm substances that induce the fright reaction upon olfactory detection. the skin also contains a multitude of other odorants traditionally related to other behaviors, but to what extent they are detected upon injury is unknown. we have performed single unit recordings in the olfactory bulb (ob) of crucian carp while exposing the olfactory epithelium to skin extracts from conspecifics and three other species of the carp family, common carp, tench and bream. the aims wer ... | 2008 | 18978216 |
| infection of the heart of the common bream, abramis brama (l.), with myxobolus s.l. dogieli (myxozoa, myxobolidae). | myxobolus dogieli bykhovskaya-pavlovskaya & bykhovski, 1940 is regarded as a site specific myxosporean, infecting the heart of cyprinid fish. during a survey of the myxosporean fauna of lake balaton fish, heart myxobolosis was found in the common bream, abramis brama, with heavy infection of the ventricle and the bulbus arteriosus in some infected bream. developing and mature plasmodia were mostly in the connective tissue of the subepicardium and subendocardium. plasmodia developing in the suben ... | 2008 | 18700938 |
| differential host utilisation by different life history stages of the fish ectoparasite argulus foliaceus (crustacea: branchiura). | in this study we examine differences in the occurrence of life history stages of the destructive fish ectoparasite argulus foliaceus (l., 1758) on eight fish species (stickleback, rudd, roach, gudgeon, bream, tench, crucian carp and common carp) sampled from a mixed-species recreational fishing lake on nine occasions during late spring and summer. total numbers ofa. foliaceus, as well as the number of larval, juvenile and adult parasite stages, from each fish were recorded along with the fish sp ... | 2008 | 18666418 |
| [cells with radiating vesicles in different fish species]. | a study was made of the ultrastructure of cells with radiating vesicles from the head kidney of different fish species: atlantic salmon salmo salar l., carp cyprinus carpio l., roach rutilus rutilus (l.), bream abramis brama (l.), high-body pickarel spicara staris (l.), long-striped wrasse symphodus tinca (l.), and from spleen of the rainbow trout salmo gairdneri richardson, and the tilapia sarotherodon mossambicus peters. a disposition of vesicles around centrioles was characteristics of all th ... | 2006 | 17147254 |
| an overview of health control in croatian aquaculture. | aquaculture is an important segment of croatian fishing industry with long tradition of carp and rainbow trout farming as well as marine aquaculture represented by shellfish (flat oyster and mussels), sea bass and sea bream cultivation and atlantic bluefin tuna fattening. the fish and shellfish diseases survey is regulated by "decree on the measures of animal health protection against infectious and parasite diseases" issued yearly by ministry of agriculture. this report derives from systematic ... | 2005 | 16244940 |
| levels and distribution of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in sediments and biota from the danube delta, romania. | concentrations of polychlorinated biphenyls (pcbs), polybrominated diphenyl ethers (pbdes) and organochlorine pesticides (ocps), such as dichlorodiphenyltrichloroethane (ddt) and analogues, hexachlorocyclohexanes (hchs) and hexachlorobenzene (hcb), were measured in sediments and biota (invertebrates, 11 fish species and cormorant tissues) collected in 2001 from the danube delta, the biggest european wetland. ddts were the predominant pollutants in all samples. a high variability in the concentra ... | 2006 | 16112310 |
| evaluation of pesticides and metals in fish of the dniester river, moldova. | the dniester river is one of the largest rivers in eastern europe and receives input from numerous anthropogenic activities. in october 2001, fish samples (abramis brama, rutilus rutilus heckeli, perca fluviatilis, chondrostoma nasus, carassius auratus gibelio, and cyprinus carpio) were collected and analyzed for metals and various pesticides. maximum total ddt pesticide concentrations were between 27.7 and 34.2 ng/g wet weight in muscles and gonads, respectively. dde isomers comprised up to 70% ... | 2005 | 15914239 |
| [contamination source identification study of organochlorines pesticides in 2000 chinese total diet study]. | the third chinese total diet study to estimate dietary intake of contaminants was carried out in 2000. the levels of hch and ddt residues were less significant in most of foodstuffs, but the level of hch residues of aquatic foods in south 2 and in north 1 is respectively 75.99microg/kg and 170.72microg/kg, and the ratio of gamma-hch is 90% of the total hchs residues. to identify the contamination source of gamma-hch, the individual province composite samples and the individual samples of aquatic ... | 2004 | 15461282 |
| effects of natural and synthetic estrogens and various environmental contaminants on vitellogenesis in fish primary hepatocytes: comparison of bream (abramis brama) and carp (cyprinus carpio). | interaction of environmental estrogens with the estrogen receptor (er) has been shown in various fish species. our objective was to compare the sensitivity of bream (abramis brama) to (xeno-)estrogens with that of the carp (cyprinus carpio), by measuring the effects of 17beta-estradiol (e2), estrone (e1), ethynylestradiol (ee2), bisphenol a (bpa), nonylphenol (np), methoxychlor (mxcl), and halogenated aromatic hydrocarbons (hahs) such as polychlorinated biphenyls (pcb126, pcb118), 2,3,7,8-tetrac ... | 2004 | 15159526 |
| production of transgenic silver sea bream (sparus sarba) by different gene transfer methods. | we have been interested in developing convenient mass gene transfer methods for producing strains of silver sea bream (sparus sarba) with superior genetic traits for aquaculture. a transgene construct carrying rainbow trout growth hormone (rtgh) complementary dna driven by a common carp b-actin promoter was introduced into silver sea bream by electroporating the sperm with the rtgh transgene and using the treated sperm to fertilize eggs stripped from mature females. the presence of the gh transg ... | 2002 | 14961266 |
| acetylcholinesterase and butyrylcholinesterase activities in brain and plasma of freshwater teleosts: cross-species and cross-family differences. | brain and plasma acetylcholinesterase (ache; ec 3.1.1.7) and plasma butyrylcholinesterase (bche; ec 3.1.1.8) specific activities were assayed in 16 freshwater teleosts belonging to four families: cyprinidae, percidae, esocidae and lotidae. brain ache activity varied among fish species approximately 15-fold, ranging from 138 to 2011 micromol/g per h. all cyprinids had higher brain ache activity than other fish families. plasma ache activity was on average 100-fold lower than that in brain, varyin ... | 2003 | 12781973 |
| amylolytic activity in fish intestinal mucosa: temperature effects. | the activity, temperature characteristics and energy of activation of amylolytic enzymes in the intestinal mucosa were studied in six species of fish living in a boreal zone [burbot (lota lota l.), northern pike (exos lucius l.), perch (perca fluviatilis l.), bream (abramis brama l.), roach (rutilis rutilis l.), and carp (cyprinus carpio l.)] and in three species from tropical and subtropical areas [pilchard (sardina pilchardus w.), jack mackerel (trachurus trecae c.) and round sardinella (sardi ... | 2003 | 12628384 |
| electrophoretic determination of estrogen-induced protein in fish exposed to synthetic and naturally occurring chemicals. | in a first step we investigated whether leuciscus idus, a fish routinely used to assess the quality of surface water in germany, is suitable to indicate the presence of endocrine-disrupting chemicals. an electrophoretic method was used to determine estrogen-induced protein in fish serum (presumed vitellogenin). this method was tested by exposing juvenile l. idus to 17 alpha-ethinylestradiol, 17 beta-estradiol and estrone. nominal concentrations of the steroid were compared with concentrations de ... | 1999 | 10492896 |
| in vitro effects of cadmium and ddvp (dichlorvos) on intestinal carbohydrase and protease activities in freshwater teleosts. | in vitro effects of cadmium (0.5-50 mg/l) and ddvp (0.2-100 mg/l) on the total amylolytic, sucrase and protease activities of intestinal mucosa have been studied for the first time in 11 freshwater teleosts. total amylolytic activity in burbot, crucian carp and common carp, sucrase activity in blue bream and total proteolytic activity in burbot and pike were significantly decreased by cadmium at 50 mg/l. ddvp (at 0.2 mg/l) caused a significant decrease in total proteolytic activity in pike but h ... | 1999 | 10190024 |
| acute effect of organotin compounds to red sea bream and red carp using biological parameters. | | 1996 | 8661861 |
| [the characteristics of carbohydrate transport in the intestines of fresh-water fishes]. | the maximal level of carbohydrate transport was found in the proximal portion of the intestine and in the distal one in the bream and carp. after 48-hr starvation the carbohydrate transport was decreased along the whole length of the intestine. anoxia and some inhibiting agents changed the intensity of glucose and galactose transport in the fish intestine. | 1992 | 1335912 |
| retinal morphology of cyprinid fishes: a quantitative histological study of ontogenetic changes and interspecific variation. | morphological patterns of the retina, cone size and density, rod density, rod-cone ratio, ganglion cell density, convergence of receptor cells, resolving power (rp) and regionalization were examined throughout life history in roach and in adults of asp, bream, common carp, roach and sabre carp. the retina of hatchlings is packed with small cones. during larval and juvenile growth the retina stretches, cones increase in diameter and rods are present in increasing numbers. photopic and scotopic se ... | 1991 | 1843750 |
| the development of a radioimmunoassay for carp, cyprinus carpio, vitellogenin. | the development of an easily - performed and robust radioimmunoassay (ria) to carp, cyprinus carpio, vitellogenin (c-vtg) is described. purified c-vtg was iodinated using iodogen. the resulting c-vtg label was useful for up to 60 days. high titre antibodies were raised in rabbits to the purified c-vtg. the practical operating range of the c-vtg ria was between 2 and 200 ng. ml(-1). vtg was detected in plasma from all female mirror carp investigated, and all plasmas diluted parallel to the standa ... | 1990 | 24221946 |
| brain growth patterns in four european cyprinid fish species (cyprinidae, teleostei): roach (rutilus rutilus), bream (abramis brama), common carp (cyprinus carpio) and sabre carp (pelecus cultratus). | this study compares brain growth in 4 species of cyprinids, each distinctly different in adult brain morphology: roach have generalized brains; bream are characterized by well-developed visual, octavolateralis and gustatory brain regions; common carp show chemosensory (gustatory)-dominated brains, and sabre carp octavolateralis-dominated brains. the growth patterns of 16 regions relative to total brain volume were investigated by computer-aided quantitative histology to illustrate internal brain ... | 1990 | 2379081 |
| enzymatic properties of fish muscle aldolase. | 1. aldolases were isolated from the ordinary muscle of red sea bream pagrus major, pacific mackerel scomber japonicus, and carp cyprinus carpio by ammonium sulfate fractionation, followed by ion-exchange chromatography on deae-cellulose and cm-sepharose cl-6b columns, and examined for enzymatic properties. 2. the aldolases showed the highest activity in a ph range from 6.8-7.8 km values for fructose-1,6-bisphosphate ranged from 0.025-0.10 mm. 3. irrespective of fish species, aldolase activity wa ... | 1989 | 2924547 |
| enzymatic properties of glyceraldehyde-3-phosphate dehydrogenase from fish muscle. | 1. glyceraldehyde-3-phosphate dehydrogenase was isolated from the ordinary muscle of red sea bream pagrus major, pacific mackerel scomber japonicus and carp cyprinus carpio by ammonium sulfate fractionation, followed by deae-sepharose cl-6b and deae-cellulose column chromatography and sephadex g-150 gel filtration, and examined for enzymatic properties. 2. their optimum ph values in the backward reaction ranged from 7.8 to 8.2, and km values from 1.56 to 1.90 mm. 3. irrespective of the species o ... | 1989 | 2776431 |
| the biotransformation of three 14c-labelled phenolic compounds in twelve species of freshwater fish. | 1. the urinary and biliary excretion of 14c-labelled m-cresol, 1-naphthol and o-chlorophenol were investigated in 12 species of freshwater fish (bitterling, rhodeus sericeus amarus; bream, abramis brama; crucian carp, carassius carassius; goldfish, carassius auratus; gudgeon, gobio gobio; guppy, poecilia reticulata; minnow, phoximus phoximus; perch, perca fluviatilis; roach, rutilus rutilus; rudd, scardinius erythropthalmus; three-spined stickleback, gasterosteus aculeatus; tench, tinca tinca) w ... | 1983 | 6880238 |