| the application of arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes. | the research demonstrated that arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. arabidopsis has been established as an important model system for studying plant biology and plant-microbe interactions. we show that this plant can also be used for studying the tripartite interactions among plants, the root-knot nematode meloidogyne i ... | 2015 | 25548000 |
| comparative study of the bioconversion process using r-(+)- and s-(-)-limonene as substrates for fusarium oxysporum 152b. | this study compared the bioconversion process of s-(-)-limonene into limonene-1,2-diol with the already established biotransformation of r-(+)-limonene into α-terpineol using the same biocatalyst in both processes, fusarium oxysporum 152b. the bioconversion of the s-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. when submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limone ... | 2015 | 25529726 |
| [allelopathic effects of phenolic compounds of melon root exudates on fusarium oxysporum f. sp. melonis]. | in this study, the phenolic compounds of melon root exudates were identified by hplc and seven phenolic compounds including gallic acid, phthalic acid, syringic acid, salicylic acid, ferulic acid, benzoic acid and cinnamic acid were observed. the laboratory experiment showed that ferulic acid, benzoic acid and cinnamic acid of 0.1 and 0.25 mmol x l(-1) could significantly promote the germination of fusarium oxysporum f. sp. melonis spore while salicylic acid inhibited the spore germination to so ... | 2014 | 25509089 |
| shifts in soil chemical properties and bacterial communities responding to biotransformed dry olive residue used as organic amendment. | dry olive residue (dor) is a waste product derived from olive oil extraction and has been proposed as an organic amendment. however, it has been demonstrated that a pre-treatment, such as its transformation by saprophytic fungi, is required before dor soil application. a greenhouse experiment was designed where 0 and 50 g kg(-1) of raw dor (dor), coriolopsis floccosa-transformed dor (cordor) and fusarium oxysporum-transformed dor (fusdor) were added to soil. analyses of the soil chemical propert ... | 2015 | 25501891 |
| molecular defense responses in roots and the rhizosphere against fusarium oxysporum. | plants face many different concurrent and consecutive abiotic and biotic stresses during their lifetime. roots can be infected by numerous pathogens and parasitic organisms. unlike foliar pathogens, root pathogens have not been explored enough to fully understand root-pathogen interactions and the underlying mechanism of defense and resistance. pr gene expression, structural responses, secondary metabolite and root exudate production, as well as the recruitment of plant defense-assisting "soldie ... | 2014 | 25482759 |
| hormonal regulation of gummosis and composition of gums from bulbs of hyacinth (hyacinthus orientalis). | hyacinth (hyacinthus orientalis) bulbs infected by fusarium oxysporum showed the symptoms of gummosis. the purpose of this study was to clarify the hormonal regulation of gummosis and composition of gums from hyacinth bulbs. the application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at 2% (w/w, in lanolin) induced gummosis in hyacinth bulbs. methyl jasmonate (ja-me) at 1.5% (w/w, in lanolin) induced gummosis as well. simultaneous application of ja-me and ethephon ... | 2015 | 25462960 |
| response of nbs encoding resistance genes linked to both heat and fungal stress in brassica oleracea. | environmental stresses, including both abiotic and biotic stresses, cause considerable yield loss in crops and can significantly affect their development. under field conditions, crops are exposed to a variety of concurrent stresses. among abiotic and biotic stresses, heat and fusarium oxysporum, are the most important factors affecting development and yield productivity of brassica oleracea. genes encoding the nucleotide-binding site (nbs) motif are known to be related to responses to abiotic a ... | 2015 | 25461701 |
| arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using hplc-hg-afs and in-situ xanes. | microorganisms dominated speciation of arsenic (as) play an important role in the biogeochemical cycling of as. in the study, species transformation of arsenite [as(iii)] and as(iii) influx and efflux across the cell membranes of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 cells were studied using a cellular lysis plus chromatographic separation method and further the in-situ x-ray absorption near edge structure (xanes) analysis. the results in ... | 2015 | 25460757 |
| proteomics of fusarium oxysporum race 1 and race 4 reveals enzymes involved in carbohydrate metabolism and ion transport that might play important roles in banana fusarium wilt. | banana fusarium wilt is a soil-spread fungal disease caused by fusarium oxysporum. in china, the main virulence fungi in banana are f. oxysporum race 1 (f1, weak virulence) and race 4 (f4, strong virulence). to date, no proteomic analyses have compared the two races, but the difference in virulence between f1 and f4 might result from their differentially expressed proteins. here we report the first comparative proteomics of f1 and f4 cultured under various conditions, and finally identify 99 pro ... | 2014 | 25460190 |
| lignin and lignans in plant defence: insight from expression profiling of cinnamyl alcohol dehydrogenase genes during development and following fungal infection in populus. | cinnamyl alcohol dehydrogenase (cad) catalyses the final step in the biosynthesis of monolignol, the main component of lignin. lignins, deposited in the secondary cell wall, play a role in plant defence against pathogens. we re-analysed the phylogeny of cad/cad-like genes using sequences from recently sequenced genomes, and analysed the temporal and spatial expression profiles of cad/cad-like genes in populus trichocarpa healthy and infected plants. three fungal pathogens (rhizoctonia solani, fu ... | 2014 | 25443838 |
| challenges in ethanol production with fusarium oxysporum through consolidated bioprocessing. | fusarium oxysporum has been reported as being able to both produce the enzymes necessary to degrade lignocellulosic biomass to sugars and also ferment the monosaccharides to ethanol under anaerobic or microaerobic conditions. however, in order to become an economically feasible alternative to other ethanol-producing microorganisms, a better understanding of its physiology, metabolic pathways, and bottlenecks is required, together with an improvement in its efficiency and robustness. in this repo ... | 2014 | 25424444 |
| genetic and pathogenic variability of fusarium oxysporum f. sp. cepae isolated from onion and welsh onion in japan. | fusarium oxysporum f. sp. cepae causes fusarium basal rot in onion (common onion) and fusarium wilt in welsh onion. although these diseases have been detected in various areas in japan, knowledge about the genetic and pathogenic variability of f. oxysporum f. sp. cepae is very limited. in this study, f. oxysporum f. sp. cepae was isolated from onion and welsh onion grown in 12 locations in japan, and a total of 55 f. oxysporum f. sp. cepae isolates (27 from onion and 28 from welsh onion) were ch ... | 2015 | 25412011 |
| dynamics of the establishment of multinucleate compartments in fusarium oxysporum. | nuclear dynamics can vary widely between fungal species and between stages of development of fungal colonies. here we compared nuclear dynamics and mitotic patterns between germlings and mature hyphae in fusarium oxysporum. using fluorescently labeled nuclei and live-cell imaging, we show that f. oxysporum is subject to a developmental transition from a uninucleate to a multinucleate state after completion of colony initiation. we observed a special type of hypha that exhibits a higher growth ra ... | 2015 | 25398376 |
| different continuous cropping spans significantly affect microbial community membership and structure in a vanilla-grown soil as revealed by deep pyrosequencing. | in the present study, soil bacterial and fungal communities across vanilla continuous cropping time-series fields were assessed through deep pyrosequencing of 16s ribosomal rna (rrna) genes and internal transcribed spacer (its) regions. the results demonstrated that the long-term monoculture of vanilla significantly altered soil microbial communities. soil fungal diversity index increased with consecutive cropping years, whereas soil bacterial diversity was relatively stable. bray-curtis dissimi ... | 2015 | 25391237 |
| biocontrol of fusarium wilt disease in tomato by paenibacillus ehimensis kwn38. | this study was conducted to investigate biocontrol potential of paenibacillus ehimensis kwn38 against fusarium oxysporum f.sp. lycopersici causing fusarium wilt disease in tomato. our result showed that p. ehimensis kwn38 produced extracellular organic compounds and crude enzyme to inhibit f. oxysporum f.sp. lycopersici conidial germination in in vitro assays. tomato seedlings were treated with water (w), grass medium (g), g with p. ehimensis kwn38 inoculation (gp) and g along with synthetic fun ... | 2015 | 25384610 |
| onychomycosis by fusarium oxysporum probably acquired in utero. | fusarium oxysporum has been described as a pathogen causing onychomycosis, its incidence has been increasing in immunocompetent and disseminated infection can occur in immunosuppressed individuals. we describe the first case of congenital onychomycosis in a child caused by fusarium oxysporum. the infection being acquired in utero was proven by molecular methods with the identification of the fungus both in the nail and placenta, most probably as an ascending contamination/infection in a hiv-posi ... | 2014 | 25383318 |
| the transmembrane protein sho1 cooperates with the mucin msb2 to regulate invasive growth and plant infection in fusarium oxysporum. | in the vascular wilt pathogen fusarium oxysporum, the mitogen-activated protein kinase (mapk) fmk1 is essential for plant infection. the mucin-like membrane protein msb2 regulates a subset of fmk1-dependent functions. here, we examined the role of the tetraspan transmembrane protein sho1 as an additional regulator of the fmk1 pathway and determined its genetic interaction with msb2. targeted δsho1 mutants were generated in wild-type and δmsb2 backgrounds to test possible interactions between the ... | 2015 | 25382187 |
| phenyl derivative of pyranocoumarin precludes fusarium oxysporum f.sp. lycopersici infection in lycopersicon esculentum via induction of enzymes of the phenylpropanoid pathway. | binding of phenyl derivative of pyranocoumarin (pdp) modulated activity of fungal endopolygalacturonase in silico. induced fit docking study of pdp with endopolygalacturonase (1hg8) showed a bifurcated hydrogen bond interaction with the protein at lys 244 with a docking score of -3.6 and glide energy of -37.30 kcal/mol. docking with endopolygalacturonase ii (1czf) resulted hydrogen bond formation with lys 258 with a docking score of -2.3 and glide energy of -30.42 kcal/mol. it was hypothesized t ... | 2015 | 25374140 |
| endophytic bacteria from banana cultivars and their antifungal activity. | endophytic microorganisms consist of fungi, bacteria, and actinomycetes that play important roles in the process of plant adaptation to the environment. currently, the natural associations between microorganisms and plant species are being explored for a large number of biotechnological applications. in this study, 122 endophytic bacteria were isolated from 5 cultivars of musa spp from the state of amazonas (brazil). four strains were selected because they exhibited antagonistic activities again ... | 2014 | 25366756 |
| gtpase rho1 regulates the expression of xyl3 and laccase genes in fusarium oxysporum. | the rho1 protein is a gtpase that participates in cell wall biogenesis. we analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional rho1, and rho1::hyg + rho1 (g14v) that has a constitutively active rho1. the transcript levels of lcc2, lcc3, lcc5, and xyl3 differed among the three strains, but those of lcc1 and lcc9 ... | 2015 | 25351810 |
| [faba bean fusarium wilt (fusarium oxysporum )control and its mechanism in different wheat varieties and faba bean intercropping system]. | field experiment and hydroponic culture were conducted to investigate effects of three wheat varieties (yunmai 42, yunmai 47 and mianyang 29) and faba bean intercropping on the shoot biomass, disease index of fusarium wilt, functional diversity of microbial community and the amount of fusarium oxysporum in rhizosphere of faba bean. contents and components of the soluble sugars, free amino acids and organic acids in the root exudates were also examined. results showed that, compared with monocrop ... | 2014 | 25345048 |
| highly diverse endophytic and soil fusarium oxysporum populations associated with field-grown tomato plants. | the diversity and genetic differentiation of populations of fusarium oxysporum associated with tomato fields, both endophytes obtained from tomato plants and isolates obtained from soil surrounding the sampled plants, were investigated. a total of 609 isolates of f. oxysporum were obtained, 295 isolates from a total of 32 asymptomatic tomato plants in two fields and 314 isolates from eight soil cores sampled from the area surrounding the plants. included in this total were 112 isolates from the ... | 2015 | 25304514 |
| construction of cdna expression library of watermelon for isolation of clwrky1 transcription factors gene involved in resistance to fusarium wilt. | full-length cdnas are very important for genome annotation and functional analysis of genes. the number of full-length cdnas from watermelon remains limited. here we report first the construction of a full-length enriched cdna library from fusarium wilt stressed watermelon (citrullus lanatus thunb.) cultivar pi296341 root tissues using the smart method. the titer of primary cdna library and amplified library was 2.21 x 10(6) and 2.0 x 10(10) pfu/ml, respectively and the rate of recombinant was a ... | 2014 | 25296501 |
| identification and characterization of wilt and salt stress-responsive micrornas in chickpea through high-throughput sequencing. | chickpea (cicer arietinum) is the second most widely grown legume worldwide and is the most important pulse crop in the indian subcontinent. chickpea productivity is adversely affected by a large number of biotic and abiotic stresses. micrornas (mirnas) have been implicated in the regulation of plant responses to several biotic and abiotic stresses. this study is the first attempt to identify chickpea mirnas that are associated with biotic and abiotic stresses. the wilt infection that is caused ... | 2014 | 25295754 |
| root rot of balloon flower (platycodon grandiflorum) caused by fusarium solani and fusarium oxysporum. | balloon flower (platycodon grandiflorum) is a kind of mountain herbs whose roots have restorative properties and the cultivating acreage of balloon flower has been steadily increasing in korea. more frequent rain and high amount of rainfalls as a result of climate changes predisposed balloon flower to the outbreaks of root rot at high-density cultivation area in recent years. root crowns were usually discolored into brown to blackish brown at first and the infected plants showed slight wilting s ... | 2013 | 25288973 |
| activation of salicylic acid metabolism and signal transduction can enhance resistance to fusarium wilt in banana (musa acuminata l. aaa group, cv. cavendish). | fusarium wilt caused by the fungus fusarium oxysporum f. sp. cubens (foc) is the most serious disease that attacks banana plants. salicylic acid (sa) can play a key role in plant-microbe interactions. our study is the first to examine the role of sa in conferring resistance to foc tr4 in banana (musa acuminata l. aaa group, cv. cavendish), which is the greatest commercial importance cultivar in musa. we used quantitative real-time reverse polymerase chain reaction (qrt-pcr) to analyze the expres ... | 2015 | 25277445 |
| the transcription factor con7-1 is a master regulator of morphogenesis and virulence in fusarium oxysporum. | previous studies have demonstrated the essential role of morphogenetic regulation in fusarium oxysporum pathogenesis, including processes such as cell-wall biogenesis, cell division, and differentiation of infection-like structures. we identified three f. oxysporum genes encoding predicted transcription factors showing significant identities to magnaporthe oryzae con7p, con7-1, plus two identical copies of con7-2. targeted deletion of con7-1 produced nonpathogenic mutants with altered morphogene ... | 2015 | 25271883 |
| purification and characterisation of an antifungal protein, mcha-pr, from the intercellular fluid of bitter gourd (momordica charantia) leaves. | an antifungal protein, designated mcha-pr, was isolated from the intercellular fluid of bitter gourd (momordica charantia) leaves during a screen for potent antimicrobial proteins from plants. the isolation procedure involved a combination of extraction, ammonium sulphate precipitation, gel filtration on bio-gel p-6, ion exchange chromatography on cm-sephadex, an additional gel filtration on hiload 16/60 superdex 30, and finally, hplc on a source 5rpc column. matrix-assisted laser desorption/ion ... | 2015 | 25245535 |
| fungus-mediated preferential bioleaching of waste material such as fly - ash as a means of producing extracellular, protein capped, fluorescent and water soluble silica nanoparticles. | in this paper, we for the first time show the ability of the mesophilic fungus fusarium oxysporum in the bioleaching of waste material such as fly-ash for the extracellular production of highly crystalline and highly stable, protein capped, fluorescent and water soluble silica nanoparticles at ambient conditions. when the fungus fusarium oxysporum is exposed to fly-ash, it is capable of selectively leaching out silica nanoparticles of quasi-spherical morphology within 24 h of reaction. these sil ... | 2014 | 25244567 |
| fungal infections after liver transplantation: incidence and outcome. | fungal infections, although less frequent than bacterial infections, represent a severe comorbidity with an exponential increase in mortality rate in liver transplantation patients. the incidence of invasive fungal infections (ifis) after solid organ transplantation ranges from 7% to 42%, with candida spp. and aspergillus spp. as the most common pathogens. fungal infections in liver transplant recipients have been associated with poor outcome and mortality rates ranging from 65% to 90% for invas ... | 2014 | 25242777 |
| characterization of fusarium secorum, a new species causing fusarium yellowing decline of sugar beet in north central usa. | this study characterized a novel sugar beet (beta vulgaris l.) pathogen from the red river valley in north central usa, which was formally named fusarium secorum. molecular phylogenetic analyses of three loci (translation elongation factor1α, calmodulin, mitochondrial small subunit) and phenotypic data strongly supported the inclusion of f. secorum in the fusarium fujikuroi species complex (ffsc). phylogenetic analyses identified f. secorum as a sister taxon of f. acutatum and a member of the af ... | 2014 | 25209635 |
| rapid and quantitative detection of fusarium oxysporum f. sp. cubense race 4 in soil by real-time fluorescence loop-mediated isothermal amplification. | in this study, a real-time fluorescence loop-mediated isothermal amplification (realamp) was developed and evaluated for the rapid and quantitative detection of fusarium oxysporum f. sp. cubense race 4 (r4) in soil. | 2014 | 25200557 |
| [biodegradation of agricultural plant residues by fusarium oxysporum strains]. | the cellulolytic and endoglucanase activity of fusarium oxysporum strains isolated from soil and plants in the media with plant waste as carbon source has been studied. it was established that the majority of studied strains were able to hydrolyze the filter paper, husk of sunflower seeds, wheat straw and corn stalks. cellulolytic activity depended on the strain of microscopic fungi, type of substrate and duration of cultivation. the maximum cellulase activity 1 u/ml and the concentration of red ... | 2014 | 25199344 |
| phylogeny and pathogenicity of fusarium spp. isolated from greenhouse melon soil in liaoning province. | fungi of the fusarium oxysporum are widely distributed around the world in all types of soils, and they are all anamorphic species. in order to investigate the relationships and differences among fusarium spp., 25 fusarium spp. were isolated from greenhouse melon soils in liaoning province, china. with these 25 strains, three positive control fusarium strains were analyzed using universally primed pcr (up-pcr). seventy-three bands appeared after amplification using 6 primers, and 66 of these ban ... | 2014 | 25183948 |
| effects of endogenous signals and fusarium oxysporum on the mechanism regulating genistein synthesis and accumulation in yellow lupine and their impact on plant cell cytoskeleton. | the aim of the study was to examine cross-talk interactions of soluble sugars (sucrose, glucose and fructose) and infection caused by fusarium oxysporum f.sp. lupini on the synthesis of genistein in embryo axes of lupinus luteus l.cv. juno. genistein is a free aglycone, highly reactive and with the potential to inhibit fungal infection and development of plant diseases. as signal molecules, sugars strongly stimulated accumulation of isoflavones, including genistein, and the expression of the iso ... | 2014 | 25178062 |
| in vitro inhibition activity of essential oils from some lamiaceae species against phytopathogenic fungi. | natural products have been in focus as alternative, effective and safe materials against the phytopathogens. investigations show nepeta oils as effective in controlling the food crops decay. the inhibitory effects of essential oils derived from nepeta leucophylla, nepeta ciliaris, nepeta clarkei and calamintha umbrosa against five phytopthogenic fungi have been determined. in vitro antifungal activity varied with their constituents and target species. more active being the oils containing oxygen ... | 2014 | 25175652 |
| tomato susceptibility to fusarium crown and root rot: effect of grafting combination and proteomic analysis of tolerance expression in the rootstock. | grafting can enhance the tolerance of vegetable crops to soilborne diseases. the aim of this study was to investigate whether different tomato scion-rootstock combinations may affect the plant susceptibility to fusarium oxysporum f. sp. radicis-lycopersici (forl), the causal agent of crown and root rot. a proteomic approach was used to investigate whenever the protein repertoire of the rootstock can be modified by forl infection, in relation to cultivar susceptibility/tolerance to the disease. t ... | 2014 | 25173633 |
| characterization of fusarium wilt resistant somaclonal variants of banana cv. rasthali by cdna-rapd. | fusarium wilt of banana, caused by fusarium oxysporum f. sp. cubense (foc), is counted among the most destructive diseases of crop plants in india. in the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop p ... | 2014 | 25160909 |
| changes induced by trichoderma harzianum in suppressive compost controlling fusarium wilt. | the addition of species of trichoderma to compost is a widespread technique used to control different plant diseases. the biological control activity of these species is mainly attributable to a combination of several mechanisms of action, which may affect the microbiota involved in the suppressiveness of compost. this study was therefore performed to determine the effect of inoculation of trichoderma harzianum (t. harzianum) on compost, focusing on bacterial community structure (16s rrna) and c ... | 2013 | 25149244 |
| identification and expression analysis of wrky family genes under biotic and abiotic stresses in brassica rapa. | wrky proteins constitute one of the largest transcription factor families in higher plants, and they are involved in multiple biological processes such as plant development, metabolism, and responses to biotic and abiotic stresses. genes of this family have been well documented in response to many abiotic and biotic stresses in many plant species, but not yet against pectobacterium carotovorum subsp. carotovorum and fusarium oxysporum f.sp. conglutinans in any of the plants. moreover, potentiali ... | 2015 | 25149146 |
| exploring microrna-like small rnas in the filamentous fungus fusarium oxysporum. | rna silencing such as quelling and meiotic silencing by unpaired dna (msud) and several other classes of special small rnas have been discovered in filamentous fungi recently. more than four different mechanisms of microrna-like rnas (milrnas) production have been illustrated in the model fungus neurospora crassa including a dicer-independent pathway. to date, very little work focusing on small rnas in fungi has been reported and no universal or particular characteristic of milrnas were defined ... | 2014 | 25141304 |
| fusarium oxysporum induces the production of proteins and volatile organic compounds by trichoderma harzianum t-e5. | trichoderma species have been used widely as biocontrol agents for the suppression of soil-borne pathogens. however, some antagonistic mechanisms of trichoderma are not well characterized. in this study, a series of laboratory experiments were designed to characterize the importance of mycoparasitism, exoenzymes, and volatile organic compounds (vocs) by trichoderma harzianum t-e5 for the control of fusarium oxysporum f. sp. cucumerinum (foc). we further tested whether these mechanisms were induc ... | 2014 | 25135494 |
| extracellular chitin deacetylase production in solid state fermentation by native soil isolates of penicillium monoverticillium and fusarium oxysporum. | extracellular chitin deacetylase production by native soil isolates of penicillium monoverticillium cfr 2 and fusarium oxysporum cfr 8 in solid state fermentation (ssf) using commercial wheat bran (cwb) and shrimp processing by-products (spp) as solid substrate has been studied. p. monoverticillium produced maximum chitin deacetylase activity of 547.7 ± 45 and 390.2 ± 31 units/g initial dry substrate (u/g ids) at 96 h of incubation in cwb and spp media, respectively. while, f. oxysporum produced ... | 2014 | 25114353 |
| a major qtl associated with fusarium oxysporum race 1 resistance identified in genetic populations derived from closely related watermelon lines using selective genotyping and genotyping-by-sequencing for snp discovery. | a major quantitative trait locus (qtl) for fusarium oxysporum fr. f. sp. niveum race 1 resistance was identified by employing a "selective genotyping" approach together with genotyping-by-sequencing technology to identify qtls and single nucleotide polymorphisms associated with the resistance among closely related watermelon genotypes. fusarium wilt is a major disease of watermelon caused by the soil-borne fungus fusarium oxysporum schlechtend.:fr. f. sp. niveum (e.f. sm.) w.c. snyder & h.n. han ... | 2014 | 25104326 |
| benzimidazole-1,2,3-triazole hybrid molecules: synthesis and evaluation for antibacterial/antifungal activity. | a novel series of hybrid molecules 4a-i and 5a-i were prepared by condensation of 4-(trimethylsilylethynyl)benzaldehyde 1 with substituted o-phenylenediamines. these in turn were reacted with 2-(azidomethoxy)ethyl acetate in a cu alkyne-azide cycloaddition (cuaac) to generate the 1,2,3-triazole pharmacophore under microwave assistance. the newly synthesized compounds were examined for their in vitro antimicrobial activities against gram-positive and gram-negative bacteria and the phytopathogenic ... | 2014 | 25088180 |
| genome sequence of fusarium oxysporum f. sp. melonis strain nrrl 26406, a fungus causing wilt disease on melon. | horizontal chromosome transfer introduces host-specific pathogenicity among members of the fusarium oxysporum species complex and is responsible for some of the most destructive and intractable plant diseases. this paper reports the genome sequence of f. oxysporum f. sp. melonis (nrrl 26406), a causal agent of fusarium wilt disease on melon. | 2014 | 25081257 |
| screening of endophytic bacteria against fungal plant pathogens. | bacterial endophytes were found from 6 plant leaves among 35 plant leaves screened. two of the isolated bacteria showed antagonistic activity against fungal plant pathogens. an isolate named kl1 showed the clear inihibition against plant pathogens, fusarium oxysporum and rhizoctonia solani, on pda as well as tsa plate. supernatant of the bacterial culture also showed the clear inhibition against the fungal growth on the plate and the antibiotic substance was identified as iturin a by hplc analys ... | 2013 | 25078813 |
| molecular determination of mixed infections of dermatophytes and nondermatophyte molds in individuals with onychomycosis. | reports of mixed infections with nondermatophyte molds (ndms) and dermatophytes in onychomycosis are rare, possibly owing to the inhibition of ndm growth during traditional culture. we sought to determine the prevalence of mixed infections in onychomycosis using molecular identification. | 2014 | 25076075 |
| identification of predictor parameters to determine agro-industrial compost suppressiveness against fusarium oxysporum and phytophthora capsici diseases in muskmelon and pepper seedlings. | the lack of reliable prediction tools for evaluation of the level and specificity of compost suppressiveness limits its application. in our study, different chemical, biological and microbiological parameters were used to evaluate their potential use as a predictor parameter for the suppressive effect of composts against fusarium oxysporum f. sp. melonis (fom) and phytophthora capsici (p. capsici) in muskmelon and pepper seedlings respectively. composts were obtained from artichoke sludge, chopp ... | 2015 | 25074864 |
| first report of potato stem-end rot caused by fusarium oxysporum in korea. | in this study, we identified the causative agent of stem-end rot in potatoes that were grown in gangwon alpine areas of korea in 2013. the disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. the fungal species isolated from the infected potatoes were grown on potato dextrose agar and produced white aerial mycelia with dark violet pigments. the conidiophores were branched and monophialidic. the microconidia had elli ... | 2014 | 25071394 |
| biocontrol of fusarium crown and root rot and promotion of growth of tomato by paenibacillus strains isolated from soil. | in this study, bacterial strains were isolated from soils from 30 locations of samcheok, gangwon province. of the isolated strains, seven showed potential plant growth promoting and antagonistic activities. based on cultural and morphological characterization, and 16s rrna gene sequencing, these strains were identified as paenibacillus species. all seven strains produced ammonia, cellulase, hydrocyanic acid, indole-3-acetic acid, protease, phosphatase, and siderophores. they also inhibited the m ... | 2014 | 25071385 |
| siderophore as a potential plant growth-promoting agent produced by pseudomonas aeruginosa jas-25. | siderophores scavenges fe(+3) from the vicinity of the roots of plants, and thus limit the amount of iron required for the growth of pathogens such as fusarium oxysporum, pythium ultimum, and fusarium udum, which cause wilt and root rot disease in crops. the ability of pseudomonas to grow and to produce siderophore depends upon the iron content, ph, and temperature. maximum yield of siderophore of 130 μm was observed at ph 7.0 ± 0.2 and temperature of 30 °c at 30 h. the threshold level of iron w ... | 2014 | 25062779 |
| [biological characteristics of fusarium oxysporum and inhibitory effects of five fungicides]. | the mycelium growth and sporulation in different temperature, ph value and light conditions were detected by using the crossing and haemocytometer method. the toxicity of five fungicides to fusarium oxysporum was tested by mycelia growth method, so as to provide the reference for prevention and control against f. oxysporum. the optimum temperature and ph value of f. oxysporum to grow and spore were 28 degrees c and 6-7. alternating light and darkness promoted growth and sporulation of bacterial ... | 2014 | 25039169 |
| characterization of a yam class iv chitinase produced by recombinant pichia pastoris x-33. | a yam (dioscorea opposita thunb) class iv chitinase, whose genomic dna was cloned by mitsunaga et al. (2004), was produced by the recombinant pichia pastoris x-33 in high yields such as 66 mg/l of culture medium. the chitinase was purified by column chromatography after endoglycosidase h treatment and then characterized. it showed properties similar to the original chitinase e purified from the yam tuber reported by arakane et al. (2000). this pichia-produced chitinase also showed strong lytic a ... | 2014 | 25036674 |
| staurosporine from the endophytic streptomyces sp. strain cns-42 acts as a potential biocontrol agent and growth elicitor in cucumber. | chinese medicinal plants and their surrounding rhizospheric soil serve as promising sources of actinobacteria. a total of 180 actinobacteria strains were isolated from the rhizosphere soil, leaves, stems, and roots of nine selected plants and have been identified as potential biocontrol agents against fusarium oxysporum f. sp. cucumerinum. an endophytic strain cns-42 isolated from alisma orientale showed the largest zone of inhibition demonstrating a potent effect against f. oxysporum f. sp. cuc ... | 2014 | 25035061 |
| reduction of fusarium rot and maintenance of fruit quality in melon using eco-friendly hot water treatment. | significant losses in harvested fruit can be directly attributable to decay fungi and quality deterioration. hot water treatment (hwt) has been demonstrated to be an effective and economic environment-friendly approach for managing postharvest decay and maintaining fruit quality. in this study, the effects of hwt (45 °c for 10, 15, 20, and 25 min) on in vitro growth of fusarium oxysporum, in vivo fusarium rot, and natural decay of melon were investigated. hwt inhibited spore germination and germ ... | 2014 | 25030787 |
| toxic influence of silver and uranium salts on activated sludge of wastewater treatment plants and synthetic activated sludge associates modeled on its pure cultures. | toxic impact of silver and uranium salts on activated sludge of wastewater treatment facilities has been studied. some dominating cultures (an active nitrogen fixer agrobacterium tumifaciens (a.t) and micromyces such as fusarium nivale, fusarium oxysporum, and penicillium glabrum) have been isolated and identified as a result of selection of the activated sludge microorganisms being steadiest under stressful conditions. for these cultures, the lethal doses of silver amounted 1, 600, 50, and 300 ... | 2015 | 25027236 |
| hyphal growth of phagocytosed fusarium oxysporum causes cell lysis and death of murine macrophages. | fusarium oxysporum is an important plant pathogen and an opportunistic pathogen of humans. here we investigated phagocytosis of f. oxysporum by j774.1 murine cell line macrophages using live cell video microscopy. macrophages avidly migrated towards f. oxysporum germlings and were rapidly engulfed after cell-cell contact was established. f. oxysporum germlings continued hyphal growth after engulfment by macrophages, leading to associated macrophage lysis and escape. macrophage killing depended o ... | 2014 | 25025395 |
| phytohormone profiles induced by trichoderma isolates correspond with their biocontrol and plant growth-promoting activity on melon plants. | the application of trichoderma strains with biocontrol and plant growth-promoting capacities to plant substrates can help reduce the input of chemical pesticides and fertilizers in agriculture. some trichoderma isolates can directly affect plant pathogens, but they also are known to influence the phytohormonal network of their host plant, thus leading to an improvement of plant growth and stress tolerance. in this study, we tested whether alterations in the phytohormone signature induced by diff ... | 2014 | 25023078 |
| marker assisted selection (mas) for chickpea fusarium oxysporum wilt resistant genotypes using pcr based molecular markers. | the exploration of genetically superior accessions is the key source of germplasm conservation and potential breeding material for the future. to meet the demand of better yielding chickpea cultivars in pakistan the present study was organized to select more stable and resistant lines from indigenous as well as exotic chickpea germplasm obtained from plant genetic resource institute (pgri), national agricultural research centre, islamabad, pakistan. for the identification and evaluation of chick ... | 2014 | 25017202 |
| native cell-death genes as candidates for developing wilt resistance in transgenic banana plants. | in order to feed an ever-increasing world population, there is an urgent need to improve the production of staple food and fruit crops. the productivity of important food and fruit crops is constrained by numerous biotic and abiotic factors. the cultivation of banana, which is an important fruit crop, is severely threatened by fusarium wilt disease caused by infestation by an ascomycetes fungus fusarium oxysporum f. sp. cubense (foc). since there are no established edible cultivars of banana res ... | 2014 | 24996429 |
| genotypic and phenotypic characterization of fungi in the fusarium oxysporum species complex from soybean roots. | isolates in the fusarium oxysporum species complex (fosc) from soybean range from nonpathogenic to aggressive pathogens causing seedling damping-off, wilt, and root rot. the objective of this research was to characterize the genotype and phenotype of isolates within the fosc recovered predominantly from soybean roots and seedlings. sequence analyses of the translation elongation factor (tef1α) gene and the mitochondrial small subunit (mtssu), polymerase chain reaction restriction fragment length ... | 2014 | 24983844 |
| toxicity of abiotic stressors to fusarium species: differences in hydrogen peroxide and fungicide tolerance. | stress sensitivity of three related phytopathogenic fusarium species (fusarium graminearum, fusarium oxysporum and fusarium verticillioides) to different oxidative, osmotic, cell wall, membrane, fungicide stressors and an antifungal protein (paf) were studied in vitro. the most prominent and significant differences were found in oxidative stress tolerance: all the three f. graminearum strains showed much higher sensitivity to hydrogen peroxide and, to a lesser extent, to menadione than the other ... | 2014 | 24939687 |
| purification, characterization, and in vitro activity of 2,4-di-tert-butylphenol from pseudomonas monteilii psf84: conformational and molecular docking studies. | a novel strain of pseudomonas monteilii, psf84, was isolated from tannery waste soil from jajmau, kanpur, india. 16s rrna gene sequence phylogenetic analysis confirmed the taxonomic affiliation of psf84 as p. monteilii. an antifungal volatile organic compound (voc) active against hyphal growth of fusarium oxysporum (cimap-imi-357464) in vitro was isolated from strain psf84 by using chromatographic techniques. the molecular formula of the antifungal voc was deduced to be c₁₄h₂₂o by ei-ms and 1d a ... | 2014 | 24934765 |
| effect of physicochemical parameters on nitrile-hydrolyzing potentials of newly isolated nitrilase of fusarium oxysporum f. sp. lycopercisi ed-3. | in recent years, nitrilases from fungus have received increasing attention, and most of the studies are performed on nitrilases of bacterial origin. frequently used methods are based on analytical methods such as high-performance liquid chromatography, liquid chromatography-mass spectrometry, and gas chromatography; therefore, an efficient, user friendly, and rapid method has been developed to screen nitrilase enzyme based on the principle of color change of a ph indicator. phenol red amended wi ... | 2015 | 24923632 |
| development of dart markers and assessment of diversity in fusarium oxysporum f. sp. ciceris, wilt pathogen of chickpea (cicer arietinum l.). | fusarium oxysporum f. sp. ciceris (foc), the causal agent of fusarium wilt of chickpea is highly variable and frequent recurrence of virulent forms have affected chickpea production and exhausted valuable genetic resources. the severity and yield losses of fusarium wilt differ from place to place owing to existence of physiological races among isolates. diversity study of fungal population associated with a disease plays a major role in understanding and devising better disease control strategie ... | 2014 | 24912854 |
| non-thermal plasma treatment diminishes fungal viability and up-regulates resistance genes in a plant host. | reactive oxygen and nitrogen species can have either harmful or beneficial effects on biological systems depending on the dose administered and the species of organism exposed, suggesting that application of reactive species can possibly produce contradictory effects in disease control, pathogen inactivation and activation of host resistance. a novel technology known as atmospheric-pressure non-thermal plasma represents a means of generating various reactive species that adversely affect pathoge ... | 2014 | 24911947 |
| the tomato wilt fungus fusarium oxysporum f. sp. lycopersici shares common ancestors with nonpathogenic f. oxysporum isolated from wild tomatoes in the peruvian andes. | fusarium oxysporum is an ascomycetous fungus that is well-known as a soilborne plant pathogen. in addition, a large population of nonpathogenic f. oxysporum (npf) inhabits various environmental niches, including the phytosphere. to obtain an insight into the origin of plant pathogenic f. oxysporum, we focused on the tomato (solanum lycopersicum) and its pathogenic f. oxysporum f. sp. lycopersici (fol). we collected f. oxysporum from wild and transition solanum spp. and modern cultivars of tomato ... | 2014 | 24909710 |
| differential protein accumulations in isolates of the strawberry wilt pathogen fusarium oxysporum f. sp. fragariae differing in virulence. | this study was conducted to define differences in fusarium oxysporum f. sp. fragariae (fof) isolates with different virulence efficiency to strawberry at the proteome level, in combination with their differences in mycelial growth, conidial production and germination. comparative proteome analyses revealed substantial differences in mycelial proteomes between fof isolates, where the 54 differentially accumulated protein spots were consistently over-accumulated or exclusively in the highly virule ... | 2014 | 24907490 |
| quantum dot and superparamagnetic nanoparticle interaction with pathogenic fungi: internalization and toxicity profile. | for several years now, nanoscaled materials have been implemented in biotechnological applications related to animal (in particular human) cells and related pathologies. however, the use of nanomaterials in plant biology is far less widespread, although their application in this field could lead to the future development of plant biotechnology applications. for any practical use, it is crucial to elucidate the relationship between the nanomaterials and the target cells. in this work we have eval ... | 2014 | 24853082 |
| neuroprotective effects of bikaverin on h2o2-induced oxidative stress mediated neuronal damage in sh-sy5y cell line. | the generation of free radicals and oxidative stress has been linked to several neurodegenerative diseases including parkinson's disease, alzheimer's disease, huntington's disease, and amyotrophic lateral sclerosis. the use of free radical scavenging molecules for the reduction of intracellular reactive oxygen species is one of the strategies used in the clinical management of neurodegeneration. fungal secondary metabolism is a rich source of novel molecules with potential bioactivity. in the cu ... | 2014 | 24848007 |
| optimization and kinetic modeling of cell-associated camptothecin production from an endophytic fusarium oxysporum nfx06. | the production of cell-associated camptothecin (cpt) from an endophytic fungus fusarium oxysporum nfx06 isolated from nothapodytes foetida and its kinetics studies were proposed. response surface methodology (rsm) based on central composite design (ccd) was used to construct a model to describe the effects of substrate concentration. three independent variables (dextrose, peptone, and mgso4) were successfully employed to study the yield of cpt under submerged fermentation. the maximum yield of c ... | 2015 | 24840354 |
| characterization of anticancer, dnase and antifungal activity of pumpkin 2s albumin. | the plant 2s albumins exhibit a spectrum of biotechnologically exploitable functions. among them, pumpkin 2s albumin has been shown to possess rnase and cell-free translational inhibitory activities. the present study investigated the anticancer, dnase and antifungal activities of pumpkin 2s albumin. the protein exhibited a strong anticancer activity toward breast cancer (mcf-7), ovarian teratocarcinoma (pa-1), prostate cancer (pc-3 and du-145) and hepatocellular carcinoma (hepg2) cell lines. ac ... | 2014 | 24814706 |
| analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic fusarium oxysporum cs-20. | a defence response can be induced by nonpathogenic fusarium oxysporum cs-20 in several crops, but the molecular mechanism has not been clearly demonstrated. in the present study, we analysed the defence mechanism of a susceptible cucumber cultivar (cucumis sativus l. 9930) against a pathogen (f. oxysporum f. sp. cucumerinum) through the root precolonization of cs-20. a challenge inoculation assay indicated that the disease severity index (dsi) was reduced, ranging from 18.83 to 61.67 in comparis ... | 2014 | 24810367 |
| retransformation of marker-free potato for enhanced resistance against fungal pathogens by pyramiding chitinase and wasabi defensin genes. | multi-auto-transformation vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators and thus facilitating transgene stacking. in the study reported here, retransformation was carried out in marker-free transgenic potato cv. may queen containing chic gene (isolated from streptomyces griseus strain hut 6037) with wasabi defensin (wd) gene (isolated from wasabia japonica) to pyramid the two disease resistant g ... | 2014 | 24802621 |
| [features of interaction bacterial strains micrococcus luteus lbk1 from plants varieties/hybrids cucumber and sweet pepper and with fungus fusarium oxysporum scelecht]. | the article presents the results of studying the impact of bacterial strain m. luteus lbk1, stimulating the growth and development of plant varieties/hybrids of cucumber and sweet pepper on the intensity of sporulation of the fungus f. oxysporum scelecht--fusariose rot pathogen. | 2014 | 24800513 |
| [research wilt disease of salvia miltiorrhiza and its pathogen]. | salvia miltiorrhiza is a highly valued traditional chinese medicine for the treatment of atherosclerosis-related disorders in china, such as cardiovascular and cerebrovascular diseases in china. the wilt disease is serious in the culture of s. miltiorrhiza. wilt disease cause biomass of plant shoots and roots is lessened, active components are decreased. to solve these problems, we research the pathogen causing wilt disease of s. miltiorrhiza. the suspected pathogen is identified by morphology a ... | 2013 | 24791484 |
| increased resistance to fungal wilts in transgenic eggplant expressing alfalfa glucanase gene. | the wilt diseases caused by verticillium dahliae and fusarium oxysporum are the major diseases of eggplant (solanum melongena l.). in order to generate transgenic resistance against the wilt diseases, agrobacterium-mediated gene transfer was performed to introduce alfalfa glucanase gene encoding an acidic glucanase into eggplant using neomycin phosphotransferase (npt-ii) gene as a plant selection marker. the transgene integration into eggplant genome was confirmed by polymerase chain reaction (p ... | 2014 | 24757318 |
| a high efficiency gene disruption strategy using a positive-negative split selection marker and electroporation for fusarium oxysporum. | the fusarium oxysporum species complex consists of fungal pathogens that cause serial vascular wilt disease on more than 100 cultivated species throughout the world. gene function analysis is rapidly becoming more and more important as the whole-genome sequences of various f. oxysporum strains are being completed. gene-disruption techniques are a common molecular tool for studying gene function, yet are often a limiting step in gene function identification. in this study we have developed a f. o ... | 2014 | 24755311 |
| exserolides a-f, new isocoumarin derivatives from the plant endophytic fungus exserohilum sp. | six new isocoumarin derivatives, exserolides a-f (1-6), were isolated from solid cultures of the plant endophytic fungus exserohilum sp., together with four known metabolites (7-10). the structures of 1-6 were elucidated primarily by nmr experiments. the absolute configuration of the c-3 methine carbon in 1-5 was deduced via the circular dichroism data, whereas that of the 1,3-diol moiety in 6 was assigned from the (1)h nmr data of its (r)- and (s)-mtpa diesters. compounds 3 and 9 showed antifun ... | 2014 | 24752143 |
| genome and transcriptome analysis of the fungal pathogen fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. | the asexual fungus fusarium oxysporum f. sp. cubense (foc) causing vascular wilt disease is one of the most devastating pathogens of banana (musa spp.). to understand the molecular underpinning of pathogenicity in foc, the genomes and transcriptomes of two foc isolates were sequenced. | 2014 | 24743270 |
| genealogical concordance phylogenetic species recognition in the fusarium oxysporum species complex. | fusarium oxysporum is an important plant and human pathogenic ascomycetous group, with near ubiquity in agricultural and non-cultivated ecosystems. phylogenetic evidence suggests that f. oxysporum is a complex of multiple morphologically cryptic species. species boundaries and limits of genetic exchange within this complex are poorly defined, largely due to the absence of a sexual state and the paucity of morphological characters. this study determined species boundaries within the f. oxysporum ... | 2014 | 24742832 |
| disseminated fusarium oxysporum neurospinal infection. | we report a case of disseminated meningospondylodiscitis in an elderly diabetic patient caused by fusarium oxysporum. as the clinical presentation was nonspecific, the diagnosis of the condition could only be arrived at after laboratory and imaging studies. the diagnosis of the condition requires a high index of suspicion. patient underwent thorough surgical debridement along with a short course of variconazole and remained asymptomatic after 36 months of diagnosis. fusarium is a large genus of ... | 2014 | 24741147 |
| synthesis of gold nanoparticles from different cellular fractions of fusarium oxysporum. | the addition of varying concentrations of precursor gold salt to different cellular fractions of fusarium oxysporum, viz., the culture filtrate and the intracellular extract obtained in the growing and resting phase of the cells had a profound influence on the size, shape, and state of aggregation of the nanoparticles. multiply-twinned nanoparticles were obtained when the culture filtrate was used for synthesizing nanoparticles while mostly irregular shapes were obtained with the intracellular e ... | 2014 | 24734569 |
| growth inhibitory effects of gossypol and related compounds on fungal cotton root pathogens. | the effect of the terpenoids gossypol, 6-methoxygossypol, 6,6'-dimethoxygossypol, gossypolone and apogossypolone on growth of fungal soil pathogens was investigated. the compounds were tested at a concentration of 100 μg ml(-1) in a czapek dox agar medium at 25°c. gossypol, gossypolone and apogossypolone demonstrated strong growth inhibitory activity (≥90%) against pythium irregulare, pythium ultimum and fusarium oxysporum. these same terpenoids provided good growth inhibition against most rhizo ... | 2014 | 24713043 |
| [cutaneous infection due to fusarium oxysporum in a female diabetic: molecular biological detection of the mold from formalin-fixed paraffin embedded tissue using sequencing of the its region of the rdna]. | skin lesions due to fusarium spp. occur either secondarily following hematological spread in systemic infection or represent primary cutaneous infections following traumatic inoculation. | 2014 | 24706078 |
| identification of candidate genes for fusarium yellows resistance in chinese cabbage by differential expression analysis. | fusarium yellows caused by fusarium oxysporum f. sp. conglutinans is an important disease of brassica worldwide. to identify a resistance (r) gene against fusarium yellows in chinese cabbage (brassica rapa var. pekinensis), we analyzed differential expression at the whole genome level between resistant and susceptible inbred lines using rna sequencing. four hundred and eighteen genes were significantly differentially expressed, and these were enriched for genes involved in response to stress or ... | 2014 | 24668026 |
| genetic mapping, synteny, and physical location of two loci for fusarium oxysporum f. sp. tracheiphilum race 4 resistance in cowpea [vignaunguiculata (l.) walp]. | fusarium wilt is a vascular disease caused by the fungus fusariumoxysporum f.sp. tracheiphilum (fot) in cowpea [vignaunguiculata (l.) walp]. in this study, we mapped loci conferring resistance to fot race 4 in three cowpea ril populations: it93k-503-1 × cb46, cb27 × 24-125b-1, and cb27 × it82e-18/big buff. two independent loci which confer resistance to fot race 4 were identified, fot4-1 and fot4-2. fot4-1 was identified in the it93k-503-1 (resistant) × cb46 (susceptible) population and was posi ... | 2014 | 24659904 |
| model complexes of key intermediates in fungal cytochrome p450 nitric oxide reductase (p450nor). | denitrifying bacteria and fungi efficiently detoxify the toxic metabolite nitric oxide (no) through reduction to nitrous oxide (n2o) using nitric oxide reductase (nor) enzymes. in fungi, for example fusarium oxysporum, no is reduced by a cytochrome p450 nor (p450nor). this enzyme contains a heme b center coordinated to a proximal cysteinate ligand in the active site. in the proposed mechanism of p450nor, the ferric heme binds no first to form a ferric heme-nitrosyl complex, which is subsequently ... | 2014 | 24658055 |
| phylogenetic relationship between different race representative populations of fusarium oxysporum f. sp. ciceris in respect of translation elongation factor-1α, β-tubulin, and internal transcribed spacer region genes. | genetic diversity of 70 isolates of fusarium oxysporum f. sp. ciceris originated from various states of india representing eight races causing wilt in chickpea (cicer arietinum) was analyzed using translation elongation factor-1α (tef-1α), β-tubulin, and internal transcribed spacer (its) gene regions. tef-1α, β-tubulin, and its gene-specific markers produced ~720-, ~500-, and ~550-bp amplicons, respectively, in all the isolates of the pathogen. a phylogenetic tree constructed from the sequences ... | 2014 | 24639029 |
| antimicrobial activity and physical characterization of silver nanoparticles green synthesized using nitrate reductase from fusarium oxysporum. | nanostructures from natural sources have received major attention due to wide array of biological activities and less toxicity for humans, animals, and the environment. in the present study, silver nanoparticles were successfully synthesized using a fungal nitrate reductase, and their biological activity was assessed against human pathogenic fungi and bacteria. the enzyme was isolated from fusarium oxysporum iran 31c after culturing on malt extract-glucose-yeast extract-peptone (mgyp) medium. th ... | 2014 | 24610039 |
| studies of detailed biofilm characterization on fly ash concrete in comparison with normal and superplasticizer concrete in seawater environments. | in cooling water systems, many concrete structures in the form of tanks, pillars and reservoirs that come in contact with aggressive seawater are being deteriorated by chemical and biological factors. the nuclear industry has decided to partially replace the portland cement with appropriate pozzolans such as fly ash, which could densify the matrix and make the concrete impermeable. three types of concrete mixes, viz., normal concrete (nc), concrete with fly ash and superplasticizer (fa) and conc ... | 2014 | 24600839 |
| the pqqc gene is essential for antifungal activity of pseudomonas kilonensis jx22 against fusarium oxysporum f. sp. lycopersici. | strain jx22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing pseudomonas kilonensis. in this study, the mutant jx22mt1 was obtained by the ez-tn5 transposon mutation and showed no antifungal activity against fusarium oxysporum f. sp. lycopersici as compared with wild-type strain jx22. the pqqc gene was disrupted in the mutant. antifungal activity at the wild-type level was restored from the mutant jx22mt1 with the introduction ... | 2014 | 24588744 |
| murine model for fusarium oxysporum invasive fusariosis reveals organ-specific structures for dissemination and long-term persistence. | the soil-borne plant pathogen fusarium oxysporum causes life-threatening invasive fusariosis in immunocompromised individuals. the mechanism of infection in mammalian hosts is largely unknown. in the present study we show that the symptoms of disseminated fusariosis caused by f. oxysporum in immunosuppressed mice are remarkably similar to those reported in humans. distinct fungal structures were observed inside the host, depending on the infected organ. invasive hyphae developed in the heart and ... | 2014 | 24587124 |
| identification of the main toxins isolated from fusarium oxysporum f. sp. pisi race 2 and their relation with isolates' pathogenicity. | fusarium oxysporum f. sp. pisi (fop) is a pathogen of field pea inducing severe vascular wilt worldwide. plant resistance to races 1, 5, and 6, producing wilt symptoms, is conferred by a single dominant gene, while resistance to race 2, which gives near-wilt symptoms, have been recently showed to be quantitative. among the virulence factors reported to play a role in the infection process, toxin production is one of the best studied. thus, five race 2 isolates have been investigated for toxin pr ... | 2014 | 24568659 |
| agp2p, the plasma membrane transregulator of polyamine uptake, regulates the antifungal activities of the plant defensin nad1 and other cationic peptides. | cationic antifungal peptides (afps) act through a variety of mechanisms but share the common feature of interacting with the fungal cell surface. nad1, a defensin from nicotiana alata, has potent antifungal activity against a variety of fungi of both hyphal and yeast morphologies. the mechanism of action of nad1 occurs via three steps: binding to the fungal cell surface, permeabilization of the plasma membrane, and internalization and interaction with intracellular targets to induce fungal cell ... | 2014 | 24566173 |
| in vitro antifugal activity of medicinal plant extract against fusarium oxysporum f. sp. lycopersici race 3 the causal agent of tomato wilt. | medicinal plant extracts of five plants; adhatoda vasica, eucalyptus globulus, lantana camara, nerium oleander and ocimum basilicum collected from cairo, egypt were evaluated against fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions using water and certain organic solvents. the results revealed that cold distilled water extracts of o. basilicum and e. globulus were the most effective ones for inhibiting the growth of f. oxysporum f. sp. lycopersici. butanolic and ethanolic extract ... | 2014 | 24561899 |
| manipulating cinnamyl alcohol dehydrogenase (cad) expression in flax affects fibre composition and properties. | in recent decades cultivation of flax and its application have dramatically decreased. one of the reasons for this is unpredictable quality and properties of flax fibre, because they depend on environmental factors, retting duration and growing conditions. these factors have contribution to the fibre composition, which consists of cellulose, hemicelluloses, lignin and pectin. by far, it is largely established that in flax, lignin reduces an accessibility of enzymes either to pectin, hemicellulos ... | 2014 | 24552628 |
| functional analysis of the conserved transcriptional regulator cfwor1 in cladosporium fulvum reveals diverse roles in the virulence of plant pathogenic fungi. | fungal wor1-like proteins are conserved transcriptional regulators that are reported to regulate the virulence of several plant pathogenic fungi by affecting the expression of virulence genes. here, we report the functional analysis of cfwor1, the homologue of wor1 in cladosporium fulvum. δcfwor1 mutants produce sclerotium-like structures and rough hyphae, which are covered with a black extracellular matrix. these mutants do not sporulate and are no longer virulent on tomato. a ce.cfwor1 transfo ... | 2014 | 24521437 |
| morphological and molecular characterization of fusarium. solani and f. oxysporum associated with crown disease of oil palm. | crown disease (cd) is infecting oil palm in the early stages of the crop development. previous studies showed that fusarium species were commonly associated with cd. however, the identity of the species has not been resolved. this study was carried out to identify and characterize through morphological approaches and to determine the genetic diversity of the fusarium species. 51 isolates (39%) of fusarium solani and 40 isolates (31%) of fusarium oxysporum were recovered from oil palm with typica ... | 2013 | 24516465 |