| experimental human leprosy in the footpad of mice. | | 1966 | 5325398 |
| experimental infection of the ear and foot pad of the golden hamster with mycobacterium leprae. | | 1966 | 5325423 |
| maintenance of cytopathic activity of mycobacterium leprae in eagle's medium supplemented by mycobacterial extracts. | | 1965 | 5325956 |
| further studies on b.663 in murine leprosy. absence of resistance of m. lepraemurium to b.663 and delay in development of resistance to isoniazid. | | 1966 | 5327050 |
| lepra reaction. its relation with fragmentation of mycobacterium leprae under sulfone therapy. | | 1966 | 5327411 |
| a culture medium for the isolation of acidfast bacteria in leprous materials. | | 1966 | 5329030 |
| the karimui trial of bcg. 2. tuberculin reactions in a leprosy-endemic but tuberculosis-free population. | | 1966 | 5330190 |
| isolation of a group-specific polysaccharide from tissues infected with mycobacterium leprae. | | 1966 | 5330864 |
| dapsone-resistant lepromatous leprosy in england. | | 1966 | 5331188 |
| sensitivity of mycobacterium leprae to low levels of 4,4'-diaminodiphenyl sulfone. | | 1966 | 5331452 |
| activity of repository sulfones against mycobacterium leprae in mice. | | 1967 | 5335647 |
| oxidation of 3,4-dihydroxyphenylalanine (dopa) by mycobacterium leprae. | | 1967 | 5336369 |
| relation of infection to tissue temperature in mice infected with mycobacterium marinum and mycobacterium leprae. | intravenous and footpad infections with mycobacterium marinum and footpad infections with m. leprae were compared in the following mouse strains: a/he, balb/c, cba, c3h, c57bl, c57l, dba, 101, and cfw. the results varied a great deal according to mouse strain used. intravenous injection of high doses of m. marinum resulted in deaths after 28 days of 100% of strain a/he, and none of strain 101; 27 days after injection, the feet and noses of all strain cba mice, but few of the c57bl, 101, or cfw m ... | 1967 | 5337835 |
| enhanced susceptibility of thymectomized and irradiated mice to infection with mycobacterium leprae. | | 1966 | 5338822 |
| studies on mycobacterium leprae in media enriched by mycobacterial extracts. | | 1967 | 5338959 |
| a logarithmic index of bacilli in biopsies. i. method. | | 1967 | 5338960 |
| a logarithmic index of bacilli in biopsies. 2. evaluation. | | 1967 | 5338961 |
| histochemical changes in the tissues of different types of leprous lesions. | | 1966 | 5341751 |
| succinic dehydrogenase, histidase and acid phosphatase activities of liver homogenates of rats infected with mycobacterium leprae murium. | | 1969 | 5824001 |
| nerve involvement. comparison of experimental infections by mycobacterium leprae and mycobacterium lepraemurium. | | 1965 | 5854573 |
| uptake of organic and inorganic radioactive substances and multiplication of mycobacterium leprae murium on alkaline galactomannan medium. | | 1966 | 5963328 |
| the significance of mycobacterium leprae in the nasal mucosa, with special reference to chinese leprosy patients. | | 1967 | 6045750 |
| effect of dds on established infections with mycobacterium leprae in mice. | | 1967 | 6067036 |
| [histopathological investigation of the central nervous system in leprosy. i. distribution of para-rosanilin positive materials in leprosy with special relation to tissue injury]. | | 1984 | 6085732 |
| ultrastructural and histophysiological studies on the blood-nerve barrier and perineurial barrier in leprosy neuropathy. | onset and nature of ultrastructural changes in endoneurial vasa nervorum during the pathogenesis of leprosy neuropathy and possibly associated alterations in the "blood-nerve barrier" were investigated, together with perineurial barrier functioning, in mice infected 20-28 months previously with mycobacterium leprae and in (ageing) non-infected mice. barriers were tested by i.v. administration of markers (trypan blue and ferritin) 1-4 days before killing the mice. twenty-eight months after infect ... | 1984 | 6095579 |
| study of the involvement of the sciatic nerve following inoculation with m. leprae and other mycobacteria in the mouse foot pad. | in order to determine whether mycobacterium leprae alone produce the typical damage in the sciatic nerves of foot pad inoculated mice as demonstrated earlier, a comparative study was undertaken using various other mycobacteria inoculated into the hind foot pads of normal swiss white mice. the findings indicate that fmr isolates no. 51 and no. 75 and m. avium showed multiplication in the foot pads of the mice throughout the 4th, 6th or 8th postinoculation months and these infections were associat ... | 1984 | 6100185 |
| do anti-leprosy drugs reach mycobacterium leprae in peripheral nerves? | | 1981 | 6110969 |
| occurrence of gamma-glutamyl transpeptidase activity in several mycobacteria including mycobacterium leprae. | gamma-glutamyl transpeptidase (gamma-gt) activity, which catalyzes the transfer of the "gamma-glutamyl" group of gamma-glutamyl compounds to several dipeptide and amino acid acceptors, was found to be present in several mycobacteria, including m. leprae, both in cell suspensions and in cell-free sonicates. glycyl d-amino acids were active as acceptors, particularly glycyl-d-alanine and alpha, epsilon-diaminopimelic acid, among the amino acids. two mycobacterial isolates obtained from biopsy mate ... | 1981 | 6114046 |
| glutamic acid decarboxylase in mycobacterium leprae. | suspensions of mycobacterium leprae purified from the organs (mostly spleen) of experimentally-infected armadillos (dasypus novemcinctus, linn) decarboxylated 1-(14c) glutamic acid liberating 14co2. the reaction was pyridoxal phosphate-dependent and was inhibited by hydroxylamine, suggesting that it is a true amino acid decarboxylase. loss of the activity at higher temperatures indicated the enzymatic nature of the reaction. excess substrate or substrate analogs inhibited the decarboxylase where ... | 1983 | 6137201 |
| activity of ansamycin against mycobacterium leprae in mice. | | 1983 | 6138619 |
| leprosy 1984. | | 1984 | 6143080 |
| metabolism in mycobacterium leprae: its relation to other research on m. leprae and to aspects of metabolism in other mycobacteria and intracellular parasites. | recently, some knowledge of metabolic pathways, rather than individual enzyme activities of m. leprae, is becoming available. ultimately this may be useful in devising culture media for m. leprae. knowledge restricted to individual reactions may be misleading. for instance, the detection of glcnacase and beta-glucuronidase and the subcellular localization of hyaluronic acid led to attempts to cultivate m. leprae on hyaluronic-acid based medium. subsequent investigations suggested that there was ... | 1984 | 6144638 |
| ansamycin activity against rifampicin-resistant mycobacterium leprae. | | 1984 | 6144865 |
| intermittent chemotherapy of experimental leprosy in mice. | in this study we assess the degree of prolonged bacteriostasis of mycobacterium leprae after temporary exposure to ehtionamide or thiacetazone, and relate this to their efficacy when administered intermittently to mice with experimental leprosy infections. the results show that temporary exposure of m. leprae to either of these drugs results in a prolonged bacteriostatic effect, but that efficacy is rapidly lost as the interval between doses is increased. using the mouse foot pad system, growth ... | 1980 | 6153254 |
| soluble blue as a counterstain in the ziehl-neelsen procedure--a brief communication. | a technique is described using soluble blue instead of the conventional methylene blue as a counterstain for the ziehl-neelsen procedure. this increases the intensity of blue staining of non-bacterial contaminants and is useful in monitoring the progress of purification of m. leprae from host tissue. | 1980 | 6154018 |
| variation of in vitro lymphocyte responses to m. leprae antigen in borderline tuberculoid leprosy patients. | | 1980 | 6154019 |
| fluorescent leprosy antibody absorption (fla-abs) test for detecting subclinical infection with mycobacterium leprae. | | 1980 | 6156918 |
| immunological aspects of leprosy: ten years' activity at the armauer hansen research institute and prospects for further work. | | 1980 | 6156919 |
| microscopic counts carried out on mycobacterium leprae and m. tuberculosis suspensions. a comparison of three staining procedures. | standard smears of heat-killed mycobacterium leprae and m. tuberculosis h37rv were counted microscopically following staining by the ziehl-neelsen, auramine, and silver-methenamine methods. the numbers of stained bacillary bodies were consistently higher in the silver-methenamine stained smears compared to the ziehl-neelsen and auramine stained smears. the auramine smears were examined under ultraviolet illumination and permitted the enumeration of the brightly fluorescent bacilli against a blac ... | 1980 | 6162812 |
| evaluation of fluorescent microscopy for detection of mycobacterium leprae. | | 1981 | 6166792 |
| [acid-alcohol resistance of m. leprae; importance of the staining technic in the microbiological diagnosis of leprosy]. | | 1980 | 6167135 |
| demonstration of antibodies reacting with different determinants on mycobacterium leprae antigen 7. | a purified preparation of m. leprae antigen 7 was used to investigate the specificity of anti-m. leprae 7 antibodies in leprosy sera in a radioimmunoassay. a solution containing arabinogalactan (ag) and arabinomannan (am) inhibited the antibodies in some sera to a great extent, whereas the antibody activity was virtually unchanged in other sera under the same conditions. these findings indicate that the antibodies are directed against different determinants on m. leprae antigen 7. antibodies aga ... | 1981 | 6168595 |
| the carbol fuchsin staining of mycobacteria in leprosy institutes of southeast asia and japan, especially the effect of "basic fuchsin" used in the carbol fuchsin formula. | | 1980 | 6170625 |
| antigen-specific suppressor cells in subclinical leprosy infection. | a two-stage in-vitro culture system was used to assay cells which suppress the lympho-proliferative response to mycobacterium leprae (ml). responses to ml, purified protein derivative of tuberculin, and streptokinase-streptodornase were preferentially suppressed by mitomycin-treated cells which had been primed with the same antigen in a 7-day primary culture. healthy subjects exposed to leprosy for more than 3 years showed strong suppression of the response to ml antigens (11 of 12 showed more t ... | 1981 | 6171698 |
| comparison of 22 species of mycobacteria by immunodiffusion against an absorbed reference leprosy serum. | lithium acetate antigenic extracts of 22 species of acetone-treated mycobacteria were tested by immunodiffusion precipitation for reactivity with a pool of sera from treated lepromatous leprosy patients (arls). this arls had been adsorbed with m. bovis (bcg, m. vaccae, cardiolipin, and lecithin to make it specific for m. leprae when used in an indirect immunofluorescence test. the arls produced two precipitin lines with m. leprae extract, one of which formed a line of identity with extracts of m ... | 1981 | 6172393 |
| studies on the defect in cell-mediated immunity in lepromatous leprosy using hla-d-identical siblings. absence of circulating suppressor cells and evidence that the defect is in the t-lymphocyte, rather than the monocyte, population. | sixteen healthy siblings were identified as hla-d-identical to 12 borderline lepromatous or polar lepromatous leprosy patients by the absence of a mixed lymphocyte reaction (mlr). the peripheral blood mononuclear cells (pbm) of the healthy siblings showed a lymphoproliferative response (delta cpm) to mycobacterium leprae antigens which was about fivefold or more greater than that of the lepromatous patients. lepromatous pbm, with or without mitomycin c treatment, were co-cultured with a constant ... | 1982 | 6176016 |
| production and partial characterization of monoclonal antibodies to mycobacterium leprae. | monoclonal antibodies to mycobacterium leprae were produced by the fusion of balb/c splenocytes and lymph node cells to balb/c myeloma (nsi/1) cells. eleven monoclonal antibodies were characterized as to their reactivity with m. leprae and 18 other mycobacterial species by enzyme-linked immunosorbent assay and immunofluorescence. two monoclonal antibodies reacted only with m. leprae, and the other nine showed unique patterns of reactivity by enzyme-linked immunosorbent assay. one monoclonal anti ... | 1982 | 6179876 |
| comparative ultrastructure of mycobacterium leprae and m. avium grown in experimental hosts. | | 1982 | 6180669 |
| taxonomy of mycobacterium leprae and m. lepraemurium. | | 1982 | 6180670 |
| a fluorescent staining procedure for determining the viability of mycobacterial cells. | a fluorescent staining procedure has been developed which rapidly, accurately, and economically measures the viability of mycobacterial cells. m. smegmatis and m. phlei have served as prototype organisms to establish conditions which ensure optimal staining. the staining method incorporates the use of the fatty acid ester fluorescein diacetate (fda) and ethidium bromide (eb). non-polar, non-fluorescent fda enters live cells where it is enzymatically hydrolyzed by acetylesterase to polar, fluores ... | 1982 | 6180992 |
| in vitro stimulation of lymphocytes in leprosy patients, healthy contacts of leprosy patients, and subjects not exposed to leprosy. comparison of an antigen fraction prepared from mycobacterium leprae and tuberculin-purified protein derivative. | in vitro lymphocyte stimulation was performed on peripheral blood lymphocytes from 48 leprosy patients, 15 healthy contacts of leprosy patients, and 16 normal controls who lived in a leprosy-free area and who had not been exposed to leprosy. tuberculin ppd and an antigen fraction. mlw 1, prepared from m. leprae, were used as stimulants. the mlw 1 preparation contained one antibody-precipitable component when tested in crossed immunoelectrophoresis against a polyvalent anti-m. leprae immunoglobul ... | 1982 | 6182610 |
| t-cell-dependent polyclonal activation by soluble mycobacterial extracts of b cells in peripheral blood mononuclear cell populations from leprosy patients and normal donors. | soluble extracts of ultrasonically disrupted mycobacteria (including mycobacterium leprae) cause strong t-cell-dependent, cyclosporin a-sensitive, polyclonal b-cell activation, in human peripheral blood mononuclear cell preparations. it is not enhanced by cortisol. cells from cases of lepromatous leprosy show no polyclonal b-cell activation in the presence of extracts of m. leprae, although they respond normally to other mycobacterial species. the polyclonal b-cell activation response to the m. ... | 1982 | 6183191 |
| sds-page analysis of m. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos. | studies have been conducted to characterize m. leprae bacilli derived from infected armadillos. first, the proteins of the mycobacterial extracts were fractionated by sds-page. subsequently, the proteins in the gel were electrophoretically transferred on a strip of nitrocellulose paper by the technique of 'electrophoretic blotting'. the separated bacterial protein bands, thus immobilized on the nitrocellulose paper were made to react immunologically with sera from the lepromatous patients, infec ... | 1982 | 6184189 |
| structure and antigenicity of the major specific glycolipid antigen of mycobacterium leprae. | earlier we reported on the presence of a specific phenolic glycolipid (phenolic glycolipid-i) in mycobacterium leprae, and in infected armadillo tissues (hunter, s. w., and brennan, p. j. (1981) j. bacteriol. 147, 728-735). it had an inherent oligosaccharide, composed of 3-o-me-rhamnose, 2,3-di-o-me-rhamnose, and 3,6-di-o-me-glucose, glycosidically linked to the phenol substituent. the structure of the oligosaccharide has now been determined, by partial acid hydrolysis, permethylation, 1h nmr, a ... | 1982 | 6184369 |
| lack of correlation between morphological index and viability as assessed by the uptake of 3h-thymidine by macrophage resident m. leprae. | thirty strains of m. leprae derived from skin biopsies of lepromatous leprosy patients were scored for morphological index (mi) and concurrently maintained for 2 weeks in macrophage cultures containing 3h thymidine. selective and significant incorporation of the radioactive label was observed in cultures containing freshly extracted m. leprae as compared to control cultures with autoclaved bacilli from the same biopsy. the percentage incorporation of 3h thymidine ranged from 103 to 1140%. morpho ... | 1982 | 6184544 |
| [variants of the del río hortega ammoniacal silver carbonate metallic impregnation technic with special application to i: melanin and amastigotes of trypanosoma cruzi; ii: basal membranes; iii: fungi and hansen's bacilli]. | | 1980 | 6184756 |
| effect of purified protein derivative and sonicates of mycobacterium leprae and mycobacterium bovis bcg on thromboplastin response in human monocytes in vitro. | human monocytes isolated from peripheral blood responded with increased thromboplastin expression upon stimulation in vitro with three mycobacterial antigens: tuberculin purified protein derivative and sonicates of mycobacterium bovis bcg and mycobacterium leprae. the stimulating principle of mycobacteria is probably a cell wall constituent since crude extracts of cell walls were 2.5 to 25 times more potent in stimulating thromboplastin synthesis than were whole sonicates. this thromboplastin re ... | 1982 | 6185426 |
| in vitro induction of human suppressor t cells by mycobacterial antigens. bcg activated okt4+ cells mediate suppression of antigen induced t cell proliferation. | peripheral blood mononuclear cells (pbmc), obtained from bcg vaccinated healthy donors, were induced to proliferation by bcg for five days in vitro. when re-exposed to bcg, they failed to proliferate. however, they partially retained the ability to respond to con a and allogeneic cells. the addition of graded numbers of such cultured cells to fresh autologous pbmc suppressed their proliferative response to bcg. these suppressor cells could also inhibit the proliferation of fresh cells to other m ... | 1983 | 6190596 |
| composite skin contact smears in multibacillary leprosy patients. | "composite skin contact smears" technique was used in 20 multibacillary leprosy patients to find out the presence of afb on their intact skin. one hundred and twenty one afb were found in 930 sq.cm. area of skin surface examined. seventy-five afb were solidly staining and 46 were granular. the bacilli were found in all the sites examined. immunofluorescent staining showed m. leprae in one out of four patients. the number of bacilli discharged from the intact skin seems adequate for the transmiss ... | 1982 | 6191122 |
| treatment of sulphone resistant leprosy--a review of sixty one cases. | | 1982 | 6191123 |
| comparison of the cytoplasmic antigens of leprosy-derived corynebacteria and some mycobacteria. | the immunological relationship between leprosy-derived corynebacteria (ldc) and reference mycobacteria was analysed by crossed immunoelectrophoresis with intermediate gel. for this purpose, three reference systems (ldc15/anti-ldc15, ldc18/anti-ldc8, and ldc8/anti-ldc8) were developed. they showed 15 to 20 distinct antigenic components in ldc cytoplasm. extensive cross-reactivity was observed among different ldc isolates, affecting 3 to 17 components. moreover, several components were shown to cr ... | 1983 | 6191381 |
| definition of species specific and cross-reactive antigenic determinants of mycobacterium leprae using monoclonal antibodies. | four soluble antigens of mycobacterium leprae have been identified using 12 murine monoclonal antibodies. their specificity, taxonomic distribution and molecular nature were analysed by radioimmunoassays and by immunoblotting from polyacrylamide electrophoresis gels. a protein antigen my1 (12k) reacted with one antibody (ml06) without demonstrable cross-reactivity for any of the other 20 tested species of mycobacteria. another four antibodies which identified antigen my2 revealed only a marginal ... | 1983 | 6191891 |
| serological specificity of phenolic glycolipid i from mycobacterium leprae and use in serodiagnosis of leprosy. | the serological activities of the specific phenolic glycolipid i from mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-m. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. high anti-phenolic glycolipid i immunoglobulin m antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tub ... | 1983 | 6193065 |
| bacteriology of mycobacterium leprae. | | 1983 | 6193386 |
| the degradation of mycobacterium leprae by a comparison of its staining properties. | the disintegration of mycobacterium leprae is revealed by a study of its acid-fast component, its non-acid-fast cell walls using methenamine silver, and its bcg-positive cytoplasmic component. solid bacilli stain by the three stain techniques used to identify these products, but the bcg component is demonstrated only with difficulty and appears granular. degradation of m. leprae is fairly rapid in bt, bb, and bl, and clearance of bacillary products occurs almost simultaneously because of the des ... | 1983 | 6194126 |
| characterization and functional studies of the murine t-lymphocyte response to mycobacterium leprae antigen. | mice were immunized with mycobacterium leprae in incomplete freund's adjuvant, and sensitized lymphocytes were obtained from draining lymph nodes. the lymphocytes thus obtained proliferated specifically in vitro in the presence of m. leprae antigen, and this response was shown to be both t-cell and macrophage dependent. t-cell blasts generated in vitro in response to m. leprae antigen were grown in the presence of interleukin-2 (il-2). the proliferative response of these blasts to m. leprae anti ... | 1983 | 6194557 |
| effect of pyridine extraction on the acid fastness of mycobacteria. | the effect of pyridine extraction on mycobacterial acid-fastness has been studied using m. leprae and some cultivable mycobacteria. the results revealed that not only m. leprae but some other mycobacteria, notably, m. vaccae and m. phlei also lose their acid-fastness when extracted with fresh pyridine for 2 hours at room temperature. the implication of these findings is discussed. | 1983 | 6195453 |
| the chemotherapy of leprosy. | | 1983 | 6195695 |
| growth of mycobacterium leprae in a redox system. | mycobacteria recovered from human lepromatous nodules and presumably m. leprae, have been grown in a medium that ensured a minimal oxygen tension at initiation of growth, and an increasing availability of oxygen as bacillary growth increased requiring marginal increments in oxygen tension. this physicochemical environment was achieved by the addition of strong biological reductants in the medium, and a combination of partial vacuum and alkaline pyrogallol in the culture vessel. in addition, n-te ... | 1983 | 6197589 |
| [stain behavior of mycobacterium leprae. a historical review]. | | 1983 | 6197746 |
| [modification of the basic stain of the ziehl-neelsen technic in the identification of mycobacterium leprae]. | authors analyse the counterstain with methylene blue solution in the usual ziehl-neelsen method. considerations are made on the characteristics of the dye substance. a technique alteration is proposed: a "concomitant alkalization" of the classic aqueous methylene blue solution, by adding some drops of sodium hydroxide solution, 1:500, on the slide at the moment of the staining. by this technique it was observed: a) a larger validity period of the solution; b) an absence of precipitate in the sli ... | 1982 | 6198275 |
| a serological test for leprosy based on competitive inhibition of monoclonal antibody binding to the my2a determinant of mycobacterium leprae. | a novel serological assay for leprosy has been devised on the basis of serum inhibition of binding of 125i-labelled ml04 monoclonal antibody to mycobacterium leprae sonicate-coated microtitre plates. antibodies were detected in 93% of lepromatous leprosy patients, whereas controls from the endemic area, including leprosy contacts and patients with tuberculosis, were serologically negative. the specificity and efficacy of the test may offer an advantage over previously used techniques. | 1983 | 6198787 |
| acid-fast bacilli on buffy coat smears in the acquired immunodeficiency syndrome: a lesson from hansen's bacillus. | a patient with the acquired immunodeficiency syndrome was found to have a continuous bacillemia of mycobacterium avium-intracellulare by examination of kinyoun-stained buffy coat smears. there were 29 cells/cu mm that contained acid-fast bacilli (afb) and 1.5 x 10(5) afb/ml of whole blood. the cells of the reticuloendothelial system were engorged with afb, suggesting reticuloendothelial saturation. the peripheral blood involvement and magnitude of the mycobacterial burden are analogous to lepros ... | 1984 | 6199851 |
| serodiagnosis of infection with mycobacterium leprae. | | 1983 | 6200450 |
| enumeration of purified suspensions of mycobacterium leprae. | previously described methods of counting noncultivable bacteria have a number of drawbacks including unpredictable variation due to differential staining, low reproducibility between replicate sample smears, and inexact estimations of the bacillary population due to the non-normal distribution of bacilli across the counting field. a simple method is described which minimizes the disadvantages of previous methods and allows the application of a stratified sample technique which improves the accur ... | 1984 | 6200451 |
| analysis of the major antigenic determinants of the characteristic phenolic glycolipid from mycobacterium leprae. | antibodies to the major phenolic glycolipid purified from mycobacterium leprae have been demonstrated previously in sera of leprosy but not tuberculosis patients using an elisa. the major antigenic determinants on this molecule were investigated using antisera raised in rabbits to the purified glycolipid and with a pool of sera from human lepromatous leprosy patients. a small, but significant cross-reaction was observed with the glycolipids from m. bovis and m. kansasii, which contain the phenol ... | 1984 | 6201310 |
| staining tissue-derived mycobacterium leprae with fluorescein diacetate and ethidium bromide. | a fluorescent staining procedure incorporating the use of fluorescein diacetate (fda) and ethidium bromide (eb) has previously been shown to accurately measure the viability of saprophytic mycobacterial cells. green-stained cells were shown to be viable and red-stained cells, dead. staining mycobacterium leprae cells with fda/eb, however, was complicated by interfering tissue components which masked the presence of stained bacteria. a petroleum ether separation technique enables m. leprae to be ... | 1984 | 6202650 |
| skin testing with mycobacterial reagents in leprosy. | | 1984 | 6203199 |
| use of an artificial antigen containing the 3,6-di-o-methyl-beta-d-glucopyranosyl epitope for the serodiagnosis of leprosy. | the coupling of synthetic 3,6-di-o-methyl-beta-d-glucopyranosyl-(1----4)-2,3-di-o-methyl-alpha-l -rhamnopyranose, the hapten determinant of phenolic glycolipid i from mycobacterium leprae, to bovine serum albumin (bsa) by reductive amination produced the antigen epsilon-n-1-[1-deoxy-2,3-di-o-methyl-4-o-(3',6'-di-o-methyl-beta -d-glucopyranosyl)-rhamnitol]-lysyl-bsa, which proved highly sensitive in elisa and showed good concordance with the native glycolipid in analysis of serum samples from 223 ... | 1984 | 6207246 |
| immunological unresponsiveness in leprosy. | the various forms of leprosy form a clinical and immunological spectrum which offers extraordinary possibilities for insight into immunoregulatory mechanisms in man. at one pole, tuberculoid leprosy, patients develop high levels of cell-mediated immunity which ultimately results in killing of bacilli in the tissues, albeit often with damage to nerves. at the lepromatous pole, patients exhibit selective immunological unresponsiveness to antigens of m. leprae. even though all currently known prote ... | 1984 | 6208143 |
| [staining methods used in the identification of mycobacterium leprae. historical review]. | | 1984 | 6209782 |
| defect in the generation of cytotoxic t cells in lepromatous leprosy. | cytotoxic t cells are consistently produced in normal individuals after in vitro stimulation by a pool of mitomycin-treated normal lymphocytes. patients suffering from lepromatous leprosy (ll), presenting with large amounts of mycobacterium leprae and without a history of erythema nodosum leprosum (enl) are unable to generate such cytotoxic t cells, while lepromatous patients with enl which, in the present study were all deprived of m. leprae, react normally. | 1982 | 6214342 |
| imbalances in t cell subpopulations in lepromatous leprosy. | circulating t cells, t helper, and t suppressor cells were investigated in 24 lepromatous patients, using murine hybridoma-derived monoclonal antibodies okt 3, okt 4, and okt 8. six bacillary lepromatous patients without recent enl were studied; in this group, suppressor cells were increased and helper cells diminished, resulting in a decrease in the helper/suppressor (h/s) ratio. nine bacteriologically negative lepromatous patients without recent enl were studied. t cell subsets distribution wa ... | 1982 | 6216219 |
| the cutaneous infiltrates of leprosy: cellular characteristics and the predominant t-cell phenotypes. | we report on the characteristics of cells in the cutaneous lesions and blood of 21 patients with lepromatous, tuberculoid, and intermediate forms of leprosy. a large proportion of the infiltrates in lepromatous lesions consist of macrophages heavily parasitized with mycobacterium leprae. the t cells in the lesions are devoid of okt4/leu 3a-positive ("helper") cells and consist almost exclusively of okt8/leu 2a-positive ("suppressor") populations. in contrast, the tuberculoid infiltrates contain ... | 1982 | 6216407 |
| the epidemiological evaluation, in burma, of the skin test reagent lra6; a cell-free extract from armadillo-derived mycobacterium leprae. part 1: leprosy patients. | | 1982 | 6220986 |
| the epidemiological evaluation, in burma, of the skin test reagent lra6; a cell-free extract from armadillo-derived mycobacterium leprae. part 2: close contacts and non-contacts of bacilliferous leprosy patients. | | 1982 | 6220987 |
| mechanism of immunosuppression in leprosy: presence of suppressor factor(s) from macrophages of lepromatous patients. | human peripheral blood mononuclear cell proliferation induced by mycobacterium leprae could be inhibited by the suppressor factor in the lysate of the macrophages of lepromatous leprosy patients. macrophages from normal subjects and tuberculoid patients did not show production of a suppressor factor. inhibition occurred only when the factor was present in the initial stages of lymphocyte culture. the factor is heat stable and nondialyzable. proliferation induced by some mycobacteria and concanav ... | 1983 | 6221997 |
| immunology of human leprosy--current status. | | 1983 | 6224992 |
| reactions in leprosy. | | 1983 | 6224993 |
| mechanisms of t-cell unresponsiveness in leprosy. | we analysed the mechanisms of t-cell unresponsiveness to mycobacterium leprae antigens and to unrelated antigens or t-cell mitogens in human leprosy and in an experimental model of murine infection by m. lepraemurium (mlm). in human leprosy, monoclonal antibodies okt3, okt4 and okt8 were used to enumerate t-cell subpopulations within peripheral blood. increased percentages of okt8+ cytotoxic/suppressor cells were observed in untreated, non-reactional lepromatous patients. conversely, lepromatous ... | 1983 | 6226239 |
| early events in the host-parasite relationship and immune response in clinical leprosy: its possible importance for leprosy control. | | 1983 | 6228348 |
| suppressor cell activity and phenotypes in the blood or tissues of patients with leprosy. | suppressor cell activity has been demonstrated in the peripheral blood of patients with leprosy. cells bearing the suppressor/cytotoxic phenotype have been enumerated in both peripheral blood and tissues, and microanatomical differences in tissue distribution have been observed. this first generation of studies has been characterized by considerable disagreement, a not unusual circumstance in the study of leprosy. in the case of blood suppressor cell activity, there appears to be no doubt as to ... | 1983 | 6228349 |
| immunological aspects of human leprosy. | | 1983 | 6230487 |
| the growth of mycobacterium leprae in nude mice. | | 1983 | 6230496 |
| exochelin-mediated iron uptake into mycobacterium leprae. | iron chelated to the exochelins from mycobacterium neoaurum was taken up by a suspension of m. leprae, prepared from the liver of an infected armadillo, over 15 hr. no uptake occurred when the iron was chelated with exochelins from m. bovis bcg or m. smegmatis or to a single exochelin from m. vaccae. uptake appeared to be by facilitated diffusion since it was not inhibited by either hgcl2, nan3, or 2,4-dinitrophenol. this was similar to the mode of uptake of ferriexochelin into m. neoaurum itsel ... | 1983 | 6231257 |
| investigations related to the development of a leprosy vaccine. | | 1983 | 6231259 |
| cultivation of mycobacterium x from mycobacterium leprae-infected tissues in propane-tetradecane-humic acid medium. | | 1984 | 6231260 |
| hla-d identity in a family with multiple cases of multibacillary leprosy. | | 1984 | 6231441 |
| [control of leprosy]. | | 1984 | 6238608 |
| [symposium on mechanisms of host defense from fundamental and clinical medicine. 5. genetic control of immune response in humans and its clinical significance]. | | 1984 | 6241938 |