| vibrionaceae as a possible source of qnr-like quinolone resistance determinants. | to gain insight into the functionality of qnr-like genes of several bacterial species of vibrionaceae that may encode quinolone resistance determinants. | 2005 | 16227349 |
| [faster detection of vibrio parahaemolyticus in foods by fq-pcr technique]. | to establish a rapid and accurate qualitative and quantitative method to detect vibrio parahaemolyticus in food. | 2005 | 16229276 |
| rapid detection of tdh and trh mrnas of vibrio parahaemolyticus by the transcription-reverse transcription concerted (trc) method. | we developed a novel method named the transcription-reverse transcription concerted (trc) method and an instrument that allowed rapid and completely homogeneous real-time monitoring of rna isothermal sequence amplification without any post-amplification analysis in our previous study [ishiguro et al., anal. biochem., 314, 77-86 (2003)]. in this study, we newly established rapid and sensitive trc systems for the detection of the mrnas transcribed from two major virulence genes of vibrio parahaemo ... | 2004 | 16233699 |
| [studies on extracellular virulent factors produced by potential pathogenic bacteria isolated from abalone postlarvae and biofilms]. | to find out the potential pathogen(s) that caused massive death of abalone postlarvae( haliotis diversicolor supertesta ) in southern china, 105 bacterial strains were isolated from the water, whitened postlarvae and their biofilms of an abalone farm in guangdong province. extra-cellular protease, gelatinase, lipase as well as haemolysis tests were performed on them. lysophospholipase (tlh)-targeted pcr was also carried out in order to reveal if the haemolysis caused by bacterial strains were re ... | 2005 | 16245888 |
| [quantitative detection of vibrio parahaemolyticus by real-time taqman pcr]. | vibrio parahaemolyticus is one of important human food pathogens. traditional diagnostic tests for v. parahaemolyticus are laborious and always present false negative results. therefore, it is important to develop a nucleic acid-based test for quantitative detection of v. parahaemolyticus. a taqman pcr assay was presented for quantitative detection of v. parahaemolyticus in pure cultures and oysters. the primers and probe were designed according to the gyrase b gene (gyrb) sequence of v. parahae ... | 2005 | 16245889 |
| characterization of vibrio parahaemolyticus isolated from coastal seawater in peninsular malaysia. | twenty-one vibrio parahaemolyticus isolates representing 21 samples of coastal seawater from three beaches in peninsular malaysia were found to be sensitive to streptomycin, norfloxacin and chloramphenicol. resistance was observed to penicillin (100%), ampicillin (95.2%), carbenicilin (95.2%), erythromycin (95.2%), bacitracin (71.4%), cephalothin (28.6%), moxalactam (28.6%), kanamycin (19.1%), tetracycline (14.3%), nalidixic acid (9.5%) and gentamicin (9.5%). plasmids of 2.6 to 35.8 mda were det ... | 2005 | 16295549 |
| purification of a novel arthropod defensin from the american oyster, crassostrea virginica. | an antimicrobial peptide was purified from acidified gill extract of a bivalve mollusk, the american oyster (crassostrea virginica), by preparative acid-urea--polyacrylamide gel electrophoresis and reversed-phase high performance liquid chromatography. the 4265.0 da peptide had 38 amino acids, including 6 cysteines. it showed strongest activity against gram-positive bacteria (lactococcus lactis subsp. lactis and staphylococcus aureus; minimum effective concentrations [mecs] 2.4 and 3.0 microg/ml ... | 2005 | 16297885 |
| use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media. | identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ... | 2005 | 16300063 |
| [growth inhibition of vibrio parahaemolyticus in seafood by tabletop dry ice cooler]. | tabletop dry ice coolers (three types; dome model, cap model and tripod model), which are used in kitchens and hotel banquet halls to refrigerate fresh seafood, were investigated to determine whether growth of vibrio parahaemolyticus was inhibited by their use. on tsa plates containing 1.8% nacl and fresh seafood (fillets of squid, pink shrimp and yellowtail), v. parahaemolyticus (o3:k6, tdh+) inoculated at 4 to 5 log cfu/sample and left at ambient temperature (25 degrees c) grew by 1.0 to 2.8 o ... | 2005 | 16305176 |
| [vibrio parahaemolyticus infections and algal intoxications as emergent public health problems in chile]. | there is interest in the paradigm that relates environmental sea changes to the emergence of diseases that affect both aquatic organisms in the sea and human beings. the emergence of vibrio parahaemolyticus as an important cause of epidemic summer diarrhea in 2004 and 2005, confined mainly to the tenth region in chile, could be a manifestation of this trend. this and other areas of the country have also experienced several outbreaks of paralytic shellfish poisoning (psp), diarrheal shellfish poi ... | 2005 | 16311702 |
| oyster-associated outbreaks of norovirus gastroenteritis in singapore. | outbreaks of gastroenteritis associated with the consumption of raw imported half-shelled frozen oysters occurred in singapore between 16 dec 2003 and 04 jan 2004. a total of 305 cases were reported with clinical symptoms of diarrhoea (94%), abdominal cramps (72%), vomiting (69%) and fever (54%). the median incubation period was 30.8h and the duration of illness was 2-3 days. the overall relative risk of oyster consumption was 14.1 (95% ci: 8.3-24.0, p<0.001). stool and oyster samples tested neg ... | 2004 | 16321654 |
| sanitation of seawater effluent from seaweed processing plants using a photo-catalytic tio2 oxidation. | a fine porcelain open-cell photo-catalytic filter with titanium dioxide (tio(2)) was evaluated for sterilization and sanitation of bio-polluted industrial water. in simulated seawater industrial effluent samples, the populations of escherichia coli and vibrio parahaemolyticus quickly decreased and reached non-detectable level within 10min. in seawater effluents from a seaweed processing plant, the bacterial populations in two samples quickly decreased by more than 10(3). in another two samples t ... | 2006 | 16325650 |
| i've sold my sport-utility vehicle. | | 2005 | 16327816 |
| separation of active and inactive fractions from starved culture of vibrio parahaemolyticus by density dependent cell sorting. | the co-existence of physiologically different cells in bacterial cultures is a general phenomenon. we have examined the applicability of the density dependent cell sorting (ddcs) method to separate subpopulations from a long-term starvation culture of vibrio parahaemolyticus. the cells were subjected to percoll density gradient and separated into 12 fractions of different buoyant densities, followed by measuring the cell numbers, culturability, respiratory activity and leucine incorporation acti ... | 2005 | 16329866 |
| isolation and molecular characterization of toxigenic vibrio parahaemolyticus from the kii channel, japan. | studies were conducted on the ecology of potentially pathogenic vibrio parahaemolyticus in three coastal areas of kii channel, tokushima, japan. seawater and seaweed samples were collected seasonally between june 2003 and may 2004. total and toxigenic strains of v. parahaemolyticus were isolated using most probable number culture and colony blot hybridization. toxigenic strains were serotyped and further characterized by random amplified polymorphic dna (rapd) and ribotyping. six thousand strain ... | 2006 | 16338587 |
| partial purification and characterization of hemolysin from a psychrotrophic kanagawa-positive marine vibrio. | psychrotrophic kanagawa-positive marine vibrios were isolated from soft-shelled clams (mya arenaria) collected in yaquina bay, oreg. the 235 vibrio isolates obtained were screened for gram reaction and morphology, kanagawa reaction on wagastsuma agar, and response to selected biochemical tests. the vibrio selected for further study was grown in broth, and the hemolysin was precipitated from a cleared supernatant with solid ammonium sulfate. the hemolytic substance was partially purified by deae- ... | 1982 | 16345927 |
| efficiencies of recovery of bdellovibrios from brackish- water environments by using various bacterial species as prey. | a total of 44 bacterial species subdivided into 10 trial experiments have been used as prey for the recovery of bdellovibrios from samples of water from a brackish tidal pond and an aquarium saltwater tank. in an initial investigation, the recovery efficiency of each of the test bacterial species was compared with that of a designated standard prey, vibrio parahaemolyticus p-5. the results revealed that in each case strain p-5 yielded an equal or significantly greater number of plaques of bdello ... | 1990 | 16348096 |
| serogrouping of halophilic bdellovibrios from chesapeake bay and environs by immunodiffusion and immunoelectrophoresis. | little has been reported on the serological relationship of halophilic bdellovibrios (bd). immunodiffusion analysis performed with rabbit or mouse bd antisera developed against eight halophilic bd isolates and one terrestrial bd isolate, when reacted with soluble antigen preparations of 45 isolates of halophilic bd, allowed separation into seven serogroups, which were distinct from the terrestrial isolate. soluble antigen preparations of prey bacteria, vibrio parahaemolyticus p-5 (p-5) and esche ... | 1991 | 16348597 |
| [symptoms of food-borne diseases and gastroenteritis in kyushu, japan]. | in this study we analyzed the symptoms of gastroenteritis or food-borne disease caused by the 10 most prevalent pathogens: norovirus, salmonella, vibrio parahaemolyticus, campylobacter jejuni, clostridium perfringens, shiga toxin-producing escherichia coli (stec), enterotoxigenic e. coli (etec), shigella sonnei/flexneri (shigella), staphylococcus aureus, and emetic-type bacillus cereus. the symptoms diarrhea, vomiting, fever, abdominal pain, and headache, and the incubation period in 646 cases i ... | 2005 | 16366357 |
| isolation of vibrio parahaemolyticus and vibrio vulnificus from wild aquatic birds in japan. | vibrio parahaemolyticus and vibrio vulnificus were isolated from faecal samples of wild aquatic birds in winter. although v. parahaemolyticus and v. vulnificus were present in low numbers in seawater in the area where the faecal samples of the birds were collected, the pathogens were isolated from the faeces of the birds. this study demonstrates that wild aquatic birds are a vehicle for v. parahaemolyticus and v. vulnificus to survive in winter. | 2006 | 16371182 |
| cloning, functional expression and primary characterization of vibrio parahaemolyticus k+/h+ antiporter genes in escherichia coli. | the regulation of internal na(+) and k(+) concentrations is important for bacterial cells, which, in the absence of na(+) extrusion systems, cannot grow in the presence of high external na(+). likewise, bacteria require k(+) uptake systems when the external k(+) concentration becomes too low to support growth. at present, we have little knowledge of k(+) toxicity and bacterial outward-directed k(+) transport systems. we report here that high external concentrations of k(+) at alkaline ph are tox ... | 2006 | 16390457 |
| [acquisition and evolution of virulence gene systems in pathogenic bacteria]. | | 2005 | 16411452 |
| sensitivities of foodborne pathogens to pressure changes. | eight foodborne pathogens were suspended in ultrahigh-temperature whole milk and treated at pressure levels of 0.1 to 690 mpa at 21.5 degrees c for 10 min. there was no clear trend in pressure resistance between gram-negative and gram-positive organisms. the order of the single strains tested, from most to least pressure sensitive, was vibrio parahaemolyticus < yersinia enterocolitica < listeria monocytogenes < salmonella enterica serovar typhimurium < s. enterica serovar enteritidis < escherich ... | 2006 | 16416910 |
| genomic comparisons among gamma-proteobacteria. | predicted highly expressed (phx) genes are compared for 16 gamma-proteobacteria and their similarities and differences are interpreted with respect to known or predicted physiological characteristics of the organisms. predicted highly expressed genes often reflect the organism's predominant lifestyle, habitat, nutrition sources and metabolic propensities. this technique allows to predict principal metabolic activities of the microorganisms operating in their natural habitats. among our findings ... | 2006 | 16423015 |
| analysis of the lateral flagellar gene system of aeromonas hydrophila ah-3. | mesophilic aeromonas strains express a polar flagellum in all culture conditions, and certain strains produce lateral flagella on semisolid media or on surfaces. although aeromonas lateral flagella have been described as a colonization factor, little is known about their organization and expression. here we characterized the complete lateral flagellar gene cluster of aeromonas hydrophila ah-3 containing 38 genes, 9 of which (lafa-u) have been reported previously. among the flgll and lafa structu ... | 2006 | 16428388 |
| identification of proteins secreted via vibrio parahaemolyticus type iii secretion system 1. | vibrio parahaemolyticus, a gram-negative marine bacterium, is an important pathogen causing food-borne gastroenteritis or septicemia. recent genome sequencing of the rimd2210633 strain (a kanagawa phenomenon-positive clinical isolate of serotype o3:k6) revealed that the strain has two sets of gene clusters that encode the type iii secretion system (ttss) apparatus. the first cluster, ttss1, is located on the large chromosome, and the second, ttss2, is on the small chromosome. previously, we repo ... | 2006 | 16428750 |
| differences in the api 20e biochemical patterns of clinical and environmental vibrio parahaemolyticus isolates. | genetic differences in clinical and environmental strains of vibrio parahaemolyticus have been widely used as criteria in identifying pathogenic isolates. however, few studies have been carried out to assess the differences in biochemical characteristics of v. parahaemolyticus isolates from human and environmental sources. we compared the biochemical profiles obtained by the characterization of v. parahaemolyticus isolates from human infections and the marine environment using the api 20e system ... | 2006 | 16436064 |
| detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays. | to detect the common intestinal pathogenic bacteria quickly and accurately. | 2005 | 16437687 |
| antibacterial activity of plant extracts from azuki beans (vigna angularis) in vitro. | this study was undertaken to examine the antimicrobial property of azuki beans (vigna angularis). the water extracts of green, black and red colored azuki beans showed antibacterial effects against staphylococcus aureus, aeromonas hydrophila and vibrio parahaemolyticus. in contrast, the extract of white azuki beans showed no inhibition towards any of the microorganisms examined. the extracts of colored azuki beans contained larger amounts of polyphenols including proanthocyanidins than the extra ... | 2006 | 16444673 |
| a reinvestigation of the secondary structure of functionally active vsglt, the vibrio sodium/galactose cotransporter. | the bacterial na(+)/galactose cotransporter vsglt of vibrio parahaemolyticus is a member of the sodium:solute symporter family (sss). previous studies using electron microscopy have shown that vsglt is a monomeric protein. computational and experimental topological analyses have consistently indicated that this protein possesses 14 transmembrane alpha-helices. our previous study using attenuated total reflectance fourier transform infrared spectroscopy (atr-ftir) to quantitate secondary structur ... | 2006 | 16445289 |
| antiviral activities of purified compounds from youngia japonica (l.) dc (asteraceae, compositae). | the ethanol extract of a biannual medicinal herb, youngia japonica (commonly known as oriental hawk's beard) was reported previously to have potent antiviral activity against respiratory syncytial virus (rsv) cultured in hep-2 cells. three anti-microbial agents, namely 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and luteolin-7-o-glucoside were subsequently purified and chemically characterized from the ethanol extract of youngia japonica. the two dicaffeoylquinic acids exhibited promin ... | 2006 | 16469463 |
| occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in spain. | to survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. | 2006 | 16478492 |
| application of real-time pcr for quantitative detection of vibrio parahaemolyticus from seafood in eastern china. | vibrio parahaemolyticus is recognized as a leading human food-borne pathogen. a taqman pcr assay based on the gyrase b gene (gyrb) sequence of v. parahaemolyticus was developed for quantitative detection of v. parahaemolyticus in seafood. the study involving 27 v. parahaemolyticus and 10 strains of other species indicated that the real-time pcr test was highly specific. the sensitivity of the assay was approximately a single cfu per pcr in pure culture and six to eight cfu per pcr in spiked raw ... | 2006 | 16487299 |
| variation in the 16s-23s rrna intergenic spacer regions in vibrio parahaemolyticus strains are due to indels nearby their trnaglu. | vibrio parahaemolyticus contains 11 rrna operons each including one of six 16s-23s rrna gene intergenic spacer classes differing in size and nucleotide sequence. some of the spacer classes may differ between isolates. we observed that the differences in the spacers between isolates are generally in two spacer classes present in single copies in the genome, one class containing trna(ala) and trna(glu) and the other trna(glu) exclusively. moreover, these differences are due to indels located nearb ... | 2006 | 16487317 |
| immunostimulation of white shrimp (litopenaeus vannamei) following dietary administration of ergosan. | ergosan an algal product containing 1% alginic acid, developed for use in aquaculture and reported to have immunomodulatory activity, was administered orally to intermoult adult white shrimp (litopenaeus vannamei) for 15 days. examination of haemolymph proteins using sds-page did not reveal any obvious differences between control and ergosan treated shrimp. similarly, total haemocyte counts were found to be roughly equivalent for both the control and experimental samples. however, differential a ... | 2006 | 16490202 |
| [indicative vibrio parahaemolyticus strains for the detection and primary indentification of parahemolytic phages]. | | 2006 | 16512482 |
| cyclo(phe-pro) modulates the expression of ompu in vibrio spp. | vibrio vulnificus was found to produce a chemical that induced the expression of vibrio fischeri lux genes. electron spray ionization-mass spectrometry and 1h nuclear magnetic resonance analyses indicated that the compound was cyclo(l-phe-l-pro) (cfp). the compound was produced at a maximal level when cell cultures reached the onset of stationary phase. sodium dodecyl sulfate-polyacrylamide gel analysis of the total proteins of v. vulnificus indicated that expression of ompu was enhanced by exog ... | 2006 | 16513751 |
| detection of vibrio parahaemolyticus in shellfish by use of multiplexed real-time pcr with taqman fluorescent probes. | we developed a multiplexed real-time pcr assay using four sets of gene-specific oligonucleotide primers and four taqman probes labeled with four different fluorophores in a single reaction for detection of total and pathogenic vibrio parahaemolyticus, including the pandemic o3:k6 serotype in oysters. v. parahaemolyticus has been associated with outbreaks of food-borne gastroenteritis caused by the consumption of raw or undercooked seafood and therefore is a concern to the seafood industry and co ... | 2006 | 16517652 |
| cholera epidemic in baghdad during 1999: clinical and bacteriological profile of hospitalized cases. | an epidemic of cholera in iraq was anticipated for the year 1999 and a plan of notification and treatment of cases of diarrhoea was made. this paper documents the clinical and bacteriological profile of cholera cases admitted to the 6 hospitals in baghdad during the epidemic. the number of stool culture-positive cases was 874. the peak incidence of cases (june 1999) was earlier than previous epidemics with no cases registered in november/december 1999. all age groups were affected and the case f ... | 2005 | 16532666 |
| inactivation of vibrio parahaemolyticus and vibrio vulnificus in phosphate-buffered saline and in inoculated whole oysters by high-pressure processing. | inactivation studies for vibrio parahaemolyticus tx-2103 (serotype o3:k6) and vibrio vulnificus mo-624 (clinical isolate) were conducted in phosphate-buffered saline (pbs) and in inoculated oysters under high-pressure processing conditions. v. parahaemolyticus was more resistant than v. vulnificus in pbs at all pressures and times. a 6-log reduction of v. parahaemolyticus and v. vulnificus in pbs at 241 mpa required 11 and 5 min, respectively, which included a 3-min pressure come-up time. a 4.5- ... | 2006 | 16541691 |
| three new regulators of swarming in vibrio parahaemolyticus. | movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in vibrio parahaemolyticus. expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. however, not all v. parahaemolyticus isolates swarm proficiently. the organism undergoes phase variation between opaque (op) and translucent (tr) cell types. the op cell produces copious capsular polysaccharide and swarms poorly, whereas the ... | 2006 | 16547050 |
| comparative hemolytic activity of vibrio parahaemolyticus and related vibrios. | the hemolytic activities of 91 strains of vibrio parahaemolyticus isolated from human diarrheal stools, sea fish, and sea water; 21 suspected v. parahaemolyticus cultures isolated from wound infections; 14 nonpathogenic marine vibrios; and 21 v. parahaemolyticus isolated from moribund blue crabs callinectes sapidus were compared. potentially pathogenic v. parahaemolyticus strains could be differentiated from the related nonpathogenic marine vibrios, because the former hemolyzed hamster, sheep, a ... | 1970 | 16557747 |
| vibrio parahaemolyticus in shellfish and clinical samples during two large epidemics of diarrhoea in southern chile. | large epidemics of diarrhoea associated with seafood consumption and vibrio parahaemolyticus occurred during the austral summers of 2004 and 2005 in the environs of puerto montt, chile (41 degrees 29's 72 degrees 24'w). there are no reports of v. parahaemolyticus infections before 2004 in this region, their absence being explained by the low ocean temperatures which seldom reach 16 degrees c. we analysed v. parahaemolyticus obtained from shellfish and clinical samples during epidemics. isolates ... | 2006 | 16584479 |
| occurrence of vibrio parahaemolyticus, v. cholerae, and v. vulnificus in norwegian blue mussels (mytilus edulis). | vibrio parahaemolyticus, v. cholerae, and v. vulnificus were isolated from 10.3%, 1.0%, and 0.1% of 885 blue mussel samples, respectively. four of the samples contained trh(+) v. parahaemolyticus, while no tdh-positive isolates were detected. the v. cholerae isolates were non-o:1/non-o:139 serotypes and were ctxa negative. | 2006 | 16598019 |
| biochemical fingerprinting of vibrio parahaemolyticus by the pheneplate system: comparison between pandemic and non-pandemic serotypes. | during recent years a pandemic clone of vibrio parahaemolyticus has emerged. isolates of this clone are distributed among several serotypes, but are genotypically related. in the present study, a phenotyping method (biochemical fingerprinting) was used to characterize pandemic and non-pandemic isolates belonging to v. parahaemolyticus. it was found that the pandemic isolates showed a high level of phenotypic homogeneity and a majority of the pandemic isolates belonged to the same biochemical phe ... | 2006 | 16650334 |
| four genomic islands that mark post-1995 pandemic vibrio parahaemolyticus isolates. | vibrio parahaemolyticus is an aquatic, halophilic, gram-negative bacterium, first discovered in 1950 in japan during a food-poisoning outbreak. infections resulting from consumption of v. parahaemolyticus have increased globally in the last 10 years leading to the bacterium's classification as a newly emerging pathogen. in 1996 the first appearance of a pandemic v. parahaemolyticus clone occurred, a new o3:k6 serotype strain that has now been identified worldwide as a major cause of seafood-born ... | 2006 | 16672049 |
| development and evaluation of a rapid, simple, and sensitive immunochromatographic assay to detect thermostable direct hemolysin produced by vibrio parahaemolyticus in enrichment cultures of stool specimens. | thermostable direct hemolysin (tdh) is considered to be a major virulence factor in vibrio parahaemolyticus, and most cases of v. parahaemolyticus diarrhea in humans are caused by tdh gene-positive strains. in the present study, we developed an immunochromatographic assay to detect tdh (tdh-ica) and evaluated the utility of tdh-ica for the diagnosis of v. parahaemolyticus diarrhea. tdh-ica allowed the detection of 0.2 ng/ml of tdh within 10 min. fecal homogenates were spiked with various numbers ... | 2006 | 16672412 |
| molecular characterization of thermostable direct haemolysin-related haemolysin (trh)-positive vibrio parahaemolyticus from oysters in mangalore, india. | pathogenic vibrio parahaemolyticus strains producing either or both of a thermostable direct haemolysin (tdh) and a tdh-related haemolysin (trh) encoded by tdh and trh genes, respectively, are isolated at a low rate from the environment. however, recently we observed that a considerable percentage of apw (alkaline peptone water) enrichment broths of oysters collected off mangalore india, were trh(+), rather than tdh(+) by pcr. in order to further investigate the prevalence and genetic diversity ... | 2006 | 16689720 |
| antimicrobial activities of cinnamon oil and cinnamaldehyde from the chinese medicinal herb cinnamomum cassia blume. | both cinnamomum verum j.s. presl. and cinnamomum cassia blume are collectively called cortex cinnamonmi for their medicinal cinnamon bark. cinnamomum verum is more popular elsewhere in the world, whereas c. cassia is a well known traditional chinese medicine. an analysis of hydro-distilled chinese cinnamon oil and pure cinnamaldehyde by gas chromatography/mass spectrometry revealed that cinnamaldehyde is the major component comprising 85% in the essential oil and the purity of cinnamaldehyde in ... | 2006 | 16710900 |
| vibrio parahaemolyticus growth under low-iron conditions and survival under high-magnesium conditions. | since 1996, vibrio parahaemolyticus serotype o3:k6 and closely related strains have been associated with an increased incidence of v. parahaemolyticus gastroenteritis worldwide, suggesting the emergence of strains with enhanced abilities to cause disease. one hypothesis for the recent emergence of v. parahaemolyticus o3:k6 and related strains is an enhanced capacity for environmental survival relative to other strains, which might result in increased human exposure to these organisms. therefore, ... | 2006 | 16715802 |
| levels of vibrio parahaemolyticus and thermostable direct hemolysin gene-positive organisms in retail seafood determined by the most probable number-polymerase chain reaction (mpn-pcr) method. | the incidence and levels of vibrio parahaemolyticus and thermostable direct hemolysin gene (tdh)-positive organisms in retail seafood were determined. the most probable number-polymerase chain reaction (mpn-pcr) method using a pcr procedure targeting the species-specific thermolabile hemolysin gene (tlh) and tdh was used to determine the levels of v. parahaemolyticus and tdh-positive organisms, respectively. in seafood for raw consumption, v. parahaemolyticus was found in four (13.3%) of 30 fish ... | 2006 | 16729663 |
| serum igm levels against select marine bacteria in the atlantic sharpnose shark (rhizoprionodon terraenovae) from three estuaries. | the atlantic sharpnose shark (rhizoprionodon terraenovae) is abundant and easily captured throughout the southeastern united states. therefore this species serves as an ideal model for generating basic immunological reagents to establish baseline information regarding the immunophysiology of sharks in the wild, and for attempting to correlate shark immune responses to potential pathogens with the quality of the habitat in which they reside. sharpnose shark serum igm was purified over a protein-a ... | 2006 | 16750427 |
| identification of a dna methyltransferase gene carried on a pathogenicity island-like element (vpai) in vibrio parahaemolyticus and its prevalence among clinical and environmental isolates. | in this study we identified a putative virulence-associated dna methyltransferase (mtase) gene carried on a novel 22.79-kb pathogenicity island-like element (vpai) in v. parahaemolyticus. the v. parahaemolyticus mtase gene was shown by pcr to be prevalent (>98%) in pandemic thermostable direct hemolysin gene-positive isolates, which suggests that vpai may confer unique virulence traits to pandemic strains of v. parahaemolyticus. | 2006 | 16751568 |
| [foodborne diseases occurred in 2003--report of the national foodborne diseases surveillance system, china]. | to study the foodborne disease outbreaks in the areas covered by national foodborne disease surveillance system. | 2006 | 16758972 |
| antimicrobial activity of marine bacteria associated with sponges from the waters off the coast of south east india. | seventy-five marine bacterial strains associated with four species of sponges (echinodictyum sp., spongia sp., sigmadocia fibulatus and mycale mannarensis) were isolated from the tuticorin coast, gulf of mannar region. the agar-overlay method was used to screen for antibiotic production by these strains against four bacteria, viz., bacillus subtilis, escherichia coli, vibrio parahaemolyticus, and vibrio harveyi and one fungal pathogen, viz., candida albicans. twenty-one per cent of the bacterial ... | 2006 | 16765842 |
| role of the rpos gene in the survival of vibrio parahaemolyticus in artificial seawater and fish homogenate. | vibrio parahaemolyticus is a foodborne pathogen isolated from coastal waters of the united states and from a variety of seafood, including fish. seawater represents a nutrient-limiting environment for v. parahaemolyticus. during its persistence in seawater, v. parahaemolyticus is exposed to a variety of environmental stresses, including hyperosmolarity, fluctuations in temperature, and cold stress. the alternate sigma factor of rna polymerase, designated as (rpos), encoded by the gene rpos has b ... | 2006 | 16786870 |
| the first outbreak of acute diarrhea due to a pandemic strain of vibrio parahaemolyticus o3:k6 in kolkata, india. | | 2007 | 16793305 |
| antimicrobial effect of spices and herbs on vibrio parahaemolyticus. | the antimicrobial effects of spices and herbs from 18 plant species were examined on a foodborne pathogen, vibrio parahaemolyticus, with the use of combinations of temperatures and nutrient levels. basil, clove, garlic, horseradish, marjoram, oregano, rosemary, and thyme exhibited antibacterial activities at incubation of 30 degrees c, while with the exception of horseradish, the same spices and additional 7 species exhibited the activities at 5 degrees c. the lowest mic (minimum inhibitory conc ... | 2006 | 16797760 |
| new cluster of plasmid-located class 1 integrons in vibrio cholerae o1 and a dfra15 cassette-containing integron in vibrio parahaemolyticus isolated in angola. | the resistance profile and its correlation with mobile genetic elements were investigated in 11 vibrio cholerae o1 and 2 vibrio parahaemolyticus clinical isolates, as well as in 1 v. cholerae o1 and 1 v. cholerae non-o1 environmental isolate, isolated between 1991 and 1996 in different provinces of angola. all clinical isolates of v. cholerae o1 were resistant to ampicillin, chloramphenicol, trimethoprim, sulfamethoxazole, and tetracycline. they also contained a large conjugative plasmid (p3iang ... | 2006 | 16801431 |
| antibacterial activity of citrus fruit juices against vibrio species. | lemon, lime and sudachi juices were tested for antibacterial activity against seven strains of vibrio species. all juices were effective in inhibiting the growth of the vibrio strains. citric acid, the major organic acid in these juices, was found to be responsible for inhibiting the growth of vibrio parahaemolyticus. sauce prepared from sudachi juice showed a strong bactericidal activity against vibrio parahaemolyticus, whereas the sauce adjusted to higher ph values had no bacterial activity. d ... | 2006 | 16802698 |
| genetic richness of vibriophages isolated in a coastal environment. | the purpose of this study was to characterize vibrio parahaemolyticus viruses (vpvs) isolated from different environments within and adjacent to the strait of georgia, and to examine the relative influences of distance and environment on host-range and genetic richness. nearly all seawater enrichment cultures (29/31) generated isolates, implying that vpvs were widespread in the virioplankton, yet at low abundances (< 1 l(-1)). viruses were not detected in sediments (n = 99). fourteen of the 16 v ... | 2006 | 16817925 |
| detection of free-living and plankton-bound vibrios in coastal waters of the adriatic sea (italy) and study of their pathogenicity-associated properties. | culturable vibrios were isolated from water and plankton fractions collected during an 18-month sampling study performed along the north-central coast of the adriatic sea (italy). unculturable vibrio vulnificus and v. parahaemolyticus were detected in plankton fractions by polymerase chain reaction amplification of dna sequences for cytotoxin-haemolysin and thermolabile haemolysin respectively. the presence of v. parahaemolyticus, v. vulnificus and v. cholerae virulence genes and the expression ... | 2006 | 16817938 |
| soft-agar-coated filter method for early detection of viable and thermostable direct hemolysin (tdh)- or tdh-related hemolysin-producing vibrio parahaemolyticus in seafood. | a novel method for detecting viable and thermostable direct hemolysin (tdh)-producing or tdh-related hemolysin (trh)-producing vibrio parahaemolyticus in seafood was developed. the method involved (i) enrichment culture, selective for viable, motile cells penetrating a soft-agar-coated filter paper, and (ii) a multiplex pcr assay targeting both the tdh gene (tdh) and trh gene (trh) following dnase pretreatment on the test culture to eradicate any incidental dnas that might have been released fro ... | 2006 | 16820446 |
| inhibition of vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (blis) produced by vibrio mediterranei 1. | the aim of this research was to identify and partially purify new bacteriocin-like substances from strains of halophilic 'non-cholera' vibrios isolated from food sources. | 2006 | 16834611 |
| pyrolysis mass spectrometry for distinguishing potential hoax materials from bioterror agents. | pyrolysis mass spectrometry (pyms) was investigated as a rapid tool to distinguish potential bioterror hoax materials from samples containing pathogenic bacteria. a pyrolysis time-of-flight (tof) mass spectrometer equipped with an alternative ionization technique, metastable atom bombardment (mab), was used to produce sample spectra. these spectra were analyzed by principal component and discriminant analysis for pattern recognition. materials investigated were two strains of vibrio parahaemolyt ... | 2006 | 16841357 |
| prevalence and effectiveness of an education program on intestinal pathogens in food handlers. | to assess the prevalence of intestinal pathogens and the effectiveness of an education program in food handlers in a tertiary care hospital. | 2005 | 16850637 |
| influence of vibrio parahaemolyticus mtcc 451 on the levels of ascorbic acid and histamine in penaeus monodon (fabricius). | juvenile shrimps (penaeus monodon) weighing about 15-20 gms were procured from the grow-out ponds and reared under laboratory conditions. they were challenged with vibrio parahaemolyticus mtcc 451 and their ld50 value was evaluated. the ascorbic acid and the histamine activity of both the control and experimental groups were assessed. the results showed a marked decline in both the parameters at all intervals. this reveals the pathogenesis of the inoculated bacterial strain, which affects the de ... | 2006 | 16850878 |
| [molecular typing on vibrio parahaemolyticus isolates from hangzhou, china, during 2000-2002]. | to study the molecular epidemiology of vibrio parahaemolyticus isolates from clinical and environmental samples collected in hangzhou area during 2000 and 2002. | 2006 | 16875541 |
| vibrio vulnificus and v. parahaemolyticus necrotising fasciitis in fishermen visiting an estuarine tropical northern australian location. | three cases of severe necrotising fasciitis due to vibrio vulnificus (two cases) and vibrio parahaemolyticus (one case, fatal), have occurred in caucasian tourists while fishing at a remote tropical northern australian estuarine area. infections were acquired over a 4-year period during the tourist fishing season (april to july 2000-2003), when water temperatures range from 23 to 30 degrees c. they are notable for their geographical clustering in the remote western aspect of the gulf of carpenta ... | 2007 | 16890991 |
| salmonella spp., vibrio spp., clostridium perfringens, and plesiomonas shigelloides in marine and freshwater invertebrates from coastal california ecosystems. | the coastal ecosystems of california are highly utilized by humans and animals, but the ecology of fecal bacteria at the land-sea interface is not well understood. this study evaluated the distribution of potentially pathogenic bacteria in invertebrates from linked marine, estuarine, and freshwater ecosystems in central california. a variety of filter-feeding clams, mussels, worms, and crab tissues were selectively cultured for salmonella spp., campylobacter spp., escherichia coli-o157, clostrid ... | 2006 | 16897302 |
| vibrio parahaemolyticus infections associated with consumption of raw shellfish--three states, 2006. | during may 20-july 31, 2006, new york city, new york state, oregon, and washington health departments reported a total of 177 cases of vibrio parahaemolyticus infection, of which 122 have been associated with 17 clusters. a cluster has been defined as a group of two or more ill persons who were linked to the same shellfish source (e.g., shared a meal at the same restaurant or obtained shellfish from the same seafood market). certain clusters were associated with restaurants, certain clusters wit ... | 2006 | 16902397 |
| structure of the class iv adenylyl cyclase reveals a novel fold. | the crystal structure of the class iv adenylyl cyclase (ac) from yersinia pestis (yp) is reported at 1.9 a resolution. the class iv ac fold is distinct from the previously described folds for class ii and class iii acs. the dimeric ac-iv folds into an antiparallel eight-stranded barrel whose connectivity has been seen in only three previous structures: yeast rna triphosphatase and two proteins of unknown function from pyrococcus furiosus and vibrio parahaemolyticus. eight highly conserved ionic ... | 2006 | 16905149 |
| identification of vibrio harveyi using pcr amplification of the toxr gene. | the aim of this study was to develop an effective method for the identification of vibrio harveyi based on using the toxr gene as a taxonomic marker. | 2006 | 16910927 |
| [isolation of thermostable direct hemolysin producing vibrio parahaemolyticus from food using screening by pcr in food-borne outbreaks]. | the producibility of thermostable direct hemolysin (tdh) is the most important pathogenic factor in vibrio parahaemolyticus. tdh (+) v. parahaemolyticus is usually isolated from patients having v. parahaemolyticus food-borne disease. tdh (+) v. parahaemolyticus is, however, very difficult to isolate from food and environmental samples. in the 5 years from 2000 to 2004 in tokyo, v. parahaemolyticus was isolated from food samples related to 67 of 227 v parahaemolyticus food-borne outbreaks. in the ... | 2006 | 16922481 |
| detection of pandemic vibrio parahaemolyticus o3:k6 serovar in gulf of mexico water and shellfish using real-time pcr with taqman fluorescent probes. | we describe a real-time multiplexed pcr method using taqman probes for the detection of total and pandemic vibrio parahaemolyticus o3:k6 serovar in oysters and gulf of mexico water (gulf water). the specificity of these primers and probes was tested for amplification of a 450 bp thermolabile hemolysin (tlh) and a 369 bp orf8 amplicon representing all v. parahaemolyticus and post-1996 clinical isolates of pandemic serovar o3:k6, respectively. the sensitivity of detection was 10 pg purified dna or ... | 2006 | 16923074 |
| bactericidal effects of wine on vibrio parahaemolyticus in oysters. | the bactericidal effects of wines on vibrio parahaemolyticus in oysters were studied to evaluate potential inactivation of v. parahaemolyticus in contaminated oysters by wine consumption. shucked whole oyster and oyster meat homogenate were inoculated with v. parahaemolyticus and mixed with red or white wine. survivals of v. parahaemolyticus in inoculated oysters were determined at 7 and 25 degrees c. populations of v. parahaemolyticus in inoculated whole oysters (5.52 log most probable number [ ... | 2006 | 16924905 |
| effects of electrolyzed oxidizing water treatment on reducing vibrio parahaemolyticus and vibrio vulnificus in raw oysters. | contamination of vibrio parahaemolyticus and vibrio vulnificus in oysters is a food safety concern. this study investigated effects of electrolyzed oxidizing (eo) water treatment on reducing v. parahaemolyticus and v. vulnificus in laboratory-contaminated oysters. eo water exhibited strong antibacterial activity against v. parahaemolyticus and v. vulnificus in pure cultures. populations of v. parahaemolyticus (8.74 x 10(7) cfu/ml) and v. vulnificus (8.69 x 10(7) cfu/ml) decreased quickly in eo w ... | 2006 | 16924906 |
| [study on the antibacterial mechanisms of copper-bearing montmorillonite]. | vibrio parahaemolyticus atcc 27519 was chosen as indicator of aquacultural pathogenic bacteria to determine the antibacterial activity and mechanism of copper-bearing montmorillonite (cu-mmt) in vitro. the results indicated that montmorillonite (mmt) had no antibacterial activity. the minimum inhibitory concentration (mic) and bactericidal concentration (mbc) of cu-mmt on vibrio parahaemolyticus were 75 and 300 mg/l, respectively. the activities of aspartate aminotransferase (ast), alanine amino ... | 2006 | 16933615 |
| purification and characterization of a lectin from the banana shrimp fenneropenaeus merguiensis hemolymph. | a lectin from the hemolymph of the banana shrimp fenneropenaeus merguiensis was purified by affinity chromatography on a fetuin-agarose column following by gel filtration on a superose-12 column. the native molecular mass of purified f. merguiensis lectin (fml) determined by gel filtration was 316.2 kda and its carbohydrate content was estimated to be 4.4%. by sds-page analysis, purified fml consisted of 32.3 kda and 30.9 kda subunits. these data suggest that this lectin is an oligomer. two-dime ... | 2007 | 16934939 |
| pore formation of thermostable direct hemolysin secreted from vibrio parahaemolyticus in lipid bilayers. | vibrio parahaemolyticus secretes thermostable direct hemolysin (tdh), a major virulence factor. earlier studies report that tdh is a pore-forming toxin. however, the characteristics of pores formed by tdh in the lipid bilayer, which is permeable to small ions, remain to be elucidated. ion channel-like activities were observed in lipid bilayers containing tdh. three types of conductance were identified. all the channels displayed relatively low ion selectivity, and similar ion permeability. the c ... | 2006 | 16940013 |
| response of vibrio parahaemolyticus to ethanol shock. | in this study, the growth and survival of vibrio parahaemolyticus in the presence of 0.0-8.0% ethanol was first examined. v. parahaemolyticus was then exposed to a sub-lethal dose of 5.0% ethanol for 30 and 60 min (ethanol shock). morphological changes and alterations in cell leakage, thermal tolerance at 47 degrees c, and susceptibility to 8% ethanol and low temperature (4 and -18 degrees c) of v. parahaemolyticus caused by ethanol shock were investigated. in addition, recoveries of the ethanol ... | 2006 | 16943038 |
| visible-light-induced bactericidal activity of a nitrogen-doped titanium photocatalyst against human pathogens. | the antibacterial activity of photocatalytic titanium dioxide (tio(2)) substrates is induced primarily by uv light irradiation. recently, nitrogen- and carbon-doped tio(2) substrates were shown to exhibit photocatalytic activities under visible-light illumination. their antibacterial activity, however, remains to be quantified. in this study, we demonstrated that nitrogen-doped tio(2) substrates have superior visible-light-induced bactericidal activity against escherichia coli compared to pure t ... | 2006 | 16957236 |
| quorum sensing-disrupting brominated furanones protect the gnotobiotic brine shrimp artemia franciscana from pathogenic vibrio harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates. | autoinducer 2 (ai-2) quorum sensing was shown before to regulate the virulence of vibrio harveyi towards the brine shrimp artemia franciscana. in this study, several different pathogenic v. harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates were shown to produce ai-2. furthermore, disruption of ai-2 quorum sensing by a natural and a synthetic brominated furanone protected gnotobiotic artemia from the pathogenic isolates in in vivo challenge tests. | 2006 | 16957276 |
| rapid estimation of microbial numbers in water using bulk fluorescence. | enumeration of microbial cells without culturing is an essential technique for microbial ecology and water quality evaluation. here we show that bulk fluorescence using the sybr gold dna stain can be used to rapidly quantify microbial cells per millilitre in fresh, marine and estuarine waters. the bulk fluorescence method is comparable to estimating cell concentrations in cultures using optical density; however, this enhanced method enables the user to estimate microbial numbers at lower concent ... | 2006 | 16958758 |
| preparation and use of media for protease-producing bacterial strains based on by-products from cuttlefish (sepia officinalis) and wastewaters from marine-products processing factories. | cuttlefish powder (cfp) from sepia officinalis by-products was prepared and tested as a fermentation substrate for microbial growth and protease production by several species of bacteria: bacillus licheniformis, bacillus subtilis, pseudomonas aeruginosa, bacillus cereus bg1, and vibrio parahaemolyticus. all microorganisms studied grew well and produced protease activity when cultivated in medium containing only cfp indicating that the strains can obtain their carbon and nitrogen source requireme ... | 2008 | 16962303 |
| development of monoclonal antibodies for simple identification of vibrio alginolyticus. | the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp. | 2006 | 16965376 |
| response of spinach and komatsuna to biogas effluent made from source-separated kitchen garbage. | recycling of kitchen garbage is an urgent task for reducing public spending and environmental burdens by incineration and/or landfill. there is an interesting regional effort in ogawa, saitama prefecture, japan, in which source-separated kitchen garbage is anaerobically fermented with a biogas plant and the resultant effluent is used as a quick-release organic fertilizer by surrounding farmers. however, scientific assessments of fertilizer values and risks in the use of the effluent were lacking ... | 2006 | 16973635 |
| bacterial lateral flagella: an inducible flagella system. | flagella are complex surface organelles that allow bacteria to move towards favourable environments and that contribute to the virulence of pathogenic bacteria through adhesion and biofilm formation on host surfaces. there are a few bacteria that possess functional dual flagella systems, such as vibrio parahaemolyticus, some mesophilic aeromonas spp., rhodospirillum centenum and azospirillum brasilense. these bacteria are able to express both a constitutive polar flagellum required for swimming ... | 2006 | 16978346 |
| vp1686, a vibrio type iii secretion protein, induces toll-like receptor-independent apoptosis in macrophage through nf-kappab inhibition. | vibrio parahaemolyticus, causative agent of human gastrointestinal diseases, possesses several virulent machineries including thermostable direct hemolysin and type iii secretion systems (ttss1 and -2). in this report, we establish that ttss1-dependent secretion and translocation of a v. parahaemolyticus effector protein vp1686 into the cytosol induces dna fragmentation in macrophages. we performed yeast two-hybrid screening to identify the molecules involved in vp1686-mediated cell death pathwa ... | 2006 | 16984916 |
| two type iv pili of vibrio parahaemolyticus play different roles in biofilm formation. | vibrio parahaemolyticus rimd2210633 has two sets of type iv-a pilus genes. one set is similar to that found in other gram-negative bacteria, such as pseudomonas aeruginosa, vibrio cholerae (chitin-regulated pilus; chirp), and vibrio vulnificus. the other is homologous to the genes for the mannose-sensitive hemagglutinin (msha) pilus. in this study, we analyzed the effects of the deletions in the pilin genes for each type iv pilus (the chirp and the msha pilus) on biofilm formation. although the ... | 2006 | 17020553 |
| tetrameric structure of thermostable direct hemolysin from vibrio parahaemolyticus revealed by ultracentrifugation, small-angle x-ray scattering and electron microscopy. | the thermostable direct hemolysin (tdh) is a major virulence factor of vibrio parahaemolyticus. we have characterized the conformational properties of tdh by small-angle x-ray scattering (saxs), ultracentrifugation and transmission electron microscopy. sedimentation equilibrium and velocity studies revealed that the protein is tetrameric in aqueous solvents. the guinier plot derived from saxs data provided a radius of gyration of 29.0 a. the elongated pattern with a shoulder of a pair distance d ... | 2007 | 17056060 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| characterization of a vibrio alginolyticus strain, isolated from alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus. | a vibrio strain isolated from alaskan oysters and classified by its biochemical characteristics as vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in vibrio parahaemolyticus. this trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of v. parahaemolyticus. this gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. to our knowledge, this is the first r ... | 2006 | 17056701 |
| antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure. | the relationship between the structure and antibacterial activity of 22 polyphenols was analyzed by using minimum inhibitory concentration (mic) as a criterion against 26 species of bacteria which can grow in mueller-hinton medium. there was no clear correlation between gram-staining and bacterial susceptibility to polyphenols, and the extent of the susceptibility was approximately dependent on the species of bacteria. in the same gram-negative bacteria, the antibacterial activity of the polyphe ... | 2006 | 17077519 |
| high amount of dinophysistoxin-3 in mytilus chilensis collected in seno de reloncaví, chile, during massive human intoxication associated with outbreak of vibrio parahaemolyticus. | this study describes the detection of high amount of 7-o-acyl-derivative dinophysistoxin-1 (dinophysistoxin-3) in filter bivalves collected on february 2005 in the seno de reloncaví, puerto montt city, southern chile, in the same period of time where an intoxication episode was associated with the presence of vibrio parahaemolyticus in shellfish. the diarrhetic shellfish poisoning (dsp) mouse bioassay of mussel extract samples, performed as described for regulatory testing, were negative to dsp ... | 2006 | 17077585 |
| efficacy of electrolysed oxidizing water in inactivating vibrio parahaemolyticus on kitchen cutting boards and food contact surfaces. | to determine the efficacy of electrolysed oxidizing (eo) water in inactivating vibrio parahaemolyticus on kitchen cutting boards and food contact surfaces. | 2006 | 17083714 |
| identification of vibrio isolates by a multiplex pcr assay and rpob sequence determination. | vibrio, a diverse genus of aquatic bacteria, currently includes 72 species, 12 of which occur in human clinical samples. of these 12, three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus-account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires 2 or more days to complete. to facilitate the identifi ... | 2007 | 17093013 |
| outbreak of gastroenteritis caused by the pandemic vibrio parahaemolyticus o3:k6 in mexico. | during 2003 and during late september of 2004, more than 1230 cases of gastroenteritis were reported in the south of sinaloa state, north-western mexico. all cases were attributed to the consumption of raw or undercooked shrimp collected at the huizache-caimanero lagunary system. vibrio parahaemolyticus was identified by standard biochemical methods, and many strains were positive for pcr amplifications of the tlh and tdh genes and negative for the trh gene. a representative strain belonged to t ... | 2006 | 17107421 |
| response of bdellovibrio and like organisms (balos) to the migration of naturally occurring bacteria to chemoattractants. | a dual culture-based and non-culture-based approach was applied to characterize predator bacterial groups in surface water samples collected from apalachicola bay, florida. chemotaxis drop assays were performed on concentrated samples in an effort to isolate predator bacteria by their chemotactic ability. yeast extract (ye) and casamino acids (ca) proved to be strong chemoattractants and resulted in three visibly distinct bands; however, dextrose, succinate, pyruvate, and concentrated cells of v ... | 2006 | 17115104 |
| involvement of the vibrio parahaemolyticus pvsc gene in export of the siderophore vibrioferrin. | the pvsc gene of unknown function has been found in the iron-regulated vibrioferrin biosynthesis operon of vibrio parahaemolyticus (tanabe, t. et al., j. bacteriol. 185: 6938-6949, 2003). the amino acid sequence deduced from the gene showed significant similarity to 12-transmembrane segment efflux pumps belonging to the major facilitator superfamily. a nonpolar deletion of pvsc in v. parahaemolyticus resulted in a reduced release of vibrioferrin into the medium. vibrioferrin release could be reg ... | 2006 | 17116982 |