| the dynamic processivity of the t4 dna polymerase during replication. | the polymerase (gp43) processivity during t4 replisome mediated dna replication has been investigated. the size of the okazaki fragments remains constant over a wide range of polymerase concentrations. a dissociation rate constant of approximately 0.0013 sec(-1) was measured for the polymerases from both strands, consistent with highly processive replication on both the leading and lagging strands. this processive replication, however, can be disrupted by a catalytically inactive mutant d408n gp ... | 2004 | 15148377 |
| [outcomes of radiation therapy for maxillary sinus carcinoma]. | we evaluated the outcome of radiation therapy for maxillary sinus carcinoma treated in our institution. | 2004 | 15148790 |
| [hormone production and secretion in thyroid tumors]. | thyroid epithelial cells can be classified into 2 types according to embryonic origin, those of foregut endoderm (follicular cells) and those of neuroectodermal deviation (c cells) origin. the production of tg, t4, and t3 is frequently evident with the immunoperoxidase methods is nontoxic thyroid tumors derived from follicular cells. in contrast, medullary thyroid carcinoma originated from thyroid c cells produces and secretes calcitonin. thyroid transcription factor-1 (ttf-1) regulates the expr ... | 2004 | 15148821 |
| effect of a test and control program for bovine johne's disease in victorian dairy herds 1992 - 2002. | to report on progress in johne's disease (jd) control in infected dairy herds participating in the victorian johne's disease test and control program (tcp). | 2004 | 15149075 |
| long-term results of a phase ii study of synchronous chemoradiotherapy in advanced muscle invasive bladder cancer. | we conducted a phase i/ii study investigating synchronous chemoradiotherapy with mitomycin c and infusional 5-fluorouracil (5-fu) in muscle invasive bladder cancer. early dose escalation results were previously published. we report the long-term toxicity and efficacy results with the optimised regimen. patients with muscle invasive bladder cancer with glomerular filtration rate >25 ml min(-1) were eligible. mitomycin (12 mg m(-2) on day 1 only) and infusional 5-fu (500 mg m(-2) day(-1)) for 5 da ... | 2004 | 15150587 |
| iron deficiency alters auditory recognition memory in newborn infants of diabetic mothers. | infants of diabetic mothers (idms) are at risk for perinatal brain iron deficiency that may target the developing hippocampus. the objective of this study was to evaluate hippocampally based recognition memory and infant development in idms with suspected brain iron deficiency (bid; cord ferritin </=34 microg/l) compared with idms with sufficient brain iron stores (bis; cord ferritin >34 microg/l) using event-related potentials (erps). erps assessed neonatal auditory cortical responses to sounds ... | 2004 | 15155871 |
| the value of endosonographic rectal carcinoma staging in routine diagnostics: a 10-year analysis. | endosonography is currently the gold standard for the local staging of rectal carcinoma, but its accuracy varies from 62% to 91%. this study aimed to determine the accuracy of endosonography, to evaluate the interobserver variability, and to compare the performance of the 7.5-mhz and the 10-mhz ultrasound scanners. | 2004 | 15156388 |
| thyroid disease: pathophysiology and diagnosis. | the thyroid is a butterfly-shaped gland that is located in the front of the neck just above the trachea; it weighs approximately 15 g to 20 g in the adult human. the thyroid produces and releases into the circulation at least two potent hormones, thyroxine (t4) and triiodothyronine (t3), that influence basal metabolic processes or enhance oxygen consumption in nearly all body tissues. thyroid hormones also influence linear growth; brain function, including intelligence and memory; neural develop ... | 2004 | 15157555 |
| maternal thyroid hormones early in pregnancy and fetal brain development. | during the last few decades our understanding of the possible role of thyroid hormones during brain development has increased and contributed to resolve previously discordant hypotheses, although much remains to be clarified. thyroid hormones of maternal origin are present in the fetal compartment, despite the very efficient uterine-placental 'barrier', necessary to avoid potentially toxic concentrations of free t4 and t3 from reaching fetal tissues before they are required for development. t3 r ... | 2004 | 15157838 |
| physiological and pathological aspects of the effect of human chorionic gonadotropin on the thyroid. | human chorionic gonadotropin (hcg) is a glycoprotein hormone that has structural similarity to tsh. at the time of the peak hcg levels in normal pregnancy, serum tsh levels fall and bear a mirror image to the hcg peak. this reduction in tsh suggests that hcg causes an increased secretion of t4 and t3. women with hyperemisis gravidarum often have high hcg levels that cause transient hyperthyroidsm. in the vast majority of such patients, there will be spontaneous remission of the increased thyroid ... | 2004 | 15157839 |
| mapping of functional sites on the primary structure of the contractile tail sheath protein of bacteriophage t4 by mutation analysis. | in order to determine the functional roles of amino acid residues in gp18 (gp: gene product), the contractile tail sheath protein of bacteriophage t4, the mutation sites and amino acid replacements of available and newly created missense mutants with distinct phenotypes were determined. amber mutants were also utilized for amino acid insertion by host amber suppressor cell strains. it was found that mutants that gave rise to a particular phenotype were mapped in a particular region along the pol ... | 2004 | 15158724 |
| t4 replication: what does "processivity" really mean? | | 2004 | 15159531 |
| [observation of clinical and pathological responses in short-term following immunochemotherapy for oral squamous cell carcinoma] | 24 patients with oral scc in stage t3 or t4 were randomly divided into two groups,and treated with two inductive therapies,13 cases of whom received an immumochemotherapy regimen(sapylin 15 ke+cddp 70mg/m(2)+vcr 1.4mg/m(2)+pym 80mg,s-pvp) and 11 cases,a chemotherapy regimen(cddp 90mg/m(2)+vcr 1.4mg/m(2)+pym 80mg,pvp) as control.the clinical response and pathological effect after immunochemotherapy or chemotherapy were evaluated through the clinical measurement of tumor size and pathological exam ... | 1997 | 15160216 |
| [subclinical hypothyroidism; the start of a clinical trial into the usefulness of treatment with radioactive iodine]. | subclinical hyperthyroidism is defined as the presence of serum free thyroxine (t4) and triiodothyronine (t3) levels within the reference range and a reduced serum thyrotrophin (tsh) level. evidence is accumulating that it has important clinical effects. randomised clinical trials are needed to answer the question whether or not treatment of subclinical hyperthyroidism prevents cardiac problems, especially atrial fibrillation, and preserves bone mineral density. a randomised, dutch multicentre t ... | 2004 | 15160563 |
| physiological and metabolic responses of wheelchair athletes in different racing classes to prolonged exercise. | the aims of this study were to examine and compare selected physiological and metabolic responses of wheelchair athletes in two paraplegic racing classes [t3: n = 8 (lesion levels t1-t7; paraplegics); t4: n = 9 (lesion levels below t7; paraplegics)] to prolonged exercise. in addition, we describe the responses of three tetraplegic athletes [t2: n = 3 (lesion levels c6/c7: tetraplegics)]. twenty athletes completed 90 min of exercise at 75% vo2peak on a motorized treadmill adapted for wheelchairs. ... | 2004 | 15160598 |
| increased nuclear factor-kb activation in human colorectal carcinoma and its correlation with tumor progression. | nuclear factor-kb (nf-kb) is a transcription factor that participates in the induction of several genes for cytokines and enzymes that play important functional roles in various cell types. the aim of this study was to determine nf-kb activation status in human colorectal carcinoma and its correlation to the clinicopathological characteristics of patients. | 2004 | 15161011 |
| sex and thyroid hormone status in women with rheumatoid arthritis: are there any effects of menopausal state and disease activity on these hormones? | there has been considerable interest in the role of hormones in the aetiopathogenesis of rheumatoid arthritis (ra). in this study, we aimed to investigate sex and thyroid hormone conditions according to menopausal state and disease activation in ra women. fifty-four women with ra were included in the study. age-matched 28 women with low back pain were used as controls. sex and thyroid hormones were evaluated in all patients, which included the measurement of estradiol (e2), progesterone, follicl ... | 2004 | 15161114 |
| identification of 18s ribosomal dna genotype of acanthamoeba from patients with keratitis in north china. | to identify the genotype of 18s ribosomal rna gene (18s rdna, rns) of the acanthamoeba strains isolated from patients with keratitis in northern china. | 2004 | 15161856 |
| repair rates of r-band, g-band and c-band dna in murine and human cultured cells. | repair of cyclobutane pyrimidine dimers (cpds) in cultured neonatal human fibroblasts and in mus spretus x m. castaneus f1 neonatal skin fibroblasts was analyzed after uvc-irradiation by cleavage with t4 endonuclease v cyclopyrimidine dimer glycosylase, alkaline-agarose gel electrophoresis, and southern blotting. the blots were sequentially probed with 32p-labeled alu, or b2, to preferentially illuminate r-band dna, by l1 to preferentially illuminate g-band dna, and by satellite dna to illuminat ... | 2004 | 15162013 |
| replacement therapy with levothyroxine plus triiodothyronine (bioavailable molar ratio 14 : 1) is not superior to thyroxine alone to improve well-being and cognitive performance in hypothyroidism. | there is evidence from recent controlled clinical studies that replacement therapy of hypothyroidism with t4 in combination with a small amount of t3 may improve the well-being of the patients. as the issue is still the subject of controversial discussion, our study was assigned to confirm the superiority of a physiological combination of thyroid hormones (absorbed molar ratio 14 : 1) over t4 alone with regard to mood states and cognitive functioning. | 2004 | 15163340 |
| [changes of thyroid hormone levels in the progression of coronary arteriosclerosis]. | the study concerned changes in the levels of thyroid hormones (t3, t4) and their proportional relations in the serum of peripheral venous blood of patients with ischemic heart disease (ihd) with reference to severity of coronary and myocardial insufficiency and clinical symptoms. thyroid hormones (th) were examined in patients with stenosing atherosclerosis of the coronary arteries with incipient angina pectoris (iap), acute myocardial infarction (ami), chronic ihd before and after mi and postin ... | 2004 | 15164504 |
| desferoxamine (dfo)--mediated iron chelation: rationale for a novel approach to therapy for brain cancer. | iron homeostasis is crucial to normal cell metabolism, and its deficiency or excess is associated with numerous disease states. the association of increased iron load with cancer may be due to several factors including free radical production, reduction of the body's protective mechanism to combat oxidative stress, inhibition of immune systems, inhibition of essential nutrient functions, facilitation of cancer growth, suppression of antitumor actions of macrophages, and lowering of the ratio of ... | 2004 | 15164994 |
| bacteriophage t4 endonuclease ii: concerted single-strand nicks yield double-strand cleavage. | in vivo, endonuclease ii (endoii) of coliphage t4 cleaves sites with conserved sequence elements (cses) to both the left and the right of the cleaved bonds, 16 bp altogether with some variability tolerated. in vitro, however, single-strand nicks in the lower strand predominate at sites containing only the left-side cse that determines the precise position of lower strand nicks. upper strand nick positions vary both in vivo and in vitro. a 24 bp substrate was nicked with the same precision as in ... | 2004 | 15165242 |
| design and crystal structure of bacteriophage t4 mini-fibritin nccf. | fibritin is a fibrous protein that forms "whiskers" attached to the neck of bacteriophage t4. whiskers interact with the long tail fibers regulating the assembly and infectivity of the virus. the fibritin trimer includes the n-terminal domain responsible for attachment to the phage particle and for the collar formation, the central domain forming a 500 a long segmented coiled-coil structure, and the c-terminal "foldon" domain. we have designed a "mini" fibritin with most of the coiled-coil domai ... | 2004 | 15165860 |
| involvement of multispecific organic anion transporter, oatp14 (slc21a14), in the transport of thyroxine across the blood-brain barrier. | the present study was aimed at investigating the involvement of mouse organic anion transporting polypeptide 14 (moatp14) in the uptake of t4 across the blood-brain barrier. functional expression of moatp14 in hek293 cells revealed that t4 and rt3 are high affinity substrates of moatp14 (michaelis constant, 0.34 and 0.46 microm, respectively), and the specific uptake of t3 was 4-fold less than that of t4 and rt3. taurocholate, probenecid, and estrone-3-sulfate were moderate inhibitors for moatp1 ... | 2004 | 15166123 |
| intraoperative hyperglycemia during infant cardiac surgery is not associated with adverse neurodevelopmental outcomes at 1, 4, and 8 years. | it is unknown whether intraoperative hyperglycemia in infants is associated with worse neurodevelopmental outcomes after low-flow cardiopulmonary bypass (lf), deep hypothermic circulatory arrest (ca), or both. | 2004 | 15166552 |
| correlation between the distribution of contrast medium and the extent of blockade during epidural anesthesia. | if the epidural spread of contrast medium can be well correlated with the spread of local anesthetics, epidurography can predict the dermatomal distribution of the anesthetic block. the authors evaluated the relation between radiographic and analgesic spread. | 2004 | 15166571 |
| meat intake and the recurrence of colorectal adenomas. | a large multicenter randomized controlled trial was re-assessed to check whether meat intake and a reduction in its consumption are associated with recurrence of adenomatous polyps of the large bowel, which are precursors of most colorectal malignancies. all subjects (n = 1905; 958 interventions and 947 controls) had one or more histologically confirmed colorectal adenomas removed during a colonoscopy within 6 months before randomization. the subjects were followed-up for approximately 4 years a ... | 2004 | 15167213 |
| elevated triiodothyronine in psychopathy - possible physiological mechanisms. | we assessed a total range of peripheral thyroid hormone fractions, binding globulins, and thyroid-active antibodies in 37 medication-free, violent or sexual offenders, aged 17-45 years, to describe possible mechanisms involved in the thyroid metabolism of aggressive men. the ratio between t3 and t4 correlated with ratings of psychopathy, indicating increased peripheral deiodination as a biological covariate to callous personality traits. autoimmune antibodies, hepatic failure, abnormal binding g ... | 2004 | 15168221 |
| temperature dependence of the energy gap of semiconductors in the low-temperature limit. | the temperature dependence of the electronic states and energy gaps of semiconductors is an old but still important experimental and theoretical topic. remarkably, extant results do not clarify the asymptotic t-->0 behavior. recent breakthroughs in the spectroscopy of enriched 28si allow us to measure changes in the band gap over the liquid 4he temperature range with an astounding precision of one part in 10(8), revealing a t4.0+/-0.2 decrease with increasing t. this is in excellent agreement wi ... | 2004 | 15169425 |
| escherichia coli nucleoside diphosphate kinase interactions with t4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. | in both prokaryotic and eukaryotic organisms, nucleoside diphosphate kinase is a multifunctional protein, with well defined functions in ribo- and deoxyribonucleoside triphosphate biosynthesis and more recently described functions in genetic and metabolic regulation, signal transduction, and dna repair. this paper concerns two unusual properties of nucleoside diphosphate (ndp) kinase from escherichia coli: 1) its ability to interact specifically with enzymes encoded by the virulent bacteriophage ... | 2004 | 15169771 |
| molecular classification of oral cancer by cdna microarrays identifies overexpressed genes correlated with nodal metastasis. | our purpose was to classify osccs based on their gene expression profiles, to identify differentially expressed genes in these cancers and to correlate genetic deregulation with clinical and histopathologic data and patient outcome. after conducting proof-of-principle experiments utilizing 6 hnscc cell lines, the gene expression profiles of 20 osccs were determined using cdna microarrays containing 19,200 sequences and the btsvq method of data analysis. we identified 2 sample clusters that corre ... | 2004 | 15170668 |
| [current status on laparoscopic surgery for colorectal cancer]. | the current status of laparoscopic surgery and its indications for colorectal cancer are described. according to multi-institutional registry by the japanese society of endoscopic surgeons, the number of laparoscopic surgeries for advanced colorectal cancer has been increasing during the last couple years. the short- and long-term results of laparoscopic surgery for pt1 or pt2 colon cancer are favourable, and laparoscopic surgery could be a standard procedure for such cases. however, the indicat ... | 2004 | 15170973 |
| elimination of proinflammatory cytokines in pediatric cardiac surgery: analysis of ultrafiltration method and filter type. | this study was undertaken to assess whether different filter types or ultrafiltration methods influence inflammatory markers in pediatric cardiac surgery. | 2004 | 15173725 |
| concentration-dependent regulation of thyrotropin receptor function by thyroid-stimulating antibody. | thyrotropin receptor (tshr) ab's of the stimulating variety are the cause of hyperthyroid graves disease. ms-1 is a hamster mab with tshr-stimulating activity. to examine the in vivo biological activity of ms-1, mice were treated with purified ms-1 intraperitoneally and the thyroid response evaluated. ms-1 induced a dose-dependent increase in serum thyroxine (t4), with a maximum effect after 10 proportional, variant g of ms-1 was administered. ms-1-secreting hybridoma cells were then transferred ... | 2004 | 15173885 |
| relationships between pcbs and thyroid hormones and retinol in female and male polar bears. | we studied the relationships between polychlorinated biphenyls (pcbs) and thyroid hormones (ths) and retinol within two groups of female polar bears (ursus maritimus), females with cubs of the year (fwcoy) and females without cubs of the year (fwocoy), and within a group of males. concentrations of five of the six quantified pcb congeners, i.e., pcb-99, pcb-153, pcb-156, pcb-180, pcb-194 (sigma pcb5), correlated with each other, whereas the concentrations of pcb-118 did not correlate with the ot ... | 2004 | 15175168 |
| effects of parity and body condition at parturition on endocrine and reproductive parameters of the cow. | the effect of parity (multiparous vs primiparous) and body condition score (bcs; <3.0 or > or =3.0, lean vs fat) at parturition on metabolic and endocrine profiles from 1 month before to 2 months after parturition were studied in 42 holstein cows grazing on improved pastures. bcs and milk production were determined every 2 weeks. non-esterified fatty acids (nefa), beta-hydroxy-butyrate (bhb), insulin, igf-i, leptin, thyroxine (t4) and 3,3',5-tri-iodothyroinine (t3) were determined in plasma ever ... | 2004 | 15175509 |
| [laparoscopic nephroureterectomy for upper urinary tract urothelial tumors]. | we present our 11 year experience with 15 laparoscopic nephroureterectomy procedures for upper urinary tract urothelial tumor. | 2004 | 15176373 |
| structural evidence of a passive base-flipping mechanism for beta-glucosyltransferase. | beta-glucosyltransferase (bgt) is a dna-modifying enzyme and a glycosyltransferase. this inverting enzyme transfers glucose from udp-glucose to the 5-hydroxymethyl cytosine bases of t4 phage dna. from previous structural analyses we showed that asp-100 and asn-70 were, respectively, the catalytic base and the key residue for specific dna recognition (larivière, l., gueguen-chaignon, v., and moréra, s. (2003) j. mol. biol. 330, 1077-1086). here, we supply biochemical evidence supporting their ess ... | 2004 | 15178685 |
| facial translocation approach in the management of central skull base and infratemporal tumors. | the objectives were to systematize the indications of various types of facial translocation and to present a technical modification to avoid some sequelae of this approach. | 2004 | 15179211 |
| [levels of unified metabolites and thyroid hormones in blood and oral fluid of children with minimal brain dysfunction]. | minimal brain dysfunctions in children with various perinatal complications are accompanied by metabolic imbalance manifested by decreased total protein content, the tendency to reduced triglycerides, increased cholesterol concentrations in the oral fluid, the trend to hypoproteinaemia, hypoglycaemia, hypotriglyceridaemia. the most significant changes in the redox systems alpha-ketoglutarate-glutamate, oxaloacetate-malate, pyruvate-lactate, dioxyacetone phosphate-alpha-glycerophosphate in biolog ... | 2004 | 15179829 |
| single-molecule spectroscopy studies of conformational change dynamics in enzymatic reactions. | protein conformational dynamics, often associated with static and dynamic inhomogeneities, plays a crucial role in biomolecular functions. it is extremely difficult to characterize such inhomogeneous dynamics in an ensemble-averaged measurement, especially when the protein involves in a multiple-step complex chemical reaction, such as an enzymatic reaction. single-molecule spectroscopy is a powerful approach to analyze protein conformational dynamics in real time under physiological conditions, ... | 2004 | 15180547 |
| the hypothalamic-pituitary-thyroid axis in preterm infants; changes in the first 24 hours of postnatal life. | the purpose of this study was to measure serum t4, free t4, tsh, t3, rt3, t4 sulfate, and thyroxine binding globulin at four time points within the first 24 h of life (cord and 1, 7, and 24 h) in infants between 24 and 34 wk gestation. the infants were subdivided into gestational age groups: 24-27 wk (n = 22); 28-30 wk (n = 26); and 31-34 wk (n = 24). the tsh surge in the first hour of postnatal life was markedly attenuated in infants of 24-27 wk gestation [8 compared with 20 (28-30 wk) and 23 m ... | 2004 | 15181064 |
| lipoprotein remnants and endothelial dysfunction in the postprandial phase. | the objective of this work was to study whether changes in remnant lipoprotein (rlp) plasma levels during the postprandial phase relate to alterations of the endothelial function. fasted patients (15 moderately dyslipidemic men) were given an oral fat load (ofl), and blood samples were collected before the ofl ingestion (t0) and 2, 4, 6, and 8 h (t2, t4, t6, t8) thereafter. endothelial function, determined as flow-mediated dilatation (fmd) of the brachial artery, was assessed at the same time po ... | 2004 | 15181082 |
| [construction, expression and antigenicity of bivalent vaccine candidate of human helicobacter pylori]. | to construct a recombinant vector containing fused gene of heat shock protein a(hspa) and outer membrane protein(omp) with m(r) 18,000, from human helicobacter pylori(hp) and express the fusion protein in e.coli bl21. | 2004 | 15182624 |
| expression of p63 (ta and deltan isoforms) in human primary well differentiated buccal carcinomas. | abnormalities in the p53 gene have been regarded as the most consistent genetic abnormalities detected in head and neck squamous cell carcinogenesis. two new members of the p53 gene family, p73 and p63, have recently been identified. we investigated the expression of the two n-terminal p63 isoforms (ta and deltan isoforms) in human primary well-differentiated buccal squamous cell carcinoma. both tap63 and deltanp63 isoforms were detected in the basal/suprabasal layers of all of the five specimen ... | 2004 | 15183414 |
| female sexual dysfunction after radical cystectomy: a new outcome measure. | to assess, in our contemporary radical cystectomy (rc) series, female sexual dysfunction and whether the type of diversion affected the occurrence of sexual dysfunction. outcome data after rc with and without orthotopic diversion has focused primarily on cure, urethral recurrence, and continence. | 2004 | 15183970 |
| historical sexual abuse and current thyroid axis profiles in women with premenstrual dysphoric disorder. | this study investigated whether women with premenstrual dysphoric disorder (pmdd) who also had histories of sexual abuse differed from women with pmdd with no previous sexual abuse and from women without pmdd in hypothalamic-pituitary-thyroid (axis measures). | 2004 | 15184704 |
| assessing thyroid hormone status in a patient with thyroid disease and renal failure: from theory to practice. | a 35-year-old asian male, treated for hyperthyroidism, systemic lupus erythematosis, and uremia presented with low serum total thyroxine (t4) and normal serum thyrotropin (tsh) levels. he had been receiving prednisone and methimazole for 15 weeks. free t4 measured by direct equilibrium dialysis was in the hypothyroid range (0.3 ng/dl; normal, 0.8-2.7). two possibilities were considered: (1) a weakly bound dialyzable inhibitor in uremic serum that interfered with this serum free t4 determination ... | 2004 | 15186619 |
| gene inactivation in the cyanobacterium synechococcus sp. pcc 7002 and the green sulfur bacterium chlorobium tepidum using in vitro-made dna constructs and natural transformation. | inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. this chapter describes how to generate such mutants of the cyanobacterium synechococcus sp. pcc 7002 and the green sulfur bacterium chlorobium tepidum by natural transformation with synthetic dna constructs. two alternative methods to generate the dna constructs, both performed entirely in vitro and based on the polymerase chain rea ... | 2004 | 15187290 |
| variations in lymphocyte activation during exercise testing in patients with coronary artery disease. | several studies have shown that strenuous exercise induces changes in the immune system. soluble interleukin-2 receptor (sil-2r) is a marker of immune system activation and is known to increase in association with cardiac disease. the aim of the present study was to assess sil-2r levels in patients with coronary artery disease (cad) in conjunction with exercise testing. | 2004 | 15187570 |
| biomechanical assessment of conventional unit rod fixation versus a unit rod pedicle screw construct: a human cadaver study. | fresh frozen human cadaver specimens were used to perform a biomechanical assessment focusing on spinal-pelvic fixation. unit rod fixation alone was compared with that of unit rod fixation supplemented with l5 pedicle screws. | 2004 | 15187631 |
| comparison of serum-free thyroxine concentrations determined by standard equilibrium dialysis, modified equilibrium dialysis, and 5 radioimmunoassays in dogs. | measurement of serum-free thyroxine (ft4) concentration provides a more accurate assessment of thyroid gland function than serum thyroxine (t4) or 3,5,3'-triiodothyronine (t3). techniques for measuring serum ft4 concentration include standard equilibrium dialysis (sed), radioimmunoassay (ria), and a combination of both (modified equilibrium dialysis [med]). this study compared results of serum ft4 measurements by means of sed, med, and 5 rias in 30 healthy dogs, 10 dogs with hypothyroidism, and ... | 2004 | 15188809 |
| effects of trimethoprim-sulfadiazine on thyroid function of horses. | trimethoprim-sulfadiazine was administered to horses in a randomized, placebo controlled study to determine the effects of potentiated sulfonamides on thyroid function in normal horses. the treatment group included eight horses that received trimethoprim-sulfadiazine mixed with molasses orally at 30 mg/kg once daily for eight weeks. the control group included 8 horses that received an oral placebo (flour mixed with molasses) once daily for the same period. thyroid function was evaluated prior to ... | 2004 | 15188829 |
| extended pneumonectomy for non small cell lung cancer--should we still do it? | the aim of the study was to assess the early and late results of extended pneumonectomies in lung cancer patients with t3 and t4 disease. between jan. 1995 and dec. 1999--445 pneumonectomies were performed in patients with lung cancer. in 37 patients without preoperative n2 involvement a standard pneumonectomy was extended to include the following additional resections: chest wall (10), pericardium (9), diaphragm (5), vcs (3), descending aorta (2), left atrium (5), esophagus (1) and tracheal bif ... | 2004 | 15190425 |
| sentinel lymph node mapping technique in colon cancer. | current conventional surgical and pathological techniques substantially understage colon cancer. this is evidenced by the fact that a significant subset of patients who are stage i and ii at the time of colectomy return with distant metastases and ultimately succumb to the disease within the next 5 years. the identification of more nodes within a specimen and the detailed analysis of lymph nodes with advanced pathological techniques such as immunohistochemistry and reverse-transcriptase polymera ... | 2004 | 15190495 |
| [dna ploidy and dna index in aneuploid laryngeal cancer]. | abnormal cellular dna content, a hallmark of malignancy, is known to be an important prognostic factor in many human solid tumors. in laryngeal tumors studies are not as common as in different locations. | 2004 | 15190598 |
| metabolic safety-margins do not differ between cows of high and low genetic merit for milk production. | three galactopoietic stimuli, frequent milking (4x), bovine somatotrophin (bst) and thyroxine (t4) were used in an additive stair-step design to achieve maximum output (metabolic capacity) in six peak-lactation cows of high genetic merit (ht) and six of low genetic merit (lt). a further six of each merit were untreated controls (hc, lc). milk yield was increased significantly by 4x, increased further by the combination of 4x and bst and increased further still and significantly by the full combi ... | 2004 | 15190941 |
| [association of upa and upar expression with invasive behaviors of urinary transitional cell carcinoma]. | urokinase-type plasminogen activator (upa)/its receptor (upar), serine protease system, plays a key role in the degradation of extracellular matrix and basement membranes, and intensifying the tumor invasion. the study was designed to investigate the expression of upa and upar in urinary transitional cell carcinoma. the correlation between their expression and tumor invasion was evaluated. | 2004 | 15191676 |
| characteristics and thyroid state-dependent regulation of iodothyronine deiodinases in pigs. | three iodothyronine deiodinases (d1, d2, and d3) regulate local and systemic availability of thyroid hormone. d1 and d2 activate the prohormone t4 to the thyromimetic t3, and d3 inactivates t4 and t3 to rt3 and 3,3'-diiodothyronine, respectively. the expression of the three deiodinases is tightly regulated with regard to developmental stage and cell type to provide fine tuning of t3 supply to target cells. most studies regarding distribution and regulation of deiodinases have been carried out in ... | 2004 | 15192045 |
| surgical outcome of combined pulmonary and atrial resection for lung cancer. | the study was carried out to assess the short and long-term outcome of patients with non-small cell lung cancer infiltrating the left atrium treated by surgery. | 2004 | 15192780 |
| thyroid cystadenoma, colloid goiter, and hypothyroidism in an american black bear (ursus americanus). | a 178-kg, 14-yr-old captive female american black bear (ursus americanus) was examined because of lethargy, inappetance, obesity, and alopecia. serum chemistry and complete blood count values were within normal limits. based on serum levels for total thyroxine (t4), free t4 by equilibrium dialysis (ft4ed), and canine thyroid-stimulating hormone concentrations, using assays validated for domestic dogs, hypothyroidism was diagnosed presumptively, and therapy with levothyroxine sodium (0.022 mg/kg ... | 2004 | 15193079 |
| effects of thyroxin therapy on cardiac function in patients with subclinical hypothyroidism: index of myocardial performance in the evaluation of left ventricular function. | we investigated the effects of thyroxine (t4) therapy on the cardiac function in subclinical hypothyroidism (sht) by using the index of myocardial performance (imp) and the conventional echocardiographic parameters. | 2004 | 15193811 |
| uvsx recombinase and dda helicase rescue stalled bacteriophage t4 dna replication forks in vitro. | the rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration often has been proposed as a major mechanism for accurately bypassing non-coding dna lesions. bacteriophage t4 encodes almost all of the proteins required for its own dna replication, recombination, and repair. both recombination and recombination repair in t4 rely on uvsx, a reca-like recombinase. we show here that uvsx plus the t4-encoded helicase dda suffice to ... | 2004 | 15194689 |
| [levothyroxine poisoning: clinical manifestations and therapeutic management]. | intoxication with l-tyroxine has a low prevalence, high prevalence is observed in pediatric age. toxic dose is unclear, asymptomatic patients have been observed with doses under 5 mg. systems with high prevalence of symptoms are cardiovascular system, sympathetic nervous system and gastrointestinal tube. symptoms could appear from hours to days after ingestion. early hours after l-tyroxine ingestion, levels of t4 and t3 are not related with severity. however, monitoring seric levels is necessary ... | 2004 | 15195485 |
| thyroid hormone alteration in pre-eclamptic women. | this study sought a possible relationship between pre-eclampsia and thyroid profile. in a case-control setting, total thyroxine (t4), total tri-iodothyronine (t3), free t4, free t3, thyroxine binding globulin (tbg) and thyrotropin (tsh) levels in 39 pre-eclamptic patients were measured and compared with the levels in 42 healthy controls. we examined possible variations with regard to the severity of pre-eclampsia by dividing cases into mild (n = 17) and severe (n = 22) subgroups. patients with m ... | 2004 | 15195501 |
| identification of essential residues within lit, a cell death peptidase of escherichia coli k-12. | bacteriophage exclusion is a suicide response to viral infection. in strains of escherichia coli k-12 infected with t4 phage this process is mediated by the host-encoded lit peptidase. lit is activated by a unique sequence in the major head protein of the t4 phage (the gol sequence) which then cleaves site-specifically the host translation factor ef-tu, ultimately leading to cell death. lit has very low sequence identity with other peptidases, with only a putative metallopeptidase motif, h(160)e ... | 2004 | 15196039 |
| [substitution of esophagus with stomach or colon without thoracotomy in the treatment of cervical esophageal carcinoma with laryngeal function preserved]. | to study the feasibility and effect of substituting esophagus with stomach or colon without thoracotomy in the treatment of cervical esophageal carcinoma with laryngeal function preserved. | 2004 | 15196444 |
| breast conservation after neoadjuvant chemotherapy: the md anderson cancer center experience. | to determine patterns of local-regional recurrence (lrr) and ipsilateral breast tumor recurrence (ibtr) among patients treated with breast conservation therapy after neoadjuvant chemotherapy. | 2004 | 15197191 |
| incidence of vertebral deformities in 255 female rheumatoid arthritis patients measured by morphometric x-ray absorptiometry. | to date, no studies have been published on incident deformities in patients with rheumatoid arthritis (ra). morphometric x-ray absorptiometry (mxa) is an alternative to conventional x-rays for identifying vertebral deformities. the aim of the present study was to describe the incidence of vertebral deformities in 255 female ra patients measured by mxa, and the relationship between incident deformities and clinical and demographic variables. mxa is still under evaluation for its ability to identi ... | 2005 | 15197538 |
| impact of fdg-pet for staging of oesophageal cancer. | treatment of oesophageal cancer depends on staging and the general health of the patient. in stages i-ii b, as well as in some stage iii diseases, surgical resection remains the therapy of choice for cure, but a curative approach is not possible in stage iv. in our hospital we give preoperative radio-chemotherapy to all patients with an oesophageal cancer t>1, nx, m0. therefore, the main purpose of the clinical staging of oesophageal cancer is the exclusion of m1 and t4 disease with infiltration ... | 2004 | 15197549 |
| american society of clinical oncology recommendations on adjuvant chemotherapy for stage ii colon cancer. | to address whether all medically fit patients with curatively resected stage ii colon cancer should be offered adjuvant chemotherapy as part of routine clinical practice, to identify patients with poor prognosis characteristics, and to describe strategies for oncologists to use to discuss adjuvant chemotherapy in practice. | 2004 | 15199089 |
| gastric cancer surgery: morbidity and mortality results from a prospective randomized controlled trial comparing d2 and extended para-aortic lymphadenectomy--japan clinical oncology group study 9501. | radical gastrectomy with regional lymphadenectomy is the only curative treatment option for gastric cancer. the extent of lymphadenectomy, however, is controversial. the two european randomized trials only reported an increase in operative morbidity and mortality, but failed to show survival benefit, in the d2 lymphadenectomy group. we conducted a randomized controlled trial to compare the japanese standard d2 and d2 + para-aortic nodal dissection. | 2004 | 15199090 |
| idiopathic spinal cord hernia. | idiopathic spinal cord hernia (isch) is a rare cause of slowly progressive spinal cord dysfunction. it is readily diagnosed on magnetic resonance imaging of the spine. the neurological deficit related to isch is often reversible once surgical exploration and reduction of the hernia is achieved. we present the case of a 37 year-old lady with a ventral isch at the t4 level. there was a significant post-operative improvement in her myelopathy post-operatively. isch should be considered in the diffe ... | 2004 | 15200221 |
| surgical treatment of pancoast tumours. | due to its localisation in the apex of the lung with invasion of the lower part of the brachial plexus, first ribs, vertebrae, subclavian vessels or stellate ganglion, a superior sulcus tumour causes characteristic symptoms, like arm or shoulder pain or horner's syndrome. if rib invasion is the only feature, lysis of the rib must be evident on the chest radiograph; otherwise the tumour cannot be defined as a pancoast tumour. it is important to adequately stage the tumour, because staging signifi ... | 2004 | 15201002 |
| promotion of thyroid tumors in rats by pregnenolone-16alpha-carbonitrile (pcn) and polychlorinated biphenyl (pcb). | pregnenolone-16alpha-carbonitrile (pcn) and aroclor 1254 (pcb) both reduce serum thyroid hormone levels in rats, but only pcn consistently produces an increase in serum thyrotropin (tsh). pcn-mediated increases in tsh result in increased thyroid follicular cell proliferation and hyperplasia, which may represent early events on a morphological continuum leading to neoplasia. the purpose of this study was to assess whether pcn, a compound that increases serum tsh, and pcb, which does not increase ... | 2004 | 15201439 |
| simultaneous determination of endogenous and 13c-labelled thyroid hormones in plasma by stable isotope dilution mass spectrometry. | this study describes a capillary gas chromatography-mass spectrometry (gc-ms) method for the simultaneous determination of endogenous thyroid hormone (thyroxine, t4) and its 13c-labelled analogue (13c6-thyroxine) in plasma. 13c9-thyroxine was used as analytical internal standard. a double derivatization (ch3oh/hcl and hfba) inducing good gc mobility was used for the gc-ms analysis of the thyroid hormones. quantification was carried out by selected ion monitoring (sim) of specific ions of the fra ... | 2004 | 15203028 |
| a rat neurodevelopmental evaluation of offspring, including evaluation of adult and neonatal thyroid, from mothers treated with ammonium perchlorate in drinking water. | the purpose of this study was to evaluate the potential neurodevelopmental toxicity of perchlorate exposure during gestation and the first 10 days of lactation. mated sprague-dawley rats (25/exposure group) were given continual access to 0, 0.1, 1.0, 3.0, or 10.0 mg/kg-day ammonium perchlorate (ap) in drinking water, starting gestation day 0 (mating) through lactation day 10 (dl 10). one pup/sex/litter/exposure group was assigned to (1) juvenile brain weights, morphometry, and neuropathology; (2 ... | 2004 | 15204722 |
| direct labeling of rna with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes. | direct labeling of rna is an expedient method for labeling large quantities (e.g. micrograms) of target rna for microarray analysis. we have developed an efficient labeling system that uses t4 rna ligase to attach a 3'-biotinylated donor molecule to target rna. microarray analyses indicate that directly labeled rna is uniformly labeled, has higher signal intensity than comparable labeling methods and achieves high transcript detection sensitivity. the labeled donor molecule we have developed all ... | 2004 | 15205470 |
| the pcna-rfc families of dna clamps and clamp loaders. | the proliferating cell nuclear antigen pcna functions at multiple levels in directing dna metabolic pathways. unbound to dna, pcna promotes localization of replication factors with a consensus pcna-binding domain to replication factories. when bound to dna, pcna organizes various proteins involved in dna replication, dna repair, dna modification, and chromatin modeling. its modification by ubiquitin directs the cellular response to dna damage. the ring-like pcna homotrimer encircles double-stran ... | 2004 | 15210332 |
| preoperative uracil, tegafur, and concomitant radiotherapy in operable rectal cancer: a phase ii multicenter study with 3 years' follow-up. | to assess tolerance and efficacy of preoperative treatment with uracil/tegafur and radiotherapy (rt) followed by surgery and postoperative flurouracil (fu)/leucovorin (lv) in patients with rectal cancer. | 2004 | 15210740 |
| should female patients undergoing parathyroid-sparing total thyroidectomy receive routine prophylaxis for transient hypocalcemia? | hypocalcemia following parathyroid-sparing total thyroidectomy is frequent and can prolong hospital stay. we undertook this study to identify preoperative predictors of postoperative hypocalcemia useful in the postoperative management of these patients. we examined patients undergoing total thyroidectomy for benign disease with preservation of at least three parathyroids from january 2000 to january 2001. low serum calcium was considered below 8.0 mg/dl. age, gender, preoperative serum calcium, ... | 2004 | 15212410 |
| head circumference and linear growth during the first 3 years in treated congenital hypothyroidism in relation to aetiology and initial biochemical severity. | to determine the head circumference and linear growth in children with congenital hypothyroidism (ch) during the first 3 years of life in relation to the aetiology of ch and initial biochemical severity of thyroid function. | 2004 | 15212659 |
| consequences of thyroxine treatment on diaphragm and edl of normal and dystrophic hamsters. | previously administration of thyroxine (t4) to dystrophic hamsters improved ventilation and slowed the progression of the disease. we hypothesized that the normalization of ventilation in these animals was due to t4 improving structural and functional characteristics of the diaphragm. in the present study, contractile characteristics of the diaphragm and the extensor digitorum longus (edl) from normal and dystrophic hamsters were evaluated after two months of t4 treatment. compared to their plac ... | 2004 | 15213735 |
| familial thyroxin-binding globulin excess with ichthyosis: a case report. | thyroxin (t4) binding globulin (tbg) the major thyroid hormone transport protein in humans. congenital or acquired problems lead to tbg excess. inheritance of tbg excess follows an x-linked pattern. a 21-month-old boy with ichthyosis was referred to the pediatric endocrinology clinic with high levels of thyroid hormones (tt3 = 325 ng/dl, tt4 23 microg/dl, ft3 = 3.49 pg/dl, ft4 = 1.44 ng/dl, tsh = 2.48 microiu/ml). he was clinically euthyroidic. thyroid gland was normal in size and homogeneous. t ... | 2004 | 15214750 |
| [subarachnoid anesthesia for cesarean section in a patient with ehlers-danlos syndrome type ii]. | a 32 year-old woman with full-term pregnancy and a history of ehlers-danlos syndrome type ii underwent cesarean delivery owing to breech presentation and the presence of oligohydramnios. anesthesia was provided with a subarachnoid block with 6.5 mg of bupivacaine and 32.5 microg of fentanyl to t4. depth of anesthesia was adequate and hemodynamic stability was good. the intervention was uneventful and the outcomes were excellent for both mother and fetus. a subarachnoid block with low doses of lo ... | 2004 | 15214762 |
| in vitro and in vivo bacteriolytic activities of escherichia coli phages: implications for phage therapy. | four t4-like coliphages with broad host ranges for diarrhea-associated escherichia coli serotypes were isolated from stool specimens from pediatric diarrhea patients and from environmental water samples. all four phages showed a highly efficient gastrointestinal passage in adult mice when added to drinking water. viable phages were recovered from the feces in a dose-dependent way. the minimal oral dose for consistent fecal recovery was as low as 10(3) pfu of phage per ml of drinking water. in co ... | 2004 | 15215109 |
| antioxidants modulate thyroid hormone- and noradrenaline-induced dna damage in human sperm. | the genotoxic effects of steroidal oestrogens are probably brought about by metabolic changes in their phenolic groups accompanied by the generation of quinones and reactive oxygen species. although non-steroidal oestrogens and related compounds have not been thoroughly investigated for genotoxicity, some of them also contain phenolic groups that could be involved in redox cycling. therefore, the aim of the present study was to evaluate the possible dna-damaging effects of the thyroid hormones t ... | 2004 | 15215333 |
| [biopsy technique and biopsy schemes for a first series of prostatic biopsies]. | to define the modalities of prostatic biopsies in patients with suspected prostate cancer, particularly concerning prevention of complications, the number of biopsies and the biopsy schemes ensuring an optimal cancer detection rate, and recording of prognostic elements, all with an acceptable morbidity, | 2004 | 15217126 |
| [clinical and histological prognostic factors of renal cancer with caval thrombus]. | the objective of this study was to determine the clinical and histological factors influencing survival of patients with renal cancer and caval thrombus. | 2004 | 15217128 |
| simultaneous development of bilateral retrobulbar and intradural spinal masses following donor lymphocyte infusions for acute lymphoblastic leukemia relapsing after bone marrow transplantation. | the authors describe a 6-year-old boy with acute lymphoblastic leukemia in relapse after bone marrow transplantation. complete remission was obtained after reinduction chemotherapy followed by donor lymphocyte infusions. ten months later, right-sided proptosis and exotropia developed, with back pain and leg paralysis. computed tomography revealed bilateral orbital masses. magnetic resonance imaging demonstrated abnormal signal intensity in the t4-6 vertebral bodies and an intradural mass on the ... | 2004 | 15218421 |
| delayed traumatic spinal epidural hematoma with spontaneous resolution of symptoms. | post-traumatic spinal epidural hematomas are uncommon, usually requiring emergent neurosurgical evacuation. we present a symptomatic delayed post-traumatic epidural hematoma of the t-spine that resolved within hours of administration of high dose steroids. a 22-year-old man presented 10 days after sustaining blunt trauma during a motor vehicle crash. he developed signs of acute cord compression with loss of sensation and motor function in bilateral lower extremities with priapism. magnetic reson ... | 2004 | 15219302 |
| effects of triiodothyronine on 5-ht(1a) and 5-ht(1b) autoreceptor activity, and postsynaptic 5-ht(1a) receptor activity, in rat hypothalamus: lack of interaction with imipramine. | triiodothyronine (t3) is effective in both augmenting and accelerating the therapeutic response to antidepressant drugs, especially tricyclics, and there is evidence from both human and animal studies that it acts on serotonergic neurotransmission. in this work we examined the effects of t3 alone and together with imipramine on 5-ht levels in the hypothalamus and on 5-ht(1a) and 5-ht(1b) autoreceptor sensitivity, using in vivo microdialysis in the rat. the effects of t3 on postsynaptic 5-ht(1a) ... | 2004 | 15219641 |
| evidence that intravenous vasopressors can affect rostral spread of spinal anesthesia in pregnancy. | the authors have previously observed an apparent association between rostral spread of spinal anesthesia and choice of intravenous vasopressor given to maintain maternal systolic arterial pressure during cesarean delivery. this study tested the hypothesis that an intravenous infusion of phenylephrine can reduce rostral spread of spinal anesthesia in pregnancy, compared with ephedrine. | 2004 | 15220768 |
| role of the aging vasculature and erb b-2 signaling in epidermal growth factor-dependent intravasion of breast carcinoma cells. | the risks for developing breast carcinoma and dying from the disease increase with age. mortality from breast carcinoma usually is due to metastatic disease. metastatic cells are able to invade into the vascular tissue in a growth factor-dependent manner. because breast carcinoma mortality increases with age, examination of breast carcinoma interactions with young and aged endothelial cells is essential. | 2004 | 15222007 |
| independent mitochondrial origin and historical genetic differentiation in north eastern asian cattle. | in order to clarify the origin and genetic diversity of cattle in north eastern asia, this study examined mitochondrial displacement loop sequence variation and frequencies of bos taurus and bos indicus y chromosome haplotypes in japanese, mongolian, and korean native cattle. in mitochondrial analyses, 20% of mongolian cattle carried b. indicus mitochondrial haplotypes, but japanese and korean cattle carried only b. taurus haplotypes. in contrast, all samples revealed b. taurus y chromosome hapl ... | 2004 | 15223036 |
| holliday junction binding and resolution by the rap structure-specific endonuclease of phage lambda. | rap endonuclease targets recombinant joint molecules arising from phage lambda red-mediated genetic exchange. previous studies revealed that rap nicks dna at the branch point of synthetic holliday junctions and other dna structures with a branched component. however, on x junctions incorporating a three base-pair core of homology or with a fixed crossover, rap failed to make the bilateral strand cleavages characteristic of a holliday junction resolvase. here, we demonstrate that rap can mediate ... | 2004 | 15223317 |
| quantitative microfluidic separation of dna in self-assembled magnetic matrixes. | we present an experimental study of the microfluidic electrophoresis of long dna in self-assembling matrixes of magnetic bead columns. results are presented for the rapid separation of lambda-phage, 2lambda-dna, and bacteriophage t4 dna, where separation resolutions greater than 2 between lambda and t4 are achieved in times as short as 150 s. the use of a computer-piloted flow control system and injection results in high reproducibility between separations. we compare the experimentally measured ... | 2004 | 15228353 |
| identification and staging of pancreatic tumours using computed tomography, endoscopic ultrasound and mangafodipir trisodium-enhanced magnetic resonance imaging. | pancreatic malignancy can be staged by a number of different investigations, either alone or in combination. the purpose of the present study was to compare the use of endoscopic ultrasound, ct and mangafodipir trisodium-enhanced mri for the staging of pancreatic malignancy, particularly with respect to determining resectability prior to surgery. twenty-seven patients referred for the investigation of a suspected pancreatic malignancy were entered into the trial. all patients had contrast-enhanc ... | 2004 | 15230749 |
| effects of oral ibandronate administered daily or intermittently on fracture risk in postmenopausal osteoporosis. | oral daily (2.5 mg) and intermittent ibandronate (between-dose interval of >2 months), delivering a similar cumulative exposure, were evaluated in 2946 osteoporotic women with prevalent vertebral fracture. significant reduction in incident vertebral fracture risk by 62% and 50%, respectively, was shown after 3 years. this is the first study to prospectively show antifracture efficacy for the intermittent administration of a bisphosphonate. | 2004 | 15231010 |