| [effect of long-term exposure to low dose gamma-irradiation on the rat thyroid status]. | the effect of long-term exposure to low-dose external radiation on the rat thyroid status was studied. the experiments were carried out on wistar female rats. the single doses absorbed were 0.1; 0.25; 0.5 gy. the rats were irradiated 20 times (5 days x 4 weeks). the animals were decapitated after 1, 30 and 180 days following the last irradiation. blood serum was assayed for content of thyroxin (t4) and triiodothyronine (t3) radioimmunologically. the liver was spectrophotometrically assayed for t ... | 2004 | 15060946 |
| human dna polymerase-eta, an a-t mutator in somatic hypermutation of rearranged immunoglobulin genes, is a reverse transcriptase. | we have proposed previously that error-prone reverse transcription using pre-mrna of rearranged immunoglobulin variable (igv) regions as templates is involved in the antibody diversifying mechanism of somatic hypermutation (shm). as patients deficient in dna polymerase-eta exhibit an abnormal spectrum of shm, we postulated that this recently discovered y-family polymerase is a reverse transcriptase (rt). this possibility was tested using a product-enhanced rt (pert) assay that uses a real time p ... | 2004 | 15061777 |
| the crystal structure of the uvsw helicase from bacteriophage t4. | in bacteriophage t4, the wxy system repairs dna damage by a process that involves homologous recombination. this system comprises three proteins, the reca-like recombination protein uvsx, a recombination mediator protein uvsy, and a helicase uvsw. here we report the 2.0 a resolution crystal structure of the n-terminal two domains of the uvsw helicase (uvswnf; residues 1-282). the structure reveals a typical helicase reca-like domain linked to a small n-terminal alpha/beta domain that likely bind ... | 2004 | 15062081 |
| carcinoma of the oral pharynx: an analysis of subsite treatment heterogeneity. | the data indicate that scc of the various subsites of the oropharynx can be treated successfully with acceptable locoregional control and survival rates by using either surgery or primary radiotherapy for ti or t2 primary lesions. treatment success data for late-stage disease (t3 and t4) are less encouraging. regardless of which modality is used or which treatment center is administering treatment. this finding may suggest an intrinsic property of these lesions or the patient that may be going u ... | 2004 | 15062362 |
| pion treatment of prostate carcinoma at paul scherrer institute (formerly swiss institute for nuclear research (sin)) from 1983 to 1992. | - between 1983 and 1992 a total of 55 patients suffering from prostate cancer were treated with pions (pi-mesons) at the paul scherrer institute in villigen, switzerland. this form of therapy was used at only three centers world wide on 245 patients with this diagnosis. since the last project in vancouver, canada, was closed in 1994, this form of therapy has been relegated to the realm of history. | 2004 | 15063876 |
| polychlorinated biphenyls (pcbs) exert thyroid hormone-like effects in the fetal rat brain but do not bind to thyroid hormone receptors. | polychlorinated biphenyls (pcbs) are ubiquitous environmental contaminants routinely found in human and animal tissues. developmental exposure to pcbs is associated with neuropsychologic deficits, which may be related to effects on thyroid hormone (th) signaling in the developing brain. however, pcbs may interfere with th signaling solely by reducing circulating levels of th, or they may exert direct effects on th receptors (trs). therefore, we tested whether maternal exposure to a commercial pc ... | 2004 | 15064154 |
| effects of organochlorine contaminants on thyroid hormone levels in arctic breeding glaucous gulls, larus hyperboreus. | studies on glaucous gulls (larus hyperboreus) breeding in the barents sea have reported that high blood levels of halogenated organic contaminants in this species might cause reproductive, behavioral, and developmental stress. however, potential endocrine system modulation caused by contaminant exposure has yet not been reported in this arctic apical predator. in this present study we aimed to investigate whether the current levels of a selection of organochlorines (ocs) were associated with alt ... | 2004 | 15064156 |
| impact of polychlorinated biphenyl (aroclor 1254) and vitamin c on antioxidant system of rat ventral prostate. | to evaluate the effect of polychlorinated biphenyls (pcb) and vitamin c on ventral prostatic antioxidant system in adult male rats. | 2004 | 15064829 |
| impact of t and n stage and treatment on survival and relapse in adjuvant rectal cancer: a pooled analysis. | to determine survival and relapse rates by t and n stage and treatment method in five randomized phase iii north american rectal adjuvant studies. | 2004 | 15067027 |
| cerivastatin demonstrates enhanced antitumor activity against human breast cancer cell lines when used in combination with doxorubicin or cisplatin. | competitive inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme a (hmg-coa) reductase are commonly used in the clinic to treat hypercholesterolemia and have been reported to exert antitumor effects. cerivastatin is a novel, synthetic and the most pharmacologically potent inhibitor of hmg-coa reductase. we decided to examine the cytostatic/cytotoxic activity of cerivastatin against human breast cancer cell lines and to test whether the effects of cerivastatin could be potentiated by doxorubicin and ... | 2004 | 15067336 |
| influence of thyroxine on serum soluble interleukin-2 receptor alpha levels in thyroid disorders. | activation of cell-mediated immunity by soluble interleukin-2 receptor alpha (sil-2ralpha) release is well documented. the aim of this study was to measure serum concentrations of sil-2ralpha in patients with autoimmune and non-autoimmune thyroid disorders in different stages of thyroid function, before and after administration of l-thyroxine (l-t4) and its discontinuation as well as before and during methimazole administration. | 2004 | 15068404 |
| artifactually elevated serum-free thyroxine levels measured by equilibrium dialysis in a pregnant woman with familial dysalbuminemic hyperthyroxinemia. | familial dysalbuminemic hyperthyroxinemia (fdh) is a familial autosomal dominant syndrome caused by abnormal albumin with an increased affinity for thyroxine (t4). two types of mutations in the albumin gene, replacing the normal arginine 218 with a histidine (r218h) or a proline (r218p), have been reported to cause fdh. here, we report a pregnant japanese woman with fdh caused by the mutant albumin r218p. she had extremely elevated total t4 levels but normal tsh. while the majority of t4was boun ... | 2004 | 15068631 |
| rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop. | protein engineers have widely adopted directed evolution as a design algorithm, but practitioners have not come to a consensus about the best method to evolve protein molecular recognition. we previously used dna shuffling to direct the evolution of escherichia coli beta-glucuronidase (gus) variants with increased beta-galactosidase activity. epistatic (synergistic) mutations in amino acids 557, 566, and 568, which are part of an active site loop, were identified in that experiment (matsumura, i ... | 2004 | 15069062 |
| thyroid function and pregnancy status in broodmares. | to determine whether thyroid function was associated with pregnancy status in broodmares. | 2004 | 15070060 |
| molecular architecture of the prolate head of bacteriophage t4. | the head of bacteriophage t4 is a prolate icosahedron with one unique portal vertex to which the phage tail is attached. the three-dimensional structure of mature bacteriophage t4 head has been determined to 22-a resolution by using cryo-electron microscopy. the t4 capsid has a hexagonal surface lattice characterized by the triangulation numbers t(end) = 13 laevo for the icosahedral caps and t(mid) = 20 for the midsection. hexamers of the major capsid protein gene product (gp)23* and pentamers o ... | 2004 | 15071181 |
| establishment of mrfp1 as a fluorescent marker in aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (gateway). | the advent of fluorescent proteins as vital dyes had a major impact in many research fields. different green fluorescent protein (gfp) variants were established in prokaryotic and eukaryotic organisms within the past 10 years, and other fluorescent proteins were discovered and applied. we expressed the discosoma red fluorescent protein, dsred (t4), the improved monomeric red fluorescent protein (mrfp1) and the blue fluorescent protein (bfp) in the filamentous fungus aspergillus nidulans. whereas ... | 2004 | 15071756 |
| [an analysis of dna content and cell cycle in nasopharyngeal carcinoma]. | to evaluate the relationship between the dna content, cell cycle and the clinical stages of nasopharyngeal carcinoma (npc). | 2004 | 15071919 |
| a novel dna topoisomerase inhibitor: dehydroebriconic acid, one of the lanostane-type triterpene acids from poria cocos. | traditional chinese medicinal plants are a treasure house for screening novel inhibitors of dna polymerases and dna topoisomerases from mammals; in the present study, nine lanostane-type triterpene acids were found in sclerotium of poria cocos. among the nine compounds, only dehydroebriconic acid could potently inhibit dna topoisomerase ii (topo ii) activity (ic(50) = 4.6 microm), while the compound moderately inhibited the activities of dna polymerases alpha, beta, gamma, delta, epsilon, eta, i ... | 2004 | 15072595 |
| effect of thyroid hormone on uncoupling protein-3 mrna expression in rat heart and skeletal muscle. | thyroid hormones (triiodothyronine [t3] and thyroxine [t4]) stimulate ucp-3 expression in skeletal muscle. we examined whether thyroid hormone-induced changes in uncoupling protein (ucp)-3 mrna expression are related to directs effects of t3 or reflect secondary effects of the hormone through stimulation of renin-angiotensin or beta-adrenergic systems. hyperthyroidism was produced by three injections of 100 microg t3/100 g body weight on alternate days with or without concomitant treatment with ... | 2004 | 15072699 |
| are bioequivalence studies of levothyroxine sodium formulations in euthyroid volunteers reliable? | levothyroxine (lt4) has a narrow therapeutic index. consequently, precise standards for assessing the bioequivalence of different lt4 products are vital. we examined the methodology that the food and drug administration (fda) recommends for comparing the bioavailability of lt4 products, as well as three modifications to correct for endogenous, thyroxine (t4) levels, to determine if the methodology could distinguish lt4 products that differ by 12.5%, 25%, or 33%. with no baseline correction for t ... | 2004 | 15072701 |
| strategies for precise quantification of transgene expression levels over several generations in rice. | variation in transgene expression levels can result from uncontrolled differences in experimental protocols. studies conducted over generations could, by their design, generate additional unwanted variation. to study sources of spurious variation, transgene expression levels were quantified over five homozygous generations in two independent transgenic rice lines created by particle bombardment. both lines contained the same gus expression unit and had been shown to exhibit stable inheritance of ... | 2004 | 15073211 |
| osteoporotic cytokines and bone metabolism on rats with induced hyperthyroidism; changes as a result of reversal to euthyroidism. | hyperthyroidism is characterized by increased bone turnover and resorptive activity. raised levels of serum osteoporotic cytokines, such as interleukin (il) -1beta, il-6 and tumor necrosis factor (tnf)-alpha have been demonstrated previously in hyperthyroidism. these elevations are controversial and it is difficult to differentiate the contribution of thyroid hormones to the elevation of cytokines from that of the autoimmune inflammation in graves' disease (gd) and follicular cell damage in thyr ... | 2003 | 15074839 |
| breast cancer in iran: results of a multi-center study. | breast cancer is the most common cancer in iranian women. this study aims to demonstrate the characteristics of breast diseases- and especially breast cancer- according to pathologic records in tehran, iran. | 2004 | 15075000 |
| endocrine profile and physical stature of children with perthes disease. | children with perthes disease have been thought to be of smaller stature than their peers. no reproducible evidence exists to demonstrate an endocrinopathy as the cause. this study reviewed 139 children with perthes disease. height and weight were recorded and compared with standardized growth charts. a blood sample was analyzed for thyroid stimulating hormone (tsh), thyroxin (t4), and insulin-like growth factor 1 (igf1). two urine samples were collected at 8 am and 8 pm within 24 hours. from th ... | 2004 | 15076600 |
| radical external beam radiotherapy for prostate cancer in japan: preliminary results of the changing trends in the patterns of care process survey between 1996-1998 and 1999-2001. | to report the preliminary results of a study to delineate the changing trends in radical external beam radiotherapy usage for prostate cancer between the 1996-1998 and 1999-2001 survey periods in japan. | 2004 | 15078908 |
| elicitation of neutralizing antibodies with dna vaccines expressing soluble stabilized human immunodeficiency virus type 1 envelope glycoprotein trimers conjugated to c3d. | dna vaccines expressing the envelope (env) of human immunodeficiency virus type 1 (hiv-1) have been relatively ineffective at generating high-titer, long-lasting immune responses. oligomeric or trimeric (gp140) forms of env that more closely mimic the native proteins on the virion are often more effective immunogens than monomeric (gp120) envelopes. in this study, several forms of env constructed from the hiv-1 isolate yu-2 (hiv-1(yu-2)) were tested for their immunogenic potential: a trimeric fo ... | 2004 | 15078953 |
| [lipid abnormalities in elderly patients with subclinical hyperthyroidism]. | sensitive thyroid-stimulating hormone (tsh) assays provide identification of many patients with subclinical hyperthyroidism resulting from excessive production or excessive replacement of thyroid hormone. subclinical hyperthyroidism is defined by a tsh below normal (suppressed) with normal serum t3 and t4 levels. subclinical hyperthyroidism is the goal of thyroid hormone therapy in patients with thyroid cancer, solitary thyroid nodules, multinodular or diffuse goiters, or a history of head and n ... | 2003 | 15080051 |
| selective binding to transthyretin and tetramer stabilization in serum from patients with familial amyloidotic polyneuropathy by an iodinated diflunisal derivative. | in familial amyloidotic polyneuropathy, ttr (transthyretin) variants are deposited as amyloid fibrils. it is thought that this process involves ttr tetramer dissociation, which leads to partially unfolded monomers that aggregate and polymerize into amyloid fibrils. this process can be counteracted by stabilization of the tetramer. several small compounds, such as diclofenac, diflunisal and flufenamic acid, have been reported to bind to ttr in vitro, in the t4 (thyroxine) binding channel that run ... | 2004 | 15080795 |
| comparative endocrinology of cycling and non-cycling asian (elephas maximus) and african (loxodonta africana) elephants. | up to 14% of asian and 29% of african elephants in captivity are not cycling normally or exhibit irregular cycles based on progestin profiles. to determine if ovarian acyclicity is related to other disruptions in endocrine activity, serum pituitary, thyroid, adrenal, and ovarian hormones in weekly samples collected for 6-25 months were compared between normal cycling (n=22 each species) and non-cycling (n=6 asian; n=30 african) elephants. a subset of cycling females (n=4 asian, 7 african) also w ... | 2004 | 15081836 |
| plasma thyroid hormone pattern in king penguin chicks: a semi-altricial bird with an extended posthatching developmental period. | plasma concentrations of thyroid hormones (th) were investigated during the extended posthatching developmental period (approximately 11 months) of a semi-altricial bird species, the king penguin (aptenodytes patagonicus). the first period of growth in summer was marked by a progressive rise in plasma t4 concentration that paralleled rapid increases in body mass and in structural and down growth. by contrast, plasma t3 concentration had already reached adult levels in newly hatched chicks and di ... | 2004 | 15081840 |
| hypoxia-inducible factor 1 alpha in high-risk breast cancer: an independent prognostic parameter? | hypoxia-inducible factor 1 alpha (hif-1alpha) furnishes tumor cells with the means of adapting to stress parameters like tumor hypoxia and promotes critical steps in tumor progression and aggressiveness. we investigated the role of hif-1alpha expression in patients with node-positive breast cancer. | 2004 | 15084243 |
| mutations of bacteriophage t4 59 helicase loader defective in binding fork dna and in interactions with t4 32 single-stranded dna-binding protein. | bacteriophage t4 gene 59 protein greatly stimulates the loading of the t4 gene 41 helicase in vitro and is required for recombination and recombination-dependent dna replication in vivo. 59 protein binds preferentially to forked dna and interacts directly with the t4 41 helicase and gene 32 single-stranded dna-binding protein. the helicase loader is an almost completely alpha-helical, two-domain protein, whose n-terminal domain has strong structural similarity to the dna-binding domains of high ... | 2004 | 15084598 |
| rna substrate specificity and structure-guided mutational analysis of bacteriophage t4 rna ligase 2. | here we report that bacteriophage t4 rna ligase 2 (rnl2) is an efficient catalyst of rna ligation at a 3'-oh/5'-po(4) nick in a double-stranded rna or an rna.dna hybrid. the critical role of the template strand in approximating the reactive 3'-oh and 5'-po(4) termini is underscored by the drastic reductions in the rna-sealing activity of rnl2 when the duplex substrates contain gaps or flaps instead of nicks. rna nick joining requires atp and a divalent cation cofactor (either mg or mn). neither ... | 2004 | 15084599 |
| classification of pancreatic cancer: comparison of japanese and uicc classifications. | the japan pancreas society (jps) published the fifth japanese edition of the general rules for the study of pancreatic cancer in 2002, the same year that the union internationale contre le cancer (uicc) published its sixth edition. the jps fifth edition had been revised, based on data from 18,629 cases of carcinoma of the pancreas registered by the jps (1981-1996) with the goal of making the general rules simple, easy to understand, and reliable for predicting outcome. the 2 classifications are ... | 2004 | 15084962 |
| [colorectal cancer: what should be the management of primary tumour?]. | colon and rectal cancer are in france a challenging problem in public health, reaching the second place in cancer related deaths. surgery is still the key of curative treatment. crude survival increased from 33% to 55% during twenty years. colonoscopy is the referent exam for positive diagnosis. in rectal cancer, endorectal ultrasound and mri have optimised local staging. the quality of general staging is important since it could change therapeutic strategy. spiral ct scan is now probably superi ... | 2004 | 15086058 |
| a moderate and transient deficiency of maternal thyroid function at the beginning of fetal neocorticogenesis alters neuronal migration. | epidemiological studies and case reports show that even a relatively minor degree of maternal hypothyroxinemia during the first half of gestation is potentially dangerous for optimal fetal neurodevelopment. our experimental approach was designed to result in a mild and transient period of maternal hypothyroxinemia at the beginning of corticogenesis. normal rat dams received the goitrogen 2-mercapto-1-methyl-imidazole for only 3 d, from embryonic d 12 (e12) to e15. maternal thyroid hormones decre ... | 2004 | 15087434 |
| cesium-137 needle brachytherapy boosts after external beam irradiation for locally advanced carcinoma of the tongue and floor of the mouth. | a retrospective study was undertaken to compare local tumor control and complications of external beam radiation therapy with and without interstitial 137cs needle brachytherapy boost doses. | 2002 | 15090274 |
| low hepatic 7-ethoxyresorufin-o-deethylase (erod) activity and minor alterations in retinoid and thyroid hormone levels in flounder (platichthys flesus) exposed to the polychlorinated biphenyl (pcb) mixture, clophen a50. | the effect of the polychlorinated biphenyl (pcb) mixture clophen a50 on hepatic cytochrome p4501a1 dependent erod (7-ethoxyresorufin-o-deethylase) activity, plasma thyroid hormone levels and plasma, kidney and liver retinoid concentrations of the euryhaline flatfish flounder (platichthys flesus) was determined 2 and 10 days after i.p. (intraperitoneal) injection with 20, 100 and 500 mg clophen a50/kg body weight. no effect of clophen a50 on total cytochrome p450 content in flounder liver was obs ... | 1996 | 15091378 |
| the bacteriophage t4 dna injection machine. | the tail of bacteriophage t4 consists of a contractile sheath surrounding a rigid tube and terminating in a multiprotein baseplate, to which the long and short tail fibers of the phage are attached. upon binding of the fibers to their cell receptors, the baseplate undergoes a large conformational switch, which initiates sheath contraction and culminates in transfer of the phage dna from the capsid into the host cell through the tail tube. the baseplate has a dome-shaped sixfold-symmetric structu ... | 2004 | 15093831 |
| primary tumor volume of nasopharyngeal carcinoma: prognostic significance for local control. | to study the prognostic significance of primary tumor volume on local control of nasopharyngeal carcinoma. | 2004 | 15093895 |
| pilot study of postoperative reirradiation, chemotherapy, and amifostine after surgical salvage for recurrent head-and-neck cancer. | salvage surgery alone after radiotherapy (rt) failure for locally advanced head-and-neck cancer is frequently unsuccessful because of subsequent recurrence. we designed a prospective protocol to determine the feasibility, toxicity, and preliminary efficacy of a regimen of postoperative reirradiation, chemotherapy and the radioprotector amifostine after salvage head-and-neck surgery. | 2004 | 15093901 |
| a combination of t3, and t4 for hypothyroidism? | | 2004 | 15095532 |
| mechanical measurement of single-molecule binding rates: kinetics of dna helix-destabilization by t4 gene 32 protein. | bacteriophage t4 gene 32 protein (gp32) is a single-stranded dna (ssdna) binding protein, and is essential for dna replication, recombination and repair. while gp32 binds preferentially and cooperatively to ssdna, it has not been observed to lower the thermal melting temperature of natural double-stranded dna (dsdna). however, in single-molecule stretching experiments, gp32 significantly destabilizes lambda dna. in this study, we develop a theory of the effect of the protein on single dsdna stre ... | 2004 | 15095865 |
| ammonium perchlorate effects on thyroid function and growth in bobwhite quail chicks. | bobwhite quail chicks were used to investigate ammonium perchlorate (ap; nh4clo4) effects on thyroid function and growth. beginning at 3 to 4 d posthatch, we evaluated organismal thyroid status (circulating hormones), activation of the hypothalamic-pituitary-thyroid axis (thyroid wt) and thyroidal hormone content over a wide range of ap concentrations (50 microg/l - 4,000 mg/l) in drinking water, for relatively short (2-week) and longer (8-week) exposures. thyroidal thyroxine (t4) content, the m ... | 2004 | 15095897 |
| comparative value of attachment measurements in implant dentistry. | in implant dentistry, the level of bone attachment is normally assessed by clinical and radiologic parameters. in the literature, however, the accuracy of these measurements has been a source of controversy. the purpose of this study was to assess the reliability of attachment measurements in the beagle dog model. | 2004 | 15101591 |
| identification of micrornas and other tiny noncoding rnas by cdna cloning. | micrornas (mirnas) and other small rnas can be identified by cloning and sequencing cdnas prepared from the approximately 22-nt fraction of total rna. methods are described for the construction of cdna libraries from small noncoding rnas through the use of t4 rna ligase, reverse transcriptase, and polymerase chain reaction. cdnas are cloned in lambda or plasmid vectors, and the sequences are compared to annotated genomic sequence databases, and analyzed by rna folding programs to distinguish mir ... | 2004 | 15103073 |
| x-ray structural and simulation analysis of a protein mutant: the value of a combined approach. | the effect of the mutation arg 96 to his on the stability of bacteriophage t4 lysozyme has been previously studied by calorimetric experiments, x-ray crystallography, and free energy simulation techniques. the experimental and calculated values for the difference between the free energy of denaturation of the mutant and the wild type are in reasonable agreement. however, the two approaches led to different explanations for the loss in stability. to analyze the differences, a series of refinement ... | 2004 | 15103635 |
| thyroid autoantibody profiles in ophthalmic dominant and thyroid dominant graves' disease differ and suggest ophthalmopathy is a multiantigenic disease. | thyroid-associated ophthalmopathy (tao) occurs in 25-50% of patients with graves' disease (gd) and is occasionally seen in hypothyroid hashimoto's disease or euthyroid individuals. the link between tao and hyperthyroidism remains unclear. we hypothesized that qualitative or quantitative differences in thyroid antibodies might determine individual predisposition to these features. | 2004 | 15104563 |
| prevention of bone loss in ovariectomized rats: the effect of salvia miltiorrhiza extracts. | the preventive effect of salvia miltiorrhiza extracts (smes) on the progress of bone loss induced by ovariectomy (ovx) was studied in rats. we measured body weight and bone histomorphometry in sham, ovx or smes-administered ovx rats. from light microscopic analyses, a porous or erosive appearances were observed on the surface of trabecular bone of tibia in ovx rats, whereas those of the same bone in sham rats and in smes-administered rats were composed of fine particles. the trabecular bone area ... | 2004 | 15106738 |
| solvent interactions and protein dynamics in spin-labeled t4 lysozyme. | aspects of t4 lysozyme dynamics and solvent interaction are investigated using atomically detailed molecular dynamics (md) simulations. two spin-labeled mutants of t4 lysozyme are analyzed (t4l-n40c and t4l-k48c), which have been found from electronic paramagnetic resonance (epr) experiments to exhibit different mobilities at the site of spin probe attachment (n- and c-terminus of helix b, respectively). similarities and differences in solvent distribution and diffusion around the spin label, as ... | 2004 | 15106997 |
| chronic glutamine supplementation increases nasal but not salivary iga during 9 days of interval training. | oral glutamine supplementation during and after exercise abolishes exercise-induced decreases in plasma glutamine concentration but does not affect secretory iga (siga) salivary output. whether chronic glutamine supplementation during high-intensity interval training influences salivary and nasal siga concentration is unknown. the purpose of this study was examine the effects of chronic glutamine supplementation on siga during intense running training. runners (n = 13, body mass 69.9 +/- 2.8 kg, ... | 2004 | 15107413 |
| iodine deficiency and development of brain. | iodine is a trace element essential for the synthesis of triodothyronine (t3) and thyroxine (t4). inadequate intake of iodine leads to insufficient production of these hormones, which play a vital role in the process of early growth and development of most organs, especially the brain. the neurological sequele of iodine deficiency are mediated by thyroid hormone deficiency, varying from minimal brain function to a syndrome of severe intellectual disability. all the basic processes of neurogenesi ... | 2004 | 15107513 |
| squamous cell carcinoma of the anal margin. | to define the optimal treatment of patients with squamous cell carcinoma (scca) of the anal margin. | 2004 | 15112245 |
| novel tshbeta subunit gene mutation causing congenital central hypothyroidism in a newborn male. | newborn screening programs that use only high tsh levels as a marker for hypothyroidism may overlook neonates with congenital hypothyroidism (ch) due to tsh deficiency. we sought the cause of tsh deficiency in a neonate with low levels of thyroxine and tsh. the coding region of the tshbeta gene was amplified and its sequence examined for mutations. two mutations in exon 3 were identified: 1) a nucleotide deletion of t410 in codon 105 resulting in a frameshift in one allele, and 2) a previously u ... | 2004 | 15112912 |
| thyroid function in clinical subtypes of major depression: an exploratory study. | : | 2004 | 15113438 |
| angiogenic factors in squamous cell carcinoma of the oral cavity: do they have prognostic relevance? | angiogenesis is a cascade-like mechanism which is essential for tumour growth and metastasis. therefore the existence of angiogenic molecules and the density of activated endothelial cells in individual tumours is of major interest. material/patients: in order to evaluate the prognostic significance of these molecules, the distribution pattern was studied of vascular endothelial growth factor (vegf) and activated endothelial cells in tumours and normal, healthy oral mucosal specimens from 51 con ... | 2004 | 15113577 |
| characterization of bacteriophage t3 dna ligase. | dna ligases of bacteriophage t4 and t7 have been widely used in molecular biology for decades, but little is known about bacteriophage t3 dna ligase. here is the first report on the cloning, expression and biochemical characterization of bacteriophage t3 dna ligase. the polyhistidine-tagged recombinant t3 dna ligase was shown to be an atp-dependent enzyme. the enzymatic activity was not affected by high concentration of monovalent cations up to 1 m, whereas 2 mm atp could inhibit its activity by ... | 2004 | 15113838 |
| can acceleromyography detect low levels of residual paralysis? a probability approach to detect a mechanomyographic train-of-four ratio of 0.9. | the incidence of residual paralysis, i.e., a mechanomyographic train-of-four (tof) ratio (t4/t1) less than 0.9, remains frequent. routine acceleromyography has been proposed to detect residual paralysis in clinical practice. although acceleromyographic data are easy to obtain, they differ from mechanomyographic data, with which they are not interchangeable. the current study aimed to determine (1) the acceleromyographic tof ratio that detects residual paralysis with a 95% probability, and (2) th ... | 2004 | 15114208 |
| isotope dilution tandem mass spectrometric method for t4/t3. | the thyroid hormones thyroxine (t4) and 3,5,3'-triidothyronine (t3) are essential for regulating a number of biological processes, including growth, neurodevelopment, carbohydrate metabolism, oxygen consumption and protein synthesis. immunoassays are the current methods for thyroid hormone measurement and suffer from a lack of specificity. our objective was to simultaneously measure t4 and t3 using isotope dilution tandem mass spectrometry within a single run. to compare the results obtained by ... | 2004 | 15115693 |
| urinary beta-2 microglobulin in renal dysfunction associated with hypothyroidism. | hypothyroidism is associated with a number of functional renal disorders primarily affecting water and salt handling. in this study, we aim to investigate functional renal disorders in overt and subclinical hypothyroidism. we utilised urinary beta-2 microglobulin (beta2m) as a sensitive marker of tubular dysfunction. urinary beta2m excretion and tests for renal functions were repeated before and after t4 replacement therapy. forty-four patients (28 with overt disease, 16 with subclinical disease ... | 2004 | 15117089 |
| proangiogenic action of thyroid hormone is fibroblast growth factor-dependent and is initiated at the cell surface. | the effects of thyroid hormone analogues on modulation of angiogenesis have been studied in the chick chorioallantoic membrane model. generation of new blood vessels from existing vessels was increased 3-fold by either l-thyroxine (t4; 10(-7) mol/l) or 3,5,3'-triiodo-l-thyronine (10(-9) mol/l). t4-agarose reproduced the effects of t4, and tetraiodothyroacetic acid (tetrac) inhibited the effects of both t4 and t4-agarose. tetrac itself was inactive and is known to block actions of t4 on signal tr ... | 2004 | 15117822 |
| aggressive surgical treatment for t4 gastric cancer. | surgical treatment for locally advanced gastric cancer remains controversial, and many still question the benefits of extended resection. the aim of this study was to evaluate the effectiveness of combined resection of the involved organs with regard to survival in patients with gastric cancer. between 1993 and 2000, among the 1638 patients with gastric cancer who underwent gastrectomy, 82 were found to have evidence of adjacent organ spread at laparotomy. a retrospective analysis of these patie ... | 2004 | 15120372 |
| genetic and pathologic significance of 1p, 17p, and 18q aneusomy and the erbb2 gene in colorectal cancer and related normal colonic mucosa. | among chromosome defects in colon cancer, deletions in 1p, 17p, and 18q have been reported as frequent events. to verify this, we investigated 1p, 17p, and 18q aneusomy in 60 colorectal cancers and their surrounding mucosa by means of fluorescence in situ hybridization (fish). we also evaluated erbb2 gene (alias her-2/neu) amplification in a subset of tumors. the genetic picture in tumors was correlated with chromosomal alterations in normal colonic mucosae, as well with clinicopathologic variab ... | 2004 | 15120910 |
| nonthyroidal illness syndrome in patients with subarachnoid hemorrhage due to intracranial aneurysm. | we have previously reported that subarachnoid hemorrhage due to ruptured intracranial aneurysm (sh) is associated with changes in the hormonal profile in the first 24 hours after the event. we proposed that the hormonal changes observed are due to the intense stress to which the patients are exposed. however, the thyroidal hormonal profile is indicative of the presence of a nonthyroidal illness syndrome (ntis). in this paper, we examined whether the change in the thyroid hormone profile is compa ... | 2004 | 15122429 |
| promising results with chemoradiation in patients with sinonasal undifferentiated carcinoma. | sinonasal undifferentiated carcinoma (snuc) is an uncommon malignancy associated with poor prognosis. the optimal treatment approach for snuc has not been established was performed. | 2004 | 15122660 |
| [bilateral breast cancer. incidence and risk factors]. | to clarify, thanks to a retrospective study of 24 bilateral breast cancer cases, the frequency, the risk factors and the prognosis of bilateral breast cancers. | 2004 | 15123135 |
| nitric oxide and thyroid gland: modulation of cardiovascular function in autonomic-blocked anaesthetized rats. | we have previously reported that acute administration of n(g)-nitro-l-arginine methyl ester (l-name) increases the mean arterial pressure (map) and heart rate (hr) in autonomic-blocked (cab) anaesthetized rats. in the present study we examined whether thyroid and adrenal glands are involved in these pressor and chronotropic responses. sprague-dawley rats were studied after bilateral vagotomy and ganglionic blockade with hexamethonium (10 mg kg(-1)), and stabilization of map with infusion of phen ... | 2004 | 15123566 |
| endogenous tsh in the diagnosis of hypothyroidism in dogs. | to determine whether measurement of canine thyrotropin (ctsh) would aid in the diagnosis of hypothyroidism, serum samples of 65 dogs with clinical signs suggestive of hypothyroidism were evaluated. diagnosis was confirmed in 26 dogs and excluded in 39 dogs based on tsh-stimulation testing. total thyroxine (t4) was significantly lower and ctsh significantly higher in hypothyroid dogs compared to euthyroid dogs. canine tsh was above (> 0.6 ng/ml) in 15 (57.7%) and below the upper limit of the refe ... | 2004 | 15124775 |
| [functional properties of sphagnum magellanicum fiber and its direct use in formulation of bakery products]. | characterization of functional properties of sphagnum magellanicum fiber were investigated. water absortion (wac) and water retention (wrc) capacities, swelling capacity (sc); organic molecule absortion capacity (omac) and cationic interchange capacity (cic) were evaluated, as well as its incorporation as fiber source to bakery produts. diferent particles sizes were selected to evaluate their effects on the functional properties of moss fiber: t1(1.4 mm);t2(1.0 mm); t3(0.42 mm); t4(0.18 mm). bes ... | 2003 | 15125083 |
| [multiple myeloma and hiv/aids infection. three case reports]. | hiv/aids infection rages at the endemic state in sub saharan african and especially in our country. we are reporting 3 observations of patients, all of females affected by hiv/aids and multiple's myeloma. they are all in-patients in the department of haematology, university hospital of brazzaville, from 2000 to 2002. in two cases out of three, multiple's myeloma is discovered after the tracking of hiv/aids infection. in the other case, the tracking hiv/aids seems to be posterior to the multiple' ... | 2004 | 15125359 |
| c-kit expression in sarcomatoid renal cell carcinoma: potential therapy with imatinib. | sarcomatoid (s) renal cell carcinoma (rcc) is an uncommon subtype of rcc with a poor prognosis because of its local aggressiveness and high metastatic rate. currently, there is no specific, effective treatment for it. a relatively nontoxic tyrosine kinase inhibitor, imatinib (sti-571) has been approved as a target therapy in neoplasms that express c-kit. we investigated c-kit expression in this type of tumor, which to our knowledge has not been previously described. | 2004 | 15126780 |
| bicalutamide 150 mg maintains bone mineral density during monotherapy for localized or locally advanced prostate cancer. | we evaluated changes in bone mineral density (bmd), fat-free mass (ffm) and serum lipid levels during bicalutamide 150 mg monotherapy compared with medical castration for 2 years. | 2004 | 15126801 |
| bacteriophage-based genetic system for selection of nonsplicing inteins. | a genetic selection system that detects splicing and nonsplicing activities of inteins was developed based on the ability to rescue a t4 phage strain with a conditionally inactive dna polymerase. this phage defect can be complemented by expression of plasmid-encoded phage rb69 dna polymerase. insertion of an intein gene into the active site of the rb69 dna polymerase gene renders polymerase activity and phage viability dependent on protein splicing. the effectiveness of the system was tested by ... | 2004 | 15128583 |
| analysis of screw placement relative to the aorta and spinal canal following anterior instrumentation for thoracic idiopathic scoliosis. | axial computed tomographic (ct) evaluation of the position of anterior vertebral body screws placed thoracoscopically in patients with adolescent idiopathic scoliosis (ais). | 2004 | 15129071 |
| evidence for developmental hypopituitarism in ill preterm infants. | the relationship between the concentrations of cortisol and t4 with outcome in the preterm infants has not been well studied. | 2004 | 15129226 |
| a comparative study on diagnostic differentials of t3 and t4 hormones for thyroid disorders. | results of thyroid function tests of 671 individuals have been studied. the analysis shows that t3 and t4 hormone tests are not efficacious as compared to tsh test (p < 0.001) and in diagnosing hypothyroid cases t3 and t4 lead to almost same results while in detecting hyperthyroid cases t3 is superior to t4 (p < 0.01). | 2003 | 15129853 |
| phage t4 early promoters are resistant to inhibition by the anti-sigma factor asia. | phage t4 early promoters are transcribed in vivo and in vitro by the escherichia coli rna polymerase holoenzyme esigma(70). we studied in vitro the effects of the t4 anti-sigma(70) factor asia on the activity of several t4 early promoters. in single-round transcription, promoters motb, denv, mrh.2, mota wild type and up element-deleted mota are strongly resistant to inhibition by asia. the alpha-c-terminal domain of esigma(70) is crucial to this resistance. dnase i footprinting of esigma(70) and ... | 2004 | 15130121 |
| chronic cardiac-specific thyrotoxicosis increases myocardial beta-adrenergic responsiveness. | whereas many cardiac symptoms of thyrotoxicosis resemble those of the hyperadrenergic state, circulating catecholamines are reduced or normal in this condition. to test the hypothesis that the thyrotoxic heart is hypersensitive to catechol-amines, we studied beta-adrenergic signaling in a transgenic (tg) mouse in which the human type 2 iodothyronine deiodinase (d2) gene is expressed in myocardium. because d2 converts t4 to t3, the active form of thyroid hormone, the d2 tg mouse exhibits mild, ch ... | 2004 | 15131256 |
| molecular and physiological evaluation of subtropical environmental isolates of acanthamoeba spp., causal agent of acanthamoeba keratitis. | previous molecular examination of acanthamoeba spp. has resulted in the determination of distinct genotypes in this genus (designated t1-t12, t14). genotype t4 has been responsible for the majority of cases of acanthamoeba keratitis. here we examine the relative abundance of environmental t4 isolates on beaches and ask whether they have temperature and salinity tolerances that could enhance pathogenicity. twenty-four acanthamoeba strains were isolated from beach sand (n = 20), soil (n = 3), and ... | 2004 | 15134255 |
| dna mutagenesis using t4 dna polymerase and dpni restriction endonuclease. | | 2004 | 15136180 |
| neoadjuvant chemoradiotherapy for locally advanced esophageal cancer: experience at a single institution. | patients receiving neoadjuvant chemoradiotherapy followed by surgery (crs) undergo downstaging of their tumor and have improved survival when compared with patients undergoing surgery followed by adjuvant chemoradiotherapy (scr). | 2004 | 15136354 |
| the impact of cardiopulmonary bypass on selenium status, thyroid function, and oxidative defense in children. | selenium has important functions for oxidative defense and thyroid hormone metabolism. selenium-dependent enzymes include 5'-iodothyronine deiodinase and glutathione peroxidase (gpx). the objective of this study was to investigate the relationship between plasma selenium, gpx activity, and thyroid hormone status in pediatric cardiac surgical patients. plasma concentrations of selenium, free triiodothyronine (ft3), free thyroxin (ft4), and c-reactive protein as well as plasma activity of gpx were ... | 2004 | 15136904 |
| effect of dosing and sampling time on serum thyroxine, free thyroxine, and thyrotropin concentrations in dogs following multidose etodolac administration. | thirty-eight dogs with orthopedic disorders received etodolac, an nsaid, at 10.0 to 13.3 mg/kg po once daily for 14 to 19 days. mean total thyroxine (t4), free thyroxine (ft4), and canine thyrotropin (ctsh) values before and after etodolac administration were compared using paired t-tests. a significant (p <.05) decrease in t4 values occurred after etodolac administration with 21% of these values falling below the reference range. a significant (p <.05) increase in ctsh following etodolac admini ... | 2003 | 15136976 |
| effects of arginine vasopressin (avp) on the pituitary-thyroid axis in the rat: evidence that endogenous avp, acting via v1 receptors, lowers tsh blood concentration. | we have investigated the effects of the prolonged administration (2 or 8 days) of arginine-vasopressin (avp), alone or with antagonists of its v1 and v2 receptors (v1-ra and v2-ra), on the rat pituitary-thyroid axis. in the 8-day, but not 2-day experiments, avp raised thyroid weight, and the effect was prevented by v1-ra. morphometry showed that the avp-induced increase in thyroid weight was mainly due to a rise in the stroma volume. in the 2-day, but not 8-day experiments, avp lowered tsh plasm ... | 2004 | 15138627 |
| evaluation of downstaging as leading concept in sphincter-saving surgery for rectal cancer after preoperative radio-chemotherapy (preop rct). | to evaluate the downstaging of rectal cancer after preop r +/- ct. | 2003 | 15139712 |
| results of superior vena cava resection for lung cancer. analysis of prognostic factors. | the benefits of superior vena cava (svc) resection for lung cancer remain controversial. data obtained in four international centers were analyzed in order to identify prognostic factors and thus guide in future patient selection. | 2004 | 15140547 |
| metabolic effects of dietary lactose in adult female rats. | as an outgrowth of our interest in the potential toxicity of dietary galactose, we investigated the metabolic effects of high lactose diets in long-evans female rats. seventy-five long-evans female rats (25-day-old) were randomized to receive one of 3 diets for 7 months: glucose diet (con); low lactose diet (10.5%, lld); or a high lactose diet (41.9%, hld). necropsy was performed seven months after randomization. hld animals had significantly lower body weights than controls (p < 0.01). these an ... | 2003 | 15141440 |
| effect of dietary soy on serum thyroid hormone concentrations in healthy adult cats. | to compare effects of short-term administration of a soy diet with those of a soy-free diet on serum thyroid hormone concentrations in healthy adult cats. | 2004 | 15141877 |
| thyroxine plus low-dose, slow-release triiodothyronine replacement in hypothyroidism: proof of principle. | studies in hypothyroid rats show that, when infused with a combination of thyroxine (t4) plus triiodothyronine (t3) to normalize thyrotropin (tsh), euthyroidism in all organs is only ensured when t(4) and t(3) are administered in a ratio as normally secreted by the rat thyroid. as substitution with t(4)-only results in an abnormal serum t(4)/t(3) ratio, it is also possible that in humans, euthyroidism does not exist at the tissue level in many organs, considering that iodothyronine metabolism in ... | 2004 | 15142360 |
| descriptive epidemiology of thyroid carcinoma in carinthia, austria: 1984-2001. histopathologic features and tumor classification of 734 cases under elevated general iodination of table salt since 1990: population-based age-stratified analysis on thyroid carcinoma incidence. | the aim of the study was to investigate the current thyroid carcinoma (tc) incidence in carinthia, a former iodine-deficient, goiter-endemic region in austria with approximately 550,000 inhabitants from 1984 to 2001. using age-cohort analysis we analyzed the tc incidence under the impact of two regional risk factors: the contamination of austrian soils by the radioactive fallout in from chernobyl 1986 and the increased iodination of table salt in a general program of goiter prophylaxis begun in ... | 2004 | 15142361 |
| [relationship of matrilysin with invasion, metastasis, and prognosis in laryngeal cancer]. | matrilysin (matrix metalloproteinases-7, mmp-7) might play an important role in invasion and metastasis of tumor and is thought to be related to prognosis of many kinds of carcinomas. this study was designed to investigate the significance of mmp-7 expression in the invasion and metastasis of laryngeal cancer and the relationship of mmp-7 expression with the prognosis of laryngeal cancer. | 2004 | 15142461 |
| spontaneous k-complexes in behaving rats. | the k-complex (kc) is an electrographic rhythmic pattern present in human and cat sleep eeg. in long-term multisite videoeeg recordings in behaving sprague-dawley (sd) rats, well-defined spontaneous kcs were observed during sleep. sprague-dawley rats were implanted with multiple electrodes bilaterally along the antero-posterior axes at the locations f1, f2, f7, f8, t3, t4, p3, p4, all against a ground reference placed in the midline above the cerebellum. multiple, closely spaced cortical electro ... | 2004 | 15143624 |
| mixed mold mycotoxicosis: immunological changes in humans following exposure in water-damaged buildings. | the study described was part of a larger multicenter investigation of patients with multiple health complaints attributable to confirmed exposure to mixed-molds infestation in water-damaged buildings. the authors present data on symptoms; clinical chemistries; abnormalities in pulmonary function; alterations in t, b, and natural killer (nk) cells; the presence of autoantibodies (i.e., antinuclear autoantibodies [ana], autoantibodies against smooth muscle [asm], and autoantibodies against central ... | 2003 | 15143854 |
| modification of lymph node dissection for colon cancer by clinical diagnosis. | less invasive procedures for colon cancer that are available now increase the needs of modified lymph node dissection. the aim of the present study is to establish oncologically safe criteria for modifying lymph node dissection for colon cancer by clinical diagnosis. | 2004 | 15143901 |
| new proposal for t classification of gingival carcinomas arising in the maxilla. | when the current t classification of the uicc (1987 and 1997) is used to stage carcinomas arising the upper alveolus and gingival and hard palate, most cases are classified as t4 because of their anatomic characteristics, similar to carcinomas arising in the lower alveolus and gingiva. this study compared the following two methods for classifying the t stage of maxillary carcinomas: (1) the original t classification criteria proposed by the uicc (1987 and 1997), and (2) a new t classification cr ... | 2004 | 15145036 |
| esophagocardiac convergence onto thoracic spinal neurons: comparison of cervical and thoracic esophagus. | the aim of this study was to characterize thoracic spinal neurons receiving convergent inputs from the esophagus, heart and somatic receptive fields. extracellular potentials of single t3-t4 spinal neurons were recorded in pentobarbital anesthetized male rats. thoracic and cervical esophageal distensions (ted, ced) were produced by water inflation of a latex balloon. a catheter was placed in the pericardial sac to administer bradykinin or a mixture of algogenic chemicals. 96/311 (31%) neurons re ... | 2004 | 15145756 |
| protein displacement by an assembly of helicase molecules aligned along single-stranded dna. | helicases are molecular motors that unwind double-stranded dna or rna. in addition to unwinding nucleic acids, an important function of these enzymes seems to be the disruption of protein-nucleic acid interactions. bacteriophage t4 dda helicase can displace proteins bound to dna, including streptavidin bound to biotinylated oligonucleotides. we investigated the mechanism of streptavidin displacement by varying the length of the oligonucleotide substrate. we found that a monomeric form of dda cat ... | 2004 | 15146172 |
| changes in some hormones by low doses of di (2-ethyl hexyl) phthalate (dehp), a commonly used plasticizer in pvc blood storage bags & medical tubing. | di (2-ethyl hexyl) phthalate (dehp), a plasticizer commonly used in pvc blood storage bags leaches out in significant amounts into blood during storage. in view of many reports on the toxicity of this compound, it was considered necessary to investigate the effect of dehp at the low level solubilized in blood on some important hormones in rats and in human blood stored in dehp plasticized blood bags. | 2004 | 15147118 |
| amelioration by active hexose correlated compound of endocrine disturbances induced by oxidative stress in the rat. | active hexose correlated compound (ahcc), an extract derived from fungi of basidiomycetes family, has been found to be a potent antioxidant. since the secretion of some hormones can be affected by reactive oxygen species, the objective of this study was to examine how ferric nitrilotriacetate (fenta), which generates hydroxyl radicals in vivo, modulates the hormone secretion and the effects of ahcc. | 2004 | 15147233 |
| effectiveness of rna interference in transgenic plants. | rna interference (rnai) can be used to study gene function by effecting degradation of the targeted transcript. however, the effectiveness of transgene-induced rnai among multiple target genes has not been compared systematically. to this end, we developed a relative quantitative rt-pcr protocol that allows use of a single internal standard over a wide range of target gene expression levels. using this method in an analysis of transgenic arabidopsis thaliana rnai lines targeting 25 different end ... | 2004 | 15147899 |