| selection of reference mirnas for relative quantification in buffalo (bubalus bubalis) blastocysts produced by hand-made cloning and in vitro fertilization. | very low birth rate and a high incidence of abnormalities in offspring born from cloned embryos, which have limited the application of cloning technology on a wide scale, are believed to be because of incomplete or aberrant nuclear reprogramming. micrornas (mirnas) are involved in regulating a wide range of biological processes including reprogramming and embryonic development. selection of suitable reference mirnas is critical for normalization of data for accurate relative quantification of mi ... | 2019 | 31199674 |
| comparative analysis of buffalo (bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by scnt. | somatic cell nuclear transfer (scnt), using transgenic donor cells, is a highly efficient method for producing transgenic embryos. we compared the developmental competence, quality and gene expression of transgenic embryos produced by hand-made cloning from buffalo fetal fibroblasts (bffs) containing human insulin gene, with non-transgenic embryos produced from bffs (controls). the expression vector (pacisubc), constructed by inserting human insulin gene between dna fragments containing mammary ... | 2019 | 31195358 |
| an investigation of the concurrency of anti-neospora antibody and parasitemia in water buffalo (bubalus bubalis) in northwest of iran. | neospora caninum is an obligate intracellular parasite causing abortion and reproductive failure in ruminants. here, the seroprevalence of neospora dna and anti-neospora antibodies and the correlation between the dna and the antibody using polymerase chain reaction (pcr) and a new developed whole cell-based enzyme-linked immunosorbent assay (elisa) in water buffalo (bubalus bubalis) were investigated. to determine the level of anti-neospora antibody, 83 serum samples were collected from buffaloe ... | 2019 | 31183020 |
| comparative molecular characterization of forkhead box protein 3 (foxp3) gene of swamp-type (bubalus carabanensis) and riverine-type (bubalus bubalis) water buffaloes. | foxp3 is a forkhead family member that plays an important role in the development and function of a type of cd4 + t cell called t regulatory cells. molecular characterization of foxp3 gene in swamp- and riverine-type water buffaloes was conducted to determine its homology and compare it to the foxp3 gene of other animal species (cattle, goat, sheep, horse, pig, cat, and dog), determine its unique characteristics in water buffaloes, and provide a reference for future studies to analyze its immuno ... | 2019 | 31174683 |
| histology, histochemistry and ultrastructure of the nasopharyngeal tonsil of the buffalo (bubalus bubalis). | the light microscopic appearance and ultrastructure of the nasopharyngeal tonsil (tonsilla pharyngea), collected from 12 adult buffaloes of local mixed breed, were explored for the distribution of different types of epithelia, lymphoid tissue and high endothelial venules. the tonsillar mucosa was lined by pseudostratified columnar ciliated epithelium having goblet cells. the respiratory epithelium associated with the underlying lymphoid tissue formed the lymphoepithelium. the epithelium was furt ... | 2019 | 31173394 |
| addition of pomegranate juice (punica granatum) in tris-based extender improves post-thaw quality, motion dynamics and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. | the aim of the present study was to determine the protective effects of pomegranate juice in tris-based extender on semen parameters, computer-assisted sperm analysis (casa) motion characteristics and field fertility of post-thawed nili ravi buffalo (bubalus bubalis) bull spermatozoa. two consecutive ejaculates/collection from each of the five adult nili ravi buffalo bulls were collected with artificial vagina at 42°c for a period of 7 weeks, diluted in extender containing different concentratio ... | 2019 | 31134645 |
| traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases. | ruminant feed containing animal byproduct proteins (abps) is prohibited in many countries due to its risk of transmitting prion diseases (pd). in most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. | 2019 | 31131007 |
| synchronization with controlled internal drug release (cidr) and prostaglandin f2α (pgf2α) influences oxidant/antioxidant biomarkers and mineral profile in summer-stressed anoestrous buffalo (bubalus bubalis). | during the summer season, high ambient temperature in tropical and subtropical countries exposes buffaloes to oxidative stress that have antigonadotropic and antisteroidogenic effects. uses of hormonal therapy can improve the state of ovarian inactivity caused by heat stress and cause anoestrous buffaloes to come into oestrus and successfully achieve pregnancy. therefore, this study was designed to detect the role of oxidative stress in production of the anoestrous state in summer heat stressed ... | 2019 | 31129479 |
| stimulating effects of quercetin (que) in tris citric acid extender on post thaw quality and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa. | aim of this study was to evaluate the stimulating effects of quercetin (que) on post thaw quality and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa. que is a well-known flavonoid having antioxidant properties to scavenge free radicals. semen was collected from three buffalo bull through artificial vagina (42 °c) and were evaluated initially for volume, motility, concentration, followed by dilution in five extenders (n = 5 aliquots, control, c = no que; treatment 1, t1 = 50 μm q ... | 2019 | 31112913 |
| blood lymphocyte subpopulations in healthy water buffaloes (bubalus bubalis, mediterranean lineage): reference intervals and influence of age and reproductive history. | there is an increasing interest toward infectious diseases and mechanisms of immune response of water buffaloes, mainly because of the growing economic impact of this species and of its high-quality milk. however, little is known about the immune system of these animals in physiological conditions. recently, a wide number of antibodies cross reacting with buffalo antigens has been validated for use in flow cytometry (fc), allowing detailed characterization of the lymphocytic population in this s ... | 2019 | 31084895 |
| pattern of cd14, cd16, cd163 and cd172a expression on water buffalo (bubalus bubalis) leukocytes. | previous studies on the immune system of water buffalo (bubalus bubalis) using cross-reactive monoclonal antibodies (mabs) revealed significant similarities and differences to the bovine immune system. herein, we extend these studies and document the pattern of expression of cd14, cd16, cd163 and cd172a on buffalo leukocytes using a set of cross-reactive mabs that are known to recognize conserved epitopes within orthologous molecules in cattle, sheep and goats. buffalo leukocytes were isolated a ... | 2019 | 31084887 |
| is buffalo enamel a suitable substrate for bond strength tests? | the aim of this study was to compare the bonding strength of dental materials in buffalo (bubalus bubalis var. kerebau), bovine, and human enamel and the susceptibility of these substrates in acid etching. | 2019 | 31040720 |
| molecular evidence of the reservoir competence of water buffalo (bubalus bubalis) for anaplasma marginale in cuba. | water buffalo (bubalus bubalis) is a potential reservoir for anaplasma marginale in livestock ecosystems of tropical countries. however, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. in the present study, the reservoir competence of water buffalo for a. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of a. marginale strains from cattle and buffalo. eight gr ... | 2018 | 31014871 |
| serological investigation of h9n2 avian influenza virus in slaughtered water buffaloes (bubalus bubalis) in khuzestan, iran. | the aim of the present was to evaluate the prevalence of h9n2-specific antibodies among water buffaloes (bubalus bubalis). to this end, blood samples were obtained from 80 randomly selected water buffaloes, 40 cases of which were obtained in the winter months, and 40 cases were sampled in the spring months. the presence of h9n2-specific antibody was determined by hemagglutination inhibition method. the antibody was diagnosed in 14 buffaloes (i.e., 10 males and 4 females). there were no significa ... | 2019 | 31013009 |
| the first report of hydatid disease (echinococcus granulosus) in an australian water buffalo (bubalus bubalis). | a three year old female water buffalo was slaughtered for human consumption on a dairy buffalo farm in eastern new south wales, australia. gross examination of the offal revealed four small, superficial hydatid cysts in the liver and two larger superficial cysts in one lung. all organs were sliced and no other cysts were found. histology and pcr confirmed the cysts to be cysts of echinococcus granulosus senso stricto. none of the cysts contained protoscoleces. the source ofinfection is equivocal ... | 2019 | 30976509 |
| validation of an inhibition enzyme-linked immunosorbent assay system for the diagnosis of toxoplasma gondii infection in buffaloes (bubalus bubalis). | toxoplasmosis is one of the most widespread zoonoses in the world, due to the existence of a wide variety of toxoplasma gondii hosts, which include several domestic animal species. in cuba, there is sustained production of the bubalus species, which is highly adaptable and disease resistant, although it has been identified as a reservoir for a range of aetiological agents. several countries have reported buffaloes as the intermediate host of t. gondii, noting the need to carry out epidemiologica ... | 2018 | 30964470 |
| draft genome of the river water buffalo. | water buffalo (bubalus bubalis), a large-sized member of the bovidae family, is considered as an important livestock species throughout southeast asia. in order to better understand the molecular basis of buffalo improvement and breeding, we sequenced and assembled the genome (2n=50) of a river buffalo species bubalus bubalis from bangladesh. its genome size is 2.77 gb, with a contig n50 of 25 kb and the scaffold n50 of 6.9 mbp. based on the assembled genome, we annotated 24,613 genes for future ... | 2019 | 30962899 |
| effect of dietary supplementation of palm kernel cake on ovarian and hepatic function in buffalo (bubalus bubalis). | to determine the optimal inclusion amount of palm kernel cake (pkc) in a buffalo diet, in the present study there was evaluation of the ovarian activity, metabolism and hepatic function of females that were treated to synchronize the time of ovulation. twenty-four estrous-cyclic and non-lactating murrah buffalo with a mean age of 5.7 years were supplemented with 0%, 0.25%, 0.5% and 1% of their body weight (bw) with pkc. animals were subjected to the ovsynch protocol (beginning of protocol = d0). ... | 2019 | 30935774 |
| prevalence, risk factors, and spatial distribution of fasciola in carabao and intermediate host in baybay, leyte, philippines. | fasciolosis remains an important parasitic disease of ruminants in southeast asia. we investigated the prevalence and spatial distribution of fasciola spp. in carabao (bubalus bubalis) and snail intermediate hosts (ihs) in baybay, leyte, philippines. using one-stage cluster sampling, we examined 335 fecal samples of carabaos from 138 herds for fasciola spp. eggs, and in parallel collected ih snail data in the area. risk factor analyses were performed and gis maps were created for fasciola spp. p ... | 2019 | 30929938 |
| identifying hub genes for heat tolerance in water buffalo (bubalus bubalis) using transcriptome data. | heat stress has a detrimental effect on the physiological and production performance of buffaloes. elucidating the underlying mechanisms of heat stress is challenging, therefore identifying candidate genes is urgent and necessary. we evaluated the response of buffaloes (n = 30) to heat stress using the physiological parameters, elisa indexes, and hematological parameters. we then performed mrna and microrna (mirna) expression profiles analysis between heat tolerant (ht, n = 4) and non-heat toler ... | 2019 | 30918514 |
| karyotypic and mtdna based characterization of malaysian water buffalo. | in malaysia, the domestic water buffaloes (bubalus bubalis) are classified into the swamp and the murrah buffaloes. identification of these buffaloes is usually made via their phenotypic appearances. this study characterizes the subspecies of water buffaloes using karyotype, molecular and phylogenetic analyses. blood of 105 buffaloes, phenotypically identified as swamp, murrah and crossbred buffaloes were cultured, terminated and harvested using conventional karyotype protocol to determine the n ... | 2019 | 30909863 |
| effect of cumulus cells of cumulus-oocyte complexes on in vitro maturation, embryonic developmental and expression pattern of apoptotic genes after in vitro fertilization in water buffalo (bubalus bubalis). | in the present study, the potential of different grades of cumulus-oocyte complexes (cocs) for in vitro maturation (ivm) and embryonic development was assessed. further, the association of the expression pattern of anti-apoptotic mcl-1 and pro-apoptotic bax genes in embryonic development was analyzed. abattoir derived oocytes were graded into grade a and b based on surrounding cumulus rings. out of 1050 ovaries, a total number of 770 and 1360, were of grade a and b cocs, respectively, were aspir ... | 2020 | 30905313 |
| transcriptional regulation of thrombospondins and its functional validation through crispr/cas9 mediated gene editing in corpus luteum of water buffalo (bubalus bubalis). | thrombospondins (tsps) are large multi-modular proteins, identified as natural angiogenesis inhibitors that exert their activity by binding to cd36 and cd47 receptors. the anti-angiogenic effect of tsps in luteal regression of water buffalo has not been addressed. the present study characterized the expression pattern and localization of tsps and their receptors in ovarian corpus luteum during different stages of development in buffalo. this study also elucidated the effect of exogenous thrombos ... | 2019 | 30897320 |
| brain-derived neurotrophic factor (bdnf) is expressed in buffalo (bubalus bubalis) ovarian follicles and promotes oocyte maturation and early embryonic development. | brain-derived neurotrophic factor (bdnf) has been discovered and characterized for several decades, yet its expression pattern in non-neuronal tissues like ovary and potential mechanism during oocyte maturation are still poorly understood. thus the present study was devised to determine the expression pattern and mechanism of bdnf during buffalo oocyte maturation. the results revealed that bdnf was presented at different stages of buffalo ovarian follicles as well as during oocyte maturation and ... | 2019 | 30877846 |
| evidence of neospora caninum infection in buffaloes (bubalus bubalis) from northwestern romania. | neospora caninum is a heteroxenous parasitic apicomplexan protozoan, able to infect a variety of domestic and wild animals, mainly associated with reproductive disorders and abortions in susceptible species. considering the importance of romania in the european buffalo industry and the severe economic losses caused by n. caninum infection in livestock, the aim of the present study was to assess the occurrence of neosporosis in buffaloes raised in household and farming systems in northwestern rom ... | 2019 | 30877438 |
| intestinal anti-inflammatory effect of a peptide derived from gastrointestinal digestion of buffalo (bubalus bubalis) mozzarella cheese. | under physiological conditions, the small intestine represents a barrier against harmful antigens and pathogens. maintaining of the intestinal barrier depends largely on cell⁻cell interactions (adherent-junctions) and cell⁻matrix interactions (tight-junctions). inflammatory bowel disease is characterized by chronic inflammation, which induces a destructuring of the architecture junctional epithelial proteins with consequent rupture of the intestinal barrier. recently, a peptide identified by bub ... | 2019 | 30871183 |
| expression and functional role of kisspeptin and its receptor in the cyclic corpus luteum of buffalo (bubalus bubalis). | | 2019 | 30870709 |
| effects of follicle-stimulating hormone followed by gonadotropin-releasing hormone on embryo production by ovum pick-up and in vitro fertilization in the river buffalo (bubalus bubalis). | in this study, we examined the effects of superstimulation using follicle-stimulating hormone (fsh) followed by gonadotropin-releasing hormone (gnrh) on buffalo embryo production by ultrasound-guided ovum pick-up (opu) and in vitro fertilization (ivf). nine murrah buffaloes were subjected to opu-ivf without superstimulation (control). the morphologies of the oocytes collected were evaluated, and oocytes were then submitted to in vitro maturation (ivm). two days after opu, same nine buffaloes wer ... | 2019 | 30854764 |
| twinning rate in buffaloes: a case report. | the information of twin pregnancy frequencies in buffaloes is scarce in the literature. evidence even indicates that twin pregnancies are not carried to term, but it does not seem to be the case. in this case report, the event of twin pregnancy in buffaloes is reported as well as its frequency. twin birth is the presence of two or more embryos in a single gestation. multiple births are uncommon in buffaloes since the species is uniparous. we studied a population of murrah buffaloes in which slig ... | 2019 | 30821009 |
| the role of 5-aza-2'-deoxycytidine on methylation status of xist gene in different genders of buffalo (bubalus bubalis) bone marrow mesenchymal stem cells. | previous studies have demonstrated that proper concentration of 5-aza-2'-deoxycytidine (5-aza-cdr) treatment was advantageous to decrease dna methylation level, but the relationships between 5-aza-cdr treatment and methylation status of imprinted genes are seldom detected. the aim of this study was to investigate the effect of low concentration 5-aza-cdr treatment on the methylation status of imprinted gene xist in different genders of buffalo bone marrow mesenchymal stem cells (bmscs). bmscs we ... | 2019 | 30785778 |
| evaluation of therapeutic potential of recombinant buffalo lactoferrin n-lobe expressed in e. coli. | lactoferrin (lf) is a multifunctional bi-lobate iron-binding glycoprotein belonging to transferrin family with a mass of approximately 80 kd. being ubiquitously present in almost all biological secretions, it performs important biological functions. one of the earliest and very well-documented functions of lf is the antibacterial effect against broad spectrum gram-negative and gram-positive bacteria. in this study, buffalo lf n-lobe cdna was amplified, cloned and expressed as a fusion protein in ... | 2020 | 30773109 |
| in vitro anthelmintic effect of biologically synthesized silver nanoparticles on liver amphistome, gigantocotyle explanatum. | in order to ensure global food security a rationale approach is required to control all those factors which directly or indirectly affect the food productivity. the neglected helminthic diseases alone are responsible for huge economic losses to the agrarian stakeholders. the problem is further compounded by the emerging drug resistance in flukes against the commonly used anthelmintics like triclabendazole. therefore, the search for alternatives including the nano-based approaches has become a ne ... | 2019 | 30769019 |
| a fast and reliable polymerase chain reaction method based on short interspersed nuclear elements detection for the discrimination of buffalo, cattle, goat, and sheep species in dairy products. | aim of present study was the set up of a fast and reliable protocol using species-specific markers for the quali-quantitative analysis of dna and the detection of ruminant biological components in dairy products. for this purpose, the promoter of the gene coding for the α-lactoalbumin (lalba) was chosen as possible candidate for the presence of short interspersed nuclear elements (sines). | 2019 | 30744372 |
| isolation, characterization and conjugated linoleic acid production potential of bifidobacterial isolates from ruminal fluid samples of murrah buffaloes. | in the present study, we investigated the potential of bifidobacterium spp., isolated from ruminal fluid samples from buffaloes (bubalus bubalis) for conjugated linoleic acid (cla) production. a total of 294 isolates were obtained from 86 ruminal fluid samples using bifidus selective medium (bsm) medium, and based on phospoketolase assay, 24 isolates were presumptively confirmed to be bifidobacterium species. further, the isolates were confirmed morphologically, biochemically and by pcr assays f ... | 2019 | 30738138 |
| computational detection and experimental validation of segmental duplications and associated copy number variations in water buffalo ( bubalus bubalis ). | duplicated sequences are an important source of gene evolution and structural variation within mammalian genomes. using a read depth approach based on next-generation sequencing, we performed a genome-wide analysis of segmental duplications (sds) and associated copy number variations (cnvs) in the water buffalo (bubalus bubalis). by aligning short reads of olimpia (the reference water buffalo) to the umd3.1 cattle genome, we identified 1,038 segmental duplications comprising 44.6 mb (equivalent ... | 2019 | 30734132 |
| validation of a noseband pressure sensor algorithm as a tool for evaluation of feeding behaviour in dairy mediterranean buffalo (bubalus bubalis). | this research communication addresses the goal of validating an algorithm to monitor natural occurrence of feeding behaviours in dairy mediterranean buffalo based on the output of a noseband pressure sensor (rumiwatch®, halter). several characteristics of the feeding behaviour were detected with a very high (ruminating boluses), high (chews per bolus) and moderate degree of correlation (chews per minute) with video analyses (gold standard). all of them were associated with a low mean difference ... | 2019 | 30729911 |
| cytogenetic identification of khuzestani water buffalo. | the domestic water buffalo (bubalus bubalis) is an economically important livestock species in many asian and mediterranean countries. two types of water buffalos are river and swamp buffaloes. genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = 50). khuzestan province is one of the important centers of buffalo farming in iran. since there was no cytogenetic information about khuzestani buffalo breed, the present study was performed to provi ... | 2018 | 30713615 |
| chemical capture of wild swamp buffalo (bubalus bubalis) in tropical northern australia using thiafentanil, etorphine and azaperone combinations. | studying wild animals in situ is fundamental to collecting baseline information, but generally they need to be immobilised for examination, sampling, marking and/or equipping with tracking apparatus. capturing wild animals is inherently risky and there is a need for immobilisation methods that are safe for both the animals and researchers. | 2019 | 30693492 |
| economic values and selection indices for production and reproduction traits of iranian buffaloes (bubalus bubalis). | iranian buffalo plays a critical role in supplying a portion of the income and the necessities of the rural population. the first step to design a breeding program is difinition of breeding goal (bg), a linear combination of breeding values for various traits and their economic values (ev). the current study was aimed at determining evs for important traits of iranian buffaloes, namely milk yield (my), milk fat (mf), age at the first calving (afc), and calving interval (ci), as well as at estima ... | 2019 | 30684223 |
| chromosome-level assembly of the water buffalo genome surpasses human and goat genomes in sequence contiguity. | rapid innovation in sequencing technologies and improvement in assembly algorithms have enabled the creation of highly contiguous mammalian genomes. here we report a chromosome-level assembly of the water buffalo (bubalus bubalis) genome using single-molecule sequencing and chromatin conformation capture data. pacbio sequel reads, with a mean length of 11.5 kb, helped to resolve repetitive elements and generate sequence contiguity. all five b. bubalis sub-metacentric chromosomes were correctly s ... | 2019 | 30651564 |
| first study of sperm mediated gene transfer in egyptian river buffalo. | the present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pegfp-n1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (smgt). the tested conditions were plasmid dna concentration, sperm concentration, transfecting agent concentration: dimethyle sulphoxide (dmso) and time of transfection. the study proved that the best conditions for producing transgenic emb ... | 2017 | 30647689 |
| differential expression of immune response genes associated with subclinical mastitis in dairy buffaloes. | buffalo milk production has become of significant importance on the world scale, however, there are few studies involving biotechnological tools specifically for buffalo. to verify the effects caused by subclinical mastitis on the components of milk and to study the innate immune system in the udder of dairy buffaloes with subclinical mastitis, we evaluated the levels of expression of the lactoferrin (ltf), tumor necrosis factor alpha (tnf-α), interleukin-1 beta (il-1β), interleukin-8 (il-8), an ... | 2019 | 30621802 |
| optimization of serum-free culture conditions for propagation of putative buffalo (bubalus bubalis) spermatogonial stem cells. | spermatogonial stem cells (sscs) self-renew and produce a large number of differentiated germ cells to maintain normal spermatogenesis. however, the growth factors crucial for ssc self-renewal and the mechanism underlying this process remain unclear. in the present study, a serum-free culture media was used to evaluate the effect of several growth factors on the expression of some ssc markers and self-renewal related genes. the putative sscs were cultured on buffalo sertoli cell feeder layer in ... | 2019 | 30601028 |
| characterization of drug resistance-associated tevat1 gene of trypanosoma evansi from philippine water buffaloes (bubalus bubalis) | this study detected and characterized the tevat1 gene of trypanosoma evansi isolates from philippine water buffaloes (bubalus bubalis). a total of 68 blood samples from philippine water buffaloes were subjected to dna extraction and pcr assay was performed using rotat 1.2 gene to detect t. evansi. those samples positive for t. evansi subsequently underwent another pcr assay to detect the presence of tevat1 gene. trypanosoma evansi was detected in 26.47% (18/68) blood samples in which distributed ... | 2019 | 32191985 |
| supplementation of glial cell line-derived neurotrophic factor, fibroblast growth factor 2, and epidermal growth factor promotes self-renewal of putative buffalo (bubalus bubalis) spermatogonial stem cells by upregulating the expression of mir-20b, mir-21, and mir-106a. | in this study, we investigated the effect of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor (fgf) 2, and epidermal growth factor (egf) on the expression of some self-renewal-related micrornas (mirs) in putative buffalo spermatogonial stem cells (sscs). the sscs were cultured on a buffalo sertoli cell feeder layer, colony formation was observed between 7 and 10 days. the ssc colonies expressed markers specific for undifferentiated type a spermatogonia and pluripotenc ... | 2019 | 30589561 |
| retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (bubalus bubalis) oocytes. | retinoic acid, vitamin a metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. the objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisra) on the quality and maturation rate of buffalo oocytes. cumulus-oocyte complexes (cocs, n = 460) were collected from ovaries of slaughtered buffalos. varying concentrations of 9-cisra (0, 5, 50, and 200 nm) were added to the maturation ... | 2018 | 30564610 |
| ostrich egg yolk improves post thaw quality and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. | egg yolk containing a higher ratio of phospholipids and cholesterol may have better cryoprotective effect to buffalo spermatozoa during cryopreservation. our objectives were to ascertain the comparison of ostrich and chicken egg yolk in semen extender on post thaw quality, motion dynamics and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 45) from five bulls were collected once a week for a period of nine weeks and diluted in triladyl® extender havi ... | 2019 | 30551020 |
| presence of cadmium residues in muscle, liver and kidney of bubalus bubalis and histological evidence. | cadmium (cd) concentrations were evaluated in the samples of kidney, liver and muscle of sixty-six buffaloes regularly slaughtered. forty were raised in campania, in the territory between the province of naples and caserta and twenty-six were bred in apulia, in the province of bari. two aliquots were prepared for the renal and hepatic samples: one intended for the chemical analysis and the other one intended for histological investigations. muscle samples were the subject of purely chemical inve ... | 2018 | 30538963 |
| prenatal development of retina in buffalo (bubalus bubalis). | the development of retina in indian buffalo (bubalus bubalis) has not been reported previously. the aim of the present study was therefore to report the major landmarks and the time course in the development of retina. serial histological sections of indian buffalo embryos and foetuses were used as group1 (<20.0 cm cvrl), group2 (>20.0 but <40.0 cm cvrl) and group3 (>40.0 cm cvrl). age estimation was made on the basis of crown vertebral-rump length (cvrl), which ranged between 36 and 286 days (1 ... | 2019 | 30536596 |
| treatment of buffalo (bubalus bubalis) scnt embryos with microrna-21 mimic improves their quality and alters gene expression but does not affect their developmental competence. | application of cloning technology on a wide scale is severely limited by the very low live birth rate obtained with cloned embryos. embryo quality is an important factor which affects the conception and live birth rate of cloned embryos. microrna-21 (mir-21) has been implicated in the regulation of apoptosis and the expression level of several important genes which control apoptosis. we examined the effects of treatment of reconstructed buffalo embryos, produced by hand-made cloning, with mir-21 ... | 2019 | 30508790 |
| scrotal infrared digital thermography predicts effects of thermal stress on buffalo (bubalus bubalis) semen. | the objective was to use scrotal infrared digital thermography to evaluate effects of thermal stress on semen quality of murrah buffalo (bubalus bubalis) breeding bulls. ejaculates from 109 murrah bulls maintained at three semen stations were evaluated for: ejaculate volume, sperm concentration (scon), initial motility (im), percent live sperm (live), acrosome integrity of fresh semen (aifs), plasma membrane integrity of fresh semen (pmifs), head abnormalities of fresh semen (hafs), midpiece abn ... | 2018 | 30509667 |
| regulation of steroidogenic function of luteal cells by thrombospondin and insulin in water buffalo (bubalus bubalis). | the present study examined the effect of exogenous thrombospondin 1 (tsp1) on the steroidogenic function of luteal cells cultured invitro. furthermore, the transcriptional interaction of insulin with tsp1 and its receptor, cluster of differentiation 36 (cd36) were also investigated. at the highest dose (500ngml-1) tsp1 significantly downregulated the expression of the angiogenic marker von willebrand factor (vwf) and progesterone production in cultured luteal cells. moreover, the simultaneous up ... | 2019 | 30509339 |
| genomewide identification and annotation of snps in bubalus bubalis. | the present study was carried out to identify and annotate the genome wide snps in murrah buffalo genome. a total of 21.2 million raw reads from 4 pooled female murrah buffalo samples were obtained using restriction enzyme digestion followed by sequencing with illumina hiseq 2000. after quality filtration, the reads were aligned to murrah buffalo genome (icar-nbagr) and water buffalo genome (umd_caspur_wb_2.0) which resulted in 99.37% and 99.67% of the reads aligning, respectively. a total of 13 ... | 2019 | 30476556 |
| reactive oxygen metabolites in alpha-herpesvirus-seropositive mediterranean buffaloes (bubalus bubalis): a preliminary study. | in the present study on bubalus bubalis of the campania region (italy) the serum levels of derivatives of reactive oxygen metabolites (d-roms), anti-rom and oxidative stress index (osi) were evaluated. these data were then related to the seropositive status of the animals against alpha-herpesviruses, precisely bubaline herpesvirus 1 (buhv-1) and bovine herpesvirus 1 (bohv-1). clinically healthy mediterranean buffaloes were selected for this study. the serum samples of these animals were taken, a ... | 2018 | 30468352 |
| prevalence and associated factors with neospora caninum infection in female water buffaloes (bubalus bubalis) from pernambuco, brazil. | neosporosis is one of the major parasitic diseases that causes reproductive problems, making it a limitation for optimal efficiency in livestock. the aim of the present study was to conduct a seroepidemiological survey for bubaline neosporosis and to analyse associated factors associated with n. caninum infection in female water buffaloes in the state of pernambuco, northeast brazil. the prevalence of n. caninum infection in the bubaline herds examined was 35.4% (122/345; 95% ci: 30.4%-40.7%), a ... | 2019 | 30427520 |
| the single nucleotide polymorphism g.133a>c in the stearoyl coa desaturase gene (scd) promoter affects gene expression and quali-quantitative properties of river buffalo milk. | the stearoyl-coa desaturase (scd) gene has been investigated in depth in ruminants because of its effect on milk fat composition. in river buffalo, the single nucleotide polymorphism (snp) g.133a>c in the gene promoter has been associated with milk quality and yield. however, the biological reason for such effects remains unexplored. in this study, we combined mrna profile analysis, an electromobility shift assay, and quantitative pcr to elucidate the role of this snp on gene transcription and i ... | 2019 | 30391181 |
| occurrence of amblyomma mixtum on the water buffalo (bubalus bubalis) in mexico. | the water buffalo (bubalus bubalis) is an asian species of bovine that was introduced in mexico in 1992 as an alternative for milk and meat production. to date, no surveys have been conducted to identify ticks acquired by water buffaloes since their arrival in the country. here we report, for the first time, the presence of amblyomma mixtum, a neotropical tick in the amblyomma cajennense complex, found on water buffaloes from mexico and discuss its possible implications in veterinary public heal ... | 2018 | 30386724 |
| polymorphism of melatonin receptor (mtnr1a) gene and its association with seasonal reproduction in water buffalo (bubalus bubalis). | the water buffalo have a seasonal pattern of reproduction with decreased sexual activity during the longer photoperiod. the present study was designed to identify a single nucleotide polymorphism (snp) in the mtnr1 a gene and its association with seasonal reproduction and reproductive characteristics in murrah buffalo cows. the 812 bp fragment encompassing exon ii of the mtnr1 a gene was amplified from genomic dna of 190 pluriparous murrah buffalo cows. amplified pcr products from 12 samples wer ... | 2018 | 30384994 |
| propensity in low-grade oocytes for delayed germinal vesicle breakdown compromises the developmental ability of sub-optimal grade bubalus bubalis oocytes. | summarymaturing oocytes have diverse developmental potential and good quality oocytes exhibit a better ability to attain physiological milestones in a time-dependent manner. this situation necessitates the confirmation of oocyte developmental status more precisely under an in vitro embryo production (ivep) regime. the aim of this study was to explain timely events in germinal vesicle breakdown (gvbd), an important milestone of oocyte nuclear maturation, to delineate the developmental capacity of ... | 2018 | 30289096 |
| selenium and copper interaction at supra-nutritional level affecting blood parameters including immune response against p. multocida antigen in murrah buffalo (bubalus bubalis) calves. | minerals play important role in the diet of an animal. bio-availability of minerals largely gets affected by absolute as well as the relative amount of each mineral present in the diet of an animal. copper and selenium are two such an essential elements affect utilization of each other in the gastrointestinal tract. the present study elucidates the utilization of copper and selenium at supra-nutritional levels (higher than nutritional requirements). male murrah buffalo (bubalus bubalis) calves ( ... | 2018 | 30262314 |
| ultrasonography as a diagnostic tool in egyptian buffaloes (bubalus bubalis) with traumatic pericarditis. | traumatic pericarditis (tp) remains a serious problem facing bovine producers; particularly in the developing countries; causing severe economic losses. this study was carried out on 47 buffaloes including; control buffaloes (n = 20) and buffaloes with tp (n = 27) at assiut governorate, egypt. all animals were subjected to thorough clinical examination, whole blood profiling, blood serum biochemical assays, and radiographic and ultrasonographic examinations. the recorded ultrasonographic finding ... | 2017 | 30255066 |
| normal magnetic resonance anatomy of the hind foot of egyptian buffalo (bubalus bubalis): a correlative low-field t1- and t2-weighted mri and sectional anatomy atlas. | buffaloes represent a major source of milk production, especially in developing countries including egypt. the buffalo foot is frequently involved in a large proportion of lameness cases. the relatively small size and complexity of its structures often render the radiographic evaluation of the foot challenging. magnetic resonance imaging (mri) is a noninvasive imaging technique that is regarded as both safe and accurate for assessment of the foot disorders in both man and animals. the purpose of ... | 2018 | 30221389 |
| expression profile of the csf3 and lpo genes in milk from buffalo (bubalus bubalis) with and without mastitis. | this study compared the expression profile of the candidate genes, csf3 and lpo, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. the granulocyte colony stimulating factor 3 (csf3) and lactoperoxidase (lpo) genes expression profiles were determined in 24 milk samples from buffaloes with (n = 12) and without (n = 12) mastitis, using the quantitative real-time pcr (qrt-pcr) technique. csf3 and ... | 2018 | 30217657 |
| endometrial transcript profile of progesterone-regulated genes during early pregnancy of water buffalo (bubalus bubalis). | progesterone (p4 ) plays a key role in the establishment and maintenance of pregnancy in most mammals. unravelling the expression of progesterone-regulated genes can expand the understanding of the embryonic mortality. accordingly, we studied the relative mrna expression of the p4 -regulated genes in the buffalo. uteri were collected from the abattoir and categorized into nonpregnant late luteal phase, stage i (28-38th days of gestation) and stage ii (48-56th days of gestation) of pregnancy (n = ... | 2019 | 30152910 |
| established method of chondroitin sulphate extraction from buffalo (bubalus bubalis) cartilages and its identification by ftir. | a study was conducted for extraction of chondroitin sulphate (cs) from buffalo tracheal, nasal and joint cartilages. cs was extracted from cartilages using 0.25% papain digestion, dialyzed, precipitated with 10% tca and finally lyophilized to dry powder. dimethylmethylene blue assay was performed to estimate the quantity of cs extracted. identification of extracted cs was performed with sds-page and fourier transforms infrared spectroscopy (ftir). sds-page analysis of extracted cs revealed simil ... | 2018 | 30150802 |
| recombinant interferon stimulated protein 15 (risg15) as a molecular marker for detection of early pregnancy in bubalus bubalis. | early and accurate diagnosis of pregnancy in animals is important for improving the reproductive management of livestock. the buffalo (bubalus bubalis) is the most important dairy animal in india, but there are reproductive problems resulting from extended calving interval and ovulation occurring in the absence of behavioral estrus. the lack of simple methods for early pregnancy diagnosis intensifies these problems. the present study, therefore, was conducted to ascertain the role of the interfe ... | 2018 | 30145042 |
| sperm functional attributes and oviduct explant binding capacity differs between bulls with different fertility ratings in the water buffalo (bubalus bubalis). | we report here the differences in sperm functional attributes and sperm-oviduct binding index in bulls with different field fertility ratings. cryopreserved spermatozoa from murrah buffalo bulls (n=9) with different fertility ratings were evaluated for membrane integrity, capacitation status, acrosome intactness and protein tyrosine phosphorylation status. frozen--thawed spermatozoa were incubated with oviduct explants for 1h under 5% co2, 38.5°c with 95% relative humidity and the number of sper ... | 2019 | 30135005 |
| an update: reproductive handmade cloning of water buffalo (bubalus bubalis). | the first birth of a cloned animal produced through the handmade cloning (hmc) technique was reported more than 15 years ago in cattle. this method of somatic cell nuclear transfer (scnt) has subsequently been evolving as a much simpler alternative to the classical micromanipulator-based scnt. several farm animal species such as cattle, buffalo, pigs, sheep, and goats have been successfully cloned using hmc. in buffalo, hmc technique is now well established, and several births of cloned calves h ... | 2018 | 30122268 |
| developmental competence of buffalo (bubalus bubalis) denuded oocytes cocultured with cumulus cells: protective role of cumulus cells. | the objectives of the present study were to evaluate the developmental competence of buffalo denuded oocytes (dos) cocultured with cumulus cells (ccs) during in vitro maturation, and to investigate the mechanisms by which ccs promote oocyte maturation and development. buffalo oocytes were matured in vitro for 24 h in three groups: (1) intact cumulus-oocyte complexes (cocs) (2) dos cocultured with ccs (doscc), and (3) dos cultured alone (dos). matured oocytes were used to determine the relative m ... | 2018 | 30092373 |
| epidural anesthesia in egyptian water buffalo (bubalus bubalis): a comparison of lidocaine, xylazine and a combination of lidocaine and xylazine. | to evaluate and compare the analgesic effects of a combination of lidocaine and xylazine to lidocaine or xylazine administered alone for epidural anesthesia in egyptian water buffalo (bubalus bubalis). | 2018 | 30078532 |
| effect of sex differences in donor foetal fibroblast on the early development and dna methylation status of buffalo (bubalus bubalis) nuclear transfer embryos. | low efficiency of somatic cell nuclear transfer (scnt) embryos is largely attributable to imperfect reprogramming of the donor nucleus. the differences in epigenetic reprogramming between female and male buffalo cloned embryos remain unclear. we explored the effects of donor cell sex differences on the development of scnt embryos. we and then compared the expression of dna methylation (5-methylcytosine-5mc and 5-hydroxymethylcytosine-5hmc) and the expression level of relevant genes, and histone ... | 2019 | 30051521 |
| characterization of naturally occurring, new and persistent subclinical foot-and-mouth disease virus infection in vaccinated asian buffalo in islamabad capital territory, pakistan. | the convalescent subclinical carrier state of foot-and-mouth disease virus (fmdv) infection has been thoroughly investigated; contrastingly, the subclinical form of new infections of vaccinated and naïve hosts is recognized, but poorly understood. to investigate the natural dynamics of subclinical fmdv infections, a prospective, 12-month, longitudinal study was conducted in vaccinated asian buffalo (bubalus bubalis) under natural conditions in pakistan, where fmdv is hyperendemic. oropharyngeal ... | 2018 | 30035376 |
| sources and levels of trace elements influence some blood parameters in murrah buffalo (bubalus bubalis) calves. | sources of supplemental minerals in the diet of animals are of important significance. bio-availability of organic sources is believed to be more in the body as compared to regularly used inorganic sources and hence environment-friendly due to reduced mineral excretion, which in turn reduces their requirements in the diet as well. twenty-four male murrah buffalo (bubalus bubalis) calves (about 18-20 months of age and 318.54 ± 8.85 kg body weight) were divided randomly into four groups of six ani ... | 2019 | 30014281 |
| evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (bubalus bubalis) bull spermatozoa. | egg yolk is used as a cryoprotectant in semen preservation. however, its composition varies according to the species which may influence its effectiveness during the freeze-thaw process. therefore, study was conducted to identify the optimum level of pigeon egg yolk (pey) in tris citric acid (tca) extender for freezability and in vivo fertility of buffalo semen. semen was collected at weekly intervals for a period of three weeks (replicates) from 6 nili ravi buffalo bulls (2 ejaculates/bull/repl ... | 2018 | 30006129 |
| the snp g.4667g>a at 3'-utr of ifng gene is associated with susceptibility to bovine tuberculosis in mediterranean water buffalo (bubalus bubalis). | | 2018 | 29999184 |
| molecular identification of buxtonella sulcata from associated-diarrhea in water buffaloes (bubalus bubalis) in the philippines | sixty suspected protozoan oocysts were demonstrated from 260 fecal samples collected from water buffaloes aged one month to seven years old with clinical signs of diarrhea in four provinces in the philippines after conventional methods of isolation, sporulation, morphological characteristics and kinyoun acid fast staining techniques. the recovered protozoan oocysts were subjected to molecular analysis. amplification of dna extractedfrom recovered eimeria oocysts using universal primers for the i ... | 2018 | 29983020 |
| cytoplasmic volume of recipient oocytes affects the nucleus reprogramming and the developmental competence of hmc buffalo (bubalus bubalis) embryos. | the present study was undertaken to examine the effects of cytoplasmic volume on nucleus reprogramming and developmental competence of buffalo handmade cloning (hmc) embryos. we found that both hmc embryos derived from ~150% cytoplasm or ~225% cytoplasm resulted in a higher blastocyst rate and total cell number of blastocyst in comparison with those from ~75% cytoplasm (25.4 ± 2.0, 27.9 ± 1.6% vs. 17.9 ± 3.1%; 150 ± 10, 169 ± 12 vs. 85 ± 6, p<0.05). meanwhile, the proportions of nuclear envelope ... | 2018 | 29925699 |
| comparison of tris egg yolk-based, triladyl® and optixell® extender on post-thaw quality, kinematics and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. | our objectives were to ascertain the comparison of tris egg yolk-based, triladyl® and optixell® extender on postthaw quality, casa parameters and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 35) from five bulls were diluted in tris egg yolk-based, triladyl® , optixell® extender and frozen in 0.50 ml french straws. postthaw sperm casa motility (%) was higher (p < 0.05) in optixell extender as compared to triladyl and tris egg yolk-based extender. a ... | 2018 | 29920732 |
| effects of an automatic milking system on milk yield and quality of mediterranean buffaloes. | a study was carried out to evaluate the effects of an automatic milking system (ams) on milk yield and composition of buffalo (mediterranean-type bubalus bubalis) cows. performed from january 2015 to december 2015 in an organic buffalo dairy farm equipped with both a traditional tandem milking parlor and an ams, the study involved 90 primiparous buffaloes randomly allotted to a tandem or ams group from 5 to 10 d of lactation onward. number of milkings per day and daily milk yield of each cow wer ... | 2018 | 29908804 |
| label-free proteome of water buffalo (bubalus bubalis) seminal plasma. | the study aimed to describe the bubalus bubalis seminal plasma proteome using a label-free shotgun udmse approach. a total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. in conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which establishes a ... | 2018 | 29888513 |
| prevalence and risk factors associated with ectoparasite infestation of buffaloes in an amazonian ecosystem. | the water buffalo (bubalus bubalis) is well adapted in some regions of the amazon. of all brazilian states, pará contains the largest number of this species, with 510,000 animals, approximately 38% of the brazilian buffaloes. despite the socioeconomic importance of bubaline farming in the northern region, little is known about the prevalence of ectoparasites that affect buffalo herds. this study aimed to identify the species of buffalo ectoparasites in the municipality of santarém, pará, and to ... | 2018 | 29866180 |
| investigation of the persistence of penicillin g and dihydrostreptomycin residues in milk of lactating buffaloes ( bubalus bubalis) using ultra-high-performance liquid chromatography and tandem mass spectrometry. | the purpose of this research was to evaluate the persistence of penicillin g and dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin g (200000 iu/ml) and dihydrostreptomycin sulfate (250 mg/ml) every 24 h for 3 days. milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. the analytical method has been validated according ... | 2018 | 29862808 |
| comparative diversity analysis of ruminal methanogens in murrah buffaloes (bubalus bubalis) in four states of north india. | we compared the community structure of methanogens in murrah breed of buffaloes of four states of north india using 16s rrna gene clone library method. the results revealed the dominance of methanogens related to methanobrevibacter in three states, while methanomicrobium-related methanogens were abundant in one state. | 2018 | 29859302 |
| bovine viral diarrhea virus subgenotype 1b in water buffalos (bubalus bubalis) from brazil. | serological studies have characterized the presence of the bovine viral diarrhea virus (bvdv) infection in water buffalo herds worldwide. however, the molecular characterization of bvdv strains circulating in this animal species is uncommon. the aim of this study was to identify young water buffalo with acute infection and characterize the subgenotype of the infecting wild-type bvdv strain. two dairy water buffalo herds from northeastern brazil were selected based on the results of virus neutral ... | 2018 | 29855763 |
| developmental and molecular responses of buffalo (bubalus bubalis) cumulus-oocyte complex matured in vitro under heat shock conditions. | summaryto investigate the effects of physiologically relevant heat shock during oocyte maturation, buffalo cumulus-oocyte complexes (cocs) were cultured at 38.5°c (control) or were exposed to 39.5°c (t1) or 40.5°c (t2) for the first 6 h of in vitro maturation (ivm), followed by 38.5°c through the next 18 h/ivm and early embryonic development up to the blastocyst stage. gene expression analysis was performed on selected target genes (hsf-1, hsf-2, hsp-70, hsp-90, bax, p53, sod1, cox1, mapk14) in ... | 2018 | 29785894 |
| reference 1d and 2d electrophoresis maps for potential disease related proteins in milk whey from lactating buffaloes and blood serum from buffalo calves (water buffalo, bubalus bubalis). | the aim of this study was to identify potential disease related proteins in milk whey of lactating buffaloes and blood serum of buffalo calves, in order to define a reference electrophoresis map for 1-de and 2-de. additionally, changes in some protein patterns from buffalo calves during salmonellosis and lactating buffaloes during mastitis are presented. milk samples were collected and distributed into groups: milk samples from healthy buffaloes (scc < 100.000 cells/ml, negative microbiology and ... | 2018 | 29734122 |
| m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (bubalus bubalis) embryos. | incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. in this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (cbha) on in vitro development of buffalo embryos produced by hand-made cloning. cloned embryos were treated with cbha (0, 5, 10, 20 or 50 μm) for 10 hr from the start of reconstruction till activation. at 10 μm, but not at other concentrations examined, cbha increased (p < ... | 2018 | 29733525 |
| efficient generation of transgenic buffalos (bubalus bubalis) by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein. | the possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (egfp) was explored in this study. when buffalo fetal fibroblasts (bffs) isolated from a male buffalo fetus were transfected with pegfp-n1 (egfp is driven by cmv and neo is driven by sv-40) by means of electroporation, lipofectamine-ltx and x-tremegene, the transfection efficiency of electroporation (35.5%) was higher than lipofectamine-ltx (11.7%) and x- ... | 2018 | 29725050 |
| re-evaluation of endogenous development of eimeria bareillyi gill, chhabra and lall, 1963 in water buffalo (bubalus bubalis). | water buffalo (bubalus bubalis) is important for the economy of asia, south america and parts of europe. coccidiosis is an important cause of neonatal mortality in livestock, including buffalo. of more than 12 species of eimeria in buffalo, eimeria bareillyi is the most pathogenic. there are uncertainties concerning its asexual and sexual development. during a previously reported outbreak of fatal enteritis associated with e. bareillyi in buffaloes in the netherlands, sections of small intestine ... | 2018 | 29692286 |
| domestic water buffaloes: access to surface water, disease prevalence and associated economic losses. | given the shortage and non-availability of freshwater in pakistan, wastewater is being used for bathing water buffaloes; however, this has a negative impact on animal welfare. although there is a vast literature on indirect linkages between wastewater and animal productivity, studies focusing on the direct impacts of water buffaloes bathing in wastewater on animal productivity and economic losses are rare. therefore, using 360 domestic water buffalo farms, this study examines the expenditure and ... | 2018 | 29685433 |
| testosterone levels and the genetic variation of sex hormone-binding globulin gene of bubalus bubalis, bulls in egypt. | testosterone is themain plasma androgen produced and secreted by male testis. to be bioactive, it binds to sex hormonebinding globulin (shbg), i.e. the major transporter protein of sex steroids in the blood of mammals and other vertebrate species.firstly, this study aimed to determine the levels of testosterone in different-age groups of buffalo bulls (bubalus bubalis) in egypt, and secondly to screen the genetic polymorphisms in their shbg gene and to investigatewhether these polymorphisms are ... | 2018 | 29666348 |
| antimicrobial peptides of buffalo and their role in host defenses. | antimicrobial peptides (amps) are highly conserved components of the innate immune system found among all classes of life. buffalo (bubalus bubalis), an important livestock for milk and meat production, is known to have a better resistance to many diseases as compared to cattle. they are found to express many amps such as defensins, cathelicidins, and hepcidin which play an important role in neutralizing the invading pathogens. buffalo amps exhibit broad-spectrum antimicrobial activity against b ... | 2018 | 29657403 |
| first molecular characterization and morphological aspects of sarcocystis fusiformis infecting water buffalo bubalus bubalis in egypt. | fresh muscle samples from water buffalo (bubalus bubalis) aged 2-15, from giza province, egypt; were examined for sarcocystis infection. macroscopic ovoid sarcocysts embedded in the muscle tissues of the examined buffaloes were detected; they measured 152-230 (210 ± 7) μm in length and 37-119 (95 ± 3) μm in width. the esophagus was the most infected organ followed by the diaphragm, and tongue, while the heart muscles were the least infected. the cyst cavity was compartmentalized by septa derived ... | 2018 | 29654684 |
| cloning of buffalo, a highly valued livestock species of south and southeast asia: any achievements? | buffalo (bubalus bubalis) is a major source of milk, meat, and draught power in many developing countries in asia. animal cloning holds a lot of potential for fast multiplication of elite buffaloes and conservation of their valuable germplasm. although the progress of buffalo cloning has been slow in comparison to cattle or pig, several breakthroughs were reported in buffalo cloning such as the production of cloned calves from somatic cells isolated from over one-decade old frozen-thawed semen o ... | 2018 | 29620444 |
| metabolic and hormonal adaptation in bubalus bubalis around calving and early lactation. | pregnancy and lactation are physiological periods that result in an increased metabolic demand that, if not satisfied, could provoke a threat to homeostasis. in this study changes in the values of non-esterified fatty acids (nefa) and β-hydroxybutyrate (bhb), glucose, insulin, thyroid hormones, milk composition and yield were investigated in buffaloes during the late pregnancy and early lactation. from a total of 50 buffaloes, blood samples were collected -7±5 days before expected calving; +7±5; ... | 2018 | 29617370 |
| blood gas and serum biochemical ris for healthy newborn murrah buffaloes (bubalus bubalis). | there is a lack of published work on ris for newborn buffaloes. establishing blood gas and serum biochemical ris for newborn buffaloes is important for monitoring health. | 2018 | 29575143 |
| remarkable genetic diversity detected at river buffalo prolactin receptor (prlr) gene and association studies with milk fatty acid composition. | prolactin is an anterior pituitary peptide hormone involved in many different endocrine activities and is essential for reproductive performance. this action is mediated by its receptor, the prolactin receptor, encoded by the prlr gene. in this study, we sequenced and characterized the mediterranean river buffalo prlr gene (from exon 3 to 10), and we found remarkable genetic diversity. in particular, we found 24 intronic polymorphisms and 13 exonic snps, seven of which were non-synonymous. furth ... | 2018 | 29569734 |
| new insights on water buffalo genomic diversity and post-domestication migration routes from medium density snp chip data. | the domestic water buffalo is native to the asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. the two types of water buffalo, i.e., river and swamp, display distinct morphological and behavioral traits, different karyotypes and also have different purposes and geographical distributions. river buffaloes from pakistan, iran, turkey, egypt, romania, bulgaria, italy, mozambique, brazil and colombia, and swamp buffaloes from china, thai ... | 2018 | 29552025 |
| a review on breeding and genetic strategies in iranian buffaloes (bubalus bubalis). | the aim of current study was to review breeding progress and update information on genetic strategies in iranian buffaloes. iranian buffalo is one of the vital domestic animals throughout north, north-west, south and south-west of iran with measurable characteristics both in milk and meat production. the species plays an important role in rural economy of the country due to its unique characteristics such as resistance to diseases and parasites, having long productive lifespan and showing higher ... | 2018 | 29524107 |
| isolation and characterization of buffalo (bubalus bubalis) amniotic mesenchymal stem cells derived from amnion from the first trimester pregnancy. | amniotic mesenchymal stem cells (amscs) from livestock are valuable resources for animal reproduction and veterinary therapeutic. the purpose of this study is to explore a suitable way to isolate and culture the buffalo amscs (bamscs), and to identify their biological characteristics. digestion with a combination of trypsin-edta and collagenase type i could obtain pure bamscs more effectively than trypsin-edta or collagenase type i alone. bamscs could proliferate steadily in vitro culture and ex ... | 2018 | 29515060 |