| pattern of white matter abnormalities at mr imaging: use of polymerase chain reaction testing of guthrie cards to link pattern with congenital cytomegalovirus infection. | to define a magnetic resonance (mr) imaging pattern suggestive of congenital cytomegalovirus (cmv) infection by using polymerase chain reaction (pcr) testing to detect cmv dna in neonatal blood on guthrie cards for validation. | 2004 | 14752192 |
| control of chemicals in sweden: an example of misuse of the "precautionary principle". | with a background in biochemistry and radiation biology, i started to get involved in the control of chemicals area by battling the use of alkyl-mercury compounds in swedish agriculture during the years 1964-1965 (c.-g. rosén, h. ackefors, and r. nilsson, 1966, seed dressing compounds based on organic mercury-economic aspects and health hazards, svensk kemisk tidskrift 78, 8-19), and subsequently i acted as the sole technical advisor to the plaintiffs for the thalidomide children in sweden for 4 ... | 2004 | 14759655 |
| cyclosporine treatment preserves coronary resistance artery function in rat cardiac allografts. | a marked decline in vascular myogenic response occurs during the course of rat cardiac allograft rejection. two important contributory features are an inducible nitrous oxide synthase (inos)-catalyzed, no-mediated vasodilation and a loss of smooth muscle function. in this study, we examine the effect of cyclosporine immunosuppressive therapy on the alleviation of arterial dysfunction of coronary resistance arteries in allografts using pressure myography. | 2004 | 14761767 |
| a comparative morphological and biological study of schistosoma haematobium, s. bovis, s.intercalatum fisher, 1934, s. mansoni and s. rodhaini brumpt, 1931. | | 1951 | 14878389 |
| isolation of nuclei from label-retaining cells and measurement of their turnover rates in rat colon. | we describe here a new technique for isolating nuclei from long-term label-retaining cells (lrcs), a subpopulation enriched with stem cells from colon, and for measuring their proliferation rates in vivo. a double-label approach was developed, combining the use of bromodeoxyuridine (brdu) and (2)h(2)o. male fisher 344 rats were administered brdu in drinking water continuously for 2-8 wk. brdu was then discontinued (brdu washout), and animals (n = 33) were switched to (2)h(2)o in drinking water a ... | 2004 | 14960413 |
| synthetic disialylgalactose immunoadsorbents deplete anti-gq1b antibodies from autoimmune neuropathy sera. | acute and chronic autoimmune neuropathies, including guillain-barré syndromes (gbs) are often characterized by the presence of autoantibodies that react with neural gangliosides. evidence from human and animal studies indicates that anti-ganglioside antibodies play a primary neuropathogenic role, and their rapid elimination from the circulation through specific immunoadsorption therapy thus has the potential to ameliorate the course of the disease. here we have tested this therapeutic principle ... | 2004 | 14960498 |
| the translocation of marrow mncs after experimental myocardial cryoinjury is proportional to the infarcted area. | the selective homing of peripherally injected marrow mncs (mmncs) has recently been demonstrated in a model of cryodamaged heart. however, the mechanism underlying this phenomenon is still unknown. in the hypothesis that this process is related to the necrotic area extension, the infarcted area was correlated with the number of homed mmncs in a model of experimental cryodamaged heart. | 2004 | 14962315 |
| focal astrocyte loss is followed by microvascular damage, with subsequent repair of the blood-brain barrier in the apparent absence of direct astrocytic contact. | blood-brain barrier (bbb) breakdown is a feature of cerebral ischaemia, multiple sclerosis, and other neurodegenerative diseases, yet the relationship between astrocytes and the bbb integrity remains unclear. we present a simple in vivo model in which primary astrocyte loss is followed by microvascular damage, using the metabolic toxin 3-chloropropanediol (s-alpha-chlorohydrin). this model is uncomplicated by trauma, ischaemia, or primary immune involvement, permitting the study of the role of a ... | 2004 | 14966864 |
| [prenatal and postnatal effects of dengue infection during pregnancy]. | the risk of dengue virus infection during pregnancy has increased due to the current rash of frequent and severe dengue epidemics. the effects of dengue virus in the fetus and newborn children have been studied only superficially and with contradictory results. therefore, a retrospective cohort study was conducted in medellin, colombia, to describe the fetal and postnatal effects of dengue virus infection acquired during pregnancy. twenty-two babies born from mothers who suffered dengue during t ... | 2003 | 14968920 |
| fructooligosaccharide associated with celecoxib reduces the number of aberrant crypt foci in the colon of rats. | according to burkitt's hypothesis, dietary fibres may protect against the development of colorectal cancer. in rats, studies have shown that only butyrate-producing fibres are protective. in parallel, in humans, non-steroidal anti-inflammatory drugs, which target cyclooxygenases, have been shown to display a protective effect against colorectal cancer. among them, cox-2-selective inhibitors which present less side effects than non-selective agents, are promising as chemopreventive agents. our ai ... | 2003 | 14971826 |
| substantial genetic effects involved in determination of circulating levels of calciotropic hormones in human pedigrees. | this paper reports the results of a series of univariate and bivariate statistical genetic analyses that were performed on a sample of nuclear and more complex pedigrees (n = 567 individuals) of an ethnically homogenous white population. our major objectives were: (1) to quantitatively evaluate the extent and pattern of the putative genetic effects on plasma level variation and covariation of the intact parathyroid hormone (pth) and 25-hydroxyvitamin d [25(oh)d]; (2) to evaluate the extent of th ... | 2003 | 14974678 |
| thyroid cancer immuno-therapy with retroviral and adenoviral vectors expressing granulocyte macrophage colony stimulating factor and interleukin-12 in a rat model. | introduction of genes encoding immuno-stimulatory cytokine(s) into cancer cells is well known to enhance anti-tumour immunity. | 2003 | 14974915 |
| a randomized trial of saline solution-moistened misoprostol versus dry misoprostol for first-trimester pregnancy failure. | the purpose of this study was to estimate whether the efficacy of treatment with intravaginal misoprostol for first-trimester pregnancy failure is enhanced by the addition of saline solution. | 2004 | 14981379 |
| analysis of mmp-1 and mmp-9 promoter polymorphisms in early osseointegrated implant failure. | polymorphisms, such as a guanine inserted at position -1607 in the promoter region of human matrix metalloprotenase 1 (mmp-1) or a c-1562t substitution in the mmp-9 gene, have been shown to increase the transcriptional activity of these mmps. the objective of this study was to investigate the possible relationship between these polymorphisms and early implant failure. | 2004 | 14982353 |
| involution of pten-null endometrial glands with progestin therapy. | loss of pten tumor suppressor gene function characterizes most (63%) endometrial precancerous lesions (endometrial intraepithelial neoplasia, ein) and up to 83% of endometrioid endometrial cancers. because systemic progestins are known to promote involution of precancerous endometrial lesions, we tested the hypothesis that this therapy preferentially leads to clearance of immunohistochemically detected pten-null endometrial glands in a variety of histopathologic settings. | 2004 | 14984979 |
| stereoselective metabolism and tissue retention in rats of the individual enantiomers of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal), metabolites of the tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk). | 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) is reduced to its main metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal) in a reaction that is both stereoselective and reversible. (s)-nnal has been shown to be equivalent to nnk in carcinogenic potency, and significantly more potent than (r)-nnal. it was hypothesized that stereoselective differences in metabolism or tissue distribution contributed to the difference in carcinogenicity between the enantiomers. the individual nna ... | 2004 | 14988218 |
| oxidation of vitamin e and vitamin c and inhibition of brain mitochondrial oxidative phosphorylation by peroxynitrite. | the effects of peroxynitrite (pn; product of the reaction between nitric oxide and superoxide) on mitochondrial respiration as well as oxidation of alpha-tocopherol and ascorbic acid were studied. mitochondria were isolated from brain hemispheres of 4-month-old male fisher rats by standard centrifugation procedures utilizing ficoll gradients. treatment of brain mitochondria with pn caused a concentration-dependent impairment of oxidative phosphorylation and depletion of the endogenous antioxidan ... | 2004 | 14994345 |
| experimental and finite element analysis of the rat ulnar loading model-correlations between strain and bone formation following fatigue loading. | the rat forelimb compression model has been used widely to study bone response to mechanical loading. we used strain gages to assess load sharing between the ulna and radius in the forelimb of adult fisher rats. we used histology and peripheral quantitative computed tomography (pqct) to quantify ulnar bone formation 12 days after in vivo fatigue loading. lastly, we developed a finite element model of the ulna to predict the pattern of surface strains during compression. our findings indicate tha ... | 2004 | 14996566 |
| electrical current-induced atrial and pulmonary vein action potential duration shortening and repetitive activity. | the influence of threshold electrical currents (ec) during regular drive on pulmonary vein (pv) and atrial myocardial cell action potential (ap) duration (apd) is unknown. we determined the effects of ec on cellular apd of pv, atria, and ventricles in isolated perfused and superfused male rat hearts (fisher-344 strain, 3-4 mo old) at 37 degrees c (n = 14). we determined apd changes caused by subthreshold and threshold ec synchronized with a distant pacing electrode and delivered nearby cells fro ... | 2004 | 15001438 |
| 3' utr (agg)n repeat of glial cell line-derived neurotrophic factor (gdnf) gene polymorphism in schizophrenia. | association studies on gene polymorphisms of neurotransmitter systems have hypothesized an involvement of dopamine receptors in susceptibility to schizophrenia. however, structural and morphological abnormalities in different brain regions of schizophrenic patients support neurodevelopmental etiology for schizophrenia and neurotrophic factor genes could be candidates for genetic studies. the glial cell line-derived neurotrophic factor (gdnf) is a neurotrophic and potential differentiation factor ... | 2004 | 15003293 |
| retrospective selection of elite parent trees using paternity testing with microsatellite markers: an alternative short term breeding tactic for eucalyptus. | the conventional way to drive modifications in old forest tree seed orchards is to establish progeny trials involving each parent tree and then evaluate its contribution to the performance of the progeny by estimating its general and specific combining ability (gca and sca). in this work, we successfully applied an alternative parent selection tactic based on paternity testing of superior offspring derived from a hybrid seed orchard established with a single eucalyptus grandis seed parents and s ... | 2004 | 15004676 |
| systemic humoral anti-helicobacter pylori immune response in patients with gastric malignancies and benign gastroduodenal disease. | helicobacter pylori elicits a strong local and systemic humoral immune response, but it is not able to eliminate bacteria. immune response may be important for the course of infection that may lead to different gastroduodenal disease. in order to investigate differences in systemic humoral immune response between patients with different gastroduodenal diseases, we conducted clinical and serological studies. | 2004 | 15011886 |
| [miller-fisher syndrome: clinical features, associated infections and clinical course in 8 cases]. | miller-fisher syndrome (mfs) is considered the most common variant of guillain-barré syndrome (gbs) and is characterized by the clinical triad of ophthalmoplegia, ataxia and areflexia. respiratory involvement and relapses are unusual. patients with mfs usually have a good recovery and no residual deficits. we describe the clinical features, associated infections and evolution in eight patients with mfs. | 2004 | 15012892 |
| glucagonlike peptide-2 analogue: a possible new approach in the management of inflammatory bowel disease. | glucagonlike peptide-2alpha (glp-2alpha) has been shown to be a growth factor for the small intestine. this study investigated the benefits of intravenous and intraluminal administration of glp-2alpha using a rat model of inflammatory bowel disease (ibd). | 2004 | 15017568 |
| did natural selection or genetic drift produce the cranial diversification of neotropical monkeys? | a central controversy among biologists is the relative importance of natural selection and genetic drift as creative forces shaping biological diversification (fisher 1930; wright 1931). historically, this controversy has been an effective engine powering several evolutionary research programs during the last century (provine 1989). while all biologists agree that both processes operate in nature to produce evolutionary change, there is a diversity of opinion about which process dominates at any ... | 2004 | 15026977 |
| effect of reactive oxygen species on the metabolism of tryptophan in rat brain: influence of age. | in an earlier study, oxidation of tryptophan hydroxylase was implicated as its affinity was decreased with aging in rat brain. to establish any potential link between its oxidative damage and aging, we have determined the activities of antioxidant enzymes in midbrain, pons and medulla of 2, 12 and 24 month old fisher 344 bnf1 rats. the results obtained suggest that the activities of antioxidant enzymes varied considerably with age and brain regions studied. activities of cu/zn superoxide dismuta ... | 2004 | 15030179 |
| rapid determination of vapa/vapb genotype in rhodococcus equi using a differential polymerase chain reaction method. | rhodococcus equi is a facultative pathogen of foals. infection causes an often fatal pulmonary pneumonia. the organism has also been isolated from pigs, cattle, humans and the environment. equine virulence has a high positive correlation with the expression of a 17.4 kd polypeptide of unknown function, vapa, the product of the plasmid-encoded vapa gene. more recently an isogene of vapa, referred to as vapb and encoding an 18.2 kda polypeptide, has been identified among pig and human isolates. th ... | 2004 | 15031644 |
| age-related changes of muscarinic cholinergic receptor subtypes in the striatum of fisher 344 rats. | striatum expresses a cholinergic system involved in the regulation of its activity and changes in striatal cholinergic receptors may be related to cognitive impairment. this study has investigated muscarinic cholinergic m1-m5 receptor subtype expression in striatum of fischer 344 rats aged 6 (young), 15 (adult) and 22 months (senescent) to assess the contribution of different muscarinic cholinergic receptor subtypes in age-related changes of striatal cholinergic neurotransmission. western blot a ... | 2004 | 15036415 |
| hiv-encephalopathy as initial manifestation of acquired immunodeficiency syndrome in yucatán state, mexico. | the frequency of neurologic manifestations of the central nervous system (cns) as a defining disease of acquired immunodeficiency syndrome (aids) (neurologic manifestation defining disease [nmdd-aids]) varies according to geographic region. the aim of this study was to determine its prevalence among patients with aids diagnosed in the state of yucatán, mexico. | 2004 | 15036800 |
| pharmacokinetics and tissue distribution of galantamine and galantamine-related radioactivity after single intravenous and oral administration in the rat. | the plasma kinetics and tissue distribution of galantamine hydrobromide [4as-(4a alpha,6beta,8ar*)]-4a,5,9,10,11,12-hexahydro-3-methoxy-11-methyl-6h-benzofuro-[3a,3,2-ef] [2benzazepin-6-ol hydrobromide, cas-1953-04-4], a reversible acetylcholinesterase inhibitor, were studied in male and female non-pregnant and pregnant spf wistar rats and in male fisher x copenhagen pigmented rats. most studies were performed using 3h-labelled galantamine hydrobromide, measuring unchanged drug (ud) and non-vola ... | 2004 | 15038457 |
| the effect of octreotide and bromocriptine on expression of a pro-apoptotic bax protein in rat prolactinoma. | it is well established that disruption of apoptosis may lead to tumor initiation, progression or metastasis. it is also well documented that many anticancer drugs induce apoptosis. in the earlier studies, the dopamine d2 receptor agonist bromocriptine (bc) and somatostatin analog octreotide (oct) were found to inhibit the growth of the estrogen-induced rat prolactinoma. our previous investigations, applying the tunel method showed the involvement of the pro-apoptotic effect in the action of bc, ... | 2004 | 15046398 |
| expression of eukaryotic initiation factor 4e in gastric adenocarcinoma and its association with clinical outcome. | overexpression of eif4e can result in oncogenic transformation and uncontrolled growth of mammalian cells, presumably by facilitating the expression of growth-control gene products, which are normally translationally repressed. overexpression of eif4e was present in human breast carcinoma and human head and neck squamous cell carcinoma, and may be of prognostic value in breast carcinomas. in order to elucidate the clinical significance of eif4e expression, this study was conducted to quantify ex ... | 2004 | 15048676 |
| dietary sodium gluconate protects rats from large bowel cancer by stimulating butyrate production. | butyrate has an antitumorigenic effect on colorectal cancer cell lines. dietary sodium gluconate (gna) promotes butyrate production in the large intestine. accordingly, we examined the effect of dietary gna on tumorigenesis in the large intestine in rats. male fisher-344 rats (n = 32) were divided into 4 groups: 2 diets (with or without 50 g gna/kg basal diet) x 2 treatments (with or without carcinogen administration). colonic tumors were induced by 3 intraperitoneal injections of azoxymethane ( ... | 2004 | 15051851 |
| [biotic and abiotic determinants of seroprevalence of antibodies against trypanosoma cruzi in palmar de bravo, puebla, mexico]. | to establish the relationship between seroprevalence for antibodies against trypanosoma cruzi and its relationship with biotic and abiotic factors. | 2004 | 15053395 |
| four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis. | identification of preneoplastic changes in histologically normal epithelium (the "field effect") could provide a powerful screening tool for colorectal cancer. however, to date, reliable detection has not been possible. we have recently developed a new generation of optical technology, 4-dimensional elastic light-scattering fingerprinting (4d-elf), which enables us to probe the nanoscale/microscale architecture of living cells. we therefore investigated whether 4d-elf would be able to identify p ... | 2004 | 15057746 |
| cure of fisher rats bearing radioresistant f98 glioma treated with cis-platinum and irradiated with monochromatic synchrotron x-rays. | high-grade gliomas are usually of poor prognosis, and conventional radiotherapy, even combined with chemotherapy, still fails to improve the survival of patients. here, we propose an innovative therapeutic approach combining synchrotron radiation with cis-diamminedichloroplatinum (ii) (cddp). as suggested previously, monochromatic synchrotron irradiation of cddp at 78.8 kev, just above the 78.4 kev platinum absorption k-edge, leads to an enhanced photoelectric effect and an increased local toxic ... | 2004 | 15059878 |
| [analysis of correlation between discrete genotype characteristics and quantitative morphological traits in oncorhynchus nerka (walbaum) from the kamchatka river]. | correlation of m, a, d quantitative parameters with a genotypic composition of ldh-b2* and pgm-2* loci in spawned population of the sockeye salmon from the kamchatka river has been analysed with the fisher-mather method (bezrukov, 1994). different values of m, a, d parameters were revealed for morphological characters depending on: 1) genotypic composition on each locus, 2) sexual composition of the population. the simple influence of heterozygosity was found on one locus for females and males s ... | 2003 | 15067946 |
| glucose-receptor mr imaging of tumors: study in mice with pegylated paramagnetic niosomes. | to evaluate a magnetic resonance (mr) imaging contrast agent for tumor detection based on paramagnetic nonionic vesicles (niosomes) bearing polyethylene glycol (peg) and glucose conjugates for the targeting of overexpressed glucose receptors. | 2004 | 15068944 |
| thymic myoid cells as a myasthenogenic antigen and antigen-presenting cells. | we investigated immune property of a myoid cell line, established from fisher rat thymus. immunization of syngeneic rats with the myoid cells induced anti-rat acetylcholine receptor (achr). implantation of them into the thymus failed to induce typical thymic pathology of human myasthenia gravis (mg) or anti-achr responses. we also demonstrated that the myoid cells were able to present exogenous antigens to t cells and induce antigen-specific t cell proliferation. these results suggest that myoid ... | 2004 | 15081251 |
| linkage disequilibrium in the domesticated pig. | this study investigated the extent of linkage disequilibrium (ld) in two genomic regions (on chromosomes 4 and 7) in five populations of domesticated pigs. ld was measured with d' and tested for significance with the fisher exact test. effects of genetic (linkage) distance, chromosome, population, and their interactions on d' were tested both through a linear model analysis of covariance and by a theoretical nonlinear model. the overall result was that (1) the distance explained most of the vari ... | 2004 | 15082558 |
| mr imaging findings of spinal posterior column involvement in a case of miller fisher syndrome. | summary: the site of lesions causing ataxia in miller fisher syndrome (mfs) remains in dispute. a 43-year-old man manifested rapidly progressive left-sided ptosis, bilateral abducens palsy, areflexia, and severe ataxia. initial mr imaging showed confined lesions of the cauda equina with gadolinium enhancement. a diagnosis of mfs was made, and the patient underwent immunotherapy. his ophthalmoplegia disappeared, but other symptoms remained. five months after onset, mr imaging disclosed lesions co ... | 2004 | 15090361 |
| stone age hut in israel yields world's oldest evidence of bedding. | the earliest archaeological remains of dwelling huts built by homo sapiens were found in various european upper paleolithic open-air camps. although floors of huts were found in a small number of cases, modern organization of the home space that includes defined resting areas and bedding remains was not discovered. we report here the earliest in situ bedding exposed on a brush hut floor. it has recently been found at the previously submerged, excellently preserved 23,000-year-old fisher-hunter-g ... | 2004 | 15090648 |
| hepatic hmg-coa reductase expression and resistance to dietary cholesterol. | the premise that the intrinsic level of expression of hepatic 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase determines the relative sensitivity to the serum cholesterol raising action of dietary cholesterol was examined in 9 strains of rat. for further comparison purposes, hamsters were also examined. the basal expression of hepatic hmg-coa reductase, extent of feedback regulation by cholesterol, and changes in serum cholesterol levels and the hepatic low-density lipoprotein (ldl) re ... | 2004 | 15096653 |
| determinants of enrollment in a preventive hiv vaccine trial: hypothetical versus actual willingness and barriers to participation. | to compare hypothetical and actual willingness to enroll in a preventive hiv vaccine trial and identify factors affecting enrollment. | 2004 | 15097304 |
| impact of aging on gene expression in a rat model of ischemic cutaneous wound healing. | tissue ischemia and aging are independent features associated with the healing impairment of cutaneous wounds. however, the pathophysiology of these processes as they relate to impaired-healing wounds is poorly understood. | 2004 | 15100008 |
| evidence for an association between chlamydia psittaci and ocular adnexal lymphomas. | ocular adnexal lymphomas may be antigen-driven disorders; however, the source of the putative antigen or antigens is still unknown. hence, we assessed whether chlamydiae infection is associated with the development of ocular adnexal lymphomas. | 2004 | 15100336 |
| multidrug-resistant tuberculosis versus drug-sensitive tuberculosis in human immunodeficiency virus-negative patients: computed tomography features. | to compare the computed tomography (ct) features of patients with multidrug-resistant tuberculosis with those of patients with drug-sensitive tuberculosis in a country not associated with the human immunodeficiency virus (hiv) epidemic. | 2004 | 15100542 |
| attenuation of cerebral vasospasm by systemic administration of an endothelin-a receptor antagonist, tbc 11251, in a rabbit model of subarachnoid hemorrhage. | cerebral vasospasm is a major complication of subarachnoid hemorrhage (sah) after the rupture of an intracranial aneurysm. although the cause of cerebral vasospasm has not been fully established, several lines of evidence suggest that the vasoconstrictor peptide endothelin (et) may play a crucial role. in the present study the potential of tbc 11251 (tbc), a newly developed et(a) receptor antagonist, to prevent and/or reverse cerebral vasospasm was examined in a well-established rabbit model of ... | 1997 | 15104411 |
| [nail disease due to scytalidium in martinique (french west indies)]. | scytalidium is an endemic mold in tropical and subtropial areas. our purpose was to study the prevalence and clinical and epidemiological features of onychomycoses due to scytalidium in martinique (french west indies). | 2004 | 15107741 |
| anti-tubular basement membrane antibodies and giant cell formation in a model of chronic renal allograft rejection. | in the fisher (f344)-to-lewis (lew) kidney transplantation (tx) model of chronic rejection, antibodies reactive with tubular basement membranes have been found. we investigated whether giant cells, typical for a granulomatous inflammatory reaction, can be found during allograft rejection. | 2004 | 15114101 |
| [association of estrogen and progesterone receptor levels and the expected response to chemotherapy and hormone therapy in breast carcinoma with increased expression of human epidermal growth factor 2]. | the predictive value of human epidermal growth factor receptor 2 (her-2) on the response to chemotherapy and endocrine therapy in breast cancer patients has not yet been determined. the expression of other biomarkers in breast cancer can further influence the response to therapy. the aim of our study was to investigate if status of steroid receptors (sr) influenced the response to anthracycline-containing chemotherapy and tamoxifen in a group of her-2 positive advanced breast cancer patients. fo ... | 2003 | 15114785 |
| management of infant diarrhea with high-lactose probiotic-containing formula. | to study the influence of high-lactose probiotic-containing formula on the course of acute diarrhea, an experiment using a randomized controlled clinical trial with patients having acute diarrhea for 3 days was conducted. one hundred patients were allocated into two groups that were comparable for age, sex, and nutritional status. the test group was administered high-lactose bifidobacterium bifidum-containing formula, while the control group had no high-lactose probiotic until the end of the exp ... | 2003 | 15115098 |
| molecular targeting of lymph nodes with l-selectin ligand-specific us contrast agent: a feasibility study in mice and dogs. | to evaluate the feasibility of using intravenously administered l-selectin ligand-specific polymer-stabilized air-filled microparticles (mps) for active targeting of peripheral lymph nodes under normal conditions in animal models. | 2004 | 15118116 |
| experimental intrauterine infection with prevotella bivia in new zealand white rabbits. | the purpose of this study was to develop a model of chronic intrauterine and fetal infection with prevotella bivia, an anaerobe of the lower genital tract that is associated often with bacterial vaginosis. | 2004 | 15118646 |
| function and clustered expression of maxik channels in cerebral myocytes remain intact with aging. | the incidence of stroke increases significantly in the aging population where stroke related deaths boost at >75 years and survivors are often permanently disabled. aging is known to decrease cerebral blood flow likely due to an increase in arterial tone. although maxik channels are key regulators of cerebral arterial tone their pattern of expression and function in cerebral blood vessels during aging is unknown. using specific antibodies against the alpha-subunit of maxik channels and current r ... | 2004 | 15130678 |
| delayed decompressive surgery increases apparent diffusion coefficient and improves peri-infarct perfusion in rats with space-occupying cerebral infarction. | there is no conclusive experimental support that decompressive surgery in late stages of space-occupying cerebral infarction will improve outcome. we studied the effects of delayed decompressive surgery on the development of tissue damage, edema formation, and cerebral perfusion with different mri techniques in a rat model of space-occupying cerebral infarction. | 2004 | 15131314 |
| isoliquiritigenin, a flavonoid from licorice, reduces prostaglandin e2 and nitric oxide, causes apoptosis, and suppresses aberrant crypt foci development. | isoliquiritigenin (iltg), a flavonoid group compound, exists in some foodstuffs and herbal medicines such as licorice (glycyrrhiza uralensis fisher). previously, we showed that iltg can suppress azoxymethane (aom)-induced colon carcinogenesis in ddy mice. in the present report, we present evidence that iltg markedly decreases both prostaglandin e2 (pge2) and nitric oxide (no) production in raw264.7 mouse macrophage cells. the decrease of pge2 was dependent on cyclooxygenase-2 (cox-2) expression ... | 2004 | 15132774 |
| lactic acidosis after cardiac surgery is associated with adverse outcome. | the accurate identification of patients who have the potential to further deteriorate after cardiac surgery is difficult. elevated serum lactate level after cardiac surgery is an indicator of systemic hypoperfusion and tissue hypoxia. the aim of this study was to investigate the effect of increased serum lactate on outcome after on-pump coronary artery bypass grafting. | 2004 | 15138095 |
| comparison of eps' and psychiatrists' laboratory assessment of psychiatric patients. | eps frequently transfer psychiatric patients to psychiatric hospitals with the approval and acceptance of a psychiatrist. eps and psychiatrists have an unknown set of testing routines and requirements that are used to determine medical clearance of the psychiatric patient. the purpose of this study was to compare the routine and required testing performed by eps as compared with psychiatrists. a survey of routine and required test ordering for the medical clearance of patients presenting with ps ... | 2004 | 15138952 |
| efficacy of sodium hypochlorite and coconut soap used as disinfecting agents in the reduction of denture stomatitis, streptococcus mutans and candida albicans. | this study evaluated the reduction of denture stomatitis and the antimicrobial activity of 0.05% sodium hypochlorite opposed to candida albicans and streptococcus mutans (sgm) when associated with brushing complete dentures with coconut soap. the mucosal characteristics were evaluated according to newton's classification at baseline, after cleansing the dentures with coconut soap for 15 days in group 1 (nine patients). in the other group (19 patients) the analysis were made before and after clea ... | 2004 | 15140171 |
| human anti-anthrax protective antigen neutralizing monoclonal antibodies derived from donors vaccinated with anthrax vaccine adsorbed. | background: potent anthrax toxin neutralizing human monoclonal antibodies were generated from peripheral blood lymphocytes obtained from anthrax vaccine adsorbed (ava) immune donors. the anti-anthrax toxin human monoclonal antibodies were evaluated for neutralization of anthrax lethal toxin in vivo in the fisher 344 rat bolus toxin challenge model. methods: human peripheral blood lymphocytes from ava immunized donors were engrafted into severe combined immunodeficient (scid) mice. vaccination wi ... | 2004 | 15140257 |
| evaluation of bronchoalveolar lavage fluid cytotoxic parameters after inhalation exposure to amosite and wollastonite fibrous dusts combined with cigarette smoke. | the aim of this work was to compare the influence of amosite-asbestos and wollastonite fibrous dusts combined with cigarette smoke on chosen cytotoxic parameters of bronchoalveolar lavage fluid (balf) in rats. fisher 344 rats inhaled wollastonite or amosite fibrous dusts (60 or 30 mg x m(-3) air) one hour every two days combined with daily breathing of diluted mainstream tobacco smoke (30 mg of tpm x m(-3) air). the experiment lasted 6 months. after sacrifying the animals bronchoalveolar lavage ... | 2004 | 15141967 |
| the effect of helicobacter pylori infection on apoptosis and cell proliferation in gastric epithelium. | helicobacter pylori (hp) may affect the normal balance between gastric epithelial cell proliferation and epithelial cell death, interfering thus with the maintenance of gastric mucosa integrity. the aim of this study was to investigate the effect of hp infection on cell proliferation index (pi) and apoptotic index (ai) in gastric epithelium of the antrum and corpus. | 2004 | 15143938 |
| prior serum- and aicar-induced ampk activation in primary human myocytes does not lead to subsequent increase in insulin-stimulated glucose uptake. | exposing isolated rat skeletal muscle to 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside [aicar, a pharmacological activator of amp-activated protein kinase (ampk)] plus serum leads to a subsequent increase in insulin-stimulated glucose transport (fisher js, gao j, han dh, holloszy jo, and nolte la. am j physiol endocrinol metab 282: e18-e23, 2002). our goal was to determine whether preincubation of primary human skeletal muscle cells with human serum and aicar (serum+aicar) would also in ... | 2004 | 15149951 |
| sleep, but not febrile responses of fisher 344 rats to immune challenge are affected by aging. | sleep is altered in response to infection and immune challenge in humans and non-human animals. although there are changes in sleep and facets of immune function with aging, sleep responses of aged subjects to immune challenge have received little, if any attention. to test the hypothesis that aging affects sleep responses to immune challenge, intracerebroventricular injections of interleukin 1 (il-1) were given to young and aged rats and subsequent sleep-wake behavior was determined. under basa ... | 2004 | 15157957 |
| development and characterization of enhanced green fluorescent protein and luciferase expressing cell line for non-destructive evaluation of tissue engineering constructs. | this study investigates the utility of genetically modified cells developed for the qualitative and quantitative non-destructive evaluation of cells on biomaterials. the fisher rat fibroblastic cell line has been genetically modified to stably express the reporter genes enhanced green fluorescence protein (egfp) and luciferase. these reporter genes provide two unique opportunities to evaluate cell growth on materials without destruction of the sample. utilizing the fluorescence of egfp expressed ... | 2004 | 15172493 |
| measuring the relative information in allele-sharing linkage studies. | in the context of allele-sharing methods, this article investigates ways of measuring the information in the marker data relative to the amount of information that would have been available if the identity-by-descent (ibd) process were known. such measures are needed to decide whether new markers can substantially modify the evidence for excess sharing. we propose new measures that take advantage of the properties of the exponential model introduced by kong and cox (1997, american journal of hum ... | 2004 | 15180662 |
| a p2x7 receptor stimulates plasma membrane trafficking in the frtl rat thyrocyte cell line. | thyroid cells express a variety of p2y and p2x purinergic receptor subtypes. g protein-coupled p2y receptors influence a wide variety of thyrocyte-specific functions; however, functional p2x receptor-gated channels have not been observed. in this study, we used whole cell patch-clamp recording and fluorescence imaging of the plasma membrane marker fm1-43 to examine the effects of extracellular atp on membrane permeability and trafficking in the fisher rat thyroid cell line frtl. we found a catio ... | 2004 | 15189815 |
| the time course of ischemic damage and cerebral perfusion in a rat model of space-occupying cerebral infarction. | we aimed to establish a rat model of space-occupying hemispheric infarction to evaluate potential treatment strategies. for adequate timing of therapy in future experiments, we studied the development of tissue damage, edema formation, and perfusion over time with different mri techniques. permanent middle cerebral artery (mca) occlusion was performed in 32 fisher-344 rats. forty-six mri experiments including diffusion weighted (dw), t2-weighted (t2w), flow-sensitive alternating inversion recove ... | 2004 | 15196969 |
| unexpected severe calcification after transplantation of bone marrow cells in acute myocardial infarction. | there has been a rapid increase in the number of clinical trials using unselected bone marrow (bm) cells or the mononuclear fraction of bm cells for treating ischemic heart diseases. thus far, no significant deleterious effects or complications have been reported in any studies using bm-derived cells for treatment of various cardiac diseases. | 2004 | 15197139 |
| 15-deoxy-delta12,14-prostaglandin j2-mediated erk signaling inhibits gram-negative bacteria-induced rela phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2a activity. | we have previously shown that non-pathogenic gram-negative bacteroides vulgatus induces transient rela phosphorylation (ser-536), nf-kappab activity, and pro-inflammatory gene expression in native and intestinal epithelial cell (iec) lines. we now demonstrate that 15-deoxy-delta(12,14)-prostaglandin j(2) (15d-pgj(2)) but not prostaglandin e(2) inhibits lipopolysaccharide (lps) (b. vulgatus)/lps (escherichia coli)-induced rela phosphorylation and interleukin-6 gene expression in the colonic epith ... | 2004 | 15199053 |
| vitamin a prevents high fat diet-induced acf development and modifies the pattern of expression of peroxisome proliferator and retinoic acid receptor m-rna. | some dietary compounds, among them fats, are modulators of colon cancer risk. this study reports the modulating effects of n-6, with or without vitamin a, on promotion of colon preneoplasic lesions induced by 1,2-dimethylhydrazine (dmh) and on the expression of nuclear receptors (ppargamma, rxralpha, and rarbeta). one group of male fisher rats was fed a basic diet (5% safflower oil) and two groups were fed a high-fat diet (hfd, 25% safflower oil). of these, one was supplemented with 200 iu vitam ... | 2004 | 15203375 |
| fty720 prolongs clear corneal allograft survival with a differential effect on different lymphocyte populations. | fty720 is a potent immunomodulator with unique effects on lymphocyte homing and has recently proved to be safe and effective in renal transplantation in man. the authors investigated the potency of fty720 in inhibiting allograft rejection in the rat model of orthotopic allogeneic penetrating keratoplasty. | 2004 | 15205237 |
| bioactivation, toxicokinetics and acute effects of chloroform in fisher 344 and osborne mendel male rats. | chloroform has been regarded as a renal carcinogen, based on results obtained with osborne mendel (om) rats. fisher 344 (f344) rats, considered representative of om rats on the basis of comparable acute toxic effects, have been used in most of the studies aimed to elucidate the mechanisms of kidney tumour induction. in the present work, in vitro and in vivo chloroform bioactivation in the liver and kidney of f344 and om rats has been reported, as well as additional toxicokinetics and acute toxic ... | 2004 | 15211614 |
| overexpression of human carcinoma-associated antigen in urothelial carcinoma of the bladder. | human carcinoma-associated antigen (hca) is a mucin protein whose level is increased in the sera of patients with a variety of carcinomas. we have previously shown that prostatic carcinoma overexpresses hca in comparison to benign prostatic tissue. to our knowledge, expression of hca in other tumors has not been reported previously. | 2004 | 15214822 |
| infected aortic aneurysms: clinical outcome and risk factor analysis. | infected aortic aneurysms are difficult to treat, and are associated with significant mortality. hospital survival is poor in patients with severe aortic infection, salmonella species infection, staphylococcus aureus infection, aneurysm rupture, and suprarenal aneurysm location. we reviewed the clinical outcome in 46 patients with primary infected aortic aneurysms and identified clinical variables associated with prognosis. | 2004 | 15218459 |
| transforming growth factor-alpha and nonsyndromic cleft lip with or without palate in brazilian patients: results of a large case-control study. | transforming growth factor-alpha (tgfa) was the first gene suggested to be associated with nonsyndromic cleft lip, cleft palate, or both (cl/ p). there are, however, still controversies of the effect of tgfa on the predisposition of this malformation. to contribute to a better understanding of the role of this gene in the occurrence of cl/p we undertook a case-control study including patients and controls ascertained in different regions of the country. | 2004 | 15222785 |
| regional induction of cyp1a1 in rat liver following treatment with mixtures of pcb 126 and pcb 153. | liver enzyme induction has been shown previously to be regional with clear borders between induced and uninduced regions in vivo, and cells either fully induced or not induced in vitro. the current study examined this phenomenon in vivo by evaluating enzyme induction after exposure to pcb 126 and pcb 153 in female fisher 344 (f344) and male sprague-dawley (sd) rats. ihc revealed a regional induction of cyp1a1 after exposure to pcb 126, apparent in the centrilobular region at lower doses and prog ... | 2004 | 15223772 |
| novel mechanism of inhibition of hiv-1 reverse transcriptase by a new non-nucleoside analog, km-1. | 2-naphthalenesulfonic acid (4-hydroxy-7-[[[[5-hydroxy-6-[(4 cinnamylphenyl)azo]-7-sulfo-2-naphthalenyl]amino]-carbonyl]amino]-3-[(4-cinnamylphenyl)]azo (km-1)) is a novel non-nucleoside reverse transcriptase inhibitor (nnrti) that was designed to bind at an unconventional site on human immunodeficiency virus type 1 reverse transcriptase (rt) (skillman, a. g., maurer, k. w., roe, d. c., stauber, m. j., eargle, d., ewing, t. j., muscate, a., davioud-charvet, e., medaglia, m. v., fisher, r. j., arn ... | 2004 | 15231830 |
| microarray analysis of thyroid hormone-induced changes in mrna expression in the adult rat brain. | to determine which genes in the adult rat brain are regulated by thyroid hormone (th), we used microarrays to examine the effect of hyperthyroidism on neuron-specific gene expression. four-month-old male fisher 344 rats were rendered hyperthyroid by intraperitoneal injection of 3,5,3'-l-triiodothyronine (t3, 15 microg/100 g body weight) for 10 consecutive days. to minimize interindividual variability, pooled cerebral tissue rna from four-control and five-hyperthyroid rats was hybridized in dupli ... | 2004 | 15234464 |
| prostate carcinoma among men with human immunodeficiency virus infection. | several malignancies are known to occur more frequently in individuals with human immunodeficiency virus (hiv) infection. to determine the incidence of prostate carcinoma in men with hiv infection, the authors initiated a prostate carcinoma screening program in a large hiv clinic. | 2004 | 15241826 |
| apoptotic pathway related to oval cell proliferation. | oval cells, liver stem cell-derived cells, are generated from the liver periportal region and spread into the parenchyma by an autocrine signaling pathway. the mechanism behind how oval cells take their place among packed silent hepatocytes, however, is not well understood. we hypothesized that apoptosis involves a decrease in hepatocytes surrounding oval cells. | 2004 | 15242488 |
| biopolymer-mediated suramin chemotherapy in the treatment of experimental brain tumours. | suramin inhibits tumour growth and neoangiogenesis by blocking several growth factor receptors. in this study the toxicity and efficacy of intralesional delivery of suramin incorporated in a controlled-release polymer were assessed in a rat 9l tumour model. initially, the toxicity of the compound was evaluated in adult fisher 344 rats. the animals were intracerebrally implanted with an ethylene vinyl acetate copolymer. these experiments showed early toxicity in the rats implanted with a 50% load ... | 2004 | 15244249 |
| glutathione metabolism during aging and in alzheimer disease. | the concentration of glutathione (gsh), the most abundant intracellular nonprotein thiol and important antioxidant, declines with age and in some age-related diseases. the underlying mechanism, however, is not clear. the previous studies from our laboratory showed that the age-dependent decline in gsh content in fisher 344 rats was associated with a downregulation of glutamate cysteine ligase (gcl), the rate-limiting enzyme in de novo gsh synthesis. our recent studies further indicated that the ... | 2004 | 15247041 |
| physical conditioning modulates rat cardiac vascular endothelial growth factor gene expression in nitric oxide-deficient hypertension. | many individuals with cardiac diseases undergo periodic physical conditioning with or without medication to improve cardiovascular health. therefore, this study investigated the interaction of physical training and chronic nitric oxide synthase (nos) inhibitor (nitro-l-arginine methyl ester, l-name) treatment on blood pressure (bp), cardiac vascular endothelial factor (vegf) gene expression, and nitric oxide (no) systems in rats. fisher 344 rats were divided into four groups and treated as follo ... | 2004 | 15249212 |
| aminoguanidine prevents age-related deterioration in left ventricular-arterial coupling in fisher 344 rats. | in recent studies, aminoguanidine (ag), an inhibitor of advanced glycation endproducts, has been identified as a prominent agent that can prevent the age-related aortic stiffening and cardiac hypertrophy. the aim of this study was to determine whether ag had effects on the left ventricular (lv)-arterial coupling in aged fisher 344 rats in terms of the ventricular and arterial chamber properties. normotensive rats were treated from 18 to 24 months with ag (1 g l(-1) in drinking water) and compare ... | 2004 | 15249427 |
| thyroid-stimulating antibody assay using a human thyrotropin receptor transfected cell line: relationship to clinical features of graves' disease. | to evaluate a chinese hamster ovary cell line transfected with the human thyrotropin (thyroid-stimulating hormone or tsh) receptor (cho-tshr) for its use in routine clinical testing and compare it with the currently used fisher rat thyroid cell line (frtl-5) relative to the cyclic adenosine monophosphate (camp) responses to thyroid-stimulating antibodies (tsab). | 1997 | 15251770 |
| miller fisher syndrome--a presenting clinical manifestation of lung cancer in a previously apparently healthy individual. | | 2004 | 15258802 |
| anti-gq1b ganglioside antibody in peripheral nervous system disorders: pathophysiologic role and clinical relevance. | the recent literature about autoimmune peripheral neuropathies has been dominated by the discovery of antibodies to a variety of glycosphingolipids. gangliosides are important carbohydrate determinants for autoimmune activity, and several studies have suggested that serum antibodies against gangliosides are responsible for some forms of acute and chronic neuropathy syndromes. however, this view is disputable, and despite substantial progress in understanding the potential pathogenic effects of a ... | 2004 | 15262730 |
| [genetic and microbiologic tests in periodontal disease]. | the presence of genetic polymorphisms il-1a and il-1b was demonstrated to be associated to an increased clinical severity of periodontitis. the aim of our study was to verify the existence of a relationship between such genetic polymorphisms and pathogenic variations of actinobacillus actinomycetemcomitans (aa), prevotella intermedia (pi) and porphyromonans gingivalis (pg), in patients with periodontitis before and after active therapy. | 2004 | 15266289 |
| a clinicopathologic evaluation of follicular lymphoma grade 3a versus grade 3b reveals no survival differences. | the world health organization classification recommends categorizing grade 3 follicular lymphomas based on the presence of centrocytes (grade 3a) or of sheets of centroblasts (grade 3b). the clinical significance of this practice is not known. | 2004 | 15270618 |
| conflict and impacts of divers and anglers in a marine park. | the new south wales state government (australia) gazetted the jervis bay marine park (jbmp) in 1998. during the preparation of the draft zoning plan in 2000, societal data on two conflicting park user groups--recreational scuba divers and fishers (anglers)--was collected. while conflict resolution was a plan priority, other factors, such as cumulative environmental impacts of users and protection for the critically endangered grey nurse shark (carcharias taurus), further complicated planning. bo ... | 2004 | 15285398 |
| total hcv core antigen assay. a new marker of hcv viremia and its application during treatment of chronic hepatitis c. | the present study assesses the clinical usefulness of the hepatitis c core antigen assay for monitoring of patients being treated for chronic hepatitis c virus (hcv) infection. eighty-six serum samples were selected at random from 16 patients and levels of hcv rna and hcv core antigen were determined simultaneously and in parallel to compare both techniques. the data obtained were compared by pearson's correlation and the coefficients calculated by fisher transformation and by calculating the di ... | 2004 | 15288960 |
| neural progenitor cell lines inhibit rat tumor growth in vivo. | current therapies for gliomas often fail to address their infiltrative nature. conventional treatments leave behind small clusters of neoplastic cells, resulting in eventual tumor recurrence. in the present study, we have evaluated the antitumor activity of neural progenitor cells against gliomas when stereotactically injected into nucleus caudatus of fisher rats. we show that the rat neural progenitor cell lines hib5 and st14a, from embryonic hippocampus and striatum primordium, respectively, a ... | 2004 | 15289341 |
| an in vitro study evaluating the effect of ferrule length on fracture resistance of endodontically treated teeth restored with fiber-reinforced and zirconia dowel systems. | there are few published studies analyzing the effects of different ferrule lengths of endodontically treated teeth in relationship to newly developed fiber-reinforced and zirconia dowel systems. | 2004 | 15295325 |
| x-ray structure of the signal transduction protein from escherichia coli at 1.9 a. | the structure of the bacterial signal transduction protein p(ii) has been refined to an r factor of 13.2% using 3sigma data between 10 and 1.9 a. the crystals exhibited twinning by merohedry and x-ray intensities were corrected using the method of fisher & sweet [fisher & sweet (1980). acta cryst. a36, 755-760] prior to refinement. our earlier 2.7 a structure [cheah, carr, suffolk, vasudevan, dixon & ollis (1994). structure, 2, 981-990] served as a starting model. p(ii) is a trimeric molecule, e ... | 1996 | 15299730 |
| analysis of cerebral microvascular architecture--application to cortical and subcortical vessels in rat brain. | cerebral vascular morphology, including vascular diameters, lengths and branch points, was measured using quantitative three-dimensional (3d) imaging software developed in our laboratory, where data were collected from cortical and subcortical regions of a rat brain. to compare multiple records of the vascular morphological differences between anatomical regions with unpaired data, a simple analysis of variance (anova) model may not be applicable, or may not be valid without considering correlat ... | 2004 | 15325115 |
| reversible disruption of tight junction complexes in the rat blood-brain barrier, following transitory focal astrocyte loss. | breakdown of the blood-brain barrier is a feature of acute and chronic neurodegenerative changes, yet the relationship between astrocytes and the mature barrier remains unclear. we studied this role of astrocytes in vivo using a gliotoxin and evaluated changes in three vascular tight junction markers. male fisher f344 rats given systemic 3-chloropropanediol showed astrocytic loss in the inferior colliculus from 12-24 h until the lesion was repopulated 8-28 days later. within 6 h of astrocyte los ... | 2004 | 15326610 |
| protective effects of fty720 on chronic allograft nephropathy by reducing late lymphocytic infiltration. | lymphocytic infiltration is obvious throughout early and late stages of chronic allograft nephropathy. early infiltrating lymphocytes are involved in initial insults to kidney allografts, but the contribution of late infiltration to long-term allograft attrition is still controversial. early application of fty720 reduced the number of graft infiltrating lymphocytes, and inhibited acute rejection. the present study investigated the potential of fty720 to reduce the number of infiltrating lymphocy ... | 2004 | 15327424 |
| platelet gp iiia polymorphism hpa-1 (pla) protects against subarachnoid hemorrhage. | few genetic modifications have been identified to be associated with subarachnoid hemorrhage (sah), most of them playing a role in the formation or size of aneurysms. | 2004 | 15331787 |