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earthworm-mediated synthesis of silver nanoparticles: a potent tool against hepatocellular carcinoma, plasmodium falciparum parasites and malaria mosquitoes.the development of parasites and pathogens resistant to synthetic drugs highlighted the needing of novel, eco-friendly and effective control approaches. recently, metal nanoparticles have been proposed as highly effective tools towards cancer cells and plasmodium parasites. in this study, we synthesized silver nanoparticles (ew-agnp) using eudrilus eugeniae earthworms as reducing and stabilizing agents. ew-agnp showed plasmon resonance reduction in uv-vis spectrophotometry, the functional groups ...201626873539
multipurpose effectiveness of couroupita guianensis-synthesized gold nanoparticles: high antiplasmodial potential, field efficacy against malaria vectors and synergy with aplocheilus lineatus predators.mosquito-borne diseases represent a deadly threat for millions of people worldwide. according to recent estimates, about 3.2 billion people, almost half of the world's population, are at risk of malaria. malaria control is particularly challenging due to a growing number of chloroquine-resistant plasmodium and pesticide-resistant anopheles vectors. newer and safer control tools are required. in this research, gold nanoparticles (aunps) were biosynthesized using a cheap flower extract of couroupi ...201626732702
fern-synthesized nanoparticles in the fight against malaria: lc/ms analysis of pteridium aquilinum leaf extract and biosynthesis of silver nanoparticles with high mosquitocidal and antiplasmodial activity.malaria remains a major public health problem due to the emergence and spread of plasmodium falciparum strains resistant to chloroquine. there is an urgent need to investigate new and effective sources of antimalarial drugs. this research proposed a novel method of fern-mediated synthesis of silver nanoparticles (agnp) using a cheap plant extract of pteridium aquilinum, acting as a reducing and capping agent. agnp were characterized by uv-vis spectrophotometry, fourier transform infrared (ftir) ...201626612497
seaweed-synthesized silver nanoparticles: an eco-friendly tool in the fight against plasmodium falciparum and its vector anopheles stephensi?malaria, the most widespread mosquito-borne disease, affects 350-500 million people each year. eco-friendly control tools against malaria vectors are urgently needed. this research proposed a novel method of plant-mediated synthesis of silver nanoparticles (agnp) using a cheap seaweed extract of ulva lactuca, acting as a reducing and capping agent. agnp were characterized by uv-vis spectrophotometry, fourier transform infrared (ftir) spectroscopy, energy-dispersive x-ray spectroscopy (edx), scan ...201526227141
mosquitocidal and antiplasmodial activity of senna occidentalis (cassiae) and ocimum basilicum (lamiaceae) from maruthamalai hills against anopheles stephensi and plasmodium falciparum.each year, mosquito-borne diseases infect nearly 700 million people, resulting to more than 1 million deaths. in this study, we evaluated the larvicidal, pupicidal, and smoke toxicity of senna occidentalis and ocimum basilicum leaf extracts against the malaria vector anopheles stephensi. furthermore, the antiplasmodial activity of plant extracts was evaluated against chloroquine (cq)-resistant (cq-r) and cq-sensitive (cq-s) strains of plasmodium falciparum. in larvicidal and pupicidal experiment ...201526122992
a sensitive enhanced chemiluminescent-elisa for the detection of plasmodium falciparum circumsporozoite antigen in midguts of anopheles stephensi mosquitoes.efforts to develop a successful malaria vaccine are hampered due to lack of assays that are predictive of protective immunity without conducting large clinical studies. the effect of experimental vaccines and drugs on malaria transmission is yet more difficult to measure. knowledge on the plasmodium infection rate in mosquito populations will aid the measurement of effects from intervention measures for malaria control. here, we report the development of a chemiluminescent sandwich elisa (ecl-el ...201525455023
population dynamics of mosquitoes and malaria vector incrimination in district charsadda, khyber pakhtunkhwa (kp) pakistan.this study aimed to obtain updated information on mosquito diversity and malaria vector incrimination in charsadda khyber pakhtunkhwa to help in devising effective control and preventive measures in the area. monthly survey of indoor mosquitoes for one year was carried out in three villages, dhaki totaqi and mathra. female anopheline were used to detect circumsporozoites protein (csp) using enzyme linked immunosorbent assay. among 17 mosquito species, culex quinquefasciatus, anopheles splendidus ...201525255965
first record of the asian malaria vector anopheles stephensi and its possible role in the resurgence of malaria in djibouti, horn of africa.anopheles stephensi is an important vector of urban malaria in india and the persian gulf area. its previously known geographical range includes southern asia and the arab peninsula. for the first time, we report a. stephensi from the african continent, based on collections made in djibouti, on the horn of africa, where this species' occurrence was linked to an unusual urban outbreak of plasmodium falciparum malaria, with 1228 cases reported from february to may 2013, and a second, more severe e ...201425004439
a scalable assessment of plasmodium falciparum transmission in the standard membrane-feeding assay, using transgenic parasites expressing green fluorescent protein-luciferase.the development of drugs and vaccines to reduce malaria transmission is an important part of eradication plans. the transmission-reducing activity (tra) of these agents is currently determined in the standard membrane-feeding assay (smfa), based on subjective microscopy-based readouts and with limitations in upscaling and throughput.201424829466
wolbachia invades anopheles stephensi populations and induces refractoriness to plasmodium infection.wolbachia is a maternally transmitted symbiotic bacterium of insects that has been proposed as a potential agent for the control of insect-transmitted diseases. one of the major limitations preventing the development of wolbachia for malaria control has been the inability to establish inherited infections of wolbachia in anopheline mosquitoes. here, we report the establishment of a stable wolbachia infection in an important malaria vector, anopheles stephensi. in a. stephensi, wolbachia strain w ...201323661760
comparative susceptibilities of species t and u of the anopheles fluviatilis complex to plasmodium vinckei petteri sporogony.anopheles fluviatilis james is an important malaria vector in indian subcontinent. an. fluviatilis exists as a complex of three sibling species, of which two species, t and u, have been colonized so far. attempts were made to study the comparative susceptibility of species t and u of the an. fluviatilis complex to rodent malaria parasite plasmodium vinckei petteri by using anopheles stephensi liston as calibrator for variable infectivity in different isolates. an. stephensi, which was used as co ...201323802454
the footprints of relapsing malaria in southwest delhi, india.control of vivax malaria is challenging due to persistence of hypnozoites causing relapses and safety concerns with primaquine in g6pd deficient individuals. we present the epidemiology of malaria with emphasis on recurrence of vivax malaria over a period of four years in southwest delhi among patients reporting to malaria clinic.201526714507
genotyping plasmodium vivax isolates infecting anopheles stephensi, an asian main malaria vector.malaria is a serious vector-borne infectious disease in iran and anopheles stephensi has long been suspected as a main malaria vector. however, opinions about its vectorial capacity in supporting plasmodium species and strains are not clear. this study investigates the susceptibility of an asian main malaria vector, an. stephensi, to plasmodium vivax isolates, collected from tropical region of iran. p. vivax gametocytes which used in ex vivo assay to an. stephensi infection were genotyped by usi ...201323384706
di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426).pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ...201525912083
application of biogenic carbon dioxide produced by yeast with different carbon sources for attraction of mosquitoes towards adult mosquito traps.surveillance is a prime requisite for controlling arthropod vectors like mosquitoes that transmit diseases such as malaria, dengue and chikungunya. carbon dioxide (co2) is one of the main cues from vertebrate breath that attracts mosquitoes towards the host. hence, co2 is used as an attractant during surveillance of mosquitoes either from commercial cylinders or dry ice for mosquito traps. in the present study, the biogenic carbon dioxide production was optimized with different carbon sources su ...201626677098
mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action.mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ...201526267052
plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methicillin resistant staphylococcus aureus biofilm and blood sucking mosquito larvae.in this study, zinc oxide nanoparticles were biologically synthesized using the leaf extract of plectranthus amboinicus (pam-zno nps). the synthesized pam-zno nps were characterized by uv-vis spectrophotometer, ftir, tem and xrd analysis. tem analysis of pam-zno nps showed the average size of about 20-50 nm. pam-zno nps control the growth of methicillin-resistant staphylococcus aureus biofilms (mrsa atcc 33591) at the concentration of 8-10 μg/ml. confocal laser scanning microscope (clsm) images ...201525280336
lethal effect of streptomyces citreofluorescens against larvae of malaria, filaria and dengue vectors.to investigate lethal effect of culture filtrates of streptomyces citreofluorescens (s. citreofluorescens) against anopheles stephensi (an. stephensi), culex quinquefasciatus (cx. quinquefasciatus), and aedes aegypti (ae. aegypti) larvae vectors for malaria, filarial and dengue.201222840445
characterization of a pathogen related to vavraia culicis detected in a laboratory colony of anopheles stephensi. 200617169070
ovicidal and adulticidal potential of leaf and seed extract of albizia lebbeck (l.) benth. (family: fabaceae) against culex quinquefasciatus, aedes aegypti, and anopheles stephensi (diptera: culicidae).several diseases are associated to the mosquito-human interaction. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. in the present study, hexane, benzene, chloroform, ethyl acetate, and methanol extracts of leaf and seed of albizia lebbeck were assayed for their toxicity agai ...201525681143
laboratory evaluation of indian medicinal plants as repellents against malaria, dengue, and filariasis vector mosquitoes.mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world ...201525399815
ovicidal, larvicidal and adulticidal properties of asparagus racemosus (willd.) (family: asparagaceae) root extracts against filariasis (culex quinquefasciatus), dengue (aedes aegypti) and malaria (anopheles stephensi) vector mosquitoes (diptera: culicidae).several diseases are associated to the mosquito-human interaction. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. the present investigation was undertaken to study the ovicidal, larvicidal and adulticidal activities of crude hexane, ethyl acetate, benzene, chloroform and met ...201424488078
green synthesis of silver nanoparticles using sida acuta (malvaceae) leaf extract against culex quinquefasciatus, anopheles stephensi, and aedes aegypti (diptera: culicidae).mosquitoes act as a vector for most of the life-threatening diseases like malaria, yellow fever, dengue fever, chikungunya fever, filariasis, encephalitis, west nile virus infection, etc. under the integrated mosquito management, emphasis was given on the application of alternative strategies in mosquito control. the continuous application of synthetic insecticides causes development of resistance in vector species, biological magnification of toxic substances through the food chain, and adverse ...201324005479
larvicidal, pupicidal, repellent and adulticidal activity of citrus sinensis orange peel extract against anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikunguniya fever, west nile virus and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. the present study explored the effects of orange peel ethanol extract of citrus sinensis on larvicidal, pupicidal, repellent and adulticidal activity against anopheles stephensi, aedes aegypti and culex quinquefasciatus. the or ...201222797605
boswellia ovalifoliolata (burseraceae) essential oil as an eco-friendly larvicide? toxicity against six mosquito vectors of public health importance, non-target mosquito fishes, backswimmers, and water bugs.the use of synthetic pesticides to control vector populations is detrimental to human health and the environment and may lead to the development of resistant strains. plants can be alternative sources of safer compounds effective on mosquito vectors. in this study, the mosquito larvicidal activity of boswellia ovalifoliolata leaf essential oil (eo) was evaluated against anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. ...201728332087
vector competence of anopheles and culex mosquitoes for zika virus.zika virus is a newly emergent mosquito-borne flavivirus that has caused recent large outbreaks in the new world, leading to dramatic increases in serious disease pathology including guillain-barre syndrome, newborn microcephaly, and infant brain damage. although aedes mosquitoes are thought to be the primary mosquito species driving infection, the virus has been isolated from dozens of mosquito species, including culex and anopheles species, and we lack a thorough understanding of which mosquit ...201728316896
larvicidal activity of blumea eriantha essential oil and its components against six mosquito species, including zika virus vectors: the promising potential of (4e,6z)-allo-ocimene, carvotanacetone and dodecyl acetate.the effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. this is due to the fact that some invasive mosquitoes, with special reference to the aedes genus, are particularly difficult to control, due to their high ecological plasticity. moreover, the indiscriminate overuse of synthetic insecticides resulted in undesirable effects on human health and non-target organisms, as well as resistance development in targeted vectors. here, the leaf essenti ...201728168560
towards green oviposition deterrents? effectiveness of syzygium lanceolatum (myrtaceae) essential oil against six mosquito vectors and impact on four aquatic biological control agents.essential oils (eos) from plants may be alternative sources of molecules toxic against mosquito vectors of public health relevance. most of researches in this field focused on eos as larvicides or ovicides, while limited efforts focused on the exploitation of eos as oviposition deterrents. in the present study, the larvicidal and oviposition deterrent activity of syzygium lanceolatum leaf eo was evaluated against six mosquito species, anopheles stephensi, an. subpictus, aedes aegypti, ae. albopi ...201627921244
toxicity of ar-curcumene and epi-β-bisabolol from hedychium larsenii (zingiberaceae) essential oil on malaria, chikungunya and st. louis encephalitis mosquito vectors.mosquitoes act as vectors of key pathogens and parasites. plant essential oils have been recognized as important sources of biopesticides, which do not induce resistance and have limited toxic effects on human health and non-target organisms. in this research, we evaluated the larvicidal and oviposition deterrence activity of hedychium larsenii essential oil (eo) and its major compounds ar-curcumene and epi-β-bisabolol. both molecules showed high toxicity against early third instars of anopheles ...201727918946
single-step biological fabrication of colloidal silver nanoparticles using hugonia mystax: larvicidal potential against zika virus, dengue, and malaria vector mosquitoes.mosquito control is facing key challenges, including outbreaks of new arbovirus threats. we proposed an eco-friendly synthesis of silver nanoparticles (agnps) employing a low-cost extract of hugonia mystax. agnps were specified by uv, xrd, ftir and edx spectroscopy, sem and tem. agnps were more toxic to anopheles stephensi, aedes aegypti, and culex quinquefasciatus larvae (lc50: 14.45, 15.86, and 17.46 μg/ml) if compared to aquatic biocontrol organisms gambusia affinis, diplonychus indicus, and ...201627611658
acute toxicity and repellent activity of the origanum scabrum boiss. & heldr. (lamiaceae) essential oil against four mosquito vectors of public health importance and its biosafety on non-target aquatic organisms.the recent outbreaks of dengue, chikungunya, and zika virus highlighted the pivotal importance of mosquito vector control in tropical and subtropical areas worldwide. however, mosquito control is facing hot challenges, mainly due to the rapid development of pesticide resistance in culicidae and the limited success of biocontrol programs on aedes mosquitoes. in this framework, screening botanicals for their mosquitocidal potential may offer effective and eco-friendly tools in the fight against mo ...201627604128
one-pot fabrication of silver nanocrystals using nicandra physalodes: a novel route for mosquito vector control with moderate toxicity on non-target water bugs.mosquitoes (diptera: culicidae) as vectors for important diseases and parasites causing millions of deaths every year. the use of synthetic pesticides against culicidae leads to resistance and environmental concerns. therefore, eco-friendly control tools are a priority. in this research, nicandra physalodes-mediated synthesis of silver nanoparticles (ag nps) was conducted, in order to control larval populations of three important mosquito vectors, anopheles stephensi, aedes aegypti and culex qui ...201627473981
one-step synthesis of polydispersed silver nanocrystals using malva sylvestris: an eco-friendly mosquito larvicide with negligible impact on non-target aquatic organisms.the synthesis of eco-friendly nanoparticles is evergreen branch of nanoscience with a growing number of biomedical implications. in this study, we investigated the synthesis of polydisperse and stable silver nanoparticles (agnp) using a cheap leaf extract of malva sylvestris (malvaceae). bio-reduced agnp were characterized by uv-visible spectrophotometry, fourier transform infrared spectroscopy (ftir), x-ray diffraction analysis (xrd), atomic force microscopy (afm), scanning electron microscopy ...201627075309
larvicidal and repellent properties of adansonia digitata against medically important human malarial vector mosquito anopheles stephensi (diptera: culicidae).development of plant-based alternative compounds for mosquito control has gained importance now-a-days, in view of increasing resistance in mosquito vectors to existing insecticides. the larvicidal and repellent activities of benzene, chloroform, hexane and methanol leaf extracts of indian medicinal plant, adansonia digitata were investigated against malarial vector, anopheles stephensi.201222898480
differential pathogenicity of metarhizium blastospores and conidia against larvae of three mosquito species.biorational insecticides are being increasingly used in integrated pest management programs. in laboratory bioassays, the pathogenicity of blastospores and conidia of the entomopathogenic fungus metarhizium brunneum arsef 4556 was evaluated against larvae of three mosquito species. three propagule concentrations (1 × 106, 1 × 107, and 1 × 108 spores ml - 1) were used in the bioassays. results showed that aedes aegypti had lower survival rates when exposed to blastospores than when exposed to con ...201728399202
fabrication of highly effective mosquito nanolarvicides using an asian plant of ethno-pharmacological interest, priyangu (aglaia elaeagnoidea): toxicity on non-target mosquito natural enemies.mosquitoes threaten the lives of humans, livestock, pets and wildlife around the globe, due to their ability to vector devastating diseases. aglaia elaeagnoidea, commonly known as priyangu, is widely employed in asian traditional medicine and pest control. medicinal activities include anti-inflammatory, analgesic, anticancer, and anesthetic actions. flavaglines, six cyclopenta[b]benzofurans, a cyclopenta[bc]benzopyran, a benzo[b]oxepine, and an aromatic butyrolactone showed antifungal properties ...201728390026
single molecule rna sequencing uncovers trans-splicing and improves annotations in anopheles stephensi.single molecule real-time (smrt) sequencing has recently been used to obtain full-length cdna sequences that improve genome annotation and reveal rna isoforms. here, we used one such method called isoform sequencing from pacific biosciences (pacbio) to sequence a cdna library from the asian malaria mosquito anopheles stephensi. more than 600 000 full-length cdnas, referred to as reads of insert, were identified. owing to the inherently high error rate of pacbio sequencing, we tested different ap ...201728181326
green synthesis of selenium nanoparticles conjugated clausena dentata plant leaf extract and their insecticidal potential against mosquito vectors.mosquitoes are major vectors for the transmission of many diseases like chikungunya, malaria, dengue, zika, etc. worldwide. in the present study, selenium nanoparticles (senps) were synthesized from clausena dentata and were tested for their larvicidal efficacy against the fourth-instar larvae of anopheles stephensi, aedes aegypti, and culex quinquefasciatus. the synthesized nanoparticles were characterized using uv-vis spectroscopy, fourier transform infrared radiation (ftir) spectroscopy, edax ...201627832715
artemisia absinthium-borne compounds as novel larvicides: effectiveness against six mosquito vectors and acute toxicity on non-target aquatic organisms.the eco-friendly control of mosquito vectors is a crucial challenge of public health importance. here we evaluated the larvicidal potential of artemisia absinthium essential oil (eo) and its three major chemical constituents against six mosquito vectors: anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. the eo was obtained by leaf hydro-distillation. its chemical composition was analyzed using gas chromatography-mass s ...201627630101
structural characterization and evaluation of mosquito-larvicidal property of silver nanoparticles synthesized from the seaweed, turbinaria ornata (turner) j. agardh 1848.the silver nanoparticles synthesized from turbinaria ornata (to-agnps) showed spherical with crystalline nature (20-32 nm) was evaluated against fourth instar larvae of three mosquitoes. the maximum activity of to-agnps was recorded on aedes aegypti followed by anopheles stephensi and culex quinquefasciatus with the following lethal concentration values (μg/ml): lc50 of 0.738, 1.134, and 1.494; and lc90 of 3.342, 17.982, and 22.475, respectively. the obtained respective values (μg/ml) vis-a-vis ...201627327539
δ-cadinene, calarene and .δ-4-carene from kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes.mosquitoes (diptera: culicidae) are major vectors of important pathogens and parasites. malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis cause millions of deaths every year. mosquito control is being challenging due to the development of pesticide resistance and negative environmental concerns. in this scenario, plants employed in traditional asian medicine may be alternative sources of newer and effective mosquitocides. in this research, we evaluated th ...201627151483
chemical composition, toxicity and non-target effects of pinus kesiya essential oil: an eco-friendly and novel larvicide against malaria, dengue and lymphatic filariasis mosquito vectors.mosquitoes (diptera: culicidae) are vectors of important parasites and pathogens causing death, poverty and social disability worldwide, with special reference to tropical and subtropical countries. the overuse of synthetic insecticides to control mosquito vectors lead to resistance, adverse environmental effects and high operational costs. therefore, the development of eco-friendly control tools is an important public health challenge. in this study, the mosquito larvicidal activity of pinus ke ...201626995063
larvicidal and repellent potential of zingiber nimmonii (j. graham) dalzell (zingiberaceae) essential oil: an eco-friendly tool against malaria, dengue, and lymphatic filariasis mosquito vectors?mosquitoes (diptera: culicidae) are important vectors of terms of public health relevance, especially in tropical and sub-tropical regions. the continuous and indiscriminate use of conventional pesticides for the control of mosquito vectors has resulted in the development of resistance and negative impacts on non-target organisms and the environment. therefore, there is a need for development of effective mosquito control tools. in this study, the larvicidal and repellent activity of zingiber ni ...201626792432
bioactivity and laundering resistance of five commercially available, factory-treated permethrin-impregnated fabrics for the prevention of mosquito-borne diseases: the need for a standardized testing and licensing procedure.personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. in this regard, a major advance has been the development of residual insecticides that can be impregnated into clothing. currently, however, information on specific treatment procedures, initial insecticide concentrations, arthropod toxicity, residual activity, and laundering resistance is either fragmentary or non-existent, and no world health organization pesticides ...201626738734
larvicidal activity of catechin isolated from leucas aspera against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of plant origin my serve as an alternative biocontrol technique in the future. the aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extracts of leucas aspera on the fourth-instar larvae of aedes aegypti, anopheles stephensi, and culex quinquefasciatus. the larvae were exposed to fractions with concentrations ranging ...201626711450
plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review.mosquitoes (diptera: culicidae) are a key threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. mosquito young instars are usually targeted with organophosphates, insect growth regulators and microbial control agents. indoors residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have strong negative effects on human health and the environment. newer and safer tools have been recently implemented to enh ...201626541154
spectral and hrtem analyses of annona muricata leaf extract mediated silver nanoparticles and its larvicidal efficacy against three mosquito vectors anopheles stephensi, culex quinquefasciatus, and aedes aegypti.mosquitoes transmit various diseases which mainly affect the human beings and every year cause millions of deaths globally. currently available chemical and synthetic mosquitocidal agents pose severe side effects, pollute the environment vigorously, and become resistance. there is an urgent need to identify and develop the cost effective, compatible and eco-friendly product for mosquito control. the present study was aimed to find out the larvicidal potential of aqueous crude extract and green s ...201526410042
eco-friendly control of malaria and arbovirus vectors using the mosquitofish gambusia affinis and ultra-low dosages of mimusops elengi-synthesized silver nanoparticles: towards an integrative approach?mosquito-borne diseases represent a deadly threat for millions of people worldwide. however, the use of synthetic insecticides to control culicidae may lead to high operational costs and adverse non-target effects. plant-borne compounds have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles. their impact against biological control agents of mosquito larval populations has been poorly studied. we synthesized silver nanoparticles (agnp) using the aqueous leaf extract o ...201526300364
green synthesis of silver nanoparticles from cassia roxburghii-a most potent power for mosquito control.mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, silver nanoparticles (agnps) synthesized using cassia roxburghii plant leaf extract against anopheles stephensi, aedes aegypt ...201526276645
effect of chloroxylon swietenia dc bark extracts against culex quinquefasciatus, aedes aegypti, and anopheles stephensi larvae.mosquitoes are the vector of more diseases and cause major health problems like malaria, dengue, chikungunya, and lymphatic filariasis. this article deals with the mosquito larvicidal activity of chloroxylon swietenia dc bark extracts against late third instar larvae of culex quinquefasciatus, aedes aegypti, and anopheles stephensi. methanolic crude extract of ch. swietenia bark was obtained by soxhlet apparatus and aqueous crude extract by cold percolation method. the range of concentrations of ...201526246308
forced egg retention and oviposition behavior of malaria, dengue and filariasis vectors to a topical repellent diethyl-phenylacetamide.egg retention and oviposition behavior of four species of mosquito vectors viz., anopheles stephensi, aedes aegypti, aedes albopictus, and culex quinquefasciatus to a topical insect repellent diethyl-phenylacetamide (depa) at 0.1-1000 mg/l was investigated under laboratory conditions. based on oviposition activity indices, depa demonstrated concentration dependent oviposition deterrent effect to a. stephensi (-0.18 to -0.97), a. aegypti (-0.18 to -0.91) and a. albopictus (-0.50 to -0.98) females ...201526245028
plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review.mosquitoes (diptera: culicidae) are a huge threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. culicidae control is of crucial importance. mosquito eggs, larvae, and pupae are usually targeted using organophosphates, insect growth regulators, and microbial agents. indoor residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have negative effects on human health and the environment, and induce resista ...201526239801
evaluation of silver nanoparticles toxicity of arachis hypogaea peel extracts and its larvicidal activity against malaria and dengue vectors.silver nanoparticles (agnps) were successfully synthesised from aqueous silver nitrate using the extracts of arachis hypogaea peels. the synthesised snps were characterized by fourier transform-infrared spectroscopy analysis, x-ray diffraction, transmission electron microscopy analysis and high-resonance scanning electron microscopy, and energy dispersive x-ray spectroscopy. agnps were well defined and measured 20 to 50 nm in size. the nanoparticles were crystallized with a face-centered cubic s ...201526154036
insecticidal potential of ocimum canum plant extracts against anopheles stephensi, aedes aegypti and culex quinquefasciatus larval and adult mosquitoes (diptera: culicidae).mosquitoes have developed resistance to various synthetic insecticides, making their control increasingly difficult. insecticides of botanical origin may serve as suitable natural control. this study evaluates the toxic potential of ocimum canum (sims) leaf extract and powder against anopheles stephensi (liston), aedes aegypti (lin) and culex quinquefasciatus (say) larval and adult mosquitoes. larval mortality was observed after 24 h recovery period and adult smoke toxicity observed for 40 min d ...201626135241
proteomic changes occurring in the malaria mosquitoes anopheles gambiae and anopheles stephensi during aging.the age of mosquitoes is a crucial determinant of their ability to transmit pathogens and their resistance to insecticides. we investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes anopheles gambiae and anopheles stephensi as they aged. protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption i ...201526100052
phytochemical profile and larvicidal properties of seed essential oil from nigella sativa l. (ranunculaceae), against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).the present study deals with investigation of larvicidal activity and their chemical constituents of the essential oil from the seeds of nigella sativa l. (ranunculaceae). totally, 18 chemical compounds were identified by gc and gc-ms analysis. thymol (19.13 %) and α-phellandrene (14.9 %) were identified as major chemical components followed by camphor (12.14 %), borneol (11.31 %), and carvacrol (8.65 %). the larval mortality was observed after 12 and 24 h of exposure period. the results reveale ...201526091760
annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector.mosquitoes transmit several diseases which cause millions of deaths every year. the use of synthetic insecticides to control mosquitoes caused diverse effects to the environment, mammals, and high manufacturing cost. the present study was aimed to test the larvicidal activity of green synthesized silver nanoparticles using annona muricata plant leaf extract against third instar larvae of three medically important mosquitoes, i.e., aedes aegypti, anopheles stephensi, and culex quinquefasciatus. t ...201526002825
gc-ms analysis of bioactive components and synthesis of gold nanoparticle using chloroxylon swietenia dc leaf extract and its larvicidal activity.a rapid bio-reduction of chloroauric acid (haucl4) was achieved by chloroxylon swietenia dc leaf extract (csle), which resulted in the formation of well dispersed c. swietenia gold nanoparticles (csgnps). the formation of gnps was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. the characterization of synthesized csgnps was made through x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), high resolution transmission electron ...201525854160
cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod mesocyclops aspericornis against malaria and dengue mosquitoes.plant-borne compounds can be employed to synthesize mosquitocidal nanoparticles that are effective at low doses. however, how they affect the activity of mosquito predators in the aquatic environment is unknown. in this study, we synthesized gold nanoparticles (aun) using the leaf extract of cymbopogon citratus, which acted as a reducing and capping agent. aun were characterized by a variety of biophysical methods and sorted for size in order to confirm structural integrity. c. citratus extract ...201525819295
an investigation on the diversity of mosquitocidal bacteria and its relationship with incidence of vector borne diseases.control of mosquitoes is the most important aspect of public health, as mosquitoes transmit many human diseases, including the fatal infection, japanese encephalitis. this paper addresses the isolation of new mosquitocidal bacteria from soil samples in the union territory of pondicherry, india, where, no clinical cases of vector borne infections have been reported. bacterial isolates from soil samples were screened for potential mosquitocidal strains and bioassays against mosquito vectors (culex ...201525801257
feeding stage, species, body part and sex-specific activity of glutathione s-transferase in mosquito.in the present study, the feeding stage, body parts, development and sex specific activity of glutathione s-transferases (gsts) were observed in different mosquito species (aedes aegypti, culex quinquefasciatus, anopheles stephensi, an. culicifacies, an. annularis, an. subpictus, an. vagus). gst activity was assayed spectrophotometrically at 23°c, using a uv max microplate reader, to measure the rate of conjugation of gsh to cdnb. a significant species-specific difference in the activity of gst ...201525801255
identification of chemical constituents and larvicidal activity of essential oil from murraya exotica l. (rutaceae) against aedes aegypti, anopheles stephensi and culex quinquefasciatus (diptera: culicidae).this study was conducted to evaluate the phytochemical composition and larvicidal effect of leaf essential oil from murraya exotica against early fourth-instar larvae of aedes aegypti, anopheles stephensi and culex quinquefasciatus. gas chromatography (gc) and gas chromatography mass spectrometry (gc-ms) analyses revealed that the essential oil contained 27 components. the major chemical components identified were β-humulene (40.62%), benzyl benzoate (23.96%), β-caryophyllene (7.05%) and α-terpi ...201525697880
synthesis and characterization of silver nanoparticles using gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes.mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. the continued use of synthetic insecticides has resulted in resistance in mosquitoes. synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to envir ...201525666372
evaluation of some aromatic plant extracts for mosquito larvicidal potential against culex quinquefasciatus, aedes aegypti, and anopheles stephensi.in the present investigation, larvicidal potential of hexane, choloroform, ethyl acetate, acetone, and methanol extracts of seven aromatic plants, viz., blumea mollis, chloroxylon swietenia, clausena anisata, feronia limnonia, lantana camera, plectranthus amboinicus, and tagetes erecta were screened against culex quinquefasciatus, aedes aegypti, and anopheles stephensi. the larval mortality was observed after 12 and 24 h of exposure period. the results revealed that all the extracts showed varie ...201525630696
larvicidal and repellent properties of streptomyces sp. vitjs4 crude extract against anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).the aim of the present study was to assess the larvicidal and repellent properties of marine streptomyces sp. vitjs4 crude extracts. the marine soil samples were collected from the puducherry coast, tamil nadu, india. the isolate streptomyces sp. vitjs4 was taxonomically characterized and identified. the ethyl acetate crude extract tested for larvicidal property showed 100% mortality for all the 3 species after 24 h exposure against the early fourth instar larvae of malarial vector--anopheles st ...201425546945
mosquito larvicidal potential of silver nanoparticles synthesized using chomelia asiatica (rubiaceae) against anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae).mosquitoes transmit serious human diseases, causing millions of deaths every year. mosquito control is to enhance the health and quality of life of county residents and visitors through the reduction of mosquito populations. mosquito control is a serious concern in developing countries like india due to the lack of general awareness, development of resistance, and socioeconomic reasons. today, nanotechnology is a promising research domain which has a wide ranging application in vector control pr ...201525544703
detection and isolation of the α-proteobacterium asaia in culex mosquitoes.investigations of microbiota within mosquitoes continue to widen the spectrum of possible symbiont-based applications against vector-borne diseases. in this context, α-proteobacteria of the genus asaia (rhodospirillales: acetobacteraceae) are emerging as possible endosymbiotic candidates, particularly in paratransgenic approaches aimed at interrupting pathogen transmission. previous studies have shown that asaia spp. distribution among anopheles gambiae and anopheles stephensi (diptera: culicida ...201425387864
adulticidal and smoke toxicity of cipadessa baccifera (roth) plant extracts against anopheles stephensi, aedes aegypti, and culex quinquefasciatus.mosquito vectors are responsible for the transmission of parasitic and viral infections, including loss in commercial and labor outputs, particularly in developing countries with tropical and subtropical climates. the aim of the present study is to evaluate the adulticidal and smoke toxicity of cipadessa baccifera (roth) against three important mosquitoes vectors, anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae). adult mortality was observed after 24-h recovery ...201525320044
screening of rubiaceae and apocynaceae extracts for mosquito larvicidal potential.rubiaceae and apocynaceae families are well known for the expression of cyclotides having insecticidal properties. leaves and flowers extracts of plants from the families rubiaceae (ixora coccinea) and apocynaceae (allamanda violacea) were evaluated for mosquito larvicidal effect against early ivth instars of aedes aegypti and anopheles stephensi. two forms of plant extracts, one untreated and the other treated with heat and proteolytic enzyme were used for assay. after primary assay, the extrac ...201525317964
evaluation of plant-mediated synthesized silver nanoparticles against vector mosquitoes.diseases transmitted by blood-feeding mosquitoes, such as dengue fever, dengue hemorrhagic fever, japanese encephalitis, malaria, and filariasis, are increasing in prevalence, particularly in tropical and subtropical zones. to control mosquitoes and mosquito-borne diseases, which have worldwide health and economic impacts, synthetic insecticide-based interventions are still necessary, particularly in situations of epidemic outbreak and sudden increases of adult mosquitoes. green nanoparticle syn ...201425300419
adulticidal properties of synthesized silver nanoparticles using leaf extracts of feronia elephantum (rutaceae) against filariasis, malaria, and dengue vector mosquitoes.mosquito-borne diseases with an economic impact create loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates. mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. extracts from plants may be alternative sources of mosquito control agents because they constitute a rich source of bioactive compounds that are biodegradable into nontoxic products and potentially suitable for use to control mosquitoes. i ...201425146645
standardization of a bottle assay--an indigenous method for laboratory and field monitoring of insecticide resistance and comparison with who adult susceptibility test.the who adult susceptibility test is in use for insecticide resistance monitoring. presently, materials are being imported from the universiti sains malaysia, malaysia and sometimes it is cost prohibitive. as an alternative, we present here a method of bottle bioassay using indigenous material. different aspects related to the assay were studied and validated in the field. bottle assay was standardized in the laboratory by using locally sourced material and laboratory-maintained insecticide-susc ...201425098343
biofabrication of ag nanoparticles using sterculia foetida l. seed extract and their toxic potential against mosquito vectors and hela cancer cells.a one-step and eco-friendly process for the synthesis of silver-(protein-lipid) nanoparticles (ag-pl nps) (core-shell) has been developed using the seed extract from wild indian almond tree, sterculia foetida (l.) (sterculiaceae). the reaction temperature played a major role in controlling the size and shell formation of nps. the amount of nps synthesized and qualitative characterization was done by uv-vis spectroscopy and transmission electron microscopy (tem), respectively. tem studies exhibit ...201424863217
chemical composition and larvicidal activity of plant extracts from clausena dentata (willd) (rutaceae) against dengue, malaria, and filariasis vectors.mosquitoes in the larval stage are attractive targets for pesticides because mosquitoes breed in water, and thus, it is easy to deal with them in this habitat. the use of conventional pesticides in the water sources, however, introduces many risks to people and/or the environment. natural pesticides, especially those derived from plants, are more promising in this aspect. aromatic plants and their essential oils are very important sources of many compounds that are used in different respects. in ...201424802866
mosquito larvicidal properties of silver nanoparticles synthesized using heliotropium indicum (boraginaceae) against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).mosquitoes transmit dreadful diseases to human beings wherein biological control of these vectors using plant-derived molecules would be an alternative to reduce mosquito population. in the present study activity of aqueous leaf extract and silver nanoparticles (agnps) synthesized using helitropium indicum plant leaves against late third instar larvae of aedes aegypti, anopheles stephensi and culex quinquefasciatus. the range of varying concentrations of synthesized agnps (8, 16, 24, 32, and 40  ...201424770671
larvicidal potential of wild mustard (cleome viscosa) and gokhru (tribulus terrestris) against mosquito vectors in the semi-arid region of western rajasthan.cleome viscosa l. (family: capparaceae) commonly known as tickweed or wild mustard and tribulus terrestris l. (family: zygophyllaceae) commonly known as gokhru, growing wildly in the desert areas in the monsoon and post monsoon season, are of great medicinal importance. comparative larvicidal efficacy of the extracts from seeds of c. viscosa and fruits and leaves of t. terrestris was evaluated against 3rd or early 4th stage larvae of anopheles stephensi (liston), aedes aegypti (linnaeus) and cul ...201424665757
low-cost and eco-friendly green synthesis of silver nanoparticles using feronia elephantum (rutaceae) against culex quinquefasciatus, anopheles stephensi, and aedes aegypti (diptera: culicidae).mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, the larvicidal activity of silver nanoparticles (agnps) synthesized using feronia elephantum plant leaf extract against late ...201424647984
larvicidal, ovicidal, and oviposition-deterrent activities of four plant extracts against three mosquito species.in mosquito control programs, insecticides of botanical origin have the potential to eliminate eggs, larvae, and adults. so, the larvicidal, ovicidal, and oviposition-deterrent activities of petroleum ether and ethyl acetate extracts of the leaves of eugenia jambolana, solidago canadensis, euodia ridleyi, and spilanthes mauritiana were assayed against the three vector mosquito species, namely anopheles stephensi, aedes aegypti, and culex quinquefasciatus. the larval bioassay was conducted follow ...201424562451
larvicidal, ovicidal, and adulticidal efficacy of erythrina indica (lam.) (family: fabaceae) against anopheles stephensi, aedes aegypti, and culex quinquefasciatus (diptera: culicidae).mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis, and japanese encephalitis. mosquito control is facing a threat because of the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. in view of the recently increased interest in developing plant origin insecticides as an alternative to chemical insecticide, this study was unde ...201424322290
larvicidal activity and gc-ms analysis of flavonoids of vitex negundo and andrographis paniculata against two vector mosquitoes anopheles stephensi and aedes aegypti.development of insect resistance to synthetic pesticides, high operational cost and environmental pollution have created the need for developing alternative approaches to control vector-borne diseases. in the present study, larvicidal activity of flavonoid extracts of different parts of vitex negundo (linnaeus) and andrographis paniculata (nees) have been studied against the late iii or early iv instar larvae of aedes aegypti and anopheles stephensi (liston).201324220075
strong larvicidal potential of artemisia annua leaf extract against malaria (anopheles stephensi liston) and dengue (aedes aegypti l.) vectors and bioassay-driven isolation of the marker compounds.malaria and dengue are the two most important vector-borne human diseases caused by mosquito vectors anopheles stephensi and aedes aegypti, respectively. of the various strategies adopted for eliminating these diseases, controlling of vectors through herbs has been reckoned as one of the important measures for preventing their resurgence. artemisia annua leaf chloroform extract when tried against larvae of a. stephensi and a. aegypti has shown a strong larvicidal activity against both of these v ...201424158647
mosquitocidal properties of solanum trilobatum l. (solanaceae) leaf extracts against three important human vector mosquitoes (diptera: culicidae).to determine the larvicidal and pupicidal activites of solanum trilobatum (s. trilobatum) leaf extracts against aedes aegypti (ae. aegypti), culex quinquefasciatus (cx. quinquefasciatus) and anopheles stephensi (an. stephensi).201324083579
mosquito larvicidal and ovicidal activity of puffer fish extracts against anopheles stephensi, culex quinquefasciatus and aedes aegypti (diptera: culicidae).the extracts of liver (le), ovary (oe), skin (se) and muscle (me) tissues of four species of puffer fishes viz., arothron hispidus, lagocephalus inermis, lagocephalus scleratus and chelonodon patoca were evaluated against larvae and eggs of three mosquito vectors, anopheles stephensi, culex quinquefasciatus and aedes aegypti. the lc50 values were 1194.26, 1382.73 (le); 1421.42, 1982.73 (oe); 7116.86, 15038.98 (me) and 10817.8 ppm (se) for an. stephensi and cx. quinquefasciatus respectively for a ...201323665705
green synthesis of silver nanoparticles using murraya koenigii leaf extract against anopheles stephensi and aedes aegypti.mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, the activity of silver nanoparticles (agnps) synthesized using murraya koenigii plant leaf extract against first to fourth in ...201323322327
ovicidal, repellent, adulticidal and field evaluations of plant extract against dengue, malaria and filarial vectors.mosquitoes are insect vectors responsible for the transmission of parasitic and viral infections to millions of people worldwide, with substantial morbidity and mortality. infections transmitted by mosquitoes include malaria, yellow fever, chikungunya, filariasis and other arboviruses. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. the adulticidal activities of crude hexane, benzene, ethyl acetate, acetone and methanol leaf extracts of aca ...201323271569
integration of botanical and bacterial insecticide against aedes aegypti and anopheles stephensi.the present study evaluated the orthosiphon thymiflorus leaf extract and the bacterial insecticide spinosad, testing the first to fourth instars larvae and pupae of two important vector mosquitoes, viz., aedes aegypti, anopheles stephensi. the fresh leaves of o. thymiflorus were washed thoroughly in tap water and shade-dried at room temperature (28 ± 2 °c) for 5 to 8 days. the air-dried materials were powdered separately using a commercial electrical blender. from the plants, 500 g powder was ma ...201323242266
chemical constituents and larvicidal potential of feronia limonia leaf essential oil against anopheles stephensi, aedes aegypti and culex quinquefasciatus.in the present investigation, the leaf essential oil of feronia limonia was evaluated for chemical constituents and mosquito larvicidal activity against the larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus. gc and gc-ms analyses revealed that the essential oil contain 51 compounds. estragole (34.69 %) and β-pinene(23.59 %) were identified as the major constituents followed by methyl (z)-caryophyllene (11.05 %), eugenol (6.50 %), linalool (3.97 %), phytol (3.27 %), sabinene ...201323160893
laboratory observations on the larvicidal efficacy of three plant species against mosquito vectors of malaria, dengue/dengue hemorrhagic fever (df/dhf) and lymphatic filariasis in the semi-arid desert.comparative larvicidal efficacy of aqueous and organic solvent extracts from seeds, leaves and flowers of three desert plants viz. calotropis procera (aiton), tephrosia purpurea (l.) pers. and prosopis juliflora (sw.) dc. was evaluated against anopheles stephensi (liston), aedes aegypti (linnaeus) and culex quinquefasciatus (say). for this purpose larvae of all the three mosquito species were reared in the laboratory and studies carried out on late 3rd or early 4th instars using standard who tec ...201223029912
adulticidal properties of cardiospermum halicacabum plant extracts against three important vector mosquitoes.to determine adulticidal activitiy of hexane, ethyl acetate, benzene, chloroform and methanol leaf extracts of cardiospermum halicacabum against culex quinquefasciatus (cx. quinquefasciatus), aedes aegypti (ae. aegypti) and anopheles stephensi (an. stephensi).201222957423
insecticide susceptibility status in three medically important species of mosquitoes, anopheles stephensi, aedes aegypti and culex quinquefasciatus, from bruhat bengaluru mahanagara palike, karnataka, india.development of resistance among mosquito vectors of diseases is of growing concern in many countries of the subtropics, including much of india. the distributions of susceptibility/resistance to insecticides are scarcely recorded and are unknown in many parts of india. this manuscript reports the susceptibility status among strains of anopheles stephensi, aedes aegypti and culex quinquefasciatus collected from different geographical areas of bruhat bengaluru mahanagara palike (bbmp), karnataka, ...201322926921
effect of ageratum houstonianum mill. (asteraceae) leaf extracts on the oviposition activity of anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).plant extracts have been studied extensively for their insecticidal activity against immature stages and adult mosquitoes. they have also been reported to influence the habitat preference of ovipositing mosquitoes. ageratum houstonianum, a medicinal plant belonging to the family asteraceae, has been reported to possess insecticidal activity, and in the present study, the ovipositional attractant/deterrent activity was studied. the effect of ageratum houstonianum crude leaf extracts on the ovipos ...201222903449
mosquito larvicidal, pupicidal, adulticidal, and repellent activity of artemisia nilagirica (family: compositae) against anopheles stephensi and aedes aegypti.mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. the aim of the present study, to evaluate the larvicidal, pupicidal, repellent, and adulticidal activities of methanol crude extract of artemisia nilagirica were assayed for their toxicity against two important vector mosquitoes, viz., anopheles stephensi and aedes aegyp ...201222903417
adulticidal and repellent properties of cassia tora linn. (family: caesalpinaceae) against culex quinquefasciatus, aedes aegypti, and anopheles stephensi.mosquitoes have developed resistance to various synthetic insecticides, making its control increasingly difficult. insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. the adulticidal and repellent activities of crude hexane, chloroform, benzene, acetone, and methanol extracts of the leaf of cassia tora were assayed for their toxicity against three important vector mosquitoes, viz., culex quinquefasciatus, aedes aegypti, and anopheles stephensi. ...201222821231
electroantennogram, flight orientation and oviposition responses of anopheles stephensi and aedes aegypti to a fatty acid ester-propyl octadecanoate.studies were carried out to evaluate the role of a c(21)-fatty acid ester; propyl octadecanoate (po) for olfaction-mediated behavioral responses of urban malaria vector, anopheles stephensi and dengue vector, aedes aegypti mosquitoes using electroantennogram (eag), flight orientation and oviposition experiments. dose dependent electrophysiological responses were recorded for po from the antenna of both mosquito species in which 10(-5) g elicited significant eag response. an. stephensi exhibited ...201222750483
larvicidal activity of morinda citrifolia l. (noni) (family: rubiaceae) leaf extract against anopheles stephensi, culex quinquefasciatus, and aedes aegypti.morinda citrifolia leaf extract was tested for larvicidal activity against three medically important mosquito vectors such as malarial vector anopheles stephensi, dengue vector aedes aegypti, and filarial vector culex quinquefasciatus. the plant material was shade dried at room temperature and powdered coarsely. from the leaf, 1-kg powder was macerated with 3.0 l of hexane, chloroform, acetone, methanol, and water sequentially for a period of 72 h each and filtered. the yield of extracts was hex ...201222740293
evaluation of certain insecticides on nettings for their efficacy and wash resistance against mosquito species.five insecticides (bifenthrin, deltamethrin, etofenprox, permethrin and lamda cyhalothrin) recommended by who, at their recommended dose were compared for their efficacy and wash resistance through bioassay against mosquito vectors, culex quinquefasciatus, aedes aegypti and anopheles stephensi. etofenprox treated nettings exhibited better knockdown and mortality than the other insecticides. the order of efficacy of the insecticides treated nettings was etofenprox > or = deltamethrin > lambda cyh ...201222734256
silica nanoparticle: a potential new insecticide for mosquito vector control.presently, there is a need for increased efforts to develop newer and effective methods to control mosquito vectors as the existing chemical and biological methods are not as effective as in earlier period owing to different technical and operational reasons. the use of nanomaterial products in various sectors of science including health increased during the last decade. we tested three types of nanosilica, namely lipophilic, hydrophilic and hydrophobic, to assess their larvicidal, pupicidal and ...201222565400
mosquito larvicidal properties of orthosiphon thymiflorus (roth) sleesen. (family: labiatae) against mosquito vectors, anopheles stephensi, culex quinquefasciatus and aedes aegypti (diptera: culicidae).to determine the mosquito larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol leaf extract of orthosiphon thymiflorus (o. thymiflorus) against anopheles stephensi (an. stephensi), culex quinquefasciatus (cx. quinquefasciatus) and aedes aegypti (ae. aegypti).201222449522
larvicidal activity of silver nanoparticles synthesized using pergularia daemia plant latex against aedes aegypti and anopheles stephensi and nontarget fish poecillia reticulata.in present study, the bioactivity of latex-producing plant pergularia daemia as well as synthesized silver nanoparticles (agnps) against the larval instars of aedes aegypti and anopheles stephensi mosquito larvae was determined. the range of concentrations of plant latex (1,000, 500, 250, 125, 62.25, and 31.25 ppm) and agnps (10, 5, 2.5, 1.25, 0.625, and 0.3125 ppm) were prepared. the lc(50) and lc(90) values for first, second, third, and fourth instars of synthesized agnps-treated first, second ...201222371271
geographical distribution and evaluation of mosquito larvivorous potential of aphanius dispar (rüppell), a native fish of gujarat, india.certain indigenous fish can play potential role in vector control. the study recorded distribution of aphanius dispar (rüppell) in its native habitats in gujarat, india and evaluated its larval propensity for indian mosquito vectors.201122297287
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