| is there a unique episome in ebv transformed b cells? | we have examined the frequency of episome formation in resting b cells 36 hr after infection with epstein-barr virus. we have detected an average of 0.65 episomal genomes per cell (sd 0.17). we have used this information to predict the distribution of circularization events in b cell clones derived from such infections. we have observed that in 32 out of 33 cases (with one unresolved) the b cell clone has arisen as a consequence of a single circularization event. this result is highly statistica ... | 1991 | 1660206 |
| early events in epstein-barr virus genome expression after activation: regulation by second messengers of b cell activation. | rna transcription from the bamhi z and bamhi r and hindiii g regions of the epstein-barr virus (ebv) genome was studied after treatment of akata cells with anti-immunoglobulin g (igg), with second messenger agonists or antagonists to determine how latent ebv activation is regulated by b cell second messengers. northern gel analysis demonstrated that bzlf1, bzlf1 + brlf1, and bmlf1 + bslf2 transcripts were induced at 2 hr and increased in concentration at 4 hr after induction with anti-igg; trans ... | 1991 | 1660209 |
| [importance and diagnosis of epstein-barr virus infection]. | the 103 serum samples have been examined for the presence of the specific antibodies against epstein-barr virus (ebv): igg and igm antibodies to viral capsid antigen (vca), antibodies to nuclear antigen (ebna) and antibodies to early antigen (ea). sixty nine studied sera belonged to patients with suspected infective mononucleosis (im) while other sera were sent with the diagnosis which could be associated with ebv infections. the results obtained have suggested the necessity of examinations of e ... | 1991 | 1660212 |
| hiv-associated lymphoma: histopathology and association with epstein-barr virus genome related to clinical, immunological and prognostic features. | all 51 cases of hiv-related malignant lymphoma in denmark diagnosed from 1983 to 1989 were reviewed. there were 12 burkitt-type lymphomas, 30 immunoblast-rich lymphomas and 9 other lymphomas. patients with immunoblast-rich lymphomas had significantly lower cd4 cell counts (median 60 vs. 188 x 10(6)/l, p less than 0.05), and more often a history of previous aids-defining illnesses (50% vs. 0%, p less than 0.005), compared with patients with burkitt-type lymphomas. epstein-barr virus (ebv) dna was ... | 1991 | 1660293 |
| organ specificity of the antigens reacting with the 48-1 and s-1 antibodies in chimpanzees infected with hepatitis c virus. | 48-1 and s-1 antibodies produced by lymphoblastoid cells transformed with epstein-barr virus were reported to be associated with infection by not only the hepatitis non-a, non-b (nanb) virus but also hepatitis delta virus. appearance of the antigens reacting with these antibodies in the liver of chimpanzees was recently found to be a host response to alpha-interferon induced by infections of both viruses. to investigate organ specificity of these antigens, various organs obtained from chimpanzee ... | 1991 | 1660298 |
| recurrent mucocutaneous lymphocytic infiltration possibly associated with epstein-barr virus infection. | a 32-year-old man had a history of recurrent but self-healing necrotizing nodules on the face and oral mucosa. histologically the lesion was composed of necrotizing foci surrounded with dense cd8+ t lymphocytic infiltration in the dermis. laboratory examination showed marked blood cd8+ t lymphocytosis with a reversed ratio of cd4+/cd8+ of 0.11. except for a very high titer of antibodies against epstein-barr-virus-associated antigens, no signs of infection were observed. | 1991 | 1660415 |
| hodgkin's disease: update of findings. | the authors critically review the problem of hodgkin's disease (hd) in the light of new morphological, immunohistochemical, kinetic, genotypic, and virological findings. these support the lymphoid origin of neoplastic hodgkin's and reed-sternberg cells, because of regular expression of the cd30 lymphoid activation antigen and frequent detection of b- or t-cell phenotypic and/or genotypic markers. it is possible to hypothesize the release of cytokines by tumoral elements as well as the presence o ... | 1991 | 1660436 |
| quantitation of t cell depletion by limiting dilution analysis. | we evaluated a culture method for enumeration of residual t cells remaining in marrow after treatment with antibody and complement or with immunotoxin. | 1991 | 1660438 |
| determination of the structural basis for selective binding of epstein-barr virus to human complement receptor type 2. | epstein-barr virus (ebv) is an oncogenic herpesvirus that selectively infects and immortalizes human b lymphocytes. one determinant of this narrow tropism is human cr2, the only viral receptor within the superfamily of proteins that contain short consensus repeats (scrs). human cr2 serves as a receptor for both c3dg and the gp350/220 glycoprotein of ebv, and binds the monoclonal antibody (mab) okb7, which blocks binding of both ligands to the receptor. in contrast, although murine cr2 is capable ... | 1991 | 1660522 |
| molecular characteristics of antibodies bearing an anti-dna-associated idiotype. | anti-double-stranded dna antibodies are the hallmark of the disease systemic lupus erythematosus and are believed to contribute to pathogenesis. while a large number of anti-dna antibodies from mice with lupus-like syndromes have been characterized and their variable region genes sequenced, few human anti-dna antibodies have been reported. we describe here the variable region gene sequences of eight antibodies produced by epstein-barr virus (ebv)-transformed b cells that bear the 3i idiotype, an ... | 1991 | 1660528 |
| the x-linked lymphoproliferative disease: from autopsy toward cloning the gene 1975-1990. | although x-linked lymphoproliferative disease (xlp) is rare (1-2 males per 1 x 10(6)), it serves as a model for discerning diverse diseases caused by epstein-barr virus (ebv) ranging from agammaglobulinemia to fatal infectious mononucleosis following infection with the virus. the study of patients with xlp has also paved the way to understanding how ebv induce diseases in children with primary immunodeficiency diseases, organ transplant recipients, and those with acquired immunodeficiency syndro ... | 1991 | 1660601 |
| multiple isomers of inositol pentakisphosphate in epstein-barr-virus- transformed (t5-1) b-lymphocytes. identification of inositol 1,3,4,5,6-pentakisphosphate, d-inositol 1,2,4,5,6-pentakisphosphate and l-inositol 1,2,4,5,6-pentakisphosphate. | substantial amounts of three [3h]insp5 isomers were detected in [3h]inositol-labelled human lymphoblastoid (t5-1) cells. their structures were determined by h.p.l.c. [phillippy & bland (1988) anal. biochem. 175, 162-166], and by utilizing a stereospecific d-inositol 1,2,4,5,6-pentakisphosphate 3-kinase from dictyostelium discoideum [stephens & irvine (1990) nature (london) 346, 580-583]. the structures were: inositol 1,3,4,5,6-pentakisphosphate, d-inositol 1,2,4,5,6-pentakisphosphate and l-inosi ... | 1991 | 1660712 |
| induction of nad(p)h:quinone reductase in human peripheral blood lymphocytes. | the induction of quinone reductase [qr; nad(p)h:(quinone acceptor) oxidoreductase; ec 1.6.99.2] in cultured cells and animal tissues of rodents has provided useful information on mechanisms of protection against carcinogens. we have developed a simple and efficient microtiter plate assay for the direct measurement of qr basal activity and inducibility in human peripheral blood lymphocytes (unstimulated, mitogen-stimulated and epstein-barr virus-transformed) grown in suspension culture. in these ... | 1991 | 1660793 |
| high-frequency transformation of human repair-deficient cell lines by an epstein-barr virus-based cdna expression vector. | we constructed a human cdna expression vector by combining an episomal epstein-barr virus (ebv) vector with the expression cassette from the transient-expression vector, pcdm8. this new vector, designated pebs7, exhibited high-level expression of reporter genes in normal and repair-deficient xeroderma pigmentosum cell lines. reconstruction experiments indicated that marker genes diluted to a frequency of 10(-5) can be rescued on a single transfection dish. moreover, derivative cell lines that co ... | 1991 | 1660831 |
| epstein-barr virus infections and hodgkin's disease: a study of fixed tissues using the polymerase chain reaction. | epstein-barr viral (ebv) infections are associated with hodgkin's disease (hd). to better characterize this relationship, fixed tissues of infectious mononucleosis, normal and reactive lymph nodes, lymph nodes with progressively transformed germinal centers, and biopsy specimens with the different subtypes of hd were analyzed by polymerase chain reaction (pcr). the presence or absence of ebv, the relative amounts of ebv, and the presence of multiple ebv genotypes as defined by amplification of a ... | 1991 | 1660852 |
| a t cell clone in association with an epstein-barr virus-related b cell lymphoma. | | 1991 | 1661038 |
| virus-associated haemophagocytic syndrome with epstein-barr virus infection. | the clinical and histological findings of a 10-year-old girl with virus-associated haemophagocytic syndrome are presented. the serum levels of epstein-barr viral antigens were elevated. epstein-barr virus (ebv) genome was detected by polymerase chain reaction in bone marrow and lymph node specimens. histologically, haemophagocytic histiocytes were present in bone marrow, and areas of non-suppurative necrosis were present in lymph nodes, where silver grain deposition of the ebv genome was demonst ... | 1991 | 1661049 |
| epstein-barr virus and hodgkin's disease. a correlative in situ hybridization and polymerase chain reaction study. | the authors studied typical hodgkin's disease along with the nodular, lymphocyte predominance subtype by both the polymerase chain reaction (pcr) and in situ hybridization for evidence of the epstein-barr virus (ebv). by pcr, ebv dna was detected in 12/23 cases of typical hodgkin's disease and 2/13 cases of the nodular, lymphocyte predominance subtype. ebv rna was detected by in situ hybridization studies within reed-sternberg cells and variants in 11/23 cases of typical hodgkin's disease and 0/ ... | 1991 | 1661073 |
| epstein-barr virus gene expression and epithelial cell differentiation in oral hairy leukoplakia. | hairy leukoplakia (hl) is an epstein-barr (eb) virus related lesion of oral mucosa that is principally associated with human immunodeficiency virus-induced immunosuppression. to understand the nature of eb virus involvement in these lesions, this study compares the distribution of eb virus dna and eb viral gene products with the pattern of keratinocyte differentiation in 12 lateral tongue biopsies of hl. evidence of replicating eb viral infection and abundant virus production was demonstrated in ... | 1991 | 1661074 |
| biology of the lymphomas: cytogenetics, molecular biology, and virology. | correlations between cytogenetics, histology, and clinical course continue to emerge in studies of non-hodgkin's lymphomas. the previously recognized association between the t(14;18) chromosomal translocation and follicular lymphoma has been confirmed; abnormalities of chromosome 3 have correlated specifically with diffuse large cell lymphoma and abnormalities of chromosome 1 have been frequently present in t-cell lymphomas. rearrangements involving 11q13 (bcl-1) occur most commonly in diffuse l ... | 1991 | 1661167 |
| pathology of malignant lymphomas. | attempts to understand the malignant lymphomas have followed two major lines of investigation: attempts to define the relationship of neoplastic lymphoid cells to stages of normal t- and b-lymphocyte differentiation, and attempts to understand the genetic abnormalities associated with malignant transformation. it is hoped that these two lines of investigation will make it possible to define clinically important entities, so that specific treatment strategies can be developed and tested, and will ... | 1991 | 1661168 |
| clinical aspects of aids-related non-hodgkin's lymphoma. | the association of malignant lymphoma with the acquired immunodeficiency syndrome (aids) has been recognized since early in the human immunodeficiency virus epidemic. important clues regarding the etiology of aids-related non-hodgkin's lymphoma (aids-nhl) and estimates of the future incidence of aids-nhl have been derived from epidemiologic studies. recent epidemiologic and cohort studies reviewed in this article have confirmed that the incidence of non-hodgkin's lymphoma is high in patients wit ... | 1991 | 1661169 |
| interleukin 10. | | 1991 | 1661188 |
| [monoclonal antibodies to rho(d)-antigen of the rhesus system. obtaining the ebv-transformed b-lymphoblastoid human cell lines secreting igg-anti-d antibodies]. | human b-lymphoblastoid cell lines producing specific anti-d antibodies against rh0(d) antigen have been established by ebv-transformation. the strong influence of the donor's immune status (serum titer of anti-d antibodies, intravenous booster injection prior to bleeding) is shown. the cell lines cloned by limiting dilution continuously produce anti-d antibodies in culture for 7 months or longer which is sufficient to support large-scale production of anti-d antibodies based on the cultivation o ... | 1991 | 1661249 |
| [human b-lymphoblastoid cell lines: optimal conditions for their procurement, selection, cultivation and cloning]. | the protocol of epstein-barr virus (ebv)-induced transformation of b-lymphocytes from reripheral blood of normal humans has been described. the requirements for mononuclear ebv-infection and for selection, cultivation, and cloning of ebv-transformed cell lines have been investigated. successful establishment of stable human immunoglobulin-producing cell lines from lymphocytes of immunized donors could be achieved following the protocol presented. | 1991 | 1661250 |
| the zta trans-activator protein stabilizes tfiid association with promoter dna by direct protein-protein interaction. | eukaryotic transcriptional activators are believed to stimulate transcription through direct and/or indirect interactions with one or more of the general transcription factors. we show here that the zta transcriptional activator protein encoded by the epstein-barr virus makes direct physical contact with the basic transcription factor tfiid. both zta and tfiid were expressed in and purified from escherichia coli. zta stabilized the binding of tfiid to zta-responsive promoters as assayed by gel e ... | 1991 | 1661258 |
| persistent polyclonal b lymphocytosis with epstein-barr virus antibodies and subsequent malignant pulmonary blastoma. | | 1991 | 1661303 |
| falsely-positive igm tests in epstein-barr virus infection. | | 1991 | 1661320 |
| frequent detection of epstein-barr viral deoxyribonucleic acid and absence of cytomegalovirus deoxyribonucleic acid in hodgkin's disease and acquired immunodeficiency syndrome-related hodgkin's disease. | epstein-barr viral dna (ebv dna) has been detected in 20 to 58% of hodgkin's disease tumors analyzed by southern blotting or polymerase chain reaction (pcr). because patients with hodgkin's disease are generally immunodepressed, it is possible that the ebv is not directly involved in the pathogenesis of hodgkin's disease but is merely detectable by molecular techniques because of reactivation of a latent infection. the purpose of this study was to determine if ebv dna could be detected in an eve ... | 1991 | 1661351 |
| immunovirology of transforming viruses. | the paradigmatic shift in cellular immunology, initiated by the realization that cytotoxic t lymphocytes recognize major histocompatibility complex class i antigens that carry endogenous (including virally encoded) protein-derived peptides in a special groove, has made a strong impact on the immunopathology of virus-associated tumors. | 1991 | 1661601 |
| posttransplant lymphoproliferative disease in thoracic organ transplant patients: ten years of cyclosporine-based immunosuppression. | lymphoproliferative disease developed in 15 heart and five lung transplant recipients during a decade of heart and lung transplantation from 1980 through 1989. the overall incidence of posttransplant lymphoproliferative disease in patients who survived more than 30 days is 4%. the incidence after heart transplantation is 3.4% and after lung transplantation is 7.9% (p = 0.08). the peak occurrence of posttransplant lymphoproliferative disease is 3 to 4 months after transplantation. however, posttr ... | 1991 | 1661607 |
| [the etiology of malignant lymphomas]. | | 1991 | 1661666 |
| [regulation of epstein-barr virus early gene expression]. | | 1991 | 1661887 |
| characterization of rhesus macaque b-lymphoblastoid cell lines infected with simian type d retrovirus. | a simian type d retrovirus designated srv induces a fatal immunosuppressive disease in rhesus macaques. this syndrome shows many clinical similarities to acquired immunodeficiency syndrome (aids) in human immunodeficiency virus-infected individuals. to investigate the mechanisms of immune dysfunction in srv infection, we have focused on the interactions of srv serotype 1 (srv-1) with macaque b-lymphoblastoid cell lines (b-lcl). procedures were optimized for establishing b-lcl by immortalization ... | 1991 | 1662056 |
| activation of o2(-)-generating oxidase in an heterologous cell-free system derived from epstein-barr-virus-transformed human b lymphocytes and bovine neutrophils. application to the study of defects in cytosolic factors in chronic granulomatous disease. | epstein-barr-virus-transformed human b lymphocytes (ebv b lymphocytes) stimulated by 4 beta-phorbol 12-myristate 13-acetate exhibit an nadph-dependent oxidase activity capable of generating the superoxide anion o2-, similar to, but less efficient than that of activated neutrophils. a cell-free system of oxidase activation consisting of a membrane fraction and cytosol from ebv b lymphocyte homogenate supplemented with guanosine 5'-[gamma-thio]triphosphate (gtp[s]), arachidonic acid and mg2+ was f ... | 1991 | 1662136 |
| cutaneous lesions of hemophagocytic syndrome in a patient with t-cell lymphoma and active epstein-barr infection. | the hemophagocytic syndrome (hps) was first described in immune-compromised patients with secondary viral infections. immunodeficiency has not always been diagnosed before the onset of hps, but most patients who develop hps have known immune deficits. hps has also been reported in association with lymphoreticular malignancies. in hps the most prominent histologic change is phagocytosis of red blood cells and other bone marrow-derived elements by cytologically benign histiocytes. considerable con ... | 1991 | 1662240 |
| the role of lactic acid in autocrine b-cell growth stimulation. | growth and survival of epstein-barr virus (ebv)-immortalized b lymphocytes cultured at low cell densities require autocrine soluble factors. in this study, we have purified a low molecular weight autocrine soluble factor that promotes growth of ebv-immortalized b cells in serum-free conditions and identified it as lactic acid (la). synthetic la stimulated growth in ebv-immortalized b cells at 1-10 mm, a concentration of la measured in the culture supernatant of ebv-immortalized cell lines. la al ... | 1991 | 1662382 |
| [experiences with monoclonal antibody orthoclone okt3 following liver transplantation]. | in 7 patients 10 cycles (5 to 21 days, dosage: 30 to 430 mg, median 138 mg) of okt3 therapy were performed after liver transplantation. in all patients reactivation of an ebv-infection with hepatitis was observed. two patients treated with high dosages (430 mg respectively 195 mg) developed lymphoproliferative lesions. in one patient with persistent ebv-infection (dosage: 360 mg) a b-cell-lymphoma was diagnosed. this patient died 26 months after transplantation. | 1991 | 1662431 |
| detection of epstein-barr virus and human herpes virus type 6 in saliva from patients with lymphoproliferative diseases by the polymerase chain reaction. | direct detection of these viruses was made by using the pcr for amplifying viral dna. in virtually all adults low levels of the herpesvirus can be detected. therefore it is necessary to quantitate the amount of viral dna, and a method to compare the plasmid containing the cloned target gene was used here. after 35 cycles of amplification, 10 copies of the viral dna per 100 microliters saliva were detected. the pcr was used to detect increased levels of ebv and hhv-6 in saliva from patients with ... | 1991 | 1662480 |
| primer terminus recognition and highly processive replication by epstein-barr virus dna polymerase. | the epstein-barr virus (ebv) dna polymerase is essential for viral dna replication in the lytic phase of the ebv life cycle. it efficiently extends rna primers on the template dna, suggesting the possible involvement of the ebv dna polymerase in synthesizing okazaki fragments from rna primers on the lagging strand template. competition experiments revealed that the ebv dna polymerase had significantly higher affinity for primer termini hybridized to the template dna than for the single-stranded ... | 1991 | 1662485 |
| cloning of human heterohybridoma cell lines using chronic lymphocytic leukemia b cells as a feeder layer. | b cells from the peripheral blood of chronic lymphocytic leukemia patients were isolated by gradient density centrifugation and used without irradiation as a feeder layer in the cloning of human heterohybridoma cell lines by limiting dilution. cloning efficiencies were high with all the cell lines tested. these feeder leukemia b cells could also be successfully used after having been stored in liquid nitrogen. | 1991 | 1662677 |
| b cell phenotype-dependent expression of the epstein-barr virus nuclear antigens ebna-2 to ebna-6: studies with somatic cell hybrids. | the expression of the transformation-associated epstein-barr virus (ebv)-encoded nuclear antigens (ebnas) 1 to 6 and membrane protein lmp-1 was studied in a series of somatic cell hybrids derived from the fusion of the ebv-transformed lymphoblastoid cell line (lcl) kr-4, and the ebv-carrying burkitt's lymphoma lines daudi, p3hr-1 and raji, with non-b cell lines of fibroblast, erythroid, myeloid and epithelial origin. expression of ebnas 2 to 6 was down-regulated in the hybrids in parallel with e ... | 1991 | 1662694 |
| epstein-barr virus infection in oral hairy leukoplakia: virus replication in the absence of a detectable latent phase. | epstein-barr virus (ebv) infects both b lymphocytes and oropharyngeal epithelium, and it has been argued that the true reservoir of virus persistence in vivo is the self-renewing basal epithelial compartment. the identification of oral hairy leukoplakia (hl) of aids patients as a clinically apparent focus of ebv replication in lingual epithelium therefore provides a means of studying the ebv-epithelial cell interaction in situ. replicative ebv dna and productive cycle antigens are restricted to ... | 1991 | 1662695 |
| cloning and characterization of cdna clones corresponding to transcripts from the bamhi g region of the epstein-barr virus genome and expression of bglf2. | a cdna library was constructed from poly(a)+ rna isolated from the iododeoxyuridine-treated p3hr1 cell line. five cdna clones, which hybridized with the bamhi g fragment of epstein-barr virus (ebv) dna, were subcloned and sequenced. clones g2, g3 and g4 corresponded to the bglf2 open reading frame (orf) of ebv (b95-8, nucleotides 126,837 to 125,866); g3 was found to contain the entire bglf2 orf. the predicted mr of the putative protein product of the ebv b95-8 bglf2 orf is 36k. complete nucleoti ... | 1991 | 1662696 |
| [evaluation of igg and iga type antibodies to capsid and early antigens of the epstein-barr virus in the diagnosis of nasopharyngeal carcinoma]. | the epstein-barr virus (ebv) is related with nasopharyngeal carcinoma (npc) in that, in this neoplasm, high levels of antibodies are found vs different ebv antigens. | 1991 | 1662737 |
| infectious etiology of rheumatoid arthritis. | the infectious etiology of rheumatoid arthritis has been a long-standing hypothesis and in recent years is being examined with greater sophistication and scientific rigor. synovitis may result indirectly from infection by the deposition of circulating immune complexes, by molecular mimicry, by in situ antigen deposition, or by arthritogenic toxins. of candidate pathogens, recent interest has focused on mycobacterial hsp, ebv, and parvovirus b19. there is circumstantial evidence to support a link ... | 1991 | 1662820 |
| epstein-barr virus activating principle in husbands' semen of cervical cancer patients. | a short-term in vitro assay for detecting tumor promoter activity has been cultivated utilizing the induction of epstein-barr virus early antigen (ebv-ea) in ebv genome-carrying human lymphoblastoid cell line (raji cell) system. by using this system, we found that some of human semen samples possessed a marked capacity to induce ebv-ea, suggesting possible tumor promotor activity of semen. in the present study, 60 semen samples obtained from husbands of cervical cancer patients and 90 control sa ... | 1991 | 1662955 |
| effects of sex steroids on immunoglobulin m production by epstein-barr virus-transformed b-cell line skw6-cl4. | the effects of estradiol (e2), progesterone (p), and testosterone (t) on the production of immunoglobulin (ig) m by epstein-barr virus-transformed b-cell line, skw6-cl4, were investigated. interleukin 6 (il-6) induced igm production by skw6-cl4 cells in a dose-dependent manner without any significant increase in thymidine incorporation. e2 at concentrations ranging from 10(-10) to 10(-9) m enhanced il-6-induced igm production by skw6-cl4 cells, whereas e2 at a high concentration of 10(-7) m inhi ... | 1991 | 1663147 |
| immune response to peptides from the third hypervariable region of the beta chain of mhc class ii molecules. implications for the immune response to foreign antigens. | to understand the biologic significance of amino acid sequence sharing between proteins from pathogens and hypervariable regions of hla dr molecules, we studied the immunological status of a peptide from the third hypervariable region of ieb, the mouse equivalent of hla drb. we found that allo mhc peptides are recognized and self mhc peptide is tolerated. this suggests that mhc class ii molecules may modulate the t cell repertoire not only by selective binding of antigenic peptides (determinant ... | 1991 | 1663408 |
| orientation of hla-dna gene and identification of a cpg island-associated gene adjacent to dna in human major histocompatibility complex class ii region. | we have constructed the detailed physical map of the hla class ii gene region by the pulsed field gel electrophoresis (pfge) and cosmid walking technique. in this process, the dna gene was found to be located telomeric to dpa1 with the 5'----3' orientation which is the same as the dpa1 and dpa2 genes, but opposite to the dqa1, dqa2 and dra genes. this orientation is reverse to that of the counterpart gene in the rabbit major histocompatibility complex region. about 30 kb downstream from the dna ... | 1991 | 1663500 |
| fluorescent tetradecanoylphorbol acetate: a novel probe of phorbol ester binding domains. | protein kinase c (pkc) has a prominent role in signal transduction of many bioactive substances. we synthesized the fluorescent derivative, phorbol-13-acetate-12-n-methyl-n-4-(n,n'-di(2-hydroxyethyl)amino)-7-n itr obenz-2-oxa-1,3-diazole-aminododecanoate (n-c12-ac(13)) of 12-o-tetradecanoylphorbol-13-acetate (tpa) to monitor the location of phorbol ester binding sites and evaluate its potential use as a probe of pkc in viable cells. the excitation maximum wavelength of n-c12-ac(13) is close to 4 ... | 1991 | 1663503 |
| amplification and characterization of herpesvirus dna in cerebrospinal fluid from patients with acute encephalitis. | a single pair of oligonucleotide primers selected within a highly conserved region of the dna polymerase gene of the herpesviruses was designed to amplify related viral genomes, i.e., herpes simplex virus type 1, herpes simplex virus type 2, epstein-barr virus, and cytomegalovirus, by the polymerase chain reaction. a simple restriction enzyme analysis of these amplified products allowed accurate characterization of the herpesvirus type. ninety-nine cerebrospinal fluid samples from 36 patients (i ... | 1991 | 1663508 |
| genotypic and phenotypic evidence of t-cell leukemia in a patient successfully treated by interferon-alpha for typical hairy cell leukemia. | a 53-year-old man was diagnosed to have typical hairy cell leukemia. immunophenotyping of frozen splenic tissue showed clonality of hairy cells for mu lambda, confirmed by the corresponding immunoglobulin gene rearrangements. the patient was successfully treated with interferon-alpha (if-alpha). in the fifth year of treatment with if-alpha the morphology of peripheral blood mononuclear cells (pbmc) and of bone marrow infiltration changed with the appearance of numerous small to intermediate shap ... | 1991 | 1663564 |
| sezary cell lymphoma following cyclosporin immunosuppression for renal transplantation. | | 1991 | 1663600 |
| a human anti-jkb monoclonal antibody. | peripheral blood mononuclear cells of an immunized patient were transformed with epstein-barr virus and then fused with p3x63ag8 mouse myeloma cells by polyethylene glycol. after the cloning, a hybridoma cell line secreting specific anti-jkb monoclonal antibody was isolated. the antibody was produced in supernatant form and tested for its use as a blood grouping reagent. | 1991 | 1663674 |
| the b cell repertoire in rheumatoid arthritis. i. frequency of ebv-inducible circulating precursors producing autoantibodies. | the frequency of b cell precursors producing antibodies against various autoantigens (fc fragment of igg, f(ab')2 fragment of igg, type ii collagen, cytoskeleton filaments and insulin) was determined in patients with rheumatoid arthritis (ra) using immortalization of peripheral blood b cells by the epstein-barr virus (ebv) and limiting dilution analysis. equally large numbers of b cell precursors producing igm-rheumatoid factors (rfs) were present in the peripheral blood of seronegative and sero ... | 1991 | 1663752 |
| [the role of persistent epstein-barr herpesvirus infection in the etiology of arteriosclerosis and its complications]. | | 1991 | 1664007 |
| maple syrup urine disease: clinical and biochemical significance of gene analysis. | | 1991 | 1664011 |
| metallothioneins of monocytes and lymphocytes. | | 1991 | 1664023 |
| adenosine deaminase activity in relation to the appearance of early and late epstein-barr virus antigens induced in lymphoblastoid cells. | induction of epstein-barr virus (ebv) capsid antigen synthesis in 59.6% of p3hr-1 cells was followed by a decrease to 70% in adenosine deaminase (ada) activity. in daudi cells synthesizing ebv early antigen, ada activity did not decrease. | 1991 | 1664042 |
| establishment and characterization of a bi-phenotypic leukemic cell line, nalm-19, from a patient with acute leukemia. | based on the immunophenotypic and genotypic findings, this acute leukemia cell line, designated nalm-19, is unique in that a partial expression of both b-cell and myeloid cell features are present in this single clonal leukemic cell population. it is noteworthy that two "normal" eb virus-transformed b cell lines, b239 and b240, (paired with nalm-19) were established from the same leukemic blood. | 1991 | 1664237 |
| distribution of type a and type b ebv in patients with nasopharyngeal carcinoma. | the subtypes of epstein-barr virus (ebv) according to ebna 2 genes were investigated in 53 nasopharyngeal carcinoma (npc) biopsies in taiwan by polymerase chain reaction (pcr) and by southern blot hybridization method. ebv dna was found present in all npc biopsies. type a was the predominant type and comprised of 94.3% of the ebv of npc. type b or coinfection of type a and type b comprised of only 3.8% and 1.9% respectively. | 1991 | 1664280 |
| seroepidemiological correlations of antibodies to human herpesviruses and human immunodeficiency virus type 1 in african patients. | a seroepidemiological evaluation of the humoral immune response against human herpes viruses was carried out in patients with and without hiv infection in tanzania to study the role of these viruses as a cofactor in aids. serum specimens were obtained from 321 outpatients and 100 healthy schoolchildren of a rural population in the kagera region, tanzania, and from 149 inpatients of an urban population in dar-es-salaam, tanzania. the data were analysed by logistic models taking into account demog ... | 1991 | 1664345 |
| expression of epstein-barr virus replicative proteins in aids-related non-hodgkin's lymphoma cells. | epstein-barr virus (ebv) infection in lymphoproliferative lesions has been assumed to be strictly latent. in order to investigate the possible occurrence of ebv replication in aids-related lymphoma (arl) cells, we studied 13 cases by immunohistology using monoclonal antibodies to the ebv-encoded switch-protein bzlf1, early antigens (eas), late replicative proteins [virus capsid antigens (vcas) and membrane antigens (mas)], and to the latent proteins eb nuclear antigen 2 (ebna 2) and latent membr ... | 1991 | 1664459 |
| immune thrombocytopenic purpura evolving into aplastic anemia in association with epstein-barr virus infection. | two children with typical findings of acute immune thrombocytopenic purpura (itp) soon progressed to pancytopenia with severely hypocellular bone marrows. both were found to have evidence of recent epstein-barr virus (ebv) infection. treatment with anti-thymocyte globulin resulted in a complete remission in one patient and near complete remission in the other. possible pathophysiologic mechanisms of this transformation are discussed. children with a diagnosis of itp who suffer a progressive fall ... | 1991 | 1664661 |
| infection-associated hemophagocytic syndrome. evidence for epstein-barr virus gene expression. | autopsy specimens from a patient with infection-associated hemophagocytic syndrome (iahs) were evaluated for the presence of epstein-barr virus dna and rna using in situ hybridization. frozen sections of liver, lymph node, and spleen were probed with ebv bam hi-h & w, gamma interferon, and sp-65 plasmid dna as a negative control probe. hybridization patterns before and after treatment with ribonuclease a were examined. both ebv probes produced diffuse hybridization throughout the tissues; in add ... | 1991 | 1664662 |
| [clinical and pathogenetic observations on children with chronic mononucleosis]. | epstein-barr virus is seldom the causative agent of a prolonged atypical illness, known as chronic mononucleosis syndrome, characterized by a persistent pattern of clinical manifestations and by a defective immune response to specific viral antigens. this paper refers about 6 children for whom clinical and serological findings suggest the chronic epstein-barr virus infection. the authors believe that this chronic state might be explained by the unusual antibody pattern to ebv virus, with the per ... | 1991 | 1664943 |
| epstein-barr virus tissue tropism: a major determinant of immunopathogenesis. | | 1991 | 1664981 |
| molecular basis for epstein-barr virus induced pathogenesis and disease. | | 1991 | 1664982 |
| evolving principles in immunopathology: interleukin 10 and its relationship to epstein-barr virus protein bcrf1. | | 1991 | 1664983 |
| epstein-barr virus infection at mucosal surfaces: detection of genomic variants with altered pathogenic potential. | | 1991 | 1664984 |
| x-linked lymphoproliferative disease (xlp) as a model of epstein-barr virus-induced immunopathology. | | 1991 | 1664985 |
| lymphoproliferative disease in organ transplant recipients. | | 1991 | 1664986 |
| reactivation of epstein-barr virus in sjögren's syndrome. | sjögren's syndrome (ss) is a chronic autoimmune disease characterized by severe dryness of the eyes and mouth, resulting from lymphocytic infiltration of the lacrimal and salivary glands. ss may exist as a primary condition (primary ss, 1.ss) or as a secondary condition (2.ss) in association with rheumatoid arthritis, systemic lupus erythematosus, or progressive systemic sclerosis. in some 1.ss patients, there may be involvement of the extraglandular organs, including skin, kidney, liver, lung a ... | 1991 | 1664987 |
| relationship between antibody production to epstein-barr virus (ebv) early antigens and various ebv-related diseases. | | 1991 | 1664988 |
| control of viral disease: the development of epstein-barr virus vaccines. | | 1991 | 1664989 |
| [pseudo-hairy leukoplakia after renal transplantation in a hiv negative woman]. | | 1991 | 1665048 |
| [malignant otorhinolaryngological lymphomas associated with human immunodeficiency virus infection]. | six instances of lymphoma occurring in homosexual male patients among 140 hiv-positive subjects attended at our department of otolaryngology were evaluated for clinical features, histopathologic features and epstein-barr virus (ebv) dna. the histology of the patients was consistent with a hodgkin's lymphoma, centroblastic lymphoma and four lymphoblastic lymphoma. high malignancy and nodal localization were characteristic of four non-hodgkin's lymphoma, which carries a poor prognosis. the dna in ... | 1991 | 1665072 |
| [new perspectives in the search for a vaccine against the epstein-barr virus]. | | 1991 | 1665087 |
| [prevalence of the epstein-barr virus among different populations in the central area]. | in order to estimate the seroprevalence of the epstein-barr virus in the centre area, a serological study is carried out using the indirect immunofluorescence technique on 759 supposed healthy people, from both sexes and with ages between 6 months and 50 years, 565 from which were living in urban areas (madrid and guadalajara city) and 194 in rural areas (arganda del rey, madrid) and chiloeches (guadalajara). global seroprevalence was of 81% in madrid city, 90% in guadalajara city and 85% in bot ... | 1991 | 1665088 |
| regulation of immune activation/retroviral replication by cd8+ t cells. | | 1991 | 1665323 |
| non-hodgkin's lymphoma after prolonged remission of hodgkin's disease in an hiv-infected patient. | hodgkin's disease was diagnosed in a 22-year-old hiv-seropositive man in 1986. alternate mopp/abvd chemotherapy induced a clinical remission. he was asymptomatic until 3 years later when fever and peripheral and mediastinal lymphadenopathy appeared. lymph node biopsy showed a large-cell anaplastic lymphoma and ebv genome was identified in the malignant cells, suggesting that transformation might had been induced by ebv. the present case affirms that in patients with hiv-related lymphomas who pre ... | 1991 | 1665342 |
| [detection of epstein-barr virus dna in hairy leukoplakia by in situ hybridization]. | hairy leukoplakia (hl) is one of the commonest oral lesions associated with infection with the human immunodeficiency virus (hiv). this entity is regarded to be related to replicative epstein-barr virus (ebv) infection, in the epithelial cells of the oral mucosa. the present study was aimed at demonstrating the presence of ebv in 19 biopsy specimens, with clinical and histologic diagnosis of hl. | 1991 | 1665531 |
| [hairy leukoplakia]. | | 1991 | 1665533 |
| alice in wonderland syndrome as a presenting symptom of ebv infection. | four chinese patients (2 boys, 2 girls), ages 4-9 years, who had episodes of perception error of body schema and objects are reported. the metamorphopsia, which had been named alice in wonderland syndrome, is the major presentation. all of them were proved to have acute epstein-barr virus infection, as documented by positive heterophil antibody test and/or positive igm antibody to epstein-barr virus capsid antigen. the duration of the perception disorder ranged from 1 week to 3 months. we believ ... | 1991 | 1665694 |
| peptide competition at the level of mhc-binding sites using t cell clones from a rheumatoid arthritis patient. | the inhibition of antigen presentation in rheumatoid arthritis by blocking peptide binding to mhc at the antigen presenting cell (apc) level was investigated using various synthetic peptides derived from the 65 kda mycobacterial protein. human t cell clones from tuberculosis and rheumatoid arthritis patients were stimulated with peptides in the presence of irradiated apcs (autologous or dr homozygous ebv-b cell lines). two peptides (residues 65-85 and 412-426) were found to be able to bind to th ... | 1991 | 1665695 |
| transient lupus anticoagulant induced by epstein-barr virus infection. | a 25-year-old woman presented with an episode of left calf deep vein thrombosis and pulmonary thrombosis. she was found to have a lupus anticoagulant with anticardiolipin antibodies, some autoimmune antibodies and antibodies for primary epstein-barr (eb) virus infection. six months later, lupus anticoagulant and other autoimmune antibodies were found to be negative and eb virus antibodies were shown to be seroconverted. we suggest that the transient presence of lupus anticoagulant was due to eb ... | 1991 | 1665993 |
| enhanced frequency of spontaneous b cell lines from epstein-barr virus (ebv) seropositive donors 80 years and older. | based on the well-recognized decline in immunocompetence which develops with advancing age, we have evaluated the effect of age on the frequency of development of spontaneous epstein-barr virus (ebv)-infected b cell lines. blood mononuclear cells were isolated from 38 clinically healthy seropositive donors. the cells were maintained in vitro according to routine culture conditions for lymphocytes. eight spontaneously ebv-infected b lymphoblastoid cell lines (lcl) were isolated. the lcl developed ... | 1991 | 1666055 |
| sinus histiocytosis with massive lymphadenopathy (rosai-dorfman disease) in a patient with elevated epstein-barr virus titers. | sinus histiocytosis with massive lymphadenopathy (shml) is a newly recognized disorder. the etiology of this disease is unknown. an exaggerated response to an offending agent such as the epstein-barr virus or klebsiella bacteria has been postulated. its course is usually benign. cervical adenopathy is seen in 97% of the patients, while 30% of patients have nodal involvement in other sites, and 30% have extranodal involvement. there is a 7% mortality rate that occurs primarily in patients with im ... | 1991 | 1666127 |
| kawasaki disease and epstein-barr virus. | we report the results of virological (serological and molecular biological) studies of epstein-barr virus (ebv) in patients with kawasaki disease (kd). forty-nine (86%) of 57 kawasaki disease patients and 15 (68%) of 22 patients with recurrent kawasaki disease had serological evidence of primary epstein-barr virus infection during the first month after the onset of their disease based on the results of a sensitive method of detecting antibody to viral capsid antigen (vca). the serological respon ... | 1991 | 1666245 |
| ubiquitin and the lysosome system: molecular immunopathology reveals the connection. | considerable advances in our understanding of the molecular pathology of the major human neurodegenerative diseases have been made by the use of ubiquitin immunocytochemistry. the technique demonstrates that filamentous inclusions and vacuoles contain ubiquitin-protein conjugates. the molecular structure of the filaments and the morphological type of vacuoles is not completely understood but there is evidence that some of the filamentous inclusions contain intermediate filaments and the perinucl ... | 1991 | 1666279 |
| oral manifestations of recently described viral infections, including aids. | oral viral diseases secondary to human immunodeficiency virus-1 infection are not commonly observed, except for epstein-barr virus infection. for the most part, members of the herpes virus group and the human papilloma viruses are involved. with the introduction of techniques like the polymerase chain reaction, the level of detection has been changed considerably. however, the application of these techniques may also result in difficulties of interpretation, such as is the case with the detectio ... | 1991 | 1666307 |
| oral manifestations of viral infections in immunocompromised patients. | viral infections are a significant cause of morbidity and mortality in immunosuppressed patients. it is clear that diseases or medical treatments that have cytostatic or cytotoxic effects on lymphocytes and disrupt cytokine production or activity increase the risk of viral infections. while the rate of viral infection varies with the nature and degree of immunosuppression, it is clear that reactivation of latent virus is the most important determinant of the types of viral infections most freque ... | 1991 | 1666308 |
| relationship of viral infection to malignancies. | the epidemiologic features and the biologic mechanisms of the oral tumor viruses have been clarified during the period under review. the use of more sensitive detection methods has confirmed that the prevalence of papillomaviruses in the mouth in patients with various lesions, as well as in healthy individuals, is high. mechanisms by which papillomaviruses are regulated by cells and by which they may control cell behavior have been described. herpes simplex virus has been shown to induce a varie ... | 1991 | 1666309 |
| activation and fixation of c3 by human b cell lines is enhanced by interferon-gamma and tumor necrosis factor alpha. | human b cell lines were tested for the capacity to convert c3 to c3b/ic3b through the alternative c pathway (acp) and to fix the generated c3 fragments. the panel of lines included four epstein-barr virus (ebv)-negative burkitt's lymphomas and their sublines converted with two ebv strains: the prototype b958 (b virus) and the nontransforming p3hr-1 (p virus). comparison of these groups of parallel lines (derived from a single patient) showed that the ebv-carrying cells were more efficient in act ... | 1991 | 1666351 |
| hepatitis-e-virus-associated antigen: improved detection in stools by protein fv removal. | elisa detection of a hepatitis-e-virus-associated antigen (hev-aag) in stools was reappraised for its possible interference with a new fab-binding factor, termed protein fv, released during infectious hepatitis. transaminase elevation, hev-aag discharge and fv leakage appeared simultaneously in a cercopithecus monkey inoculated with infected stools. labelled normal, or immune human igg, were compared with pre- and post-inoculation simian igg, for hev-aag and fv detection. coated normal and patie ... | 1991 | 1666435 |
| virological and clinical characteristics of human herpesvirus 6. | six distinct human herpesviruses have been identified. they include herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), cytomegalovirus (cmv), varicella-zoster virus (vzv), epstein-barr virus and the recently described human herpesvirus 6 (hhv-6). with the exception of hsv-2, the members of the family are ubiquitous and infect most of the human population in the first decade of life. hhv-6 possesses morphological and structural features characteristic of members from the herpesvirus family ... | 1991 | 1666441 |
| vaccination against herpesvirus, fiction or reality? | against herpes simplex type 1 (hsv-1) there are commercially available vaccines which may induce protection against symptoms of primary infections, but not against establishment of latency. effective animal vaccines have been developed against herpes simplex-like viruses, for example pseudorabies vaccine for pigs, and an experimental vaccine can protect cattle against infection with infectious bovine rhinotracheitis (ibr) virus. there are also commercially available effective live vaccines again ... | 1991 | 1666442 |
| herpesvirus infection: an overview of the clinical manifestations. | herpesviruses include seven human viruses and numerous animal viruses. the human herpesviruses, in addition to their trivial names, are named from 1 to 7. the two most recently discovered herpesviruses thus being human herpesvirus 6 and 7. human herpesvirus 6 is the aetiologic agent causing exanthema subitum. human herpesvirus 7 has, as yet, not been associated with any disease. a characteristic feature of the herpesviruses is the persistence in a latent form after primary infection which may la ... | 1991 | 1666443 |
| clinical aspects on epstein-barr virus infection. | epstein-barr virus infection (ebv) was discovered 25 years ago in tumour cells from burkitt's lymphoma. extensive virological studies have relieved that ebv causes infectious mononucleosis and contributes to the pathogenesis of burkitt's lymphoma and nasopharyngeal cancer. atypical courses of the primary infection may induce meningoencephalitis or hepatitis and are attracting increasing attention. antiviral treatment with acyclovir has been administered for 7 days, intravenously or orally, in th ... | 1991 | 1666450 |