| sensitivity of common carp, cyprinus carpio l., strains and crossbreeds reared in the czech republic to infection by cyprinid herpesvirus 3 (cyhv-3; khv). | | 2013 | 23009156 |
| koi herpesvirus encodes and expresses a functional interleukin-10. | koi herpesvirus (khv) (species cyprinid herpesvirus 3) orf134 was shown to transcribe a spliced transcript encoding a 179-amino-acid (aa) interleukin-10 (il-10) homolog (khvil-10) in koi fin (kf-1) cells. pairwise sequence alignment indicated that the expressed product shares 25% identity with carp il-10, 22 to 24% identity with mammalian (including primate) il-10s, and 19.1% identity with european eel herpesvirus il-10 (ahvil-10). in phylogenetic analyses, khvil-10 fell in a divergent position ... | 2012 | 22896613 |
| a model to approximate lake temperature from gridded daily air temperature records and its application in risk assessment for the establishment of fish diseases in the uk. | ambient water temperature is a key factor controlling the distribution and impact of disease in fish populations, and optimum temperature ranges have been characterised for the establishment of a number important aquatic diseases exotic to the uk. this study presents a simple regression method to approximate daily average surface water temperature in lakes of 0.5-15 ha in size across the uk using 5 km(2) gridded daily average air temperatures provided by the uk meteorological office. a geographi ... | 2013 | 22862828 |
| down-regulation of the cyprinid herpesvirus-3 annotated genes in cultured cells maintained at restrictive high temperature. | cyprinid herpesvirus-3 (cyhv-3) is a member of the alloherpesviridae, in the order herpesvirales. it causes a fatal disease in carp and koi fish. the disease is seasonal and is active when water temperatures ranges from 18 to 28 °c. little is known about how and where the virus is preserved between the permissive seasons. the hallmark of the herpesviruses is their ability to become latent, persisting in the host in an apparently inactive state for varying periods of time. hence, it could be expe ... | 2012 | 22841492 |
| spatio-temporal analysis of cyprinid herpesvirus 3 genetic diversity at a local scale. | cyprinid herpesvirus 3 (cyhv-3), the causative agent of koi herpesvirus disease, is a major threat for carp populations in many countries worldwide, including indonesia. it has been shown that many genotypes circulate worldwide, all highly related to one of the two known lineages u/i and j. in this study, we evaluated the spatial and temporal distribution of cyhv-3 strains in a small enzootic area, the lake of cirata (west java, indonesia). of the 365 samples analysed, from clinical or asymptoma ... | 2012 | 22805046 |
| interferon type i responses to virus infections in carp cells: in vitro studies on cyprinid herpesvirus 3 and rhabdovirus carpio infections. | interferons (ifns) are secreted mediators that play a fundamental role in the innate immune response against viruses among all vertebrate classes. common carp is a host for two highly contagious viruses: spring viraemia of carp virus (rhabdovirus carpio, svcv) and the cyprinid herpesvirus 3 (cyhv-3), which belong to rhabdoviridae and alloherpesviridae families, respectively. both viruses are responsible for significant losses in carp aquaculture. in this paper we studied the mrna expression prof ... | 2012 | 22683518 |
| feeding cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry through infection of pharyngeal periodontal mucosa. | cyprinid herpesvirus 3 (cyhv-3), also known as koi herpesvirus, is the etiological agent of a mortal disease in common and koi carp. recently, we investigated the entry of cyhv-3 in carp using bioluminescence imaging and a cyhv-3 recombinant strain expressing luciferase (luc). we demonstrated that the skin is the major portal of entry after inoculation of carp by immersion in water containing cyhv-3. while this model of infection mimics some natural conditions in which infection takes place, oth ... | 2012 | 22276598 |
| identification of dactylogyrus spp. and other parasites of common carp in northeast of iran. | this research was carried out in order to get overview of current status of infestation with dactylogyrus spp. and other parasites in common carp (cyprinus carpio) collected from the ponds around mashhad in northeast of iran. a total of 100 common carps from march to december 2011, 61 (61 %) specimens were hosts to parasites. monogenea dominated the parasite load most of all fishes, whereas argulus foliaceus (arthropoda) was the less parasite. parasites were found as followed: dactylogyrus exten ... | 2012 | 24082535 |
| metazoan parasites of the common carp (cyprinus carpio l., 1758) from tahtalı dam lake (izmir). | the aim of this study was identification of the parasites of the common carp from tahtalı dam lake. | 2012 | 23169163 |
| the first report of dactylogyrus formosus kulwiec, 1927 (monogenea: dactylogyridae) from goldfish (carassius auratus) in central china. | dactylogyrus formosus kulwiec, 1927 (monogenea: dactylogyridae), widely distributed across eurasia, is considered as one of the most frequently encountered ectoparasites of goldfish (carassius auratus). in the present study, d. formosus on gills of cultured goldfish was reported in central china for the first time, using the methods of morphological characters and molecular markers. it was characterized by "anchoratoid-wegeneri" type anchor with elongate inner and unobvious outer root. the morph ... | 2015 | 25899326 |
| intensity of parasitic infestation in silver carp, hypophthalmichthys molitrix. | silver carp, hypopthalmichthys molitrix is one of the most economically valuable fish species in bangladesh. however, its production is often hindered by parasite-induced mortality. the present study reports the intensity of parasitic infestation in 216 specimens of h. molitrix collected from different fish markets in rajshahi city, bangladesh. nine different parasite species (trichodina pediculatus, dactylogyrus vastator, ichthyophthirius multifilis, gyrodactylus elegans, lernaea sp., apiosoma ... | 2012 | 23225858 |
| [composition of leucocytes of the head kidney of the crucian carp carassius auratus gibelio (cypriniformes: cyprinidae) as affected by invasion of cestode digramma interrupta (cestoda; pseudophyllidea)]. | the composition of leucocytes of the head kidney is studied in the crucian carps (carassius auratus) either contaminated or uncontaminated with digramma interrupta. the composition of leucocytes in the pronephros of the crucian carp from lake baikal basin has a lymphoid character. compared to the crucian carp from the european part of russia, in the fish from baikal the granulocytopoetic processes are more pronounced. this is proved by the high content of young forms of granulocytes. in the fish ... | 2016 | 22292296 |
| epidemiology of fishborne trematodiasis in kazakhstan. | fish borne trematodes are an important cause of morbidity in kazakhstan. the number of human cases of opisthorchiidosis (infection with parasites of the family opisthorchiidae) reached a peak of 2521 recorded cases (17 cases per 100,000) in 2002 with a gradual decline to 1225 cases (7.4 cases per 100,000) in 2011. most human cases are found in the north and north east part of kazakhstan in areas drained by the irtysh river and it tributaries. a further focus is found in the north west in the ura ... | 2014 | 24943190 |
| phenotypic traits, virulence-associated gene profile and genetic relatedness of edwardsiella tarda isolates from japanese eel anguilla japonica in korea. | edwardsiella tarda is the predominant bacterium in farm-cultured eel in korea. here, we evaluated the heterogeneity of 37 e. tarda isolates derived from japanese eel with various origins (olive flounder, common carp and ornamental fish) between 2003 and 2010. regardless of origins, the biochemical characteristics of e. tarda isolates were homogenous except hydrogen sulfide production, citrate utilization and mannitol fermentation. based on the phylogenetic analysis of 16s rrna, e. tarda isolates ... | 2014 | 24117973 |
| edwardsiella tarda ompa encapsulated in chitosan nanoparticles shows superior protection over inactivated whole cell vaccine in orally vaccinated fringed-lipped peninsula carp (labeo fimbriatus). | the use of oral vaccination in finfish has lagged behind injectable vaccines for a long time as oral vaccines fall short of injection vaccines in conferring protective immunity. biodegradable polymeric nanoparticles (nps) have shown potential to serve as antigen delivery systems for oral vaccines. in this study the recombinant outer membrane protein a (rompa) of edwardsiella tarda was encapsulated in chitosan nps (np-rompa) and used for oral vaccination of labeo fimbriatus. the rompa purity was ... | 2016 | 27827990 |
| comparative analysis of adaptive immune response after vaccine trials using live attenuated and formalin-killed cells of edwardsiella tarda in ginbuna crucian carp (carassius auratus langsdorfii). | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. although vaccine trials with formalin-killed cells (fkc) have been reported, the vaccinations failed in protect against e. tarda infection. on the other hand, a live attenuated vaccine strategy is effective against edwardsiellosis; however, the mechanism underlying its effectiveness in fish is unclear. in the present study, we compared the adaptive immune responses in fish vaccinated with fkcs and live attenuate ... | 2015 | 25959574 |
| higher antiviral response of rig-i through enhancing rig-i/mavs-mediated signaling by its long insertion variant in zebrafish. | as an intracellular pattern recognition receptor (prr), the retinoic acid-inducible gene-i (rig-i) is responsible for the recognition of cytosolic viral nucleic acids and the production of type i interferons (ifns). in the present study, an insertion variant of rig-i with 38 amino acids inserted in the n-terminal card2 domain, as well as the typical type, named as rig-ia and rig-ib respectively were identified in zebrafish. rig-ia and rig-ib were all up-regulated following the infection of a neg ... | 2015 | 25524497 |
| [a comparison of methods for identifying mast cells of cyprinus carpio]. | to compare different identifying methods of the mast cells of red crucian carp (cyprinus carpio). | 2014 | 25108443 |
| identification of a novel fish granzyme involved in cell-mediated immunity. | granzymes (gzms) are serine proteases released from cytoplasmic granules within cytotoxic t lymphocytes and natural killer (nk) cells. gzms induce apoptosis within virus-infected and transformed cells. in fish as well as mammals, gzms appear to play a major role in inducing target cell death. however, information on the function of fish gzms is limited, although gzm-like genes have been reported in several species. we identified and characterized a fish gzm (termed gcgzm) in ginbuna crucian carp ... | 2014 | 24968079 |
| lack of a contact requirement for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp. | cytotoxic t lymphocytes (ctl) recognize and kill cells infected with viruses, intracellular bacteria and tumors with mhc restriction and antigen specificity. in addition to these activities, recent studies in mammals have suggested that ctl can exhibit direct microbicidal activity. in our previous study we documented direct antibacterial activity of cd4(+) t cells and sigm(+) cells as well as cd8α(+) t cells from immunized fish. however, we also found weak non-specific killing activity of lympho ... | 2014 | 24859594 |
| gc/ms-based metabolomics approach to identify biomarkers differentiating survivals from death in crucian carps infected by edwardsiella tarda. | microbial disease problems constitute the largest single cause of economic losses in aquaculture. an understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. in the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by edwardsiella tarda eib202. we establish the metabolic ... | 2014 | 24837326 |
| role of cd4(+) and cd8α(+) t cells in protective immunity against edwardsiella tarda infection of ginbuna crucian carp, carassius auratus langsdorfii. | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. our previous study suggests that cell-mediated immunity (cmi) plays an essential role in protection against e. tarda infection. in the present study, we adoptively transferred t-cell subsets sensitized with e. tarda to isogenic naïve ginbuna crucian carp to determination the t-cell subsets involved in protecting fish from e. tarda infection. recipients of cd4(+) and cd8α(+) cells acquired significant resistance ... | 2014 | 24316500 |
| adaptive immune response to edwardsiella tarda infection in ginbuna crucian carp, carassius auratus langsdorfii. | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. although cell-mediated immunity and innate immunity play a major role in protection against intracellular bacterial infection in mammals, their importance in protecting fish against e. tarda infection remain unclear. in this study, we examined cell-mediated and humoral immune responses in ginbuna crucian carp (carassius auratus langsdorfii) after e. tarda infection. innate immunity was observed to be the princip ... | 2013 | 23489657 |
| direct antibacterial activity of cd8+/cd4+ t-cells in ginbuna crucian carp, carassius auratus langsdorfii. | cytotoxic t cells (ctls) constitute an important component of the specific effector mechanism in killing against microbial-infected or transformed cells. in addition to these activities, recent studies in mammals have suggested that ctls can exhibit direct antimicrobial activity. therefore, the present investigation was conducted to find out the microbicidal activity of cd8α(+) t cells of ginbuna crucian carp, carassius auratus langsdorfii. the cd8α(+) t cells from immunised ginbuna exhibited th ... | 2013 | 23089524 |
| stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish. | intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ... | 2017 | 28389237 |
| quantitative analyses of the bacterial microbiota of rearing environment, tilapia and common carp cultured in earthen ponds and inhibitory activity of its lactic acid bacteria on fish spoilage and pathogenic bacteria. | the present study aimed to evaluate the bacterial load of water, nile tilapia and common carp intestines from earthen ponds, isolate lactic acid bacteria (lab) and assess their antimicrobial activity against fish spoilage and pathogenic bacteria. following enumeration and isolation of microorganisms the antimicrobial activity of the lab isolates was evaluated. taxonomic identification of selected antagonistic lab strains was assessed, followed by partial characterisation of their antimicrobial m ... | 2017 | 28063102 |
| in vitro and in vivo genotoxicity assessment of selected pharmaceuticals in relation to escherichia coli and cyprinus carpio. | genotoxicity studies (using sos chromotest and comet assay) of escherichia coli and carp (cyprinus carpio) were performed for three pharmaceutically active compounds, ciprofloxacin, 17α-ethinylestradiol and 5-fluorouracil, used in the treatment of humans. the values of genotoxicity induction coefficient (i) in the sos chromotest clearly showed genotoxicity for ciprofloxacin, both in the presence and in the absence of s9 fraction; 17α-ethinylestradiol demonstrated slight genotoxicity at the highe ... | 2016 | 27410723 |
| differential responses to environmental challenge by common carp cyprinus carpio highlight the importance of coping style in integrative physiology. | common carp cyprinus carpio displaying proactive or reactive stress coping styles were acclimated to two environmental regimes (low oxygen and low temperature), and selected groups were tested for response to an inflammatory challenge (escherichia coli lipopolysaccharide, lps). plasma glucose and lactate levels were measured, as were selected c. carpio-specific messenger (m)rna transcript abundance, including cortisol receptor (cr), enolase (eno), glyceraldehyde 3-phosphate dehydrogenase (gapdh) ... | 2016 | 26762295 |
| a cross-reactive human single-chain antibody for detection of major fish allergens, parvalbumins, and identification of a major ige-binding epitope. | fish allergy is associated with moderate to severe ige-mediated reactions to the calcium binding parvalbumins present in fish muscle. allergy to multiple fish species is caused by parvalbumin-specific cross-reactive ige recognizing conserved epitopes. in this study, we aimed to produce cross-reactive single chain variable fragment (scfv) antibodies for the detection of parvalbumins in fish extracts and the identification of ige epitopes. parvalbumin-specific phage clones were isolated from the h ... | 2015 | 26579717 |
| monoclonal antibody against m protein of spring viremia of carp virus. | m protein of spring viremia of carp virus (svcv) was expressed in escherichia coli and then used to immunize balb/c mice. one monoclonal antibody (5a1) against m protein was generated by fusion of mouse myeloma cell line sp2/0 and spleen lymphocytes from immunized mice. the characterizations of this mab were confirmed by elisa (enzyme linked immunosorbent assay), ifa (immunofluorescent assay), and western blot analysis. all results indicate that mab 5a1 was specific to svcv m protein. | 2015 | 25897611 |
| development of a hypoallergenic recombinant parvalbumin for first-in-man subcutaneous immunotherapy of fish allergy. | the fast (food allergy-specific immunotherapy) project aims at developing safe and effective subcutaneous immunotherapy for fish allergy, using recombinant hypoallergenic carp parvalbumin, cyp c 1. | 2015 | 25765512 |
| effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile jian carp (cyprinus carpio var. jian). | this study was conducted to investigate the effects of dietary isoleucine (ile) on the immune response, antioxidant status, tight junctions, and microbial population in the intestine of juvenile jian carp (cyprinus carpio var. jian). a total of 1200 juvenile jian carp with average initial weight 6.9 ± 0.03 g were fed semi-purified isonitrogenous diets containing 4.2 (unsupplemented control group), 7.0, 9.5, 11.9, 13.9 and 16.9 g ile kg(-1) diet for 60 days. results indicated that ile supplementa ... | 2014 | 25451003 |
| monoclonal antibodies against g protein of spring viremia of carp virus. | part of the g protein (3094-4170 bp) of spring viremia of carp virus (svcv) was expressed in escherichia coli and purified by dialysis in our study. two clones of monoclonal antibodies (mabs 1h11 and 4b8) against g protein were generated by fusion of mouse myeloma cell line sp2/0 and spleen lymphocytes from part of g protein (3094-4170 bp) immunized mice. the results of elisa (enzyme-linked immunosorbent assay), ifa (indirect immunofluorescent assay), and western blot assay further demonstrated ... | 2014 | 25358003 |
| expression and characterization of common carp (cyprinus carpio) matrix metalloproteinase-2 and its activity against type i collagen. | matrix metalloproteinases (mmps) play essential roles in the metabolism of animal collagen while few reports are available for mmps in aquatic animals. in this study, we report the complete sequence of matrix metalloproteinase-2 (mmp-2) gene from common carp (cyprinus carpio) skeletal muscle. the full-length cdna of mmp-2 was 2792bp which contains an open reading frame of 1974bp, corresponding to a protein of 657 amino acid residues. based on the structural feature of mmp-2, the gene of the cata ... | 2014 | 24613299 |
| anti-platelet-activating factor, antibacterial, and antiradical activities of lipids extract from silver carp brain. | epidemiological studies have verified the protective role of fish lipids in cardiovascular diseases. however, the effects of fish lipids on health boost remain undefined. large amounts of by-products, such as fish brain which contains high level of lipids, are produced with silver carp processing. fish brain is rich in bioactive lipids which are overwhelmingly effective in preventing cardiovascular diseases. the aim of this study was to elucidate the pharmacological activities of silver carp bra ... | 2013 | 23805935 |
| cdna cloning of glucose-6-phosphate isomerase from crucian carp (carassius carassius) and expression of the active region as myofibril-bound serine proteinase inhibitor in escherichia coli. | glucose-6-phosphate isomerase (gpi) (ec 5.3.1.9) can act as a myofibril-bound serine proteinase (mbsp) inhibitor (mbspi) in fish. in order to better understand the biological information of the gpi and its functional domain for inhibiting mbsp, the cdna of gpi was cloned from crucian carp (carassius carassius) with rt-pcr, nested-pcr and 3'-race. the result of sequencing showed that the gpi cdna had an open reading frame of 1662bp encoding 553 amino acid residues. after constructing and comparin ... | 2014 | 23774640 |
| new vectors derives from puc 18 for clonig and thermal-induced expression in escherichia coli. | we report the construction of two vectors for escherichia coli: puc72, for molecular cloning, and pplt7, for thermal-induced expression. the main feature of puc72 is a novel polylinker region that includes restriction sites for nde i and nco i which provide an atg codon for proper translation initiation of expressed genes. vector pplt7 is ideal for thermo-inducible expression in host cells that carry the ci857 repressor gene. the use of pplt7 was validated by the successful expression of the gen ... | 2009 | 24031424 |
| amplification, cloning, and sequence comparison of the growth hormone gene for carp (cyprinus carpio) by the polymerase chain reaction. | total rna was isolated from carp pituitary gland. the first strand cdna was synthesized using oligo(dt) 12-18 as a primer, the total rna as a template, and amv reverse transcriptase. next the polymerase chain reaction (pcr) was performed using the first strand cdna as a template, the synthetic 29 oligonucleotides as primer and taq dna polymerase (94 degrees c, 60 s; 55 degrees c, 30 s; 72 degrees c, 50 s; 35 cycles). after pcr amplification, the products were cloned into an e. coli expression ve ... | 1996 | 9093758 |
| preparation and comparison of biological properties of recombinant carp (cyprinus carpio) growth hormone and its cys-123 to ala mutant. | carp growth hormone (cgh) cdna, in which cys-123 was mutated to ala, was prepared, transferred to the expression vector, expressed in escherichia coli and the mutant was purified to homogeneity. the mutation only slightly improved yield of the monomeric fraction, indicating that cys-123 is not involved in improper refolding. as compared to cgh, the mutant (cgh-c123a) exhibited lower binding affinity toward homologous liver receptors and lower bioactivity in a 3t3-f442a preadipocyte bioassay desp ... | 1993 | 24202495 |
| major acid endopeptidases of the blood-feeding monogenean eudiplozoon nipponicum (heteronchoinea: diplozoidae). | in parasitic flatworms, acid endopeptidases are involved in crucial processes, including digestion, invasion, interactions with the host immune system, etc. in haematophagous monogeneans, however, no solid information has been available about the occurrence of these enzymes. here we aimed to identify major cysteine and aspartic endopeptidase activities in eudiplozoon nipponicum, an invasive haematophagous parasite of common carp. employing biochemical, proteomic and molecular tools, we found tha ... | 2016 | 26888494 |
| disseminated infection due to exophiala pisciphila in cardinal tetra, paracheirodon axelrodi. | cardinal tetra, paracheirodon axelrodi (schultz, 1956), kept in an ornamental tank, was found to be affected by severe invasive mycosis. externally, the disease manifested as abdominal swelling, and internally, the anterior part of the intestine was extremely bloated with abundant dematiaceous septate hyphae and an accumulation of fluid. histopathologically, a granulomatous inflammatory response was observed in the intestine wall, kidney and spleen. we assume that the mycotic agent was primarily ... | 2016 | 27982440 |
| profilings of micrornas in the liver of common carp (cyprinus carpio) infected with flavobacterium columnare. | micrornas (mirnas) play important roles in regulation of many biological processes in eukaryotes, including pathogen infection and host interactions. flavobacterium columnare (fc) infection can cause great economic loss of common carp (cyprinus carpio) which is one of the most important cultured fish in the world. however, mirnas in response to fc infection in common carp has not been characterized. to identify specific mirnas involved in common carp infected with fc, we performed microrna seque ... | 2016 | 27092486 |
| interactions of highly and low virulent flavobacterium columnare isolates with gill tissue in carp and rainbow trout. | the interactions of flavobacterium columnare isolates of different virulence with the gills of carp (cyprinus carpio l.) and rainbow trout (oncorhynchus mykiss walbaum) were investigated. both fish species were exposed to different high (hv) or low virulence (lv) isolates and sacrificed at seven predetermined times post-challenge. histopathological and ultrastructural examination of carp and rainbow trout inoculated with the hv-isolate disclosed bacterial invasion and concomitant destruction of ... | 2015 | 25889257 |
| gill infection model for columnaris disease in common carp and rainbow trout. | challenge models generating gill lesions typical for columnaris disease were developed for the fry of both common carp cyprinus carpio and rainbow trout oncorhynchus mykiss by means of an immersion challenge and flavobacterium columnare field isolates were characterized regarding virulence. carp inoculated with highly virulent isolates revealed diffuse, whitish discoloration of the gills affecting all arches, while in trout mostly unilateral focal lesions, which were restricted to the first two ... | 2015 | 25488182 |
| phenotypic characterization and genetic diversity of flavobacterium columnare isolated from red tilapia, oreochromis sp., in thailand. | flavobacterium columnare is the aetiological agent of columnaris disease and severely affects various freshwater aquaculture fish species worldwide. the objectives of this study were to determine the phenotypic characteristics and genetic variability among f. columnare isolates isolated from red tilapia in thailand. forty-four f. columnare isolates were recovered from diseased fish in different geographical locations. the isolates exhibited homologous phenotypic characteristics but exhibited gen ... | 2014 | 25287048 |
| susceptibility of common carp and sunfish to a strain of francisella noatunensis subsp. orientalis in a challenge experiment. | francisellosis, an emerging disease in many fish species, can cause high mortality in affected populations. here we investigated the susceptibility of common carp cyprinus carpio and sunfish lepomis gibbosus to francisella noatunensis subsp. orientalis (fno), and possible transmission of the bacteria between the 2 fish species. in a challenge experiment, 3 groups of each species were injected intraperitoneally (ip) with 3 different doses of an fno strain no. 9449 of the norwegian veterinary inst ... | 2016 | 27667813 |
| susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to haplorchis taichui. | fish-borne zoonotic trematodes (fzt) including heterophyids and opisthorchiids are prevalent in native and aquaculture fish in southeast asia. fzt are reported highly prevalent in juvenile aquaculture fish that belonged to the family heterophyidae, particularly haplorchis taichui. aquaculture fish are reported to have varying levels of natural infection with h. taichui, but data for their susceptibility as well as resistance to infection are not available. the aim of this study was to determine ... | 2015 | 25636245 |
| closing the serological gap: promising novel biomarkers for the early diagnosis of rheumatoid arthritis. | rheumatoid arthritis (ra) is a chronic autoimmune disease characterized by inflammation and damage of the joints affecting about 0.5% of the general population. early treatment in ra is important as it can prevent disease progression and irreversible damage of the joints. despite the high diagnostic value of anti-citrullinated protein antibodies (acpa) and rheumatoid factor (rf), there is a strong demand for novel serological biomarkers to further improve the diagnosis of this abundant disease. ... | 2012 | 22664776 |
| establishment, characterization and viral susceptibility of two cell lines derived from leopard wrasse macropharyngodon geoffroy. | two new fish cell lines were established from skin (lwsk) and fin (lwfn) of leopard wrasse macropharyngodon geoffroy. these cells grew optimally at 25° c in leibovitz-15 medium supplemented with 10% foetal bovine serum. proliferation of m. geoffroy cells remained serum dependent up to cell passage 16, and cell-plating efficiency ranged from 12 to 16%. karyotypic analysis of these new cell lines at cell passage 8 indicated that both cell lines remained diploid with a peak chromosomal count of 144 ... | 2013 | 23991874 |
| detection of infectious pancreatic necrosis virus from the leeches hemiclepsis marginata and hirudo medicinalis. | leeches have been reported to harbor several important fish pathogens, including spring viremia of carp virus, infectious hematopoietic necrosis virus (ihnv), and viral hemorrhagic septicemia virus (vhsv), and also may contain blood protozoa. in the present study, leeches were collected from water bodies located in kurdistan province, iran. the specimens were tested for ihnv, vhsv, and infectious pancreatic necrosis virus (ipnv) using the pcr method. the results showed that two different species ... | 2016 | 27687675 |
| inhibition of serpine1 reduces rhabdoviral infections in zebrafish. | while exploring the molecular mechanisms behind the fin hemorrhages that follow zebrafish (danio rerio) early infection with viral haemorrhagic septicemia virus (vhsv), we discovered that most serpin (serine protease inhibitor) gene transcripts were upregulated, except those of serpine1. surprisingly, only serpine1-derived 14-mer peptide and low molecular weight drugs targeting serpine1 (i.e. tannic acid, egcg, tiplaxtinin) inhibited in vitro infections not only of vhsv, but also of other fish r ... | 2015 | 26363229 |
| susceptibility of koi and yellow perch to infectious hematopoietic necrosis virus by experimental exposure. | infectious hematopoietic necrosis virus (ihnv) is a novirhabdoviral pathogen that originated in western north america among anadromous pacific salmonids. severe disease epidemics in the late 1970s resulting from ihnv's invasion into farmed rainbow trout oncorhynchus mykiss in north america, asia, and europe emphasized ihnv's ability to adapt to new hosts under varying rearing conditions. yellow perch perca flavescens and koi carp cyprinus carpio (hereafter, "koi") are aquaculture-reared fish tha ... | 2014 | 24895860 |
| fish tapeworm khawia sinensis: an indicator of environmental microcystins? | parasites have recently been recognized as accumulation indicators that take up and bio-concentrate substances from environmental pollution. interestingly, helminths of fish are known to accumulate metals from the ambient environment and to contain several orders of magnitude higher concentrations than hosts. while the majority of reports mention inorganic toxin accumulation in parasites, studies concerning effects of organic pollution are infrequent and little is known about the potential of pa ... | 2013 | 24362088 |
| cytocomposition of the vitellarium in khawia sinensis hsü, 1935 (cestoda, caryophyllidea, lytocestidae): another caryophyllidean species with lamellar bodies and lipids. | the vitellarium of the invasive caryophyllidean tapeworm khawia sinensis hsü, 1935 from carp cyprinus carpio l. was examined by means of transmission electron microscopy and cytochemical staining for glycogen with periodic acid-thiosemicarbazide-silver proteinate (pa-tsc-sp). a vitellarium consists of numerous follicles of irregular size that are interconnected by a net of vitelline ducts. vitelline follicles are composed of vitelline cells at various stages of development that are interconnecte ... | 2013 | 23749090 |
| sequence structure and intragenomic variability of ribosomal its2 in monozoic tapeworms of the genus khawia (cestoda: caryophyllidea), parasites of cyprinid fish. | the sequence structure of the ribosomal internal transcribed spacer 2 (its2) was determined for six species of khawia (cestoda: caryophyllidea), parasites of cyprinid fish in the holarctic region. homologous intragenomic its2 structure was found in khawia armeniaca, khawia baltica, and khawia rossittensis; whereas divergent intragenomic its2 copies were detected in chinese, japanese, and slovak isolates of khawia sinensis and in khawia japonensis, both parasitic in common carp, and in khawia sau ... | 2012 | 22814768 |
| generation of oxyradicals (o2. and h2o2), mitochondrial activity and induction of apoptosis of pbmc of cyprinus carpio carpio treated in vivo with halomethanes and with recombinant hsp60 kda and with lps of klebsiella pneumoniae. | halomethanes (hm) can be immunotoxic in mammals; however, in the fish immune system hm effects are unknown. in the current study, we evaluated the mitochondrial activity (ma) by mtt, induction of apoptosis by subg0 technique and quantified serum ros concentration (o2. and h2o2) and ros production in pbmc of cyprinus carpio carpio treated i.p. with ch2cl2, chcl3 and brchcl2 (0.004-40.0 mg/kg) for 96 h. positive controls were recombinant heat shock protein of 60 kda (rhsp60 kda) of klebsiella pneu ... | 2014 | 25093392 |
| 16s rrna pcr-denaturing gradient gel electrophoresis of oral lactobacillus casei group and their phenotypic appearances. | this study aimed to develop a 16s rrna pcr-denaturing gradient gel electrophoresis (dgge) to identify the species level of lactobacillus casei group and to investigate their characteristics of acid production and inhibitory effect. pcr-dgge has been developed based on the 16s rrna gene, and a set of hda-1-gc and hda-2, designed at v2-v3 region, and another set of carp-1-gc and carp-2, designed at v1 region, have been used. the bacterial strains included l. casei atcc 393, l. paracasei ccug 32212 ... | 2013 | 24191230 |
| a comparison of endogenous and microbial proteolytic activities during fast fermentation of silver carp inoculated with lactobacillus plantarum. | the study was aimed to investigate different roles of endogenous and lactobacillus plantarum proteases during fast fermentation of silver carp. the results show that endogenous proteases could degrade both sarcoplasmic and myofibrillar proteins. in contrast, l. plantarum had low proteinase activities and could only hydrolyze sarcoplasmic peptides. this indicates that gel properties could be mainly affected by endogenous proteolysis while microbial proteolysis contributed to the production of sma ... | 2016 | 27080883 |
| biogenic amine accumulation in silver carp sausage inoculated with lactobacillus plantarum plus saccharomyces cerevisiae. | the effect of an amine-negative mixed starter culture (lactobacillus plantarum zy40 plus saccharomyces cerevisiae jm19) on biogenic amine accumulation in fermented silver carp sausage was studied. microbial counts, ph, titratable acid and free amino acids were also determined. putrescine, cadaverine and tyramine were the main amines formed during sausage fermentation. the contents of putrescine and cadaverine were greatly reduced by the addition of l. plantarum zy40 plus s. cerevisiae jm19, wher ... | 2014 | 24491750 |
| isolation and characterization of a ranavirus from koi, cyprinus carpio l., experiencing mass mortalities in india. | we investigated mass mortalities of koi, cyprinus carpio linnaeus, 1758, experienced in south indian fish farms by virus isolation, electron microscopy, pcr detection, sequencing of capsid protein gene and transmission studies. samples of moribund koi brought to the laboratory suffered continuous mortality exhibiting swimming abnormalities, intermittent surfacing and skin darkening. irido-like virus was isolated from the infected fish in the indigenous snakehead kidney cell line (snkd2a). icosah ... | 2015 | 24720625 |
| crystal structure of the c-terminal domain of tubulin-binding cofactor c from leishmania major. | tubulin-binding cofactor c stimulates gtpase activity and contributes to the release of the heterodimeric α/β-tubulin from a super-complex of tubulin monomers and two ancillary cofactors. we have determined the 2.2 å resolution crystal structure of the c-terminal domain of tubulin-binding cofactor c from leishmania major based on single wavelength anomalous dispersion measurements targeting a selenomethionine derivative. although previously predicted to consist of two domains the structure is be ... | 2015 | 25982270 |
| dynamics and effects of ligula intestinalis (l.) infection in the native fish barbus callensis valenciennes, 1842 in algeria. | the dynamics of the emergence, duration, and decline phases in epizootic cycles are well known for humans and some crops, but they are poorly understood for host-parasite systems in the wild. parasites may be particularly insidious as they are often introduced unintentionally, simultaneously with their hosts, and later transferred to species in the new location. here we investigate the epizootic dynamics of the tapeworm ligula intestinalis in the hamiz reservoir, algeria, and explore its effects ... | 2016 | 27078654 |
| [epizootological characterization of trematodiasis foci in the ecosystem of the amur river in the khabarovsk territory]. | investigations were conducted in late june to early june 2013. the population density of mollusks of the genus juga in the shoreline and littoral covered by meadow waters was 10 to 30 specimens/m2; there were solitary specimens of the genus parafossarulus. fourteen out of 39 fish species were invaded by metacercariae of clonorchis. manchurian spiny loaches (leptobotia) and light's bitterling (rhodeus lighti) are first registered to be hosts of clonorchis. the metacercariae of metagonimus yokogaw ... | 2015 | 26152032 |
| mixed mycobacterial infection in an adult koi carp cyprinus carpio l. | | 2014 | 23937602 |
| molecular and functional characterization of the scavenger receptor cd36 in zebrafish and common carp. | cd36 is a scavenger receptor which has been studied closely in mammals where it is expressed by many different cell types and plays a role in highly diverse processes, both homeostatic and pathologic. it is among other things important in the innate immune system, in angiogenesis, and in clearance of apoptotic cells, and it is also involved in lipid metabolism and atherosclerosis. recently, in the cephalochordate amphioxus a primitive cd36 family member was described, which was present before th ... | 2015 | 25306962 |
| development of vaccines against nocardiosis in fishes. | nocardiosis, one of the most systemic and devastating diseases, is currently emerging as an important disease of cultured marine and freshwater fishes. the causative agent of this disease is nocardia seriolae, a gram-positive acid-fast bacterium. an effective vaccine/vaccination strategy against this pathogen is necessary to control the significant loss in aquaculture practices. in this chapter, we present the vaccination/immunization protocol in fish against both live (sublethal) and inactivate ... | 2016 | 27076299 |
| immune responses to live and inactivated nocardia seriolae and protective effect of recombinant interferon gamma (rifn γ) against nocardiosis in ginbuna crucian carp, carassius auratus langsdorfii. | looking into the fact that substantial mortality and morbidity is associated with intracellular gram +ve bacterium, nocardia seriolae infection, an effective vaccine against this pathogen is necessary to control the significant losses in aquaculture practices. therefore, an attempt was made to evaluate the effect of live (sub-lethal) and inactivated (antigenic form) n. seriolae on cellular and humoral immunity in ginbuna crucian carp, carassius auratus langsdorfii as well as the therapeutic pote ... | 2014 | 24882019 |
| sensitization rates for various allergens in children with allergic rhinitis in qingdao, china. | the aim of this study was to determine the prevalence of sensitization to common allergens in children with allergic rhinitis (ar) living in qingdao, china. we conducted a retrospective analysis for ar cases, who underwent skin prick tests (spt) in qingdao. a total of 2841 children with ar qualified for the inclusion criteria (age 3-5 years: 1500 children; age 6-12 years: 1341 children). the most common inhaled allergens to which the ar children were sensitive were dermatophagoides pteronyssinus ... | 2015 | 26371014 |
| female morphology and systematic status of philometroides cyprini (nematoda: philometridae), a parasite of carp. | gravid females of philometroides were found under the scales of stock carp cyprinus carpio l. imported from lithuania to the czech republic in the spring of 1997 and kept in a quarantine pond. these specimens and those obtained from pond-cultured carp in russia (moscow district) were used for detailed morphological studies, including scanning electron microscopy. the results confirm that the valid name of this parasite is philometroides cyprini (ishii, 1931), whereas philometra lusii vismanis, 1 ... | 2005 | 16385815 |
| integrative taxonomy of central european parasitic flatworms of the family prosthogonimidae lühe, 1909 (trematoda: plagiorchiida). | species of the family prosthogonimidae are considered the most pathogenic poultry trematodes worldwide, affecting particularly low intensity farming in rural areas. adults of prosthogonimus occur mainly in the bursa of fabricius, oviduct and cloaca of ducks, geese, fowl and other birds feeding at least occasionally on dragonflies or damselflies (odonata). we analyzed the central european species of the prosthogonimidae, namely prosthogonimus cuneatus, prosthogonimus ovatus, prosthogonimus pelluc ... | 2015 | 25724855 |
| isolation of infectious microalga prototheca wickerhamii from a carp (cyprinus carpio) - a first confirmed case report of protothecosis in a fish. | protothecosis is a rare infection caused by environmentally ubiquitous achlorophyllic microalgae of the genus prototheca. here, we describe a first case of protothecosis in a carp (cyprinus carpio), which is at the same time the first case of protothecosis in a fish, confirmed by phenotype- and molecular-based methods, including pcr sequencing of the rdna cluster and protein profiling using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | 2017 | 28300290 |
| the pseudomonas aeruginosa pao1 two-component regulator carsr regulates calcium homeostasis and calcium-induced virulence factor production through its regulatory targets caro and carp. | pseudomonas aeruginosa is an opportunistic human pathogen that causes severe, life-threatening infections in patients with cystic fibrosis (cf), endocarditis, wounds, or artificial implants. during cf pulmonary infections, p. aeruginosa often encounters environments where the levels of calcium (ca(2+)) are elevated. previously, we showed that p. aeruginosa responds to externally added ca(2+) through enhanced biofilm formation, increased production of several secreted virulence factors, and by de ... | 2016 | 26755627 |
| targeting and stimulation of the zebrafish (danio rerio) innate immune system with lps/dsrna-loaded nanoliposomes. | herein we report the use of immunostimulant-loaded nanoliposomes (called nlcliposomes) as a strategy to protect fish against bacterial and/or viral infections. this work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of their in vivo dynamics and characterisation of their tissue distribution. the nlc liposomes, which co-encapsulate poly(i:c) and lps, accumulate in immune tissues and in immunologically relevant cells such ... | 2014 | 24837767 |
| inhibition of spring viraemia of carp virus replication in an epithelioma papulosum cyprini cell line by rnai. | spring viraemia of carp virus (svcv) is an aetiological agent of a serious disease affecting carp farms in europe and is a member of the rhabdoviridae family of viruses. the genome of svcv codes for five proteins: nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g) and rna-dependent rna polymerase (l). rna-mediated interference (rnai) by small interfering rnas (sirnas) is a powerful tool to inhibit gene transcription and is used to study genes important for viral replicat ... | 2015 | 24460815 |
| first report of rahnella aquatilis infection in crucian carp carassius auratus in china. | rahnella aquatilis infection is rare in aquaculture. here, a gram-negative rod-shaped bacterium was isolated from diseased crucian carp carassius auratus in xuzhou city, jiangsu province, eastern china. the isolate was tentatively named strain kcl-5, and subsequently identified as r. aquatilis by biochemical properties and molecular techniques. the results showed that the isolate kcl-5 was most closely related to the type strain atcc33071 (= dsm4594) of r. aquatilis, which shared 99.67, 96.26 an ... | 2017 | 28322207 |
| establishment of a new cell line from the heart of giant grouper, epinephelus lanceolatus (bloch), and its application in toxicology and virus susceptibility. | a new marine fish cell line, derived from the heart of giant grouper, epinephelus lanceolatus (bloch), was established and characterized. the cell line was designated as elgh and subcultured with more than 60 passages. the elgh cells were mainly composed of fibroblast-like cells and multiplied well in leibovitz's l-15 medium supplemented with 10% foetal bovine serum (fbs) at 28 °c. chromosome analysis indicated that the modal chromosome number was 48. the fluorescent signals were detected in elg ... | 2015 | 24372271 |
| medaka haploid embryonic stem cells are susceptible to singapore grouper iridovirus as well as to other viruses of aquaculture fish species. | viral infection is a challenge in high-density aquaculture, as it leads to various diseases and causes massive or even complete loss. the identification and disruption of host factors that viruses utilize for infection offer a novel approach to generate viral-resistant seed stocks for cost-efficient and sustainable aquaculture. genetic screening in haploid cell cultures represents an ideal tool for host factor identification. we have recently generated haploid embryonic stem (es) cells in the la ... | 2013 | 23828270 |
| biologically active recombinant carp lh as a spawning-inducing agent for carp. | currently, spawning is induced in carp species by carp pituitary extract (cpe) and a combination of synthetic agonist of gnrh combined with a dopamine antagonist. the main goal of this study was the production of recombinant gonadotropins (gths) on a large scale to serve as an alternative to currently used agents. we produced carp (c) recombinant (r) lh as a single chain in the methylotrophic yeast pichia pastoris lha subunit was joined with lhb subunit with a flexible linker of three glycine-se ... | 2017 | 27999090 |
| gonadotropins in the russian sturgeon: their role in steroid secretion and the effect of hormonal treatment on their secretion. | in the reproduction process of male and female fish, pituitary derived gonadotropins (gths) play a key role. to be able to specifically investigate certain functions of luteinizing (lh) and follicle stimulating hormone (fsh) in russian sturgeon (acipenser gueldenstaedtii; st), we produced recombinant variants of the hormones using the yeast pichia pastoris as a protein production system. we accomplished to create in vitro biologically active heterodimeric glycoproteins consisting of two associat ... | 2016 | 27622546 |
| no evidence of exposure to environmental estrogens in two feral fish species sampled from the yarra river, australia: a comparison with northern hemisphere studies. | environmental estrogens originate from a variety of sources including sewage treatment plant (stp) effluents and adverse physiological effects (endocrine disruption) have been observed in several fish species sampled downstream of stp discharges. in this study we examined common carp (cyprinus carpio) and roach (rutilis rutilis) for signs of exposure to environmental estrogens in the iconic yarra river, melbourne, australia. the yarra river flows through the city of melbourne and more than 2 mil ... | 2016 | 27228034 |
| srv2/cap is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis. | the dynamic assembly and disassembly of actin filaments is essential for the formation and transport of vesicles during endocytosis. in yeast, two types of actin structures, namely cortical patches and cytoplasmic cables, play a direct role in endocytosis, but how their interaction is regulated remains unclear. here, we show that srv2/cap, an evolutionarily conserved actin regulator, is required for efficient endocytosis owing to its role in the formation of the actin patches that aid initial ve ... | 2016 | 26604224 |
| recombinant saccharomyces cerevisiae serves as novel carrier for oral dna vaccines in carassius auratus. | oral delivery of dna vaccines represents a promising vaccinating method for fish. recombinant yeast has been proved to be a safe carrier for delivering antigen proteins and dnas to some species in vivo. however, whether recombinant yeast can be used to deliver functional dnas for vaccination to fish is still unknown. in this study, red crucian carp (carassius auratus) was orally administrated with recombinant saccharomyces cerevisiae harboring cmv-egfp expression cassette. on day 5 post the firs ... | 2015 | 26481518 |
| the fate of recent duplicated genes following a fourth-round whole genome duplication in a tetraploid fish, common carp (cyprinus carpio). | whole genome duplication (wgd) results in extensive genetic redundancy. in plants and yeast, wgd is followed by rapid gene deletions and intense expression differentiation with slow functional divergence. however, the early evolution of the gene differentiation processes is poorly understood in vertebrates because almost all studied wgds are extremely ancient, and the genomes have returned to a diploid status. common carp had a very recent fourth round of wgd dated to 8 million years ago. it the ... | 2015 | 25645996 |
| investigation of effect of 17α-ethinylestradiol on vigilin expression using an isolated recombinant antibody. | vigilin, a highly conserved protein from yeast to mammals, is a multifunctional protein in eukaryotic organisms. one biological function of vigilin is to stabilize the expression level of vitellogenin (vtg). this study aimed to develop vigilin as a new estrogen-inducible biomarker that correlates with the widely applied estrogen-inducible biomarker vtg and expand the ability to detect it in various species. here, a recombinant monoclonal antibody with high specificity against the conserved c-ter ... | 2014 | 25112681 |
| molecular characterization and expression analysis of the autophagic gene beclin 1 from the purse red common carp (cyprinus carpio) exposed to cadmium. | beclin 1, the mammalian orthologue of yeast atg6, has a central role in autophagy, which has been linked to diverse biological processes including immunity, development, tumor suppression, lifespan extension, etc. however, the relevant study about beclin 1 is rare in fish compared with mammals. in this study, we isolated beclin 1 gene from the kidney tissue of common carp (cyprinus carpio) using rapid amplification of cdna ends (race). the deduced amino acid sequence of cloned beclin 1 comprised ... | 2014 | 24291087 |
| effects of a dietary β-(1,3)(1,6)-d-glucan supplementation on intestinal microbial communities and intestinal ultrastructure of mirror carp (cyprinus carpio l.). | to assess the effects of dietary saccharomyces cerevisiae β-(1,3)(1,6)-d-glucan supplementation (macrogard(®)) on mirror carp (cyprinus carpio l.) intestinal microbiota and ultrastructure of the enterocyte apical brush border. | 2013 | 23889777 |
| oxidative burst and nitric oxide responses in carp macrophages induced by zymosan, macrogard(®) and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors. | β-glucans are glucose polymers that are found in the cell walls of plants, bacteria, certain fungi, mushrooms and the cell wall of baker's yeast. in mammals, myeloid cells express several receptors capable of recognizing β-glucans, with the c-type lectin receptor dectin-1 in conjunction with toll-like receptor 2 (tlr2), considered key receptors for recognition of β-glucan. in our studies to determine the possible involvement of these receptors on carp macrophages a range of sources of β-glucans ... | 2013 | 23831551 |
| effects of dietary β-(1,3)(1,6)-d-glucan supplementation on growth performance, intestinal morphology and haemato-immunological profile of mirror carp (cyprinus carpio l.). | in recent years, aquaculture research has focused on probiotics, prebiotics, and β-glucans, in order to improve health status and growth performance. information regarding the effects of β-glucan on growth performance and intestinal immunity of mirror carp (cyprinus carpio l.) is scarce. an experiment was therefore conducted to investigate the effects of a yeast β-glucan preparation (macrogard(®) ) on growth performance, intestinal morphology and haemato-immunological indices of mirror carp. car ... | 2014 | 23676107 |
| the effect of dietary organic chromium on specific growth rate, tissue chromium concentrations, enzyme activities and histology in common carp, cyprinus carpio l. | a 63-day feeding trial was carried out to investigate the effect of three levels of cr yeast (0.5, 1.0 and 2.0 mg cr/kg) on the utilization of diets containing 38.5 % of maize starch or dextrin in common carp, cyprinus carpio l. (initial mean body mass 14 ± 0.3 g) in an auto circulator system at 25 ± 0.5 °c. a two-way analysis of variance (anova) showed that the final body mass (fbm), percentage mass gain (%mg), specific growth rate (sgr) and feed conversion ratio (fcr) were significantly (p < 0 ... | 2012 | 22569805 |
| astragalin from cassia alata induces dna adducts in vitro and repairable dna damage in the yeast saccharomyces cerevisiae. | reverse phase-solid phase extraction from cassia alata leaves (carp) was used to obtain a refined extract. higher than wild-type sensitivity to carp was exhibited by 16 haploid saccharomyces cerevisiae mutants with defects in dna repair and membrane transport. carp had a strong dpph free radical scavenging activity with an ic(50) value of 2.27 μg ml(-1) and showed no pro-oxidant activity in yeast. carp compounds were separated by hplc and the three major components were shown to bind to dna in v ... | 2012 | 22489129 |
| the efficacy of chromium as a growth enhancer for mirror carp (cyprinus carpio l): an integrated study using biochemical, genetic, and histological responses. | a growth trial was conducted on juvenile mirror carp (cyprinus carpio l.) for 8 weeks to compare the efficacy of three chromium (cr) compounds (cr chloride, cr picolinate, and cr yeast) at a level 0.5 mg/kg as a potential growth enhancer. in addition, a high level of cr (2.0 mg/kg) as cr chloride has also been added in parallel for comparison. all cr fortified diets at a level 0.5 mg/kg produced superior growth for carp compared to the control group and the group fed the high level of cr chlorid ... | 2012 | 22351105 |
| hypolipidaemic foods in china. | with the changes in dietary pattern in china, in recent years, hyperlipidaemia has become an important problem in the pathogenesis of chronic degenerative diseases, especially the cardio-cerebro-vascular diseases. from studies on laboratory animals and people with hyperlipidaemia, a number of hypolipidaemic foods and beverages have been identified, of relevance to traditional chinese food culture. their absence from the diet may, in part account for the increasing prevalence of hyperlipidaemia i ... | 1996 | 24394619 |
| molecular endocrinology of oocyte growth and maturation in fish. | pituitary gonadotropins (gths) are of primary importance in triggering oocyte growth and maturation. however, the actions of gths are not direct, but are mediated by the ovarian production of steroidal mediators of oocyte growth (estradiol-17β) and maturation (maturation-inducing hormone, mih; 17α,20β-dihydroxy-4-pregnen-3-one, 17α,20β-dp in salmonid fishes; 17α,20β,21-trihydroxy-4-pregnen-3-one, 20β-s in sciaenid fishes). it is established that production of estradiol-17β and 17α,20β-dp by salm ... | 1993 | 24202455 |
| temporal changes in growth, condition and trophic niche in juvenile cyprinus carpio infected with a non-native parasite. | in host-parasite relationships, parasite prevalence and abundance can vary over time, potentially impacting how hosts are affected by infection. here, the pathology, growth, condition and diet of a juvenile cyprinus carpio cohort infected with the non-native cestode bothriocephalus acheilognathi was measured in october 2012 (end of their first summer of life), april 2013 (end of first winter) and october 2013 (end of second summer). pathology revealed consistent impacts, including severe compres ... | 2015 | 26394706 |
| first record of the invasive asian fish tapeworm bothriocephalus acheilognathi in honduras, central america. | this paper provides the first report of the invasive asian fish tapeworm, bothriocephalus acheilognathi yamaguti, 1934, in honduras. the cestode was found in profundulus portillorum (cyprinodontiformes: profundulidae), which represents a new host record, and which is a member of a genus faced with a variety of conservation challenges, now potentially complicated by the presence of this pathogenic cestode. nearly complete sequence data from the its-1 5.8s and its-2 regions corroborate the determi ... | 2015 | 25654444 |
| phenotypical and genotypical characterization of shewanella putrefaciens strains isolated from diseased freshwater fish. | between 2007 and 2012, a variety of disease outbreaks most often characterized by skin disorders were observed among different species of freshwater fish in poland. in most cases, the clinical signs included focally necrotized gills, necrotic skin lesions or ulcers. internally, haemorrhages, oedematous kidney and abnormal spleen enlargement were generally noted. the disorders were accompanied by increased mortality. most of the problems concerned cultured common carp cyprinus carpio l. and rainb ... | 2015 | 24552171 |
| introduced pathogens and native freshwater biodiversity: a case study of sphaerothecum destruens. | a recent threat to european fish diversity was attributed to the association between an intracellular parasite, sphaerothecum destruens, and a healthy freshwater fish carrier, the invasive pseudorasbora parva originating from china. the pathogen was found to be responsible for the decline and local extinction of the european endangered cyprinid leucaspius delineatus and high mortalities in stocks of chinook and atlantic salmon in the usa. here, we show that the emerging s. destruens is also a th ... | 2012 | 22615866 |
| zebrafish foxo3b negatively regulates antiviral response through suppressing the transactivity of irf3 and irf7. | forkhead box o (foxo)3, a member of the foxo family of transcription factors, plays key roles in various cellular processes, including development, longevity, reproduction, and metabolism. recently, foxo3 has also been shown to be involved in modulating the immune response. however, how foxo3 regulates immunity and the underlying mechanisms are still largely unknown. in this study, we show that zebrafish (danio rerio) foxo3b, an ortholog of mammalian foxo3, is induced by polyinosinic-polycytidyl ... | 2016 | 27815423 |
| zebrafish stat6 negatively regulates ifnφ1 production by attenuating the kinase activity of tank-binding kinase 1. | the aquatic spring viremia of carp virus (svcv) causes significant mortality in common carp (cyprinus carpio), and tbk1 plays a crucial role in the retinoic acid-inducible gene i (rig-i)-like receptor (rlr) system by phosphorylating its substrates to induce type i interferons (ifns) and cellular antiviral responses. in this study, we report that zebrafish stat6 is induced during svcv infection and reduces ifnφ1 expression by suppressing tbk1 phosphorylation. a typical ifn stimulatory response el ... | 2017 | 27743998 |
| molecular characterization, tissue distribution and expression, and potential antiviral effects of trim32 in the common carp (cyprinus carpio). | tripartite motif-containing protein 32 (trim32) belongs to the tripartite motif (trim) family, which consists of a large number of proteins containing a ring (really interesting new gene) domain, one or two b-box domains, and coiled coil motif followed by different c-terminal domains. the trim family is known to be implicated in multiple cellular functions, including antiviral activity. however, it is presently unknown whether trim32 of common carp (cyprinus carpio) has the antiviral effect. in ... | 2016 | 27735853 |