| effect of dickkopf-1 and colony stimulating factor-2 on the developmental competence, quality, gene expression and live birth rate of buffalo (bubalus bubalis) embryos produced by hand-made cloning. | a functional canonical wnt signaling pathway exists in preimplantation embryos and inhibits embryonic development. recent studies suggest that this pathway is over-expressed in nuclear transferred (nt), compared to ivf embryos. the present study investigated the effects of dickkopf-1 (dkk1), an inhibitor of canonical wnt signaling pathway and colony stimulating factor-2 (csf2), an embryokine, on the developmental competence, quality, gene expression and live birth rate of nt buffalo embryos prod ... | 2020 | 32823021 |
| semen parameters and fertility potency of a cloned water buffalo (bubalus bubalis) bull produced from a semen-derived epithelial cell. | semen contains epithelial cells that can be cultured in vitro. for somatic cell nuclear transfer applications, it is essential to know whether clone(s) produced from semen-derived epithelial cells (sedecs) are healthy and reproductively competent. in this study, the semen and fertility profile of a cloned bull (c1) that was produced from a sedec were compared with its donor (d1) and with two cloned bulls (c2, c3) that were produced from commonly used skin-derived fibroblast cells (skdfcs). we ob ... | 2020 | 32822364 |
| sequencing and assembly of the egyptian buffalo genome. | water buffalo (bubalus bubalis) is an important source of meat and milk in countries with relatively warm weather. compared to the cattle genome, a little has been done to reveal its genome structure and genomic traits. this is due to the complications stemming from the large genome size, the complexity of the genome, and the high repetitive content. in this paper, we introduce a high-quality draft assembly of the egyptian water buffalo genome. the egyptian breed is used as a dual purpose animal ... | 2020 | 32813723 |
| investigation of the role of aromatic residues in the antimicrobial peptide bucathl4b. | antimicrobial peptides (amps) are an attractive alternative to traditional small molecule antibiotics as amps typically target the bacterial cell membrane. a trp-rich peptide sequence derived from water buffalo (bubalus bubalis), bucathl4b was previously identified as a broad-spectrum antimicrobial peptide. | 2020 | 32798369 |
| seasonal effects on mirna and transcriptomic profile of oocytes and follicular cells in buffalo (bubalus bubalis). | season clearly influences oocyte competence in buffalo (bubalus bubalis); however, changes in the oocyte molecular status in relation to season are poorly understood. this study characterizes the microrna (mirna) and transcriptomic profiles of oocytes (oos) and corresponding follicular cells (fcs) from buffalo ovaries collected in the breeding (bs) and non-breeding (nbs) seasons. in the bs, cleavage and blastocyst rates are significantly higher compared to nbs. thirteen mirnas and two mrnas show ... | 2020 | 32782284 |
| remote monitoring system as a tool for calving management in mediterranean buffalo heifers (bubalus bubalis). | buffalo breeding is common in southern italy. dystocia compromises dam's and newborn health and welfare. difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. herein, we aimed to evaluate a remote calving alarm system in 15 mediterranean buffalo heifers. an intravaginal probe was placed close to the external cervical os once premonitory signs of delivery were observed. no vaginal discharge nor signs of dis ... | 2020 | 32780888 |
| escherichia coli membrane-derived oxygen-reducing enzyme system (oxyrase) protects bubaline spermatozoa during cryopreservation. | the objective of this study was to determine the effectiveness of deoxygenation of semen extender using escherichia coli membrane-derived oxygen scavenger (oxyrase) on post-thaw quality of buffalo (bubalus bubalis) spermatozoa. sixteen semen ejaculates, four each from four bulls, were each divided into five equal fractions, diluted using tris-egg yolk extender supplemented with different concentrations of oxyrase (0, 0.3, 0.6, 0.9, and 1.2 u/ml), designated as treatments t1, t2, t3, t4, and t5, ... | 2020 | 32780495 |
| theca cell-conditioned medium added to in vitro maturation enhances embryo developmental competence of buffalo (bubalus bubalis) oocytes after parthenogenic activation. | theca cells (tcs) play a key role in follicular growth and atresia. tcs synthesize androgens that act as substrate for granulosa cells (gcs) aromatization to estrogens needed for oocyte maturation. however, the effects of tcs in the form of conditioned medium on in vitro maturation (ivm) and developmental competence of buffalo oocytes remain unclear. in the present study, we examined the impacts of tc-conditioned medium (tccm) on maturation efficiency and embryo development of buffalo oocytes af ... | 2020 | 32767798 |
| feeding preferences and nutritional niche of wild water buffalo (bubalus arnee) in koshi tappu wildlife reserve, nepal. | the nutritional characteristics of food resources play an important role in the foraging behavior of animals and can provide information valuable to their conservation and management. we examined the nutritional ecology of wild water buffalo (bubalus arnee; hereafter "buffalo") in the koshi tappu wildlife reserve of nepal during autumn using a multidimensional nutritional niche framework. we identified 54 plant species as being foraged by buffalo. we found that buffalo consumed graminoids and fo ... | 2020 | 32760500 |
| theca cell-conditioned medium enhances steroidogenesis competence of buffalo (bubalus bubalis) granulosa cells. | theca cells (tcs) play a crucial role in follicular development and atresia. tcs synthesize the androgens that act as substrate for granulosa cells (gcs) aromatization to estrogens needed for follicular growth. however, the effects of tcs in the form of conditioned medium on steroidogenesis in buffalo gcs remain unclear. in the present study, the impacts of tc-conditioned medium (tccm) on estrogen synthesis in buffalo gcs were examined. the results showed that tcs secreted principally testostero ... | 2020 | 32748525 |
| diversity analysis at mhc class ii dqa locus in buffalo (bubalus bubalis) indicates extensive duplication and trans-species evolution. | variation at mhc class ii-dqa locus in riverine and swamp buffaloes (bubu) has been explored in this study. through sequencing of buffalo dqa, 48 nucleotide variants identified from 17 individuals, reporting 42 novel alleles, including one pseudogene. individual animal displayed two to seven variants, suggesting the presence of more than two bubu-dqa loci, as an evidence of extensive duplication. dn values were found to be higher than ds values at peptide binding sites, separately for riverine a ... | 2020 | 32738270 |
| expression pattern of glut 1, 5, 8 and citrate synthase transcripts in buffalo (bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo. | in vitro-produced (ivp) embryos are reported to be developmentally lesser competent than in vivo-derived (ivd) embryos and supposed to differ in the expression of genes related with glucose metabolism. so, the present study was conducted to analyse the expression pattern of glut 1, 5, 8 and citrate synthase (cs) in oocytes and embryos produced in vivo or in vitro in buffalo. ivd embryos were obtained from 18 superovulated buffaloes. ivp embryos were obtained from slaughterhouse-derived oocytes s ... | 2020 | 32706414 |
| molecular diagnosis and prevalence of trypanosoma vivax (trypanosomatida: trypanosomatidae) in buffaloes and ectoparasites in the brazilian amazon region. | trypanosoma vivax ziemann is a parasite that affects both wild and domestic ungulates and is transmitted mechanically via tabanids and other blood-sucking insects in the americas. a total of 621 blood samples from water buffaloes (bubalus bubalis (linnaeus) (artiodactyla: bovidae), and 184 ectoparasite samples (amblyomma cajennense (fabricius) sensu stricto and rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae), and haematopinus tuberculatus (burmeister) (phthiraptera: haematopin ... | 2020 | 32696974 |
| influence of uterine torsion in water buffaloes (bubalus bubalis) with insights into the hematological and biochemical prognostic values regarding to manual correction. | bovine uterine torsion refers to the rotation of the gravid uterus around its longitudinal axis. it is the dominant cause of dystocia in buffaloes. a total of two hundred and seventeen dystocia's egyptian buffaloes were examined, and another twenty were apparently normal at the full term (healthy pregnant group). from the dystocia's buffaloes, twenty-three percentage (n = 50) was uterine torsion after gynecology inspection at presentation (ut group) and 5 days after the manual correction (utc gr ... | 2020 | 32677006 |
| activation of wnt/β-catenin signaling pathway enhances the derivation of buffalo (bubalus bubalis) embryonic stem cell-like cells. | wnt/β-catenin signaling pathway plays an important role in maintaining self-renewal and pluripotency of human and mouse embryonic stem cells (escs). activation of wnt/β-catenin signaling pathway by glycogen synthase kinase-3 (gsk3) inhibitor, the wnt signaling agonist, could maintain the pluripotency of human and mouse escs in the presence of serum. however, the role of signaling pathway in the derivation of buffalo escs remains unclear. in this study, we used gsk3 inhibitors (6-bromoindirubin-3 ... | 2020 | 32673062 |
| early prediction of corpus luteum functionality using an imaging software. | the present study aimed to assess the applicability of luteal blood flow data acquired through the use of color doppler ultrasonography and a post-processing analysis tool (imagej) for predicting pregnancy in buffaloes (bubalus bubalis). the experiment was carried out on 59 multiparous italian mediterranean buffaloes that underwent synchronization of estrus and fixed-time artificial insemination (tai). corpus luteum features (size: cls and blood flow: bfa) were taken from day 5 to 10 after tai a ... | 2020 | 32626727 |
| passive immunity transfer in water buffaloes (bubalus bubalis). | this study aimed to evaluate passive immunity transfer in healthy buffalo calves. colostrum samples from heifers (without previous calving) and primiparous and pluriparous dams and blood samples from their offspring were obtained at calving, before colostrum intake, and at 24, 48, and 72 h after calving for determination of serum activities of gammaglutamyltransferase and alkaline phosphatase and serum concentrations of total protein (tp), immunoglobulin a (iga) and igg, and lactoferrin. the res ... | 2020 | 32626726 |
| effect of synergism between carboxylated poly-l-lysine and glycerol on freezability of nili-ravi buffalo (bubalus bubalis) semen. | carboxylated poly-l-lysine (cpll), an ampholytic polymer, has remarkable cryoprotective properties. it was hypothesized that cpll will reduce/replace glycerol in extender and improve the freezability of buffalo semen. the objective was to evaluate various combinations of cpll and glycerol in extenders for any synergism toward cryosurvivability of nili-ravi buffalo sperm. semen collected from four nili-ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based exte ... | 2020 | 32609000 |
| caffeic acid improves microscopic sperm parameters and antioxidant status of buffalo (bubalus bubalis) bull semen following freeze-thawing process. | this study investigates the effects of cryopreservation and supplementation of buffalo's semen with caffeic acid. it studies the effects of different caffeic acid concentrations on cryopreservation capacity of the buffalo and evaluates their influence on various sperm parameters like motility, viability, progressive motility, sperm plasma membrane integrity, and antioxidant status. twenty-four semen samples were collected with an artificial vaginal from three adult water buffalos. the semen samp ... | 2020 | 32590017 |
| first record of natural infection with anaplasma marginale in sucking lice infesting the water buffalo (bubalus bubalis) in mexico. | the water buffalo (bubalus bubalis) is an asian species of bovine which was introduced in mexico in 1992, as an alternative for milk and meat production. however, little is known about its associated ectoparasites, in particular about their lice. as a part of a project to identify the diversity and prevalence of anaplasma in cattle and water buffaloes in the state of veracruz, mexico, we visited a ranch in the downtown area in which 42 buffaloes were visually inspected for the presence of ectopa ... | 2020 | 32588174 |
| high trypanosoma vivax infection rates in water buffalo and cattle in the brazilian lower amazon. | highly sensitive and accurate molecular diagnostic methods have not yet been employed for livestock trypanosomosis in the brazilian lower amazon although the first reports of trypanosoma vivax and trypanosoma evansi in brazil were in water buffalo (bubalus bubalis) in this region. the present study assessed trypanosomosis in buffalo and cattle raised in communal and seasonally flooding pastures in the state of pará using the fluorescent fragment length barcoding (fflb) method. t. evansi was not ... | 2020 | 32565339 |
| extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes. | extracellular vesicles (evs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. the aim of the present report was to provide the first isolation, quantification and protein characterization of evs from buffalo (bubalus bubalis) sera infected with theileria spp. | 2020 | 32528536 |
| identification of pathogenic leptospira species in the urogenital tract of water buffaloes (bubalus bubalis) from the amazon river delta region, brazil. | in the current context of deforestation and fire in the amazon, buffaloes could be a cost-effective and sustainable alternative for cattle production in the region, as they can convert low-quality foods and be raised in floodplain areas. however, little is known about the reproductive diseases that affect these animals; thus, the purpose of this study was to perform the molecular characterization of leptospira spp. in the urogenital tract of water buffaloes (bubalus bubalis) raised in the amazon ... | 2020 | 32478114 |
| rumen fluke, fischoederius elongatus (trematoda: gastrothylacidae): preliminary investigation of suitable conditions for egg hatching. | various temperatures may have different effects on the distribution of paramphistomes that cause amphistomosis in cattle, including fischoederius elongatus. therefore, this study aimed to investigate the effects of different temperature treatments on f. elongatus hatching, with specific identification using morphological, histological, and phylogenetic analysis. all specimens were collected from two buffalo (bubalus bubalis) rumens in a slaughterhouse in pathum thani province, thailand. f. elong ... | 2020 | 32470871 |
| ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (bubalus bubalis) testis. | protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. although several ubiquitinated proteins have been found in buffalo (bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. in the present study, we first identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive ... | 2020 | 32469046 |
| evaluation and mining the applicable methods of roughage digestibility determination for buffalo (bubalus bubalis). | buffalo is an amazing ruminant with high tolerance to low-quality roughage. due to the special breeding environment, there are few reports on the digestibility of roughage in buffalo because it is difficult to quantify ingestion and egestion. to find more applicable method to determine the digestibility of low-quality roughage in buffalo, this study was conducted to compare total feces collection (tfc) method with the following three indirect techniques: cr2o3 (chromic oxide), aia (acid-insolubl ... | 2020 | 32458351 |
| effects of feeding and maturation system on qualitative characteristics of buffalo meat (bubalus bubalis). | we aimed to evaluate the effects of post dry ageing (pda) period on meat colour and rheological characteristics in 16 buffalo bulls fed two different diets: with (frs) or without (ctl) rye grass. animals were randomly divided into two feeding groups and slaughtered at 540 ± 4.7 and 533 ± 7.0 kg of live body weight, respectively, for the ctl and frs group. after five days post-mortem ageing (t0), semitendinosus muscle (st) and longissimus muscle (ld) underwent a prolonged maturation process in a ... | 2020 | 32455847 |
| generation of transgenic cloned buffalo embryos harboring the egfp gene in the y chromosome using crispr/cas9-mediated targeted integration. | sex control technology is of great significance in the production of domestic animals, especially for rapidly breeding water buffalo (bubalus bubalis), which served as a research model in the present study. we have confirmed that a fluorescence protein integrated into the y chromosome is fit for sexing pre-implantation embryos in the mouse. firstly, we optimized the efficiency of targeted integration of exogenous gene encoding enhanced green fluorescent protein (egfp) and mcherry in neuro-2a cel ... | 2020 | 32426378 |
| sequencing of lipoprotein lipase gene in the mediterranean river buffalo identified novel variants affecting gene expression. | lipoprotein lipase (lpl) is a key enzyme for lipid metabolism, playing a fundamental role in the composition of fat in adipose tissue and milk. the lpl gene has been seldom investigated in dairy ruminants and barely studied in river buffalo (bubalus bubalis). the aim of this work was to explore the genetic diversity of lpl and its promoter and to identify functional mutations, using a combined approach based on sequencing, dual-color electrophoretic mobility shift assay, and quantitative pcr. th ... | 2020 | 32418698 |
| the addition of resveratrol in tris citric acid extender ameliorates post-thaw quality parameters, antioxidant enzymes levels, and fertilizing capability of buffalo (bubalus bubalis) bull spermatozoa. | resveratrol is a natural grape-derived polyphenol with potent antioxidant properties that protect spermatozoa against lipid peroxidation (lpo) by eradicating free radicals. the objectives of this study were to 1) appraise the effects of resveratrol in extender on post-thaw quality parameters, antioxidant enzymes, adenosine triphosphate (atp), dna fragmentation, lpo and 2) fertilizing capability of buffalo bull spermatozoa. semen was collected from four fertility proven bulls with artificial vagi ... | 2020 | 32388038 |
| genomic identification, evolution, and expression analysis of collagen genes family in water buffalo during lactation. | collagens, as extracellular matrix proteins, support cells for structural integrity and contribute to support mammary basic structure and development. this study aims to perform the genomic identification, evolution, and expression analyses of the collagen gene family in water buffalo (bubalus bubalis) during lactation. a total of 128 buffalo collagen protein sequences were deduced from the 45 collagen genes identified in silico from buffalo genome, which classified into six groups based on thei ... | 2020 | 32384775 |
| the domesticated buffalo - an emerging model for experimental and therapeutic use of extraembryonic tissues. | large animals play important roles as model animals for biomedical sciences and translational research. the water buffalo (bubalus bubalis) is an economically important, multipurpose livestock species. important assisted reproduction techniques, such as in vitro fertilization, cryo-conservation of sperm and embryos, embryo transfer, somatic cell nuclear transfer, genetic engineering, and genome editing have been successfully applied to buffaloes. recently, detailed whole genome data and transcri ... | 2020 | 32320839 |
| multivariate analysis of body morphometric traits in conjunction with performance of reproduction and milk traits in crossbred progeny of murrah × jafarabadi buffalo (bubalus bubalis) in north-eastern brazil. | we studied the relationship between body morphometric traits, and their underlying association with milk production (mp), lactation length (ll), first calving interval (fci) and subsequent calving interval (ci) of crossbred progeny of murrah × jafarabadi buffalo aiming to assist in selection programs. we carried out principal component analysis (pca) of the body morphometric traits, which include breast width (bw), thigh width (thw), hip width (hw), rump width (rw), rump length (rl), body depth ... | 2020 | 32315344 |
| abatement of enteric methane production from lactating murrah buffaloes (bubalus bubalis) with improving production performance and immune status through dietary supplementation of composite feed additive. | ruminant livestock production processes are the major sources of methane production in agriculture sector triggering global environmental pollution. above 90% of world buffalo population present in asian countries, india ranks first and contributes significantly to the environmental pollution by enteric methane emissions. in this study, we examined the effect of dietary composite feed additive supplementation on ruminal methane production, nutrient utilization, milk production and immune status ... | 2020 | 32314293 |
| high prevalence of subclinical paratuberculosis in buffaloes (bubalus bubalis) in maranhão, brazil. | paratuberculosis is an infectious, chronic, and incurable disease that affects ruminants, causing enteritis and chronic granulomatous lymphadenitis, characterized by malabsorption syndrome, its agent is the mycobacterium avium subsp. paratuberculosis (map). thus, the objective of this work was to identify and characterize map in buffalo herds slaughtered in baixada maranhense region. samples of intestines, mesenteric lymph nodes, and ileocecal valves were collected from 115 buffaloes slaughtered ... | 2020 | 32291741 |
| efficient transovarial transmission of babesia spp. in rhipicephalus microplus ticks fed on water buffalo (bubalus bubalis). | water buffaloes can be infected by tick-borne pathogens (tbps) in endemic areas where cattle and buffalo coexist. among tbps affecting buffaloes is the apicomplexan hemoparasites babesia bovis and b. bigemina, transmitted by rhipicephalus microplus ticks. however, little empirical evidence exists on whether buffalo can support tbps' infection and transmission. a cohort study was designed to measure the infestation levels of r. microplus in buffaloes as well as the ability of buffalo-fed ticks to ... | 2020 | 32290468 |
| mitochondrial sequence-based evolutionary analysis of riverine-swamp hybrid buffaloes of india indicates novel maternal differentiation and domestication patterns. | in this study, mitochondrial d-loop sequence data on riverine, swamp and hybrid buffaloes from india have been generated and compared with other reported indian riverine, chinese and bangladeshi swamp buffalo populations. sequence analysis revealed the presence of 132 haplotypes, with a haplotype diversity of 0.9611 ± 0.0045 and a nucleotide diversity of 0.04801 ± 0.00126. for the first time, the existence of riverine-swamp hybrids among the indian chilika buffalo population has been recorded, h ... | 2020 | 32281135 |
| correction to: genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in iranian river buffalo, bubalus bubalis. | following publication of the original article [1], the authors flagged that the article had published with the author 'ali jalil sarghale' erroneously omitted from the author list. | 2020 | 32268878 |
| recombinant vaccine against botulism in buffaloes: evaluation of the humoral immune response over 12 months. | botulism is a neuroparalytic intoxication, usually fatal, caused by the botulinum toxins (bonts). vaccination is the best-known strategy to prevent this disease in ruminants. serotypes c and d and their variants cd and dc are the main types responsible for botulism in bovine and buffaloes in brazil and cattle in japan and europe. brazil has a herd of approximately 1.39 million buffaloes and is the largest producer in the western world. this study aimed to assess the humoral immune response of bu ... | 2020 | 32247696 |
| seroprevalence and clinical outcomes of neospora caninum, toxoplasma gondii and besnoitia besnoiti infections in water buffaloes (bubalus bubalis). | one hundred twenty-four water buffaloes (bubalus bubalis) originating from 9 farms located in southern italy were tested to investigate simultaneously, for the first time, the seroprevalence of the protozoa neospora caninum, toxoplasma gondii and besnoitia besnoiti by elisa tests and to evaluate the clinical findings potentially associated to the presence of these aborting parasitic pathogens. twenty-five of 124 buffaloes (20.2%) were positive for n. caninum, while 17/124 (13.7%) for t. gondii. ... | 2020 | 32235734 |
| milk somatic cell count and its relationship with milk yield and quality traits in italian water buffaloes. | in southern italy, buffalo (bubalus bubalis) milk is mostly intended for the manufacture of mozzarella di bufala campana protected denomination of origin (pdo) cheese. despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare. milk somatic cell count (scc) is used worldwide as an indicator of udder health in individual milk an ... | 2020 | 32229124 |
| evaluation of the middle ear in water buffalos (bubalus bubalis) by gross anatomy and cone beam computed tomography. | the purpose of this study was to provide a description of gross middle ear morphology in water buffalos, augmented with additional data on the osseous structures of middle ear derived from cone-beam computed tomography (cbct). | 2020 | 32207849 |
| poly i:c stimulation in-vitro as a marker for an antiviral response in different cell types generated from buffalo (bubalus bubalis). | the innate immune system is activated upon virus invasion of a host cell by recognizing viral component, such as dsrna through specific receptors, resulting in the production of type- i ifns, which confer an antiviral state within the invaded as well as surrounding cells. in the present study, fibroblast, monocyte and macrophage cells derived from water buffalo (bubalus bubalis) were exposed to a synthetic dsrna analogue, poly i:c to mimic viral invasion in each cell type. recognition of poly i: ... | 2020 | 32200171 |
| in-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation. | the mature mammary gland is made up of a network of ducts that terminates in alveoli. the innermost layer of alveoli is surrounded by the differentiated mammary epithelial cells (mecs), which are responsible for milk synthesis and secretion during lactation. however, the mecs are in a state of active proliferation during pregnancy, when they give rise to network like structures in the mammary gland. buffalo (bubalus bubalis) constitute a major source of milk for human consumption, and the mecs a ... | 2020 | 32179766 |
| first description of giardia duodenalis in buffalo calves (bubalus bubalis) in southwest region of são paulo state, brazil. | we performed molecular characterization of giardia duodenalis in buffalo calves from the southwest region of são paulo state, brazil. a total of 183 fecal samples of murrah breed buffaloes up to six months of age were collected. we examined these samples by the polymerase chain reaction (pcr) targeting the small-subunit ribosomal rna gene and positive samples were characterized using additional pcr assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phospha ... | 2019 | 32095632 |
| genomic analyses reveal distinct genetic architectures and selective pressures in buffaloes. | the domestic buffalo (bubalus bubalis) is an essential farm animal in tropical and subtropical regions, whose genomic diversity is yet to be fully discovered. | 2020 | 32083286 |
| glutathione-s-transferase: an important diagnostic antigen of liver amphistome gigantocotyle explanatum, infecting the indian water buffalo. | the foodborne trematodiases pose a significant health problem to the animals as well as the human population living in close proximities with the livestock and are still considered as the neglected tropical diseases by the world health organisation. the digenetic trematode, gigantocotyle explanatum infecting the liver of indian water buffalo, bubalus bubalis, has been identified as one of the most common helminth parasite responsible for the disease, amphistomosis, in livestock. despite huge aba ... | 2020 | 32081660 |
| genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in iranian river buffalo, bubalus bubalis. | consecutive homozygous fragments of a genome inherited by offspring from a common ancestor are known as runs of homozygosity (roh). roh can be used to calculate genomic inbreeding and to identify genomic regions that are potentially under historical selection pressure. the dataset of our study consisted of 254 azeri (az) and 115 khuzestani (khz) river buffalo genotyped for ~ 65,000 snps for the following two purposes: 1) to estimate and compare inbreeding calculated using roh (froh), excess of h ... | 2020 | 32041535 |
| detection of polymorphism within leptin gene in egyptian river buffalo and predict its effects on different molecular levels. | leptin (lep) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. the present investigation studied the polymorphism in lep gene which is related to fertility in 81 female egyptian river buffalo. | 2020 | 32037476 |
| investigating genetic variability in hsp70 gene-5'-fragment and its association with thermotolerance in murrah buffalo (bubalus bubalis) under sub-tropical climate of india. | the present study was undertaken to investigate genetic variability in a fragment comprising 5'utr along with partial coding sequence of hsp70 gene and its association with thermotolerance traits in murrah buffalo at icar-research complex for eastern region, patna (india). the allelic variants were identified from genomic dna samples using sscp technique. the pcr products were sequenced and analyzed. data on different thermotolerance traits recorded in three seasons were analyzed by least square ... | 2020 | 32020511 |
| embryo transfer in buffalo (bubalus bubalis). | the use of assisted reproductive technologies, such as superovulation and in vivo embryo production and in vitro embryo production (ivep), has increased rapidly in recent years and is now applied worldwide for genetic improvement in beef and dairy buffaloes. although in vivo embryo production has been shown to be feasible in buffalo, low efficiency and limited commercial application has been documented. these results could be associated with low antral follicle populations, high levels of follic ... | 2020 | 31996292 |
| asian water buffalo: domestication, history and genetics. | the domestic asian water buffalo (bubalus bubalis) is found on all five continents, with a global population of some 202 million. the livelihoods of more people depend on this species than on any other domestic animal. the two distinct types (river and swamp) descended from different wild asian water buffalo (bubalus arnee) populations that diverged some 900 kyr bp and then evolved in separate geographical regions. after domestication in the western region of the indian subcontinent (ca. 6300 ye ... | 2020 | 31967365 |
| effects of treatment with a microrna mimic or inhibitor on the developmental competence, quality, epigenetic status and gene expression of buffalo (bubalus bubalis) somatic cell nuclear transfer embryos. | expression levels of 13 micrornas (mirnas) were compared between buffalo blastocysts produced by somatic cell nuclear transfer through hand-made cloning and ivf to improve cloning efficiency. expression of mir-22, mir-145, mir-374a and mir-30c was higher, whereas that of mir-29b, mir-101, mir-302b, mir-34a, mir-21 and mir-25 was lower, in nuclear transferred (nt) than ivf embryos; the expression of mir-200b, mir-26a and mir-128 was similar between the two groups. based on these, mir-145, which i ... | 2020 | 31959280 |
| local peritonitis and omental bursitis as sequelae of abomasal ulceration in buffaloes (bubalus bubalis): clinical findings and outcome of treatment. | abomasal ulcers are difficult to diagnose clinically with limited therapeutic approach to combat the disease. omental bursitis (ob) and local peritonitis (lp) are known sequelae of abomasal ulcer in cows. in this study, differentiation between ob and lp in regard to clinical symptoms, biochemical analyses and the response to treatment in buffaloes was done. twenty buffaloes (bubalus bubalis) were admitted with a history of intermittent appetite, wasting and mild abdominal distension during the p ... | 2020 | 31955661 |
| relationship between angioarchitecture of the testicular artery and spermiogram parameters in egyptian buffalo bulls (bubalus bubalis). | the current study aimed to investigate the effect of testicular artery angioarchitecture on the spermiogram parameters in egyptian buffalo bulls. eight adult buffalo bulls aged between 2 and 8 years were used for semen evaluation. for anatomical studies, the masculine gonads were collected after slaughtering 30 adult bulls and prepared for injection by different masses (urographine® , latex and epoxy) through the testicular artery. the mass activity of the ejaculate was assessed immediately afte ... | 2020 | 31944422 |
| effects of different culture systems on the culture of prepuberal buffalo (bubalus bubalis) spermatogonial stem cell-like cells in vitro. | currently, the systems for culturing buffalo spermatogonial stem cells (sscs) in vitro are varied, and their effects are still inconclusive. in this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (knockout serum replacement) materials on the in vitro culture of buffalo ssc-like cells. significantly more ddx4- and uchl1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined m ... | 2020 | 31940692 |
| normal anatomic reference of pastern and coffin joints in egyptian buffalo (bubalus bubalis): a compared atlas of cross-sectional anatomy, magnetic resonance imaging and computed tomography. | the purpose of this study was to describe normal magnetic resonance imaging and computed tomographic anatomy of pastern and coffin joints in egyptian buffalo using cadaveric distal limbs. this study was achieved using twelve fresh cadaveric distal limbs from adult healthy buffaloes of both sexes. these cadaveric limbs were scanned using a 1 tesla mri scanner and ct scanner, injected with red latex, frozen at -20°c for 1 week, and then sectioned into sagittal, dorsal and transverse slices. the ob ... | 2020 | 31910304 |
| polymorphisms of the kappa casein (csn3) gene and inference of its variants in water buffalo (bubalus bubalis). | kappa casein plays a crucial role in the formation of stable casein micelles and has a key influence on milk-clotting properties. however, current understanding of buffalo csn3 gene polymorphisms is not sufficient. in this study, the polymorphisms in the complete coding sequence (cds) of the buffalo csn3 were detected using pcr product direct sequencing. the cds of csn3 for river and swamp buffalo was the same in length, which contained an open reading frame of 573 nucleotides encoding a peptide ... | 2019 | 31893216 |
| effect of carboxylated poly l-lysine as a cryoprotectant on post-thaw quality and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull semen. | buffalo bull sperm are more prone to cryo-injuries. glycerol being the most common permeable cryoprotectant exerts cytotoxic effects on sperm which cause a reduction in fertility. thus, the exploration of new cryoprotectant is needed. for this purpose, we investigated the effect of carboxylated poly l-lysine (cpll) as cryoprotectant used with different concentrations of glycerol on post-thaw sperm motility, kinematics, plasma membrane integrity, mitochondrial membrane potential (mmp), lipid pero ... | 2020 | 31884337 |
| dose-dependent effect of romifidine on intraocular pressure in clinically healthy buffalo (bubalus bubalis). | in the present study, changes in intraocular pressure (iop) associated with romifidine sedation in buffalo were evaluated. eighteen healthy adult, non-pregnant, buffalo without ocular abnormalities were used in a prospective randomized trial. buffalo were allocated into three groups (six each). buffalo in the treated groups received an intramuscular injection (im) of romifidine at 40 or 50 μg/kg. the control group was administrated an equivalent volume of sterile saline (0.9% nacl; 0.4 ml/100 kg ... | 2019 | 31867457 |
| expression and functional role of fibroblast growth factors (fgf) in placenta during different stages of pregnancy in water buffalo (bubalus bubalis). | the present study documented the expression and functional role of fibroblast growth factors (fgfs) family and their receptors (fibroblast growth factor receptor, fgfrs) in placenta (cotyledon; cot, caruncle; car) during different stages of pregnancy in water buffalo. samples were collected from early pregnancy 1 (ep1); early pregnancy 2 (ep2); mid pregnancy (mp) and late pregnancy (lp) while diestrus stage of oestrus cycle (np) was taken as control. in addition, modulatory role of fgf2 on mrna ... | 2020 | 31864010 |
| unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review. | fibrin biopolymers, previously referred as "fibrin glue" or "fibrin sealants", are natural biomaterials with diverse applications on health. they have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. in the 1990s, to overcome th ... | 2019 | 31839802 |
| global transcriptome analysis of different stages of preimplantation embryo development in river buffalo. | water buffalo (bubalus bubalis) are divided into river buffalo and swamp buffalo subspecies and are essential livestock for agriculture and the local economy. studies on buffalo reproduction have primarily focused on optimal fertility and embryonic mortality. there is currently limited knowledge on buffalo embryonic development, especially during the preimplantation period. assembly of the river buffalo genome offers a reference for omics studies and facilitates transcriptomic analysis of preimp ... | 2019 | 31824777 |
| a new three-dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells. | at present, many three-dimensional (3d) culture systems have been reported, improving the oocyte quality of in vitro maturation (ivm), yet the mechanism still needs to be further explored. here we examined the effects of a new self-made 3d glass scaffold on buffalo oocyte maturation; meanwhile, the underlying mechanism on buffalo oocyte maturation was also detected. compared to the two-dimensional (2d) glass dish culture, results revealed that the 3d culture can improve the first polar body rate ... | 2020 | 31816136 |
| expression and localization of cellular flice-like inhibitory protein (cflip), an anti-apoptotic factor, in corpora lutea during the estrous cycle and pregnancy in thai swamp buffalo <i>(bubalus bubalis)</i>. | in female mammals, luteal cells rapidly proliferate and form corpora lutea (cls) after ovulation. the corpus luteum (cl) plays crucial roles in establishing and maintaining pregnancy. to gain further insights into the role of cellular flice-like inhibitory protein (cflip), an anti-apoptosis factor that is structurally similar to procaspase-8 but lacks proteolytic enzyme activity, we examined the expression in cls of thai swamp buffalos (bubalus bubalis) during the early, mid, and late stage of t ... | 2020 | 31801913 |
| sensitivity of buffaloes (bubalus bubalis) to heat stress. | based on ten years of data (2001-10), consisting of 12 673 observations on fortnightly milk yield of buffaloes reared in a dairy farm located in the northern sub-tropics (29°41'0″n, 76°59'0″e), the present study establishes the relationship between weather conditions and production performance of lactating buffaloes. the critical threshold level of maximum temperature-humidity index (thi) was estimated to be 74, which is higher than that of crossbred cows. the duration of discomfort period for b ... | 2019 | 31787123 |
| evaluation of a bovine cathelicidin elisa for detecting mastitis in the dairy buffalo: comparison with milk somatic cell count and bacteriological culture. | a recently developed bovine cathelicidin (cath) elisa was evaluated in the dairy buffalo (bubalus bubalis) by testing 618 quarter milk samples from a herd with subclinical mastitis cases. somatic cell count (scc) and bacteriological culture (bc) were carried out on the same samples for comparison. out of 618 quarters, 258 (41.75%) were positive to cath, 289 (46.76%) had scc > 200,000 cells/ml, and 457 (73.95%) were positive to bc. the most prevalent microorganism was staphylococcus aureus (sau, ... | 2020 | 31783263 |
| supplementation of l-tryptophan (an aromatic amino acid) in tris citric acid extender enhances post-thaw progressive motility, plasmalemma, mitochondrial membrane potential, acrosome, and dna integrities, and in vivo fertility rate of buffalo (bubalus bubalis) bull spermatozoa. | the aromatic amino acid l-tryptophan is an essential and versatile molecule, acts by transferring an electron to free radicals and protects the plasma membrane from injuries. the aim of the present study was to investigate the effects of l-tryptophan in extender on semen quality parameters, in vitro longevity and in vivo fertility rate of buffalo spermatozoa during cryopreservation. two ejaculates were collected from each bull (n = 2 ejaculates and n = 4 bulls) with artificial vagina at 42 °c fo ... | 2020 | 31783000 |
| unusual interplay of contrasting selective pressures on β-defensin genes implicated in male fertility of the buffalo (bubalus bubalis). | the buffalo, despite its superior milk-producing ability, suffers from reproductive limitations that constrain its lifetime productivity. male sub-fertility, manifested as low conception rates (crs), is a major concern in buffaloes. the epididymal sperm surface-binding proteins which participate in the sperm surface remodelling (ssr) events affect the survival and performance of the spermatozoa in the female reproductive tract (frt). a mutation in an epididymal secreted protein, beta-defensin 12 ... | 2019 | 31771505 |
| cross comparison of seminal plasma proteins from cattle and buffalo (bubalus bubalis). | the objective of this study was to evaluate seminal plasma proteins from cattle and buffalo (bubalus bubalis), to identify differences between related species. sixteen buffaloes and 16 cattle between 30 and 60 months of age were used. semen collection was performed by electroejaculation, followed by macroscopic and microscopic subjective analyses. after analysis, the samples were centrifuged at 800 g for 10 min, and the supernatant (seminal plasma) was recentrifuged at 10,000 g for 30 min at 4°c ... | 2020 | 31733131 |
| supplementation of green tea extract (gte) in extender improves structural and functional characteristics, total antioxidant capacity and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa. | the aim of this study was to elucidate the beneficial effects of green tea extract (gte) in tris citric acid extender on post thaw structural and functional characteristics, dna fragmentation (%), total antioxidant capacity (tac, μm/l), lipid peroxidation (lpo, μm/ml) levels and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa. gte is a acknowledged natural antioxidant, act as a free radical scavenger and protects spermatozoa against oxidative stress. three mature and donor buffal ... | 2020 | 31732163 |
| successful transplantation of transfected enriched buffalo (bubalus bubalis) spermatogonial stem cells to homologous recipients. | genetic modification of spermatogonial stem cells (sscs) is an alternative method to pronuclear microinjection and somatic cell nuclear transfer for transgenesis in large animals. in the present study, we optimized the process of homologous ssc transplantation in the water buffalo (bubalus bubalis) using transfected enriched sscs generated by a non-viral transfection approach. firstly, the ssc enrichment efficiencies of extracellular matrix components viz. collagen, gelatin, and datura stramoniu ... | 2020 | 31711692 |
| proteomic changes in the milk of water buffaloes (bubalus bubalis) with subclinical mastitis due to intramammary infection by staphylococcus aureus and by non-aureus staphylococci. | subclinical mastitis by staphylococcus aureus (sau) and by non-aureus staphylococci (nas) is a major issue in the water buffalo. to understand its impact on milk, 6 quarter samples with >3,000,000 cells/ml (3 sau-positive and 3 nas-positive) and 6 culture-negative quarter samples with <50,000 cells/ml were investigated by shotgun proteomics and label-free quantitation. a total of 1530 proteins were identified, of which 152 were significantly changed. sau was more impacting, with 162 vs 127 diffe ... | 2019 | 31676851 |
| genome-wide association studies for growth traits in buffaloes using the single step genomic blup. | growth traits are important for the profitability of buffalo breeding systems, since in general, these animals are raised both for meat and milk. in this study, the single-step genomic blup method was employed to prospect the genomic regions' associated with weight at standard ages of 100, 210, 365, and 550 days in a buffalo population, aiming to identify genes with stronger expression for those characteristics. we found 6, 1, 2, and 5 snps significantly associated (p value < 10-5) with weight a ... | 2020 | 31673966 |
| genetic profiling of hsp70 gene in murrah buffalo (bubalus bubalis) under sub-tropical climate of india. | this study was aimed to genetic profiling of heat shock protein 70 (hsp70) gene in murrah buffalo investigating 50 unrelated adult animals at icar-research complex for eastern region, patna (india) in winter, spring, and summer. pcr ready genomic dna samples and season-wise total rna samples were prepared. the pcr products of hsp70 eluted from agarose gel were sequenced and analyzed. the first-strand cdna was synthesized and concentration was equalized to 25 ng/μl. expression kinetics of mrna tr ... | 2019 | 31642046 |
| prevalence of eimeria species in water buffaloes (bubalus bubalis) from egypt and first report of eimeria bareillyi oocysts. | coccidiosis is a common disease of livestock, including water buffaloes. reports discussing the prevalence and identity of coccidian parasites in water buffaloes from egypt are few, and nothing is known concerning the most pathogenic species, eimeria bareillyi. fresh feces collected from 175 water buffaloes in dakahlia governorate, egypt, were processed using the standard flotation technique. eimerian oocysts were found in 49 (28%) samples, and 10 species were identified. eimeria bareillyi oocys ... | 2019 | 31599697 |
| multiple domestication of swamp buffalo in china and south east asia. | the domestication of swamp buffalo (bubalus bubalis carabanesis) has been discussed for years and still remained unclear. to obtain a better understanding of where, when and how the swamp buffaloes were domesticated, the complete mitochondrial dna d-loop sequences of 1,788 individuals from china, vietnam, laos, thailand, burma, bangladesh and india were investigated. our results revealed swamp buffalo with abundant genetic diversity. the lineage sa of swamp buffalo may be first domesticated in l ... | 2020 | 31588618 |
| high-throughput rna sequencing reveals ndufc2-as lncrna promotes adipogenic differentiation in chinese buffalo (bubalus bubalis l). | the buffalo (bubalus bubalis.l) is prevalent in china and the increasing demand for meat production has changed its role from being a beast of burden to a meat source. the low fat deposition level has become one of the main barriers for its use in meat production. it is urgent to reveal factors involved in fat deposition in buffalo. this study performed rna sequencing to investigate both long noncoding rnas (lncrnas) and mrnas of adipose tissues in young and adult buffalos. a total of 124 lncrna ... | 2019 | 31500202 |
| expression and functional role of bone morphogenetic proteins (bmps) in placenta during different stages of pregnancy in water buffalo (bubalus bubalis). | the objective of this study was to document the expression and functional role of bmps in the placental (caruncle; car, cotyledon; cot) during different stages of pregnancy in water buffalo. samples collected from early pregnancy 1 (ep1); early pregnancy 2 (ep2), mid pregnancy (mp), late pregnancy (lp) while the third stage of oestrus cycle (np) was taken as control. also, the synergistic role of bmp4/bmp7 or combination on mrna expression of vwf, pcna, star, cyp11a1, 3βhsd, and bax were studied ... | 2020 | 31445010 |
| clinical and ultrasonographic investigations of 30 water buffaloes (bubalus bubalis) with hepatomegaly. | knowledge of normal ultrasonographic dimensions of the liver and associated vascular structures is an important indicator for the diagnosis of hepatic diseases. enlargement of the liver beyond its normal dimensions is the term of hepatomegaly and ultrasonography is the primary and the suitable diagnostic technique for this condition. therefore, this study aimed to describe the clinical and ultrasonographic findings of liver diseases causing hepatomegaly in 30 buffaloes as well as to provide a ra ... | 2019 | 31439995 |
| a gene expression atlas of the domestic water buffalo (bubalus bubalis). | the domestic water buffalo (bubalus bubalis) makes a major contribution to the global agricultural economy in the form of milk, meat, hides, and draught power. the global water buffalo population is predominantly found in asia, and per head of population more people depend upon the buffalo than on any other livestock species. despite its agricultural importance, there are comparatively fewer genomic and transcriptomic resources available for buffalo than for other livestock species. we have gene ... | 2019 | 31428126 |
| expression of kisspeptin and its receptor in the hypothalamus of cyclic and acyclic buffalo (bubalus bubalis). | kisspeptin (kiss1), neurokinin-b (nkb) and dynorphin (dyn) neurons regulate the surge and pulsatile centres of gonadotropin releasing hormone (gnrh) in the hypothalamus and are modulated by the ovarian steroids. accordingly, we studied the temporospatial expression of kiss1, its receptor and other genes that regulate gnrh in the preoptic area (poa) and arcuate (arc) regions of hypothalamus at different phases of bubaline estrous cycle. brain of buffalo (n = 32) was collected immediately after ex ... | 2019 | 31419703 |
| differential expression of endometrial toll-like receptors (tlrs) and antimicrobial peptides (amps) in the buffalo (bubalus bubalis) with endometritis. | toll like receptors (tlrs) and β-defensins expressed in the endometrium are part of the innate uterine defense mechanism (udm). in the present study, transcriptional profile of tlrs (1-3, 6-8, 10, and) and β-defensins such as lingual antimicrobial peptide (lap), tracheal antimicrobial peptide (tap) and bovine neutrophil beta-defensin 4 (bnbd4) were studied. bubaline genitalia were collected from abattoir and the endometrium was categorized into one of the following seven groups (n = 7/group) bas ... | 2019 | 31407222 |
| reducing the cytoplasmic volume during hand-made cloning adversely affects the developmental competence and quality, and alters relative abundance of mrna transcripts and epigenetic status of buffalo (bubalus bubalis) embryos. | hand-made cloning (hmc) is a method of choice for somatic cell nuclear transfer (scnt). there is 20% to 50% of cytoplasm lost during manual enucleation of oocytes with hmc. to compensate, two enucleated demicytoplasts, instead of one, are fused with each donor cell, which leads to cytoplasm pooling from two different demicytoplasts. in this study, effects of using one, instead of two demicytoplasts (controls) was examined, for production of embryos using hmc. use of one demicytoplast decreased b ... | 2019 | 31405474 |
| abundance of adiponectin mrna transcript in the buffalo corpus luteum during the estrous cycle and effects on progesterone secretion in vitro. | adiponectin is an adipocyte derived cytokine implicated in energy homeostasis, insulin resistance and is involved in the regulation of reproduction both centrally and peripherally in animals. the present study was conducted to investigate adiponectin (adipoq) and its receptors adipor1 and adipor2 abundance of mrna transcript and protein in different stages of corpora lutea (cl) development during the estrous cycle of water buffalo and to determine the effect of adiponectin on cultured luteal cel ... | 2019 | 31405469 |
| polymorphisms in major histocompatibility complex genes and its associations with milk quality in murrah buffaloes. | animal breeding programs have used molecular genetic tools as an auxiliary method to identify and select animals with superior genetic merit for milk production and milk quality traits as well as disease resistance. genes of the major histocompatibility complex (mhc) are important molecular markers for disease resistance that could be applied for genetic selection. the aim of this study was to identify single nucleotide polymorphisms (snps) and haplotypes in drb2, drb3, dma, and dmb genes in mur ... | 2020 | 31385169 |
| superstimulation prior to the ovum pick-up improves the in vitro embryo production in nulliparous, primiparous and multiparous buffalo (bubalus bubalis) donors. | the aim of this study was to evaluate the ovarian follicular population, the oocyte yield and the in vitro embryo production (ivep) of nulliparous (nu), primiparous (pr) and multiparous (mu) buffalo donors submitted to the superstimulation with fsh prior to the ovum pick-up (opu). a total of 54 buffalo donors (18 nu, 15 pr and 21mu) received an intravaginal progesterone device (1.0 g) plus estradiol benzoate [2.0 mg, intramuscular (im)] at random stage of the estrous cycle (day 0) during the bre ... | 2019 | 31374459 |
| expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo. | adiponectin is an adipocyte-derived hormone regulating energy metabolism, insulin sensitivity and recently found to regulate reproduction. the current study was carried out to investigate gene and protein expression, immunolocalization of adiponectin and its receptors adipor1 and adipor2 in ovarian follicles of different developmental stages in water buffalo (bubalus bubalis) and to investigate the effect of adiponectin on steroid production in cultured bubaline granulosa cells. qpcr, western bl ... | 2019 | 31339602 |
| bioinformatics analysis of candidate genes for milk production traits in water buffalo (bubalus bubalis). | water buffalo (bubalus bubalis) is an important livestock species in developing countries due to its contribution to meat, milk production, and a certain form of labor. however, the genetic potential of buffalo milk production traits has not been fully exploited. to date, 516 candidate genes associated with milk production traits of buffalo have been identified. the present study aimed to explore the possible molecular mechanisms underlying milk production traits of this species through function ... | 2020 | 31321660 |
| isolation and identification of bubaline herpesvirus 1 (buhv-1) from latently infected water buffalo (bubalus bubalis) from iran. | in order to isolate buffaloes herpesvirus 1 (buhv-1) from latently infected water buffalo (bubalus bubalis), 16 buffalo heifers were selected from a herd. at first, animals were bled and their sera were tested by virus neutralization (vn) test, using bovine herpesvirus 1 (bohv-1). according to the results of vn test and dexamethasone injection (0.1 mg/kg bw) for 5 consecutive days, the examined buffaloes were divided into 4 groups. vaginal and nasal swabs were daily collected from all buffaloes ... | 2020 | 31313017 |
| toxoplasma gondii in water buffaloes (bubalus bubalis) from romania: what is the importance for public health? | the purpose of our study was to evaluate the prevalence of toxoplasma gondii infection in autochthonous carpathian buffaloes from northwestern romania by serology, pcr techniques, and mouse bioassay. agreement between mat and elisa, correlation between indirect and direct detection methods, and risk factors were evaluated. the apparent overall seroprevalence of t. gondii was 8.1% by mat and 6.6% by elisa. the agreement between elisa and mat was fair. the apparent seroprevalence was significantly ... | 2019 | 31309289 |
| rna sequencing and transcriptome analysis of buffalo (bubalus bubalis) blastocysts produced by somatic cell nuclear transfer and in vitro fertilization. | across farm animal species, the live birth rate obtained with somatic cell nuclear transfer (scnt) embryos is only <2% compared with >40% obtained with in vitro fertilization (ivf) embryos, primarily due to incomplete nuclear reprogramming which results in aberrant embryonic gene expression. we used rna sequencing to compare the global transcriptome profile of scnt and ivf buffalo blastocysts. scnt blastocysts expressed 17,061 transcripts, of which 941 were unique whereas, ivf blastocysts expres ... | 2019 | 31304661 |
| an objective volumetric method for assessment of ovarian follicular and luteal vascular flow using colour doppler ultrasonography. | our goal was to develop an objective computer-assisted volumetric method of assessing vascular flow from colour doppler ultrasound data of ovarian structures recorded by free-hand movement. we hypothesized that a vascularity index (ratio of the region of blood flood to the region of ovarian structure) obtained from the three-dimensional volumetric analysis would be more precise (less variable) than conventional two-dimensional analysis of single images in estimating the functional status of the ... | 2019 | 31302433 |
| serum haptoglobin and protein electrophoretic fraction modifications in buffaloes (bubalus bubalis) around calving and during early lactation. | serum protein distribution and concentration can be affected by different physiological and pathological conditions. the aim of this study was to evaluate the changes in the concentration of serum protein fractions and haptoglobin in clinically healthy dairy buffaloes during late pregnancy and early lactation. blood and milk samples were collected from 30 buffaloes at around 7 d before expected calving (blood only) and 7, 30 and 50 d after calving. in serum samples, the total protein, haptoglobi ... | 2019 | 31292012 |
| alpha lipoic acid supplementation ameliorates the wrath of simulated tropical heat and humidity stress in male murrah buffaloes. | a supplement which ameliorates temperature-humidity menace in food producing livestock is a prerequisite to develop climate smart agricultural packages. a study was conducted to investigate the heat stress ameliorative efficacy of alpha lipoic acid (ala) in male murrah water buffaloes (bubalus bubalis). eighteen animals (293.61 ± 4.66kg bwt) were randomly allocated into three groups (n = 6); nhsc (non-heat-stressed control), hs (heat-stressed) and hsla (heat-stressed-supplemented with ala@32 mg/ ... | 2019 | 31280374 |
| characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (bubalus bubalis) in early gestation. | placenta is formed by a parenchyma rich in extracellular matrix (ecm), and this structure guarantees the proper development of the embryo and placental functioning. recently, studies have focused on the characterization of ecm in the placenta and foetal membranes of different species. this work aimed to analyse the composition of the ecm and to quantify the types of collagens in its composition. for this, 33 chorioallantoic membranes were used at different gestational ages, which were grouped in ... | 2019 | 31278783 |
| characterization of circular rnas in chinese buffalo (bubalusbubalis) adipose tissue: a focus on circular rnas involved in fat deposition. | circular rnas (circrnas) have been identified as a novel type of regulators involved in multiple biological processes. however, circrnas with a potential function in fat deposition in buffalo are poorly understood. in this study, six rna libraries of adipose tissue were constructed for three young and three adult chinese buffaloes with paired-ends rna sequencing using the illumina hiseq 3000 platform. a total of 5141 circrnas were computationally identified. among them, 252 circrnas were differe ... | 2019 | 31266200 |
| reduced cytochrome oxidase activity and increased protein tyrosine phosphorylation of mitochondria-rich fractions of buffalo (bubalus bubalis) spermatozoa after a cycle of freezing and thawing. | the motility and fertility of mammalian spermatozoa are compromised when they are cryopreserved. sperm mitochondrial proteins play a vital role in conferring motility. however, the effects of cryopreservation on mitochondria-specific proteins remain primarily unexplored in domestic animals, including buffaloes, so the present study aimed to evaluate this issue. mitochondria were isolated from both non-cryopreserved and cryopreserved buffalo spermatozoa by sonication followed by sucrose density g ... | 2019 | 31233700 |
| in vitro embryo production in buffalo species (bubalus bubalis). | in the last years there has been a growing attention in the world regarding buffalo (bubalus bubalis) breeding. in tropical countries, climatic and environmental conditions make buffalo an irreplaceable milk producer. since cattle cannot express their own productive potential in these conditions, buffaloes are best suited to satisfy animal protein demand, as they are easy to maintain, long-lived, and parasite resistant. here we describe how to produce buffalo embryos from slaughterhouse oocytes, ... | 2019 | 31230281 |
| treatment of buffalo (bubalus bubalis) somatic cell nuclear transfer embryos with microrna-29b mimic improves their quality, reduces dna methylation, and changes gene expression without affecting their developmental competence. | microrna-29b (mir-29b) plays an important role in controlling dna methylation in cells. we investigated its role during early embryonic development in buffalo embryos produced by somatic cell nuclear transfer (scnt) and in vitro fertilization (ivf). mir-29b expression was highest at the 2-cell stage, decreased (p < 0.001) at the 4-cell stage, and remained low thereafter at the 8-cell, morula, and blastocyst stages, showing a similar pattern in cloned and ivf embryos. treatment of reconstructed e ... | 2019 | 31199675 |