| epidemiology of trichinellosis in germany, 1996-2006. | the epidemiology of trichinellosis in germany was investigated by analyzing national surveillance data from 1996-2006. during this period, 95 laboratory-confirmed infections and 12 outbreaks were reported. the highest mean annual incidence was found in immigrants from southeast european countries (0.3 cases/100,000 vs. 0.01 cases/100,000 in the german population) with an incidence rate ratio of 26.0 (95% confidence interval 11.6-51.8). the predominant clinical symptom was muscle pain in 79 patie ... | 2008 | 18171103 |
| effect of liquid swine manure rate, incorporation, and timing of rainfall on phosphorus loss with surface runoff. | excessive manure phosphorus (p) application increases risk of p loss from fields. this study assessed total runoff p (tpr), bioavailable p (bap), and dissolved reactive p (drp) concentrations and loads in surface runoff after liquid swine (sus scrofa domesticus) manure application with or without incorporation into soil and different timing of rainfall. four replicated manure p treatments were applied in 2002 and in 2003 to two iowa soils testing low in p managed with corn (zea mays l.)-soybean ... | 2008 | 18178885 |
| bone regeneration in osseous defects-application of particulated human and bovine materials. | different bone substitute materials are used to manage the challenge of local bone loss subsequent to craniofacial reconstructive surgery. in this animal study we examined the de novo bone formation in bone defects after insertion of puros allograft of human origin or navigraft of bovine origin, and compared the regenerative potential of each material to that of autogenous bone. | 2008 | 18206408 |
| a subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels. | hyperpolarization-activated cyclic nucleotide-gated (hcn) channels contribute to stabilizing resting membrane potential, thus controlling neuron excitability. subclasses of nociceptive neurons differ in their excitability, therefore, these channels could be a distinguishing marker. we investigated isolated dorsal root ganglion neurons from a non-rodent species, the pig, sus scrofa domesticus. single labeling revealed capsaicin-induced cobalt-uptake in 54.3% and transient receptor potential v1 (t ... | 2008 | 18218331 |
| genetic imprinting of h19 and igf2 in domestic pigs (sus scrofa). | the genes insulin-like growth factor 2 (igf2) and h19 express paternally and maternally, respectively, in humans, mice, sheep, and cattle. additionally, igf2 has been shown to be regulated by at least four promoters in a tissue- or development-specific manner. in the domestic pigs, the promoter- and tissue-specific imprinting pattern of igf2 has not been well characterized, nor is the imprinting pattern of h19. in the present study, we identified two polymorphisms in each of igf2 (exons 2 and 9) ... | 2008 | 18228173 |
| snipork - a microarray of snps in candidate genes potentially associated with pork yield and quality - development and validation in commercial breeds. | snipork is an oligonucleotide microarray based on the arrayed primer extension (apex) technique, allowing genotyping of single nucleotide polymorphisms (snps) in genes of interest for pork yield and quality traits. apex consists of a sequencing reaction primed by an oligonucleotide anchored with its 5' end to a glass slide and terminating one nucleotide before the polymorphic site. extension with one fluorescently labeled dideoxynucleotide complementary to the template reveals the polymorphism. ... | 2008 | 18228175 |
| prostasome-like vesicles stimulate acrosome reaction of pig spermatozoa. | the presence of small membranous particles characterizes the male genital fluids of different mammalian species. the influence of semen vesicles, denominated prostasomes, on sperm functional properties has been well documented in humans, but their biological activity is scarcely known in other species. the present work investigated prostasome-like vesicles in pig semen for their ability to interact with spermatozoa and to affect acrosome reaction. | 2008 | 18234073 |
| the contribution of social effects to heritable variation in finishing traits of domestic pigs (sus scrofa). | social interactions among individuals are ubiquitous both in animals and in plants, and in natural as well as domestic populations. these interactions affect both the direction and the magnitude of responses to selection and are a key factor in evolutionary success of species and in the design of breeding schemes in agriculture. at present, however, very little is known of the contribution of social effects to heritable variance in trait values. here we present estimates of the direct and social ... | 2008 | 18245326 |
| selection in the making: a worldwide survey of haplotypic diversity around a causative mutation in porcine igf2. | domestic species allow us to study dramatic evolutionary changes at an accelerated rate due to the effectiveness of modern breeding techniques and the availability of breeds that have undergone distinct selection pressures. we present a worldwide survey of haplotype variability around a known causative mutation in porcine gene igf2, which increases lean content. we genotyped 34 snps spanning 27 kb in 237 domestic pigs and 162 wild boars. although the selective process had wiped out variability f ... | 2008 | 18245828 |
| setup and dosimetry for exposing anaesthetised pigs in vivo to 900 mhz gsm mobile phone fields. | the aim of this study was a dosimetrical analysis of the setup used in the exposure of the heads of domestic pigs to gsm-modulated radio frequency electromagnetic fields (rf-emf) at 900 mhz. the heads of pigs were irradiated with a half wave dipole using three different exposure routines; short bursts of 1-3 s at two different exposure levels and a continuous 10-min exposure. the electroencephalogram (eeg) was registered continuously during the exposures to search for rf-emf originated changes. ... | 2008 | 18247366 |
| a comparative study on the effects of tylosin on select bacteria during continuous flow culture of mixed populations of gut microflora derived from a feral and a domestic pig. | continuous flow cultures of feral (culture fc) and domesticated (culture rpcf) pig gut microflora were established in steady state. cultures were continuously infused with 25 or 100 microg tylosin/ml and sampled at intervals to assess effects on total culturable anaerobes, bacteroides and enterococcus via plating to agar supplemented without or with 100 microg tylosin/ml, the latter to assess bacterial sensitivity to tylosin. endogenous tylosin-insensitive anaerobes within the cultures, while si ... | 2008 | 18260812 |
| pharmacological analyses of two naturally occurring porcine melanocortin-4 receptor mutations in domestic pigs. | the melanocortin-4 receptor (mc4r) is critical in regulating mammalian food intake and energy expenditure. numerous mutations in the mc4r gene have been identified from obese humans. so far two naturally occurring porcine mc4r (pmc4r) mutations, d298n and r236h, have been identified from various strains of pigs and d298n is being utilized as a genetic marker to screen performance traits of pigs. in this study, we performed functional analyses of pmc4r d298n and r236h, including their ligand bind ... | 2008 | 18262384 |
| development of a transoral fundoplication device and related experimental research. | this paper describes a device and surgical techniques developed between 1999 and 2003 to enable an entirely transoral approach to fundoplication. the endofundoplication system (efs) system consisted of a multifunctional flexible tube for oral introduction (18 mm) as the key component, with a specially designed retroverted grasper that was used to grasp the lower esophageal sphincter (les) area of the esophagus, for invaginating the les into the stomach and folding the gastric wall onto the wall ... | 2008 | 18270877 |
| cerebral cortical microvascular flow during and following cardiopulmonary resuscitation after short duration of cardiac arrest. | to examine changes in cerebral cortical macro- and microcirculation and their relationship to the severity of brain ischaemia during and following resuscitation from a short duration of cardiac arrest. | 2008 | 18280632 |
| concepts for visualization of multidirectional phase-contrast mri of the heart and large thoracic vessels. | multidirectional phase-contrast magnetic resonance imaging allows the acquisition of time-resolved velocity fields (vectors) of cardiac and vascular blood flow. its unique ability to provide vectorial flow information promises to give new insights into hemodynamic physiology. however, up to now appropriate and standardized procedures and software tools are missing to take advantage of all the information contained in the data. the objective of this work is to present a new versatile software too ... | 2008 | 18280934 |
| caspase-3 activation and dna damage in pig skin organ culture after solar irradiation. | in the present study, a convenient and easy-to-handle skin organ culture was developed from domestic pig ears using polycarbonate transwell culture inserts in 12-well plate. this alternative model was then tested for its suitability in analyzing the short-term effects of a single solar radiation dose (from 55 to 275 kj.m(-2)). differentiation of the pig skin was maintained for up to 48 h in culture, and its morphology was similar to that of fresh human skin. solar irradiation induced a significa ... | 2008 | 18282184 |
| tissue response to surgical energy devices. | there is little data available on the effects of energy-based surgical devices (esd) on tissues other than arteries and veins. as such, we quantified the lateral thermal damage associated with contemporary esd: the harmonic ace, a prototype bipolar device known as the gyrus trisector, the harmonic lcs-c5, and the ligasure v. | 2008 | 18289646 |
| microwave ablation with triaxial antennas tuned for lung: results in an in vivo porcine model. | to prospectively determine in swine the size and shape of coagulation zones created in normal lung tissue by using small-diameter triaxial microwave antennas and to prospectively quantify the effects of bronchial occlusion and multiple antennas on the coagulation zone. | 2008 | 18292471 |
| indirect estimation of porcine parvovirus maternal immunity decay in free-living wild boar (sus scrofa) piglets by capture-recapture data. | linear mixed regression and logspline density estimation were performed to estimate the survival curve and half life of passively acquired antibodies against porcine parvovirus (ppv) in 44 wild boar piglets captured in the northern apennines, italy. one piglet had no detectable maternal antibodies at 2.5 months post partum and no antibodies were detected in any of the remaining piglets by 4 months of age. fitted survival curves indicated that maternal antibodies were undetectable from 2.5 to 6 m ... | 2009 | 18295517 |
| production of transgenic and non-transgenic clones in miniature pigs by somatic cell nuclear transfer. | miniature pigs have been recognized as valuable experimental animals in various fields such as medical and pharmaceutical research. however, the amount of information on somatic cell cloning in miniature pigs, as well as genetically modified miniature pigs, is much less than that available for common domestic pigs. the objective of the present study was to establish an efficient technique of cloning miniature pigs by somatic cell nuclear transfer. a high pregnancy rate was achieved following tra ... | 2008 | 18296867 |
| hepatitis e virus: molecular virology, clinical features, diagnosis, transmission, epidemiology, and prevention. | hepatitis e virus (hev), the sole member of the genus hepevirus in the family of hepeviridae, is the major cause of several outbreaks of waterborne hepatitis in tropical and subtropical countries and of sporadic cases of viral hepatitis in endemic and industrialized countries. transmission of hev occurs predominantly by the fecal-oral route although parenteral and perinatal routes have been implicated. the overall death rate among young adults and pregnant women is 0.5-3% and 15-20%, respectivel ... | 2008 | 18297720 |
| porcine circovirus type 2 (pcv2) viral components immunomodulate recall antigen responses. | porcine circovirus type 2 (pcv2) is a single-stranded circular dna virus infecting domestic pigs worldwide. interaction of this virus with the immune system apparently modulates the immune response of the host. in the present study, the implication of different components of pcv2 in the modulation of the immune response of the host were investigated by using pcv2 viral-like particles (vlps) and 16 novel oligodeoxyribonucleotides containing cpg motifs (cpg-odns) based on the pcv2 genomic sequence ... | 2008 | 18304652 |
| comparison of a novel one-step percutaneous nephrolithotomy sheath with a standard two-step device. | percutaneous nephrolithotomy (pcnl) is the treatment of choice for large renal calculi. the most widely used access sheath for pcnl involves two steps including placement of a high-pressure balloon catheter (hpbc) followed by advancement of a sheath over the balloon. a novel pcnl balloon/sheath combination (nss) has been developed that allows for both dilation and sheath placement in a single step. the objective of this study was to compare the safety and efficacy of the nss to the hpbc in a por ... | 2008 | 18308088 |
| bioavailability of heme iron in biscuit filling using piglets as an animal model for humans. | the objective of this work was to evaluate the bioavailability of heme iron added to biscuit filling. it comprised two stages: first, the development of the heme iron enriched biscuit filling; second, the evaluation of the bioavailability of the mineral in fattening piglets. two groups were selected randomly and fed: a) low iron feed and biscuits with heme iron supplemented filling; b) normal feed (with ferrous sulphate). weight and blood parameters were measured every fifteen days. averages wer ... | 2008 | 18311330 |
| potential for cavitation-mediated tissue damage in shockwave lithotripsy. | shockwave lithotripsy (swl) injures renal tissue, and cavitation has been reported to mediate some of these effects. much of the work characterizing the cavitation injury of swl has been performed in small animals or in vitro. we describe experiments that promote cavitation during swl and estimate the spatial distribution of the resulting hemorrhagic lesion in a large-animal (porcine) model of clinical lithotripsy. | 2008 | 18315482 |
| thermal effects of laparoscopic saline-enhanced radiofrequency cautery on renal parenchyma in a porcine model. | advances in the field of surgery have recently developed a number of devices and materials to improve surgical hemostasis. the effects of one such device, saline-cooled monopolar radiofrequency electrocautery, has been well scrutinized in splenic and hepatic applications. despite the utilization of this device in urologic surgery, the classification of the thermal effects has been poorly characterized to date. the purpose of this study was to discern the thermal characteristics of the device in ... | 2008 | 18315485 |
| simulating the spread of classical swine fever virus between a hypothetical wild-boar population and domestic pig herds in denmark. | denmark has no free-range wild-boar population. however, danish wildlife organizations have suggested that wild boar should be reintroduced into the wild to broaden national biodiversity. danish pig farmers fear that this would lead to a higher risk of introduction of classical swine fever virus (csfv), which could have enormous consequences in terms of loss of pork exports. we conducted a risk assessment to address the additional risk of introducing and spreading csfv due to the reintroduction ... | 2008 | 18339438 |
| similarity of the swine vasculature to the human carotid bifurcation: analysis of arterial diameters. | we assessed the similarities of the iliac bifurcation in two breeds of swine to the known human carotid bifurcation, in order to assess its applicability as a preclinical model of the human carotid bifurcation. | 2008 | 18341957 |
| production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries. | for production of viable somatic cell nuclear transferred (scnt) miniature pig embryos, in vitro condition for controlling the quality of recipient oocytes derived from domestic pig ovaries should be evaluated. in the present study, to get information on optimal in vitro maturation (ivm) condition of oocytes, we investigated the effect of ivm duration of recipient oocytes on subsequent development of scnt miniature pig embryos, the maturation-promoting factor (mpf) activity in recipient oocytes ... | 2008 | 18352818 |
| rapid communication: robot-assisted notes nephrectomy: initial report. | natural orifice transluminal endoscopic surgery (notes) using the davinci robot (intuitive surgical, sunnyvale, ca) has never been applied to urologic surgery. here we present our initial experience with a combined transvaginal and transcolonic, single-port, robot-assisted notes nephrectomy. | 2008 | 18355143 |
| comparison of a novel radially dilating balloon ureteral access sheath to a conventional sheath in the porcine model. | traditional ureteral access sheaths rely on tapered dilators and the dotter principle of axial force to gain access into the ureter. we compared the performance of a novel balloon expandable ureteral access sheath using radial dilatation with that of a conventional ureteral access sheath. | 2008 | 18355865 |
| myocardial cytokine il-8 and nitric oxide synthase activity during and after resuscitation: preliminary observations in regards to post-resuscitation myocardial dysfunction. | increases in serum cytokines have been reported after successful resuscitation from prolonged ventricular fibrillation (vf). pro-inflammatory cytokines can stimulate inducible nitric oxide synthase (inos) to produce excessive levels of nitric oxide (no). high levels of both myocardial inflammatory cytokines and nitric oxide levels can depress myocardial contractile function. we hypothesized that myocardial pro-inflammatory cytokines and inos activity would increase following successful resuscita ... | 2008 | 18359140 |
| expec-typical virulence-associated genes correlate with successful colonization by intestinal e. coli in a small piglet group. | upon studying the transmission of escherichia coli from a sow to five of her piglets, we observed domination of the coliform flora in piglets by a single e. coli clone, especially after weaning. this haemolytic cloneh1 did not harbour any virulence determinants typical for intestinal pathogenic e. coli isolates from swine but had a virulence gene profile very similar to extraintestinal e. coli (expec), including genes coding for p fimbriae and several iron acquisition systems, besides having an ... | 2008 | 18373678 |
| cytokine mrna expression and pathological findings in pigs inoculated with african swine fever virus (e-70) deleted on a238l. | african swine fever virus (asfv) induces a variety of immune responses and clinical forms in domestic pigs. as it is the only member of the asfarviridae family, asfv encodes many novel genes not encoded by other virus families. among these genes, a238l may regulate the synthesis of pro-inflammatory cytokines, controlled mainly by nfkappab and nfat pathways. in this study, we inoculated two groups of pigs, one with the asfv highly virulent e-70 isolate, deleted on a238l gene, and the other group ... | 2008 | 18384883 |
| development of the movement of the epiglottis in infant and juvenile pigs. | although backward folding of the epiglottis is one of the signal events of the mammalian adult swallow, the epiglottis does not fold during the infant swallow. how this functional change occurs is unknown, but we hypothesize that a change in swallow mechanism occurs with maturation, prior to weaning. using videofluoroscopy, we found three characteristic patterns of swallowing movement at different ages in the pig: an infant swallow, a transitional swallow and a post-weaning (juvenile or adult) s ... | 2008 | 18387794 |
| detection and analysis of porcine circovirus type 1 in hungarian wild boars: short communication. | porcine circovirus type 1 (pcv1) is considered to be a non-pathogenic virus detected in cell cultures, vaccines or products used for cell culture preparations, all of them of porcine origin. serological evidence and genetic studies suggested that pcv1 was widespread in domestic pigs. the presence of pcv1 in wild boars in germany was also described using serological methods. this paper reports the first detection of pcv1 in hungarian wild boars. samples were collected at slaughterhouses and proce ... | 2008 | 18401965 |
| chitosan hemostatic dressing for renal parenchymal wound sealing in a porcine model: implications for laparoscopic partial nephrectomy technique. | this study was to evaluate the feasibility of using a novel chitosan hemostatic dressing to control hemorrhage and urinary leakage by sealing off the parenchymal wound following lpn. | 2008 | 18402734 |
| plasmin-matrix metalloproteinase cascades in spinal response to an experimental disc lesion in pig. | proteinases were immunohistochemically stained to analyze degenerated discs and paradiscal tissues in comparison to contiguous control tissues in an experimental porcine model of intervertebral disc degeneration. | 2008 | 18404101 |
| conventional high pressure versus newly developed continuous-flow ureterorenoscope: urodynamic pressure evaluation of the renal pelvis and flow capacity. | we evaluated the pressure and flow relation of a newly developed continuous-flow ureterorenoscope (urs) in comparison with a common ureterorenoscope in an ex-vivo urinary tract model. | 2008 | 18419336 |
| bone regeneration after topical bmp-2-gene delivery in circumferential peri-implant bone defects. | the aims of this study were to evaluate the rate of bone formation and osseointegration after topical gene delivery with a liposomal vector system carrying bone morphogenetic protein (bmp)-2 cdna in combination with a collagen carrier and autologous bone as a carrier in freshly created peri-implant bone defects. | 2008 | 18422983 |
| neurocognitive performance after cardiopulmonary resuscitation in pigs. | to test the feasibility of a neurocognitive test based on operant conditioning in a porcine model of cardiac arrest and cardiopulmonary resuscitation. furthermore, to characterize the influence of different durations of cardiac arrest on cognitive performance and the accompanying neurohistopathological changes. | 2008 | 18431271 |
| prearrest administration of low-molecular-weight heparin in porcine cardiac arrest: hemodynamic effects and resuscitability. | both animal and human studies demonstrate activation of coagulation during cardiac arrest. prearrest anticoagulation is used routinely in many experimental studies. we studied the hemodynamic effects of prearrest anticoagulation with a low-molecular-weight heparin suitable for clinical use during cardiopulmonary resuscitation in pigs. | 2008 | 18431276 |
| influenza exposure in united states feral swine populations. | swine play an important role in the disease ecology of influenza. having cellular receptors in common with birds and humans, swine provide opportunities for mixed infections and potential for genetic reassortment between avian, human, and porcine influenza. feral swine populations are rapidly expanding in both numbers and range and are increasingly coming into contact with waterfowl, humans, and agricultural operations. in this study, over 875 feral swine were sampled from six states across the ... | 2008 | 18436668 |
| hybrid natural orifice translumenal surgery (notes) sleeve gastrectomy: a feasibility study using an animal model. | the indications for natural orifice translumenal surgery (notes) are yet to be determined. morbidly obese patients may be one population that would benefit from this approach due to the elimination of wound complications and possibly a faster recovery. as a bariatric restrictive procedure, sleeve gastrectomy could be one indication for notes. to test the feasibility of this procedure with a notes approach, a pig model was used. | 2008 | 18437477 |
| gene silencing of cd47 and antibody ligation of thrombospondin-1 enhance ischemic tissue survival in a porcine model: implications for human disease. | insufficient tissue perfusion underlies many acute and chronic diseases. tissue perfusion in turn requires adequate blood flow, determined in large part by the relative state of relaxation or constriction of arterial vessels. nitric oxide (no) produced by vascular cells modulates blood flow and tissue perfusion by relaxing and dilating arteries. recently, we reported that the secreted protein thrombospondin-1 (tsp1), through its cell surface receptor cd47, limits the ability of no to relax and d ... | 2008 | 18438125 |
| epidemiological survey of swine influenza a virus in selected wild boar populations in germany. | the aim of this study was to evaluate the epidemiological situation of swine influenza virus (siv) infections in different wild boar populations in germany based on a serological surveillance in some bundeslaender (federal states) in connection with virological investigations in wild boar shot in northern germany (mecklenburg-western pomerania, district of nordvorpommern). altogether, 1245 sera from wild boar were tested using the hemagglutination inhibition test. the established seroprevalence ... | 2008 | 18440732 |
| transcription analysis in the melim swine model identifies rack1 as a potential marker of malignancy for human melanocytic proliferation. | metastatic melanoma is a severe disease. few experimental animal models of metastatic melanoma exist. melim minipigs exhibit spontaneous melanoma. cutaneous and metastatic lesions are histologically similar to human's. however, most of them eventually spontaneously regress. our purpose was to investigate whether the melim model could reveal markers of malignancy in human melanocytic proliferations. | 2008 | 18442364 |
| imaging of the coronary venous system: validation of three-dimensional rotational venous angiography against dual-source computed tomography. | information on the anatomy of the cardiac venous system (cvs) is increasingly important for cardiac resynchronization therapy or percutaneous transvenous mitral valve annuloplasty. three-dimensional (3d) imaging can further improve the understanding of the relationship of cardiac structures. this study was performed to validate the accuracy of rotational coronary sinus angiography (csa) displaying the 3d anatomy of the cvs compared to ecg-gated, contrast-enhanced, cardiac dual-source computed to ... | 2008 | 18449597 |
| portal vein segmentation of a 3d-planning system for liver surgery--in vivo evaluation in a porcine model. | computer systems allow the planning of complex liver operations. the segmentation of intrahepatic vessels builds the basis for the calculation of liver segments and resection proposals. for surgical use, it is essential to know the capabilities and limitations of the segmentation. the aim of this study was to determine the sensitivity and precision of the portal vein segmentation of a computer planning system for liver surgery in vivo. | 2008 | 18449610 |
| influence of methylmalonyl-coa mutase alleles on resistance to bovine tuberculosis in the european wild boar (sus scrofa). | an association study was carried out to examine the influence of methylmalonyl-coa mutase (mut) polymorphisms on the susceptibility of a well-studied wild boar population from southern spain to develop bovine tuberculosis (btb). to this end, we examined polymorphisms at a closely linked dinucleotide microsatellite flanking exon 2 of the mut gene in 37 wild boars with btb and 36 non-infected individuals. the microsatellite showed low polymorphism in the studied population, with only three alleles ... | 2008 | 18454807 |
| robotic notes (natural orifice translumenal endoscopic surgery) in reconstructive urology: initial laboratory experience. | to present an initial experience with robotic natural orifice translumenal surgery (r-notes) in reconstructive urology using the da vinci surgical system. | 2008 | 18455769 |
| low levels of haptoglobin and putative amino acid sequence in taiwanese lanyu miniature pigs. | porcine haptoglobin (hp) is an acute phase protein. its plasma level increases significantly during inflammation and infection. one of the main functions of hp is to bind free hemoglobin (hb) and inhibit its oxidative activity. in the present report, we studied the hp phenotype of taiwanese lanyu miniature pigs (tly minipigs; n=43) and found their hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human hp 1-1. interestingly, western blot and high performance liquid chromatographi ... | 2008 | 18460833 |
| foreign-body granulomas within intramyocardial arteries in a transcoronary safety assessment in pigs. | foreign-body granulomas within intramyocardial arteries were detected in three domestic pigs (sus scrofa domestica) in a routine transcoronary safety assessment study. the foreign bodies stained light grayish-blue by hematoxylin and eosin (h&e) and were strongly birefringent by polarized light microscopy. by their morphological features, they were identified as cotton fibers. embolization of foreign-body material into the myocardial arteries following catheter-based procedures is an unusual even ... | 2008 | 18467686 |
| transumbilical endoscopic cholecystectomy with the trichannel trocar technique: a porcine feasibility study. | the aim of this study is to verify the feasibility of another option: transumbilical endoscopic cholecystectomy by using the trichannel trocar technique. eight domestic pigs were submitted to transumbilical endoscopic cholecystectomy by using the trichannel trocar technique. a fine grasper of 2 mm in diameter was inserted through a small skin incision on the right upper abdomen for grasping the gallbladders. the natural scar of the umbilicus was restored after extract of the specimen. all the ga ... | 2008 | 18480087 |
| attainment of local drug delivery with paclitaxel-eluting balloon in porcine coronary arteries. | our purpose was to confirm the local drug delivery of a paclitaxel-eluting balloon by percutaneous intervention of single arterial segments or bifurcations of porcine coronary arteries. | 2008 | 18480668 |
| immediate post-shock chest compressions improve outcome from prolonged ventricular fibrillation. | this study was designed to test the hypothesis that immediate post-shock chest compressions improve outcome from prolonged ventricular fibrillation (vf) compared with typical "hands off" period (i.e., delayed post-shock compressions) associated with aed use. | 2008 | 18482786 |
| risk factors associated with renal parenchymal fracture during laparoscopic cryoablation. | to assess the risk factors for haemorrhage and renal fracture associated with renal cryoablation. | 2008 | 18485040 |
| prevalence of cryptosporidium genotypes in pre and post-weaned pigs in australia. | a total of 289 pig faecal samples were collected from pre-weaned and post-weaned piglets and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia and screened at the 18s rrna locus for the presence of cryptosporidium. an overall prevalence of 22.1% (64/289) was identified. cryptosporidium was more prevalent in post-weaned animals (p<0.05); 32.7% (51/156) versus 10.6% (13/123) for pre-weaned animals. sequence analysis identified cryptosporidium suis in ... | 2008 | 18486131 |
| attenuating the defibrillation dosage decreases postresuscitation myocardial dysfunction in a swine model of pediatric ventricular fibrillation. | the optimal biphasic defibrillation dose for children is unknown. postresuscitation myocardial dysfunction is common and may be worsened by higher defibrillation doses. adult-dose automated external defibrillators are commonly available; pediatric doses can be delivered by attenuating the adult defibrillation dose through a pediatric pads/cable system. the objective was to investigate whether unattenuated (adult) dose biphasic defibrillation results in greater postresuscitation myocardial dysfun ... | 2008 | 18496405 |
| diagnostic specificity of a real-time rt-pcr in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection. | foot-and-mouth disease virus (fmdv) and classical swine fever virus (csfv) are highly contagious and can cause great economic losses when introduced into disease-free regions. accurate estimates of diagnostic specificity (sp) are important when considering the implementation of surveillance for these agents. the purpose of this study was to estimate diagnostic sp of a real-time reverse-transcriptase pcr assay developed for detection of fmdv in cattle and domestic swine and csfv in domestic swine ... | 2008 | 18499360 |
| isolation and characterisation of rhodococcus equi from submaxillary lymph nodes of wild boars (sus scrofa). | rhodococcus equi has been isolated from the submaxillary lymph nodes of domesticated pigs, but little is known about the presence of r. equi in wild boars. the aim of the study was the evaluation of the incidence of r. equi in wild boars and the characterisation of them. of 482 submaxillary lymph nodes of wild boars shot in 39 settlements throughout hungary, r. equi was isolated from 60 specimens, and plasmid types of 82 isolates were examined. the isolates were tested for the presence of 15-17- ... | 2008 | 18499361 |
| greenlight (532 nm) laser partial nephrectomy followed by suturing of collecting system without renal hilar clamping in porcine model. | to assess the feasibility of partial nephrectomy (pn) without renal hilar clamping using the 80-w greenlight (532 nm) laser with opening of the collecting system followed by its suture in a porcine model. | 2009 | 18502479 |
| non-robotic thoracoscopic internal mammary artery preparation in the pig. a training model. | notwithstanding non-robotic, thoracoscopic preparation of the internal mammary artery (ima) is a difficult surgical task, an appropriate experimental training model is lacking. we evaluated the young domestic pig for this purpose. four domestic female pigs (30-40 kg body weight) were used for this study. bilateral thoracoscopic preparation of the ima was carried out under continuous, pressure controlled co(2) insufflation. a 30 degrees rigid thoracoscope was inserted through a 10-mm port in the ... | 2008 | 18502784 |
| [therapeutic effect of vacuum-assisted closure technology on infected explosion wound of pig]. | to investigate the effect of vacuum-assisted closure (v. a. c.) technology in the treatment of infected wound of skin and soft tissue as a result of explosion injury in pig. | 2008 | 18512552 |
| sex-related variation in compact bone microstructure of the femoral diaphysis in juvenile rabbits. | while gross morphological changes in the skeleton between males and females are well know, differences between sexes in the histomorphology are less known. it is important to have knowledge on the bone structure of rabbits, as this is a widely used species in biomedical research. a study was performed to evaluate the association between sex and the compact bone morphology of the femoral diaphysis in juvenile rabbits. | 2008 | 18522730 |
| nutritional skewing of conceptus sex in sheep: effects of a maternal diet enriched in rumen-protected polyunsaturated fatty acids (pufa). | evolutionary theory suggests that in polygynous mammalian species females in better body condition should produce more sons than daughters. few controlled studies have however tested this hypothesis and controversy exists as to whether body condition score or maternal diet is in fact the determining factor of offspring sex. here, we examined whether maternal diet, specifically increased n-6 polyunsaturated fatty acid (pufa) intake, of ewes with a constant body condition score around the time of ... | 2008 | 18541015 |
| [an investigation of the protective effect of prostaglandin e1 liposome on acute lung injury in pig]. | to assess the protective effect of prostaglandin e1 liposome (lipo-pge1) on acute lung injury (ali) in a porcine model of ali. | 2008 | 18549714 |
| pharmacokinetics of artesunate in the domestic pig. | the aim was to study the pharmacokinetic profile of artesunate and its metabolite dihydroartemisinin (dha) in a pig model. | 2008 | 18559353 |
| long beneficial effects after lipo-prostaglandin e1 administration on the ischemic gastric tube in pigs. | lipo-prostaglandin e1 (pge1) is a new preparation of pge1 in which it is bound to lipids in order to slow pge1 release and delay its rate of metabolism. we investigated how long the beneficial effects of intravenous administration of lipo-pge1 on the ischemic gastric tube continue. the gastric tube was constructed using 15 domestic pigs under general anesthesia and saline, unmodified pge1 and lipo-pge1 were infused continuously at a rate of 0.05 microg/kg/min for 10 minutes. tissue blood flow (t ... | 2008 | 18564164 |
| post-resuscitation hemodynamics and relationship to the duration of ventricular fibrillation. | we have observed consistent hemodynamic patterns after restoration of spontaneous circulation (rosc) after ventricular fibrillation (vf) cardiac arrest. we sought to characterize the time-course of these patterns, and to determine whether these differed based on duration of the vf insult. | 2008 | 18573588 |
| dynamic chemical characteristics of soil solution after pig manure application: a column study. | when manures from intensive livestock operations are applied to agricultural or vegetable fields at a high rate, large amounts of salts and metals will be introduced into soils. using a column leaching experiment, this study assessed the leaching potential of the downward movement of cu and zn as well as some salt ions after an intensive farm pig manure at rates of 0%, 5% and 10% (w/w) were applied to the top 20 cm of two different textured soils (g soil -sandy loam soil; h soil-silty clay loam ... | 2008 | 18576224 |
| renal injury and the application of polysaccharide hemospheres: a laparoscopic experimental model. | microporous polysaccharide hemospheres (mph) are hemostatic beads engineered from purified plant starch. mph accelerates the natural clotting cascade by concentrating clotting factors and proteins on their surface while absorbing aqueous and low molecular weight components from blood. the purpose of this study was to determine the efficacy of mph in achieving hemostasis in the setting of laparoscopic renal injury. | 2008 | 18578666 |
| laparoscopic repair of inferior vena caval injury using a chitosan-based hemostatic dressing. | vena caval injury is a rare but serious complication of laparoscopic surgery, and often requires conversion to an open procedure. the current study investigated whether a vena caval injury could be repaired with a chitosan dressing laparoscopically. | 2009 | 18585679 |
| carbon dioxide contrast agent for ct arteriography: results in a porcine model. | to test the feasibility of carbon dioxide (co(2))-enhanced computed tomographic (ct)-arteriography in an animal model. | 2008 | 18589320 |
| development of real-time polymerase chain reaction assays for rapid detection and differentiation of wild-type pseudorabies and gene-deleted vaccine viruses. | the successful eradication of pseudorabies in u.s. domestic swine was accomplished through the use of glycoprotein e (ge) deleted modified live virus vaccines and an accompanying ge differential enzyme-linked immunosorbent assay (elisa). yet, pseudorabies virus (prv) was established in feral swine in the united states, becoming a potential reservoir of prv for infection of domestic swine and other native wildlife. a critical need for the current prv surveillance program in the united states is t ... | 2008 | 18599848 |
| acalculous and clostridial cholecystitis in a pig. | a 21-month-old domestic hanford pig (sus scrofa domestica) in a 1-year study for experimental myocardial infarction was euthanized at the end of the study. one week earlier, the animal had symptoms and elevated clinical chemistry results suggestive of hepatobiliary disease, which resolved after medical therapy. at necropsy, the gallbladder was markedly enlarged, discolored, and had a thickened wall. within the gallbladder, there was abundant friable green-brown material. a culture of the gallbla ... | 2008 | 18599865 |
| molecular identification and prevalence of isospora sp. in pigs in western australia using a pcr-rflp assay. | little is known about the prevalence of isospora in domestic pigs in western australia. a total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia. faecal samples were screened using a pcr-rflp assay based on the its-1 rdna locus. an overall prevalence of 10.4% (30/289) was identified. isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-wea ... | 2008 | 18601925 |
| detection and size differentiation of simulated tooth root defects using flat-panel volume computerized tomography (fpvct). | our aim was to evaluate the capacity of flat-panel volume computerized tomography (fpvct) to enable the observer to detect and differentiate 3 different sizes of simulated tooth root defects in radiographs. | 2009 | 18602316 |
| patterns of crystallin distribution in porcine eye lenses. | to measure the protein distribution patterns in single young porcine lenses. | 2008 | 18615203 |
| domestic pigs have low susceptibility to h5n1 highly pathogenic avian influenza viruses. | genetic reassortment of h5n1 highly pathogenic avian influenza viruses (hpai) with currently circulating human influenza a strains is one possibility that could lead to efficient human-to-human transmissibility. domestic pigs which are susceptible to infection with both human and avian influenza a viruses are one of the natural hosts where such reassortment events could occur. virological, histological and serological features of h5n1 virus infection in pigs were characterized in this study. two ... | 2008 | 18617994 |
| serological evidence for hepatitis e virus infection in laboratory monkeys and pigs in animal facilities in japan. | in laboratory animal facilities, monkeys and pigs are used for animal experiments, but the details of hepatitis e virus (hev) infection in these animals are unknown. the risk of infection from laboratory animals to humans has become a concern; therefore, much attention should be paid to the handling of these animals during their care and use, including surgical procedures performed on infected animals. in this connection, serum samples collected from 916 monkeys and 77 pigs kept in 23 animal fac ... | 2008 | 18633159 |
| the differences between venous air embolism and fat embolism in routine intraoperative monitoring methods, transesophageal echocardiography, and fatal volume in pigs. | venous air embolism (vae) or fat embolism (fe) may occur in similar clinical settings such as after multiple injuries or total hip replacement. we designed this study to observe the differences between vae and fe in routine intraoperative monitoring methods, transesophageal echocardiography (tee), and fatal volume in pigs. | 2008 | 18636024 |
| distinct subtypes of urinary bladder epithelial cells with inducible and non-inducible cytochrome p450 1a1. | cultured primary porcine urinary bladder epithelial cells (pubec) represent an adequate and easy to handle in vitro system for studies of urothelial toxicity. pubec maintain in vivo-like metabolic activities and physiological functions. they express inducible cytochrome p4501a isoenzymes, which are of particular relevance, since they contribute to activation of bladder carcinogens. a possible drawback of pubec is their isolation from common domestic pigs that do not represent an inbred strain. i ... | 2009 | 18636242 |
| expression of mitochondrial transcription factor a (tfam) during porcine gametogenesis and preimplantation embryo development. | mitochondrial transcription factor a (tfam) is responsible for stability, maintenance, and transcriptional control of mitochondrial dna (mtdna). we have studied the expression and distribution of tfam in the gametes and preimplantation embryos of the domestic pig (sus scrofa). we hypothesized that tfam is not present in the boar sperm mitochondria to reduce the possibility of paternal mtdna propagation in the progeny. in contrast, we anticipated that tfam gene is expressed in a developmental sta ... | 2008 | 18636550 |
| detection of trichinella spiralis, t. britovi and t. pseudospiralis in muscle tissue with real-time pcr. | infections caused by trichinella species occur throughout the world in many wild and domestic animals resulting in trichinellosis in men. in europe, domestic pigs are predominantly infected by three trichinella species: t. spiralis, t. britovi and t. pseudospiralis. present methods for detection of trichinella spp. (compressorium method, artificial digestion) do not always sufficiently recognize trichinella larvae and these techniques are labor-intensive, time consuming and do not differentiate ... | 2008 | 18639594 |
| the effect of pest control on the incidence of trichinosis in virovitica-podravina county, croatia. | the aim of the study was to assess the role of pest control as a preventive measure in the control of trichinosis. the investigation was designed as a retrospective cohort study in the virovitica-podravina county. the incidence of trichinosis was determined according to the presence or absence of exposure to protective pest control measures. differences in the rate of trichinosis infection were determined by calculating relative risk (rr), i.e., the ratio of absolute risk in the two groups. in t ... | 2008 | 18644676 |
| comparison of selected skin decontaminant products and regimens against vx in domestic swine. | an anesthetized domestic swine model was used to compare the efficacy and cross-contamination potential of selected skin decontaminant products and regimens against the chemical warfare agent, vx. animals topically exposed to 2x, 3x or 5x ld(50) vx showed typical signs of organophosphate nerve agent poisoning, including miosis, salivation, mastication, dysrhythmias, and respiratory distress prior to death. animals were exposed to 5x ld(50) vx and then decontaminated 45 min later with the reactiv ... | 2008 | 18650258 |
| guidelines for the identification and development of sampling methods and design of suitable protocols for monitoring of trichinella infection in indicator species. | summary: according to the european community regulation no. 2075/2005, a risk-based program should be implemented in those areas where wildlife and pig holdings applying for trichinella-free status coexist, or for region where the risk of trichinella in domestic swine is officially recognised as negligible. trichinella nematode species are primarily parasites of wildlife which can transmit the infection to pigs intended for human consumption, acting as reservoir of the parasite. since not all ma ... | 2008 | 18660570 |
| insect succession and decomposition patterns on shaded and sunlit carrion in saskatchewan in three different seasons. | a study was conducted on decomposition and insect succession in the prairie ecozone of saskatchewan in the year 2000. eighteen domestic pig carcasses (42-79 kg) were employed as human models for applications to future homicide investigations in this region. two major variables were considered including the effect of season and habitat (sun versus shade). research was conducted over 25 weeks, spanning three seasons: spring, summer and fall. ambient temperature, internal carcass temperature, fauni ... | 2008 | 18662603 |
| does selective intubation increase ablation zone size during pulmonary cryoablation? | to determine the effect of selective intubation on ablation zone size during pulmonary cryoablation. | 2008 | 18662888 |
| influence of ventilation strategies on survival in severe controlled hemorrhagic shock. | to investigate the effect of different ventilation settings on hemodynamic stability in severe controlled hemorrhagic shock. | 2008 | 18679111 |
| endoscopic ultrasound-guided ethanol injection in the pancreas in a porcine model: a preliminary study. | despite aggressive multimodal treatments, survival rates for patients with pancreatic cancer remain disappointing. local progression is problematic, and minimally invasive procedures allowing locoregional control are needed. in this study, we attempted endoscopic ultrasound (eus)-guided injection of ethanol into the pancreas. | 2008 | 18702683 |
| hosts and habitats of trichinella spiralis and trichinella britovi in europe. | trichinella spiralis and trichinella britovi are the two most common species of trichinella circulating in europe. based on data provided to the international trichinella reference centre over the past 20 years (data referring to 540 isolates of t. spiralis and 776 isolates of t. britovi), we describe the host species and habitat characteristics for these two pathogens in europe. a geographical information system was constructed using administrative boundaries, a corine land cover (clc) map, and ... | 2009 | 18708065 |
| [molecular identification of heparin from pigs by allele-specific pcr (as-pcr) and amplification refractory mutation system (arms)]. | the aim of this study is to develop a convenient and effective method for the identification of heparin from pigs (include sus scrofa domestica brisson and sus scrofa riukiuanus). based on sequences of d-loop region of pigs and the other animals, two pairs of highly specific primers were designed for distinguishing heparin of pigs from other animals. the primers were employed to amplify d-loop region of dna templates extracted from pig and seven other animal species that amounted to 49 samples. ... | 2008 | 18717344 |
| human dispersal of trichinella spiralis in domesticated pigs. | to investigate the human impact on the evolutionary ecology of animal pathogens, we compared genetic diversity of severe foodborne parasites contracted by eating infected pork or wild game. in particular, we characterized trichinella spp. from twenty-eight countries and four continents by genotyping nine microsatellite loci and sequencing one-fifth of the mitochondrial genome. all specimens of trichinella spiralis, a swine parasite that can infect many species of wildlife, were remarkably unifor ... | 2008 | 18718558 |
| notes: a review of the technical problems encountered and their solutions. | natural orifice translumenal endoscopic surgery (notes) is currently investigated and developed worldwide. in the past few years, multiple groups have confronted this challenge. many technical problems are encountered in this technique due to the currently available tools for this approach. some of the unique technical problems in notes include: blindly performed primary incisions; uncontrolled pneumoperitoneal pressure; no support for the endoscope in the abdominal cavity; inadequate vision; in ... | 2008 | 18721009 |
| evaluation of unmeshed and 1:1 meshed alloderm bolsters for stapled rectal anastomoses in a porcine model. | the major morbidity of colorectal anastomoses is leaks. the concept of staple-line reinforcement is a growing area of interest. in this study, we evaluated the feasibility and effect of utilizing alloderm to bolster end-to-end stapled rectal anastomoses in a porcine model. | 2008 | 18721018 |
| epicardial pulmonary vein isolation: a long-term histologic and imaging animal study comparing cryothermy versus radiofrequency. | simplifications of the cox-maze procedure to treat atrial fibrillation (af) include epicardial pulmonary vein (pv) isolation using various sources to create lines of conduction block. the creation of transmural lesions has been a concern. the heat-sink effect caused by blood flow has limited the effectiveness of unipolar radiofrequency and linear application of cryothermy, which should be abolished when clamp devices are used for pv isolation. the efficacy and safety of epicardial beating-heart ... | 2008 | 18721573 |
| [frequency of chromosomes carrying endogenous retroviruses in the populations of domestic pig and wild boar]. | statistical analysis of the frequency of chromosomes carrying three types (a, b, and c) of porcine endogenous retroviruses (perv) was based on molecular-genetic testing of populations of domestic pigs and wild boars. domestic pigs were shown to have higher frequency of perv and haplotypes containing two and three types of perv than wild boars. | 2008 | 18727389 |
| manganese-enhanced mri of acute cardiac ischemia and chronic infarction in pig hearts: kinetic analysis of enhancement development. | to assess infarction development in pig hearts, mn-enhanced and gd-enhanced mri were used. in domestic pigs (25-35 kg, n = 37), the first and second diagonal branches of the left anterior descending coronary artery were ligated to induce acute ischemia and infarction (ischemia+reperfusion) or chronic infarction of increasing duration (3- 28 days). ex vivo experiments were performed on hearts perfused in the langendorff mode with a 50:50 mixture of blood and krebs-henseleit buffer using a spin-ec ... | 2009 | 18756440 |