| oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells. | the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies. | 2009 | 19702954 |
| the effect of lactoferrin on oral bacterial attachment. | lactoferrin (lf), an iron-binding salivary glycoprotein, plays an important role in human innate defense against local mucosal infection. we hypothesized that lf interferes with initial oral bacterial attachment to surfaces by iron sequestration, so inhibiting subsequent biofilm formation. the objective was to investigate the effect of lf on the early stages of single-species and multi-species oral biofilm development. | 2009 | 19702956 |
| contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. | clinical/microbiological studies have consistently revealed the persistence of some bacteria after conventional root canal debridement. although this was originally attributed to the complexity of the root canal anatomy and the difficulty of delivering antibacterial agents effectively, it has emerged that the biofilm encasement of bacterial cells may confer a further mechanism of resistance. the purpose of this study was to investigate the relative disruption and bactericidal effects of root can ... | 2009 | 19720223 |
| maternal periodontal disease and soluble fms-like tyrosine kinase-1 expression. | this study was conducted to examine the relationship between maternal periodontal disease and plasma angiogenic factor expression of soluble fms-like tyrosine kinase (sflt)-1. | 2009 | 19722802 |
| fusobacterium nucleatum endocarditis mimicking polymyalgia rheumatica. | a case of fusobacterium nucleatum endocarditis in an 80-year-old man is reported. the patient presented with a headache and nonspecific musculoskeletal symptoms and was misdiagnosed as having polymyalgia rheumatica. the diagnosis of bacterial endocarditis was delayed because of an insidious presentation, typical in infections with low virulence micro-organisms. the musculoskeletal symptoms, unresponsive to protracted corticosteroids, completely resolved with intravenous ampicillin treatment. rhe ... | 2009 | 19738533 |
| mutualistic biofilm communities develop with porphyromonas gingivalis and initial, early, and late colonizers of enamel. | porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. we report here that p. gingivalis atcc 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. integration of p. gingivalis into multispecies communities was investigated by using t ... | 2009 | 19749049 |
| mobilization and prevalence of a fusobacterial plasmid. | fusobacterium nucleatum is a gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. genomic analyses of f. nucleatum indicate considerable genetic diversity and a prominent role for horizontal gene transfer in the evolution of the species. several plasmids isolated from f. nucleatum, including pfn1, harbor relaxase gene homologs that may function in plasmid mobilizatio ... | 2010 | 19761790 |
| an unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria. | pyogenic liver abscess (pla) formation is thought to originate from the transmission of infection via three major routes including the biliary tract, portal vein and hepatic artery. however, about 50% of pla cases are considered to be cryptogenic. here we report an unusual autopsy case of pla associated with periodontopathic bacterial infection. a 59-year-old female suddenly developed cardiopulmonary arrest and died. despite macroscopic and microscopic examinations, the infectious routes and sou ... | 2009 | 19762989 |
| kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe fusobacterium nucleatum. | cofactor-independent glutamate racemases (grs) that supply the d-glutamate required for biosynthesis of the peptidoglycan that encapsulates bacterial cells are attractive targets for the development of antibacterial drugs. recombinant gr from fusobacterium nucleatum (fngr), a gram-negative anaerobe involved in periodontal disease, was overproduced, purified, and characterized. unlike most other grs, fngr is a pseudosymmetric enzyme, catalyzing the racemization of glutamate enantiomers with simil ... | 2009 | 19772853 |
| differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by porphyromonas gingivalis. | periodontitis is a polymicrobial infection caused by selected gram-negative bacteria including porphyromonas gingivalis. host cell invasion by p. gingivalis has been proposed as a possible mechanism of pathogenesis in periodontitis. the aim of the present study was to assess the influence of periodontopathogens on p. gingivalis invasion of gingival epithelial cells in polymicrobial infection. p. gingivalis was tested for its ability to invade a human gingival epithelial cell line ca9-22 in co-in ... | 2009 | 19818393 |
| photodynamic therapy of persistent pockets in maintenance patients-a clinical study. | the aim of this study was to compare the short-term performance of a session of single photodynamic therapy (pdt) and of a conventional ultrasonic debridement (ust) in persistent pockets of maintenance patients. in a prospective, randomized, controlled, single-blind clinical study, patients with chronic periodontitis with at least two persistent pockets (>4 mm) were enrolled. they were treated either with ust (n = 29) or pdt (n = 25). clinical and microbiological examinations were performed at b ... | 2010 | 19823880 |
| microbial interactions influence inflammatory host cell responses. | the inflammatory response plays an important role in the tissue destruction associated with periodontitis. bacterial species can regulate the inflammatory responses of host cells, triggered by pathogens. it was hypothesized that, in the field of oral microbiology/immunology, such effects of bacterial interactions on inflammatory host cell responses might also be present. in this study, the effects of beneficial, commensal, and pathogenic species on aggregatibacter actinomycetemcomitans-induced i ... | 2009 | 19828891 |
| involvement of cytosolic phospholipase a2alpha in mmp-9 up-regulation. | matrix metalloproteinase-9 (mmp-9) is important in the pathogenesis of periodontitis. cytosolic phospholipase a(2)alpha (cpla(2)alpha) is involved in mmp-9 up-regulation in human monocytes. we tested the hypothesis that cpla(2)alpha also regulates mmp-9 induction by fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (pma) in gingival epithelial cells. while pma induced mmp-9 expression considerably, f. nucleatum did so moderately. this time-course study demonstrated that mmp-9 mrna u ... | 2009 | 19828892 |
| progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque. | chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ... | 2009 | 19832799 |
| characterization of bacterial flora in persistent apical periodontitis lesions. | microorganisms are able to survive and induce persistent infection in periapical tissues. the aim of this study was to investigate the composition of the microflora of persistent apical periodontitis lesions. | 2009 | 19832803 |
| autoaggregation response of fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. this species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. numerous studies of f. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. we tested coaggregation between f. nucleatum atcc strain 25586 and various species ... | 2009 | 19837836 |
| antibiotic resistance of periodontal pathogens obtained from frequent antibiotic users. | the occurrence of bacterial strains resistant to different antimicrobials is a growing problem in latin american countries. the aim was to measure the antimicrobial susceptibility of fusobacterium nucleatum, prevotella intermedia and prophyromona gingivalis, isolated from chronic periodontitis patients. twenty-five patients diagnosed with generalized- moderate or advanced- chronic periodontitis were consecutively recruited from patients attending the periodontal specialist program, javeriana uni ... | 2009 | 19839485 |
| hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria. | hydrogen sulfide is one of the predominant volatile sulfur compounds (vscs) produced by oral bacteria. this study developed and evaluated a system for detecting hydrogen sulfide production by oral bacteria. | 2009 | 19905954 |
| sonic extracts from a bacterium related to periapical disease activate gelatinase a and inactivate tissue inhibitor of metalloproteinases timp-1 and timp-2. | to examine the effects of sonicated bacterial extracts (sbes) from three related to periapical disease bacteria (porphyromonas gingivalis, p. endodontalis and f. nucleatum) on the activation of matrix metalloproteinase (mmp-2) and the inactivation of tissue inhibitors of metalloproteinase (timp-1 and timp-2). | 2009 | 19912382 |
| acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum. | acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls. | 2011 | 19926616 |
| methamphetamine cytotoxicity and effect on lps-stimulated il-1beta production by human monocytes. | methamphetamine (meth) abuse is associated with "meth mouth", characterized by rampant dental decay and destruction of periodontal bone and soft tissues. in periodontitis, monocyte/macrophages, stimulated by bacterial lipopolysaccharide (lps), produce interleukin-1beta (il-1beta), contributing to bone and soft tissue degradation. effects of meth on monocyte/macrophages and its role in periodontitis are unknown. the objective of this study was to determine meth cytotoxicity and effects on constit ... | 2010 | 19945523 |
| fusobacterium bacteremia: clinical significance and outcomes. | fusobacterium bacteremia is uncommon, accounting for approximately 0.9% of patients with bacteremia. the objectives of this study were to evaluate the incidence and clinical significance of blood cultures positive for fusobacterium spp., risk factors for mortality, and the impact of antimicrobial therapy on clinical outcomes. | 2009 | 19949758 |
| application of rpob and zinc protease gene for use in molecular discrimination of fusobacterium nucleatum subspecies. | fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, f. nucleatum subsp. fusiforme, f. nucleatum subsp. vincentii, and f. nucleatum subsp. animalis) based on several phenotypic characteristics and dna-dna hybridization patterns. however, the methods for detecting or discriminating the clinical isolates of f. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. the ... | 2010 | 19955278 |
| transmission of periodontopathic bacteria from natural teeth to implants. | purpose: prevention of peri-implantitis is essential for the success of implant rehabilitation. infection by periodontopathic bacteria is a major cause of peri-implantitis. the aim of the present study was to identify the source of peri-implant colonization by periodontopathic bacteria. materials and methods: twenty-one patients with implants were enrolled in the study. subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage s ... | 2009 | 20002682 |
| identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach. | this study identified the bacterial taxa enduring endodontic treatment procedures by using a combined 16s ribosomal rna-based reverse-transcriptase polymerase chain reaction (rt-pcr) and reverse-capture checkerboard hybridization approach. | 2010 | 20003934 |
| fusobacterium nucleatum envelope protein foma is immunogenic and binds to the salivary statherin-derived peptide. | we have previously shown that one of the minimal active regions of statherin, a human salivary protein, for binding to fusobacterium nucleatum is a yqpvpe amino acid sequence. in this study, we identified the foma protein of f. nucleatum, which is responsible for binding to the statherin-derived yqpvpe peptide. overlay analysis showed that a 40-kda protein of the f. nucleatum cell envelope (40-kda ce) specifically bound to the yqpvpe peptide. the equilibrium association constant between the affi ... | 2010 | 20008529 |
| bacterial adhesins to host components in periodontitis. | | 2010 | 20017793 |
| nod1 and nod2 mediate sensing of periodontal pathogens. | in bacterial infection, nucleotide-binding oligomerization domain (nod) 1 and nod2 induce innate immune responses by recognizing fragments of the bacterial component peptidoglycan (pgn). to determine the roles of these receptors in detection of periodontal pathogens, we stimulated human embryonic kidney cells expressing nod1 or nod2 with heat-killed porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, and fusobacterium nucleatum or their soluble pgns (spgns). all bacteria and their s ... | 2010 | 20040739 |
| microbiologic testing and outcomes of full-mouth scaling and root planing with or without amoxicillin/metronidazole in chronic periodontitis. | it has been suggested that use of systemic antibiotics should be limited to patients with specific microbiologic profiles. the main purpose of the present analysis was to study whether microbiologic testing before therapy was of value in predicting which patients would specifically benefit from adjunctive amoxicillin and metronidazole, given in the context of full-mouth scaling and root planing (srp) within 48 hours. | 2010 | 20059413 |
| in vitro killing activity of crevicular concentrations of tinidazole plus common oral antibiotics against high-density mixed inocula of periodontal pathogens in strict anaerobic conditions. | odontogenic infections are polymicrobial. this study explores the in vitro killing activity by concentrations similar to those found in crevicular fluid of tinidazole in combination with amoxicillin/clavulanic acid, clindamycin and levofloxacin against four groups of high-density mixed inocula of anaerobes (prevotella buccae, fusobacterium nucleatum, and veillonella spp.) and facultative (capnocytophaga spp. and streptococcus spp.) isolates of periodontal pathogens. | 2010 | 20059425 |
| [pelvic actinomycosis: a misleading picture]. | pelvic actinomycosis is a rare chronic suppurative granulomatous disease associated with an intrauterine contraceptive device. next pelvic organs can be affected but also more distant organs, to lead to different clinical situations. the diagnosis is therefore often difficult. we report here the case of a woman who presents a particular extensive pelvic actinomycosis diagnosed after surgical treatment with important morbidity. this case shows the interest of evoking the diagnosis in an evocative ... | 2010 | 20074892 |
| substrate-dependent proton antiport in neurotransmitter:sodium symporters. | neurotransmitter-sodium symporters (nss), targets for psychostimulants and therapeutic drugs, have a critical role in neurotransmission. whereas eukaryotic nss show chloride-dependent transport, bacterial nss feature cl(-)-independent substrate transport. recently we showed that mutation of an acidic residue near one of the sodium ion-binding sites in leut of aquifex aeolicus or tyt1 of fusobacterium nucleatum renders substrate binding and/or transport cl(-) dependent. we reasoned that the negat ... | 2010 | 20081826 |
| efficacy of a low-concentration chlorhexidine mouth rinse in non-compliant periodontitis patients attending a supportive periodontal care programme: a randomized clinical trial. | to assess the clinical and microbiological efficacy of a 0.05% chlorhexidine and 0.05% cetyl-pyridinium chloride mouth rinse in supportive periodontal care (spc) in patients with inadequate plaque control. | 2010 | 20088984 |
| use of a novel assay to evaluate enzymes that produce hydrogen sulfide in fusobacterium nucleatum. | to evaluate enzymes that produce hydrogen sulfide (h(2)s) in species of fusobacterium nucleatum, we developed an assay based on sds-polyacrylamide gel electrophoresis with renaturation followed by active staining. this assay provided precise insight into the enzymes that produce h(2)s in terms of their number and molecular weights. | 2010 | 20093145 |
| term stillbirth caused by oral fusobacterium nucleatum. | intrauterine infection is a recognized cause of adverse pregnancy outcome, but the source of infection is often undetermined. we report a case of stillbirth caused by fusobacterium nucleatum that originated in the mother's mouth. | 2010 | 20093874 |
| epithelial cell pro-inflammatory cytokine response differs across dental plaque bacterial species. | the dental plaque is comprised of numerous bacterial species, which may or may not be pathogenic. human gingival epithelial cells (hgecs) respond to perturbation by various bacteria of the dental plaque by production of different levels of inflammatory cytokines, which is a putative reflection of their virulence. the aim of the current study was to determine responses in terms of interleukin (il)-1beta, il-6, il-8 and il-10 secretion induced by porphyromonas gingivalis, aggregatibacter actinomyc ... | 2010 | 20096064 |
| the bactericidal effect of a genius nd:yag laser. | to evaluate the 'in vitro' bactericidal effect of the nd:yag laser (genius, mølsgaard dental, copenhagen, denmark) on six periodontal pathogens. | 2010 | 20096084 |
| effects of methyl gallate and gallic acid on the production of inflammatory mediators interleukin-6 and interleukin-8 by oral epithelial cells stimulated with fusobacterium nucleatum. | interactions between periodontal bacteria and human oral epithelial cells can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. this study examined the effects of methyl gallate (mg) and gallic acid (ga) on the production of inflammatory mediators, interleukin (il)-6 and il-8, by oral epithelial cells stimulated by f. nucleatum. in a real- ... | 2010 | 20127471 |
| the antimicrobial efficacy of commercial dentifrices. | this investigation compared the effects of a fluoride dentifrice and toothpastes formulated with antimicrobial ingredients (stannous fluoride and triclosan/copolymer) on oral micro-organisms, including those found in samples taken from the human oral cavity. microbiological techniques determined the minimum inhibitory concentrations (mics) of each dentifrice necessary to inhibit the growth of bacterial strains from the healthy oral cavity, as well as those found in dental caries, periodontal dis ... | 2010 | 20129893 |
| effects of a mouthwash with chlorine dioxide on oral malodor and salivary bacteria: a randomized placebo-controlled 7-day trial. | previous research has shown the oxidizing properties and microbiological efficacies of chlorine dioxide (clo2). its clinical efficacies on oral malodor have been evaluated and reported only in short duration trials, moreover, no clinical studies have investigated its microbiological efficacies on periodontal and malodorous bacteria. thus, the aim of this study was to assess the inhibitory effects of a mouthwash containing clo2 used for 7 days on morning oral malodor and on salivary periodontal a ... | 2010 | 20152022 |
| central role of the early colonizer veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel. | human dental biofilm communities comprise several species, which can interact cooperatively or competitively. bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of one-, two- and three-species communities growing on saliva as the sole nutritional source. we investigated single-species and multispecies colonization by using known initial, early ... | 2010 | 20154130 |
| the relationship between physiologic halitosis and periodontopathic bacteria of the tongue and gingival sulcus. | to determine the influence of oral status on halitosis, the relationship between halitosis and periodontopathic bacteria present in plaque on the tongue and the subgingival sulcus was examined in 62 periodontally healthy adults. halitosis indicators used were the organoleptic score; gas chromatography results [total volatile sulfur compounds (vscs) = h(2)s + ch(3)sh + (ch(3))(2)s]; halimeter values; and the results of three clinical tests, plaque control record (plcr), plaque index (pli), and to ... | 2010 | 20155507 |
| lemierre syndrome: a japanese patient returning from thailand. | after returning from thailand, a 23-year-old japanese man was admitted because of fever, sore throat, neck pain, and chest pain. contrasted-enhanced ct scanning of his neck revealed an absence of flow through the right internal jugular vein representing thrombosis, and moreover, an increase in wall thickness of the right internal jugular vein and enhancement of the surrounding tissue representing thrombophlebitis. lung abscesses were also identified by a chest ct scan. fusobacterium nucleatum wa ... | 2010 | 20177952 |
| vaccination targeting surface foma of fusobacterium nucleatum against bacterial co-aggregation: implication for treatment of periodontal infection and halitosis. | the mechanical therapy with multiple doses of antibiotics is one of modalities for treatment of periodontal diseases. however, treatments using multiple doses of antibiotics carry risks of generating resistant strains and misbalancing the resident body flora. we present an approach via immunization targeting an outer membrane protein foma of fusobacterium nucleatum (f. nucleatum), a central bridging organism in the architecture of oral biofilms. neutralization of foma considerably abrogated the ... | 2010 | 20189489 |
| quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction. | chronic periodontitis is a highly prevalent endogenous polymicrobial disease. to better understand the etiology of the disease a quantitative approach is mandatory and real-time pcr is the molecular technique currently preferred to achieve this purpose. taking into account that such a kind of study is still scarce, we aimed to evaluate the association between periodontal microbiota and chronic periodontitis. a total of 60 low-income age-matched female adults, 30 with chronic periodontitis and 30 ... | 2010 | 20193770 |
| oral biofilm architecture on natural teeth. | periodontitis and caries are infectious diseases of the oral cavity in which oral biofilms play a causative role. moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and biofilm resistance to antibiotics, due to their widespread presence and accessibility. despite descriptions of initial plaque formation on the tooth surface, studies on mature plaque and plaque structure below the gum are limited to landmark studies from the 1970s, without app ... | 2010 | 20195365 |
| [anaerobic gram-negative bacilli involved in the etiopathogeny of the abscesses of superficial fascial spaces of the face and neck]. | the aim of this study was to isolate and identify at species level the strains of anaerobic gram-negative bacilli (gnb) from pus samples collected in patients with abscesses of fascial spaces of the face and neck. microscopy of gram-stained smears and cultures were performed in each specimen. the strictly anaerobic gnb strains were identified using the conventional methods of diagnosis and the rapid id 32 a system. in addition, the other strains isolated in association with these bacteria were i ... | 2008 | 20201270 |
| interplay of protease-activated receptors and nod pattern recognition receptors in epithelial innate immune responses to bacteria. | protease-activated receptors (pars), nucleotide-binding oligomerization domain (nod) receptors and toll-like receptors (tlrs) play a role in innate immunity, but little is known about interaction between these receptors. the goal of this study was to investigate how silencing one receptor affects the expression of other receptors and downstream innate immune markers in response to bacteria. human gingival epithelial cells (gecs) were transfected with sirna specific for par1 or par2, then stimula ... | 2010 | 20219537 |
| detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method. | to culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ... | 2010 | 20219690 |
| potentiation of bacterial killing activity of zinc chloride by pyrrolidine dithiocarbamate. | zinc has antimicrobial activity and zinc salts including zinc chloride (zncl(2)) have been used for the control of oral malodor. in this study, we hypothesized that pyrrolidine dithiocarbamate (pdtc), a zinc ionophore, may enhance antimicrobial efficacy of zncl(2). the bactericidal effectiveness of zncl(2) alone (0.5-8 mm) or in combination with pdtc (1 or 10 microm) was evaluated by in vitro short (1 h) time-killing assays against fusobacterium nucleatum and porphyromonas gingivalis. only a sli ... | 2010 | 20221728 |
| antimicrobial effect of rosa damascena extract on selected endodontic pathogens. | the purpose of this study was to compare the antimicrobial activity of a plant-derived extract (2 percent rosa damascena extract) with 5.25 percent sodium hypochlorite (naocl) and 2 percent chlorhexidine (chx) on selected endodontic pathogens. the minimum inhibitory concentrations (mics) of 2 percent rose extract and 2 percent chx for test microorganisms, except f. nucleatum, were lower than that of 5.25 percent naocl. all solutions were able to kill all test microorganisms after one minute. | 2010 | 20232691 |
| photodynamic therapy in periodontal therapy: microbiological observations from a private practice. | in recent years, the combination of laser light and photosensitizer known as photodynamic therapy (pdt) has been used in periodontal therapy. however, there are not enough clinical studies to fully evaluate the effects of pdt on the periodontal tissues. this microbiological study examined the effects of pdt on the periodontal bacteria in combination with scaling and root planing (srp) in the same group of patients by randomly selecting pdt or srp for use in different quadrants of the mouth. for ... | 2010 | 20236906 |
| effect of alkaline growth ph on the expression of cell envelope proteins in fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative anaerobic organism that plays a central role in the development of periodontal diseases. the progression of periodontitis is associated with a rise in ph of the gingival sulcus which promotes the growth and expression of virulence factors by periodontopathic bacteria. we have previously reported that the expression of specific cytoplasmic proteins is altered by a shift in growth ph. in the present study we have compared cell envelope protein expression ... | 2010 | 20299401 |
| endodontic photoactivated disinfection using a conventional light source: an in vitro and ex vivo study. | the antimicrobial effect of photoactivated disinfection (pad) using toluidine blue and an led lamp was tested on endodontic pathogens in planktonic suspension and after inoculation into extracted teeth. irradiation time was limited to 30 seconds. | 2010 | 20303060 |
| a novel bioactivity of omega-3 polyunsaturated fatty acids and their ester derivatives. | fish oil, enriched in omega-3 polyunsaturated fatty acids (n-3 pufa), is widely used as a dietary or nutritional supplement with numerous benefits, including as an anti-inflammatory particularly linked to atherosclerosis. while n-3 pufa have been suggested to be able to improve oral health through a reduction in inflammation through elevations in these fatty acids in serum and cellular membranes, information is lacking for the possibility that these fatty acids could directly impact the survival ... | 2010 | 20331795 |
| supernatants from oral epithelial cells and gingival fibroblasts modulate human immunodeficiency virus type 1 promoter activation induced by periodontopathogens in monocytes/macrophages. | bacterial and host cell products during coinfections of human immunodeficiency virus type 1-positive (hiv-1(+)) patients regulate hiv-1 recrudescence in latently infected cells (e.g. t cells, monocytes/macrophages), impacting highly active antiretroviral therapy (haart) failure and progression of acquired immunodeficiency syndrome. a high frequency of oral opportunistic infections (e.g. periodontitis) in hiv-1(+) patients has been demonstrated; however, their potential to impact hiv-1 exacerbati ... | 2010 | 20331801 |
| full-mouth antimicrobial photodynamic therapy in fusobacterium nucleatum-infected periodontitis patients. | the purpose of this study is to clinically and microbiologically evaluate the effect of photodynamic therapy (pdt) as a full-mouth procedure in fusobacterium nucleatum-infected patients with periodontitis. | 2010 | 20350153 |
| biofilm formation enhances the oxygen tolerance and invasiveness of fusobacterium nucleatum in an oral mucosa culture model. | the present study evaluates the survival capability of fusobacterium nucleatum strains in an aerobic environment and compares the invasive capability of f. nucleatum in biofilm and planktonic forms in an organotypic cell culture (occ) model. | 2010 | 20350156 |
| case records of the massachusetts general hospital. case 10-2010. a 37-year-old woman with weakness and a mass in the brain. | | 2010 | 20375410 |
| mixture of periodontopathogenic bacteria influences interaction with kb cells. | the purpose of this study was to investigate the adhesion and invasion of periodontopathogenic bacteria in varied mixed infections and the release of interleukins from an epithelial cell line (kb cells). | 2010 | 20380884 |
| antimicrobial activity of super-oxidised water against oral microorganisms. | the radical anion of oxygen (o(-)) is extremely oxidative and shows high reactivity. in this study, the antibacterial activity of water super-oxidised water containing high concentration of o(-) (o(-)-water) was tested against cultured planktonic cells of cariogenic bacteria, periodontopathic bacteria and candida albicans. | 2010 | 20381790 |
| detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16s rrna gene pcr. | we describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16s rrna gene pcr. simultaneous detection of fusobacterium nucleatum and porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16s rrna genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis. | 2010 | 20392909 |
| [effect of periodontal mechanical treatment on periodontal pathogenic bacteria in gingival crevicular fluid of chronic periodontitis patients]. | to evaluate the subgingival prevalent rates of 6 periodontal pathogenic bacteria in gingival crevicular fluids of cp patients before and after treatment, to analyze the relationship between the prevalent variance and periodontal clinical parameters, and to provide a microbiologic method of evaluating curative effect and estimating the prognosis. | 2010 | 20396365 |
| production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in fusobacterium nucleatum subsp. nucleatum atcc 25586. | fusobacterium nucleatum produces a large amount of the toxic metabolite hydrogen sulfide in the oral cavity. here, we report the molecular basis of f. nucleatum h(2)s production, which is associated with two different enzymes: the previously reported cdl (fn1220) and the newly identified lcd (fn0625). sds-page analysis with activity staining revealed that crude enzyme extracts from f. nucleatum atcc 25586 contained three major h(2)s-producing proteins. two of the proteins with low molecular mass ... | 2010 | 20413556 |
| innate inflammatory responses of human decidual cells to periodontopathic bacteria. | the purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua. | 2010 | 20452492 |
| communication: antimicrobial activity of smap28 with a targeting domain for porphyromonas gingivalis. | antibiotic therapy is often used with mechanical therapy to treat periodontal disease. however, complications associated with antibiotic use can occur. a 'bacteria-specific' targeted approach would eliminate some of these complications and kill specific periodontopathogens without harming the commensal bacteria. one such approach is to couple antimicrobial peptides to a ligand, pheromone, or antibody specific for the periodontopathogen, porphyromonas gingivalis. to assess the feasibility of this ... | 2010 | 20454638 |
| antibacterial effects of mdpb against anaerobes associated with endodontic infections. | to investigate the antibacterial effects of 12-methacryloyloxydodecylpyridinium bromide (mdpb), an antibacterial monomer synthesized by combining quaternary ammonium with a methacryloyl group, against three anaerobes associated with endodontic infections using planktonic and biofilm cells. | 2010 | 20456514 |
| production of indole from l-tryptophan and effects of these compounds on biofilm formation by fusobacterium nucleatum atcc 25586. | the l-tryptophan degradation product indole is a purported extracellular signaling molecule that influences biofilm formation in various bacteria. here we analyzed the mechanisms of indole production in fusobacterium nucleatum and the effects of tryptophan and indole on f. nucleatum planktonic and biofilm cells. the amino acid sequence deduced from the fn1943 gene in f. nucleatum atcc 25586 was 28% identical to that deduced from tnaa in escherichia coli, which encodes tryptophanase catalyzing th ... | 2010 | 20472741 |
| competition between yogurt probiotics and periodontal pathogens in vitro. | to investigate the competition between probiotics in bio-yogurt and periodontal pathogens in vitro. | 2010 | 20491536 |
| identification of immunological parameters associated with the alveolar bone level in periodontal patients. | the present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum igg titer against periodontopathogenic bacteria, the expression of the helper t-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (hsp) epitope peptide from p. gingivalis hsp60. | 2010 | 20498762 |
| in vitro cytokine responses to periodontal pathogens: generalized aggressive periodontitis is associated with increased il-6 response to porphyromonas gingivalis. | generalized aggressive periodontitis (gagp) is an inflammatory condition resulting in destruction of tooth-supporting tissues. we examined the production of il-1beta, il-6, tumour necrosis factor (tnf)-alpha, il-12 and il-10 in cultures of peripheral mononuclear cells (mnc) from 10 patients with gagp and 10 controls stimulated with periodontal pathogens or a control antigen, tetanus toxoid (tt) in the presence of autologous serum. the pathogens used were porphyromonas gingivalis, prevotella inte ... | 2010 | 20500696 |
| the recolonization hypothesis in a full-mouth or multiple-session treatment protocol: a blinded, randomized clinical trial. | to test recolonization of periodontal lesions after full-mouth scaling and root planing (fm-srp) or multiple session-srp (ms-srp) in a randomized clinical trial and whether fm-srp and ms-srp result in different clinical outcomes. | 2010 | 20507375 |
| mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. ii. microbiological results. | peri-implantitis is common in patients with dental implants. we performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions. | 2010 | 20507380 |
| [reversible metronidazole-induced encephalopathy and hypertrophic olivary degeneration]. | | 2010 | 20508563 |
| effects of a composition containing lactoferrin and lactoperoxidase on oral malodor and salivary bacteria: a randomized, double-blind, crossover, placebo-controlled clinical trial. | we report a clinical trial of the effects of test tablets containing bovine lactoferrin and lactoperoxidase on oral malodor and salivary bacteria. fifteen subjects with volatile sulfur compounds (vscs) in mouth air above the olfactory threshold (h(2)s >1.5 or ch(3)sh >0.5 ng/10 ml) as detected by gas chromatography were enrolled in the trial. either a test or a placebo tablet was ingested twice at 1-h intervals in two crossover phases. mouth air was monitored for vsc levels at the baseline befor ... | 2010 | 20512389 |
| antimicrobial efficacy of 3 oral antiseptics containing octenidine, polyhexamethylene biguanide, or citroxx: can chlorhexidine be replaced? | use of oral antiseptics decreases the bacterial load in the oral cavity. | 2010 | 20518635 |
| gram-negative periodontal bacteria induce the activation of toll-like receptors 2 and 4, and cytokine production in human periodontal ligament cells. | periodontitis is a bacterially induced chronic inflammatory disease. toll-like receptors (tlrs), which could recognize microbial pathogens, are important components in the innate and adaptive immune systems. both qualitatively and quantitatively distinct immune responses might result from different bacteria stimulation and the triggering of different tlrs. this study explores the interaction of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, and aggregatibacter actinomy ... | 2010 | 20528699 |
| folding of a transcriptionally acting preq1 riboswitch. | 7-aminomethyl-7-deazaguanine (preq(1)) sensitive mrna domains belong to the smallest riboswitches known to date. although recent efforts have revealed the three-dimensional architecture of the ligand-aptamer complex less is known about the molecular details of the ligand-induced response mechanism that modulates gene expression. we present an in vitro investigation on the ligand-induced folding process of the preq(1) responsive rna element from fusobacterium nucleatum using biophysical methods, ... | 2010 | 20534493 |
| antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms. | this study is to assess the antibacterial activity of omega-6, -7, -9 (n-6, n-7, n-9) fatty acids against various oral microorganisms. | 2010 | 20541177 |
| antibiotic resistance of subgingival species in chronic periodontitis patients. | the increasing rate of resistance of microorganisms to penicillin and other antibiotics has generated concern among health authorities in latin america. the present investigation determined the in vitro susceptibility of porphyromonas gingivalis, fusobacterium nucleatum, black-pigmented prevotella spp. and aggregatibacter actinomycetemcomitans to metronidazole, amoxicillin, amoxicillin/clavulanic acid, clindamycin and moxifloxacin in patients with chronic periodontitis. | 2010 | 20546113 |
| [usefulness and limit of gram staining smear examination]. | gram staining is one of the most simple and inexpensive methods for the rapid diagnosis of bacterial and fungal infections. it yields results much faster than culture, and provides important data for the patient's treatment and prognosis. however, a difference exists in the quality and quantity of information yielded by gram staining smears based on the experience and knowledge of those conducting the tests. therefore, a risk of misdiagnosis based on the information obtained from gram staining s ... | 2010 | 20560458 |
| hiv-1 reactivation induced by the periodontal pathogens fusobacterium nucleatum and porphyromonas gingivalis involves toll-like receptor 2 [corrected] and 9 activation in monocytes/macrophages. | although oral coinfections (e.g., periodontal disease) are highly prevalent in human immunodeficiency virus type 1-positive (hiv-1(+)) patients and appear to positively correlate with viral load levels, the potential for oral bacteria to induce hiv-1 reactivation in latently infected cells has received little attention. we showed that hiv-1 long terminal repeat (ltr) promoter activation can be induced by periodontopathogens in monocytes/macrophages; nevertheless, the mechanisms involved in this ... | 2010 | 20610663 |
| differential and coordinated expression of defensins and cytokines by gingival epithelial cells and dendritic cells in response to oral bacteria. | epithelial cells and dendritic cells (dcs) both initiate and contribute to innate immune responses to bacteria. however, much less is known about the coordinated regulation of innate immune responses between gecs and immune cells, particularly dcs in the oral cavity. the present study was conducted to investigate whether their responses are coordinated and are bacteria-specific in the oral cavity. | 2010 | 20618959 |
| new aspects and new concepts of maintaining "microbiological" health. | the human mouth is host to a diverse collection of microorganisms including bacteria, viruses, fungi and protozoa. recent advances using molecular methods for the analysis of complex bacterial communities have demonstrated the richness of the oral bacterial biota and the presence of numerous previously undescribed lineages. dental plaque forms in a structured way with pioneer species able to colonise pellicle-coated enamel followed by secondary plaque formers such as fusobacterium nucleatum that ... | 2010 | 20621241 |
| antiaggregation potential of berry fractions against pairs of streptococcus mutans with fusobacterium nucleatum or actinomyces naeslundii. | coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. this is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kda, fi; 10-100 kda, fii; >100 kda, fiii) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: streptococcus mutans with fusobacterium nucleatum ... | 2011 | 20623601 |
| examination of periodontal pathogens in stenotic valve specimens and in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. | periodontitis may be a risk factor for atherosclerosis and coronary heart disease. the influence of periodontal pathogens in cardiovascular diseases needs further investigation. therefore, the aims of this clinical study are: to test the presence of periodontal bacteria dna in aortic valves and to assess the concomitant presence of the same periodontal bacteria dna in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. nineteen consecutive patients (12 ma ... | 2010 | 20646351 |
| microbiota around teeth and dental implants in periodontally healthy, partially edentulous patients: is pre-implant microbiological testing relevant? | this study aimed to assess the prevalence of seven periodontal marker pathogens, before implant placement and 1 yr after loading, in periodontally healthy individuals and to assess the long-term effectiveness of pre-implant reduction of pathogens to below threshold levels. in 93 individuals needing single tooth replacement, pooled subgingival microbiological samples from standard sites were cultured and analyzed before implant treatment and 1 yr after loading. threshold levels commonly used in p ... | 2010 | 20662908 |
| fusobacterium nucleatum regulation of neutrophil transcription. | abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum. | 2011 | 20663022 |
| energy dose parameters affect antimicrobial photodynamic therapy-mediated eradication of periopathogenic biofilm and planktonic cultures. | this study evaluated the in vitro efficacy of a commercially available apdt system in eradication of the periopathogens porphyromonas gingivalis, fusobacterium nucleatum, and aggregatibacter actinomycetemcomitans in both planktonic and biofilm cultures. | 2010 | 20666581 |
| vaccination of mice with porphyromonas gingivalis or fusobacterium nucleatum modulates the inflammatory response, but fails to prevent experimental periodontitis. | to assess the potential of using vaccination with porphyromonas gingivalis or fusobacterium nucleatum, in modulating local subcutaneous inflammatory response and alveolar bone loss following coinfection with both bacteria. | 2010 | 20670341 |
| a brain abscess following dental extractions in a patient with hereditary hemorrhagic telangiectasia. | oral and maxillofacial surgeons must be aware of the potentially life-threatening complications of dental extractions in patients with hereditary haemorrhagic telangiectasia because of their high prevalence of pulmonary arteriovenous fistulas. despite the lack of evidence-based guidelines, antibiotic cover should be given to patients with hht who require oral surgery according to the same rules as those used for patients at high risk of bacterial endocarditis. | 2010 | 20719417 |
| polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells. | to investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (dcs), at the mucosal surface. | 2011 | 20798974 |
| fusobacterium nucleatum outer membrane proteins fap2 and radd induce cell death in human lymphocytes. | bacterially induced cell death in human lymphocytes is an important virulence factor for pathogenic bacteria. previously discovered mechanisms of bacterially induced cell death are predominantly based on the transfer of bacterial proteins to the target host cell, such as the toxins secreted through type i, ii, and vi secretion systems or effector proteins injected through type iii, iv, and vb secretion systems. here, we report a mechanism employed by the gram-negative oral pathogen fusobacterium ... | 2010 | 20823215 |
| a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants. | supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues. | 2011 | 20831669 |
| fusobacterium nucleatum binding to complement regulatory protein cd46 modulates the expression and secretion of cytokines and matrix metalloproteinases by oral epithelial cells. | periodontitis is a chronic inflammatory disease that results in the destruction of the supporting tissues of the teeth. gingival epithelial cells are an important mechanical barrier and participate in the host inflammatory response to periodontopathogens. the aim of the present study is to investigate the capacity of fusobacterium nucleatum to bind to the complement regulatory protein cd46 expressed by oral epithelial cells and to determine the impact of the binding on the gene expression and pr ... | 2011 | 20843232 |
| fusobacterium nucleatum-associated beta-defensin inducer (fad-i): identification, isolation, and functional evaluation. | human β-defensins (hbds) are small, cationic antimicrobial peptides, secreted by mucosal epithelial cells that regulate adaptive immune functions. we previously reported that fusobacterium nucleatum, a ubiquitous gram-negative bacterium of the human oral cavity, induces human β-defensin 2 (hbd2) upon contact with primary oral epithelial cells. we now report the isolation and characterization of an f. nucleatum (atcc 25586)-associated defensin inducer (fad-i). biochemical approaches revealed a ce ... | 2010 | 20847052 |
| antimicrobial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria. | the aim of this study is to assess the antibacterial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria. | 2010 | 20849395 |
| apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis. | bacteria located in the apical root canal system potentially participate in the pathogenesis of apical periodontitis. detection and identification of apical bacteria can be compromised because of limitations in conventional sampling and identification procedures. this study identified several bacterial taxa in the apical and middle/coronal segments of primarily infected root canal system by using pulverized root segments and a culture-independent molecular method. | 2010 | 20850664 |
| isolation of a novel bacteriophage specific for the periodontal pathogen fusobacterium nucleatum. | fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. we isolated a new bacteriophage (fnpφ02) that specifically infects this bacterium. transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. the size of the phage genome was estimated to be approximately 59 kbp of double-stranded ... | 2010 | 20851973 |
| [effect of compound chinese traditional medicine on infected root canal bacteria biofilm]. | to assess the efficacy of compound chinese traditional medicine(ctm), which composed of gallic acid, magnolol and polysaccharide of blettila striata, against the infected root canal bacterial biofilm. | 2010 | 20871960 |