| erythritol feeds the pentose phosphate pathway via three new isomerases leading to d-erythrose-4-phosphate in brucella. | erythritol is an important nutrient for several α-2 proteobacteria, including n2-fixing plant endosymbionts and brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues. erythritol metabolism involves phosphorylation to l-erythritol-4-phosphate by the kinase erya and oxidation of the latter to l-3-tetrulose 4-phosphate by the dehydrogenase eryb. it is accepted that further steps involve oxidation by the putative dehydrogenase eryc and subsequent decarboxylation to yie ... | 2014 | 25453104 |
| molecular characterization of a novel temperate sinorhizobium bacteriophage, фlm21, encoding dna methyltransferase with ccrm-like specificity. | φlm21 is a temperate phage isolated from sinorhizobium sp. strain lm21 (alphaproteobacteria). genomic analysis and electron microscopy suggested that φlm21 is a member of the family siphoviridae. the phage has an isometric head and a long noncontractile tail. the genome of φlm21 has 50,827 bp of linear double-stranded dna encoding 72 putative proteins, including proteins responsible for the assembly of the phage particles, dna packaging, transcription, replication, and lysis. virion proteins wer ... | 2014 | 25187538 |
| profound impact of hfq on nutrient acquisition, metabolism and motility in the plant pathogen agrobacterium tumefaciens. | as matchmaker between mrna and srna interactions, the rna chaperone hfq plays a key role in riboregulation of many bacteria. often, the global influence of hfq on the transcriptome is reflected by substantially altered proteomes and pleiotropic phenotypes in hfq mutants. using quantitative proteomics and co-immunoprecipitation combined with rna-sequencing (rip-seq) of hfq-bound rnas, we demonstrate the pervasive role of hfq in nutrient acquisition, metabolism and motility of the plant pathogen a ... | 2014 | 25330313 |
| a moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors. | there are a paucity of data concerning gene products that could contribute to the ability of moraxella catarrhalis to colonize the human nasopharynx. inactivation of a gene (mesr) encoding a predicted response regulator of a two-component signal transduction system in m. catarrhalis yielded a mutant unable to grow in liquid media. this mesr mutant also exhibited increased sensitivity to certain stressors, including polymyxin b, sds, and hydrogen peroxide. inactivation of the gene (mess) encoding ... | 2014 | 25312959 |
| a moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors. | there are a paucity of data concerning gene products that could contribute to the ability of moraxella catarrhalis to colonize the human nasopharynx. inactivation of a gene (mesr) encoding a predicted response regulator of a two-component signal transduction system in m. catarrhalis yielded a mutant unable to grow in liquid media. this mesr mutant also exhibited increased sensitivity to certain stressors, including polymyxin b, sds, and hydrogen peroxide. inactivation of the gene (mess) encoding ... | 2014 | 25312959 |
| characterization and evaluation of an arbitrary primed polymerase chain reaction (pcr) product for the specific detection of brucella species. | laboratory detection of brucella is based largely on bacterial isolation and phenotypic characterization. these methods are lengthy and labor-intensive and have been associated with a heightened risk of laboratory-acquired infection. antibody based indirect detection methods also suffer from limitations in proper diagnosis of the organism. to overcome these problems, nucleic acid amplification has been explored for rapid detection and confirmation of the presence of brucella spp. pcr-based diagn ... | 2014 | 25737656 |
| characterization and evaluation of an arbitrary primed polymerase chain reaction (pcr) product for the specific detection of brucella species. | laboratory detection of brucella is based largely on bacterial isolation and phenotypic characterization. these methods are lengthy and labor-intensive and have been associated with a heightened risk of laboratory-acquired infection. antibody based indirect detection methods also suffer from limitations in proper diagnosis of the organism. to overcome these problems, nucleic acid amplification has been explored for rapid detection and confirmation of the presence of brucella spp. pcr-based diagn ... | 2014 | 25737656 |
| two separate modules of the conserved regulatory rna abcr1 address multiple target mrnas in and outside of the translation initiation region. | the small rna abcr1 regulates the expression of abc transporters in the plant pathogen agrobacterium tumefaciens, the plant symbiont sinorhizobium meliloti, and the human pathogen brucella abortus. a combination of proteomic and bioinformatic approaches suggested dozens of abcr1 targets in a. tumefaciens. several of these newly discovered targets are involved in the uptake of amino acids, their derivatives, and sugars. among the latter is the periplasmic sugar-binding protein chve, a component o ... | 2014 | 24921646 |
| mechanisms and regulation of surface interactions and biofilm formation in agrobacterium. | for many pathogenic bacteria surface attachment is a required first step during host interactions. attachment can proceed to invasion of host tissue or cells or to establishment of a multicellular bacterial community known as a biofilm. the transition from a unicellular, often motile, state to a sessile, multicellular, biofilm-associated state is one of the most important developmental decisions for bacteria. agrobacterium tumefaciens genetically transforms plant cells by transfer and integratio ... | 2014 | 24834068 |
| differential control of bradyrhizobium japonicum iron stimulon genes through variable affinity of the iron response regulator (irr) for target gene promoters and selective loss of activator function. | bradyrhizobium japonicum irr is a conditionally stable transcriptional activator and repressor that accumulates in cells under iron-limited, manganese-replete conditions, but degrades in a haem-dependent manner under high iron conditions, manganese limitation or upon exposure to h2 o2 . here, we identified irr-regulated genes that were relatively unresponsive to factors that promote irr degradation. the promoters of those genes bound irr with at least 200-fold greater affinity than promoters of ... | 2014 | 24646221 |
| a signaling pathway involving the diguanylate cyclase celr and the response regulator divk controls cellulose synthesis in agrobacterium tumefaciens. | the production of cellulose fibrils is involved in the attachment of agrobacterium tumefaciens to its plant host. consistent with previous studies, we reported recently that a putative diguanylate cyclase, celr, is required for synthesis of this polymer in a. tumefaciens. in this study, the effects of celr and other components of the regulatory pathway of cellulose production were explored. mutational analysis of celr demonstrated that the cyclase requires the catalytic ggeef motif, as well as t ... | 2014 | 24443526 |
| the ctra phosphorelay integrates differentiation and communication in the marine alphaproteobacterium dinoroseobacter shibae. | dinoroseobacter shibae, a member of the roseobacter clade abundant in marine environments, maintains morphological heterogeneity throughout growth, with small cells dividing by binary fission and large cells dividing by budding from one or both cell poles. this morphological heterogeneity is lost if the quorum sensing (qs) system is silenced, concurrent with a decreased expression of the ctra phosphorelay, a regulatory system conserved in alphaproteobacteria and the master regulator of the caulo ... | 2014 | 24524855 |
| from plant infectivity to growth patterns: the role of blue-light sensing in the prokaryotic world. | flavin-based photoreceptor proteins of the lov (light, oxygen, and voltage) and bluf (blue light sensing using flavins) superfamilies are ubiquitous among the three life domains and are essential blue-light sensing systems, not only in plants and algae, but also in prokaryotes. here we review their biological roles in the prokaryotic world and their evolution pathways. an unexpected large number of bacterial species possess flavin-based photosensors, amongst which are important human and plant p ... | 2014 | 27135492 |
| phototropism: some history, some puzzles, and a look ahead. | | 2014 | 24399823 |
| an update on iron acquisition by legionella pneumophila: new pathways for siderophore uptake and ferric iron reduction. | iron acquisition is critical for the growth and pathogenesis of legionella pneumophila, the causative agent of legionnaires' disease. l. pneumophila utilizes two main modes of iron assimilation, namely ferrous iron uptake via the feob system and ferric iron acquisition through the action of the siderophore legiobactin. this review highlights recent studies concerning the mechanism of legiobactin assimilation, the impact of c-type cytochromes on siderophore production, the importance of legiobact ... | 2015 | 26000653 |
| thermodynamic matchers for the construction of the cuckoo rna family. | rna family models describe classes of functionally related, non-coding rnas based on sequence and structure conservation. the most important method for modeling rna families is the use of covariance models, which are stochastic models that serve in the discovery of yet unknown, homologous rnas. however, the performance of covariance models in finding remote homologs is poor for rna families with high sequence conservation, while for families with high structure but low sequence conservation, the ... | 2015 | 25779873 |
| effect of nutrient periodicity on microbial community dynamics. | when microbes are subjected to temporal changes in nutrient availability, growth rate and substrate affinity can contribute to competitive fitness and thereby affect microbial community structure. this hypothesis was tested using planktonic bacterial communities exposed to nutrient additions at 1-, 3-, 7-, or 14-day intervals. growth rates after nutrient addition were inversely proportional to the pulse interval and declined from 0.5 h(-1) to 0.15 h(-1) as the pulse interval increased from 1 to ... | 2006 | 16672455 |
| biochemistry and genetics of acc deaminase: a weapon to "stress ethylene" produced in plants. | 1-aminocyclopropane-1-carboxylate deaminase (accd), a pyridoxal phosphate-dependent enzyme, is widespread in diverse bacterial and fungal species. owing to accd activity, certain plant associated bacteria help plant to grow under biotic and abiotic stresses by decreasing the level of "stress ethylene" which is inhibitory to plant growth. accd breaks down acc, an immediate precursor of ethylene, to ammonia and α-ketobutyrate, which can be further metabolized by bacteria for their growth. acc deam ... | 2015 | 26441873 |
| metagenomic analysis of streptomyces lividans reveals host-dependent functional expression. | most functional metagenomic studies have been limited by the poor expression of many genes derived from metagenomic dna in escherichia coli, which has been the predominant surrogate host to date. to expand the range of expressed genes, we developed tools for construction and functional screening of metagenomic libraries in streptomyces lividans. we expanded on previously published protocols by constructing a system that enables retrieval and characterization of the metagenomic dna from biologica ... | 2012 | 22427497 |
| plant growth promotion potential is equally represented in diverse grapevine root-associated bacterial communities from different biopedoclimatic environments. | plant-associated bacteria provide important services to host plants. environmental factors such as cultivar type and pedoclimatic conditions contribute to shape their diversity. however, whether these environmental factors may influence the plant growth promoting (pgp) potential of the root-associated bacteria is not widely understood. to address this issue, the diversity and pgp potential of the bacterial assemblage associated with the grapevine root system of different cultivars in three medit ... | 2013 | 23878810 |
| a positive correlation between bacterial autoaggregation and biofilm formation in native sinorhizobium meliloti isolates from argentina. | sinorhizobium meliloti is a symbiotic nitrogen-fixing bacterium that elicits nodule formation on roots of alfalfa plants. s. meliloti produces two exopolysaccharides (epss), termed eps i and eps ii, that are both able to promote symbiosis. eps i and eps ii are secreted in two major fractions that reflect differing degrees of subunit polymerization, designated high- and low-molecular-weight fractions. we reported previously that epss are crucial for autoaggregation and biofilm formation in s. mel ... | 2012 | 22492433 |
| the type vi secretion toolkit. | bacterial secretion systems are macromolecular complexes that release virulence factors into the medium or translocate them into the target host cell. these systems are widespread in bacteria allowing them to infect eukaryotic cells and survive or replicate within them. a new secretion system, the type vi secretion system (t6ss), was recently described and characterized in several pathogens. genomic data suggest that t6ss exist in most bacteria that come into close contact with eukaryotic cells, ... | 2008 | 18617888 |
| dissecting the bacterial type vi secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? | the availability of hundreds of bacterial genomes allowed a comparative genomic study of the type vi secretion system (t6ss), recently discovered as being involved in pathogenesis. by combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global t6ss genetic structure in terms of conservation, evolution and genomic organization. | 2009 | 19284603 |
| mucosal immunization with live attenuated francisella novicida u112δiglb protects against pulmonary f. tularensis schu s4 in the fischer 344 rat model. | the need for an efficacious vaccine against francisella tularensis is a consequence of its low infectious dose and high mortality rate if left untreated. this study sought to characterize a live attenuated subspecies novicida-based vaccine strain (u112δiglb) in an established second rodent model of pulmonary tularemia, namely the fischer 344 rat using two distinct routes of vaccination (intratracheal [i.t.] and oral). attenuation was verified by comparing replication of u112δiglb with wild type ... | 2012 | 23118885 |
| genome of the r-body producing marine alphaproteobacterium labrenzia alexandrii type strain (dfl-11(t)). | labrenzia alexandrii biebl et al. 2007 is a marine member of the family rhodobacteraceae in the order rhodobacterales, which has thus far only partially been characterized at the genome level. the bacterium is of interest because it lives in close association with the toxic dinoflagellate alexandrium lusitanicum. ultrastructural analysis reveals r-bodies within the bacterial cells, which are primarily known from obligate endosymbionts that trigger "killing traits" in ciliates (paramecium spp.). ... | 2013 | 24019989 |
| characterization of tetratricopeptide repeat-like proteins in francisella tularensis and identification of a novel locus required for virulence. | francisella tularensis is a highly infectious bacterium that causes the potentially lethal disease tularemia. this extremely virulent bacterium is able to replicate in the cytosolic compartments of infected macrophages. to invade macrophages and to cope with their intracellular environment, francisella requires multiple virulence factors, which are still being identified. proteins containing tetratricopeptide repeat (tpr)-like domains seem to be promising targets to investigate, since these prot ... | 2014 | 25245806 |
| francisella novicida pathogenicity island encoded proteins were secreted during infection of macrophage-like cells. | intracellular pathogens and other organisms have evolved mechanisms to exploit host cells for their life cycles. virulence genes of some intracellular bacteria responsible for these mechanisms are located in pathogenicity islands, such as secretion systems that secrete effector proteins. the francisella pathogenicity island is required for phagosomal escape, intracellular replication, evasion of host immune responses, virulence, and encodes a type 6 secretion system. we hypothesize that some fra ... | 2014 | 25158041 |
| the moxr atpase rava and its cofactor viaa interact with the nadh:ubiquinone oxidoreductase i in escherichia coli. | moxr atpases are widespread throughout bacteria and archaea. the experimental evidence to date suggests that these proteins have chaperone-like roles in facilitating the maturation of dedicated protein complexes that are functionally diverse. in escherichia coli, the moxr atpase rava and its putative cofactor viaa are found to exist in early stationary-phase cells at 37 °c at low levels of about 350 and 90 molecules per cell, respectively. both proteins are predominantly localized to the cytopla ... | 2014 | 24454883 |
| fha interaction with phosphothreonine of tssl activates type vi secretion in agrobacterium tumefaciens. | the type vi secretion system (t6ss) is a widespread protein secretion system found in many gram-negative bacteria. t6sss are highly regulated by various regulatory systems at multiple levels, including post-translational regulation via threonine (thr) phosphorylation. the ser/thr protein kinase ppka is responsible for this thr phosphorylation regulation, and the forkhead-associated (fha) domain-containing fha-family protein is the sole t6ss phosphorylation substrate identified to date. here we d ... | 2014 | 24626341 |
| modification of the 1-phosphate group during biosynthesis of capnocytophaga canimorsus lipid a. | capnocytophaga canimorsus, a commensal bacterium of dog's mouth flora causing severe infections in humans after dog bites or scratches, has a lipopolysaccharide (lps) (endotoxin) with low-inflammatory lipid a. in particular, it contains a phosphoethanolamine (p-etn) instead of a free phosphate group at the c-1 position of the lipid a backbone, usually present in highly toxic enterobacterial gram-negative lipid a. here we show that the c. canimorsus genome comprises a single operon encoding a lip ... | 2016 | 26644381 |
| dual role of hupf in the biosynthesis of [nife] hydrogenase in rhizobium leguminosarum. | [nife] hydrogenases are enzymes that catalyze the oxidation of hydrogen into protons and electrons, to use h₂ as energy source, or the production of hydrogen through proton reduction, as an escape valve for the excess of reduction equivalents in anaerobic metabolism. biosynthesis of [nife] hydrogenases is a complex process that occurs in the cytoplasm, where a number of auxiliary proteins are required to synthesize and insert the metal cofactors into the enzyme structural units. the endosymbioti ... | 2012 | 23136881 |
| bacterial iron-sulfur regulatory proteins as biological sensor-switches. | in recent years, bacterial iron-sulfur cluster proteins that function as regulators of gene transcription have emerged as a major new group. in all cases, the cluster acts as a sensor of the environment and enables the organism to adapt to the prevailing conditions. this can range from mounting a response to oxidative or nitrosative stress to switching between anaerobic and aerobic respiratory pathways. the sensitivity of these ancient cofactors to small molecule reactive oxygen and nitrogen spe ... | 2012 | 22239203 |
| the gtar protein negatively regulates transcription of the gtari operon and modulates gene transfer agent (rcgta) expression in rhodobacter capsulatus. | the gtai gene of rhodobacter capsulatus encodes an n-acyl-homoserine lactone (acyl-hsl) synthase. immediately 5' of the gtai gene is orf rcc00328 that encodes a potential acyl-hsl receptor protein. a combination of genetic and biochemical approaches showed that rcc00328 (renamed gtar) modulates the production of a genetic exchange element called the gene transfer agent (rcgta), and regulates the transcription of gtai. although gtai mutants exhibited decreased levels of rcgta production, mutagene ... | 2012 | 22211723 |
| association between chloroplast and mitochondrial dna sequences in chinese prunus genotypes (prunus persica, prunus domestica, and prunus avium). | the nuclear dna is conventionally used to assess the diversity and relatedness among different species, but variations at the dna genome level has also been used to study the relationship among different organisms. in most species, mitochondrial and chloroplast genomes are inherited maternally; therefore it is anticipated that organelle dna remains completely associated. many research studies were conducted simultaneously on organelle genome. the objectives of this study was to analyze the genet ... | 2015 | 25592231 |
| (meta)genomic insights into the pathogenome of cellulosimicrobium cellulans. | despite having serious clinical manifestations, cellulosimicrobium cellulans remain under-reported with only three genome sequences available at the time of writing. genome sequences of c. cellulans lmg16121, c. cellulans j36 and cellulosimicrobium sp. strain mm were used to determine distribution of pathogenicity islands (pais) across c. cellulans, which revealed 49 potential marker genes with known association to human infections, e.g. fic and vbha toxin-antitoxin system. oligonucleotide compo ... | 2016 | 27151933 |
| microbial enzymes: tools for biotechnological processes. | microbial enzymes are of great importance in the development of industrial bioprocesses. current applications are focused on many different markets including pulp and paper, leather, detergents and textiles, pharmaceuticals, chemical, food and beverages, biofuels, animal feed and personal care, among others. today there is a need for new, improved or/and more versatile enzymes in order to develop more novel, sustainable and economically competitive production processes. microbial diversity and m ... | 2014 | 24970208 |
| mining the sinorhizobium meliloti transportome to develop fret biosensors for sugars, dicarboxylates and cyclic polyols. | förster resonance energy transfer (fret) biosensors are powerful tools to detect biologically important ligands in real time. currently fret bisosensors are available for twenty-two compounds distributed in eight classes of chemicals (two pentoses, two hexoses, two disaccharides, four amino acids, one nucleobase, two nucleotides, six ions and three phytoestrogens). to expand the number of available fret biosensors we used the induction profile of the sinorhizobium meliloti transportome to system ... | 2012 | 23028462 |
| molecular characterization of nad+-dependent dna ligase from wolbachia endosymbiont of lymphatic filarial parasite brugia malayi. | the lymphatic filarial parasite, brugia malayi contains wolbachia endobacteria that are essential for development, viability and fertility of the parasite. therefore, wolbachial proteins have been currently seen as the potential antifilarial drug targets. nad(+)-dependent dna ligase is characterized as a promising drug target in several organisms due to its crucial, indispensable role in dna replication, recombination and dna repair. we report here the cloning, expression and purification of nad ... | 2012 | 22815933 |
| insights into protein allostery in the csor/rcnr family of transcriptional repressors. | csor/rcnr transcriptional repressors adopt a disc-shaped, all α-helical dimer of dimers tetrameric architecture, with a four-helix bundle the key structural feature of the dimer. individual members of this large family of repressors coordinate cu(i) or ni(ii)/co(ii) or perform cysteine sulfur chemistry in mitigating the effects of metal or metabolite toxicity, respectively. here we highlight recent insights into the functional diversity of this fascinating family of repressors. | 2013 | 24695963 |
| insights into protein allostery in the csor/rcnr family of transcriptional repressors. | csor/rcnr transcriptional repressors adopt a disc-shaped, all α-helical dimer of dimers tetrameric architecture, with a four-helix bundle the key structural feature of the dimer. individual members of this large family of repressors coordinate cu(i) or ni(ii)/co(ii) or perform cysteine sulfur chemistry in mitigating the effects of metal or metabolite toxicity, respectively. here we highlight recent insights into the functional diversity of this fascinating family of repressors. | 2013 | 24695963 |
| crystallization and preliminary x-ray study of alanine dehydrogenase from bacillus pseudofirmus of4. | alanine dehydrogenase (of4ald) from the alkaliphilic bacillus pseudofirmus of4 was expressed and purified with a his6 tag in a form suitable for x-ray crystallographic analysis. crystals were grown by the hanging-drop vapour-diffusion method at 289 k using a solution consisting of 0.1 m tris-hcl ph 8.0, 0.2 m liso4, 22%(w/v) peg 3350. x-ray diffraction data were collected to 2.8 å resolution. the crystal belonged to the triclinic space group p1, with unit-cell parameters a = 88.04, b = 105.59, c ... | 2013 | 24192355 |
| metagenomics and novel gene discovery: promise and potential for novel therapeutics. | metagenomics provides a means of assessing the total genetic pool of all the microbes in a particular environment, in a culture-independent manner. it has revealed unprecedented diversity in microbial community composition, which is further reflected in the encoded functional diversity of the genomes, a large proportion of which consists of novel genes. herein, we review both sequence-based and functional metagenomic methods to uncover novel genes and outline some of the associated problems of e ... | 2013 | 24317337 |
| metagenomics and novel gene discovery: promise and potential for novel therapeutics. | metagenomics provides a means of assessing the total genetic pool of all the microbes in a particular environment, in a culture-independent manner. it has revealed unprecedented diversity in microbial community composition, which is further reflected in the encoded functional diversity of the genomes, a large proportion of which consists of novel genes. herein, we review both sequence-based and functional metagenomic methods to uncover novel genes and outline some of the associated problems of e ... | 2013 | 24317337 |
| a unique uracil-dna binding protein of the uracil dna glycosylase superfamily. | uracil dna glycosylases (udgs) are an important group of dna repair enzymes, which pioneer the base excision repair pathway by recognizing and excising uracil from dna. based on two short conserved sequences (motifs a and b), udgs have been classified into six families. here we report a novel udg, udgx, from mycobacterium smegmatis and other organisms. udgx specifically recognizes uracil in dna, forms a tight complex stable to sodium dodecyl sulphate, 2-mercaptoethanol, urea and heat treatment, ... | 2015 | 26304551 |
| functional characterization of a csor-cuea divergon in bradyrhizobium liaoningense ccnwsx0360, involved in copper, zinc and cadmium cotolerance. | random mutagenesis in a symbiotic nitrogen-fixing bradyrhizobium liaoningense ccnwsx0360 (bln0360) using tn5 identified five copper (cu) resistance-related genes. they were functionally sorted into three groups: transmembrane transport (cuea and tolc); oxidation (copa); and protection of the membrane barrier (lpte and ctpa). the gene cuea, together with the upstream csor (cu-sensitive operon repressor), constituted a csor-cuea divergon which plays a crucial role in cu homeostasis. deletion of cu ... | 2016 | 27725778 |
| bioprospecting sponge-associated microbes for antimicrobial compounds. | sponges are the most prolific marine organisms with respect to their arsenal of bioactive compounds including antimicrobials. however, the majority of these substances are probably not produced by the sponge itself, but rather by bacteria or fungi that are associated with their host. this review for the first time provides a comprehensive overview of antimicrobial compounds that are known to be produced by sponge-associated microbes. we discuss the current state-of-the-art by grouping the bioact ... | 2016 | 27144573 |
| horizontal gene transfer and diverse functional constrains within a common replication-partitioning system in alphaproteobacteria: the repabc operon. | the repabc plasmid family, which is extensively present within alphaproteobacteria, and some secondary chromosomes of the rhizobiales have the particular feature that all the elements involved in replication and partitioning reside within one transcriptional unit, the repabc operon. given the functional interactions among the elements of the repabc operon, and the fact that they all reside in the same operon, a common evolutionary history would be expected if the entire operon had been horizonta ... | 2009 | 19919719 |
| characterization of the flavonoid-responsive regulator frra and its binding sites. | previous microarray analyses revealed that in bradyrhizobium japonicum, about 100 genes are induced by genistein, an isoflavonoid secreted by soybean. this includes the three genes frec, frea, and freb (systematic designations bll4319, bll4320, and bll4321), which are likely to form a genistein-, daidzein-, and coumestrol-inducible operon and to encode a multidrug efflux system. upstream of frecab and in the opposite orientation, frra (systematic designation blr4322), which has similarity to tet ... | 2012 | 22389485 |
| mercury reduction and methyl mercury degradation by the soil bacterium xanthobacter autotrophicus py2. | two previously uncharacterized potential broad-spectrum mercury (hg) resistance operons (mer) are present on the chromosome of the soil alphaproteobacteria xanthobacter autotrophicus py2. these operons, mer1 and mer2, contain two features which are commonly found in mer operons in the genomes of soil and marine alphaproteobacteria, but are not present in previously characterized mer operons: a gene for the mercuric reductase (mera) that encodes an alkylmercury lyase domain typical of those found ... | 2015 | 26341208 |
| analysis of facultative lithotroph distribution and diversity on volcanic deposits by use of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase. | a 492- to 495-bp fragment of the gene coding for the large subunit of the form i ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) (rbcl) was amplified by pcr from facultatively lithotrophic aerobic co-oxidizing bacteria, colorless and purple sulfide-oxidizing microbial mats, and genomic dna extracts from tephra and ash deposits from kilauea volcano, for which atmospheric co and hydrogen have been previously documented as important substrates. pcr products from the mats and volcanic site ... | 2004 | 15066819 |
| bacteria are not what they eat: that is why they are so diverse. | | 2000 | 10629168 |
| genomic and functional analyses of the 2-aminophenol catabolic pathway and partial conversion of its substrate into picolinic acid in burkholderia xenovorans lb400. | 2-aminophenol (2-ap) is a toxic nitrogen-containing aromatic pollutant. burkholderia xenovorans lb400 possess an amn gene cluster that encodes the 2-ap catabolic pathway. in this report, the functionality of the 2-aminophenol pathway of b. xenovorans strain lb400 was analyzed. the amnrjbacdfehg cluster located at chromosome 1 encodes the enzymes for the degradation of 2-aminophenol. the absence of haba and habb genes in lb400 genome correlates with its no growth on nitrobenzene. rt-pcr analyses ... | 2013 | 24124510 |
| bacterial quorum sensing in pathogenic relationships. | | 2000 | 10948095 |
| analysis of the dynamics of bacterial communities in the rhizosphere of the chrysanthemum via denaturing gradient gel electrophoresis and substrate utilization patterns | in order to gain a better understanding of the spatial and temporal dynamics of bacterial communities of the rhizosphere of the chrysanthemum, two complementary methods were used: a molecular bacterial community profiling method, i.e., 16s rrna gene-based pcr followed by denaturing gradient gel electrophoresis (dgge), and an agar plate method in which 11 sole-carbon-source utilization tests were used. the dgge patterns showed that the bacterial communities as determined from direct rhizosphere d ... | 1998 | 9835588 |
| bacterial responses and interactions with plants during rhizoremediation. | with the increase in quality of life standards and the awareness of environmental issues, the remediation of polluted sites has become a priority for society. because of the high economic cost of physico-chemical strategies for remediation, the use of biological tools for cleaning-up contaminated sites is a very attractive option. rhizoremediation, the use of rhizospheric microorganisms in the bioremediation of contaminants, is the biotechnological approach that we explore in this minireview. we ... | 2009 | 21255277 |
| genomics of plant-associated microbes. | | 2009 | 21255272 |
| future research trends in the major chemical language of bacteria. | microbiology was revolutionized in the 1990's by the discovery that many different bacterial species coordinate their behavior when they form a group. in fact, bacteria are now considered multicellular organisms capable of communicating and changing behavior in relation to their cell-density; since 1994 this has been called quorum sensing. this group behavior ensures survival and propagation of the community in many natural environments. bacterial intercellular communication is mediated by diffe ... | 2009 | 19794815 |
| structural basis of acyl-homoserine lactone-dependent signaling. | | 2010 | 21125993 |
| structural basis of acyl-homoserine lactone-dependent signaling. | | 2010 | 21125993 |
| plasticity of rhizosphere hydraulic properties as a key for efficient utilization of scarce resources. | it is known that the soil near roots, the so-called rhizosphere, has physical and chemical properties different from those of the bulk soil. rhizosphere properties are the result of several processes: root and soil shrinking/swelling during drying/wetting cycles, soil compaction by root growth, mucilage exuded by root caps, interaction of mucilage with soil particles, mucilage shrinking/swelling and mucilage biodegradation. these processes may lead to variable rhizosphere properties, i.e. the pr ... | 2012 | 23235697 |
| plasticity of rhizosphere hydraulic properties as a key for efficient utilization of scarce resources. | it is known that the soil near roots, the so-called rhizosphere, has physical and chemical properties different from those of the bulk soil. rhizosphere properties are the result of several processes: root and soil shrinking/swelling during drying/wetting cycles, soil compaction by root growth, mucilage exuded by root caps, interaction of mucilage with soil particles, mucilage shrinking/swelling and mucilage biodegradation. these processes may lead to variable rhizosphere properties, i.e. the pr ... | 2012 | 23235697 |
| polysac3db: an annotated data base of 3 dimensional structures of polysaccharides. | polysaccharides are ubiquitously present in the living world. their structural versatility makes them important and interesting components in numerous biological and technological processes ranging from structural stabilization to a variety of immunologically important molecular recognition events. the knowledge of polysaccharide three-dimensional (3d) structure is important in studying carbohydrate-mediated host-pathogen interactions, interactions with other bio-macromolecules, drug design and ... | 2012 | 23151233 |
| rhizobium promotes non-legumes growth and quality in several production steps: towards a biofertilization of edible raw vegetables healthy for humans. | the biofertilization of crops with plant-growth-promoting microorganisms is currently considered as a healthy alternative to chemical fertilization. however, only microorganisms safe for humans can be used as biofertilizers, particularly in vegetables that are raw consumed, in order to avoid sanitary problems derived from the presence of pathogenic bacteria in the final products. in the present work we showed that rhizobium strains colonize the roots of tomato and pepper plants promoting their g ... | 2012 | 22675441 |
| stereochemical insignificance discovered in acinetobacter baumannii quorum sensing. | stereochemistry is a key aspect of molecular recognition for biological systems. as such, receptors and enzymes are often highly stereospecific, only recognizing one stereoisomer of a ligand. recently, the quorum sensing signaling molecules used by the nosocomial opportunistic pathogen, acinetobacter baumannii, were identified, and the primary signaling molecule isolated from this species was n-(3-hydroxydodecanoyl)-l-homoserine lactone. a plethora of bacterial species have been demonstrated to ... | 2012 | 22629354 |
| detection, characterization, and biological effect of quorum-sensing signaling molecules in peanut-nodulating bradyrhizobia. | bacteria of the genus bradyrhizobium are able to establish a symbiotic relationship with peanut (arachis hypogaea) root cells and to fix atmospheric nitrogen by converting it to nitrogenous compounds. quorum sensing (qs) is a cell-cell communication mechanism employed by a variety of bacterial species to coordinate behavior at a community level through regulation of gene expression. the qs process depends on bacterial production of various signaling molecules, among which the n-acylhomoserine la ... | 2012 | 22736981 |
| evolution of and horizontal gene transfer in the endornavirus genus. | the transfer of genetic information between unrelated species is referred to as horizontal gene transfer. previous studies have demonstrated that both retroviral and non-retroviral sequences have been integrated into eukaryotic genomes. recently, we identified many non-retroviral sequences in plant genomes. in this study, we investigated the evolutionary origin and gene transfer of domains present in endornaviruses which are double-stranded rna viruses. using the available sequences for endornav ... | 2013 | 23667703 |
| human clostridium difficile infection: altered mucus production and composition. | the majority of antibiotic-induced diarrhea is caused by clostridium difficile (c. difficile). hospitalizations for c. difficile infection (cdi) have tripled in the last decade, emphasizing the need to better understand how the organism colonizes the intestine and maintain infection. the mucus provides an interface for bacterial-host interactions and changes in intestinal mucus have been linked host health. to assess mucus production and composition in healthy and cdi patients, the main mucins m ... | 2014 | 25552581 |
| human clostridium difficile infection: altered mucus production and composition. | the majority of antibiotic-induced diarrhea is caused by clostridium difficile (c. difficile). hospitalizations for c. difficile infection (cdi) have tripled in the last decade, emphasizing the need to better understand how the organism colonizes the intestine and maintain infection. the mucus provides an interface for bacterial-host interactions and changes in intestinal mucus have been linked host health. to assess mucus production and composition in healthy and cdi patients, the main mucins m ... | 2014 | 25552581 |
| human clostridium difficile infection: inhibition of nhe3 and microbiota profile. | clostridium difficile infection (cdi) is principally responsible for hospital acquired, antibiotic-induced diarrhea and colitis and represents a significant financial burden on our healthcare system. little is known about c. difficile proliferation requirements, and a better understanding of these parameters is critical for development of new therapeutic targets. in cell lines, c. difficile toxin b has been shown to inhibit na(+)/h(+) exchanger 3 (nhe3) and loss of nhe3 in mice results in an alt ... | 2014 | 25552580 |
| human clostridium difficile infection: inhibition of nhe3 and microbiota profile. | clostridium difficile infection (cdi) is principally responsible for hospital acquired, antibiotic-induced diarrhea and colitis and represents a significant financial burden on our healthcare system. little is known about c. difficile proliferation requirements, and a better understanding of these parameters is critical for development of new therapeutic targets. in cell lines, c. difficile toxin b has been shown to inhibit na(+)/h(+) exchanger 3 (nhe3) and loss of nhe3 in mice results in an alt ... | 2014 | 25552580 |
| microbial interactions: ecology in a molecular perspective. | the microorganism-microorganism or microorganism-host interactions are the key strategy to colonize and establish in a variety of different environments. these interactions involve all ecological aspects, including physiochemical changes, metabolite exchange, metabolite conversion, signaling, chemotaxis and genetic exchange resulting in genotype selection. in addition, the establishment in the environment depends on the species diversity, since high functional redundancy in the microbial communi ... | 2016 | 27825606 |
| estimating divergence times and substitution rates in rhizobia. | accurate estimation of divergence times of soil bacteria that form nitrogen-fixing associations with most leguminous plants is challenging because of a limited fossil record and complexities associated with molecular clocks and phylogenetic diversity of root nodule bacteria, collectively called rhizobia. to overcome the lack of fossil record in bacteria, divergence times of host legumes were used to calibrate molecular clocks and perform phylogenetic analyses in rhizobia. the 16s rrna gene and i ... | 2016 | 27168719 |
| detection of hydrogen cyanide from oral anaerobes by cavity ring down spectroscopy. | hydrogen cyanide (hcn) has been recognized as a potential biomarker for non-invasive diagnosis of pseudomonas aeruginosa infection in the lung. however, the oral cavity is a dominant production site for exhaled hcn and this contribution can mask the hcn generated in the lung. it is thus important to understand the sources of hcn production in the oral cavity. by screening of oral anaerobes for hcn production, we observed that the genus of porphyromonas, prevotella and fusobacterium generated low ... | 2016 | 26940198 |
| analysis of biolog gn substrate utilization patterns by microbial communities. | biolog gn plates are increasingly used to characterize microbial communities by determining the ability of the communities to oxidize various carbon sources. studies were done to determine whether the biolog gn plate assay accurately reflects the catabolic potential of the inoculum used. to gain insight into which populations of microbial communities contribute to the biolog patterns, denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis (tgge) were used to assess ... | 1998 | 16349535 |
| response of atmospheric methane consumption by maine forest soils to exogenous aluminum salts. | atmospheric methane consumption by maine forest soils was inhibited by additions of environmentally relevant levels of aluminum. aluminum chloride was more inhibitory than nitrate or sulfate salts, but its effect was comparable to that of a chelated form of aluminum. inhibition could be explained in part by the lower soil ph values which resulted from aluminum addition. however, significantly greater inhibition by aluminum than by mineral acids at equivalent soil ph values indicated that inhibit ... | 2000 | 10966375 |
| horizontally transferred genes in plant-parasitic nematodes: a high-throughput genomic approach. | published accounts of horizontally acquired genes in plant-parasitic nematodes have not been the result of a specific search for gene transfer per se, but rather have emerged from characterization of individual genes. we present a method for a high-throughput genome screen for horizontally acquired genes, illustrated using expressed sequence tag (est) data from three species of root-knot nematode, meloidogyne species. | 2003 | 12801413 |
| rhizospheric pseudomonads: friends or foes? | of the different groups of soil microorganisms, pseudomonads are one of the important class, playing various roles in the plants growth and development. although they have been reported to inflict both beneficial and harmful effect on plants, they act through various mechanisms. among the different mechanisms, cyanogenesis is one of the important factors used by pseudomonads to cause positive and less studied negative effects in the rhizosphere. by employing a bioassay driven approach, we dissec ... | 2008 | 19704457 |
| an ancient p-loop gtpase in rice is regulated by a higher plant-specific regulatory protein. | ychf is a subfamily of the obg family in the trafac class of p-loop gtpases. the wide distribution of ychf homologues in both eukarya and bacteria suggests that they are descendents of an ancient protein, yet their physiological roles remain unclear. using the osychf1-osgap1 pair from rice as the prototype, we provide evidence for the regulation of gtpase/atpase activities and rna binding capacity of a plant ychf (osychf1) by its regulatory protein (osgap1). the effects of osgap1 on the subcellu ... | 2010 | 20876569 |
| malonyl-coa synthetase, encoded by acyl activating enzyme13, is essential for growth and development of arabidopsis. | malonyl-coa is the precursor for fatty acid synthesis and elongation. it is also one of the building blocks for the biosynthesis of some phytoalexins, flavonoids, and many malonylated compounds. in plants as well as in animals, malonyl-coa is almost exclusively derived from acetyl-coa by acetyl-coa carboxylase (ec 6.4.1.2). however, previous studies have suggested that malonyl-coa may also be made directly from malonic acid by malonyl-coa synthetase (ec 6.2.1.14). here, we report the cloning of ... | 2011 | 21642549 |
| calcium causes multimerization of the large adhesin lapf and modulates biofilm formation by pseudomonas putida. | lapf is a large secreted protein involved in microcolony formation and biofilm maturation in pseudomonas putida. its c-terminal domain shows the characteristics of proteins secreted through a type i secretion system and includes a predicted calcium binding motif. we provide experimental evidence of specific binding of ca(2+) to the purified c-terminal domain of lapf (clapf). calcium promotes the formation of large aggregates, which disappear in the presence of the calcium chelator egta. immunolo ... | 2012 | 23042991 |
| regulatable vectors for environmental gene expression in alphaproteobacteria. | two expression vectors utilizing the inducible taurine promoter (tauap) were developed. plasmid plmb51 is a stable low-copy vector enabling expression in the environment and in planta. the higher copy number plmb509 enables bd restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins. | 2012 | 22820336 |
| extracytoplasmic function (ecf) sigma factor σf is involved in caulobacter crescentus response to heavy metal stress. | the α-proteobacterium caulobacter crescentus inhabits low-nutrient environments and can tolerate certain levels of heavy metals in these sites. it has been reported that c. crescentus responds to exposure to various heavy metals by altering the expression of a large number of genes. | 2012 | 22985357 |
| modifications of xanthomonas axonopodis pv. citri lipopolysaccharide affect the basal response and the virulence process during citrus canker. | xanthomonas axonopodis pv. citri (xac) is the phytopathogen responsible for citrus canker, one of the most devastating citrus diseases in the world. a broad range of pathogens is recognized by plants through so-called pathogen-associated molecular patterns (pamps), which are highly conserved fragments of pathogenic molecules. in plant pathogenic bacteria, lipopolisaccharyde (lps) is considered a virulence factor and it is being recognized as a pamp. the study of the participation of xac lps in c ... | 2012 | 22792211 |
| regulation and evolution of malonate and propionate catabolism in proteobacteria. | bacteria catabolize malonate via two pathways, encoded by the mdc and mat genes. in various bacteria, transcription of these genes is controlled by the gntr family transcription factors (tfs) matr/mdcy and/or the lysr family transcription factor mdcr. propionate is metabolized via the methylcitrate pathway, comprising enzymes encoded by the prp and acn genes. prpr, the fis family sigma 54-dependent transcription factor, is known to be a transcriptional activator of the prp genes. here, we report ... | 2012 | 22505679 |
| mutation in the pssa gene involved in exopolysaccharide synthesis leads to several physiological and symbiotic defects in rhizobium leguminosarum bv. trifolii. | the symbiotic nitrogen-fixing bacterium rhizobium leguminosarum bv. trifolii 24.2 secretes large amounts of acidic exopolysaccharide (eps), which plays a crucial role in establishment of effective symbiosis with clover. the biosynthesis of this heteropolymer is conducted by a multi-enzymatic complex located in the bacterial inner membrane. pssa protein, responsible for the addition of glucose-1-phosphate to a polyprenyl phosphate carrier, is involved in the first step of eps synthesis. in this w ... | 2013 | 24317432 |
| celr, an ortholog of the diguanylate cyclase pled of caulobacter, regulates cellulose synthesis in agrobacterium tumefaciens. | cellulose fibrils play a role in attachment of agrobacterium tumefaciens to its plant host. while the genes for cellulose biosynthesis in the bacterium have been identified, little is known concerning the regulation of the process. the signal molecule cyclic di-gmp (c-di-gmp) has been linked to the regulation of exopolysaccharide biosynthesis in many bacterial species, including a. tumefaciens. in this study, we identified two putative diguanylate cyclase genes, celr (atu1297) and atu1060, that ... | 2013 | 24038703 |
| salmonella utilizes d-glucosaminate via a mannose family phosphotransferase system permease and associated enzymes. | salmonella enterica is a globally significant bacterial food-borne pathogen that utilizes a variety of carbon sources. we report here that salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) uses d-glucosaminate (2-amino-2-deoxy-d-gluconic acid) as a carbon and nitrogen source via a previously uncharacterized mannose family phosphotransferase system (pts) permease, and we designate the genes encoding the permease dgaabcd (d-glucosaminate pts permease components eiia, eiib, e ... | 2013 | 23836865 |
| luxr- and luxi-type quorum-sensing circuits are prevalent in members of the populus deltoides microbiome. | we are interested in the root microbiome of the fast-growing eastern cottonwood tree, populus deltoides. there is a large bank of bacterial isolates from p. deltoides, and there are 44 draft genomes of bacterial endophyte and rhizosphere isolates. as a first step in efforts to understand the roles of bacterial communication and plant-bacterial signaling in p. deltoides, we focused on the prevalence of acyl-homoserine lactone (ahl) quorum-sensing-signal production and reception in members of the ... | 2013 | 23851092 |
| root exudation of phytochemicals in arabidopsis follows specific patterns that are developmentally programmed and correlate with soil microbial functions. | plant roots constantly secrete compounds into the soil to interact with neighboring organisms presumably to gain certain functional advantages at different stages of development. accordingly, it has been hypothesized that the phytochemical composition present in the root exudates changes over the course of the lifespan of a plant. here, root exudates of in vitro grown arabidopsis plants were collected at different developmental stages and analyzed using gc-ms. principle component analysis reveal ... | 2013 | 23383346 |
| structure and biological roles of sinorhizobium fredii hh103 exopolysaccharide. | here we report that the structure of the sinorhizobium fredii hh103 exopolysaccharide (eps) is composed of glucose, galactose, glucuronic acid, pyruvic acid, in the ratios 5∶2∶2∶1 and is partially acetylated. a s. fredii hh103 exoa mutant (svq530), unable to produce eps, not only forms nitrogen fixing nodules with soybean but also shows increased competitive capacity for nodule occupancy. mutant svq530 is, however, less competitive to nodulate vigna unguiculata. biofilm formation was reduced in ... | 2014 | 25521500 |
| identification of pseudomonas fluorescens chemotaxis sensory proteins for malate, succinate, and fumarate, and their involvement in root colonization. | pseudomonas fluorescens pf0-1 exhibited chemotactic responses to l-malate, succinate, and fumarate. we constructed a plasmid library of 37 methyl-accepting chemotaxis protein (mcp) genes of p. fluorescens pf0-1. to identify a mcp for l-malate, the plasmid library was screened using the pa2652 mutant of pseudomonas aeruginosa pao1, a mutant defective in chemotaxis to l-malate. the introduction of pfl01_0728 and pfl01_3768 genes restored the ability of the pa2652 mutant to respond to l-malate. the ... | 2014 | 25491753 |
| examination of prokaryotic multipartite genome evolution through experimental genome reduction. | many bacteria carry two or more chromosome-like replicons. this occurs in pathogens such as vibrio cholerea and brucella abortis as well as in many n2-fixing plant symbionts including all isolates of the alfalfa root-nodule bacteria sinorhizobium meliloti. understanding the evolution and role of this multipartite genome organization will provide significant insight into these important organisms; yet this knowledge remains incomplete, in part, because technical challenges of large-scale genome m ... | 2014 | 25340565 |
| bacterial metabolism of methylated amines and identification of novel methylotrophs in movile cave. | movile cave, romania, is an unusual underground ecosystem that has been sealed off from the outside world for several million years and is sustained by non-phototrophic carbon fixation. methane and sulfur-oxidising bacteria are the main primary producers, supporting a complex food web that includes bacteria, fungi and cave-adapted invertebrates. a range of methylotrophic bacteria in movile cave grow on one-carbon compounds including methylated amines, which are produced via decomposition of orga ... | 2014 | 25050523 |
| bacterial metabolism of methylated amines and identification of novel methylotrophs in movile cave. | movile cave, romania, is an unusual underground ecosystem that has been sealed off from the outside world for several million years and is sustained by non-phototrophic carbon fixation. methane and sulfur-oxidising bacteria are the main primary producers, supporting a complex food web that includes bacteria, fungi and cave-adapted invertebrates. a range of methylotrophic bacteria in movile cave grow on one-carbon compounds including methylated amines, which are produced via decomposition of orga ... | 2014 | 25050523 |
| sinorhizobium meliloti chemoreceptor mcpu mediates chemotaxis toward host plant exudates through direct proline sensing. | bacterial chemotaxis is an important attribute that aids in establishing symbiosis between rhizobia and their legume hosts. plant roots and seeds exude a spectrum of molecules into the soil to attract their bacterial symbionts. the alfalfa symbiont sinorhizobium meliloti possesses eight chemoreceptors to sense its environment and mediate chemotaxis toward its host. the methyl accepting chemotaxis protein mcpu is one of the more abundant s. meliloti chemoreceptors and an important sensor for the ... | 2014 | 24657863 |
| biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. | current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. the exploitation of beneficial microbes as a biofertilizer has become paramount importance in agriculture sector for their potential role in food safety and sustainable crop production. the eco-friendly approaches inspire a wide range of application of plant growth promoting rhizobacteria (pgprs), endo- and ectomycorrhizal fungi, cyanobac ... | 2014 | 24885352 |
| characterization and fungal inhibition activity of siderophore from wheat rhizosphere associated acinetobacter calcoaceticus strain hirfa32. | acinetobacter calcoaceticus hirfa32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without feso4 at 28 °c and 24 h of incubation. hplc purified siderophore appeared as pale yellow crystals with molecular weight [m(+1)] m/z 347.18 estimated by lcms. the structure elucidated by (1)h nmr, (13)c nmr, hmqc, hmbc, noesy and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid w ... | 2014 | 24891739 |
| identification of bradyrhizobium elkanii genes involved in incompatibility with soybean plants carrying the rj4 allele. | symbioses between leguminous plants and soil bacteria known as rhizobia are of great importance to agricultural production and nitrogen cycling. while these mutualistic symbioses can involve a wide range of rhizobia, some legumes exhibit incompatibility with specific strains, resulting in ineffective nodulation. the formation of nodules in soybean plants (glycine max) is controlled by several host genes, which are referred to as rj genes. the soybean cultivar barc2 carries the rj4 gene, which re ... | 2015 | 26187957 |
| metabolomics reveals differences of metal toxicity in cultures of pseudomonas pseudoalcaligenes kf707 grown on different carbon sources. | co-contamination of metals and organic pollutants is a global problem as metals interfere with the metabolism of complex organics by bacteria. based on a prior observation that metal tolerance was altered by the sole carbon source being used for growth, we sought to understand how metal toxicity specifically affects bacteria using an organic pollutant as their sole carbon source. to this end metabolomics was used to compare cultures of pseudomonas pseudoalcaligenes kf707 grown on either biphenyl ... | 2015 | 26347721 |
| identification of the mcpa and mcpm genes, encoding methyl-accepting proteins involved in amino acid and l-malate chemotaxis, and involvement of mcpm-mediated chemotaxis in plant infection by ralstonia pseudosolanacearum (formerly ralstonia solanacearum phylotypes i and iii). | sequence analysis has revealed the presence of 22 putative methyl-accepting chemotaxis protein (mcp) genes in the ralstonia pseudosolanacearum gmi1000 genome. pcr analysis and dna sequencing showed that the highly motile r. pseudosolanacearum strain ps29 possesses homologs of all 22 r. pseudosolanacearum gmi1000 mcp genes. we constructed a complete collection of single mcp gene deletion mutants of r. pseudosolanacearum ps29 by unmarked gene deletion. screening of the mutant collection revealed t ... | 2015 | 26276117 |