| a role for dystroglycan in epithelial polarization: loss of function in breast tumor cells. | receptors mediating cell-basement membrane interactions are potent regulators of epithelial architecture and function, and alterations in signals from the basement membrane are implicated in the aberrant behavior of carcinoma cells. in this study, we have investigated the role of the basement membrane receptor dystroglycan (dg) in mammary epithelial cell function, and the significance of loss of dg function in breast tumor cell lines. nonmalignant mammary epithelial cells express a functional dg ... | 2002 | 12460932 |
| goiter prevalence, urinary iodine excretion, thyroid function and anti-thyroid function and anti-thyroid antibodies after 12 years of salt iodization in shahriar, iran. | in a previous study in 1983, goiter was found to be endemic in shahriar, iran. iodized salt has been distributed in the region for the past 12 years, and the present study was performed to examine the effect of iodide supplementation on indicators of iodine deficiency (idd) and thyroid antibodies. | 2002 | 12463103 |
| the relation between serum ferritin and goiter, urinary iodine and thyroid hormone concentration. | many children are at high risk of both goiter and iron deficiency in iran. because iron deficiency may impair the efficacy of iodine supplementation, the aim of this study was to determine the relation between serum ferritin and goiter, urinary iodine, and thyroid hormones following iodized salt supplementation. | 2002 | 12463104 |
| [renal cell carcinoma before the age of 40: prognostic factors]. | to study the prognostic value of tumour diameter, tnm stage, führman's nuclear grade and cd44 adhesion molecule expression in renal cell carcinoma (rcc) before the age of 40 years. | 2002 | 12463113 |
| synthesis of a highly fluorescent beta-diketone-europium chelate and its utility in time-resolved fluoroimmunoassay of serum total thyroxine. | a new highly fluorescent beta-diketone-europium chelate was synthesized and employed as a tracer to develop a time-resolved fluoroimmunoassay (trfia) for detection of serum total thyroxine (t4). the tetradentate beta-diketone chelator, 1,10-bis(thiophene-2'-yl)-4,4,5,5,6,6,7,7-octafluorodecane-1,3,8,10-tetraone (btot), was structurally composed of two units of thenoyltrifluoroacetone (tta) derivatives but expressed fluorescence that was greatly enhanced, as compared to the original tta molecules ... | 2002 | 12463376 |
| euthyroid sick syndrome in acute ischemic syndromes. | the purposes of this study were to assess the occurrence of euthyroid sick syndrome in patients with acute myocardial infarction (ami) or unstable angina (ua), and the relationship with beta-blocker or thrombolytic therapy. plasma triiodothyronine (t3), reverse t3 (rt3), free t3 (ft3), thyroxine (t4), free t4 (ft4), thyroid-stimulating hormone (tsh), thyroxine-binding globulin (tbg), and albumin (alb) levels were determined in 95 patients (59 males, 36 females, aged 58.4+/-9) with ami and 19 pat ... | 2002 | 12463624 |
| [a case of thymic carcinoma responded to intra-arterial chemotherapy combined with radiation therapy]. | a 70-year-old male complained of pain in the left neck. a tumor of about 8 cm in diameter was detected by contrast ct in the region extending from the anterior to upper mediastinum. for definitive diagnosis, biopsy was performed under ct guidance, and the patient was diagnosed as having anaplastic carcinoma in the thymus (t4, n1, m0, stage ivb). since surgical treatment was judged to be impossible because of infiltration into large blood vessels, the patient underwent chemotherapy by intra-arter ... | 2002 | 12465397 |
| [postoperative adjuvant immunochemotherapy using lentinan for advanced gastric carcinoma patients with metastasis in the regional lymph nodes and serosal invasion]. | six patients with gastric cancer, stage iiia to iv, received intraabdominal cisplatin (cddp) at laporotomy. this was followed by postoperative intravenous infusion of mitomycin c (mmc), cddp or fluorouracil (5-fu). when these patients recovered to the extent that permitted oral medication, an immunochemotherapeutic regimen containing either oral uft (uracil and tegafur) or 5'-deoxy-5-fluorouridine (5'-dfur), plus intravenous lentinan (lnt) was administered for a period ranging from one and a hal ... | 2002 | 12465402 |
| isolation and characterization of t4 bacteriophage gp17 terminase, a large subunit multimer with enhanced atpase activity. | phage t4 terminase is a two-subunit enzyme that binds to the prohead portal protein and cuts and packages a headful of concatameric dna. to characterize the t4 terminase large subunit, gp17 (70 kda), gene 17 was cloned and expressed as a chitin-binding fusion protein. following cleavage and release of gp17 from chitin, two additional column steps completed purification. the purification yielded (i) homogeneous soluble gp17 highly active in in vitro dna packaging ( approximately 10% efficiency, > ... | 2003 | 12466275 |
| circle ligation of in vitro assembled chromatin indicates a highly flexible structure. | evidence is provided that some condensed linker histone-containing chromatin structures are highly flexible in solutions containing 2 mm mg2+. chromatin assembled in vitro +/- histone h5 on a 6.3 kb linear dna fragment in 90 mm nacl using the polyglutamic acid method sedimented fairly homogeneously. the h5-containing sample had s(20, w) values that were 58-69% greater than the sample lacking h5. chromatin assembled on linear puc19 plasmid dna was treated with t4 dna ligase in solutions containin ... | 2002 | 12466533 |
| the tail lysozyme complex of bacteriophage t4. | the tail baseplate of bacteriophage t4 contains a structurally essential, three-domain protein encoded by gene 5 in which the middle domain possesses lysozyme activity. the gene 5 product (gp5) undergoes post-translational cleavage, allowing the resultant n-terminal domain (gp5*) to assemble into the baseplate as a trimer. the lysozyme activity of the undissociated cleaved gp5 is inhibited until infection has been initiated, when the c-terminal portion of the molecule is detached and the rest of ... | 2003 | 12467643 |
| sucrose feeding effects inhibition of gamma-glutamyltranspeptidase in the liver of the rat: possible mediation by thyroid hormone. | the effect of sucrose on fischer 344 rat liver gamma-glutamyltranspeptidase (gammagt) was studied: in adults fed sucrose for 3 weeks; and rats exposed to sucrose from the 18th day of gestation to the 40th day after birth. rats fed regular rodent chow served as controls. sucrose caused mild lipemia; and in the liver an increase in size and fat build-up without damage. in adult sucrose-fed rats, compared to controls, plasma glucose levels were increased: 1.12-, 1.40- and 1.13-fold after 24, 48h an ... | 2003 | 12467647 |
| comparison of clinical and surgical-pathologic staging of the patients with non-small cell lung carcinoma. | clinical staging of non-small cell lung cancer helps to determine the extent of disease and separate patients with potentially resectable disease from those that are unresectable. since, clinical staging is based on radiologic and bronchoscopic findings, overstaging or understaging may occur comparing to the final surgical-pathologic evaluation. we aimed to analyze preoperative and postoperative stagings in order to evaluate stage migrations and our surgical strategy for marginally resectable pa ... | 2002 | 12467827 |
| paraparesis in a black man brought on by ossification of the ligamentum flavum: case report and review of the literature. | we present the second case of paraparesis secondary to ossification of the ligamentum flavum at the midthoracic region in a black man. ossification of the ligamentum flavum is frequently described in the japanese population where the presentation is often in the lower thoracic region. the patient is a 37-year-old black man who, over the 6 months before admission, noticed progressive paraparesis. ct myelogram revealed severe thoracic stenosis by an ossified ligamentum flavum from t4 to t7 with mo ... | 2002 | 12468987 |
| in vitro effects of thyroid hormones on ectonucleotidase activities in synaptosomes from hippocampus of rats. | 1. studies have shown that adenosine transport and adenosine a1 receptors in rat brain are subjected to regulation by thyroid hormone levels. since the ectonucleotidase pathway is an important source of adenosine extracellular, in the present study the in vitro action of t3 and t4 hormones on ectonucleotidase activities in hippocampal synaptosomes was evaluated. 2. t3 (triiodo-l-thyronine) significantly inhibited, in an uncompetitive manner, the atp and adp hydrolysis promoted by atp diphosphohy ... | 2002 | 12469875 |
| treatment of hyperfunctioning thyroid nodules by percutaneous ethanol injection. | background: autonomous thyroid nodules can be treated by a variety of methods. we assessed the efficacy of percutaneous ethanol injection in treating autonomous thyroid nodules. methods: 35 patients diagnosed by technetium-99 scanning with hyperfunctioning nodules and suppressed sensitive tsh (stsh) were given sterile ethanol injections under ultrasound guidance. 29 patients had clinical and biochemical hyperthyroidism. the other 6 had sub-clinical hyperthyroidism with suppressed stsh levels (<0 ... | 2002 | 12470301 |
| type iv collagen, type iv collagenase activity and ability of cell proliferation in human thyroid tumours. | the processes of malignant tumour invasion and metastasis are known to include the destruction of cell stroma and vascular basement membrane. it has been suggested that type iv collagenase degrades type iv collagen, a main component of the basement membrane. | 2002 | 12471003 |
| o-galnac incorporation into a cluster acceptor site of three consecutive threonines. distinct specificity of galnac-transferase isoforms. | o-glycosylation of three consecutive thr residues in a fluorescein-conjugated peptide ptttplk - which mimics a portion of mucin 2 - by four isozymes of udp-n-acetylgalactosaminyltransferases (pp-galnac-t1, t2, t3, or t4) was investigated. partially glycosylated versions of this peptide, pt*ttplk, pttt*plk, pt*tt*plk, ptt*t*plk, pt* degrees ttplk, and pttt* degrees plk (*, n-acetylgalactosamine; degrees, galactose), were also tested. the products were separated by rp-hplc and characterized by mal ... | 2002 | 12473113 |
| excision of the levator muscles with external sphincter preservation in the treatment of selected low t4 rectal cancers. | the existence of an anatomic and functional separation between the puborectalis muscle and external anal sphincter permits the performance of an abdominoendoanal excision, instead of an abdominoperineal excision, of the rectum and levator muscles, with preservation of a functioning external sphincter, in selected patients with very low rectal cancer and limited infiltration of the levator muscles. | 2002 | 12473899 |
| insight into the physiological actions of thyroid hormone receptors from genetically modified mice. | thyroid hormones exert a range of developmental and physiological actions in all vertebrates. serum concentrations of l-thyroxine (t4) and 3,5,3 -l-triiodothyronine (t3) are maintained by a negative feedback loop involving t3-inhibition of hypothalamic thyrotrophin releasing hormone (trh) and pituitary thyroid stimulating hormone (tsh) secretion, and by tissue specific and hormone-regulated expression of the three iodothyronine deiodinase enzymes that activate or metabolise thyroid hormones. t3 ... | 2002 | 12475367 |
| is the low tri-iodothyronine state a crucial factor in determining the outcome of coronary artery bypass patients? evidence from a clinical pilot study. | the cardiovascular system is an important target for thyroid hormones. the present study evaluates the changes affecting thyroid hormone metabolism during and 6 days after coronary artery bypass and their relationship with the post-operative outcome of the patients. thirty-three patients were enrolled in the study; their thyroid hormone profiles were determined at 13 sampling points during surgery and for 6 days afterwards. serum total tri-iodothyronine (t3) and free t3 (ft3) concentrations decr ... | 2002 | 12475369 |
| influence of maternal hyperthyroidism in the rat on the expression of neuronal and astrocytic cytoskeletal proteins in fetal brain. | maternal hypothyroidism during pregnancy impairs brain function in human and rat offspring, but little is known regarding the influence of maternal hyperthyroidism on neurodevelopment. we have previously shown that the expression of neuronal and glial differentiation markers in fetal brain is compromised in hypothyroid rat dam pregnancies and have now therefore extended this investigation to hyperthyroid rat dams. study groups comprised partially thyroidectomised dams, implanted with osmotic pum ... | 2002 | 12475371 |
| continuous age-dependent reference ranges for thyroid hormones in neonates, infants, children and adolescents established using the advia centaur analyzer. | in neonates, infants, children and adolescents serum concentrations of the thyroid hormones show a clear age dependency. currently, age-dependent thyroid hormone reference ranges for the new advia centaur immunoanalyzer are not available. thyroid-stimulating hormone (tsh), free thyroxine (ft4), total thyroxine (t4), free triiodothyronine (ft3) and total triiodothyronine (t3) were determined in the sera of 460 healthy children aged 0 daysto 18 years from an inland (n=308) and a seaside city (n=15 ... | 2002 | 12476946 |
| the coenzyme thiamine pyrophosphate inhibits the self-splicing of the group i intron. | effects of the coenzyme thiamine pyrophosphate and its analogs on the inhibition of self-splicing of primary transcripts of the phage t4 thymidylate synthase gene (td) were investigated. of all compounds tested, the coenzyme thiamine pyrophosphate was the most potent inhibitor and the order of inhibitory efficiency for compounds tested was as follows: thiamine pyrophosphate>thiamine monophosphate>thiamine>thiochrome. increasing guanosine concentration overcame the suppression of self-splicing by ... | 2003 | 12479866 |
| chemical activation of cardiac receptors affects activity of superficial and deeper t3-t4 spinal neurons in rats. | the purposes of this study were to examine responses of superficial (depth <300 microm) and deeper thoracic spinal neurons to chemical stimulation of cardiac afferents and effects of descending influences on these neurons. extracellular potentials of single t(3)-t(4) neurons were recorded in pentobarbital anesthetized, paralyzed and ventilated male rats. a catheter was placed in the pericardial sac to administer 0.2 ml of a mixture of algogenic chemicals that contained adenosine (10(-3) m), brad ... | 2003 | 12480160 |
| effect of nutritional stress on the hypothalamo-pituitary-gonadal axis in the growing male rat. | nutritional dwarfing (nd) consists of a decrease in weight and height gain and delayed onset of puberty. the aim of the present investigation was to study the modifications induced in male rats by the nutritional stress of a mere 20% reduction in food intake which, however, started immediately after weaning. | 2002 | 12481155 |
| total synthesis of amphidinolide t4. | | 2002 | 12481352 |
| circulating il-8 and il-10 in euthyroid sick syndromes following bone marrow transplantation. | it is generally agreed that euthyroid sick syndromes (ess) are associated with an increased production of cytokines. however, there has been scarce data on the relationship thyroid hormone changes and cytokines among the patients undergoing bone marrow transplantation (bmt). because interleukin-8 (il-8) has been identified as a potent proinflammatory and interleukin-10 (il-10) as an antiinflammatory cytokine, we studied the relation between thyroid hormone parameters and these cytokines followin ... | 2002 | 12482997 |
| 'in vitro' binding of propofol to serum lipoproteins in thyroid dysfunction. | to determine a regression relationship between the unbound fraction (fu percent) of propofol, a highly lipophilic intravenous anaesthetic agent, and demographic and biochemical variables in a thyroid dysfunction population. | 2002 | 12483454 |
| [chemoradiotherapy using platinum analogs/5-fu for advanced esophageal cancer]. | we evaluated the efficacy of concurrent chemoradiotherapy (crt) using cisplatin/nedaplatin and 5-fu for advanced esophageal cancer. thirteen patients with locally advanced esophageal cancer (t4 cases) and 3 with recurrence of esophageal cancer were treated with radiotherapy (40-70 gy) and 5-fu combined with cisplatin/nedaplatin concurrently. t4 patients who obtained down-staging by crt also underwent esophagectomy. a complete response was obtained in one case, partial response in 8 cases, and no ... | 2002 | 12484039 |
| effects of methimazole pretreatment on cerulein induced acute pancreatitis in rats. | many interrelationships exist between the thyroid gland and the gastrointestinal tract. several past and recent studies have shown that the thyroid gland profoundly influences the structure and function of the exocrine pancreas in the rat. in the present study we investigated the effect of methimazole (metz), an antithyroid drug, on cerulein induced acute pancreatitis (ap) in rats. | 2002 | 12484556 |
| [prognostic factors in patients with primary non-hodgkin's lymphoma of the tonsil]. | to investigate the prognostic value of the size of primary tumor (t staging) and international prognostic index (ipi) for patients with non-hodgkin's lymphoma (nhl) of the tonsil, and to recommend the treatment strategy for early stage patients. | 2002 | 12485504 |
| making appdna using t4 dna ligase. | 5('),5(')-adenylyl pyrophosphoryl dna (appdna) contains a high-energy pyrophosphate linkage and can be exploited as an activated dna substrate to derive new dna enzymes for carrying out various dna modification reactions. for this reason, enzymatic synthesis of appdna is highly desirable. appdna is a known intermediate in dna ligase mediated dna ligation reactions, but rarely accumulates under normal reaction conditions. here we report that t4 dna ligase can quantitatively convert 5(')-phosphory ... | 2002 | 12485593 |
| [myxedema coma as a rare differential diagnosis of severe consciousness disturbance]. | myxedema coma is a rare and life-threatening complication of untreated hypothyroidism. therefore, it must be part of the differential diagnosis in comatose patients. we report one patient who presented with co(2) narcosis,hypothermia, bradycardia,hyporeflexia, tetraparesis, ascitis, pleural effusions, and heart insufficiency. examination of the csf, cranial ct, mri, and mr angiography were normal. in suspicion of myxedema coma,the patient was treated with high dose l-thyroxine and hydrocortisone ... | 2002 | 12486569 |
| the invasive phenotype in hmt-3522 cells requires increased egf receptor signaling through both pi 3-kinase and erk 1,2 pathways. | we studied the invasion of hmt-3522 breast epithelial cells in response to epidermal growth factor (egf), and the associated signaling pathways. hmt-3522 t4-2 cells were shown to invade matrigel-coated transwell membranes in response to egf while hmt-3522 s-1 cells failed to invade when treated with egf. studies utilizing specific molecular inhibitors showed the importance of beta1 integrin, phosphatidylinositol 3 kinase (pi 3-kinase), p38, extracellular regulated kinase 1, 2 (erk 1,2) map kinas ... | 2002 | 12487410 |
| a helix initiation signal in t4 lysozyme identified by polyalanine mutagenesis. | to better understand the relation between sequence and structure, and in an attempt to simplify the protein folding problem, a series of alanine substitutions was introduced into bacteriophage t4 lysozyme. in contrast to previous studies in this system, which were restricted to single alpha-helices, the present analysis included a helix-turn-helix region, a loop-helix region, and two alpha-helices that were well separated in the three-dimensional structure. it was shown previously that t4 lysozy ... | 2002 | 12487988 |
| wall effects on dna stretch and relaxation. | hydrodynamic wall effects are treated with an image or reflection method. this method uses a mirror image of the molecule, with the opposite velocity, to satisfy the non-slip boundary condition of zero velocity of the liquid at the wall. molecules moving inside a slit require an infinite series of images, or reflections from both walls, whose effects converge slower for thinner slits. it is shown that, with the same external field, wall effects increase the electrophoretic stretch of dna, more s ... | 2002 | 12488019 |
| use of t4 lysozyme charge mutants to examine electrophoretic models. | the electrophoretic mobility of a macro-ion is affected in a complex manner by a variety of forces that arise from the applied field. coupling of the macro-ion and small-ion flows gives rise to non-conserved forces that are greater than those expected from ordinary hydrodynamic considerations. it is difficult to separate the steady-state hydrodynamic and electrodynamic contributions to the macro-ion mobility. membrane-confined electrophoresis (mce), a free solution technique, provides an experim ... | 2002 | 12488029 |
| statistical analysis of hormonal influences on arousal measures in ovariectomized female mice. | in a previous article (j. frohlich, m. morgan, s. ogawa, l. burton, and d. pfaff, 2001, horm. behav. 39, 39-47) an experiment to explore the structure of behavioral arousal in female mice was described. the present study extends this, to investigate the roles of thyroid hormone and estradiol in altering the statistical structure of arousal measures. each of four groups of ovariectomized female mice was administered either thyroxine (t4), estradiol benzoate (eb), both (t4 + eb), or neither (contr ... | 2002 | 12488108 |
| tc-99m-tetrofosmin thyroid scan in patients with low i-131 thyroid uptake. | low 24-hour thyroid uptake of i-131 (i-131 uptake) is a common finding that influences evaluation of the thyroid gland. | 2002 | 12489572 |
| necrosis of nasal and airway epithelium in rats inhaling vapors of artificial butter flavoring. | as the result of a high prevalence of fixed airways obstruction in workers at a microwave popcorn manufacturing plant, we examined the hypothesis that vapors of butter flavoring used in the manufacture of microwave popcorn and other foods can produce airway injury in rats. rats were exposed to vapors liberated from heated butter flavoring. rats were exposed for 6 h by inhalation and were necropsied 1 day after exposure. the exposure was found by gc-ms analysis to be a complex mixture of various ... | 2002 | 12490137 |
| ret proto-oncogene mutations affecting codon 790/791: a mild form of multiple endocrine neoplasia type 2a syndrome? | in patients with multiple endocrine neoplasia type 2a syndrome, prophylactic thyroidectomy is generally recommended at the age of 5 to 6 years. whether this recommendation is justified for exon 13 mutations is unknown. | 2002 | 12490841 |
| prolactin and thyroid hormones in patients with systemic sclerosis: correlations with disease manifestations and activity. | to determine serum levels of prolactin (prl) and thyroid hormones and to investigate the correlation between these hormones and different disease manifestations in patients with systemic sclerosis (ssc). | 2002 | 12491136 |
| diagnosis of neck recurrences in patients with differentiated thyroid carcinoma. | the follow-up of patients with differentiated thyroid carcinoma (dtc) is traditionally carried out with (131)i whole body scan ((131)i wbs) and serum thyroglobulin (tg) measurement. neck ultrasonography (us) is also used. | 2003 | 12491509 |
| pre- and postprandial changes in plasma hormone and metabolite levels and hepatic deiodinase activities in meal-fed broiler chickens. | the present study aimed to study the effects of food deprivation and subsequent postprandial changes in plasma somatotrophic and thyrotrophic hormone levels and focused on the inter-relationships between these hormonal axes and representative metabolites of the intermediary metabolism of meal-fed broiler chickens. male broiler chickens (2 weeks old) were fed a meal of 40-45 g/bird per d for two consecutive weeks (food-restricted (fr) treatment). the daily allowance was consumed in about 30 min. ... | 2002 | 12493086 |
| using t4 dna polymerase to generate clonable pcr products. | | 2002 | 12494644 |
| mutational and functional analysis of a segment of the sigma family bacteriophage t4 late promoter recognition protein gp55. | bacteriophage t4 late promoters, which consist of a simple 8-base pair tata box, are recognized by the gene 55 protein (gp55), a small, highly diverged member of the sigma family proteins that replaces sigma(70) during the final phase of the t4 multiplication cycle. a 16-amino acid segment of gp55 that is proposed to be homologous to the sigma(70) region 2.2 has been subjected to alanine scanning and other mutagenesis. the corresponding proteins have been examined in vitro for binding to escheri ... | 2003 | 12496274 |
| distribution of vascular endothelial growth factor (vegf) in prostate disease. | vascular endothelial growth factor (vegf) is a heparin-binding polypeptide growth factor. it is a potent mitogen for endothelial cells. immunohistochemical localisation of vegf was performed on 25 moderate to poorly differentiated stage t4 m+ prostate cancer specimens and 30 benign prostatic hyperplasia (bph) specimens. a positive result was indicated by area staining >25% and +2 or +3 staining intensity. positive epithelial staining was observed in 50% of bph specimens and 56% of cancer specime ... | 2002 | 12497000 |
| the role of pretreatment serum albumin to predict pathological stage and recurrence among radical prostatectomy cases. | serum albumin level has been implicated as a prognostic factor in various cancers, however it has not been studied in early stage prostate cancer. we examine the clinical prognostic value of the readily available pretreatment albumin level as a marker for determining staging and prognosis for men undergoing radical prostatectomy. a retrospective review was performed on 354 patients who underwent a radical prostatectomy at a tertiary facility from 15 april, 1990 until 3 december, 1996 who had a p ... | 2000 | 12497095 |
| somatotrophic and thyroid hormones around the onset of lay in broiler breeders under different conditions. | somatotrophic and thyroid hormones were determined around the onset of reproduction in broiler breeders reared in two different housing systems [dark, close-sided house (ch) and conventional, open-sided house (oh)]. in both groups age-related changes were obvious for thyroxine (t4), growth hormone (gh) and insulin-like growth factor (igf-1); levels of t4 decreased, especially between 24 and 28 weeks in both groups; concomitantly gh sharply increased over the same period. a transient peak in trii ... | 2002 | 12498147 |
| low recurrence rate of hepatocellular carcinoma after liver transplantation: better patient selection or lower immunosuppression? | liver transplantation is currently offered to a limited number of patients with hepatocellular carcinoma (hcc) because of strict criteria introduced in the past to avoid recurrence. immunosuppression represents a risk factor for tumor growth; the schedules of the immunosuppressant drugs have been modified through the years, aiming to reduce their dosage to the effective minimum. | 2002 | 12499891 |
| [molecular genetic progression on nasopharyngeal carcinoma]. | the aim of this paper was to discuss the molecular genetic events in the development and progression of nasopharyngeal carcinoma (npc), and the correlation between genetic alterations and clinicopathological changes of npc. analyzed by loh (loss of heterozygosity) and cgh (comparative genomic hybridization) in primary npc, high frequent allelic losses were observed on chromosomes 1p, 3p, 9p, 9q, 11q, 13q, 14q, 16q, and 19q, and the minimum deletion regions were also localized; there are closely ... | 2002 | 12500388 |
| bacteriophage t4 dam dna-(n6-adenine)-methyltransferase. processivity and orientation to the methylation target. | we carried out steady state and pre-steady state (burst) kinetic analyses of the bacteriophage t4 dam dna-(n(6)-adenine)-methyltransferase (mtase)-mediated methyl group transfer from s-adenosyl-l-methionine (adomet) to ade in oligonucleotide duplexes containing one or two specific gatc sites with different combinations of methylated and unmodified targets. we compared the results for ligated 40-mer duplexes with those of the mixtures of the two unligated duplexes used to generate the 40-mers. th ... | 2003 | 12501249 |
| purification of a chitinase from trichoderma sp. and its action on sclerotium rolfsii and rhizoctonia solani cell walls. | trichoderma harzianum is an effective biocontrol agent of several important plant pathogenic fungi. this trichoderma species attacks other fungi by secreting lytic enzymes, including beta-1,3-glucanase and chitinolytic enzymes. superior biocontrol potential may then be found in strains having a high capacity to produce these enzymes. we have therefore evaluated the capacity of six unidentified trichoderma spp. isolates to produce chitinolytic enzymes and beta-1,3-glucanases in comparison with t. ... | 1997 | 12501351 |
| thermal transition profiles of bacteriophage t4 and its dna. | | 1999 | 12501385 |
| coexistence of thyrotropin-producing pituitary adenoma with papillary adenocarcinoma of the thyroid--a case report and surgical strategy. | we report a very rare case of thyrotropin (thyroxin stimulating hormone, tsh)-producing pituitary adenoma coexisting with a papillary adenocarcinoma of the thyroid. a 45-year-old woman presented with hyperhidrosis and a nodule in the left thyroid that was first noticed one year earlier. an endocrinological examination showed elevated serum levels of free triiodothyronine (t3) and free throxin (t4) without inhibition of tsh, suggesting the presence of syndromes of inappropriate secretion of tsh. ... | 2001 | 12501979 |
| affinity precipitation separation of dna binding protein using block conjugate composed of poly(n-isopropylacrylamide) grafted double-stranded dna and double-stranded dna containing a target sequence. | in this research, we synthesized a novel dna-polymer conjugate and evaluated its application to an affinity precipitation separation of tata-box binding protein (tbp), which is a representative general transcription factor. the conjugate was composed of two fractions. one was a double-stranded dna modified by the grafting of poly(n-isopropylacrylamide) (pnipaam), which is known as a thermosensitive vinyl polymer. the other fraction is a native double-stranded dna containing a specific base seque ... | 2002 | 12502078 |
| purification of histidine-tagged t4 rna ligase from e. coli. | here we report the construction of a histidine-tagged t4 rna ligase expression plasmid (prht4). the construct, when overexpressed in bl21 (de3) cells, allows the preparation of large quantities of t4 rna ligase in high purity using only a single purification column. the histidine affinity tag does not inhibit enzyme function, and we were able to purify 1-3 mg pure protein/g cell pellet. a simple purification procedure ensures that the enzyme is de-adenylated to levels comparable to those found f ... | 2002 | 12503310 |
| thyroid dysfunction and high thyroid stimulating hormone levels in children with down's syndrome. | in order to delineate the spectrum of thyroid abnormalities in children with down's syndrome (ds), first visit height data (sds) and serum tsh, t4 and antiperoxidase antibodies concentrations were retrospectively evaluated in 137 children (71 girls) with ds (0.04-16 years). results: congenital hypothyroidism was detected in 2.9% of patients. thyroid disease occurred in 9%: four hyperthyroidism and eight hypothyroidism. overt thyroid disease was always related to thyroid autoimmunity. the remaini ... | 2002 | 12503863 |
| sites of local recurrence after surgery, with or without chemotherapy, for rectal cancer: implications for radiotherapy field design. | to examine the sites of pelvic recurrence in patients with rectal cancer previously untreated with radiotherapy to determine the relative frequency and location of recurrence within the pelvis. | 2003 | 12504046 |
| time course of gag protein assembly in hiv-1-infected cells: a study by immunoelectron microscopy. | recent biochemical studies have identified high molecular complexes of the hiv gag precursor in the cytosol of infected cells. using immunoelectron microscopy we studied the time course of the synthesis and assembly of a hiv gag precursor protein (pr55gag) in sf9 cells infected with recombinant baculovirus expressing the hiv gag gene. we also immunolabeled for pr55gag human t4 cells acutely or chronically infected with hiv-1. in sf9 cells, the time course study showed that the first gag protein ... | 2003 | 12504555 |
| shortened irradiation scheme, continuous infusion of 5-fluorouracil and fractionation of mitomycin c in locally advanced anal carcinomas. results of a phase ii study of the european organization for research and treatment of cancer. radiotherapy and gastrointestinal cooperative groups. | the european organization for research and treatment of cancer (eortc) 22861 randomised trial established that combined radiochemotherapy is the standard treatment for locally advanced anal cancer. this eortc phase ii study (#22953) tests the feasibility of reducing the gap between sequences to 2 weeks, to deliver mitomycin c (mmc) in each radiotherapy sequence and 5-fu continuously during the treatment. the first sequence consisted of 36 gy over 4 weeks. 5-fu 200 mg/m(2)/days 1-26, mmc 10 mg/m( ... | 2003 | 12504657 |
| depression and anxiety in hyperthyroidism. | our objective was to determine symptomatology of depression and anxiety in patients with untreated hyperthyroidism and compare with euthyroid patients. | 2002 | 12505101 |
| the cell-layer- and cell-type-specific distribution of galnac-transferases in the ocular surface epithelia is altered during keratinization. | it has been hypothesized that the biosynthesis of o-linked glycans on proteins, particularly on the highly o-glycosylated mucins, by the corneal and conjunctival epithelium is necessary for the protection and maintenance of a healthy ocular surface. the initial step in o-glycosylation is the enzymatic addition of n-acetyl galactosamine (galnac) to serine and threonine residues by a large family of polypeptide galnac-transferases (galnac-ts). the purpose of this study was to determine the cellula ... | 2003 | 12506059 |
| [action of spirulina platensis on bacterial viruses]. | the impact of the biomass of the blue-green microalga (cyanobacterium) s. platensis on bacteriophage t4 (bacterial virus) has been evaluated. the study revealed that the addition of s. platensis biomass into the agar nutrient medium, followed by sterilization with 2% chloroform and thermal treatment, produced an inhibiting or stimulating effect on the reproduction of the bacteriophage in escherichia coli b cells, depending on the concentration of s. platensis and the multiplicity of phage infect ... | 2002 | 12506621 |
| [a case of hypothyroidism due to hashimoto disease that presented elevations of free t3 and free t4 by thyroid hormone autoantibodies]. | when we commonly evaluate the thyroid function, we measure tsh, free t3 and free t4. however, there are some cases that are difficult to diagnose because of the existence of thyroid hormone autoantibodies. we experienced a case of a 14-year-old girl with diffuse struma whose tsh, free t3 and free t4 were elevated by detected with amerlex m free t3 and free t4 kits, although she did not have any symptoms. as the free t4 level was low by the equilibrium dialysis method, we diagnosed this case as h ... | 2002 | 12506859 |
| human donor bone marrow cells induce in vitro "suppressor t cells" that functionally suppress autologous b cells. | we have reported a beneficial effect of donor vertebral body bone marrow cells (dbmc) infusions in cadaver renal allograft recipients in a 6-year follow-up, but with a transient increase in early (6 month) postoperative cmv infections and concomitant suppressed immunoglobulins (ig) production. we also found that although there was no difference between the dbmc-infused and non-infused (control) groups in the development of donor-specific antibody, we now describe an additional difference seen in ... | 2003 | 12507811 |
| [lymph node metastases in paranasal sinus carcinoma: prognostic value and treatment]. | the purpose of this report is to assess, on the basis of a sizeable study, the prognostic value of lymph node metastases in paranasal sinus carcinoma and, in particular, in squamous cell carcinoma of the maxillary sinus. we have reviewed the charts of 601 cases of paranasal sinus carcinoma between 1970 and 1999. all of the patients were treated surgically, alone or associated with chemotherapy and/or radiotherapy. the maxillary sinus tumors numbered 379 (153 squamous cell carcinomas, 15 undiffer ... | 2002 | 12510338 |
| estimation of energy balance at the individual and herd level using blood and milk traits in high-yielding dairy cows. | this study aimed to estimate individual and herd-level energy balance (eb) using blood and milk traits in 90 multiparous high-yielding holstein cows, held on a research farm, from wk 1 to 10 postpartum (p.p.) and to investigate the precision of prediction with successively decreased data sets simulating smaller herd sizes and with pooled samples. dry matter intake, milk yield, and bw were measured daily from parturition through wk 10 p.p. milk composition was determined 4 times per week, and mil ... | 2002 | 12512605 |
| endoscopic transthoracic sympathectomy for upper limb hyperhidrosis: limited sympathectomy does not reduce postoperative compensatory sweating. | compensatory sweating is the most common and troublesome complication of thoracodorsal sympathectomy. whether the magnitude of compensatory sweating is related to the extent of sympathectomy is unclear. we investigated the association between the extent of sympathectomy and the occurrence and severity of compensatory sweating after endoscopic transthoracic sympathectomy for upper limb hyperhidrosis. | 2003 | 12514588 |
| [effect of haidonghua powder(hdhp) on hypothalamic obesity in rats]. | to study the effect of hdhp including laminaria japonica aresch. and benincasa hispida(thunb.) cogn. etc. on antiobesity in rats with hypothalamic obesity. | 2000 | 12515214 |
| [preoperative diclofenac does not reduce pain of laparoscopic cholecystectomy]. | to study the safety and efficacy of diclofenac for preemptive analgesia in the first 24 hours after elective laparoscopic cholecystectomy (elc). | 2002 | 12516489 |
| response to preoperative chemoradiation increases the use of sphincter-preserving surgery in patients with locally advanced low rectal carcinoma. | although controversial, some believe that preoperative chemoradiation increases the use of sphincter-preserving surgery in low rectal carcinoma patients. this article investigates the relationship between objective tumor response and sphincter preservation in low rectal carcinoma patients. | 2003 | 12518377 |
| a population-based study of thyroid function after radiotherapy and chemotherapy for a childhood brain tumor. | the effect of craniospinal irradiation (csi) vs. cranial irradiation (cir) only with or without chemotherapy (ct) on the hypothalamus/pituitary (hp) thyroid axis was assessed in a population-based study of patients treated for a childhood brain tumor not directly involving the hp axis. thyroid function was evaluated and compared with that in healthy controls (n = 27), measuring tsh, free t4, total t4, total t3, and trh. the biological effective dose (bed) of radiotherapy, determined for the hp r ... | 2003 | 12519842 |
| cardiovascular safety of acute recombinant human thyrotropin administration to patients monitored for differentiated thyroid cancer. | eleven patients who had undergone total thyroidectomy for differentiated thyroid cancer and who were on chronic tsh-suppressive therapy with levothyroxine (l-t4), underwent 24-h holter electrocardiogram and doppler-echocardiography before and after acute recombinant human tsh (rhtsh) administration for disease staging. the treatment, which was generally well tolerated, did not affect circulating thyroid hormones levels, nor did it have measurable effects on heart rate, rhythm, left ventricular m ... | 2003 | 12519854 |
| luteal phase deficiency in recreational runners: evidence for a hypometabolic state. | exercising women with amenorrhea exhibit a hypometabolic state. the purpose of this study was to evaluate the relationship of luteal phase deficient (lpd) menstrual cycles to metabolic hormones, including thyroid, insulin, human gh (hgh), leptin, and igf-i and its binding protein levels in recreational runners. menstrual cycle status was determined for three consecutive cycles in sedentary and moderately active women. menstrual status was defined as ovulatory or lpd. subjects were either sedenta ... | 2003 | 12519874 |
| acute phase effects of atp-mgcl2 on experimental spinal cord injury. | the purpose of this study was to study the acute phase effects of adenosine triphosphate (atp)-mgcl2 on experimental spinal cord clip compression injury. spinal cord clip compression injury was performed on 36 albino wistar rats. the rats were divided into five groups. t4-t8 total laminectomy was performed on all rats. group 1: sham-operated group. group 2: clip compression group. in group 3, atp-mgcl2 (100 micro mol/kg) was given 2 min before the "clip compression injury." in group 4, atp-mgcl2 ... | 2003 | 12520320 |
| complementation of bacteriophage induction and recombination defects in escherichia coli reca(-) mutants by expression of the cloned t4 bacteriophage uvsx gene. | previous workers reported that the t4 bacteriophage uvsx protein could promote neither reca-lexa-mediated dna repair nor induction of lysogenized bacteriophage, only recombination. reexamination of these phenotypes demonstrated that, in contrast to these prior studies, when this gene was cloned into a medium but not a low-copy-number vector, it stimulated both a high frequency of spontaneous induction and mitomycin c-stimulated bacteriophage induction in a strain containing a reca13 mutation, bu ... | 2003 | 12520361 |
| t3-t4 disc herniation in a german shepherd dog. | | 2002 | 12521251 |
| quality of life 2-10 years after combined treatment for advanced oral and oropharyngeal cancer. | this study reports the findings of an evaluation of quality of life (qol) in long-term survivors of advanced oral and oropharyngeal cancer treated with preoperative chemoradiotherapy followed by surgery (combined treatment=multimodal therapy). all patients had t2-t4 tumours and all received locoregional radical resection and simultaneous microvascular reconstruction. from 1990 to 1998, 181 patients have been treated at the university hospital of cranio-maxillofacial and oral surgery in vienna. 1 ... | 2002 | 12521326 |
| [glia-like brain tumors. potential role of thyroid hormone and its nuclear receptors (tr)]. | over-proliferation of certain cells lead to tumor formation and therefore impairments of regulation of the cell cycle are inalienable and basic signs of neoplasms. the majority of known oncogenes and tumor suppressors are components of a few common signaling pathways that control the cell cycle, genome integrity, cell differentiation and apoptosis. changes in these signaling pathways cause the development of tumor genesis. the thyroid hormones (3, 5, 3'-l-triiodothyronine, t3; 3, 5, 3', 5'-l tet ... | 2002 | 12523122 |
| thyrotropin-secreting pituitary adenoma with growth hormone hypersecretion. | a 34-year-old woman suffered from palpitation, easy sweating, heat intolerance, increased appetite, irregular menstrual cycle and hand tremor for 1 year. thyroid function tests showed elevated serum thyroxine (t4), tri-iodothyronine (t3) and thyrotropin (tsh). computerized tomography (ct) revealed pituitary tumor with supraseller extension. thyrotropin releasing hormone (trh) test showed blunted tsh response with elevated baseline level and paradoxical growth hormone (gh) response with elevated ... | 2002 | 12523814 |
| interaction of double-stranded t4 dna with cationic gel of poly(diallyldimethylammonium chloride). | interaction between duplex t4 dna and a slightly cross-linked cationic gel of poly(diallyldimethylammonium chloride) in aqueous media was studied by fluorescent microscopy. while short dna chains such as plasmid dnas penetrate into the gel and form a phase of polyelectrolyte complex with the cationic network, the genomic giant dna chains of t4 phages form complexes only on local areas of the gel surface. the dna/gel complex exhibited different characteristic morphologies depending on the conditi ... | 2003 | 12523843 |
| transoral laser microsurgery for squamous cell carcinoma of the base of the tongue. | to determine the role of transoral laser microsurgery for base of tongue squamous cell carcinoma. | 2003 | 12525192 |
| thyroxine and tri-iodothyronine differently affect uncoupling protein-1 content and antioxidant enzyme activities in rat interscapular brown adipose tissue. | the activity of the antioxidant enzymes copper-zinc superoxide dismutase (cuznsod), manganese superoxide dismutase (mnsod) and catalase (cat), as well as mitochondrial glycerol-3-phosphate dehydrogenase (mgpdh) activity, uncoupling protein-1 (ucp1) content, catecholamine degrading enzyme monoamine oxidase (mao) activity and malonyl dialdehyde (mda) concentration were studied in rat interscapular brown adipose tissue (ibat). rats were treated with either thyroxine (t4) or tri-iodothyronine (t3) f ... | 2003 | 12525247 |
| primary chemotherapy in resectable oral cavity squamous cell cancer: a randomized controlled trial. | prognosis of patients with advanced oral cavity cancer is worth improving. chemotherapy has been reported to be especially active in oral cavity tumors. here we repeat the results of a randomized, multicenter trial enrolling patients with a resectable, stage t2-t4 (> 3 cm), n0-n2, m0 untreated, squamous cell carcinoma of the oral cavity. | 2003 | 12525526 |
| [expression of cyclooxygenase-2 in human transitional cell bladder carcinomas]. | cyclooxygenase(cox) is a rate-limiting enzyme in prostaglandin synthesis. recent study suggested that cox-2 was associated with carcinogenesis. this study was designed to investigate the role of cox in the development and progression of bladder cancer through examining the expression of cox-1 and cox-2 in human bladder cancer tissue, normal bladder mucosa, and cystitis tissue. | 2002 | 12526218 |
| [cloning and sequencing of fusion protein gene of recombinant gm-hbsag]. | gm-csf is an effective adjuvant for immunology, it was used to improve the immunological function of antigen and to increase the anti-hbs responses. through using polymerase chain reaction (pcr) technique, we amplified 384 bp and 846 bp gene fragments from gm-csf and hbsag. the products were extracted and purified; a 1230 bp gene fragment was obtained by t4 dna linkage. this amplified product was cloned into puc19 vector. the positive clones were screened and the inserted fragment was determined ... | 1998 | 12526340 |
| analysis of regions within the bacteriophage t4 asia protein involved in its binding to the sigma70 subunit of e. coli rna polymerase and its role as a transcriptional inhibitor and co-activator. | bacteriophage t4 asia, a protein of 90 amino acid residues, binds to the sigma(70) subunit of escherichia coli rna polymerase and inhibits host or t4 early transcription or, together with the t4 mota protein, activates t4 middle transcription. to investigate which regions within asia are involved in forming a complex with sigma(70) and in providing transcriptional functions we generated random mutations throughout asia and targeted mutations within the c-terminal region. we tested mutant protein ... | 2003 | 12527294 |
| guidelines on treatment of stage iiib non-small cell lung cancer. | stage iiib includes patients with t4, any n, m0, and any t, n3, m0. surgery may be indicated only for carefully selected t4n0m0 patients with or without neoadjuvant chemotherapy or chemoradiotherapy. patients with n3 lymph node involvement are not considered as surgical candidates. for patients with unresectable disease, good performance score, and minimal weight loss, treatment with combined chemotherapy and radiotherapy has resulted in better survival than treatment with radiotherapy alone. mu ... | 2003 | 12527581 |
| lung cancer. special treatment issues. | this chapter of the lung cancer guidelines addresses patients with particular forms of non-small cell lung cancer that require special considerations. this includes patients with pancoast tumors, t4n0,1m0 tumors, satellite nodules in the same lobe, synchronous and metachronous multiple primary lung cancers (mplc), and solitary metastases. for patients with a pancoast tumor, a multimodality approach, involving chemoradiotherapy and surgical resection, appears optimal provided appropriate staging ... | 2003 | 12527583 |
| resting thyroid and leptin hormone changes in women following intense, prolonged exercise training. | this study examined whether free (f) triidothyronine (t3), f thyroxine (t4), thyroid stimulating hormone (tsh), and leptin concentrations at rest changed in response to 20 weeks of exercise-training. two groups of women were recruited for participation in the study, collegiate athletes ( n=17) and sedentary controls (n=4). exercise training consisted of daily athletic activity such as rowing, running, and weight lifting. subjects were initially grouped into rowers and controls. however, earlier ... | 2003 | 12527982 |
| [gigantism with low serum level of growth hormone: a case report]. | gigantism with low or normal basal concentrations of growth hormone (gh) is a rare condition, possibly due to abnormal gh secretory patterns, enhanced tissue sensitivity to gh, or the existence of an unidentified growth promoting factor. here we report an 11 year-old female case of gigantism with a normal pituitary gland. her height was 181 cm, body weight 77 kg, and bone age 11.1 years. her basal serum gh levels were lower than 1 ng/ml. the levels of t3, t4, ft3, ft4, tsh, e2, lh, fsh, prl, ptc ... | 2001 | 12528568 |
| mechanism of chaperone function in small heat-shock proteins. phosphorylation-induced activation of two-mode binding in alphab-crystallin. | the consequences of alphab-crystallin phosphorylation on its chaperone activity were investigated using a detailed analysis of the recognition and binding of destabilized t4 lysozyme (t4l) mutants by alphab-crystallin phosphorylation mimics containing combinations of serine to aspartate substitutions. the t4l site-directed mutants were selected to constitute an energetic ladder of progressively destabilized proteins having similar structures in the folded state. alphab-crystallin and its variant ... | 2003 | 12529319 |
| the significance of tenascin-c serum level as tumor marker in squamous cell carcinoma of the head and neck. | tenascin, an extracellular matrix glycoprotein, is transiently present in embryonic tissue, in benign granulation tissue, but also in several highly anaplastic tumors like fibrosarcoma, melanoma and squamous cell carcinoma of the skin. this study was performed to validate elevated tenascin serum levels as a possible marker for head and neck squamous cell carcinomas (hnscc). | 2002 | 12530049 |
| associations between infections and fatigue in a dutch working population: results of the maastricht cohort study on fatigue at work. | the relationship between fatigue and common infections was further explored, as part of a 3 year prospective cohort study on fatigue at work. the current study is based on seven successive questionnaires, covering the first 2 years of follow-up. the overall response at baseline was 45% (n = 12,140). on t1 10,592 (87.2% compared to baseline response) employees returned the questionnaire. for t2, t3, t4, t5 and t6, 10,270 (84.6%), 9655 (79.5%), 8956 (73.8%), 8692 (71.6%) and 8070 (66.5%) employees ... | 2001 | 12530766 |
| mechanisms underlying the cardiovascular responses to spinal dopamine receptor stimulation by apomorphine in anesthetized rats. | the present study investigated the mechanisms by which intrathecal (i.t.) apomorphine affects mean aortic pressure and heart rate in anesthetized rats. in saline-pretreated rats, upper thoracic (t2-t4) i.t. administration of apomorphine (48 microg/rat) induced immediate and significant hypotension and bradycardia. these responses were unaffected by intravenous (i.v.) methylatropine (1 mg/kg) or bilateral vagotomy, while they were prevented by i.t. lidocaine (25 microl at 1%) or i.v. hexamethoniu ... | 2003 | 12531464 |
| endoscopic ultrasonography in preoperative staging of gastric cancer: determination of tumor invasion depth, nodal involvement and surgical resectability. | current study was aimed to evaluate the usefulness of eus in tnm staging of gastric cancer by comparing eus preoperative staging with pathological findings, and the preliminary exploration of possible reasons for overstaging and understaging phenomenon was especially intended. | 2003 | 12532442 |
| sizing the holin lesion with an endolysin-beta-galactosidase fusion. | double-stranded dna phages require two proteins for efficient host lysis: the endolysin, a muralytic enzyme, and the holin, a small membrane protein. in an event that defines the end of the vegetative cycle, the lambda holin s acts suddenly to permeabilize the membrane. this permeabilization enables the r endolysin to attack the cell wall, after which cell lysis occurs within seconds. a c-terminal fusion of the r endolysin with full-length beta-galactosidase (beta-gal) was tested for lytic compe ... | 2003 | 12533453 |
| escherichia coli endoribonucleases involved in cleavage of bacteriophage t4 mrnas. | the dmd mutant of bacteriophage t4 has a defect in growth because of rapid degradation of late-gene mrnas, presumably caused by mutant-specific cleavages of rna. some such cleavages can occur in an allele-specific manner, depending on the translatability of rna or the presence of a termination codon. other cleavages are independent of translation. in the present study, by introducing plasmids carrying various soc alleles, we could detect cleavages of soc rna in uninfected cells identical to thos ... | 2003 | 12533474 |