| enhanced antifungal and insect α-amylase inhibitory activities of alpha-tvd1, a peptide variant of tephrosia villosa defensin (tvd1) generated through in vitro mutagenesis. | tvd1 is a small, cationic, and highly stable defensin from the weedy legume, tephrosia villosa with demonstrated in vitro antifungal activity. we show here peptide modifications in tvd1 that lead to enhanced antifungal activities. three peptide variants, s32r, d37r, and alpha-tvd1 (-g-m-t-r-t-) with variations in and around the β2-β3 loop region that imposes the two β-strands, β2 and β3 were generated through in vitro mutagenesis. alpha-tvd1 exhibited enhanced antifungal activity against the fun ... | 2012 | 22244814 |
| identification of different fusarium spp. in allium spp. in germany. | in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ... | 2015 | 27141742 |
| occurrence of root rot and vascular wilt diseases in roselle (hibiscus sabdariffa l.) in upper egypt. | roselle (hibiscus sabdariffa l.) family malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. roselle is cultivated mainly in upper egypt (qena and aswan governorates) producing 94% of total production. root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. the main objective of the present study is to identify and characterize pathogens associated with r ... | 2014 | 24808737 |
| molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran. | fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ... | 2011 | 23961146 |
| veterinary fusarioses within the united states. | multilocus dna sequence data were used to assess the genetic diversity and evolutionary relationships of 67 fusarium strains from veterinary sources, most of which were from the united states. molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. the majority of the veterinary isolates (47/67 = 70.1%) were nested within the fusarium solani sp ... | 2016 | 27605713 |
| spectrum of fusarium infections in tropical dermatology evidenced by multilocus sequencing typing diagnostics. | fusarium species are emerging causative agents of superficial, cutaneous and systemic human infections. in a study of the prevalence and genetic diversity of 464 fungal isolates from a dermatological ward in thailand, 44 strains (9.5%) proved to belong to the genus fusarium. species identification was based on sequencing a portion of translation elongation factor 1-alpha (tef1-α), rdna internal transcribed spacer and rna-dependent polymerase subunit ii (rpb2). our results revealed that 37 isolat ... | 2015 | 25530264 |
| molecular analyses of fusarium isolates recovered from a cluster of invasive mold infections in a brazilian hospital. | invasive fusariosis (if) is a rare but often fatal fungal infection in immunosuppressed patients. in 2007, cases of if above the expected epidemiologic baseline were detected in the hematology ward of a hospital in rio de janeiro, brazil. possible sources of infection were investigated by performing environmental sampling and patient isolate collection, followed by molecular typing. isolates from dermatology patients with superficial fusariosis were included in the study for comparison to molecu ... | 2013 | 23363475 |
| rapid identification of fusarium graminearum species complex using rolling circle amplification (rca). | rolling circle amplification (rca) of dna is a sensitive and cost effective method for the rapid identification of pathogenic fungi without the need for sequencing. amplification products can be visualized on 1% agarose gel to verify the specificity of probe-template binding or directly by adding fluorescent dyes. fusarium head blight (fhb) is currently the world's largest threat to the production of cereal crops with the production of a range of mycotoxins as an additional risk. we designed set ... | 2012 | 22326479 |
| comparison of dna microarray, loop-mediated isothermal amplification (lamp) and real-time pcr with dna sequencing for identification of fusarium spp. obtained from patients with hematologic malignancies. | the performance of three molecular biology techniques, i.e., dna microarray, loop-mediated isothermal amplification (lamp), and real-time pcr were compared with dna sequencing for properly identification of 20 isolates of fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. dna microarray, lamp and real-time pcr identified 16 (80%) out of 20 samples as fusarium solani species complex (fssc) and four (20%) as fusarium spp. t ... | 2017 | 28324245 |
| involvement of salicylic acid, ethylene and jasmonate acid signaling pathways in susceptibility of tomato for fusarium oxysporum. | phytohormones such as salicylic acid (sa), ethylene (et) and jasmonic acid (ja) play key roles in plant defense following pathogen attack. involvement of these hormones in susceptibility following fusarium oxysporum (fo) infection has mostly been studied in arabidopsis thaliana. fo, however, causes vascular wilt disease in a broad range of crops, including tomato (solanum lycopersicum). surprisingly little is known about the involvement of these phytohormones in susceptibility of tomato towards ... | 2017 | 28390170 |
| rapd assisted selection of black gram (vigna mungo l. hepper) towards the development of multiple disease resistant germplasm. | black gram (vigna mungo l. hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. the yield potential of black gram is shallow due to lack of genetic variability and biotic stress susceptibility. core biotic stress factors include mung bean yellow mosaic virus (mymv), urdbean leaf crinkle virus (uclv), wilt (fusarium oxysporum) and powdery mildew (erysiphe polygoni dc). although many studies determine resistant varieties to a parti ... | 2017 | 28389895 |
| investigation of genetic diversity of fusarium oxysporum f. sp. fragariae using pcr-rflp. | fusarium wilts of strawberry, caused by fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. reliable chemical control of strawberry fusarium wilt disease is not yet available. moreover, other well-known f. oxysporum have different genetic information from f. oxysporum f. sp. fragariae. this analysis investigates the genetic diversity of strawberry fusairu ... | 2017 | 28381961 |
| fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems. | the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ... | 2017 | 28369915 |
| transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants. | fusarium oxysporum f. sp. melonis snyd. & hans race 1.2 (fom1.2) is the most virulent and yield-limiting pathogen of melon (cucumis melo l.) worldwide. current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. rna-seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-fom1.2 interaction in the resistant doubled haploid line nad and in the ... | 2017 | 28367157 |
| antifungal activity, yield, and composition of ocimum gratissimum essential oil. | ocimum gratissimum l. or clove basil, belongs to the lamiaceae family, has various desirable uses and applications. beyond its aromatic, seasoning, and medicinal applications, this plant also has antimicrobial activity. this study was aimed at assessing the antifungal activity, yield, and composition of the essential oil (eo) of o. gratissimum. the species was cultivated in garden beds with dystrophic red latosol soil type containing high organic-matter content. the eo was obtained by hydrodisti ... | 2017 | 28362991 |
| members of gammaproteobacteria as indicator species of healthy banana plants on fusarium wilt-infested fields in central america. | culminating in the 1950's, bananas, the world's most extensive perennial monoculture, suffered one of the most devastating disease epidemics in history. in latin america and the caribbean, fusarium wilt (fw) caused by the soil-borne fungus fusarium oxysporum f. sp. cubense (foc), forced the abandonment of the gros michel-based export banana industry. comparative microbiome analyses performed between healthy and diseased gros michel plants on fw-infested farms in nicaragua and costa rica revealed ... | 2017 | 28345666 |
| concurrent methylation and demethylation of arsenic by fungi and their differential expression in the protoplasm proteome. | microbial methylation and demethylation are central to arsenic's (as) biogeochemical cycling. here, the transformations of monomethylarsonic acid (mma(v)) (50 mg l(-1)) for 15 days in cells of as-methylating fungi, fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1, were evaluated, and trace concentrations of as(iii) and as(v) were observed in fungal cell extracts. trace amounts of dma(v) were also detected in mma(v) and p. janthinellum sm-12f4 incuba ... | 2017 | 28336093 |
| effector gene suites in some soil isolates of fusarium oxysporum are not sufficient predictors of vascular wilt in tomato. | seventy-four f. oxysporum soil isolates were assayed for known effector genes present in an f. oxysporum f.sp. lycopersici race 3 tomato wilt strain (fol mn-25) obtained from the same fields in manatee county, florida. based on the presence or absence of these genes, four haplotypes were defined, two of which represented 96% of the surveyed isolates. these two most common effector haplotypes contained either all or none of the assayed race 3 effector genes. we hypothesized that soil isolates wit ... | 2017 | 28323535 |
| draft genome sequence of rhizobium sp. strain tbd182, an antagonist of the plant-pathogenic fungus fusarium oxysporum, isolated from a novel hydroponics system using organic fertilizer. | rhizobium sp. strain tbd182, isolated from a novel hydroponics system, is an antagonistic bacterium that inhibits the mycelial growth of fusarium oxysporum but does not eliminate the pathogen. we report the draft genome sequence of tbd182, which may contribute to elucidation of the molecular mechanisms of its fungistatic activity. | 2017 | 28302768 |
| nitrate increased cucumber tolerance to fusarium wilt by regulating fungal toxin production and distribution. | cucumber fusarium wilt, induced by fusarium oxysporum f. sp. cucumerinum (foc), causes severe losses in cucumber yield and quality. nitrogen (n), as the most important mineral nutrient for plants, plays a critical role in plant-pathogen interactions. hydroponic assays were conducted to investigate the effects of different n forms (nh₄⁺ vs. no₃(‒)) and supply levels (low, 1 mm; high, 5 mm) on cucumber fusarium wilt. the no₃(‒)-fed cucumber plants were more tolerant to fusarium wilt compared with ... | 2017 | 28287458 |
| medium ph in submerged cultivation modulates differences in the intracellular protein profile of fusarium oxysporum. | fusarium oxysporum is a filamentous fungus that damages a wide range of plants and thus causes severe crop losses. in fungal pathogens, the genes and proteins involved in virulence are known to be controlled by environmental ph. here, we report the influence of culture-medium ph (5, 6, 7, and 8) on the production of degradative enzymes involved in the pathogenesis of f. oxysporum urm 7401 and on the 2d-electrophoresis profile of intracellular proteins in this fungus. f. oxysporum urm 7401 was gr ... | 2017 | 28281888 |
| the plasma membrane-localized sucrose transporter ibsweet10 contributes to the resistance of sweet potato to fusarium oxysporum. | sweet (sugars will eventually be exported transporter) proteins, a novel family of sugar transporters, mediate the diffusion of sugars across cell membranes and acts as key players in sucrose phloem loading. manipulation of sweet genes in plants leads to various effects on resistance to biotic and abiotic stresses due to disruption of sugar efflux and changes in sugar distribution. in this study, a member of the sweet gene family, ibsweet10, was cloned from the sweet potato line nd98. mrna expre ... | 2017 | 28261250 |
| response of atnpr1-expressing cotton plants to fusarium oxysporum f. sp. vasinfectum isolates. | in our earlier investigation, we had demonstrated that transgenic cotton plants expressing atnpr1 showed significant tolerance to fusarium oxysporum f. sp. vasinfectum, isolate 11 (fov11) and several other pathogens. the current study was designed to further characterize the nature of the protection provided by atnpr1 expression and its limitations. green fluorescent protein-expressing fov11 was generated and used to study the progression of the disease within the plant. the results show that th ... | 2017 | 28250590 |
| identification of biomarkers for resistance to fusarium oxysporum f. sp. cubense infection and in silico studies in musa paradisiaca cultivar puttabale through proteomic approach. | panama wilt caused by fusarium oxysporum f. sp. cubense (foc) is one of the major disease constraints of banana production. previously, we reported the disease resistance musa paradisiaca cv. puttabale clones developed from ethylmethanesulfonate and foc culture filtrate against foc inoculation. here, the same resistant clones and susceptible clones were used for the study of protein accumulation against foc inoculation by two-dimensional gel electrophoresis (2-de), their expression pattern and a ... | 2016 | 28248219 |
| antimicrobial activity of medicinal plants correlates with the proportion of antagonistic endophytes. | medicinal plants are known to harbor potential endophytic microbes, due to their bioactive compounds. in a first study of ongoing research, endophytic bacteria were isolated from two medicinal plants, hypericum perforatum and ziziphora capitata with contrasting antimicrobial activities from the chatkal biosphere reserve of uzbekistan, and their plant-specific traits involved in biocontrol and plant growth promotion were evaluated. plant extracts of h. perforatum exhibited a remarkable activity a ... | 2017 | 28232827 |
| suppression of fusarium wilt of cucumber by ammonia gas fumigation via reduction of fusarium population in the field. | cucumber plants subjected to consecutive monoculture for 9 years were found to suffer from severe fusarium wilt disease, caused by the soil-borne fungus fusarium oxysporum f. sp. cucumerinum j. h. owen. in the present study, greenhouse experiments were performed to evaluate the influence of ammonia gas fumigation on fusarium wilt suppression, fungal abundance and fungal community composition. results showed that ammonia gas fumigation remarkably reduced disease incidence from 80% to 27%, resulti ... | 2017 | 28230182 |
| morphological and molecular characterization of fusarium spp pathogenic to pecan tree in brazil. | the occurrence of fusarium spp associated with pecan tree (carya illinoinensis) diseases in brazil has been observed in recent laboratory analyses in rio grande do sul state. thus, in this study, we i) obtained fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these fusarium isolates to pecan; iii) characterized and grouped fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified fusarium spp to the species ... | 2014 | 25501150 |
| wildly growing asparagus (asparagus officinalis l.) hosts pathogenic fusarium species and accumulates their mycotoxins. | asparagus officinalis l. is an important crop in many european countries, likely infected by a number of fusarium species. most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. however, there is lack of information on fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. therefore, the main scientific aim of this study was to identify the fusarium species and quantify their typical mycotoxins ... | 2016 | 26687343 |
| [overexpression, homology modeling and coenzyme docking studies of the cytochrome p450nor2 from cylindrocarpon tonkinense]. | cytochrome p450nor catalyzes an unusual reaction that transfers electrons from nadp/nadph to bound heme directly. to improve the expression level of p450nor2 from cylindrocarpon tonkinense (c.p450nor2), escherichia coli system was utilized to substitute the yeast system we constructed for expression of the p450nor2 gene, and the protein was purified in soluble form using ni(+)-nta affinity chromatography. in contrast to p450nor from fusarium oxysporum (f.p450nor) and p450nor1 from cylindrocarpon ... | 2016 | 27239859 |
| multiple garlic (allium sativum l.) micrornas regulate the immunity against the basal rot fungus fusarium oxysporum f. sp. cepae. | the basal plate rot fungus, fusarium oxysporum f. sp. cepae (foc), is the most devastating pathogen posing a serious threat to garlic (allium sativum l.) production worldwide. micrornas (mirnas) are key modulators of gene expression related to development and defense responses in eukaryotes. however, the mirna species associated with garlic immunity against foc are yet to be explored. in the present study, a small rna library developed from foc infected resistant garlic line was sequenced to ide ... | 2017 | 28224922 |
| effectiveness of neutral electrolyzed water on incidence of fungal rot on tomato fruits ( solanum lycopersicum l.). | we assessed the effect of neutral electrolyzed water (new) on the incidence of rot on tomato ( solanum lycopersicum l.) fruits inoculated with fusarium oxysporum , galactomyces geotrichum , and alternaria sp. at sites with lesions. the inoculated fruits were treated with new at 10, 30, and 60 mg liter(-1) active chlorine, with copper oxychloride fungicide, and with sterile distilled water (control) for 3, 5, and 10 min. in the experiment with f. oxysporum , 50 to 80% of the control fruits and 50 ... | 2016 | 28221850 |
| expression and distribution of extensins and agps in susceptible and resistant banana cultivars in response to wounding and fusarium oxysporum. | banana fusarium wilt caused by fusarium oxysporum f. sp. cubense (foc) is soil-borne disease of banana (musa spp.) causing significant economic losses. extensins and arabinogalactan proteins (agps) are cell wall components important for pathogen defence. their significance for foc resistance in banana was not reported so far. in this study, two banana cultivars differing in foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and agps aft ... | 2017 | 28218299 |
| rapid and sensitive diagnoses of dry root rot pathogen of chickpea (rhizoctonia bataticola (taub.) butler) using loop-mediated isothermal amplification assay. | dry root rot (drr) caused by the fungus rhizoctonia bataticola (taub.) butler, is an emerging disease in chickpea. the disease is often mistaken with other root rots like fusarium wilt, collar rot and black root rot in chickpea. therefore, its timely and specific detection is important. current detection protocols are either based on mycological methods or on protocols involving dna amplification by polymerase chain reaction (pcr). here we report the rapid and specific detection of r. bataticola ... | 2017 | 28218268 |
| draft genome sequence of an isolate of fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt of eggplant. | here, we present the genome sequence of an isolate (14004) of fusarium oxysporum f. sp. melongenae, an eggplant pathogen. the final assembly consists of 1,631 scaffolds with 53,986,354 bp (g+c content, 46.4%) and 16,485 predicted genes. | 2017 | 28209821 |
| paenibacillus polymyxa nsy50 suppresses fusarium wilt in cucumbers by regulating the rhizospheric microbial community. | paenibacillus polymyxa (p. polymyxa) nsy50, isolated from vinegar residue substrate, suppresses the growth of fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. the aim of the present study was to evaluate the effects of nsy50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to fusarium oxysporum. bacterial and fungal communities were investigated by illumina sequencing of t ... | 2017 | 28198807 |
| potential of burkholderia seminalis tc3.4.2r3 as biocontrol agent against fusarium oxysporum evaluated by mass spectrometry imaging. | species of genus burkholderia display different interaction profiles in the environment, causing either several diseases in plants and animals or being beneficial to some plants, promoting their growth, and suppressing phytopathogens. burkholderia spp. also produce many types of biomolecules with antimicrobial activity, which may be commercially used to protect crops of economic interest, mainly against fungal diseases. herein we have applied matrix-assisted laser desorption/ionization mass spec ... | 2017 | 28194740 |
| development of a hydrolysis probe-based real-time assay for the detection of tropical strains of fusarium oxysporum f. sp. cubense race 4. | fusarium oxysporum f. sp. cubense (foc) is one of the most important threats to global banana production. strategies to control the pathogen are lacking, with plant resistance offering the only long-term solution, if sources of resistance are available. prevention of introduction of foc into disease-free areas thus remains a key strategy to continue sustainable banana production. in recent years, strains of foc affecting cavendish bananas have destroyed plantations in a number of countries in as ... | 2017 | 28178348 |
| a rare case of subcutaneous fusariosis in an immunocompetent patient. | fusarium species can cause diseases in immunocompromised patients, whereas have rarely been reported as pathogens in immunocompetent individuals. fusarium oxysporum and f. solani species complex are the most frequent pathogenic species. primary subcutaneous hyalohyphomycosis due to these filamentous fungi is an extremely rare pathology, especially in immunocompetent patients. only 37 cases of deep fusarium infection in healthy patient have been reported in international literature. we report a c ... | 2017 | 28166614 |
| small rnas regulate the biocontrol property of fluorescent pseudomonas strain psd. | the production of biocontrol factors by pseudomonads is reported to be controlled at the post-transcriptional level by the gacs/gaca signal transduction pathway. this involves rna-binding translational repressor proteins, rsma and rsme, and the small regulatory rnas (srnas) rsmx, rsmy, and rsmz. while the former represses genes involved in secondary metabolite production, the latter relieves this repression at the end of exponential growth. we have studied the fluorescent pseudomonas strain psd, ... | 2017 | 28164793 |
| methyl salicylate level increase in flax after fusarium oxysporum infection is associated with phenylpropanoid pathway activation. | flax (linum usitatissimum) is a crop plant valued for its oil and fiber. unfortunately, large losses in cultivation of this plant are caused by fungal infections, with fusarium oxysporum being one of its most dangerous pathogens. among the plant's defense strategies, changes in the expression of genes of the shikimate/phenylpropanoid/benzoate pathway and thus in phenolic contents occur. among the benzoates, salicylic acid, and its methylated form methyl salicylate play an important role in regul ... | 2016 | 28163709 |
| development of mesorhizobium ciceri-based biofilms and analyses of their antifungal and plant growth promoting activity in chickpea challenged by fusarium wilt. | biofilmed biofertilizers have emerged as a new improved inoculant technology to provide efficient nutrient and pest management and sustain soil fertility. in this investigation, development of a trichoderma viride-mesorhizobium ciceri biofilmed inoculant was undertaken, which we hypothesized, would possess more effective biological nitrogen fixing ability and plant growth promoting properties. as a novel attempt, we selected mesorhizobium ciceri spp. with good antifungal attributes with the assu ... | 2017 | 28148979 |
| trichoderma harzianum containing 1-aminocyclopropane-1-carboxylate deaminase and chitinase improved growth and diminished adverse effect caused by fusarium oxysporum in soybean. | an isolate, named trichoderma harzianum t-soybean, showed growth-promoting for soybean seedlings and induced resistance to fusarium oxysporum under greenhouse. compared to control soybean seedlings, fresh weight, dry weight, lateral root number, chlorophyll content, root activity and soluble protein of plants pretreated with t-soybean increased, but initial pod height reduced. furthermore, we found that t-soybean inhibited the growth of f. oxysporum by parasitic function. in addition, plate test ... | 2017 | 28135657 |
| synthesis and antifungal activity of novel myrtenal-based 4-methyl-1,2,4-triazole-thioethers. | a series of novel myrtenal derivatives bearing 1,2,4-triazole moiety were designed and synthesized by multi-step reactions in an attempt to develop potent antifungal agents. their structures were confirmed by using uv-vis, ftir, nmr, and esi-ms analysis. antifungal activity of the target compounds was preliminarily evaluated by the in vitro method against fusarium oxysporum f. sp. cucumerinum, physalospora piricola, alternaria solani, cercospora arachidicola, and gibberella zeae at 50 µg/ml. com ... | 2017 | 28125042 |
| discovery of fungal denitrification inhibitors by targeting copper nitrite reductase from fusarium oxysporum. | the efficient application of nitrogenous fertilizers is urgently required, as their excessive and inefficient use is causing substantial economic loss and environmental pollution. a significant amount of applied nitrogen in agricultural soils is lost as nitrous oxide (n2o) in the environment due to the microbial denitrification process. the widely distributed fungus fusarium oxysporum is a major denitrifier in agricultural soils and its denitrification activity could be targeted to reduce nitrog ... | 2017 | 28117584 |
| novel 1,2,3-triazolium-functionalized starch derivatives: synthesis, characterization, and evaluation of antifungal property. | four novel 1,2,3-triazolium-functionalized starch derivatives were synthesized by n-alkylating the precursor starch derivatives with 1,2,3-triazole with iodomethane based on cuprous-catalyzed azide-alkyne cycloaddition (cuaac). the detailed structural characterization was investigated by means of ftir, uv-vis, (1)h nmr, and (13)c nmr spectra. the antifungal activities of starch derivatives against colletotrichum lagenarium, fusarium oxysporum, and watermelon fusarium, were then assayed by hypha ... | 2017 | 28115090 |
| a tomatinase-like enzyme acts as a virulence factor in the wheat pathogen fusarium graminearum. | during their interactions with plants, fungal pathogens employ large numbers of pathogenesis-associated molecules including secreted effectors and enzymes that can degrade various defence compounds. however, in many cases, in planta targets of pathogen-produced enzymes remain unknown. we identified a gene in the wheat pathogen fusarium graminearum, encoding a putative enzyme that shows 84% sequence identity to fotom1, a tomatinase produced by the tomato pathogen fusarium oxysporum f. sp. lycoper ... | 2017 | 28109774 |
| structure-activity relationship of α mating pheromone from the fungal pathogen fusarium oxysporum. | during sexual development ascomycete fungi produce two types of peptide pheromones termed a and α. the α pheromone from the budding yeast saccharomyces cerevisiae, a 13-residue peptide that elicits cell cycle arrest and chemotropic growth, has served as paradigm for the interaction of small peptides with their cognate g protein-coupled receptors. however, no structural information is currently available for α pheromones from filamentous ascomycetes, which are significantly shorter and share almo ... | 2017 | 28100777 |
| fusaric acid contributes to virulence of fusarium oxysporum on plant and mammalian hosts. | fusaric acid (fa) is amongst the oldest identified secondary metabolites produced by fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of fa and its function in fungal pathogenicity remain unknown. here, we investigated the role of fa in fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. targeted deletion ... | 2017 | 28093838 |
| comparative transcriptomics atlases reveals different gene expression pattern related to fusarium wilt disease resistance and susceptibility in two vernicia species. | vernicia fordii (tung oil tree) is a promising industrial crop. unfortunately, the devastating fusarium wilt disease has caused its great losses, while its sister species (vernicia montana) is remarkably resistant to this pathogen. however, the genetic mechanisms underlying this difference remain largely unknown. we here generated comparative transcriptomic atlases for different stages of fusarium oxysporum infected vernicia root. the transcriptomes of v. fordii and v. montana were assembled de ... | 2016 | 28083008 |
| tricalcium phosphate solubilization and nitrogen fixation by newly isolated aneurinibacillus aneurinilyticus ckmv1 from rhizosphere of valeriana jatamansi and its growth promotional effect. | aneurinibacillus aneurinilyticus strain ckmv1 was isolated from rhizosphere of valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/l), nitrogen fixation (202.91nmolethyleneml(-1)h(-1)), indole-3-acetic acid (iaa) (8.1μg/ml), siderophores (61.60%), hcn (hydrogen cyanide) production and antifungal activity. we investigated the ability of isolate ckmv1 to solubilize insoluble p via mechanism of organic acid production. high-per ... | 2017 | 28063921 |
| down-regulatory effect of thymus vulgaris l. on growth and tri4 gene expression in fusarium oxysporum strains. | the aims of this study were to evaluate the efficacy of thymus vulgaris (t. vulgaris) essential oil on the fungal growth and tri4 gene expression in fusarium oxysporum (f. oxysporum) strains. the oil was obtained by water-distillation using a clevenger-type system. the chemical composition of the essential oil was obtained by gas chromatography- mass spectroscopy (gc-ms) and by retention indices. the antifungal activity was evaluated by broth microdilution assay. a quantitative real time rt-pcr ... | 2017 | 28062283 |
| chemical profile and defensive function of the latex of euphorbia peplus. | plant latex is an endogenous fluid secreted from highly specialized laticifer cells and has been suggested to act as a plant defense system. the chemical profile of the latex of euphorbia peplus was investigated. a total of 13 terpenoids including two previously unknown diterpenoids, (2s*,3s*,4r*,5r*,6r*,8r*,l1r*,13s*,14s*,15r*, 16r*)-5,8,15-triacetoxy-3-benzoyloxy-11,16-dihydroxy-9-oxopepluane and (2r*,3r*, 4s*,5r*,7s*,8s*,9s*,l3s*,14s*,15r*)-2,5,8,9,14-pentaacetoxy-3-benzoyloxy-15-hydroxy-7-is ... | 2017 | 28062071 |
| a novel peroxidase purified from marsdenia megalantha latex inhibits phytopathogenic fungi mediated by cell membrane permeabilization. | an antifungal class iii peroxidase was purified from marsdenia megalantha latex (named mo-pox) using deae-cellulose and gel filtration chromatography on a superose 12 hr 10/30 column. mm-pox has an apparent molecular mass of 67.0kda and a pi of 5.2, shares identity with other peroxidases, and follows michaelis-menten kinetics. it has a high affinity for guaiacol and hydrogen peroxide. the ph and temperature optima for mm-pox were 5.0-7.0 and 60°c, respectively. the catalytic activity of mm-pox w ... | 2017 | 28057569 |
| defense peptide repertoire of stellaria media predicted by high throughput next generation sequencing. | being perfectly adapted to diverse environments, chickweed (stellaria media (l.) vill), a ubiquitous garden weed, grows widely in europe and north america. as opposed to the model plants, many weeds, and s. media in particular, have been poorly studied, although they are likely to contain promising components of immunity and novel resistance genes. in this study, for the first time rna-seq analysis of healthy and infected with fusarium oxysporum chickweed seedlings, as well as de novo transcript ... | 2017 | 28038935 |
| correction: transcription factors encoded on core and accessory chromosomes of fusarium oxysporum induce expression of effector genes. | [this corrects the article doi: 10.1371/journal.pgen.1006401.]. | 2016 | 28033317 |
| the tomato i gene for fusarium wilt resistance encodes an atypical leucine-rich repeat receptor-like protein whose function is nevertheless dependent on sobir1 and serk3/bak1. | we have identified the tomato i gene for resistance to the fusarium wilt fungus fusarium oxysporum f. sp. lycopersici (fol) and show that it encodes a membrane-anchored leucine-rich repeat receptor-like protein (lrr-rlp). unlike most other lrr-rlp genes involved in plant defence, the i gene is not a member of a gene cluster and contains introns in its coding sequence. the i gene encodes a loopout domain larger than those in most other lrr-rlps, with a distinct composition rich in serine and thre ... | 2017 | 27995670 |
| bioactive secondary metabolites with multiple activities from a fungal endophyte. | in order to replace particularly biohazardous nematocides, there is a strong drive to finding natural product-based alternatives with the aim of containing nematode pests in agriculture. the metabolites produced by the fungal endophyte fusarium oxysporum 162 when cultivated on rice media were isolated and their structures elucidated. eleven compounds were obtained, of which six were isolated from a fusarium spp. for the first time. the three most potent nematode-antagonistic compounds, 4-hydroxy ... | 2017 | 27990770 |
| non-canonical helitrons in fusarium oxysporum. | helitrons are eukaryotic rolling circle transposable elements that can have a large impact on host genomes due to their copy-number and their ability to capture and copy genes and regulatory elements. they occur widely in plants and animals, and have thus far been relatively little investigated in fungi. | 2016 | 27990178 |
| antibacterial combination of oleoresin from copaifera multijuga hayne and biogenic silver nanoparticles towards streptococcus agalactiae. | streptococcus agalactiae (group b streptococcus - gbs) remains a leading cause of neonatal infections and an important cause of invasive infections in adults with underlying conditions. | 2016 | 27978809 |
| controlled biosynthesis of silver nanoparticles using nitrate reductase enzyme induction of filamentous fungus and their antibacterial evaluation. | the controlled synthesis of silver nanoparticles (agnps) using cell-free filtrate of fusarium oxysporum fungus was investigated. the effect of fungal incubation period on nanoparticle formation and nitrate reductase enzyme activity was studied using uv-visible spectroscopy and harley assay, respectively. the highest agnp formation was observed in the cell-free filtrate of biomass harvested at the early stationary phase where the nr enzyme activity is the maximum. mixing of the cell-free filtrate ... | 2016 | 27966375 |
| stilbene derivatives from photorhabdus temperata sn259 and their antifungal activities against phytopathogenic fungi. | chemical investigation of an insect pathogenic enterobacterium, photorhabdus temperata sn259, led to the isolation and identification of seven metabolites, which include three new compounds, 3-hydroxy-2-isopropyl-5-phenethylphenyl carbamate, 1, 2-(1-hydroxypropan-2-yl)-5-[2-phenylethyl]benzene-1,3-diol, 2, 2-(1-hydroxypropan-2-yl)-5-[(e)-2-phenylethenyl]benzene-1,3-diol, 3, and four known metabolites (4-7). their structures were elucidated on the basis of ms and nmr data and by comparison with t ... | 2017 | 27960253 |
| effects of iron and boron combinations on the suppression of fusarium wilt in banana. | the effects of mineral nutrient on banana wilt disease, which are the result of a competitive relationship between host plants and pathogens, can affect the interactions of plants with microorganisms. to investigate the mineral nutrient effect, hydroponic experiments were conducted in glasshouse containing combinations of low, medium, and high iron (fe) and boron (b) concentrations, followed by pathogen inoculation. high fe and b treatment significantly reduced the disease index and facilitated ... | 2016 | 27941854 |
| recombinant expression, characterization, and application of a phospholipase b from fusarium oxysporum. | in this study, a gene encoding a putative lipase from fusarium oxysporum was optimized via codon optimization and expressed in pichia pastoris km71. the gene product was identified as a phospholipase b (plb). the engineered p. pastoris was further cultured in a 3.6-l bioreactor. after optimization of the induction conditions, this system produced 6.6mgml(-1) protein and 6503.8uml(-1) plb activity in the culture medium. efficient expression of this plb in p. pastoris should reduce the costs of pr ... | 2017 | 27940286 |
| races of the celery pathogen fusarium oxysporum f. sp. apii are polyphyletic. | fusarium oxysporum species complex (fosc) isolates were obtained from celery with symptoms of fusarium yellows between 1993 and 2013 primarily in california. virulence tests and a two-gene dataset from 174 isolates indicated that virulent isolates collected before 2013 were a highly clonal population of f. oxysporum f. sp. apii race 2. in 2013, new highly virulent clonal isolates, designated race 4, were discovered in production fields in camarillo, california. long-read illumina data were used ... | 2017 | 27938244 |
| proliferation of the biocontrol agent fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies. | our objectives were to (1) monitor the proliferation of the biocontrol agent (bca) fusarium oxysporum f. sp. strigae strain "foxy-2", an effective soil-borne bca against the parasitic weed striga hermonthica, in the rhizosphere of maize under different agro-ecologies, and (2) investigate its impact on indigenous rhizosphere fungal community abundance and composition. field experiments were conducted in busia and homa bay districts in western kenya during two cropping seasons to account for effec ... | 2016 | 27928553 |
| sequencing of individual chromosomes of plant pathogenic fusarium oxysporum. | a small chromosome in reference isolate 4287 of f. oxysporum f. sp. lycopersici (fol) has been designated as a 'pathogenicity chromosome' because it carries several pathogenicity related genes such as the secreted in xylem (six) genes. sequence assembly of small chromosomes in other isolates, based on a reference genome template, is difficult because of karyotype variation among isolates and a high number of sequences associated with transposable elements. these factors often result in misassemb ... | 2017 | 27919652 |
| divergent expression patterns in two vernicia species revealed the potential role of the hub gene vmap2/erf036 in resistance to fusarium oxysporum in vernicia montana. | tung oil tree (vernicia fordii) is a promising industrial oil crop; however, this tree is highly susceptible to fusarium wilt disease. conversely, vernicia montana is resistant to the pathogen. the apetala2/ethylene-responsive element binding factor (ap2/erf) transcription factor superfamily has been reported to play a significant role in resistance to fusarium oxysporum. in this study, comprehensive analysis identified 75 and 81 putative vf/vmap2/erf transcription factor-encoding genes in v. fo ... | 2016 | 27916924 |
| multiple evolutionary trajectories have led to the emergence of races in fusarium oxysporum f. sp. lycopersici. | race 1 isolates of fusarium oxysporum f. sp. lycopersici (fol) are characterized by the presence of avr1 in their genomes. the product of this gene, avr1, triggers resistance in tomato cultivars carrying resistance gene i in fol race 2 and race 3 isolates, avr1 is absent, and hence they are virulent on tomato cultivars carrying i in this study, we analyzed an approximately 100-kb genomic fragment containing the avr1 locus of fol race 1 isolate 004 (fol004) and compared it to the sequenced genome ... | 2017 | 27913420 |
| insights into the regulation of rhizosphere bacterial communities by application of bio-organic fertilizer in pseudostellaria heterophylla monoculture regime. | the biomass and quality of pseudostellariae heterophylla suffers a significant decline under monoculture. since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qpcr was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. the results showed compared with the 1st-year planted (fp), 2nd-year monoculture of p. heterophylla (sp) led to a significant decline in yield and resulted in a s ... | 2016 | 27899917 |
| evaluation of corneal cross-linking for treatment of fungal keratitis: using confocal laser scanning microscopy on an ex vivo human corneal model. | some previous reports have established the use of photoactivated chromophore-induced corneal cross-linking (pack-cxl) in treating fungal keratitis. the results of these case reports have often been conflicting. to systematically study the effect of pack-cxl in the management of fusarium keratitis, we have developed an ex vivo model of human corneal infection using eye-banked human corneas. | 2016 | 27898982 |
| effects of polysaccharide elicitors from endophytic fusarium oxysporum fat9 on the growth, flavonoid accumulation and antioxidant property of fagopyrum tataricum sprout cultures. | the purpose of this study was to evaluate the effects of four different fungal polysaccharides, named water-extracted mycelia polysaccharide (wps), sodium hydroxide-extracted mycelia polysaccharide (sps), hydrochloric-extracted mycelia polysaccharide (aps), and exo-polysaccharide (eps) obtained from the endophytic fusarium oxysporum fat9 on the sprout growth, flavonoid accumulation, and antioxidant capacity of tartary buckwheat. without visible changes in the appearance of the sprouts, the exoge ... | 2016 | 27897983 |
| antifungal activities of selected essential oils against fusarium oxysporum f. sp. lycopersici 1322, with emphasis on syzygium aromaticum essential oil. | the antifungal effects of four essential oils viz., clove (syzygium aromaticum), lemongrass (cymbopogon citratus), mint (mentha × piperita) and eucalyptus (eucalyptus globulus) were evaluated against wilt causing fungus, fusarium oxysporum f. sp. lycopersici 1322. the inhibitory effect of oils showed dose-dependent activity on the tested fungus. most active being the clove oil, exhibiting complete inhibition of mycelial growth and spore germination at 125 ppm with ic50 value of 18.2 and 0.3 ppm, ... | 2017 | 27876218 |
| efficient continuous biosynthesis of silver nanoparticles by activated sludge micromycetes with enhanced tolerance to metal ion toxicity. | the method for producing agnps by granules of activated sludge micromycetes with enhanced tolerance to metal ion toxicity - penicillium glabrum, fusarium nivale and fusarium oxysporum has been developed; the optimum conditions for agnp biosynthesis being found: the ag(+) ion concentration, duration of the contact of microbial cells with silver ions, a growth phase of microorganisms, medium composition, a рн value, mixing conditions, and also lighting intensity. the effect of cl(-), so4(2-) and h ... | 2016 | 27866609 |
| heat shock transcription factors in banana: genome-wide characterization and expression profile analysis during development and stress response. | banana (musa acuminata) is one of the most popular fresh fruits. however, the rapid spread of fungal pathogen fusarium oxysporum f. sp. cubense (foc) in tropical areas severely affected banana growth and production. thus, it is very important to identify candidate genes involved in banana response to abiotic stress and pathogen infection, as well as the molecular mechanism and possible utilization for genetic breeding. heat stress transcription factors (hsfs) are widely known for their common in ... | 2016 | 27857174 |
| transcription factors encoded on core and accessory chromosomes of fusarium oxysporum induce expression of effector genes. | proteins secreted by pathogens during host colonization largely determine the outcome of pathogen-host interactions and are commonly called 'effectors'. in fungal plant pathogens, coordinated transcriptional up-regulation of effector genes is a key feature of pathogenesis and effectors are often encoded in genomic regions with distinct repeat content, histone code and rate of evolution. in the tomato pathogen fusarium oxysporum f. sp. lycopersici (fol), effector genes reside on one of four acces ... | 2016 | 27855160 |
| antifungal lipopeptides produced by bacillus sp. fjat-14262 isolated from rhizosphere soil of the medicinal plant anoectochilus roxburghii. | this study aimed to develop biocontrol bacillus and explore bacterial biocontrol substances. according to the blood agar test, strain fjat-14262 was screened as a biosurfactant-producer. the biosurfactant-producing ability of fjat-14262 was further confirmed by the oil spreading tests because of its amphipathic character. furthermore, its fermentation supernatant could decrease the surface tension from 74.1 to 32.7 mn m(-1). fourier transform infrared spectroscopy (ft-ir) analysis indicated that ... | 2016 | 27854037 |
| genome wide transcriptome profiling of fusarium oxysporum f sp. ciceris conidial germination reveals new insights into infection-related genes. | vascular wilt caused by fusarium oxysporum f. sp. ciceris (foc) is a serious disease of chickpea (cicer arietinum l.) accounting for approximately 10-15% annual crop loss. the fungus invades the plant via roots, colonizes the xylem vessels and prevents the upward translocation of water and nutrients. infection is initiated by conidia that invade the host tissue often by penetration of intact epidermal cells. here, we report the characterization of the transcriptome of foc sequenced using illumin ... | 2016 | 27853284 |
| transcriptome analysis of the fungal pathogen fusarium oxysporum f. sp. medicaginis during colonisation of resistant and susceptible medicago truncatula hosts identifies differential pathogenicity profiles and novel candidate effectors. | pathogenic members of the fusarium oxysporum species complex are responsible for vascular wilt disease on many important crops including legumes, where they can be one of the most destructive disease causing necrotrophic fungi. we previously developed a model legume-infecting pathosystem based on the reference legume medicago truncatula and a pathogenic f. oxysporum forma specialis (f. sp.) medicaginis (fom). to dissect the molecular pathogenicity arsenal used by this root-infecting pathogen, we ... | 2016 | 27809762 |
| a gbs-snp-based linkage map and quantitative trait loci (qtl) associated with resistance to fusarium oxysporum f. sp. niveum race 2 identified in citrullus lanatus var. citroides. | a major qtl for resistance to fusarium oxysporum f. sp. niveum race 2 was mapped to a narrow 1.2 mb interval using a high-density gbs-snp linkage map, the first map of citrullus lanatus var. citroides. fusarium wilt, a fungal disease caused by fusarium oxysporum f. sp. niveum (fon), devastates watermelon crop production worldwide. several races, which are differentiated by host range, of the pathogen exist. resistance to fon race 2, a particularly virulent strain prevalent in the united states, ... | 2017 | 27803951 |
| naphthalene acetic acid potassium salt (naa-k(+)) affects conidial germination, sporulation, mycelial growth, cell surface morphology, and viability of fusarium oxysporum f. sp. radici-lycopersici and f. oxysporum f. sp. cubense in vitro. | the response to exogenous addition of naphthalene acetic acid potassium salt (naa-k(+)) to fusarium oxysporum f. sp radici-lycopersici atcc 60095 and f. oxysporum f. sp. cubense isolated from michoacan mexico soil is reported. the in vitro study showed that naa-k(+) might be effective in the control of fusarium oxysporum. exogenous application of naa-k(+) affected both spores and mycelium stages of the fungi. viability testing using acridine orange and propidium iodide showed that naa-k(+) posse ... | 2016 | 27754669 |
| koninginins r-s from the endophytic fungus trichoderma koningiopsis. | two new metabolites named koninginins r-s (1-2) were isolated from the culture of trichoderma koningiopsis yim ph30002. their chemical structures were elucidated by the extensive spectroscopic analysis. these isolated compounds showed certain antifungal activities against phytopathogens, fusarium flocciferum and fusarium oxysporum. | 2017 | 27784167 |
| photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi fusarium oxysporum, fusarium moniliforme and fusarium solani. | the search for alternatives to control microorganisms is necessary both in clinical and agricultural areas. antimicrobial photodynamic treatment (apdt) is a promising light-based approach that can be used to control both human and plant pathogenic fungi. in the present study, we evaluated the effects of photodynamic treatment with red light and four phenothiazinium photosensitizers (ps): methylene blue (mb), toluidine blue o (tbo), new methylene blue n (nmbn) and the phenothiazinium derivative s ... | 2016 | 27623154 |
| preparation, characterization, and bio-efficacy evaluation of controlled release carbendazim-loaded polymeric nanoparticles. | synthesis and controlled release study of polymeric nanoformulation of carbendazim (2-benzimidazole carbamic acid methyl ester) using chitosan and pectin is reported in this article. the formulation was subjected to morphological, physiological, in vitro fungicide release and bio-efficacy evaluation studies. the average size of nanoparticles was found to be in the range of 70-90 nm as confirmed by transmission electron microscopy. the in vitro fungicide release of nanoformulated carbendazim was ... | 2017 | 27761863 |
| identification and evidence of positive selection upon resistance gene analogs in cotton (gossypium hirsutum l.). | upland cotton (gossypium hirsutum l.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as fusarium oxysporum f. sp. vasinfectum (fov) causing severe wilt disease. resistance gene analogs (rgas) are the largest class of potential resistance (r) genes depicting highly conserved domains and structures in plants. additionally, rgas are pivotal components of breeding projects towards host disease resistance, serving as useful functional mark ... | 2016 | 27729728 |
| synthesis and antifungal activity against fusarium oxysporum of some brassinin analogs derived from l-tryptophan: a dft/b3lyp study on the reaction mechanism. | an efficient methodology to obtain novel antifungal analogs of brassinin 1 is described. starting from l-tryptophan 2, n,n'-dialkylthiourea 4, 4-[(1h-indol-3-yl)methylene]-2-sulfanylidene-1,3-thiazolidin-5-one 5 and alkyl (2s)-3-(1h-indol-3-yl)-2-{[(alkylsulfanyl)carbonothioyl]amino}propanoate 6 type compounds were obtained as main products in different ratios depending on the reaction conditions via a tandem dithiocarbamate formation and michael addition reaction. in order to understand the dep ... | 2016 | 27727186 |
| biocontrol agent fusarium oxysporum f.sp. strigae has no adverse effect on indigenous total fungal communities and specific amf taxa in contrasting maize rhizospheres. | we studied the effects of fusarium oxysporum f.sp. strigae (fos), a soil-borne biocontrol agent (bca) against striga hermonthica, on total fungal and arbuscular mycorrhizal fungal (amf) taxa in rhizospheres of maize in both clayey and sandy soil. effects of fos-bca 'foxy-2' were evaluated against (1) s. hermonthica presence, and (2) organic fertilization with tithonia diversifolia residues at 14, 28 and 42 d after 'foxy-2' inoculation, via dna-based quantitative pcr and trflp fingerprinting. in ... | 2016 | 27721900 |
| enhancement of pectinase production by ultraviolet irradiation and diethyl sulfate mutagenesis of a fusarium oxysporum isolate. | fusarium oxysporum strain bm-201 was treated with ultraviolet (uv) radiation to obtain a high pectinase-producing strain. mutant uv-10-41 was obtained and then treated by diethyl sulfate. next, the mutant uv-diethyl sulfate-43 derived from uv-10-41 was selected as high pectinase-producing strain. mutant uv-diethyl sulfate-43 was incubated on slant for 10 generations, demonstrating that the pectinase-producing genes were stable. pectinase activity reached 391.2 u/ml, which is 73.6% higher than th ... | 2016 | 27706785 |
| ccr4-not complex subunit not2 plays critical roles in vegetative growth, conidiation and virulence in watermelon fusarium wilt pathogen fusarium oxysporum f. sp. niveum. | ccr4-not complex is a multifunctional regulator that plays important roles in multiple cellular processes in eukaryotes. in the present study, the biological function of fonnot2, a core subunit of the ccr4-not complex, was explored in fusarium oxysporum f. sp. niveum (fon), the causal agent of watermelon wilt disease. fonnot2 was expressed at higher levels in conidia and germinating conidia and during infection in fon-inoculated watermelon roots than in mycelia. targeted disruption of fonnot2 re ... | 2016 | 27695445 |
| enhancement of trichothecene mycotoxins of fusarium oxysporum by ferulic acid aggravates oxidative damage in rehmannia glutinosa libosch. | rehmannia glutinosa is an important medicinal herb that cannot be replanted in the same field due to the effects of autotoxic substances. the effects of these substances on r. glutinosa in continuous cropping systems are unknown. in the present study, bioassays revealed that r. glutinosa exhibited severe growth restriction and higher disease indices in the fo+fa (f.oxysporum pretreated with ferulic acid) treatment. the increases in the contents of mda and h2o2 were greater in the fa+fo treatment ... | 2016 | 27667444 |
| a clonal lineage of fusarium oxysporum circulates in the tap water of different french hospitals. | fusarium oxysporum is typically a soilborne fungus but can also be found in aquatic environments. in hospitals, water distribution systems may be reservoirs for the fungi responsible for nosocomial infections. f. oxysporum was previously detected in the water distribution systems of five french hospitals. sixty-eight isolates from water representative of all hospital units that were previously sampled and characterized by translation elongation factor 1α sequence typing were subjected to microsa ... | 2016 | 27663024 |
| synthesis and fungicidal activity of tryptophan analogues - the unexpected calycanthaceous alkaloid derivatives. | a series of 21 n-protected tryptophan derivatives were synthesised from tryptophan in good yields. their structures were characterised by ir, (1)h nmr, (13)c nmr, dept (90° and 135°) and ms analysis. the synthesised compounds were evaluated against a wide variety of plant pathogen fungi. compounds a19 and a21 displayed activity against fusarium oxysporum (f. oxysporum), and compound a21 showed high activity against f. oxysporum and eggplant verticillium, with ec50 values of 58.27 and 77.39 μg ml ... | 2017 | 27653454 |
| identification, transcriptional and functional analysis of heat-shock protein 90s in banana (musa acuminata l.) highlight their novel role in melatonin-mediated plant response to fusarium wilt. | as one popular fresh fruit, banana (musa acuminata) is cultivated in the world's subtropical and tropical areas. in recent years, pathogen fusarium oxysporum f. sp. cubense (foc) has been widely and rapidly spread to banana cultivated areas, causing substantial yield loss. however, the molecular mechanism of banana response to foc remains unclear, and functional identification of disease-related genes is also very limited. in this study, nine 90 kda heat-shock proteins (hsp90s) were genomewide i ... | 2017 | 27627033 |
| a fungal pathogen secretes plant alkalinizing peptides to increase infection. | plant infections caused by fungi are often associated with an increase in the ph of the surrounding host tissue(1). extracellular alkalinization is thought to contribute to fungal pathogenesis, but the underlying mechanisms are poorly understood. here, we show that the root-infecting fungus fusarium oxysporum uses a functional homologue of the plant regulatory peptide ralf (rapid alkalinization factor)(2,3) to induce alkalinization and cause disease in plants. an upshift in extracellular ph prom ... | 2016 | 27572834 |
| an evaluation method for the suppression of pathogenic fusarium oxysporum by soil microorganisms using the dilution plate technique. | soil-borne diseases caused by pathogenic microorganisms are one of the main factors responsible for the decline in crop yields in farmlands. pathogenic fusarium oxysporum causes serious damage to various crops, and, thus, a feasible diagnostic method for soil-borne diseases is required. we herein examined a simple method to evaluate the suppressiveness of soil microorganisms against a pathogen by co-cultivating indigenous soil microorganisms and a pathogenic fungus (f. oxysporum f. sp. spinaciae ... | 2016 | 27558588 |
| striga biocontrol on a toothpick: a readily deployable and inexpensive method for smallholder farmers. | striga hermonthica (witchweed) is a parasitic weed that attacks and significantly reduces the yields of maize, sorghum, millet, and sugarcane throughout sub-saharan africa. low cost management methods such as hand weeding, short crop rotations, trap cropping, or conventional biocontrol have not been effective. likewise, striga-tolerant or herbicide-resistant maize cultivars are higher yielding, but are often beyond the economic means of sustenance farmers. the fungal pathogen, fusarium oxysporum ... | 2016 | 27551284 |
| potential biocontrol actinobacteria: rhizospheric isolates from the argentine pampas lowlands legumes. | control of fungal plant diseases by using naturally occurring non-pathogenic microorganisms represents a promising approach to biocontrol agents. this study reports the isolation, characterization, and fungal antagonistic activity of actinobacteria from forage soils in the flooding pampa, argentina. a total of 32 saprophytic strains of actinobacteria were obtained by different isolation methods from rhizospheric soil of lotus tenuis growing in the salado river basin. based on physiological trait ... | 2016 | 27550574 |
| soil bacterial and fungal community dynamics in relation to panax notoginseng death rate in a continuous cropping system. | notoginseng (panax notoginseng), a valuable herbal medicine, has high death rates in continuous cropping systems. variation in the soil microbial community is considered the primary cause of notoginseng mortality, although the taxa responsible for crop failure remains unidentified. this study used high-throughput sequencing methods to characterize changes in the microbial community and screen microbial taxa related to the death rate. fungal diversity significantly decreased in soils cropped with ... | 2016 | 27549984 |
| suppressor of fusion, a fusarium oxysporum homolog of ndt80, is required for nutrient-dependent regulation of anastomosis. | heterokaryon formation is an essential step in asexual recombination in fusarium oxysporum. filamentous fungi have an elaborate nonself recognition machinery to prevent formation and proliferation of heterokaryotic cells, called heterokaryon incompatibility (hi). in f. oxysporum the regulation of this machinery is not well understood. in neurospora crassa, vib-1, a putative transcription factor of the p53-like ndt80 family of transcription factors, has been identified as global regulator of hi. ... | 2016 | 27531696 |
| two novel antimicrobial defensins from rice identified by gene coexpression network analyses. | defensins form an antimicrobial peptides (amp) family, and have been widely studied in various plants because of their considerable inhibitory functions. however, their roles in rice (oryza sativa l.) have not been characterized, even though rice is one of the most important staple crops that is susceptible to damaging infections. additionally, a previous study identified 598 rice genes encoding cysteine-rich peptides, suggesting there are several uncharacterized amps in rice. we performed in si ... | 2016 | 27527801 |
| chickpea ferritin cafer1 participates in oxidative stress response, and promotes growth and development. | ferritins store and sequester iron, and regulate iron homeostasis. the cdna for a stress-responsive phytoferritin, previously identified in the extracellular matrix (ecm) of chickpea (cicer arietinum), was cloned and designated cafer1. the cafer1 transcript was strongly induced in chickpea exposed to dehydration, hypersalinity and aba treatment. additionally, it has role in the defense against fusarium oxysporum infection. functional complementation of the yeast frataxin-deficient mutant, δyfh1, ... | 2016 | 27503257 |