| weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution. | several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ... | 2010 | 20525872 |
| influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments. | because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ... | 2010 | 20553043 |
| structural and kinetic studies on the ser101ala variant of choline oxidase: catalysis by compromise. | the oxidation of choline catalyzed by choline oxidase includes two reductive half-reactions where fad is reduced by the alcohol substrate and by an aldehyde intermediate transiently formed in the reaction. each reductive half-reaction is followed by an oxidative half-reaction where the reduced flavin is oxidized by oxygen. here, we have used mutagenesis to prepare the ser101ala mutant of choline oxidase and have investigated the impact of this mutation on the structural and kinetic properties of ... | 2010 | 20561507 |
| high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india. | assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar. | 2010 | 20586938 |
| investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods. | water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ... | 2010 | 20587384 |
| variability of sediment-contact tests in freshwater sediments with low-level anthropogenic contamination--determination of toxicity thresholds. | freshwater sediments with low levels of anthropogenic contamination and a broad range of geochemical properties were investigated using various sediment-contact tests in order to study the natural variability and to define toxicity thresholds for the various toxicity endpoints. tests were performed with bacteria (arthrobacter globiformis), yeast (saccharomyces cerevisiae), nematodes (caenorhabditis elegans), oligochaetes (lumbriculus variegatus), higher plants (myriophyllum aquaticum), and the e ... | 2010 | 20594629 |
| prevalence of the gene trzn and biogeographic patterns among atrazine-degrading bacteria isolated from 13 colombian agricultural soils. | the following study evaluated the diversity and biogeography of 83 new atrazine-degrading bacteria and the composition of their atrazine degradation genes. these strains were isolated from 13 agricultural soils and grouped according to rep-pcr genomic fingerprinting into 11 major clusters, which showed biogeographic patterns. three clusters (54 strains) belonged to the genus arthrobacter, seven clusters (28 strains) were similar to the genus nocardioides and only one strain was a gram-negative f ... | 2010 | 20597985 |
| study on the removal of indoor vocs using biotechnology. | with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ... | 2010 | 20598440 |
| chemical composition and antibacterial activity of essential oils of lantana camara, ageratum houstonianum and eupatorium adenophorum. | essential oils have applications in folk medicine, food preservation, and as feed additives. the essential oils of lantana camara linn. (verbenaceae), ageratum houstonianum mill. (asteraceae) and eupatorium adenophorum spreng. (asteraceae) were analyzed by gas chromatography-mass spectrometry (gcms). in l. camara oil, of the total identified (83.91%) volatile constituents, five constituents [3,7,11-trimethyl-1,6,10-dodecatriene (28.86%), beta-caryophyllene (12.28%), zingiberene (7.63%), gamma-cu ... | 2010 | 20645797 |
| reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film. | polyacrylonitrile film (pan) surfaces were modified with chemical polymerization of conductive polyaniline (pani) in the presence of potassium dichromate as an oxidizing agent. the conductive films were used for immobilization of uricase. the surface resistance of the conductive film in this work was found to be 0.97 kω/cm. the maximum amount of immobilized enzyme on conductive film containing 2.4% pani was about 216 μg/cm(2). the optimum ph for free and immobilized enzymes was observed at 7.0 a ... | 2011 | 20652599 |
| air-cathode microbial fuel cell array: a device for identifying and characterizing electrochemically active microbes. | microbial fuel cells (mfcs) have generated excitement in environmental and bioenergy communities due to their potential for coupling wastewater treatment with energy generation and powering diverse devices. the pursuit of strategies such as improving microbial cultivation practices and optimizing mfc devices has increased power generating capacities of mfcs. however, surprisingly few microbial species with electrochemical activity in mfcs have been identified because current devices do not suppo ... | 2011 | 20655725 |
| a novel replicative enzyme encoded by the linear arthrobacter plasmid pal1. | the soil bacterium arthrobacter nitroguajacolicus rü61a contains the linear plasmid pal1, which codes for the degradation of 2-methylquinoline. like other linear replicons of actinomycetes, pal1 is characterized by short terminal inverted-repeat sequences and terminal proteins (tppal1) covalently attached to its 5' ends. tppal1, encoded by the pal1.102 gene, interacts in vivo with the protein encoded by pal1.101. bioinformatic analysis of the pal1.101 protein, which comprises 1,707 amino acids, ... | 2010 | 20675469 |
| biodegradation of 4-chlorophenol by arthrobacter chlorophenolicus a6: effect of culture conditions and degradation kinetics. | among known microbial species, arthrobacter chlorophenolicus a6 has shown very good potential to treat phenolic wastewaters. in this study, the levels of various culture conditions, namely initial ph, agitation (rpm), temperature (°c), and inoculum age (h) were optimized to enhance 4-chlorophenol (4-cp) biodegradation and the culture specific growth rate. for optimization, central composite design of experiments followed by response surface methodology (rsm) was applied. results showed that amon ... | 2011 | 20676733 |
| isolation and characterization of an arthrobacter sp. strain hb-5 that transforms atrazine. | a bacterial strain (hb-5) capable of utilizing atrazine as sole carbon and nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. the isolate was identified as arthrobacter sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain exhibited faster atrazine degradation rates in atrazine-containing mineral media than the well-characterized atrazine-degrading bacteria pseudomonas sp. adp. ... | 2011 | 20686824 |
| characterization of a bacterial community from a northeast siberian seacoast permafrost sample. | a combination of culture-dependent and -independent techniques was used to characterize a bacterial community, examine cold adaptation of isocitrate lyase (icl) genes, and detect genes with important ecological functions in a permafrost sample from the bykovsky peninsula on the laptev sea coast of northeast siberia. according to the 16s rrna gene sequence, 47 of the cultured isolates were members of the phyla firmicutes, proteobacteria, and actinobacteria, with 85% of the isolates belonging to t ... | 2010 | 20695892 |
| engineering host cell lines to reduce terminal sialylation of secreted antibodies. | covalently-linked glycans on proteins have many functional roles, some of which are still not completely understood. antibodies have a very specific glycan modification in the fc region that is required for mediating immune effector functions. these fc glycans are typically highly heterogeneous in structure, and this heterogeneity is influenced by many factors, such as type of cellular host and rate of ab secretion. glycan heterogeneity can affect the fc-dependent activities of antibodies. it ha ... | 2010 | 20716959 |
| isolation and characterization of hexavalent chromium-reducing rhizospheric bacteria from a wetland. | scirpus americanus pers. occurs naturally in "san germán," a pond that serves as a receptor of industrial wastewater in guanajuato, méxico. this plant accumulates metals mainly in the root: concentrations (mg/kg) of cr, as, cd and se were 970, 49, 41, and 85 respectively. analysis of rhizosphere samples indicated bacterial population of 10(8) cfu g(-1) in media with 0.2 mm cr(vi) and 10 mm sodium gluconate. thirteen isolates were obtained and phylogenetic analyses (16s rrna) indicated they corre ... | 2010 | 20734910 |
| diversity of cold-active protease-producing bacteria from arctic terrestrial and marine environments revealed by enrichment culture. | a new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from svalbard, norway. the method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. zobell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. one to three typ ... | 2010 | 20799082 |
| isolation and characterization of a pseudomonas sp. strain iitr01 capable of degrading α-endosulfan and endosulfan sulfate. | to isolate bacteria capable of degrading endosulfan (es) and the more toxic es sulfate and to characterize their metabolites. | 2010 | 20825519 |
| novel isonitrile hydratase involved in isonitrile metabolism. | we previously discovered n-substituted formamide deformylase (nfda) in arthrobacter pascens f164, which degrades n-substituted formamide (fukatsu, h., hashimoto, y., goda, m., higashibata, h., and kobayashi, m. (2004) proc. natl. acad. sci. u.s.a. 101, 13726-13731). in this study, we found an enzyme involved in the first step of isonitrile metabolism, isonitrile hydratase, that hydrates isonitrile to the corresponding n-substituted formamide. first, we investigated the optimum culture conditions ... | 2010 | 20826798 |
| phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal. | the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ... | 2010 | 20833430 |
| the catalytic efficiency of trehalose-6-phosphate synthase is effected by the n-loop at low temperatures. | the enzyme otsa (trehalose-6-phosphate synthase) is ubiquitous in both prokaryotic and eukaryotic organisms, where it plays a critical role in stress resistance and glucose metabolism. here, we cloned the otsa gene from arthrobacter sp. cjts, and expressed and then purified the recombinant proteins. enzyme activity analysis indicated that the high catalytic efficiency of otsa from arthrobacter sp. cjts resulted from the high affinity of the enzyme for uridine 5'-diphosphoglucose (udp-glc) at low ... | 2010 | 20838774 |
| arthrobacter equi sp. nov., a novel bacterium isolated from a veterinary clinical material. | a gram-positive, aerobic, catalase-positive, non-spore-forming, rod-shaped bacterium, strain immib l-1606t, isolated from genital swabs of a horse was characterized using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that the organism was related to the genus arthrobacter, displaying 93.5 - 99.1 % sequence similarities with established species of the genus. cell wall analysis revealed cell-wall peptidoglycan type a3α l-lys - l-ser - l-thr - l-ala. the results of dna-d ... | 2010 | 20870884 |
| [xylose isomerase constitutive biosynthesis in arthrobacter nicotianae]. | the specific features of biosynthesis of the cell-bound xylose isomerase by the actinobacterium arthrobacter nicotianae bim v-5 were studied. it was demonstrated that the constitutive synthesis of this enzyme in the studied bacteria, not subject to catabolite repression, was inhibited by xylulose, an intermediate product ofxylose utilization and the final product of its enzymatic isomerization. short-term experiments demonstrated that xylulose at a concentration of 0.005% almost completely repre ... | 2010 | 20873168 |
| bacterial community diversity in the brazilian atlantic forest soils. | the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ... | 2010 | 20886336 |
| identification of chitinases is-chia and is-chib from isoptericola jiangsuensis clg and their characterization. | a 274-bp conserved fragment of chia (chia-cf) was amplified from the genomic dna of isoptericola jiangsuensis clg (dsm 21863, cctcc ab208287) using the specific pcr primers. based on chia-cf sequences, a 5233-bp dna fragment was obtained by self-formed adaptor pcr. dna sequencing analysis revealed there were two contiguous open reading frames coding for the precursors of is-chia [871 amino acids (aa)] and is-chib (561 aa) in the 5233-bp dna fragment. the is-chia and is-chib exhibited 58% and 62% ... | 2010 | 20922373 |
| purification and characterization of inulin fructotransferase (dfa iii-forming) from arthrobacter aurescens sk 8.001. | the soil bacterium arthrobacter aurescens sk 8.001 produces inulin fructotransferase (iftase), and liquid chromatography-mass spectrometry (lc-ms) and carbon-13 nuclear magnetic resonance (13c nmr) analysis demonstrated that the main product of the enzyme was difructose anhydride iii (dfa iii). the iftase was purified by ethanol precipitation, deae sepharose fast flow, and superdex 200 10/300 gl gel chromatography. its molecular mass was estimated to be 40 kda by sds-page and 35 kda by gel filtr ... | 2010 | 20933390 |
| appraising bacterial strains for rapid bod sensing--an empirical test to identify bacterial strains capable of reliably predicting real effluent bods. | the measured response of rapid biochemical oxygen demand (bod) biosensors is often not identical to those measured using the conventional 5-day bod assay. this paper highlights the efficacy of using both glucose-glutamic acid (gga) and organisation for economic cooperation and development (oecd) bod standards as a rapid screen for microorganisms most likely to reliably predict real effluent bods when used in rapid bod devices. using these two synthetic bod standards, a microorganism was identifi ... | 2010 | 20938773 |
| diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania. | aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ... | 2010 | 20941581 |
| direct cellular lysis/protein extraction protocol for soil metaproteomics. | we present a novel direct protocol for deep proteome characterization of microorganisms in soil. the method employs thermally assisted detergent-based cellular lysis (sds) of soil samples, followed by tca precipitation for proteome extraction/cleanup prior to liquid chromatography-mass spectrometric characterization. this approach was developed and optimized using different soils inoculated with genome-sequenced bacteria (gram-negative pseudomonas putida or gram-positive arthrobacter chloropheno ... | 2010 | 20954746 |
| transgenic tobacco plants expressing atza exhibit resistance and strong ability to degrade atrazine. | atrazine chlorohydrolase (atza) catalyzes hydrolytic dechlorination and can be used in detoxification of atrazine, a herbicide widely employed in the control of broadleaf weeds. in this study, to investigate the potential use of transgenic tobacco plants for phytoremediation of atrazine, atza genes from pseudomonas sp. strain adp and arthrobacter strain ad1 were transferred into tobacco. three and four transgenic lines, expressing atza-adp and atza-ad1, respectively, were produced by agrobacteri ... | 2010 | 20960204 |
| 16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull. | marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ... | 2010 | 21038149 |
| transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity. | transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ... | 2010 | 21043054 |
| biodegradability of bacterial surfactants. | this work aimed at evaluating the biodegradability of different bacterial surfactants in liquid medium and in soil microcosms. the biodegradability of biosurfactants by pure and mixed bacterial cultures was evaluated through co(2) evolution. three bacterial strains, acinetobacter baumanni lbbma es11, acinetobacter haemolyticus lbbma 53 and pseudomonas sp. lbbma 101b, used the biosurfactants produced by bacillus sp. lbbma 111a (mixed lipopeptide), bacillus subtilis lbbma 155 (lipopeptide), flavob ... | 2010 | 21053055 |
| optimization of electroporation conditions for arthrobacter with plasmid part2. | a prerequisite for genetic studies of arthrobacter is a high efficiency transformation system that allows for dna transfer, transposon mutagenesis, and expression of specific genes. in this study, we develop a detailed electroporation method through a systematic examination of the factors involved in the entire electroporation process. key features of this procedure, including the addition of penicillin to cells during the early log phase of growth and the presence of 0.5m sorbitol in the electr ... | 2010 | 21078345 |
| evaluation of the ltq-orbitrap mass spectrometer for the analysis of polymerase chain reaction products. | we have investigated the potential and robustness of the off-line coupling of polymerase chain reaction (pcr) with electrospray ionization mass spectrometry (esi-ms), for further applications in the screening of single-nucleotide polymorphisms (snps). this was based on recently reported data demonstrating that anion-exchange solid-phase extraction was the most efficient technique for efficiently desalting pcr products, with a recovery of ∼70%. results showed that this purification approach effic ... | 2010 | 21080500 |
| root exudates modify bacterial diversity of phenanthrene degraders in pah-polluted soil but not phenanthrene degradation rates. | to determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (pah) contaminated soil is affected by the addition of plant root exudates, dna stable isotope probing (sip) was used. microcosms of soil with and without addition of ryegrass exudates and with ¹³c-labelled phenanthrene (phe) were monitored over 12 days. phe degradation was slightly delayed in the presence of added exudate after 4 days of incubation. after 12 days, 68% of added phe di ... | 2010 | 21087382 |
| complete biodegradation of 4-fluorocinnamic acid by a consortium comprising arthrobacter sp. strain g1 and ralstonia sp. strain h1. | a consortium of the newly isolated bacterial strains arthrobacter sp. strain g1 and ralstonia sp. strain h1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. strain g1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. in the presence of strain h1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. degradation of 4-fl ... | 2010 | 21097599 |
| lactulose biosynthesis by β-galactosidase from a newly isolated arthrobacter sp. | lactulose, a ketose disaccharide, is used in both pharmaceutical and food industries. this study was undertaken to screen and isolate potent β-galactosidase-producing bacteria and to evaluate their enzymatic production of lactulose. soil samples from fruit gardens were collected. one isolate designated las was identified whose cell extract could convert lactose and fructose into lactulose. the 16s rdna gene analysis of las revealed its phylogenetic relatedness to arthrobacter sp. the β-galactosi ... | 2010 | 21104424 |
| degradative potential of stenotrophomonas strain hpc383 having genes homologous to dmp operon. | a strain, stenotrophomonas hpc383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through hplc and gc analysis. culture hpc383 could degrade (%) various compounds (1 mm) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% c ... | 2010 | 21123060 |
| isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil. | in total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the yellow sea, near the city of incheon in the republic of korea. all of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (iaa), nitrogen fixation, phosphorus (p) and zinc (zn) solubilization, thiosulfate (s2o3) oxidation, the production of ammonia ... | 2010 | 21124065 |
| the arthrobacter arilaitensis re117 genome sequence reveals its genetic adaptation to the surface of cheese. | arthrobacter arilaitensis is one of the major bacterial species found at the surface of cheeses, especially in smear-ripened cheeses, where it contributes to the typical colour, flavour and texture properties of the final product. the a. arilaitensis re117 genome is composed of a 3,859,257 bp chromosome and two plasmids of 50,407 and 8,528 bp. the chromosome shares large regions of synteny with the chromosomes of three environmental arthrobacter strains for which genome sequences are available: ... | 2010 | 21124797 |
| the biodegradation pathway of triethylamine and its biodegradation by immobilized arthrobacter protophormiae cells. | a bacterial strain named r4 was isolated from a wastewater treatment pool containing triethylamine (tea) as the sole source of carbon and nitrogen. strain r4 was identified as arthrobacter protophormiae based on 16s rrna gene sequence analysis and morphological and physiological properties. the optimal ph, temperature and concentration of nacl for tea degradation by strain r4 were 7.0, 30°c and 0.5%, respectively. strain r4 could completely degrade 100 mg l(-1) tea to ammonia in 32 h, and could ... | 2010 | 21134717 |
| erythema caused by a localised skin infection with arthrobacter mysorens. | skin erythemas of unknown origin are a frequent reason for consulting the general practitioner or dermatologist. | 2010 | 21159172 |
| detection of the reaction intermediates catalyzed by a copper amine oxidase. | to reveal the chemical changes and geometry changes of active-site residues that cooperate with a reaction is important for understanding the functional mechanism of proteins. consecutive temporal analyses of enzyme structures have been performed during reactions to clarify structure-based reaction mechanisms. phenylethylamine oxidase from arthrobacter globiformis (agao) contains a copper ion and topaquinone (tpq(ox)). the catalytic reaction of agao catalyzes oxidative deaminations of phenylethy ... | 2010 | 21169693 |
| importance of a serine proximal to the c(4a) and n(5) flavin atoms for hydride transfer in choline oxidase. | choline oxidase catalyzes the flavin-dependent, two-step oxidation of choline to glycine betaine with the formation of an aldehyde intermediate. in the first oxidation reaction, the alcohol substrate is initially activated to its alkoxide via proton abstraction. the substrate is oxidized via transfer of a hydride from the alkoxide α-carbon to the n(5) atom of the enzyme-bound flavin. in the wild-type enzyme, proton and hydride transfers are mechanistically and kinetically uncoupled. in this stud ... | 2011 | 21174412 |
| bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of lonar, india. | phenol is an industrially important compound which has a wide range of applications. being highly soluble in water, it appears as the major pollutant in waste waters arising from both phenol manufacturing and from industrial units that utilise phenol. because of its toxicity, bioremediation of phenol is necessary. since some of the phenol-bearing industrial waste waters are alkaline in nature, use of alkaliphilic bacteria for bioremediation of phenol was investigated. alkaliphilic bacteria were ... | 1998 | 21182701 |
| cyanobacteria as agents for the control of pollution by pesticides and chlorinated organic compounds. | cyanobacteria are phototrophic aquatic micro-organisms that are found in a variety of environments, including polluted ones. fifteen strains of cyanobacteria that belong to three taxonomic groups are able to degrade lindane (λ-hexachlorocyclohexane, a recalcitrant pesticide). the initial degradation pathway has been studied in two filamentous nitrogen-fixing strains of anabaena sp. pcc 7120 and nostoc ellipsosporum. these cyanobacteria dechlorinated loindane first to pentachlorocyclohexene and t ... | 1998 | 21182708 |
| replication-involved genes of pal1, the linear plasmid of arthrobacter nitroguajacolicus rü61a--phylogenetic and transcriptional analysis. | the 113-kb pal1 is the only arthrobacter linear plasmid known; it has terminal inverted repeats and 5' covalently attached terminal proteins (tps). the latter and a telomere-associated protein (tap) are encoded by plasmid orfs 102 and 101, respectively. as for streptomyces linear replicons, in which both above proteins are instrumental in telomere patching, they are involved in pal1 replication as well. however, the alignment of actinobacterial taps and tps revealed that pal1 and the linear elem ... | 2010 | 21185858 |
| studies of dimethylglycine oxidase isoenzymes in arthrobacter globiformis cells. | glycine betaine (gb) could be used by arthrobacter globiformis cells as a sole carbon source. the cells took up this molecule in the low as well as in the high salinity medium. addition of gb to the mineral medium with high salt concentration revealed that gb was also used as an osmoprotectant. dimethylglycine oxidase (dmgo) was involved in the catabolism of gb. two genes for dmgo were detected in a cloned 26267 bp fragment of a. globiformis dna. the genes involved in the tetrahydrofolate-depend ... | 2010 | 21188587 |
| discovery and characterization of d-phenylserine deaminase from arthrobacter sp. tks1. | we discovered a d-phenylserine deaminase that catalyzed the pyridoxal 5'-phosphate (plp)-dependent deamination reaction from d-threo-phenylserine to phenylpyruvate in newly isolated arthrobacter sp. tks1. the enzyme was partially purified, and its n-terminal amino acid sequence was analyzed. based on the sequence information, the gene encoding the enzyme was identified and expressed in escherichia coli. the expressed protein was purified to homogeneity and characterized. the enzyme consisted of ... | 2010 | 21190106 |
| structural and enzyme activity studies demonstrate that aryl substituted 2,3-butadienamine analogs inactivate arthrobacter globiformis amine oxidase (agao) by chemical derivatization of the 2,4,5-trihydroxyphenylalanine quinone (tpq) cofactor. | copper amine oxidases (caos) are a family of redox active enzymes containing a 2,4,5-trihydroxyphenylalanine quinone (tpq) cofactor generated from post translational modification of an active site tyrosine residue. the arthrobacter globiformis amine oxidase (agao) has been widely used as a model to guide the design and development of selective inhibitors of caos. in this study, two aryl 2,3-butadienamine analogs, racemic 5-phenoxy-2,3-pentadienylamine (popda) and racemic 6-phenyl-2,3-hexadienyla ... | 2011 | 21215824 |
| assimilative and co-metabolic degradation of chloral hydrate by bacteria and their bioremediation potential. | although the bacterial degradation of chloral hydrate (ch) has been recognized for several decades, its degradation pathway by assimilation has not been demonstrated. in this paper, we report the isolation of the lf54 bacterial strain, which utilizes ch as its sole carbon and energy source. lf54 converted ch into trichloroethanol (tcaol), which was dehalogenated to dichloroethanol (dcaol), and co(2) was detected as the end product. another strain that we isolated, rs20, co-metabolized ch into tc ... | 2011 | 21220209 |
| [effects of nitrogen and phosphorus fertilizer on atrazine degradation and detoxification by degrading strain hb-5]. | an atrazine-degrading strain hb-5 was used as a bacteria for biodegradation. treatments of soil with nitrogen single, phosphate single and nitrogen phosphate together with hb-5 were carried out for degradation and eco-toxicity test; then, relationship between atrazine degradation rate and soil available nitrogen, available phosphorus were discussed. atrazine residues were determined by hplc; available nitrogen was determined with alkaline hydrolysis diffusion method; available phosphorus was det ... | 2010 | 21229764 |
| micrococcus lactis sp. nov., isolated from a dairy industry waste treatment. | a gram-positive, yellow pigmented, actinobacterial strain dw152t was isolated from a dairy industry effluent treatment plant. the 16s rrna gene sequence analysis indicated that strain dw152t exhibited low similarity with many validly published species belonging to genera micrococcus and arthrobacter. however, phenotypic properties including chemotaxonomic markers affiliated strain dw152t to the genus micrococcus. strain dw152t had ai-c15:0 and i-c15:0 as major cellular fatty acids, and mk-8(h2) ... | 2011 | 21239567 |
| identification of the catalytic residues of carboxylesterase from arthrobacter globiformis by diisopropyl fluorophosphate-labeling and site-directed mutagenesis. | the role of amino acid residues in the enzymatic activity of carboxylesterase from arthrobacter globiformis was analyzed by diisopropyl fluorophosphate (dfp) labeling and site-directed mutagenesis. the electrospray ionization mass spectrometric (esi-ms) analysis of the esterase, covalently labeled by dfp, showed stoichiometric incorporation of the inhibitor into the enzyme. the further comparison of endopeptidase-digested fragments between native and dfp-labeled esterase by fast atom bombardment ... | 2011 | 21266781 |
| [diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province]. | to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china. | 2010 | 21268889 |
| in vivo self-hydroxylation of an iron-substituted manganese-dependent extradiol cleaving catechol dioxygenase. | the homoprotocatechuate 2,3-dioxygenase from arthrobacter globiformis (mndd) catalyzes the oxidative ring cleavage reaction of its catechol substrate in an extradiol fashion. although this reactivity is more typically associated with non-heme iron enzymes, mndd exhibits an unusual specificity for manganese(ii). mndd is structurally very similar to the iron(ii)-dependent homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum (hpcd), and we have previously shown that both mndd and hpcd are ... | 2011 | 21279661 |
| homologous gene clusters of nicotine catabolism, including a new ?-amidase for a-ketoglutaramate, in species of three genera of gram-positive bacteria. | gram-positive soil bacteria arthrobacter nicotinovorans, nocardioides sp. js614 and rhodococcus opacus were shown to contain similarly organized clusters of homologous genes for nicotine catabolism. an uncharacterized gene of a predicted nitrilase within these gene clusters was cloned from a. nicotinovorans and biochemical data unexpectedly showed that the protein exhibited ?-amidase activity toward a-ketoglutaramate. structural modelling of the protein suggested the presence of the catalytic tr ... | 2011 | 21288482 |
| transformation of tomato with a bacterial coda gene enhances tolerance to salt and water stresses. | genetically engineered tomato (lycopersicon esculentum) with the ability to synthesize glycinebetaine was generated by introducing the coda gene encoding choline oxidase from arthrobacter globiformis. integration of the coda gene in transgenic tomato plants was verified by pcr analysis and dna blot hybridization. transgenic expression of gene was verified by rt-pcr analysis and rna blot hybridization. the coda-transgenic plants showed higher tolerance to salt stress during seed germination, and ... | 2011 | 21342716 |
| [isolation, identification and soil remediation of atrazine-degrading strain t3 ab1]. | to provide new atrazine-degrading strains for atrazine-polluted soil, we isolated the high-efficiency degradation bacterium from contaminated soil, identified with taxonomy, and studied the degrading characteristics and remediation capability of the strain in black soil. | 2010 | 21365918 |
| crystallographic snapshots of the complete reaction cycle of nicotine degradation by an amine oxidase of the monoamine oxidase (mao) family. | fad-linked oxidases constitute a class of enzymes which catalyze dehydrogenation as a fundamental biochemical reaction, followed by reoxidation of reduced flavin. here, we present high-resolution crystal structures showing the flavoenzyme 6-hydroxy-l-nicotine oxidase in action. this enzyme was trapped during catalytic degradation of the native substrate in a sequence of discrete reaction states corresponding to the substrate-reduced enzyme, a complex of the enzyme with the intermediate enamine p ... | 2011 | 21383134 |
| isolation and characterization of metal resistant-tolerant rhizosphere bacteria from the serpentine soils in turkey. | despite the number of studies describing metal hyper-accumulating plants and their associated bacteria in various regions and countries, there is no information on rhizosphere microbial potential of the turkish serpentine soils. this study aimed to explore the rhizosphere microbial diversity of ni-resistant, hyper-accumulating plants grown on ni-rich soils and their metal tolerance-resistance characteristics. one hundred ninety-one locations were visited to collect soil and plant samples from di ... | 2011 | 21404012 |
| simultaneous phenanthrene and cadmium removal from contaminated soil by a ligand/biosurfactant solution. | surfactants and inorganic ligands are pointed as efficient to simultaneous removal of heavy metals and hydrophobic organic pollutants from soil. however, the biosurfactants are potentially less toxic to soil organisms than other chemical agents. thus, in this study the efficiency of combinations of iodide (i(-)) ligand and surfactants produced by different bacterial species in the simultaneous removal of cadmium (cd(2+)) and phenanthrene in a haplustox soil sample was investigated. four microbia ... | 2011 | 21416334 |
| genomic heterogeneity within conservedmetabolic pathways of arthrobacter species - a bioinformatic approach. | a comparative genomic analysis of three species of the soil bacterium arthrobacter was undertaken with specific emphasis on genes involved in important and core energy metabolism pathways like glycolysis and amino acid metabolism. during the course of this study, it was revealed that codon bias of a particular species, namely arthrobacter aurescens tc1, is significantly lower than that of the other two species a. chlorophenolicus a6 and arthrobacter sp. fb24. the codon bias was also found to be ... | 2011 | 21423891 |
| 2,4-d metabolism: pathway of degradation of chlorocatechols by arthrobacter sp. | | 1969 | 21428341 |
| novel bacterial strains for the removal of microcystins from drinking water. | microcystins (mc) and nodularin (nod) are common contaminants of drinking water around the world and due to their significant health impact it is important to explore suitable approaches for their removal. unfortunately, these toxins are not always removed by conventional water treatments. one of the most exciting areas that hold promise for a successful and cost effective solution is bioremediation of microcystins. recent work resulted in successful isolation and characterisation of 10 novel ba ... | 2011 | 21436548 |
| arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. | a gram-stain-positive, aerobic, non-motile and psychrophilic bacterium, designated strain cr6-08(t), was isolated from alpine glacier cryoconite. growth occurred at a temperature range of 1-25 °c. phylogenetic analyses based on 16s rrna gene sequences revealed that strain cr6-08(t) is most closely related to members of the genus arthrobacter. strain cr6-08(t) possessed chemotaxonomic properties indicative of members of the genus arthrobacter, such as peptidoglycan type a3a l-lys-l-ala4, major me ... | 2011 | 21441372 |
| molecular characterization of cold-inducible beta-galactosidase from arthrobacter sp. on14 isolated from antarctica. | a psychrotrophic bacterium, arthrobacter sp. on14, isolated from antarctica, was shown to exhibit a high beta-galactosidase activity at a low temperature. a genomic library of on14 was constructed and screened for beta-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal) as the substrate. two different beta-galactosidase genes, named as gala, galb, were found in on14. computational analyses of the genes revealed that the encoded protein ... | 2011 | 21464592 |
| effects of hydroxypropyl-β-cyclodextrin on cell growth, activity, and integrity of steroid-transforming arthrobacter simplex and mycobacterium sp. | a comparative investigation was performed on the effects of hydroxypropyl-β-cyclodextrin (hp-β-cd) on the growth, biocatalytic activity, and cell integrity of arthrobacter simplex tccc 11037 (asp) and mycobacterium sp. nrrl b-3683 (msp). the addition of hp-β-cd to asp medium improved its cell wall permeability for lipophilic compounds but significantly inhibited its growth and biocatalytic activity. on the other hand, the addition of hp-β-cd to msp broth had lesser effects. atomic force microsco ... | 2011 | 21468712 |
| biodegradation of p-nitrophenol using arthrobacter chlorophenolicus a6 in a novel upflow packed bed reactor. | a novel packed bed reactor (pbr) was designed with cross flow aeration at multiple ports along the depth to improve the hydrodynamic conditions of the reactor, and the biodegradation efficiency of arthrobacter chlorophenolicus a6 on p-nitrophenol (pnp) removal in pbr at different pnp loading rates were evaluated. the novel pbr was designed to improve the hydrodynamic features such as mixing time profile (t(m95)), oxygen mass transfer coefficient (k(l)a), and overall gas hold up capacity (ɛ(g)) o ... | 2011 | 21501928 |
| culturable bacterial diversity at the princess elisabeth station (utsteinen, sør rondane mountains, east antarctica) harbours many new taxa. | we studied the culturable heterotrophic bacterial diversity present at the site of the new princess elisabeth station at utsteinen (dronning maud land, east antarctica) before construction. about 800 isolates were picked from two terrestrial microbial mat samples after incubation on several growth media at different temperatures. they were grouped using rep-pcr fingerprinting and partial 16s rrna gene sequencing. phylogenetic analysis of the complete 16s rrna gene sequences of 93 representatives ... | 2011 | 21501941 |
| production of l-arabinose from corn hull arabinoxylan by arthrobacter aurescens mk5 α-l-arabinofuranosidase. | arabinoxylans, which are comprised of a xylan backbone to which are attached glycosyl units that are primarily l-arabinofuranosyl units, are ubiquitous among plant species where it is a constituent of the cell wall. arabinoxylan has attracted much attention as a potential biomass resource and l-arabinose has recently been reported to possess functional properties that are effective in the treatment of diabetes. here, we report an α-l-arabinofuranohydrolase, isolated from the soil microbe arthrob ... | 2011 | 21535740 |
| isolation and characterization of a family vii esterase derived from alluvial soil metagenomic library. | a novel esterase gene, estdl30, was isolated from an alluvial metagenomic library using function-driven screening. estdl30 consisted of 1,524 nucleotides and encoded a 507-amino acid protein. sequence analysis revealed that estdl30 is similar to many type b carboxylesterases, containing a g-e-s-a-g pentapeptide with a catalytic ser residue. phylogenetic analysis suggested that estdl30 belongs to the family vii lipases, together with esterases from bacillus subtilis (p37967), streptomyces coelico ... | 2011 | 21538236 |
| effect of zn(ii) on the reduction and accumulation of cr(vi) by arthrobacter species. | natural habitats are often characterized by the coexistence of zn and cr. this study assessed the potential of two gram-positive, cr(vi)-reducing, aerobic bacterial strains belonging to arthrobacter genera, which were isolated from basalt samples taken from the most polluted region of the republic of georgia, to remediate cr(vi) in environments in the presence of zn(ii). our batch experiments revealed that the addition of zn(ii) to the tested bacterial cells significantly enhanced the accumulati ... | 2011 | 21547698 |
| enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from arthrobacter sp. | in human serum, as for phospholipids not containing choline, phosphatidylethanolamine (pe) exists approximately 5% in a whole phospholipid. pe is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. however, it could not measure pe with other phospholipids due to a lack of choline in them. | 2011 | 21549106 |
| description of sinomonas soli sp. nov., reclassification of arthrobacter echigonensis and arthrobacter albidus (ding et al. 2009) as sinomonas echigonense comb. nov. and sinomonas albida comb. nov. and emended description of the genus sinomonas. | a novel actinomycete strain, designated cw 59(t), was isolated from a polluted forest soil sample in anhui province, china. the cells were strictly aerobic, non-motile and bent rods. the strain grew optimally at 30-37 °c and ph 6.0-8.0. the major fatty acids were ai-c(15:0) (34.7%), i-c(15:0) (11.6%) and ai-c(17:0) (14.9%); the predominant respiratory quinone was mk-9 (h2) with a minor amount of mk-8 (h2). the polar lipid composition of the strain cw 59(t) consisted of diphosphatidylglycerol, ph ... | 2011 | 21571934 |
| responses of bioaugmented ryegrass to pah soil contamination. | the physiological and biochemical responses of ryegrass (lolium multiflorum) to pah induced stress in soils contaminated with phenanthrene and pyene were investigated, in the presence of pah-degrading bacteria (acinetobacteria junii) or arbuscular mycorrhizal fungi (am fungi, glomus mossae). the parameters monitored included chlorophyll content, chlorophyll a/b ratio, soluble-carbohydrate content, soluble-protein, malondialdehyde and electrolyte leakage, and superoxide dismutase (sod) and peroxi ... | 2011 | 21598775 |
| the potential of established turf cover for cleaning oily desert soil using rhizosphere technology. | the rhizosphere of two turf cover sorts; bermuda grass and american grass contained high numbers, 8.1 to 16.8 x 10(6) g(-1) of cultivable oil-utilizing and diazotrophic bacteria belonging predominantly to the genera agrobacterium, arthrobacter, pseudomonas, gordonia, and rhodococcus. those bacteria also grew on a nitrogen-free medium and demonstrated the ability to reduce acetylene to ethylene. these isolates grew on a wide range of n-alkanes (c9 to c40) and aromatic hydrocarbons, as sole source ... | 2011 | 21598783 |
| growth-inhibition of t47d human breast-cancer cells by the new antiaromatase steroid 3-methylene-1,4-androstadien-17-one. | the c19 steroid, 3-methylene-1,4-androstadien-17-one (3-methylene-1-dehydro-4-androsten-17-one, abbrev. 3mda) was synthesized by microbial dehydrogenation with arthrobacter simplex from the corresponding 1,2-saturated, 3-methylene-4-androsten-17-one. the new steroid induces a type-1 optical difference spectrum with cytochrome p450 aromatase from human placentae (k(s) 1.61 mum). kinetic inhibition studies with placental microsomes show: (i) 3mda inhibits aromatase irreversibly (k(inact) 0.026 min ... | 1993 | 21573449 |
| regulation of expression of trehalose-6-phosphate synthase during cold shock in arthrobacter strain a3. | trehalose is a chemical chaperone known to protect a variety of organisms against cold stress. members of the genus arthrobacter, which belongs to the actinomycetales group, exhibit strong resistance to stress conditions, but exactly how trehalose synthesis is regulated in conditions of cold stress is still unknown. here, we report that arthrobacter strain a3, which was isolated from the alpine permafrost, has only two trehalose synthesis pathways (otsa/b and tres), while other arthrobacter spp. ... | 2011 | 21630027 |
| screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey. | several microorganisms were isolated from soil/sediment samples of antarctic peninsula. the enrichment technique using (rs)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. we also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (rs)-1-(phenyl)ethanol as substrates. among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze th ... | 2011 | 21673897 |
| characterisation of the staphylococcal bacteriophage lysin chap(k). | aims: to develop an efficient purification strategy for the bacteriophage lysin chap(k) . to evaluate its antibacterial spectrum(,) enzymatic properties, optimal reaction conditions and lytic activity against live s. aureus. methods and results: recombinant chap(k) was purified to homogeneity by cation exchange chromatography, with yields of up to 10 mg from 1 litre of e. coli culture. the lytic spectrum of chap(k) includes all staphylococcal species and also members of the genera micrcococcus ... | 2011 | 21812876 |
| phylogenetic perspectives of nitrogen-fixing actinobacteria. | it was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ... | 2011 | 21779790 |
| ecotoxicity evaluation of selected sulfonamides. | sulfonamides (sas) are a group of antibiotic drugs widely used in veterinary medicine. the contamination of the environment by these pharmaceuticals has raised concern in recent years. however, knowledge of their (eco)toxicity is still very basic and is restricted to just a few of these substances. even though their toxicological analysis has been thoroughly performed and ecotoxicological data are available in the literature, a systematic analysis of their ecotoxicological potential has yet to b ... | 2011 | 21752420 |
| effects of microbial species, organic loading and substrate degradation rate on the power generation capability of microbial fuel cells. | four microbial fuel cells (mfcs) inoculated with different bacterial species were constructed. the species were pseudomonas putida, comamonas testosteroni, corynebacterium gultamicum, and arthrobacter polychromogenes. the mfcs were operated under identical continuous flow conditions. the factors affecting the capabilities of the mfcs for treating organic matter and generating power were evaluated and compared. the factors include microbial species type, organic loading, and substrate degradation ... | 2011 | 21750995 |
| reutilization of cr-y zeolite obtained by biosorption in the catalytic oxidation of volatile organic compounds. | this work aims at the reutilization of a cr-loaded nay zeolite obtained by biorecovery of chromium from water as catalyst in the oxidation of volatile organic compounds (voc). cr-nay catalysts were obtained after biosorption of cr(vi) using a bacterium, arthrobacter viscosus, supported on the zeolite. the biosorption experiments were conducted at different ph values in the range 1-4. the catalysts were characterized by several techniques, namely icp-aes, sem-eds, xrd, xps, raman, h(2)-tpr and n( ... | 2011 | 21684081 |
| amylolytic microorganism from s+úo paulo zoo composting: isolation, identification, and amylase production. | composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. this transformation is the result of the microorganism action, converting complex carbon sources into energy. enzymes that are exported by the microorganisms to the surrounding environment mediate this process. the aiming of the present work is to prospect the compost produced by the organic composting unit (ocu) of the funda+º+úo parque zool+¦gic ... | 2011 | 21845217 |
| pa0148 from pseudomonas aeruginosa catalyzes the deamination of adenine. | four proteins from ncbi cog1816, previously annotated as adenosine deaminases, have been subjected to structural and functional characterization. pa0148 (pseudomonas aeruginosa pao1), aaur1117 (arthrobacter aurescens tc1), sgx9403e, and sgx9403g have been purified and their substrate profiles determined. adenosine is not a substrate for any of these enzymes. all of these proteins will deaminate adenine to produce hypoxanthine with k(cat)/k(m) values that exceed 10(5) m(-1) s(-1). these enzymes w ... | 2011 | 21710971 |
| bacterial diversity from benthic mats of antarctic lakes as a source of new bioactive metabolites. | during the micromat project, the bacterial diversity of microbial mats growing in the benthic environment of antarctic lakes was accessed for the discovery of novel antibiotics. in all, 723 antarctic heterotrophic bacteria belonging to novel and/or endemic taxa in the +¦-, +¦- and +¦-subclasses of the proteobacteria, the bacteroidetes branch, and of the high and low percentage g+c gram-positives, were isolated, cultivated in different media and at different temperatures, and then screened for th ... | 2009 | 21798170 |
| glycinebetaine enhances the tolerance of tomato plants to high temperature during germination of seeds and growth of seedlings. | tomato (lycopersicon esculentum cv. 'moneymaker') was transformed with a coda gene, from arthrobacter globiformis, for choline oxidase that had been modified to allow targeting to both chloroplasts and the cytosol. glycinebetaine (gb) accumulated in seeds of transformed plants up to 1 -ámol g(-1) dw, while no detectable gb was found in wild-type seeds. the coda-transgenic seeds germinated faster and at higher frequency than wild-type seeds with high temperature treatment. after heat stress, leve ... | 2011 | 21711358 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| biodegradation kinetics of 4-fluorocinnamic acid by a consortium of arthrobacter and ralstonia strains. | arthrobacter sp. strain g1 is able to grow on 4-fluorocinnamic acid (4-fca) as sole carbon source. the organism converts 4-fca into 4-fluorobenzoic acid (4-fba) and utilizes the two-carbon side-chain for growth with some formation of 4-fluoroacetophenone as a dead-end side product. we also have isolated ralstonia sp. strain h1, an organism that degrades 4-fba. a consortium of strains g1 and h1 degraded 4-fca with monod kinetics during growth in batch and continuous cultures. specific growth rate ... | 2011 | 21728015 |
| surface microbial consortia from livarot, a french smear-ripened cheese. | the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ... | 2011 | 21815832 |
| cultured bacterial diversity and human impact on alpine glacier cryoconite. | the anthropogenic effect on the microbial communities in alpine glacier cryoconites was investigated by cultivation and physiological characterization of bacteria from six cryoconite samples taken at sites with different amounts of human impact. two hundred and forty seven bacterial isolates were included in actinobacteria (9%, particularly arthrobacter), bacteroidetes (14%, particularly olleya), firmicutes (0.8%), alphaproteobacteria (2%), betaproteobacteria (16%, particularly janthinobacterium ... | 2011 | 21717318 |
| exposure to bioaerosols in the selected agricultural facilities of the ukraine and poland - a review. | the aim of this work was to review the studies on bioaerosols which were carried out in the years 1972-2009 in following branches of agricultural industry in the ukraine and poland: animal farms for cows and pigs, animal feed facilities, production of biofuel from rape, herb farms and herb processing facilities. in all facilities were determined: concentration of dust and microorganisms in the air and species composition of microflora. moreover, in polish animal farms, herb farms and herb proces ... | 2011 | 21736265 |
| molinate hydrolase from gulosibacter molinativorax on4t - a novel cobalt dependent amidohydrolase. | a new pathway of molinate mineralization has been recently described. among the five members of the mixed culture able to promote such a process, gulosibacter molinativorax on4(t) has been observed to promote the initial breakdown of the herbicide into ethanethiol and azepane-1-carboxylate. in the current study, the gene encoding the enzyme responsible for molinate hydrolysis was identified, heterologously expressed and the resultant active protein purified and characterized. nucleotide sequence ... | 2011 | 21840982 |
| sponge-associated microbial antarctic communities exhibiting antimicrobial activity against burkholderia cepacia complex bacteria. | the aerobic heterotrophic bacterial communities isolated from three different antarctic sponge species were analyzed for their ability to produce antimicrobial compounds active toward cystic fibrosis opportunistic pathogens belonging to the burkholderia cepacia complex (bcc). the phylogenetic analysis performed on the 16s rrna genes affiliated the 140 bacterial strains analyzed to 15 genera. just three of them (psychrobacter, pseudoalteromonas and arthrobacter) were shared by the three sponges. ... | 2011 | 21742025 |
| complete genome sequence of arthrobacter phenanthrenivorans type strain (sphe3). | arthrobacter phenanthrenivorans is the type species of the genus, and is able to metabolize phenanthrene as a sole source of carbon and energy. a. phenanthrenivorans is an aerobic, non-motile, and gram-positive bacterium, exhibiting a rod-coccus growth cycle which was originally isolated from a creosote polluted site in epirus, greece. here we describe the features of this organism, together with the complete genome sequence, and annotation. | 2011 | 21677849 |
| Kinetics of biodegradation of diethylketone by Arthrobacter viscosus. | The performance of an Arthrobacter viscosus culture to remove diethylketone from aqueous solutions was evaluated. The effect of initial concentration of diethylketone on the growth of the bacteria was evaluated for the range of concentration between 0 and 4.8-ág/l, aiming to evaluate a possible toxicological effect. The maximum specific growth rate achieved is 0.221-áh(-1) at 1.6-ág/l of initial diethylketone concentration, suggesting that for higher concentrations an inhibitory effect on the gr ... | 2011 | 21681520 |