| [surgical treatment of urinary bladder cancer]. | ultrasonic transabdominal and transrectal investigations, computed tomography, endoscopic photodynamic studies, polyfocal biopsy of the urinary bladder were used in examination of 1238 patients which were diagnosed to have urinary bladder cancer. 894 patients underwent transurethral resection of the bladder. morphologically, cancer of the urinary bladder has an inductory effect on intact parts of the bladder mucosa. this means that even most radical resection does not eliminate grounds for a new ... | 1999 | 11149341 |
| rna polymerase--promoter recognition. specific features of electrostatic potential of "early" t4 phage dna promoters. | comparative analysis of electrostatic potential distribution for "early" t4 phage promoters was undertaken, along with calculation of topography of electrostatic potential around the native and adp-ribosylated c-terminal domain of rna polymerase alpha-subunit. the data obtained indicate that there is specific difference in the patterns of electrostatic potential distribution in far upstream regions of t4 promoters differing by their response to adp-ribosylation of rna polymerase. a specific chan ... | 2000 | 11149509 |
| stabilization of proteins by enhancement of inter-residue hydrophobic contacts: lessons of t4 lysozyme and barnase. | although the hydrophobic interactions are considered as the main contributors to the protein stability, not much examples of protein stabilization by rational increasing of this type of interactions still can be found in literature. this is partly due to the lack of proper theoretical "measure" of hydrophobic interactions and their changes upon mutations. in the present paper the molecular hydrophobicity potential approach is used to assess how the changes in type and the strength of inter-resid ... | 2000 | 11149522 |
| exploring steric constraints on protein mutations using mage/probe. | when planning a mutation to test some hypothesis, one crucial question is whether the new side chain is compatible with the existing structure; only if it is compatible can the interpretation of mutational results be straightforward. this paper presents a simple way of using the sensitive geometry of all-atom contacts (including hydrogens) to answer that question. the interactive mage/probe system lets the biologist explore conformational space for the mutant side chain, with an interactively up ... | 2000 | 11152136 |
| subclinical hyperthyroidism and the risk of dementia. the rotterdam study. | aims and subjects: we investigated the prospective relationship between thyroid status and the risk of dementia and alzheimer's disease among a random sample of 1843 participants, aged 55 years and over, from the population-based prospective rotterdam study. | 2000 | 11155096 |
| changes of peripheral tissue thyroid hormone metabolism in rats fed with selenium- and vitamin e-deficient artificial semisynthetic diet. | to examine the relationship between selenium (se)- and vitamin e(ve)-deficiency and thyroid hormone (th) metabolic disturbance, especially type i iodothyronine 5'-deiodinase (id-i) activity. | 1998 | 11155682 |
| the efficacy of intraoperative internal intercostal nerve block during video-assisted thoracic surgery on postoperative pain. | video-assisted thoracic surgery (vats) is widely used for many thoracic surgical procedures. post-operative pain is less after vats than after conventional thoracic surgery, but is still significant. the objective of this study was to assess the efficacy of thoracoscopic, internal intercostal nerve block in alleviating immediate postoperative pain. | 2000 | 11156086 |
| dieulafoy's disease of the bronchus in association with a paravertebral neurilemoma. | dieulafoy's disease is a vascular anomaly characterized by the presence of a dysplastic artery that is related to an epithelial ulcer. while it is most frequently a gi condition, occurrence in the bronchus has been reported previously in six cases. we present the case of a 51-year-old man with severe hemoptysis and epistaxis. chest radiographs showed diffuse density in the right base and a mass in the pedicle of t4. the results of bronchoscopy corroborated an active arterial bleeding point in th ... | 2001 | 11157620 |
| brain auditory activation measured by near-infrared spectroscopy (nirs) in neonates. | this study presents a new measure of the hemodynamic changes to an auditory stimulus in newborns. nineteen newborns born at 28-41 wk and aged 1 to 49 d were studied in waking and/or sleeping state, for a median time of 4 min 40 s before, 2 min 40 s during, and 3 min 5 s after an acustic stimulus (tonal sweep of frequency increasing from 2 to 4 khz, intensity 90 db spl) originating 5 cm from the external auditory meatus. the emitter and detector optodes were placed over the left or right temporal ... | 2001 | 11158516 |
| effect of hypothyroidism on pathways for iodothyronine and tryptophan uptake into rat adipocytes. | adipocytes are an important target tissue for thyroid hormone action, but little is known of the mechanisms of thyroid hormone entry into the cells. the present results show a strong interaction between transport of iodothyronines [l-thyroxine (t4), l-triiodothyronine (t3), reverse t3 (rt3)], aromatic amino acids, and the system l amino acid transport inhibitor 2-amino[2,2,1]heptane-2-carboxylic acid (bch) in white adipocytes. system l appears to be a major pathway of iodothyronine and large neu ... | 2001 | 11158928 |
| diverse spatial expression patterns of udp-galnac:polypeptide n-acetylgalactosaminyl-transferase family member mrnas during mouse development. | cell migration and adhesion during embryonic development are complex processes which likely involve interactions among cell-surface carbohydrates. while considerable work has implicated proteoglycans in a wide range of developmental events, only limited attention has been directed towards understanding the 7role(s) played by the related class of mucin-type o-glycans. the initial step of mammalian mucin-type o-glycosylation is catalyzed by a family of udp-galnac:polypeptide n-acetylgalactosaminyl ... | 2000 | 11159923 |
| mutations in the cytoplasmic tail of murine leukemia virus envelope protein suppress fusion inhibition by r peptide. | during viral maturation, the cytoplasmic tail of the murine leukemia virus (mulv) envelope (env) protein undergoes proteolytic cleavage by the viral protease to release the 16-amino-acid r peptide, and this cleavage event activates the env protein's fusion activity. we introduced gly and/or ser residues at different positions upstream of the r peptide in the cytoplasmic tail of the friend mulv env protein and investigated their effects on fusion activity. expression in hela t4 cells of a mutant ... | 2001 | 11160737 |
| multiple four-stranded conformations of human telomere sequence d(ccctaa) in solution. | by detailed nmr analysis of a human telomere repeating unit, d(ccctaa), we have found that three distinct tetramers, each of which consists of four symmetric single-strands, slowly exchange in a slightly acidic solution. our new finding is a novel i-motif topology (t:-form) where t4 is intercalated between c1 and c2 of the other duplex. the other two tetramers have a topology where c1 is intercalated between c2 and c3 of the other parallel duplex, resulting in the non-stacking t4 residues (r-for ... | 2001 | 11160907 |
| cyclin d1 expression in transitional cell carcinoma of the bladder: correlation with p53, waf1, prb and ki67. | normal cell proliferation is closely regulated by proteins called cyclins. one of these, cyclin d1, in combination with its corresponding cyclin-dependent kinase (cdk), is essential for g(1)/s phase transition. cyclin/cdk complexes are generally inhibited by cyclin-dependent kinase inhibitors(ckis), some of which are induced by wild-type p53. the aims of this study were: to investigate levels of cyclin d1 expression in transitional cell carcinoma (tcc) of the bladder; to correlate these results ... | 2001 | 11161387 |
| ictal brain hemodynamics in the epileptic focus caused by a brain tumor using functional magnetic resonance imaging (fmri). | using functional magnetic resonance imaging (fmri) we were able to observe, in detail, ictal brain hemodynamics during epileptic seizure caused by a brain tumor. a 53-year-old man was experencing partial motor seizures of the left side of his face and neck. in a brain mr image a mass lesion was found in the subcortical area of the right frontal lobe. we found focal spikes in his right hemisphere, though dominantly in c4 and t4 regions. fmri investigations were carried out at 1.5 t (ge signa hori ... | 2000 | 11162757 |
| the structure of isometric capsids of bacteriophage t4. | the three-dimensional structure of dna-filled, bacteriophage t4 isometric capsids has been determined by means of cryoelectron microscopy and image reconstruction techniques. the packing geometry of protein subunits on the capsid surface was confirmed to be that of the triangulation class t = 13. the reconstruction clearly shows pentamers, attributed to capsid protein gp24*, surrounded by hexamers of the major capsid protein, gp23*. positions of the accessory proteins, hoc and soc, are also clea ... | 2001 | 11162794 |
| predictive factors in radiotherapy for non-small cell lung cancer: present status. | to evaluate the predictive factors for radiation response in non-small cell lung cancer (nsclc) and the role of such factors in guiding high dose radiation therapy. | 2001 | 11162866 |
| thyroid hormones regulate lipid peroxidation and antioxidant enzyme activities in anabas testudineus (bloch). | the role of thyroid hormones in metabolic pathways are well known. however, their involvement in lipid peroxidation and antioxidant enzyme activities is not known. in this study, the in vivo injection of 6-propylthiouracil (6-ptu) did not alter the concentration of malondialdehyde (mda) and conjugated dienes in liver. the administration of triiodothyronine (t3) or diiodothyronine (t2) increased the peroxidation rate in hypothyroid fish. however, in normal fish, only a high dose of t2 caused incr ... | 2001 | 11163315 |
| structural/functional assignment of unknown bacteriophage t4 proteins by iterative database searches. | among the total of 274 orfs within bacteriophage t4, only half have been reasonably well characterized, and the functions of the rest have remained obscure. in order to predict the molecular functions of the orfs, a position-specific iterated (psi)-blast search of bacteriophage t4 against the sequence database of known 3d structures was carried out. psi-blast is one of the most powerful iterative sequence search methods using multiple sequence alignment, with the ability to detect many more prot ... | 2000 | 11163980 |
| age-related change in peripheral blood t-lymphocyte subpopulations and cytomegalovirus infection in the very old: the swedish longitudinal octo immune study. | results from the previous times (times 1-3) of the swedish longitudinal octo immune study indicated that a combination of high cd8 and low cd4 percentages and poor t-cell proliferation in pbl was associated with a higher 2-year mortality in a sample of very old swedish individuals. the combination of immune parameters was closely related to an inverted cd4/cd8 ratio. in the present study at time 4 (t4) results are reported from the final follow-up of this longitudinal study, 8 years after it was ... | 2000 | 11164473 |
| electroencephalographic comparison of veterans with combat-related post-traumatic stress disorder and healthy subjects. | various investigations prove the biological basis for post-traumatic stress disorder (ptsd). there are only a few quantitative electroencephalographic (qeeg) studies of patients with ptsd. the aim of this investigation is to find out whether there are differences in qeeg parameters between ptsd patients and healthy subjects. the experimental group was comprised of 18 veterans with ptsd, and 20 healthy non-veterans were included in the control group. after informed consent was obtained, they were ... | 2001 | 11165355 |
| analyses of spontaneous mutations of cloned gene 49 of phage t4. | holliday structure resolving enzyme endonuclease vii (endo vii) of phage t4 is highly toxic for e. coli when expressed outside of the phage infection environment. as a consequence, plasmids with a mutated gene 49, the gene which encodes for endo vii, can be easily isolated and characterised. we have isolated and characterised 400 survivors from independent transformations with a plasmid carrying gene 49 under the control of the t7 promoter. the majority had mutated gene 49 by is10 insertions whi ... | 2001 | 11166037 |
| median frequency of the electromyographic signal: effect of time-window location on brief step contractions. | the purpose of this study was to determine, for different back muscles, if the median frequency (mf) of the electromyographic (emg) power spectrum changes according to the position of the time window during a 5 s step contraction. twenty males with no known back problems were standing upright in a dynamometer allowing lower limb and pelvis stabilization. trunk extension efforts were performed by pushing on a force platform positioned at the t4 level while the extension moment at l5/s1 was displa ... | 2001 | 11166609 |
| isocaloric carbohydrate deprivation induces protein catabolism despite a low t3-syndrome in healthy men. | dietary carbohydrate content is a major factor determining endocrine and metabolic regulation. the aim of this study was to evaluate the relation between thyroid hormone levels and metabolic parameters during eucaloric carbohydrate deprivation. we measured thyroid hormone levels, resting energy expenditure (by indirect calorimetry) and urinary nitrogen excretion in six healthy males after 11 days of three isocaloric diets containing 15% of energy equivalents as protein and 85%, 44% and 2% as car ... | 2001 | 11167929 |
| microscopic hematuria as a screening marker for urinary tract malignancies. | although a mass screening urinalysis is a widely accepted procedure, it has not yet been shown if microhematuria is an appropriate and useful screening marker for urologic malignancies. | 2001 | 11168689 |
| electrophysiology and intelligence: the electrophysiology of intellectual functions in intellectual disability. | the electroencephalograms (eegs) of 127 subjects with intellectual disability were analysed with regarding to their correlation to intellectual functions in order to further understand the relationships between eeg and intelligence. the eeg frequency spectrum was subdivided into 15, 2-hz-wide bands and was recorded from electrodes f1, fpz, fp2, f7, f3, fz, f4, f8, t3, c3, cz, c4, t4, t5, p3, pz, p4, t6, o1, oz and o2. patterns of acorrelation showed several similarities when compared to other an ... | 2001 | 11168778 |
| predictors of response and survival after concurrent chemotherapy and radiation for locally advanced squamous cell carcinomas of the head and neck. | the objective of this study was to determine prognostic factors for response and survival on three consecutive institutional trials utilizing concurrent chemotherapy and radiation for locally advanced squamous cell carcinomas of the head and neck (scchn). | 2001 | 11169937 |
| comparative genomic hybridization analysis of genetic aberrations associated with development and progression of biliary tract carcinomas. | little is known about genetic aberrations associated with development and progression of biliary tract carcinomas. | 2001 | 11169940 |
| novel rapid method for visualization of extent and location of aerosol contamination during high-speed sorting of potentially biohazardous samples. | containment of potentially biohazardous aerosols that result from high-speed sorting of human cells has been an increasingly important problem in analytical cytometry. the current method for assessing the efficiency of aerosol containment involves detection of aerosols containing sorted t4 bacteriophage on lawns of t4-susceptible escherichia coli on plates that are placed in and around the sort area. although this method is sensitive, it is time consuming and involves maintenance and handling of ... | 2001 | 11170110 |
| [videocardioscopy: a new way to assess resectability of ct4 lung cancer by vascular invasion]. | to demonstrate the usefulness of intrapericardial inspection of pulmonary vessels by video assisted thoracoscopy (vat), for determining whether suspicion indicated by computed axial tomography (cat) or magnetic resonance (mr) scanning is justified or not. | 2000 | 11171433 |
| association between serum concentrations of hexachlorobenzene and polychlorobiphenyls with thyroid hormone and liver enzymes in a sample of the general population. | hexachlorobenzene (hcb) is a highly lipophilic organochlorine compound of widespread environmental occurrence, that accumulates in the biological system. it affects the porphyrine metabolism, thyroid hormones, and the liver function in animals. although hcb is one of the most common organochlorine compound in humans, little investigation on its health effects has been done. polychlorobiphenyls (pcbs) are also widespread toxic environmental contaminants. the aim of the present study was to invest ... | 2001 | 11171930 |
| combined spinal and epidural anesthesia with low doses of intrathecal bupivacaine in women with severe preeclampsia: a preliminary report. | the purpose of our study was to evaluate the quality of anesthesia for cesarean delivery (cd), analgesia for labor (la), hemodynamic changes, and neonatal effects of combined spinal and epidural anesthesia (cse) with low intrathecal doses of bupivacaine and fentanyl in patients with severe preeclampsia. | 2001 | 11172511 |
| bacteriophage t4 proteins replicate plasmids with a preformed r loop at the t4 ori(uvsy) replication origin in vitro. | bacteriophage t4 dna replication proteins catalyze complete unidirectional replication of plasmids containing the t4 ori(uvsy) replication origin in vitro, beginning with a preformed r loop at the position of the origin r loop previously identified in vivo. t4 dna polymerase, clamp, clamp loader, and 32 protein are needed for initial elongation of the rna, which serves as the leading-strand primer. normal replication is dependent on t4 41 helicase and 61 primase and is strongly stimulated by the ... | 2001 | 11172709 |
| neoadjuvant estramustine and etoposide followed by concurrent estramustine and definitive radiotherapy for locally advanced prostate cancer: feasibility and preliminary results. | current therapy for locally advanced prostate cancer is suboptimal. a treatment regimen was designed to improve systemic control by neoadjuvant targeting of hormone-sensitive and -insensitive micrometastatic disease and to improve local control by escalating the biologic effective dose to the prostate using estramustine (emp) concurrently with radiotherapy. | 2001 | 11172951 |
| transformation of a fragment of beta-structural bacteriophage t4 adhesin to stable alpha-helical trimer. | gene product 12 of bacteriophage t4, adhesin, serves to adhere the virus to host cells. adhesin is a fibrous homotrimer, and a novel tertiary structure element, a beta-helix, is supposed to be a major structural feature of this protein. we have constructed two truncated gp12 mutants, 12n1 and 12n2, containing 221 and 135 n-terminal residues, respectively. when expressed in e. coli cells, these gp12 fragments formed labile beta-structural trimers. another hybrid protein, 12fn, containing 179 n-te ... | 2000 | 11173503 |
| depressive symptoms in hypothyroid disorder with some observations on biochemical correlates. | lumbar punctures and ratings of depressive symptoms were done in hypothyroid patients before and during l-thyroxine therapy. before treatment, the most prominent symptoms were concentration difficulties, lassitude, and reduced sexual interest. all patients suffered from sleep disturbances. suicidal thoughts did not occur at all. inner tension was negatively correlated with the anxiogenic cholecystokinin tetrapeptide (cck-4) in the cerebrospinal fluid (csf), while reduced sexual interest was nega ... | 2001 | 11174048 |
| radioiodine treatment of metastatic differentiated thyroid cancer in patients on l-thyroxine, using recombinant human tsh. | this study tested the hypothesis that administration of human recombinant thyroid-stimulating hormone (rhtsh: thyrogen, thyrotropin alpha) could promote iodine-131 ((131)i) uptake in the therapy for metastatic or locally invasive differentiated thyroid cancer (dtc), obviating l-thyroxine suppressive therapy (l-t4) withdrawal and hypothyroidism in patients with advanced disease. | 2001 | 11174831 |
| expression of endothelin receptor a associated with prostate cancer progression. | we determined the role of endothelin receptors in prostate cancer progression. | 2001 | 11176535 |
| multivariate analysis of risk factors for neck metastases in surgically treated parotid carcinomas. | to analyze risk factors for neck metastases in patients with parotid carcinomas. | 2001 | 11177015 |
| selenium influences growth via thyroid hormone status in broiler chickens. | as there is a possibility that se influences the growth of animals via thyroid hormone metabolism, the following three experiments were undertaken in order to determine the effects of dietary se on growth, skeletal muscle protein turnover and thyroid hormone status in broiler chickens. broiler chickens were raised on a se-deficient diet until 12 d of age and then used for the experiments. in experiment 1, twenty-eight birds were randomly assigned to four groups and fed purified diets with the fo ... | 2000 | 11177187 |
| rectal cancer: ct local staging with histopathologic correlation. | many surgical options, eventually combined with neoadjuvant therapy, are available for the treatment of rectal cancer. preoperative staging is essential to plan the correct treatment. our aim was to evaluate the diagnostic accuracy of computed tomography (ct) in the local staging of rectal cancer. | 2001 | 11178688 |
| development and characterization of a new polyclonal antibody specifically against tissue inhibitor of metalloproteinases 4 in human breast cancer. | tissue inhibitors of metalloproteinases (timps) may regulate extracellular matrix turnover and cellular functions by modulating matrix metalloproteinase (mmp) activity and cell proliferation and apoptosis. to investigate the locations and functions of timp-4 in human breast cancer, a highly specific polyclonal anti-timp-4 peptide antibody (pab-t4-s61) was developed. the potency and specificity of the purified igg were characterized by an enzyme-linked immunosorbent assay, immunoblot, and immunoh ... | 2001 | 11178975 |
| nad+ inhibits the self-splicing of the group i intron. | we investigated the effects of the coenzyme nad+ (nicotinamide adenine dinucleotide) and its analogs on the self-splicing of primary transcripts of the phage t4 thymidylate synthase gene (td). of all the nicotinamide coenzymes and analogs tested, nadp+ was the strongest inhibitor, with a potency approximately threefold that of nad+. kinetic analysis demonstrated that nad+ acts as a mixed type noncompetitive inhibitor for the td intron rna with a k(i) of 4.1 mm. the splicing specificity inhibitio ... | 2001 | 11178981 |
| effects of low mercury vapour exposure on the thyroid function in chloralkali workers. | forty-seven chloralkali workers exposed to mercury vapour for an average of 13.3 years were compared with 47 referents matched for age in a cross-sectional study of thyroid function. the mean urinary mercury concentration in the exposed workers was low compared with other studies of chloralkali workers: 5.9 nmol mmol-1 creatinine (range 1.1-16.8) vs 1.3 nmol mmol-1 creatinine (range 0.2-5.0) in the reference group. the median serum concentration of reverse triiodothyronine (rt3) was statisticall ... | 2000 | 11180271 |
| in vitro selection of kinase and ligase deoxyribozymes. | exploration of the limits of biocatalysis has led to the discovery that dna has significant potential for enzymatic function. this makes possible the construction of dna enzymes or "deoxyribozymes" for catalyzing various chemical reactions that could be used to address fundamental questions in biocatalysis or that could find unique applications in biotechnology. of significant interest are self-modification reactions, given the fundamental role that dna serves in modern living systems. recently, ... | 2001 | 11181037 |
| multicenter phase i-ii trial of docetaxel, cisplatin, and fluorouracil induction chemotherapy for patients with locally advanced squamous cell cancer of the head and neck. | we conducted a phase i-ii, multi-institutional trial to determine the maximum-tolerated dose (mtd) of cisplatin in an induction chemotherapy regimen of docetaxel, cisplatin, and fluorouracil for squamous cell cancer of the head and neck (scchn) and to determine the safety, tolerability, and efficacy of the regimen at mtd. | 2001 | 11181674 |
| postoperative radiotherapy for squamous cell carcinoma of the maxillary sinus: analysis of local control and late complications. | this retrospective study was conducted to analyze the local control and late complications in patients with squamous cell carcinoma of the maxillary sinus treated with postoperative radiation therapy following surgery. between 1979 and 1998, 41 patients with squamous cell carcinoma of the maxillary sinus were treated with postoperative irradiation following partial or total maxillectomy. tumor classification according to the tnm classification of the international union against cancer (1997) was ... | 2001 | 11182047 |
| effect of milking frequency on oxytocin release and milk production in dairy ewes. | the experiment was conducted to investigate the effectiveness of milking stimulus on oxytocin release and to compare the effect of milking frequency on plasma levels of oxytocin and milk parameters. twelve lacaune ewes were subjected to six treatments (t1, t2, t3, t4, t5 and t7 daily milkings) during 6 days. at each milking, blood was sampled and plasma oxytocin levels were determined by enzyme immunoassay. baseline levels of oxytocin were similar for all milking frequencies. the start of milkin ... | 2001 | 11182311 |
| thyroid function and obesity in children and adolescents. | the aim of this retrospective study was to investigate the frequency of thyroid dysfunction as assessed by tsh, t3 and t4 in a large cohort of 290 obese and 280 healthy children. in addition, thyroid autoantibodies were measured in random subgroups of 123 obese and 80 control children, iodine excretion in 50 and thyroid volume in 23 of the obese children. elevated tsh levels (>4 u/l) were found in 22 obese children (7.5%), but only in one control (0.3%). the medians of tsh and t3 concentrations ... | 2000 | 11182630 |
| differences between the effects of thyroxine and tetraiodothyroacetic acid on tsh suppression and cardiac hypertrophy. | we earlier reported marked qualitative differences between the effect of 3,5,3'-tri-iodothyroacetic acid (triac) and tri-iodothyronine (t3) on cardiac hypertrophy at equivalent thyroid-stimulating hormone (tsh)-suppressive doses. we have now extended these studies to specific cardiac parameters. due to its rapid metabolic clearance rate, triac is not suitable for tsh suppression and therefore the slowly metabolized 3,5,3',5'-tetraiodothyroacetic acid (tetrac), the precursor of triac, was studied ... | 2001 | 11182751 |
| human slow cortical potential in association with voluntary jaw movements. | the distribution of a readiness potential (rp), i.e., a negative-going slow cortical potential (scp) preceding the onset of voluntary movements, was studied in association with self-paced voluntary jaw-closing and jaw-opening movements in 4 healthy human subjects. a negative-going scp starting at ca.1.5-2.0 s preceding the onset of the self-paced jaw-opening and jaw-closing movements was found at all the recorded sites of fz, cz, pz, f3, f4, f7, f8, c3, c4, t3 and t4. the rp was maximum in ampli ... | 1998 | 11186211 |
| efficient inhibition of escherichia coli rna polymerase by the bacteriophage t4 asia protein requires that asia binds first to free sigma70. | the bacteriophage t4 asia protein inhibits transcription from host and phage early promoters and is required, along with the t4 mota protein, for activation of phage middle promoters. during infection, asia is found in a tight association with the sigma70 subunit of rna polymerase. we show that asia binds rapidly to free sigma70 at either 4 degrees c or 30 degrees c to form an asia-sigma70 complex that with core efficiently reconstitutes the asia-inhibited rna polymerase. in contrast, asia does ... | 2000 | 11188759 |
| [indications and results of radical treatment of gallbladder carcinoma]. | extended cholecystectomy is the only chance of a cure for patients with locally advanced cancer of the gallbladder. the aim of the study was to evaluate the short- and long-term results of surgical treatment and to define the prognostic factors associated with better survival. we conducted a retrospective study in 81 patients with gallbladder cancer admitted to our surgical department from 1985 to 1999. radical surgery was performed on 39 patients. the type of surgical treatment was based on the ... | 2000 | 11190541 |
| chronic stress influences the immune system through the thyroid axis. | the aim of the present work was to analyze the effect of chronic stress on thyroid axis and its influence on the immune response. for this purpose a murine model of chronic stress was developed to evaluate and to correlate thyroid hormone levels with humoral alloimmune response. results show a reduction in serum levels of thyroid hormones, specially a significant decrease in serum levels of triiodotyronine (t3) in stressed animals. on the other hand, alloimmunization was not able to induce an ea ... | 2000 | 11191624 |
| [supplement with target hormone in aged patients with endocrine dysfunction: thyroid hormone replacement therapy]. | hypothyroidism is not rare in aged people. hashimoto thyroiditis and radiation to the neck region are main causes of hypothyroidism in aged subjects. the symptoms are slowly progressing, and are similar to those of the aged subjects free from thyroid disease. thus, it is difficult to make a diagnosis of hypo-thyroidism in the elderly. administration of t4 (not t3) to patients with hypothyroidism completely releases them from symptoms, indicating that supplement with t4 is important in maintainin ... | 2000 | 11193357 |
| primary carcinoma of the subglottic larynx. | primary cancer of the subglottic region is very rare and delay in diagnosis often leads to a poor prognosis. we retrospectively reviewed 49 patients with primary cancer of the subglottic larynx observed from 1969 to 1993 in the orl clinic of the university of florence. this number constitutes 1.6% of all laryngeal cancers observed during this period. four (8.2%) patients were stage t1, 13 (26.5%) t2, 27 (55.1%) t3, and 5 (10.2%) t4. forty-one patients were eligible for assessing the disease-free ... | 2000 | 11195034 |
| acquired lumbar kyphosis caused in adults by primary paraspinal myopathy. epidemiology, computed tomography findings, and outcomes in a cohort of 23 patients. | acquired lumbar kyphosis in adults, also called bent spine syndrome (bss), is probably due to primary tardive myopathy affecting the paraspinal muscles. | 2000 | 11195316 |
| [oligomerization of dna-(adenine-n6)-methyltransferase from phage t4 and its effect on the catalytic characteristics of the enzyme]. | the structural and catalytic properties of the phage t4 dna-(adenine-n6)-methyltransferase (ec 2.1.1.72) were studied at different enzyme-substrate concentration ratios by chemical cross-linking of the protein subunits and by measuring the presteady state kinetics of the reactions. various structural states of the methyltransferase were correlated with its catalytic activity, and it was shown that the oligomeric forms of the enzyme are catalytically active but are characterized by the reaction p ... | 2000 | 11195593 |
| the incidence and clinical characteristics of symptomatic propylthiouracil-induced hepatic injury in patients with hyperthyroidism: a single-center retrospective study. | although symptomatic propylthiouracil (ptu)-induced hepatic injury is known to be rare, there have been few reports about its exact incidence in patients with hyperthyroidism. we tried to evaluate its incidence in a single center and its clinical course. | 2001 | 11197248 |
| synthesis and characterization of a tetranucleotide analogue containing alternating phosphonate-amide backbone linkages. | described herein is the synthesis and characterization of a tetranucleotide, 5'-dc-phosphonate-t-amide-t-ophosphonate-dc (iii), in which the c-t and t-c steps contain a phosphonate backbone bond and t-t is a peptide nucleic acid dimer unit (neutral backbone). the 5'- and 3'-oh groups of the tetramer can be further derivatized and, thus, the compound is a potential building block for longer oligonucleotides which will contain alternating backbone modifications at designated positions. the synthes ... | 2001 | 11197331 |
| physiological responses of pre-ruminant kid goats and lambs to different environmental temperatures. | the purpose of this study was to establish the metabolic behaviour of both preruminant kid goats and lambs, when they are kept at different environmental temperatures (12, 24 and 30 degrees c). the animals were fed ad libitum with a milk replacer for the first two months of life. blood samples were taken from all the animals on days 30, 40, 50 and 60 post partum, to determine serum levels of glucose, insulin, free fatty acids, triiodothyronine (t3) and thyroxine (t4), both when fasting and 4 hou ... | 2000 | 11198159 |
| [radiation therapy after mastectomy--interdisciplinary consensus puts and end to a controversy. german society of senology]. | recent publications of the danish breast cancer cooperative group together with data from the british columbia trial have stirred major discussions concerning the role of radiation therapy after mastectomy. different treatment approaches are to be found even within the same cancer center. the german society of senology, a cooperative group of all medical disciplines involved in the treatment of breast cancer, has therefore worked out a consensus statement. | 2001 | 11200107 |
| sympathetic overactivity in the development of eyelid retraction in a patient with euthyroid graves' disease evaluated by accommodation. | it is known that measurement of accommodation is useful to evaluate the sympathetic activity of intraocular muscles. to find if sympathetic overactivity is involved in eyelid retraction in euthyroid graves' disease, we measured accommodation in two patients with this disease, whose serum concentrations of free t3, free t4 and tsh were within reference ranges. accommodation was measured with a computer-assisted infrared optometer with an iriscoder, and the results were expressed as the change in ... | 2000 | 11200944 |
| basedow's disease and chronic ulcerative colitis: a case report and review of the japanese literature. | a case of basedow's disease, that developed after successful treatment of ulcerative colitis with a total colectomy, is presented, along with a review of the japanese literature on the coexistence of hyperthyroidism and ulcerative colitis. a 26-year-old man was referred to our department, complaining of general fatigue, appetite loss, and palpitation. at age 14, blood was discovered in his stool and a diagnosis of ulcerative colitis was made. since then, he has been treated with salazosulfapyrid ... | 2001 | 11201369 |
| carnitine is a naturally occurring inhibitor of thyroid hormone nuclear uptake. | carnitine (3-hydroxy-4n-trimethylammoniumbutanoate) is a naturally occurring quaternary amine that is ubiquitous in mammalian tissues (concentrations in the order of mm). based on limited studies of approximately 40 years ago, carnitine was considered to be a peripheral antagonist of thyroid hormone (th) action. these interesting observations have not been explored. to study the biologic basis of this effect, we tested the following possibilities in three th-responsive cell lines: (1) inhibition ... | 2000 | 11201848 |
| is excessive weight gain after ablative treatment of hyperthyroidism due to inadequate thyroid hormone therapy? | there is controversy about the correct dose and form of thyroid hormone therapy for patients with hypothyroidism. despite restoration of serum thyrotropin (tsh) concentrations to normal, many patients complain of excessive weight gain. we have compared weight at diagnosis of hyperthyroidism with that when euthyroid, evidenced by a stable, normal serum tsh concentration, with or without thyroxine (t4) replacement therapy, in patients treated with an 18-month course of antithyroid drugs (43 patien ... | 2000 | 11201857 |
| a pilot study of postoperative chart and chartwel in head and neck cancer. | a phase ii pilot study of continuous hyperfractionated accelerated radiotherapy (chart)/chart weekend less (chartwel) was carried out in the postoperative treatment of patients with squamous cell carcinoma of the head and neck. twenty-four patients (17 male, seven female) with a median age of 64 years (range 34-80) were treated with postoperative radiotherapy between 1991 and 1999. all patients presented with primary squamous cell carcinoma, which, at surgery, had shown adverse pathological fact ... | 2000 | 11202092 |
| bound transcription factor suppresses photoproduct formation in the nf-kappa b promoter. | the relationship between purified transcription factor p50 binding and ultraviolet light-induced dna damage formation in the nf-kappa b promoter element was investigated. the effect of bound transcription factor on cyclobutane dimer formation was quantified using maxam-gilbert analysis of irradiated substrate digested with t4 phage endonuclease v. two methods were employed for cleaving (6-4) photoproducts. sites of (6-4) photoproducts cleaved by piperidine showed a general suppression in the pre ... | 2001 | 11202359 |
| changes in pressure-pain thresholds of the jaw muscles during a natural stressful condition in a group of symptom-free subjects. | to investigate the effects of a natural emotional stressor on pressure-pain thresholds (ppts) of the masticatory muscles of symptom-free subjects. | 2000 | 11203761 |
| frequency of oral manifestations in children infected with human immunodeficiency virus. | the main objective of this study was to investigate the frequency of oral manifestations in children with acquired immunodeficiency syndrome. it also attempted to correlate the presence of lesions with the patient's degree of immunosuppression, as measured by the t4/t8 ratio. | 2000 | 11203960 |
| hyperlipidemia and type i 5'-monodeiodinase activity: regulation by selenium supplementation in rabbits. | male new zealand white rabbits were divided into three groups: (i) control, (ii) high-fat-diet (hfd) fed, and (iii) hfd fed + selenium supplemented. after 3 mo of treatment, there was a significant increase in serum cholesterol and triglycerides in the hfd-fed group as compared to the control. however in the selenium (se)-supplemented group, the levels of serum cholesterol and triglycerides were significantly less as compared to group ii. hfd feeding resulted in decreased serum se levels, but su ... | 2000 | 11204465 |
| assessment of thyroid function in pesticide formulators. | thirty male pesticide formulators exposed to the dust and liquid formulation of endosulfan, quinalphos, chlorpyriphos, monocrotophos, lindane, parathion, phorate, and fenvalerate and 20 comparable control subjects from the same area of study were examined for the evaluation of thyroid function tests. the level of tsh was elevated (about 28%) in pesticide formulators as compared to a control group, but the increase was statistically insignificant. based on the individual tsh measurement, 3 of 30 ... | 2000 | 11204551 |
| rectal cancer: integrating oxaliplatin into chemoradiation studies. | the current standard of care for patients with stage t3 rectal cancer is adjuvant combined-modality treatment with radiation and fluorouracil (5-fu)-based chemotherapy. although data from randomized phase iii trials comparing preoperative and postoperative combined-modality therapy are lacking, preoperative therapy is an option in t3 disease and can be considered the standard of care for t4 disease. given its effects in reducing systemic disease in stage iv rectal cancer and its potential for ra ... | 2000 | 11204662 |
| endocrine relationships of meishan and white composite females after weaning and during the luteal phase of the estrous cycle. | endocrine differences between european crossbred and chinese meishan females have been somewhat modest. indwelling jugular cannulas were placed in meishan (n = 7) and white composite (n = 6) multiparous sows before weaning, and blood was sampled from 4 h before to 240 h after removal of litters. temporal changes in fsh, triiodothyronine (t3), and tetraiodothyronine (t4) after weaning differed between meishan and white composite sows. plasma cortisol concentrations were higher in meishan sows tha ... | 2001 | 11204698 |
| clinicopathological study of mucinous gastric carcinoma. | mucinous gastric carcinoma (muc) is relatively a rare subtype of gastric carcinoma, and there has been considerable controversiy over the prognosis of this type of carcinoma. in this study we tried to analyze the clinicopathological differences between muc and other types of gastric carcinoma (non-muc) and determine whether muc is associated with a worse prognosis than non-muc. | 2000 | 11204957 |
| epidoxorubicin and paclitaxel as primary chemotherapy for t > 3 cm and t4 breast cancer patients. | the anthracyclines/taxanes combination is often used in adjuvant and advanced breast cancer. | 2000 | 11205227 |
| altered response to thyroid hormones by breast and ovarian cancer cells. | in this study, l-thyroxine (t4), 3',3,5-triiodo-l-thyronine (t3), 3,5-diiodo-l-thyronine (t2), reverse t3; 3',5',3-triiodo-l-thyronine (rt3) and transferrin were added to breast cancer cell lines hs 578t, mda-mb-231, mda-mb-468, and t-47d and ovarian cancer cell line ovcar-3 to test the response to cell proliferation. | 2000 | 11205239 |
| [multiband spectral analysis and standardized markers of the slope of pediatric electroencephalograms]. | the infantile electroencephalogram has been studied in connection with many cognitive disabilities. although normal resting infantile bioelectric recordings are known, the cortical distribution of electroencephalographic frequencies during other sensory and/or cognitive mental processes is less well known. | 2000 | 11205544 |
| clinical and immunological spectrum of partial digeorge syndrome. | we present four cases of digeorge syndrome diagnosed at our center. onset occurred during the neonatal period and was associated with severe congenital heart disease. in case 1, the patient had heart disease and absence of thymus. total t-lymphocytes were 34%; total t4-lymphocytes were 27%. stimulation test with phytohemagglutinin (pha), concanavalin a (cona) and pokeweed mitogen were negative. microdeletion was found in the chromosome 22q11 region. the second case involved heart disease, micros ... | 2000 | 11206936 |
| adaptation of tobacco budworm heliothis virescens to proteinase inhibitors may be mediated by the synthesis of new proteinases. | the tobacco budworm heliothis virescens is adapted to feed on tobacco leaves that have proteinase protein inhibitors (pis). to study this adaptation, the midgut proteinases of heliothis virescens larvae reared on artificial pi-free diet and on tobacco leaves were compared using ion exchange chromatography, hydrophobic chromatography, gel filtration and polyacrylamide gel electrophoresis at different conditions. sds polyacrylamide-gradient gel electrophoresis (sds-page) and kinetic studies shown ... | 2001 | 11207448 |
| levels of serum total cholesterol and ldl-cholesterol in patients with major depression in acute period and remission. | the aim of the study was to determine the level of total cholesterol and ldl-cholesterol in blood samples taken from 102 patients with recurrent major depression (according to dsm-iv). the analysis was performed during the acute period of major depression in 3 subgroups: with and without suicidal ideation (s+, s-), and after suicidal attempts (as), and during remission of depressive symptoms. putative correlations between the level of total cholesterol and severity of depressive symptoms and bet ... | 2000 | 11208367 |
| phase i trial of concurrent tirapazamine, cisplatin, and radiotherapy in patients with advanced head and neck cancer. | to determine the maximum-tolerated dose of tirapazamine when combined with cisplatin and radiation in patients with t3/4 and/or n2/3 squamous cell carcinoma of the head and neck. | 2001 | 11208848 |
| cognitive and motor functions of iodine-deficient but euthyroid children in bangladesh do not benefit from iodized poppy seed oil (lipiodol). | iodine supplementation before pregnancy in iodine-deficient women prevents cretinism and neuromotor deficits in their offspring. it is unclear whether iodine supplementation benefits cognitive function in iodine-deficient school-aged children. we therefore conducted a double-blind, randomized, controlled trial of the effects of iodized poppy seed oil (lipiodol) on cognitive and motor function and weight gain of iodine-deficient school children. the study was conducted with 305 children in grades ... | 2001 | 11208941 |
| renin-angiotensin system contribution to cardiac hypertrophy in experimental hyperthyroidism: an echocardiographic study. | the objective of this study was to evaluate, using echocardiography, the involvement of the renin-angiotensin system (ras) in left ventricular (lv) hypertrophy development in experimental hyperthyroidism. thyrotoxicosis was produced by a daily intraperitoneal injection of l-thyroxine (t4), 0.1 mg/kg per day for 15 days in wistar rats. control (euthyroid) rats received intraperitoneal daily injection of the thyroxine solvent. two series of experiments were performed. in the first series, euthyroi ... | 2001 | 11209999 |
| preoperative concurrent chemoradiotherapy against muscle-invasive bladder cancer: results of partial cystectomy in elderly or high-risk patients. | good local control has been reported in cases of muscle-invasive bladder cancer treated by chemoradiotherapy and transurethral resection (tur). however, definitive irradiation or extensive chemotherapy is often intolerable for elderly or poor-risk patients. we report here benefits of partial cystectomy after concurrent low-dose chemoradiotherapy for high-risk patients. | 2000 | 11210165 |
| oral squamous cell carcinoma: a retrospective study of 740 cases in a brazilian population. | the purpose of this study was to evaluate, retrospectively, the profile of patients with oral squamous cell carcinoma. they were referred to two hospitals of belo horizonte (mg, brazil) between 1986 and 1996. the medical records of 740 patients presenting oral squamous cell carcinoma were reviewed in detail. the tnm classification and staging, age, gender, race, occupation, and site of the primary tumor were recorded. the male-to-female ratio was 4.8:1. the mean age was 58.6 years. most of the p ... | 2001 | 11210254 |
| [coronary insufficiency and metabolic hormone ratio in patients with myocardial infarction in the course of a long prospective study]. | an angiographic study of coronary bed status and relationships of insulin, cortisol, somatotropin (st), t3, t4, glucose in 109 q-wave myocardial infarction (mi) patients aged 47 +/- 0.8 years was performed at rest and during veloergometric stress-test ischemia modelling of exercise tolerance in early (21-26 days) and late (> 5 years) follow-up. the hormones level was found to be significantly related to coronary insufficiency as a result of coronary atherosclerosis both at rest and during muscul ... | 2000 | 11210345 |
| nicorandil accelerates recovery of neuromuscular block caused by vecuronium. | to examine the effect of nicorandil, a k atp channel agonist, on neuromuscular block caused by vecuronium in patients anesthetized with nitrous oxide, oxygen, isoflurane, and fentanyl. | 2001 | 11212045 |
| effect of deboning time, muscle tensioning, and calcium chloride marination on texture characteristics of chicken breast meat. | tenderness is the most important organoleptic characteristic of meat, and various methods have been developed to improve it. the purpose of this experiment was to evaluate the effect of different conditioning treatments of broiler carcasses on ph, cooking losses, shear values, r-values, and sensory tenderness of breast meat. all measurements were collected for breast muscle as follows: after 24 h of carcass aging (t1); after 24 h of carcass aging with muscle tensioning (t2); after 24 h of carcas ... | 2001 | 11214330 |
| role of thyroidal and testicular hormones in regulation of tissue respiration in male air-breathing fish, clarias batrachus (linn.). | in vivo and in vitro effects of thyroidal hormones (mit, dit, t3, t4), propyl thiouracil (ptu), testosterone and cyproterone acetate were studied on the rate of tissue (liver, muscle, kidney and brain) respiration of adult male c. batrachus during winter and summer/rainy seasons. monoiodotyrosine (mit) and diiodothyrosine (dit) increased the respiratory rate in a dose-dependent and temperature-independent manner. triiodothyronine (t3) and thyroxine (t4) stimulated tissue respiration during summe ... | 2000 | 11215315 |
| the deiodinase family: selenoenzymes regulating thyroid hormone availability and action. | thyroid hormones control growth, development, differentiation and metabolism in vertebrates. most of the actions of the active thyroid hormone t3 (3,5,3'-triiodo-l-thyronine) are exerted via ligand-activated nuclear t3 receptors. activation of the secretory product of the thyroid gland, l-thyroxine (3,3',5,5'-tetraiodo-l-thyronine), or t4, is catalyzed by two enzymes, iodothyronine-5'-deiodinases type i and type ii. inactivation of t4 and t3 occurs via type iii iodothyronine-5-deiodinase and to ... | 2000 | 11215512 |
| effects of microsomal enzyme inducers on thyroid follicular cell proliferation and thyroid hormone metabolism. | the effects of microsomal enzyme inducers on thyroid hormone homeostasis and the thyroid gland are of concern. we have investigated the effects of microsomal enzyme inducers on thyroid follicular cell proliferation and thyroid hormone metabolism in rats. we have shown that small increases in serum tsh can result in large increases in thyroid follicular cell proliferation. furthermore, only those microsomal enzyme inducers that increase serum tsh--that is, phenobarbital (pb) and pregnenolone-16al ... | 2001 | 11215682 |
| effects of isoflavone supplement on healthy women. | effects of the isoflavone supplement on hormonal states in young premenopausal women were studied by cross-over study design. administration of 20 mg or 40 mg isoflavones (if) by tablets, of which 1 g contained 43.5 mg daidzein, 6.0 mg genistein, 24.0 mg glycitein, to 40 young female students for one month caused a prolonged menstruation in 60% of young women, shortened menstruation in 20% of young women, 17% remained unchanged and 3% became irregular. larger dose tended to elongate more, but 17 ... | 2000 | 11216491 |
| effects of exposure of lactating female rats to polychlorinated biphenyls (pcbs) on testis weight, sperm production and sertoli cell numbers in the adult male offspring. | to investigate the effects of intermittent and continuous exposure of lactating rats to aroclor 1242 (a pcb congener), testis weight, daily sperm production (dsp) and sertoli cell number per testis were examined in the adult male offspring. thyroxine (t4) was also measured because of the well-documented effects of polychlorinated biphenyls (pcbs) on this hormone. in experiment 1.3 groups of lactating female rats received daily subcutaneous injections of low (0.8 mg) or high (1.6 mg) doses of aro ... | 2001 | 11217063 |
| [postoperative complications after radiochemotherapy or chemotherapy for bronchial cancers]. | the purpose of this study was to evaluate the risk of lung cancer surgery, following induction chemo and/or radiotherapy. | 2000 | 11217503 |
| [prostatic biopsies: technical modalities and conditions of the test]. | histological examination of prostate biopsies is necessary for the diagnosis of prostate cancer. the technical modalities and examination conditions of prostate biopsies were evaluated in the light of a review of the literature and the conclusions are presented in the form of standards, options and recommendations. standards: the standard or modified protocol of 6 sextant biopsies, applied as first-line investigation in all cases of stage t1c or t2 prostate cancer is a standard procedure and rem ... | 2000 | 11217574 |
| thyroid function in the intensive care unit setting. | in sum, there is no convincing evidence yet published supporting the utility of t4 or t3 administration in patients with nonthyroidal illness. the authors recognize that evidence accrued in one disease state may not be applicable to others and that, although these studies are difficult to perform, further large scale prospective studies need to be performed. the issue of t3 treatment will not be resolved satisfactorily until more definitive data are available. until that time, there may be rare ... | 2001 | 11219234 |
| [thyroid status and clinical features of osteoarthrosis in the region of goiter endemia]. | the study of osteoarthrosis (oa) course in the region of goiter endemia regarding thyroid status. | 2000 | 11220868 |
| [effect of s-adenosyl-l-methionine and its analogs on site-specific binding dna-(adenine-n6)-methyltransferase from t4 phage to the oligonucleotide substrate]. | using fluorescence of 2-aminopurine-substituted oligonucleotide duplexes, "flipping" of the target base in the process of interaction of t4 dna-(adenine-n6)-methyltransferase (ec 2.1.1.72) with the substrate double-stranded dna was revealed. it was shown that s-adenosyl-l-methionine, the methyl group donor, induces the reorientation of the enzyme relative to the asymmetrically modified recognition site. | 2000 | 11221270 |
| the evaluation of thyroid and islet autoantibodies in type 1 diabetes mellitus. | type 1 diabetes mellitus is an autoimmune disease in which the presence of different autoantigens can often be found. the aim of our study was to evaluate the prevalence of antibodies against insulin (ia) and autoantibodies against glutamic acid decarboxylase (anti-gad), tyrosine phosphatase ia-2 (anti-ia-2), thyroid microsomal peroxidase (anti-tpo) and thyroglobulin (anti-tg) in 55 randomly selected type 1 diabetic patients (34 males, 21 females). mean age of these patients was 39 +/- 12 yrs, m ... | 1999 | 11221467 |