| usefulness of thyroxine and free thyroxine filter paper measurements in neonatal screening for congenital hypothyroidism of preterm babies. | low thyroxine (t4) with normal thyroid stimulating hormone (tsh) is a well known condition in preterm (pt) infants. the establishment of t4 and freet4 (ft4) values in filter paper dried blood spots in pt could provide useful information in the neonatal period. | 2000 | 11002447 |
| evaluation of new tnm lung cancer classification. | a new staging system for lung cancer was proposed by the union internationale contre le cancer in 1997, with stages i and ii subdivided and t3n0m0 assigned as stage iib. we studied the usefulness of this new classification. | 2000 | 11002579 |
| stoichiometry and inter-subunit interaction of the wedge initiation complex, gp10-gp11, of bacteriophage t4. | association of gp10 and gp11 (gp=gene product) is the first step in the assembly pathway of the wedge part of the baseplate of bacteriophage t4. the gp10-gp11 complex constitutes the six tail pins at the corners of the baseplate hexagon on the distal side. the stoichiometry of the subunits, gp10 and gp11, of this complex was determined in combination with sedimentation equilibrium, edman degradation of the complex and sodium dodecylsulfate-polyacrylamide gel electrophoresis (sds-page). from the ... | 2000 | 11004546 |
| chiropractic biophysics digitized radiographic mensuration analysis of the anteroposterior cervicothoracic view: a reliability study. | to investigate the reliability of a radiographic measurement procedure that uses a computer and sonic digitizer to determine projected spinal displacements from an ideal, normal position. | 2000 | 11004652 |
| [assessment of mediastinal tumor involvement (t factor) in lung cancer with transesophageal ultrasonography]. | transesophageal sonography was originally used to assess esophageal-gastric neoplasm. the technique may also be useful in the evaluation of pulmonary neoplasms for possible mediastinal involvement, with regard to both t and n factors. | 2000 | 11004987 |
| outcome of patients with rectal adenocarcinoma and localized pelvic non-nodal metastatic foci. | the aim of this study was to evaluate the outcome of patients with primary rectal adenocarcinoma and soft tissue metastatic foci restricted to the pelvis and to determine whether this entity, which is considered n1 disease in the american joint committee on cancer staging system, behaves like completely replaced nodal disease or the first sign of m1 disease. the clinical course for patients with this finding is not well-described in the literature. | 2000 | 11005486 |
| serum t3 and t4 concentrations in the adult rats treated with herbicide 2,4-dichlorophenoxyacetic acid. | the influence of sublethal doses of 2,4-dichlorophenoxyacetic acid (2,4-d) on serum t3 and t4 concentrations in hsd cpb: wistar rats of both sexes was studied. the trial was performed on 24 males and females respectively, each divided into three groups of 8 animals (control, groups 1 and 2). aqueous solution of the compound (11 mg/kg body weight--group 1 and 110 mg/kg body weight--group 2) or clean tap water (control group) was used. aliquots of 2.4 ml/kg body weight were administered with a sto ... | 2000 | 11005658 |
| drug therapy for hyperthyroidism in pregnancy: safety issues for mother and fetus. | hyperthyroidism (thyrotoxicosis) in pregnancy and the child bearing years is usually attributable to graves' disease. this is an autoimmune condition in which thyroid-stimulating immunoglobulins (tsi) cause hyperthyroidism. as a rule, pregnancy complicates the management of hyperthyroidism, rather than vice versa. however, patients who remain thyrotoxic during pregnancy are at increased risk of maternal and fetal complications, particularly miscarriage and stillbirth. therefore, bodyweight loss, ... | 2000 | 11005705 |
| cortical potentials associated with voluntary mandibular movements. | movement-related cortical potentials (mrcps) are negative potentials over the scalp, which gradually increase prior to voluntary movements, and might be applied to elucidate the cortical efferent function of the mandibular movements. we compared the mrcps accompanying various mandibular movements to study the motor control mechanism underlying these movements. electroencephalograms (eegs) were recorded from 11 electrodes placed over the scalp (f3, fz, f4, t3, c3, cz, c4, t4, p3, pz, and p4), acc ... | 2000 | 11005737 |
| sound velocity measurements of nuclear-ordered solid 3he along the melting curve. | we have measured the temperature dependence of the 10.98 mhz longitudinal sound velocity of solid 3he in the nuclear-ordered u2d2 phase and in the paramagnetic phase along the melting curve. the temperature dependence of the sound was attributed to the contributions from the nuclear spin system and the molar volume change along the melting curve. the sound velocity increased with temperature as t4 in the u2d2 phase and the sound anisotropy due to the exchange interaction was found to be about 10 ... | 2000 | 11005982 |
| [median sagittal mandibulotomy in head-neck tumors]. | histologically proven radical resections are the goal in patients with head and neck cancer because of improved survival rates. the frequency of histologically radical resections using the median mandibulotomy and the morbidity of this approach for tumors of the oropharynx and the parapharyngeal space are described in a patients series. | 2000 | 11006914 |
| kinetic characterization of outer-ring deiodinase activity (ord) in the liver, gill and retina of the killifish fundulus heteroclitus. | conversion of t4 to t3 is the first step in th action and deiodinases are the major determinants of th tissue availability and disposal. we here report the kinetic characterization of the outer-ring deiodinating (ord) enzymes in the liver, gill and retina of sea water-adapted killifish, by using both rt3 and t4 as substrates. in liver, by using rt3, we detected a high km (84 nm) and a low km (1.3 nm) component with kinetic characteristics similar to mammalian deiodinases di and dii. in contrast, ... | 2000 | 11007170 |
| synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase and several ligases. | the findings presented here originally arose from the suggestion that the synthesis of dinucleoside polyphosphates (np(n)n) may be a general process involving enzyme ligases catalyzing the transfer of a nucleotidyl moiety via nucleotidyl-containing intermediates, with release of pyrophosphate. within this context, the characteristics of the following enzymes are presented. firefly luciferase (ec 1.12. 13.7), an oxidoreductase with characteristics of a ligase, synthesizes a variety of (di)nucleos ... | 2000 | 11007993 |
| correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of acanthamoeba spp. | eleven acanthamoeba isolates, obtained from acanthamoeba keratitis patients, from contact lens cases of non-acanthamoeba keratitis patients, from asymptomatic individuals, from necrotic tissue, and from tap water and two reference strains were investigated by morphological, molecular biological, and physiological means in order to discriminate clinically relevant and nonrelevant isolates. all clinically relevant isolates showed acanthamoeba sp. group ii morphology. 18s ribosomal dna sequencing r ... | 2000 | 11010891 |
| expression of thyroid receptor isoforms in the human fetal central nervous system and the effects of intrauterine growth restriction. | congenital hypothyroidism is known to be associated with mental retardation which, if recognized promptly, is largely prevented by thyroid hormone replacement. intrauterine growth restriction (iugr) is a major cause of perinatal mortality and morbidity, and is also associated with neurodevelopmental delay. fetuses with iugr have reduced circulating concentrations of free thyroxine (t4) and free triiodothyronine (t3), leading to the hypothesis that a reduction in the tissue effects of thyroid hor ... | 2000 | 11012572 |
| functional connections between the suprachiasmatic nucleus and the thyroid gland as revealed by lesioning and viral tracing techniques in the rat. | frequent blood sampling via intraatrial cannula revealed daily rhythms of tsh and thyroid hormones in both male and female wistar rats. thermic ablation of the biological clock, i.e. the suprachiasmatic nucleus (scn), eliminated the diurnal peak in circulating tsh and thyroid hormones. in addition, scn lesions produced a clear decrease of 24-h mean t4 concentrations. a more pronounced effect of scn-lesions on thyroid hormones, as opposed to tsh, suggested routes of scn control additional to the ... | 2000 | 11014240 |
| growth hormone increases connexin-43 expression in the cerebral cortex and hypothalamus. | several studies indicate that systemic gh influences various brain functions. connexin-43 forms gap junctions that mediate intercellular communication and establish the astroglial syncytium. we investigated the effects of peripheral administration of bovine gh (bgh) and recombinant human insulin-like growth factor i (rhigf-i) on the expression of connexin-43 in the rat brain. hypophysectomized female sprague dawley rats were substituted with cortisol (400 microg/kg x day) and l-t4 (10 microg/kg ... | 2000 | 11014245 |
| the somatostatin analogue octreotide modulates iodothyronine deiodinase activity and pituitary neuromedin b. | somatostatin inhibits growth hormone and thyrotropin (tsh) secretion. it also enhances the inhibitory effect of thyroid hormone (th) on tsh by poorly understood mechanisms. we investigated the acute effect of the long-acting somatostatin analogue, octreotide (oct), on anterior pituitary type 1 (d1) and 2 (d2) deiodinase activity, on liver d1, and on pituitary content of neuromedin b (nb), an autocrine inhibitor of tsh secretion, which is positively regulated by thyroid hormones. euthyroid or hyp ... | 2000 | 11014308 |
| sensitive thyroid-stimulating antibody assay in whole serum containing five percent polyethylene glycol using porcine thyroid cells. | previously we reported that the amounts of cyclic adenosine monophosphate (camp) production by polyethylene glycol (peg) 22.5% precipitated fraction (pf) (crude immunoglobulin g [igg]) from thyroid-stimulating antibody (tsab)-positive serum were higher than those by peg 12.5% pf, and that peg (5%) augmented affinity purified tsab-igg-stimulated camp production in porcine thyroid cells (ptc) assay. in the present work, we studied sensitive tsab assay using whole sera in the presence of high peg c ... | 2000 | 11014309 |
| atypical hypothyroidism and the very low birthweight infant. | results of thyroid screening tests were examined retrospectively on 311,282 infants born in massachusetts from january 1, 1993 to december 31, 1996. during this period, 118 infants were found to have typical hypothyroidism, characterized by a low thyroxine (t4) and an elevated thyrotropin (tsh) on the initial newborn-screening specimen. of these, 98 were normal birthweight (nbw, > or = 2,500 g), 9 were low birthweight (lbw, 1,501-2,499 g), and 11 were very low birthweight (vlbw, < or = 1,500 g). ... | 2000 | 11014314 |
| effect of subclinical hypothyroidism on body fluid compartments. | the present study was aimed to assess the effects of subclinical hypothyroidism on body composition (bc). | 2000 | 11014384 |
| thermodynamic and structural studies of cavity formation in proteins suggest that loss of packing interactions rather than the hydrophobic effect dominates the observed energetics. | the hydrophobic effect is widely believed to be an important determinant of protein stability. however, it is difficult to obtain unambiguous experimental estimates of the contribution of the hydrophobic driving force to the overall free energy of folding. thermodynamic and structural studies of large to small substitutions in proteins are the most direct method of measuring this contribution. we have substituted the buried residue phe8 in rnase s with alanine, methionine, and norleucine. bindin ... | 2000 | 11015216 |
| impact of lead exposure on pituitary-thyroid axis in humans. | thyroid function tests (serum levels of thyroxine-t4, triiodothyronine-t3 and thyroid stimulating hormone-tsh) were performed in fifty-eight men (mean age: 31.7 +/- 10.6 years; mean duration of lead exposure: 156.9 +/- 122.7 months). these subjects were exposed to lead either as petrol pump workers or automobile mechanics. the mean whole blood lead (pb-b) levels were 2.49 +/- 0.45 micromole/l (51.90 +/- 9.40 microg/dl) in the lead exposed workers and were approximately 5 times higher than in the ... | 2000 | 11016408 |
| radical surgery for gallbladder cancer: current options. | gallbladder carcinoma is the most common malignancy of the biliary tract. there are still many controversies regarding the type of curative surgical treatment for each stage of the disease. the staging system used is the tnm classification of the international union against cancer. different patterns of spread characterize gallbladder cancer but the two main types are direct invasion and lymph node metastases; since only the depth of invasion can be easily recognized by imaging techniques, it be ... | 2000 | 11016462 |
| the success rate of identification of the sentinel lymph node in breast cancer: our training series. | a new phase of breast conserving surgery has started very recently, aimed at eliminating axillary dissection in node-negative patients by using the sentinel lymph node (sn) technique. between november 1998 and january 2000 we performed 151 operations for breast cancer on 145 patients. we performed axillary lymphoscintigraphy using 99tc-labeled human serum albumin microcolloidal particles injected subdermally in 50 patients who met our selection criteria. in this series we focused on the success ... | 2000 | 11016712 |
| cisplatin-epirubicin-paclitaxel weekly administration with g-csf support in advanced breast cancer. a southern italy cooperative oncology group (sicog) phase ii study. | it has been shown in vitro that both cisplatin and epirubicin increase the antitumor activity of paclitaxel. weekly administration could give a substantial improvement in the therapeutic index of cisplatin and paclitaxel. this study was aimed at defining the antitumor activity of a weekly cisplatin-epirubicin-paclitaxel (pet) administration in locally advanced or metastatic breast cancer patients. | 2000 | 11016746 |
| enzymatic mutation detection in the p53 gene. | the enzymatic mutation detection (emd) assay uses the bacteriophage resolvase t4 endonuclease vii, which cleaves preformed heteroduplex molecules at mismatch sites, forming two shorter fragments that can be resolved by gel electrophoresis. the method can be used to detect single and multiple base changes, as well as insertions and deletions. | 2000 | 11017933 |
| thyroid stimulating hormone levels in cord blood are not influenced by non-thyroidal mothers' diseases. | screening programs not only offer the opportunity to trace and treat almost all cases of congenital hypothyroidism but also mean large savings to the health system. however, carefully planned strategies are necessary to extend their benefits and reduce costs. | 2000 | 11018848 |
| [advanced gastric cancer: the findings of delayed phase dynamic ct and radiologic-histopathologic correlation]. | the aim of this study was to describe delayed phase dynamic ct findings of advanced (t2-t4) gastric cancer and to correlate with histopathologic findings. | 2000 | 11019578 |
| reduced global genomic repair of ultraviolet light-induced cyclobutane pyrimidine dimers in simian virus 40-transformed human cells. | the p53 tumor-suppressor gene has been implicated in the inducible activation of excision repair of ultraviolet (uv)-induced cyclobutane pyrimidine dimers (cpds) in human cells. because the large t antigen (ltag) of the simian virus 40 (sv40) binds p53 protein and can interfere with its function, it was of interest to study dna repair in normal human fibroblasts that had been transformed by sv40 compared with that in their nontransformed parental counterparts and to determine whether such transf ... | 2000 | 11020243 |
| high-dose-rate interstitial brachytherapy as a monotherapy for localized prostate cancer: treatment description and preliminary results of a phase i/ii clinical trial. | to improve results for localized prostate cancer, a prospective clinical trial of hyperfractionated iridium-192 high-dose-rate (hdr) brachytherapy as a monotherapy was initiated. | 2000 | 11020563 |
| involved-field radiotherapy alone for early-stage non-small-cell lung cancer. | to describe the outcome of involved-field radiotherapy in patients with early-stage non-small-cell lung cancer (nsclc). | 2000 | 11020567 |
| post-partum thyroiditis in south brazil presenting as thyrotoxicosis: prevalence and risk factors. | the prevalence of post-partum thyroiditis (ppt) has been reported in several countries (1.9 to 16.7%) but is not known in brazil. several factors have been associated to its development, such as a female sex of the newborn, ppt in a previous pregnancy, a family history of thyroid disease and cigarette smoking. to investigate the prevalence of ppt and its risk factors in a southern brazilian city, a three-cross-sectional observation study was performed. ppt was diagnosed in 14/284 subjects (5.3%) ... | 2000 | 11021764 |
| t4 early promoter strength probed in vivo with unribosylated and adp-ribosylated escherichia coli rna polymerase: a mutation analysis. | the consensus sequence of t4 early promoters differs in length, sequence and degree of conservation from that of escherichia coli sigma(70) promoters. the enzyme interacting with these promoters, and transcribing the t4 genome, is native host rna polymerase, which is increasingly modified by the phage-encoded adp-ribosyltransferase, alt. t4 early transcription is a very active process, possibly out-competing host transcription. the much stronger t4 promoters enhance viral transcription by a fact ... | 2000 | 11021939 |
| effect of combined treatment with calcitonin on bone densitometry of patients with treated hypothyroidism. | thyroid hormones (th) may affect bone metabolism and turnover, inducing a loss of bone mass among hyperthyroid and in hypothyroid patients under hormone replacement treatment. thyroid dysfunction leads to changes in the dynamics of parathyroid hormone (pth) and calcitonin (ct) secretion. | 2000 | 11022359 |
| eus in preoperative staging of pancreatic cancer. | endoscopic ultrasound (eus) is believed to be highly accurate in the local (t) and nodal (n) staging of pancreatic cancer. however, there are scant data concerning the predictive value of eus for resectability of pancreatic adenocarcinoma. this study was performed to determine the accuracy of tnm staging by eus in patients with pancreatic adenocarcinoma and to evaluate the role of preoperative tnm staging by eus for determining resectability in patients with pancreatic adenocarcinoma. | 2000 | 11023561 |
| exhaustive mutagenesis in silico: multicoordinate free energy calculations on proteins and peptides. | we have extended and applied a multicoordinate free energy method, chemical monte carlo/molecular dynamics (cmc/md), to calculate the relative free energies of different amino acid side-chains. cmc/md allows the calculation of the relative free energies for many chemical species from a single free energy calculation. we have previously shown its utility in host:guest chemistry (pitera and kollman, j am chem soc 1998;120:7557-7567)1 and ligand design (eriksson et al., j med chem 1999;42:868-881)2 ... | 2000 | 11025549 |
| case series: brief parent-child group therapy for childhood anxiety disorders using a manual-based cognitive-behavioral technique. | to report on a brief parent-child group therapy program for children with anxiety disorders. | 2000 | 11026186 |
| psychological functioning of recently bereaved, middle-aged women: the first 13 months. | this study examined the natural course of psychological functioning in recently bereaved middle-aged women. 69 widows were assessed four times (t1-t4) between the period of 4 to 13 mo. after the loss and were compared to a matched nonwidowed group of 57. of the scl-90 feelings of depression, agoraphobic behavior, anxiety, hostility, somatization, feelings of insufficiency, and sleep disorders were heightened at 4 mo. after bereavement compared to the norm group. significantly higher psychologica ... | 2000 | 11026420 |
| flexibility and ligand exchange in a buried cavity mutant of t4 lysozyme studied by multinuclear nmr. | the leu99-->ala mutant of t4 lysozyme contains a large internal cavity in the core of its c-terminal domain that is capable of reversibly binding small hydrophobic compounds. although the cavity is completely buried, molecules such as benzene or xenon can exchange rapidly in and out. the dynamics of the unliganded protein have been compared to the wild-type protein by measuring the nmr spin relaxation rates of backbone amide and side chain methyl nuclei. many residues surrounding the cavity were ... | 2000 | 11027141 |
| left temporal deficit of p300 in patients with schizophrenia: effects of task. | p300 is often, but not always, observed to be more reduced over left than right temporal lobes in patients with schizophrenia. the possibility that task differences contribute to the inconsistency in the literature was explored in this study. erps were collected from 17 right-handed men with schizophrenia (dsm-iiir) and 11 right-handed healthy male community controls, performing three auditory oddball tasks - respond to a target tone by: (1) counting; (2) pressing a response button with the righ ... | 2000 | 11027795 |
| hyperthyroidism in children. | this study was done to characterize the clinical features, laboratory parameters and response to therapy and outcome of childhood hyperthyroidism. the evaluation included history, examination, laboratory investigations: serum t3, t4, tsh, free t3, free t4 by ria or immunochemiluminescence (ic), antithyroid antibodies by standard techniques, bone age (ba) by greulich and pyle's method, clinical and laboratory response to treatment, and follow-up of 15 children with hyperthyroidism seen in past ei ... | 2000 | 11028118 |
| epidural analgesia-anaesthesia in obstetrics. | epidural analgesia is the most effective and innocuous technique for obstetrics. pain relief is its main indication but maternal diseases that might be decompensated by labour and delivery are also accepted indications. low doses of long-acting local anaesthetics alone or in combination with low doses of fentanyl or sufentanil provide good quality analgesia and are safe for mother and fetus. test doses in parturients lack sufficient specificity and sensitivity for detecting inadvertent intravasc ... | 2000 | 11029122 |
| ballotability of cuff to confirm the correct intratracheal position of the endotracheal tube in the intensive care unit. | the cuff ballotability method was used in 120 adult patients to confirm the correct depth of insertion of the endotracheal tube after tracheal intubation. the correct tube position was assumed when the cuff of the endotracheal tube could be felt to distend over the suprasternal notch when the pilot balloon was squeezed and the pilot balloon was felt to distend when pressure was applied over the suprasternal notch. chest radiography was performed later to confirm the position of the endotracheal ... | 2000 | 11029127 |
| characterization of the distal tail fiber locus and determination of the receptor for phage ar1, which specifically infects escherichia coli o157:h7. | phage ar1 is similar to phage t4 in several essential genes but differs in host range. ar1 infects various isolates of escherichia coli o157:h7 but does not infect k-12 strains that are commonly infected by t4. we report here the determinants that confer this infection specificity. in t-even phages, gp37 and gp38 are components of the tail fiber that are critical for phage-host interaction. the counterparts in ar1 may be similarly important and, therefore, were characterized. the ar1 gp37 has a ... | 2000 | 11029414 |
| oral perception in tongue thrust and other oral habits. | oral stereognosis is the ability of the mouth to recognize shape and texture. oral shape recognition is sensitive to repetition and to topical mucosal anesthesia. age, upper and lower arch perimeter, and labiolingual dysfunction also interact with oral stereognosis. the purpose of this investigation was to define the influence of age, arch size, and oral dysfunction on oral stereognosis when submitted to repeated trials. fifty subjects were selected before orthodontic treatment. each subject und ... | 2000 | 11029733 |
| evaluation of treatment and posttreatment changes of protraction facemask treatment using the par index. | the purpose of this study was to use the peer assesment rating (par) index score to evaluate the treatment and posttreatment changes of class iii patients treated by protraction facemask. the sample consisted of 20 chinese children, 6 to 11 years old, with class iii skeletal malocclusion who had been treated with maxillary expansion and a protraction facemask. the average treatment time was 8.2 months, followed by 1 year of retention with a class iii functional appliance. study casts were taken ... | 2000 | 11029737 |
| low dose oral iodized oil for control of iodine deficiency in children. | in areas where iodized salt is not available, oral iodized oil is often used to correct i deficiency despite a lack of consensus on the optimal dose or duration of effect, particularly in children, a main target group. annual doses ranging from 400 to 1000 mg have been advocated for school-age children. because lower doses of iodized oil have been shown to be effective in treating i deficiency in adults, the aim of this study was to evaluate the efficacy and safety of a low dose of oral iodized ... | 2000 | 11029963 |
| nmr structure of oxidized glutaredoxin 3 from escherichia coli. | a high precision nmr structure of oxidized glutaredoxin 3 [c65y] from escherichia coli has been determined. the conformation of the active site including the disulphide bridge is highly similar to those in glutaredoxins from pig liver and t4 phage. a comparison with the previously determined structure of glutaredoxin 3 [c14s, c65y] in a complex with glutathione reveals conformational changes between the free and substrate-bound form which includes the sidechain of the conserved, active site tyro ... | 2000 | 11031118 |
| lateralized p3 deficit in schizotypal personality disorder. | reduced, left-lateralized p3 amplitude has been reported in several studies focusing on electrophysiologic function in schizophrenia. also, several lines of evidence suggest a similarity between schizophrenia and schizotypal personality disorder (spd). this study was undertaken to determine the replicability of our previous finding of a left-lateralized p3 amplitude deficit in spd. | 2000 | 11032982 |
| the value of interleukin-12 as an activity marker of pulmonary sarcoidosis. | sarcoidosis is characterized by hyperactivity of t-helper lymphocytes and recent studies showed that they were mainly th1 cells. il-12 is a major cytokine inducing th1 differentiation of naive t cells. this study was performed to test whether il-12 can be a marker for disease activity and possibly a prognosis in sarcoidosis. | 2000 | 11033843 |
| advanced squamous cell carcinoma of the base of the tongue treated with surgery and post-operative radiotherapy. | early stage squamous cell carcinoma of the base of the tongue has been successfully treated with radiotherapy and brachytherapy. however, the vast majority of these tumours seen in western europe are already at an advanced stage. medical records of 79 patients with squamous cell carcinoma of the base of the tongue treated between 1980 and 1994 were examined. | 2000 | 11034805 |
| appraisal of treatment strategy by staging laparoscopy for locally advanced gastric cancer. | more accurate preoperative staging is necessary to determine the treatment strategy for locally advanced gastric cancer. thirty-two patients with t3 or t4 gastric cancer expected to undergo curative resection based on conventional examinations underwent staging laparoscopy. the disease stages determined were compared with those obtained by conventional methods. the discrepancy rate of disease staging was 16 of 32 (50.0%), with down-staging in 5 of 32 (15.6%) and up-staging in 11 of 32 (34.4%). o ... | 2000 | 11036293 |
| markers of cell lineage, differentiation and activation. | the most widespread use of cd markers is in the determination of cell lineage and sublineage. for example, t cells are identified by the expression of cd3 (reviewed in this issue of cd corner). a mature t cell may belong to the t4 subset, in which case it will express cd4. similarly, there are markers for other cell populations and sub-populations. within the lineages, it is helpful to distinguish cells at different stages of differentiation and activation. differentiation status is particularly ... | 2000 | 11037056 |
| external beam radiation followed by planned neck dissection and brachytherapy for base of tongue squamous cell carcinoma. | surgical resection of tongue base cancer can leave the patient with significant functional deficits. other therapies, such as external beam radiation followed by neck dissection and radiation implants, have shown equal tumor control with good functional outcome. | 2000 | 11037816 |
| familial papillary thyroid carcinoma: genetics, criteria for diagnosis, clinical features, and surgical treatment. | hereditable predisposition to papillary thyroid carcinoma (ptc) and multinodular goiter (mng) without evidence of an association with other malignancies as a distinct entity was recognized only recently. a meta-review of the literature on familial ptc (fptc) was undertaken, and characteristics of families with frequent occurrence of ptc or mng (or both) were summarized. a database on thyroid cancer patients maintained in our institution was searched for potential fptc families. clinical examinat ... | 2000 | 11038215 |
| [long-term results of preoperative chemotherapy for operable breast cancer]. | to evaluate the long-term therapeutic results of preoperative chemotherapy for operable breast cancer. | 1997 | 11038758 |
| effects of anesthesia, surgery, and intravenous administration of fluids on plasma antidiuretic hormone concentrations in healthy dogs. | to evaluate effects of anesthesia, surgery, and intravenous administration of fluids on plasma concentrations of antidiuretic hormone (adh), concentration of total solids (ts), pcv, arterial blood pressure (bp), plasma osmolality, and urine output in healthy dogs. | 2000 | 11039560 |
| hypopharyngeal squamous cell carcinoma--treatment results in 138 consecutively admitted patients. | the aim of this study was to evaluate the results of the initial and salvage treatment for hypopharyngeal carcinoma. the study was conducted in 1963 to 1991 and included 138 patients (38 females (28%) and 100 males (73%)). most of the tumours originated in the piriform sinus (86%). tumour stage distribution was t1: 20%, t2: 27%, t3: 37% and t4: 17% and nodal stage distribution was n0: 45%, n1: 25%, n2: 10%, and n3: 20%. primary treatment was delivered with curative intent in 124 out of 138 cases ... | 2000 | 11041117 |
| thyroid hormone stimulates na, k-atpase gene expression in the hemodynamically unloaded heterotopically transplanted rat heart. | regulation of myocardial na, k-atpase gene expression by thyroid hormone was investigated in the heterotopically transplanted rat heart to distinguish the direct effects of the hormone on the heart from effects secondary to increased hemodynamic workload. in this model, the transplanted heart is histologically normal and spontaneously beating, but hemodynamically unloaded. three days after transplantation, relative contents of ventricular na, k-atpase alpha2- and beta1-mrnas and alpha1- and alph ... | 2000 | 11041452 |
| changes in lipoprotein(a) levels in overt and subclinical hypothyroidism before and during treatment. | the aim of this prospective, follow-up study was to examine the influence of overt hypothyroidism (ohp) and subclinical (shp), before and during thyroxine (t4) treatment, on lipoprotein(a) [lp(a)], other lipoproteins, and apolipoproteins. twenty-four patients (17 females, 7 males) with ohp, aged 54 +/- 11.1 years (group a) and 23 patients (females) with shp aged 50.1 +/- 13.2 years (group b) were evaluated and compared to 34 and 38 controls, respectively. all patients received t4 therapy in a st ... | 2000 | 11041458 |
| dietary genistein inactivates rat thyroid peroxidase in vivo without an apparent hypothyroid effect. | biological effects of genistein are currently under investigation by the national toxicology program because of widespread and increasing soy consumption by humans and evidence for modulation of endocrine function. rats were exposed to genistein aglycone in soy-free feed fortified at 0, 5, 100, and 500 ppm starting in utero through 20 weeks. thyroid glands and serum were analyzed for total genistein (aglycone + conjugates) using hplc with electrospray mass spectrometric detection. microsomal thy ... | 2000 | 11042097 |
| identification and characterization of three novel beta 1,3-n-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family. | we have isolated three types of cdnas encoding novel beta1,3-n-acetylglucosaminyltransferases (designated beta3gn-t2, -t3, and -t4) from human gastric mucosa and the neuroblastoma cell line sk-n-mc. these enzymes are predicted to be type 2 transmembrane proteins of 397, 372, and 378 amino acids, respectively. they share motifs conserved among members of the beta1,3-galactosyltransferase family and a beta1,3-n-acetylglucosaminyltransferase (designated beta3gn-t1), but show no structural similarit ... | 2001 | 11042166 |
| glycosyltransferase structure and mechanism. | the high-resolution x-ray crystal structures of a new form of bacteriophage t4 beta-glucosyltransferase, escherichia coli murg, bacillus subtilis spsa, bovine beta-1,4-galactosyltransferase 1 and rabbit n-acetylglucosaminyltransferase i have now been solved. these glycosyltransferase structures have provided the first detailed view of the structural basis of catalysis, as well as new insight into glycosyltransferase classification. | 2000 | 11042447 |
| structure and folding of bacteriophage t4 gene product 9 triggering infection. ii. study of conformational changes of gene product 9 mutants using monoclonal antibodies. | gene product 9 (gp9) of bacteriophage t4, whose spatial structure we have recently solved to 2.3 a resolution, is a convenient model for studying the folding and oligomerization mechanisms of complex proteins. the gp9 polypeptide chain consists of 288 amino acids forming three domains. three monomers, packed in parallel, assemble to a functionally active protein. the main aim of this work was to study conformational changes and trimerization of gp9 deletion mutants using monoclonal antibodies (m ... | 2000 | 11042500 |
| evaluation of the validity of the 1997 international union against cancer tnm classification of major salivary gland carcinoma. | the tnm classification (international union against cancer) of salivary gland carcinoma was revised in 1997. in this study, the authors have evaluated the validity of this new tnm classification and clinical staging in 1683 patients with parotid gland carcinoma. | 2000 | 11042558 |
| masses of the tongue and floor of the mouth: findings on magnetic resonance imaging. | the purpose of this study was to determine the value of mr imaging for the demonstration of masses in the tongue and floor of the mouth. nine patients were prospectively examined with mr imaging after physical examination. imaging protocol included t2 and contrast-enhanced and non-contrast-enhanced t1-weighted turbo spin-echo sequences, and the findings were compared with surgical and histopathological results. histopathological examination revealed four squamous cell carcinomas, one adenoid cys ... | 2000 | 11044946 |
| severity of hyperemesis gravidarum correlates with serum levels of reverse t3. | to investigate the possible physiological relevance of extra-thyroidal production of reverse t3 (rt3) in hyperemesis gravidarum, measurements of serum rt3, free t3 (ft3), free t4, (ft4), and nonesterified fatty acids (nefa) were correlated with weight loss of hyperemetic women. all the thyroid hormones, nefas and weight loss were significantly higher in hyperemesis gravidarum than in control subjects, and also higher than in those with milder symptoms of morning sickness (p < 0.05). elevations o ... | 2000 | 11045323 |
| [evaluation of new tnm classification for lung cancer, especially t3n0m0, stage iiia, stage iiib, and pm]. | the purpose of this study was to evaluate the results of new tnm staging system for lung cancer in 1997, especially t3n0m0, stage iiia, stage iiib, and pm. five-year survival rates of the patients with stage iiia and stage iiib were 16% and 18% respectively (ns). five-year survival rates of patients with t3n1m0, t1n2m0, t2n2m0, and t3n2m0 were 40%, 28%, 15%, and 3%, respectively. the prognosis of t3n2m0 was significantly worse than that of t3n1m0, t1n2m0, and t2n2m0. five-year survival rates of ... | 2000 | 11048439 |
| [assessment of the new tnm classification for resected lung cancer]. | to evaluate the revised tnm classification, we investigated the prognoses of 552 consecutive patients who had resection of non-small-cell lung cancer between april 1982 and march 1996. according to the new classification, the 5-year survival rate was 76.9% for stage i a, 57.2% for stage i b (i a versus i b, p < 0.0005), 47.7% for stage iia, 49.8% for stage iib, 18.6% for stage iiia (iib versus iiia, p = 0.005), 16.7% for stage iiib, and 7.9% for stage iv (iiib versus iv, p = 0.02). especially fo ... | 2000 | 11048441 |
| [the validity and problems of a new staging system for lung cancer from the point of view of surgical results]. | the validity and problems of the new staging system for resected lung cancer were assessed. in the new staging system for primary lung cancer, stage i is divided into two groups (a and b) on the basis of the tumor diameter. the boundary is 30 mm in maximum diameter of the tumor. on the other hand, intrapulmonary satellite nodules in the resected lung (pm1) are considered as t4. this means that a patient with pm1 is classified as stage iiib or iv. the five-year cumulative survival rates of 191 co ... | 2000 | 11048444 |
| atrial natriuretic peptide and thyroid hormones' relation to plasma and heart calcium and magnesium concentrations of wistar rats exposed to cold and hot ambients. | plasma anp (atrial natriuretic peptide), thyroid hormones, and calcium and magnesium levels as well as heart tissue calcium and magnesium concentrations were determined in male wistar rats after exposure of 114 rats at low temperature (4 degrees c) and 95 rats at high temperature (35-36 degrees c) for 28 d. plasma anp, triiodothyronine (t3), thyroxine (t4), thyroid-stimulating hormone (tsh), free t3, and free t4 were estimated by radioimmunoassay, and plasma and heart tissue levels of ca and mg ... | 2000 | 11049208 |
| role of presympathetic c1 neurons in the sympatholytic and hypotensive effects of clonidine in rats. | the rostral ventrolateral medulla (rvlm) may play an important role in the sympatholytic and hypotensive effects of clonidine. the present study examined which type of presympathetic rvlm neuron is inhibited by clonidine, and whether the adrenergic presympathetic rvlm neurons are essential for clonidine-induced sympathoinhibition. in chloralose-anesthetized and ventilated rats, clonidine (10 microg/kg iv) decreased arterial pressure (116 +/- 6 to 84 +/- 2 mmhg) and splanchnic nerve activity (93 ... | 2000 | 11049859 |
| the importance of a mobile loop in regulating chaperonin/ co-chaperonin interaction: humans versus escherichia coli. | chaperonins are universally conserved proteins that nonspecifically facilitate the folding of a wide spectrum of proteins. while bacterial groel is functionally promiscuous with various co-chaperonin partners, its human homologue, hsp60 functions specifically with its co-chaperonin partner, hsp10, and not with other co-chaperonins, such as the bacterial groes or bacteriophage t4-encoded gp31. co-chaperonin interaction with chaperonin is mediated by the co-chaperonin mobile loop that folds into a ... | 2001 | 11050098 |
| staging of rectal cancer by double-contrast mr imaging using the rectally administered superparamagnetic iron oxide contrast agent ferristene and iv gadodiamide injection: results of a multicenter phase ii trial. | the aim of this study was to assess the accuracy of double-contrast magnetic resonance imaging (mri) with rectal application of the superparamagnetic iron oxide contrast agent (spio) ferristene and iv gadodiamide for preoperative staging of rectal cancer. in a randomized phase ii dose-ranging trial, 113 patients were studied preoperatively with one of four different formulations of ferristene (abdoscan) as an enema before mri. t1-weighted spin-echo (t1w se) and t2w turbo spin-echo (tse) single-c ... | 2000 | 11050634 |
| reversion of arge3 ochre strain escherichia coli ab1157 as a tool for studying the stationary-phase (adaptive) mutations. | adaptive (starvation-associated) mutations occur in non-dividing cells and allow growth under the selective conditions imposed. we developed a new method for the determination of adaptive mutations in escherichia coli. the system involves reversion to prototrophy of the arge3oc mutation and was tested on ab1157 strains mutated in the mutt and/or muty genes. the bacteria that mutated adaptively grow into colonies on minimal medium plates devoid of arginine (starvation conditions) when incubated l ... | 2000 | 11051211 |
| esophageal dilation for endosonographic evaluation of malignant esophageal strictures is safe and effective. | endoscopic ultrasound (eus) is accepted as the most accurate modality for t- and n-staging of esophageal cancer, but some malignant strictures prevent passage of the echoendoscope beyond the level of the tumor. this incomplete evaluation may decrease staging accuracy. previous studies have yielded conflicting results regarding the safety and efficacy of esophageal dilation for eus. | 2000 | 11051353 |
| [conservative treatment of adenocarcinomas of the anorectal junction by preoperative radiotherapy and intersphincteral resection]. | adenocarcinomas of the anorectal junction, especially t3 lesions, are usually treated by abdominoperineal resection. the aim of this study was to evaluate oncologic and functional results following conservative radiosurgical treatment of cancers of the anorectal junction. | 2000 | 11051690 |
| increased plasma levels of corticosterone and prolactin and decreased t3 and t4 levels in short-term prehepatic portal hypertension in rats. | corticosterone, t3, t4, and prolactin serum concentrations at 24 hr (n = 10), 15 days (n = 10), and 45 days (n = 10) of postoperative (postop) evolution were assayed to study the neuroendocrine response to portal hypertension. a triple stenosing ligature of the portal vein was used as the surgical technique of portal hypertension. this technique does not produce mortality and causes a decrease in the serum concentrations of t3 (0.043 +/- 0.009 vs 0.55 +/- 0.08 ng/ml) and t4 (3.93 +/- 0.55 vs 4.6 ... | 2000 | 11052333 |
| assessment of sex hormone-binding globulin and osteocalcin in patients undergoing coronary artery bypass graft surgery. | to determine sex hormone-binding globulin (shbg) and osteocalcin (oc) levels in patients undergoing coronary artery bypass graft surgery to clarify the status of peripheral thyroid metabolism and to correlate shbg and oc with thyroid hormones and adverse postoperative events. | 2000 | 11052436 |
| radiology of the retropharyngeal space. | the retropharyngeal space extends from the skull base to the t4 vertebral level and contains the retropharyngeal nodes superiorly and fatty tissue elsewhere. this space is important as it is a potential route for the spread of infection and malignancy. this pictorial essay outlines the anatomy of the retropharyngeal space and illustrates the various disease processes that may be seen in region.chong, v. f. h., fan, y. f. (2000). clinical radiology55, 740-748. | 2000 | 11052873 |
| domain effects on the dna-interactive properties of bacteriophage t4 gene 32 protein. | bacteriophage t4 gene 32 protein, a model for single-strand specific nucleic acid-binding proteins, consists of three structurally and functionally distinct domains. we have studied the effects of the n and c domains on the protein structure and its nucleic acid-interactive properties. although the presence of the c domain decreases the proteolytic susceptibility of the core (central) domain, quenching of the core tryptophan fluorescence by iodide is unaltered by the presence of the terminal dom ... | 2001 | 11053417 |
| the effect of atrazine on puberty in male wistar rats: an evaluation in the protocol for the assessment of pubertal development and thyroid function. | since atrazine (atr), a chlorotriazine herbicide, has been shown previously to alter the secretion of luteinizing hormone (lh) and prolactin (prl) through a direct effect on the central nervous system (cns), we hypothesized that exposure to atr in the edstac male pubertal protocol (juvenile to peripubertal) would alter the development of the male rat reproductive system. we dosed intact male wistar rats from postnatal day (pnd) 23 to 53 and examined several reproductive endpoints. atr (0, 12.5, ... | 2000 | 11053540 |
| bilateral open treatment of spontaneous pneumothorax: a new access. | a new technique for bilateral apical bullectomy and pleurectomy via axillary minithoracotomy and transmediastinal access to the contralateral side, was used in 13 patients with bilateral apical blebs and/or pneumothorax. the contralateral space is reached at the posterior superior mediastinum, passing between the first thoracic vertebral bodies (t1-t4) and the oesophagus. the contralateral lung apex is then pulled into the thoracotomy side and apical bullectomy carried out by linear stapler. the ... | 2000 | 11053826 |
| comparison of cyfra 21-1 and scc assays in head and neck tumours. | patients with head and neck tumours (hnt) have a high risk of early locoregional relapse that is difficult to diagnose. this study evaluated the usefulness of the serum cyfra 21-1 assay compared to squamous cell carcinoma antigen (scc) assay for monitoring such patients. three hundred and twelve hnt patients, including 204 newly diagnosed patients, were followed up for a median of 446 days with serial serum assays for scc and cyfra 21-1. untreated patients showed scc and cyfra 21-1 serum levels ... | 2001 | 11054024 |
| carcinoma of the maxillary antrum: a retrospective analysis of 110 cases. | cancer of the maxillary antrum is a rare disease with a variety of treatment options. the present study was undertaken to review the outcome of patients with carcinoma of the maxillary antrum managed at a single institution. | 2000 | 11054520 |
| hypothyroidism and depression: a therapeutic challenge. | to describe a patient with longstanding depression and hypothyroidism who had marked mood improvement only after triiodothyronine (t3) was added to her thyroxine (t4) replacement therapy. | 2000 | 11054982 |
| pre-steady state kinetics of bacteriophage t4 dam dna-[n(6)-adenine] methyltransferase: interaction with native (gatc) or modified sites. | the dna methyltransferase of bacteriophage t4 (t4 dam mtase) recognizes the palindromic sequence gatc, and catalyzes transfer of the methyl group from s:-adenosyl-l-methionine (adomet) to the n(6)-position of adenine [generating n(6)-methyladenine and s:-adenosyl-l-homocysteine (adohcy)]. pre-steady state kinetic analysis revealed that the methylation rate constant k(meth) for unmethylated and hemimethylated substrates (0.56 and 0.47 s(-1), respectively) was at least 20-fold larger than the over ... | 2000 | 11058118 |
| formation of cis-diamminedichloroplatinum(ii) 1,2-intrastrand cross-links on dna is flanking-sequence independent. | mapping of cis-diamminedichloroplatinum(ii) (cis-ddp, cisplatin) dna adducts over >3000 nucleotides was carried out using a replication blockage assay. the sites of inhibition of modified t4 dna polymerase, also referred to as stop sites, were analyzed to determine the effects of local sequence context on the distribution of intrastrand cisplatin cross-links. in a 3120 base fragment from replicative form m13mp18 dna containing 24.6% guanine, 25.5% thymine, 26.9% adenine and 23.0% cytosine, 166 i ... | 2000 | 11058123 |
| thyroid hormone changes in males exposed to lead in the buenos aires area (argentina). | the relationship between lead levels in blood (pbb) and hormones t3, t4, t4f and tsh were studied in 75 subjects exposed to lead at work. pbb levels in blood were determined by atomic absorption spectrophotometry and hormones by enzymoimmunoassay. positive and significant correlations among thyroid hormones and tsh vs blood lead level in the ranges 8-50 and 26-50 micro g dl(-1)were found ('r' between 0.304 and 0.621 and 0.431 and 0.619, respectively). at pbb levels between 8 and 26 micro g dl(-1 ... | 2000 | 11058414 |
| the relationship between thyroxine, oestradiol, and postnatal alopecia, with relevance to women's health in general. | post-partum hair loss is possibly due to a reduction in the levels of oestradiol and thyroxine postnatally. alopecia and/or a persistent loss of hair condition postnatally is associated with a group of symptoms (a syndrome), wherein postnatal depression is significant, as a result of physiologically inadequate levels of thyroxine (t4) and oestradiol (e2), secondary to physiological postnatal anterior pituitary dysfunction. using this hypothesis, the author began to apply the same hypothesis to o ... | 2000 | 11058426 |
| beta 1,3-galactosyltransferase beta 3gal-t5 acts on the glcnacbeta 1-->3galbeta 1-->4glcnacbeta 1-->r sugar chains of carcinoembryonic antigen and other n-linked glycoproteins and is down-regulated in colon adenocarcinomas. | we attempted to determine whether beta1,3-galactosyltransferase beta3gal-t5 is involved in the biosynthesis of a specific subset of type 1 chain carbohydrates and expressed in a cancer-associated manner. we transfected chinese hamster ovary (cho) cells expressing fuc-tiii with beta3gal-t cdnas and studied the relevant glycoconjugates formed. beta3gal-t5 directs synthesis of lewis type 1 antigens in cho cells more efficiently than beta3gal-t1, whereas beta3gal-t2, -t3, and -t4 are almost unable t ... | 2001 | 11058588 |
| investigation of the relationship between low apgar scores and early neonatal thyroid function. | the aim of the present study was to assess the effects of low apgar scores on perinatal thyroid function. | 2000 | 11059541 |
| administration of a single low dose of recombinant human thyrotropin significantly enhances thyroid radioiodide uptake in nontoxic nodular goiter. | radioiodine (131i) is increasingly used as treatment for volume reduction of nontoxic, nodular goiter. a high dose of 131i is often needed because of low thyroid radioiodide uptake (raiu). we investigated whether pretreatment with a single, low dose of recombinant human tsh (rhtsh; thyrogen, genzyme transgenics corp.) enhances raiu in 15 patients with nontoxic, nodular goiter (14 women and 1 man; aged 61+/-11 yr). four patients were studied twice, and 1 patient was studied 3 times. raiu was meas ... | 2000 | 11061507 |
| detection of recurrent thyroid cancer by sensitive nested reverse transcription-polymerase chain reaction of thyroglobulin and sodium/iodide symporter messenger ribonucleic acid transcripts in peripheral blood. | to investigate whether circulating thyroglobulin (tg) messenger ribonucleic acid (mrna) and sodium/iodide symporter (nis) mrna transcripts in peripheral blood are valuable in the follow-up of patients with thyroid cancer, we developed highly sensitive nested tg and nis mrna detection assays and compared their accuracy with serum thyroglobulin (stg) and whole body scan with 131i during the monitoring of 34 patients with well differentiated thyroid carcinoma who had undergone total thyroidectomy ( ... | 2000 | 11061512 |
| circulating thyrotropin bioactivity in sporadic central hypothyroidism. | the etiopathogenesis of sporadic central hypothyroidism (ch) involves pituitary and hypothalamic lesions. pituitary ch (pch) implies a diminished number of functioning thyrotropes, accounting for the quantitative impairment of tsh secretion. hypothalamic ch (hch) is characterized by normal or even increased tsh concentrations and qualitative abnormalities of tsh secretion, including a decreased bioactivity of circulating tsh. however, controversy still exists about the actual occurrence of bioin ... | 2000 | 11061514 |
| a novel mutation causing complete thyroxine-binding globulin deficiency (tbg-cd-negev) among the bedouins in southern israel. | t4-binding globulin (tbg) is the major thyroid hormone transport protein in human serum. inherited tbg abnormalities do not usually alter the metabolic status and are transmitted in x-linked inheritance. a high prevalence of complete tbg deficiency (tbg-cd) has been reported among the bedouin population in the negev (southern israel). in this study we report a novel single mutation causing complete tbg deficiency due to a deletion of the last base of codon 38 (exon 1), which led to a frame shift ... | 2000 | 11061524 |
| hashimoto's thyroiditis: countrywide screening of goitrous healthy young girls in postiodization phase in india. | countrywide salt iodization, to prevent nutritional iodine deficiency, has been achieved in india recently. the current study was planned to evaluate the prevalence of goiter and thyroid autoimmunity and assess thyroid functional status in a cohort of 6283 healthy schoolgirls from different parts of the country in the postiodization phase. goitrous girls (n = 1810; 28% of subjects) were investigated for serum t4 and tsh, antithyroid microsomal antibody (tma) and antithyroglobulin antibody (tga), ... | 2000 | 11061541 |
| comparison of a linear miniature ultrasound probe and a radial-scanning echoendoscope in tn staging of esophageal cancer. | endoscopic ultrasonography is a precise method for tn staging of esophageal cancer. we explored the staging properties of a linear miniprobe as compared with a radial-scanning echoendoscope. | 2000 | 11063164 |
| localization of initial lymph node metastasis from carcinoma of the thoracic esophagus. | most surgeons consider esophageal carcinoma with lymph node involvement a systemic disease. however, it is possible that the disease may be localized in the earlier phases of lymphatic metastasis. the distribution of involved lesions in the initial phase of lymph node metastasis has not been thoroughly investigated yet. | 2000 | 11064342 |