| nutritional evaluation of organically grown fodders in lactating murrah buffaloes (bubalus bubalis). | a study was undertaken to evaluate the nutritional response of organically grown diets in buffaloes on nutrients utilization and nutrient efficiency for milk production. for this, ten milch murrah buffaloes of average body weight (490.72 ± 6.65 kg), milk yield (8.13 ± 0.33 kg), and lactation stage (85 ± 5.28 days) were distributed in inorganic (indg()) and organic dietary groups (ordg) with five animals in each. buffaloes of indg and ogdg were fed sorghum hay-berseem fodder-concentrate mixture-b ... | 2013 | 22733348 |
| molecular phylogeny of pakistani riverine buffalo based on genetic variability of mitochondrial cytochrome b gene. | mitochondrial cytochrome b gene is considered to be one of the best markers for breed characterization as well as studying the ancestry in the vertebrates due to its exclusive maternal inheritance. dna fingerprinting by single nucleotide polymorphism is most reliable and widely used molecular technique in modern forensics and is being considered in this study. partial sequencing of 1,061 bp of aforementioned gene from 14580 to 15643 was conducted in two famous pakistani buffalo breeds named nili ... | 2012 | 22718511 |
| in vivo differentiation potential of buffalo (bubalus bubalis) embryonic stem cell. | embryonic stem cells (escs) derived from inner cell mass (icm) of mammalian blastocyst are having indefinite proliferation and differentiation capability for any type of cell lineages. in the present study, icms of in vitro-derived buffalo blastocysts were cultured into two different culture systems using buffalo fetal fibroblast as somatic cell support and matrigel as synthetic support to obtain pluripotent buffalo embryonic stem cell (buesc) colonies. pluripotency of the escs were characterise ... | 2012 | 22678753 |
| the effects of high temperature and roof modification on physiological responses of swamp buffalo (bubalus bubalis) in the tropics. | the objective of the experiments reported here was to measure the effects of cooling techniques (modified roof vs normal roof) on the performance and physiology of 12 young male buffaloes with a similar live weight of 160 kg. the study was conducted at chainat agriculture and technology college, chainat province, thailand. the animals were divided randomly into two groups, each group comprising six buffaloes, and the two groups were studied to evaluate the effects of modified roofing (normal roo ... | 2013 | 22678302 |
| construction and preliminary characterization of a river buffalo bacterial artificial chromosome library. | river buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of bubalus bubalis will be available shortly. nonetheless, large-insert dna library resources such as bacterial artificial chromosomes (bac) are still required for validation and accurate assembly of the genome sequence. we constructed a river buffalo bac library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome. this genomic resource for ri ... | 2012 | 22653673 |
| analysis of in vitro effects of sex steroids on lymphocyte responsiveness in murrah buffaloes (bubalus bubalis). | present study was carried out on forty four apparently healthy murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. estrogen at 100 pg/ml concentration stimulating the proliferation significantly (p < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than ... | 2012 | 22619742 |
| effect of csn1s1-csn3 (α(s1)-κ-casein) composite genotype on milk production traits and milk coagulation properties in mediterranean water buffalo. | the aim of this study was to estimate effects of csn1s1-csn3 (α(s1)-κ-casein) composite genotypes on milk production traits and milk coagulation properties (mcp) in mediterranean water buffalo. genotypes at csn1s1 and csn3 and coagulation properties [rennet clotting time (rct), curd firming time (k₂₀), and curd firmness (a₃₀)] were assessed by reversed-phase hplc and computerized renneting meter analysis, respectively, using single test-day milk samples of 536 animals. alternative protein varian ... | 2012 | 22612978 |
| gonadotropin-releasing hormone 1 directly affects corpora lutea lifespan in mediterranean buffalo (bubalus bubalis) during diestrus: presence and in vitro effects on enzymatic and hormonal activities. | the expression of gonadotropin-releasing hormone (gnrh) receptor (gnrhr) and the direct role of gnrh1 on corpora lutea function were studied in mediterranean buffalo during diestrus. immunohistochemistry evidenced at early, mid, and late luteal stages the presence of gnrhr only in large luteal cells and gnrh1 in both small and large luteal cells. real-time pcr revealed gnrhr and gnrh1 mrna at the three luteal stages, with lowest values in late corpora lutea. in vitro corpora lutea progesterone p ... | 2012 | 22592497 |
| uterine infection influences size and follicular fluid composition of the largest follicle in buffalo (bubalus bubalis). | the effect of uterine infection on size and follicular fluid composition of the largest follicle was studied in buffalo. reproductive tracts were collected from 102 graded murrah buffaloes at an abattoir. uterine infection was diagnosed by physical examination of uterine mucus, white side test and uterine cytology. samples with pus-containing mucus, positive reaction on white side test and/or >5% neutrophils were considered to be positive for uterine infection. diameter of the largest follicle w ... | 2013 | 22583389 |
| equivalency of buffalo (bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic, and hand-made cloned embryos. | this study was aimed at establishing buffalo embryonic stem cells (escs) from in vitro fertilized (ivf), parthenogenetic, and hand-made cloned (hmc) embryos and to check their equivalency in terms of stem cell marker expression, longevity, proliferation, and differentiation pattern. escs derived from all three sources were found by immunofluorescence to express the pluripotency markers ssea-4, tra-1-60, tra-1-81, oct4, and sox2 and were able to form embryoid bodies containing cells expressing ge ... | 2012 | 22582863 |
| power of exclusion of 19 microsatellite markers for parentage testing in river buffalo (bubalus bubalis). | in the present study, 19 microsatellite markers were assessed for their power of exclusion to test parentage in river buffalo. microsatellite genotypes of 216 unrelated buffaloes belonging to five different breeds were utilized for the study. the probabilities of exclusion were calculated for three hypothetical situations viz. paternity testing (pe1), one parental genotype unavailable (pe2) and exclusion of both parents i.e. substituted offspring (pe3). the mean probability of exclusion across 1 ... | 2012 | 22555978 |
| milk progesterone enzyme-linked immunosorbent assay as a tool to investigate ovarian cyclicity of water buffaloes in relation to body condition score and milk production. | application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression. the present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (elisa) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes. | 2012 | 22554129 |
| effect of post-fusion holding time, orientation and position of somatic cell-cytoplasts during electrofusion on the development of handmade cloned embryos in buffalo (bubalus bubalis). | the present study was conducted primarily to optimize electrofusion conditions for efficient production of zona-free nuclear transfer embryos in buffalos (bubalus bubalis). we found that 4v ac current for proper triplet alignment and single step fusion method, using a single dc pulse of 3.36 kv/cm for 4-μs duration, produced the most convincing results for efficient reconstitution of zona-free cloned embryos. lysis rate was very high (84.28 ± 2.59%) when triplets were in physical contact with ne ... | 2012 | 22541327 |
| spermatogonial stem cells (sscs) in buffalo (bubalus bubalis) testis. | water buffalo is an economically important livestock species and about half of its total world population exists in india. development of stem cell technology in buffalo can find application in targeted genetic modification of this species. testis has emerged as a source of pluripotent stem cells in mice and human; however, not much information is available in buffalo. | 2012 | 22536454 |
| expression of transcriptional factor genes (oct-4, nanog, and sox-2) and embryonic stem cell-like characters in placental membrane of buffalo (bubalus bubalis). | the aim of the study was to assess the expression of transcriptional factor genes and embryonic stem cell-like characters in the placental membrane of buffalo (bubalus bubalis). along with the placenta, amniotic fluid, maternal peripheral blood, and umbilical cord blood samples were taken for the future study. the isolation and culture of cells from the placental membrane was followed by the determination of rt-pcr-based markers (oct-4, nanog, sox-2, alkaline phosphatase, stem cell factor, and n ... | 2012 | 22527603 |
| effect of l-arginine methyl ester (l-name) on hormonal profile and estrous cycle length in buffaloes (bubalus bubalis). | the present study was undertaken to evaluate the effect of l-arginine methyl ester (l-name), a nitric oxide synthase inhibitor on serum nitric oxide, progesterone, estradiol profiles and estrous cycle length in buffaloes. murrah buffaloes (n = 16) exhibiting regular estrous cycles were randomly allocated to two groups of eight animals. in the treatment group, buffaloes were administered 400 mg/h l-name over 2 h (total dose = 800 mg) via the coccygeal artery and the aorta abdominalis on day 15 of ... | 2012 | 22476734 |
| expression and characterization of constitutive heat shock protein 70.1 (hspa-1a) gene in in vitro produced and in vivo-derived buffalo (bubalus bubalis) embryos. | cells are blessed with a group of stress protector molecules known as heat shock proteins (hsps), amongst them hsp70, encoded by hspa-1a gene, is most abundant and highly conserved protein. variety of stresses hampers the developmental competence of embryos under in vivo and in vitro conditions. present work was designed to study the quantitative expression of hspa-1a mrna in immature oocytes (imo), matured oocytes (mo), in vitro produced (ivp) and in vivo-derived (ivd) buffalo embryos to assess ... | 2012 | 22463675 |
| embryonic genome activation events in buffalo (bubalus bubalis) preimplantation embryos. | embryonic genome activation (ega) is the first major step towards successful initiation of preimplantation development, which culminates in the formation of implantation-competent embryos. ega occurs at species-specific embryonic cell stages. in the present work, ega was identified for buffalo embryos by studying the development rate of embryos in normal as well as imposed transcription block conditions, analyzing bromo-uridine triphosphate (brutp) incorporation rates as evidence of de novo tran ... | 2012 | 22461405 |
| oxygen concentration and cysteamine supplementation during in vitro production of buffalo (bubalus bubalis) embryos affect mrna expression of bcl-2, bcl-xl, mcl-1, bax and bid. | this study examined the effects of o(2) concentration (5% vs 20%) during in vitro maturation (ivm), fertilization (ivf) and culture (ivc) or supplementation of ivm and ivc media with cysteamine (50 and 100 μm, respectively; ivm, ivf and ivc carried out in 20% o(2)), on blastocyst rate and relative mrna abundance of some apoptosis-related genes measured by real-time qpcr in immature and in vitro-matured buffalo oocytes and in embryos at 2-, 4-, 8- to 16-cell, morula and blastocyst stages. the bla ... | 2012 | 22452597 |
| effect of spermine-nonoate on acrosome reaction and associated protein tyrosine phosphorylation in murrah buffalo (bubalus bubalis) spermatozoa. | the present study was conducted to know the role of nitric oxide (no) on the acrosome reaction (ar) in murrah buffalo (bubalus bubalis) spermatozoa. ejaculated buffalo spermatozoa were washed, suspended in sp-talp media containing 6 mg bsa/ml and cell concentration was adjusted to 50×10(6) cells/ml. the cells were incubated for 6h in the absence or presence of heparin (10 μg/ml) to induce capacitation. fully capacitated spermatozoa were incubated in presence of 100 μg/ml lysophosphatidyl choline ... | 2012 | 22445612 |
| the endocrine changes, the timing of ovulation and the efficacy of the doublesynch protocol in the murrah buffalo (bubalus bubalis). | experiments were conducted to investigate (a) the timing of ovulation and the associated endocrine changes (progesterone, estrogen and lh) during estrous cycle and (b) the efficacy, with respect to the pregnancy rate, in cycling and anestrus in murrah buffaloes subjected to the doublesynch protocol during the low breeding season. in experiment 1, 10 cycling buffaloes were administered pgf(2α) on day 0 (without regard to the estrous cycle stage), gnrh on day 2, a second pgf(2α) injection on day 9 ... | 2012 | 22433942 |
| generation of induced pluripotent stem cells from buffalo (bubalus bubalis) fetal fibroblasts with buffalo defined factors. | ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem cells (ipscs), which initiates a new strategy to obtain pluripotent stem cell lines. attempts have been made to generate buffalo pluripotent stem cells by culturing primary germ cells or inner cell mass, but the efficiency is extremely low. here, we report a successful method to reprogram buffalo fetal fibroblasts (bffs) into pluripotent stem cells [buffalo induced pluripotent stem ce ... | 2012 | 22420535 |
| improved rapd-pcr for discriminating breeds of water buffalo. | | 2012 | 22403004 |
| pcr-sscp analysis of leptin gene and its association with milk production traits in river buffalo (bubalus bubalis). | leptin gene has been found to be associated with various economic traits including milk production and fat quality in dairy animals. in the present study, we investigated genetic variations in intron 1 region of leptin gene in riverine buffaloes (bubalus bubalis) using polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) and sequencing methods and associated them with milk traits. the study revealed three sscp variants a, b and c among a total of 301 buffaloes from nine b ... | 2012 | 22395794 |
| effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating murrah (bubalus bubalis) buffaloes. | the study investigated the effect of dietary supplementation of bypass fat on productive performance and blood biochemical profile of lactating murrah buffaloes (bubalus bubalis). fifteen multiparous buffaloes (2-4 lactation) of early to mid lactation were divided in three homogenous groups t(1) (control), t(2), and t(3) of five each. the animals in t(1) were fed with a basal diet consisting of a concentrate mixture, green sorghum, and wheat straw as per requirements, while the animals in group ... | 2012 | 22373930 |
| roscovitine treatment improves synchronization of donor cell cycle in g0/g1 stage and in vitro development of handmade cloned buffalo (bubalus bubalis) embryos. | this study investigated the effects of serum-starvation, total confluence, and roscovitine treatment on cell-cycle synchronization of buffalo ear skin fibroblasts to the g0/g1 stage and on the developmental competence of cloned embryos. serum starvation of total confluence cultures for 24 h had a higher (p<0.05) proportion of cells at g0/g1 stage (94.4%) compared with serum starved cyclic and nonstarved confluent cultures (76.8 and 86.0%, respectively), whereas differences between cyclic cells w ... | 2012 | 22372577 |
| evaluation of milk trace elements, lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase activity of subclinical mastitis as and indicator of subclinical mastitis in riverine buffalo (bubalus bubalis). | mastitis is a highly morbid disease that requires detection at the subclinical stage. tropical countries like india mainly depend on milch buffaloes for milk. the present study was conducted to investigate whether the trace minerals viz. copper (cu), iron (fe), zinc (zn), cobalt (co) and manganese (mn) and enzyme activity of lactate dehydrogenase (ldh), alkaline phosphatase (alp) and aspartate aminotransferase (ast) in riverine buffalo milk can be used as an indicator of subclinical mastitis (sc ... | 2012 | 25049573 |
| the past and present of and potential for the domestic (water) buffalo in africa. | egypt is the only country in africa where domestic buffalo have a long-term presence and constitute an important part of the array of domestic animal resources. attempts to introduce buffalo to other african countries have been made since at least the 1920s. nine such attempted introductions are documented in this paper, although for most cases, there is very limited information. buffalo have disappeared without trace in some countries and were slaughtered in at least two because of lack of adap ... | 2012 | 22327409 |
| pregnancy-specific protein b (pspb), progesterone and some biochemical attributes concentrations in the fetal fluids and serum and its relationship with fetal and placental characteristics of iraqi riverine buffalo (bubalus bubalis). | this study was carried out to demonstrate the pregnancy-specific protein b (pspb), progesterone and some biochemical parameters concentrations in amniotic fluid, allantoic fluid and fetal serum collected from slaughtered iraqi riverine pregnant buffaloes at three different months of gestation (6th, 7th and 8th). ten out of 22 adult buffaloes of 4.6 ± 0.97 years old were used in this study. the buffaloes were mated naturally by monitoring the estrus cycles via appearance of vaginal fluids and mou ... | 2012 | 22325967 |
| analysis of rumen methanogen diversity in water buffaloes (bubalus bubalis) under three different diets. | the water buffalo (bubalus bubalis) is a prominent livestock species for the production of milk and meat in many countries. we investigated the diversity of rumen methanogens in mediterranean water buffaloes maintained in brazil under different diets: corn silage, grazing pasture, or sugar cane. a total of 467 clones were isolated from three methanogen 16s rrna gene clone libraries that each represented a distinct feed type. the 467 clones were assigned to 19 species-level operational taxonomic ... | 2012 | 22286379 |
| alterations in follicular fluid estradiol, progesterone and insulin concentrations during ovarian acyclicity in water buffalo (bubalus bubalis). | ovarian acyclicity is one of the most important causes of infertility in water buffalo. recent studies have indicated alterations in the composition of follicular fluid during the condition. the aim of this study was to determine the changes in follicular fluid concentrations of estradiol, progesterone and insulin during ovarian acyclicity in water buffalo. ovaries were collected from 50 acyclic and 95 cyclic (control) buffaloes and follicular fluid was aspirated from small (5.0-6.9 mm), medium ... | 2012 | 22277844 |
| production of nuclear transfer embryos by using somatic cells isolated from milk in buffalo (bubalus bubalis). | somatic cells in milk are a potential source of nuclei for nuclear transfer to produce genetically identical animals; this is especially important in animals that are susceptible to risks of bacterial infection on biopsy collection. in this study, a minimum of 10 milk samples were collected from each of the three buffaloes representing murrah breed. all the samples were processed immediately and cell colonies were obtained. cell colonies from one buffalo (mu-442) survived beyond 10 passages and ... | 2012 | 22229797 |
| lacking expression of paternally-expressed gene confirms the failure of syngamy after intracytoplasmic sperm injection in swamp buffalo (bubalus bubalis). | the objectives were to: (1) examine the efficiency of intracytoplasmic sperm injection (icsi) technique, with or without chemical activation of in vitro matured buffalo oocytes, on sperm head decondensation; and (2) compare the subsequent development of embryos after activation of icsi (icsi (+) activation group) and sham injection (sham (+) activation group) oocytes (embryos obtained by in vitro fertilization of ivm oocytes served as a control group). pronuclear formation rates in icsi (+) acti ... | 2012 | 22225682 |
| structural modeling and analysis of pregnancy-associated glycoprotein-1 of buffalo (bubalus bubalis). | the present study was conducted to design and analyze the structural model of buffalo pregnancy-associated glycoprotein-1 (pag-1) using bioinformatics. structural modeling of the deduced buffalo pag-1 protein was done using phyre, consurf servers and its structure was subsequently constructed using modeller 9.9 and pymol softwares buffalo pag-1 structural conformity was analyzed using prosa, whatif, and 3d-pssm servers. designed buffalo pag-1 protein structure on blast analysis retrieved protein ... | 2012 | 27398235 |
| defense cells profile of cervical mucous during follicular and luteal phases of estrus cycle in river buffalo. | the aim of this study was to evaluate the defense cells changes of cervical mucous during follicular and luteal phases of estrus cycle in river buffalo. reproductive organs of the adult and apparently healthy female buffaloes were collected from the slaughterhouse. by visual investigation of both the ovaries for presence of corpus luteum and growing follicles, the luteal and follicular phase of each buffalo was specified. cervical discharge samples were collected by sterile swabs and then spread ... | 2012 | 25653745 |
| escherichia coli o157:h7/nm prevalence in raw beef, camel, sheep, goat, and water buffalo meat in fars and khuzestan provinces, iran. | enterohemorrhagic escherichia coli (ehec) of the o157:h7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human ehec disease. the aim of the present study was to investigate the prevalence of e. coli o157: h7/nm in raw meat samples from two provinces of iran. during a period from march 2010 to march 2011. two hundred and ninety five raw meat samples were collected from beef (n= 85), camel, (n= 50), sheep (n= 62), goat (n= 60), and water buffalo (n=38). fo ... | 2012 | 25653740 |
| molecular identification of methanogenic archaea from surti buffaloes (bubalus bubalis), reveals more hydrogenotrophic methanogens phylotypes. | methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouses gases contributing to global warming. many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. therefore, a thorough knowledge of the diversity of these microbes in breeds of buffaloes, as well as in response to geographical location and different diets, i ... | 2011 | 24031614 |
| effect of bromocryptine on hormones and milk secretion in murrah buffaloes ( bubalus bubalis). | an experiment was carried out on 10 advance pregnant murrah buffaloes to determine the role of hormones in milk secretion around parturition. experimental animals were administered with a single injection of bromocryptine, @ 100 μg/kg bw, for 5 days before expected calving, whereas control group buffaloes were injected with placebo injections. blood samples collected before parturition (-5,-4,-3,-2,-1 days), on day of parturition (day-0) and on day 1, 2, 3, 4, 5, 10 and 15 post partum were analy ... | 2010 | 22444132 |
| α-tocopherol and l-ascorbic acid increase the in vitro development of ivm/ivf swamp buffalo ( bubalus bubalis) embryos. | this study was conducted to investigate the effects of capacitating agents added at in vitro fertilization (ivf) and antioxidants supplemented during in vitro culture (ivc) on the development of buffalo embryos. in experiment i, in vitro embryo development of buffalo embryos was compared when the ivf medium was supplemented with heparin, caffeine and calcium ionophore a23187 either alone or in combination. there was no significant difference (p > 0.05) in the cleavage rates of oocytes among the ... | 2008 | 22443906 |
| characterization of osteopontin gene of bubalus bubalis. | osteopontin, a glycoprotein, is expressed in several tissues including the mammary gland. the gene has been reported to be associated with milk and its constituents in various livestock species. this gene was sequenced in buffalo and it coded for the protein of 280 amino acids with the conserved grgds domain. the sequence was confirmed from the cdna sequence derived from the mammary gland of buffalo. the earlier-reported 9t/10t variation in the upstream region of the gene was investigated for it ... | 2008 | 22443697 |
| detailed description of rba-banded chromosomes of river buffalo (bubalus bubalis l.). | | 1984 | 22879164 |
| the c-banding pattern of the egyptian water buffalo (bubalus bubalis). | | 1978 | 22896102 |
| ethnomedicinal and cultural practices of mammals and birds in the vicinity of river chenab, punjab-pakistan. | although, use of animal species in disease treatment and culture practices is as ancient as that of plant species; however ethnomedicinal uses and cultural values of animal species have rarely been reported. present study is the first report on the medicinal uses of mammals and bird species in pakistan. | 2017 | 28701165 |
| fixed differences in the 3'utr of buffalo prnp gene provide binding sites for mirnas post-transcriptional regulation. | bovine spongiform encephalopathy, a member of transmissible spongiform encephalopathies, has not been reported in buffaloes, bubalus bubalis. prion protein (prp), encoded by the prion protein gene (prnp), is fundamental in the pathogenesis of transmissible spongiform encephalopathies. we previously showed that buffaloes express more prp proteins but lower prnp mrna than cattle in several pivotal tissues like the obex. therefore, we sought to establish whether genetic variability in prnp 3'utr, m ... | 2017 | 28545018 |
| serotyping, antibiotic susceptibility, and virulence genes screening of escherichia coli isolates obtained from diarrheic buffalo calves in egyptian farms. | in egypt as in many other countries, river water buffalo (bubalus bubalis) is considered an important source of high-quality milk and meat supply. the objective of this study was to investigate serotypes, virulence genes, and antibiotic resistance determinants profiles of escherichia coli isolated from buffalo at some places in egypt; noticibly, this issue was not discussed in the country yet. | 2017 | 28831220 |
| serological survey of neospora caninum and toxoplasma gondii in cattle (bos indicus) and water buffaloes (bubalus bubalis) in ten provinces of brazil. | the objective of this study was to determine the prevalence of antibodies to neospora caninum and toxoplasma gondii among 500 cattle (bos indicus) and 500 buffaloes (bubalus bubalis) using the indirect fluorescent antibody test (ifat) technique. blood samples from were collected from water buffalo and cattle in 10 municipalities in the northern region of brazil. the frequency of cattle and water buffaloes seropositive for neospora caninum in pará state, brazil, was 55% and 44%, respectively, and ... | 2017 | 28673459 |
| surveillance for hemorrhagic septicemia in buffalo (bubalus bubalis) as an aid to range expansion of the javan rhinoceros (rhinoceros sondaicus) in ujung kulon national park, indonesia. | the javan rhinoceros (rhinoceros sondaicus) of ujung kulon national park (uknp) is the crown jewel of indonesia's rich natural history. the park lies on a peninsula surrounded by coastline and agriculture-dominated landscapes. the invasion of water buffalo (bubalus bubalis) into the park carries a substantial health risk to the javan rhinoceros and threatens plans to establish a new population outside of its only current range in uknp. hemorrhagic septicemia (hs), known locally as septicemia epi ... | 2017 | 28820298 |
| sarcocystis dehongensis n. sp. (apicomplexa: sarcocystidae) from water buffalo (bubalus bubalis) in china. | water buffalo (bubalus bubalis) is the intermediate host for at least four species of sarcocystis: s. fusiformis, s. buffalonis, s. levinei, and s. sinensis/s. dubeyi. here, a new species, sarcocystis dehongensis, is reported in 51 of 756 (6.7%) water buffaloes in china. by light microscopy, the cysts of s. dehongensis were macroscopic, up to 18.5 mm long and 95 μm in diameter; 4.9-11.9 μm villous protrusions extended beyond the sarcocyst wall. using transmission electron microscopy, the sarcocy ... | 2017 | 28560573 |
| detomidine and butorphanol for standing sedation in a range of zoo-kept ungulate species. | general anesthesia poses risks for larger zoo species, like cardiorespiratory depression, myopathy, and hyperthermia. in ruminants, ruminal bloat and regurgitation of rumen contents with potential aspiration pneumonia are added risks. thus, the use of sedation to perform minor procedures is justified in zoo animals. a combination of detomidine and butorphanol has been routinely used in domestic animals. this drug combination, administered by remote intramuscular injection, can also be applied fo ... | 2017 | 28920785 |
| a fast and reliable real-time pcr method for detection of ten animal species in meat products. | species substitution in meat products is a common problem reported worldwide. this type of food fraud is, typically, an intentional act for economic gain, using sources of low-priced meats in high-value meat products. consequences include economic, health, and religious concerns. highly sensitive and efficient techniques are thus required to detect meat species. this paper describes a method based on real-time pcr to detect 10 animal species (bos taurus, sus scrofa, ovis aries, capra hircus, gal ... | 2018 | 29377112 |
| spatial-temporal and phylogeographic characterization of trypanosoma spp. in cattle (bos taurus) and buffaloes (bubalus bubalis) reveals transmission dynamics of these parasites in colombia. | animal trypanosomiasis (at) is one of the most important problems in the colombian livestock industry reducing its production around 30%. caribbean and orinoquia regions play a significant role in the development of this industry, having about 6.9 million cattle and 113,000 buffaloes. considering the paucity in studies to understand the epidemiological features and control of at in colombia, the present study reports the seasonal transmission patterns and phylogeographic traits of the causal age ... | 2018 | 29279084 |
| species-specific and differential expression of bsp-5 and other bsp variants in normozoospermic and asthenozoospermic buffalo (bubalus bubalis) and cattle (bos taurus) seminal plasma. | binder of sperm-5 (bsp-5) is one of the fertility-associated proteins of cattle seminal plasma. binding of sperm to the oviductal epithelium is mediated by bsp group of proteins. however, it is not clear, whether this protein is also involved in sperm motility. in the present study, attempts were made to characterize bsp-5 protein in both normozoospermic (ns) and asthenozoospermic (as) murrah buffalo (n = 18; bubalus bubalis), holstein friesian (n = 8, bos taurus) and jersey cattle (n = 8; bos t ... | 2018 | 29100133 |
| characteristics of yak platelet derived growth factors-alpha gene and expression in brain tissues. | platelet derived growth factors (pdgfs) are key components of autocrine and paracrine signaling, both of which play important roles in mammalian developmental processes. pdgf expression levels also relate to oxygen levels. the characteristics of yak pdgfs, which are indigenous to hypoxic environments, have not been clearly described until the current study. | 2017 | 28553854 |
| partial purification and characterization of glutathione s-transferase from the somatic tissue of gastrothylax crumenifer (trematoda: digenea). | aim of the present study was to carry out the partial purification and biochemical characterization of glutathione s-transferase (gst) from the somatic tissue of ruminal amphistome parasite, gastrothylax crumenifer (gc) infecting indian water buffalo (bubalus bubalis). | 2017 | 29391692 |
| identification of micrornas in corpus luteum of pregnancy in buffalo (bubalus bubalis) by deep sequencing. | this study was aimed to identify mirnas of corpus luteum (cl) in buffaloes during pregnancy. for this study, cl (n=2) were collected from gravid uteri of buffalo and rna was isolated. following this, the purity and integrity of rna was checked and used for deep sequencing using illumina hiseq 2500 platform. the reads' quality was checked prior to in silico analyses viz. identification of conserved, novel and target of mirnas. in this study, out of identified mirnas (3018), 3013 were known and 5 ... | 2017 | 29387104 |
| global proteomic analysis of water buffalo (bubalus bubalis) saliva at different stages of estrous cycle using high throughput mass spectrometry. | accurate and efficient detection of estrus is one of the major constraints for exploitation of the production potential of buffalo owing to its poor manifestation of estrus signs, seasonal differences in expression and higher incidences of silent estrus (29%). the current study focused on identification of estrus specific candidate proteins in saliva of buffaloes. estrus was detected based on behavioral signs in response to the teaser and changes in reproductive organs and confirmed by per-recta ... | 2018 | 29334660 |
| role of bcl2l10 in regulating buffalo (bubalus bubalis) oocyte maturation. | it has been reported that bcl2l10 is abundantly and specifically expressed in adult human and mouse oocytes and played a very important role in oocytes maturation and early embryonic development. this study is to investigate the expression pattern of bcl2l10 in buffalo ovaries and its effect on the in vitro maturation of buffalo oocytes, so as to dissect mechanism of oocytes maturation and provide theoretical guidance for improvement of the in vitro maturation of buffalo oocytes. the results sho ... | 2017 | 29331495 |
| correction: impact of heat stress on cellular and transcriptional adaptation of mammary epithelial cells in riverine buffalo (bubalus bubalis). | [this corrects the article doi: 10.1371/journal.pone.0157237.]. | 2018 | 29324889 |
| significance of clinical observations and biochemical alterations in buffalo calves with dietary abomasal impaction. | the present study aimed to throw light on the clinical characteristics of abomasal impaction in buffalo calves and its associated biochemical alterations. for this reason, a total of 20 male buffalo calves (bubalus bubalis) with abomasal impaction were studied. the investigated calves were at 6 to 12 months of age and were belonged to three private farms in dakahlia governorate besides sporadic cases admitted to the veterinary teaching hospital, faculty of veterinary medicine, mansoura universit ... | 2018 | 29291740 |
| incubation of spermatozoa with anandamide prior to cryopreservation reduces cryocapacitation and improves post-thaw sperm quality in the water buffalo (bubalus bubalis). | anandamide (aea), an endocannabinoid, has been shown to reduce capacitation and acrosomal exocytosis in human spermatozoa. because buffalo spermatozoa are highly susceptible to cryopreservation induced damage, aea was assessed as to whether it could protect spermatozoa from cryo-damage. six ejaculates from six murrah buffalo bulls (total 36 ejaculates) were utilized for the study. each ejaculate was divided into four aliquots; spermatozoa in aliquot 1 were extended in tris-citrate-egg yolk and f ... | 2018 | 29277315 |
| pregnancy rate in water buffalo following fixed-time artificial insemination using new or used intravaginal devices with two progesterone concentrations. | this study evaluated the pregnancy rate (pr) after timed artificial insemination (tai) in water buffalo (bubalus bubalis) during both non-breeding and breeding season, using either a new or reused intravaginal device (ivd) with two different progesterone concentrations. a total of 247 dairy buffalo cows were randomly assigned using a two-by-three factorial design and four replicates to the following groups: (1) new intravaginal device (ivd-new: dib®, 1.0 g of p4, n = 51 or cidr®, 1.38 g of p4, n ... | 2017 | 29274053 |
| virus neutralization technique as a tool to evaluate the virological profile for bovine viral diarrhea virus infection in dairy water buffalo (bubalus bubalis) herds. | serological evidence shows that the bovine viral diarrhea virus (bvdv) infections are present in brazilian dairy and beef water buffalo herds. as few reports describe the bvdv infection profile the aim of this study was to evaluate the dynamics of bvdv circulation in brazilian dairy water buffalo herds through analysis of the seropositivity rate and the titer range of anti-bvdv neutralizing antibodies in a group of animals that are considered sentinels. blood samples (n = 305) were obtained from ... | 2017 | 29260489 |
| partial deoxygenation of extender improves sperm quality, reduces lipid peroxidation and reactive oxygen species during cryopreservation of buffalo (bubalus bubalis) semen. | the present study was designed to investigate the effect of partial deoxygenation of extender on sperm quality, lipid peroxidation (lpo) and reactive oxygen species (ros) in buffalo (bubalus bubalis) during cryopreservation of semen. semen extender was prepared freshly and split into three sub-extenders [extender i: control (non-deoxygenated), extender ii (partially deoxygenated by using ln2 flushing) and extender iii (partially deoxygenated mechanically by vacuum pump)]. amounts of dissolved ox ... | 2018 | 29258709 |
| short communication: stall occupancy behavior of nili ravi buffaloes (bubalus bubalis) when first introduced to freestall housing. | the aim of the present study was to assess stall occupancy of nili ravi buffaloes when first introduced to freestall housing. thirty nili ravi buffaloes of different parity, weight, and pregnancy status were enrolled from the dairy farm of the university of veterinary and animal sciences, lahore, pakistan. all of the enrolled animals had no previous experience with freestalls. the selected buffaloes were introduced to the freestall shed. during an adaptation period of 7 d, all buffaloes had 24 h ... | 2018 | 29224871 |
| the functional and molecular studies on involvement of hydrogen sulphide in myometrial activity of non-pregnant buffaloes (bubalus bubalis). | hydrogen sulphide (h2s), a member of the gasotransmitters family, is known to play patho-physiological role in different body systems including during pregnancy. but its involvement in myometrial spontaneity and associated signalling pathways in uterus in non-pregnant animals is yet to be studied. present study describes the effect of l-cysteine, an endogenous h2s donor, on isolated myometrial strips of non-pregnant buffaloes and the underlying signaling mechanism(s). | 2017 | 29207994 |
| an association analysis between prl genotype and milk production traits in italian mediterranean river buffalo. | this research communication describes the association between genetic variation within the prolactin (prl) gene and the milk production traits of italian mediterranean river buffalo (bufala mediterranea italiana). high resolution melting (hrm) techniques were developed for genotyping 465 buffaloes. the association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. single nucleotide polymorphisms (snps) were ... | 2017 | 29154740 |
| fasciola hepatica infection in water buffalo bubalus bubalis in three provinces of the nile delta, egypt: a cross-sectional study. | a cross-sectional study was conducted to estimate the prevalence of fasciola hepatica (f. hepatica) infection in water buffalo (bubalus bubalis) in alexandria, beheira, and kafr el-sheikh governorates (provinces) of the nile delta in egypt and to identify the underlying risk factors associated with the infection. a total of 29 farms (10 in alexandria, 10 in beheira, and 9 in kafr el-sheikh) were randomly selected and all the buffaloes that resided on these farms from 21 february 2015 to 20 febru ... | 2018 | 29151443 |
| effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (bubalus bubalis). | the present study was undertaken to investigate the effects of preovulatory follicle (pof) size on estradiol concentrations, luteal profile (cl diameter and progesterone concentration) and subsequent pregnancy rate in murrah buffalo cows. the buffalo cows (n = 49) were synchronized for estrus by two doses of pgf2α given 11 days apart. the buffalo cows were inseminated during standing estrus and again after 24 h. ovaries were scanned at estrus and 24 h intervals until ovulation, thereafter on day ... | 2018 | 29128702 |
| clinical efficacy and pharmacokinetics of meloxicam in mediterranean buffalo calves (bubalus bubalis). | the aims of the investigation were to establish for the first time (i) clinical efficacy and (ii) pharmacokinetic profile of meloxicam intravenously (iv) administered in male mediterranean buffalo calves after surgical orchiectomy. the study was performed on 10 healthy buffalo calves, between 4 and 5 months old and between 127 and 135 kg of body weight (b.w.). an iv injection of 0.5 mg/kg b.w. of meloxicam was administered in six calves (treated group, tg) immediately after surgery; the other fo ... | 2017 | 29077759 |
| repertoire of noncoding rnas in corpus luteum of early pregnancy in buffalo (bubalus bubalis). | the present study was designed to identify other noncoding rnas (ncrnas) in the corpus luteum (cl) during early pregnancy in buffalo. | 2017 | 29062204 |
| genome assembly and transcriptome resource for river buffalo, bubalus bubalis (2n = 50). | water buffalo is a globally important species for agriculture and local economies. a de novo assembled, well-annotated reference sequence for the water buffalo is an important prerequisite for studying the biology of this species, and is necessary to manage genetic diversity and to use modern breeding and genomic selection techniques. however, no such genome assembly has been previously reported. there are 2 species of domestic water buffalo, the river (2 n = 50) and the swamp (2 n = 48) buffalo ... | 2017 | 29048578 |
| buffalo heifers selected for lower residual feed intake have lower feed intake, better dietary nitrogen utilisation and reduced enteric methane production. | this study was conducted to evaluate the utilisation of the residual feed intake (rfi) as a feed efficiency selection tool and its relationship with methane emissions. eighteen murrah buffalo (bubalus bubalis) heifers were fed ad libitum with total mixed ration (tmr) for 120 days. based on linear regression models involving dry matter intake (dmi), average daily gain (adg) and mid-test metabolic body size (mbw0.75 ), heifers were assigned into low and high rfi groups. the rfi varied from -0.09 t ... | 2017 | 29027698 |
| design and validation of a 90k snp genotyping assay for the water buffalo (bubalus bubalis). | the availability of the bovine genome sequence and snp panels has improved various genomic analyses, from exploring genetic diversity to aiding genetic selection. however, few of the snp on the bovine chips are polymorphic in buffalo, therefore a panel of single nucleotide dna markers exclusive for buffalo was necessary for molecular genetic analyses and to develop genomic selection approaches for water buffalo. the creation of a 90k snp panel for river buffalo and testing in a genome wide assoc ... | 2017 | 28981529 |
| trends in the quality and hygiene parameters of bulk italian mediterranean buffalo (bubalus bubalis) milk: a three year study. | buffalo milk represents an indispensable source of nourishment in many parts of the world and it is the second most consumed milk worldwide. buffalo milk is actually used for the production of many dairy products such as pasteurized or concentrated milk, butter, yogurt, ice-cream, dehydrated milk products and cheeses. due to its high nutritional value and the presence of natural bioactive substances, buffalo milk can also provide health benefits to consumers. in italy, buffalo milk is used mainl ... | 2018 | 28961355 |
| assessment of a specifically developed bullet casing gun for the stunning of water buffaloes. | water buffaloes and cattle differ considerably with respect to the anatomy of the head. as a result, captive bolt stunners often fail to reliably produce adequate loss of consciousness in water buffaloes and, thus, do not fulfill animal welfare requirements. the goal of the present study was to assess and validate a new stunning device for water buffaloes meeting animal welfare and occupational safety requirements. the newly designed bullet casing gun uses .357mag/10.2g hollow point bullets and ... | 2018 | 28938106 |
| genetic perspective of milk yield persistency in the first three lactations of iranian buffaloes (bubalus bubalis). | the objectives of this study were to estimate the genetic parameters and genetic trends for different measures of persistency (pm) for milk yield in iranian buffaloes using a random regression test day model. test day records of the first three lactations of buffaloes within 715 herds comprising 43 014, 38 941 and 33 937 records, respectively, were obtained from the animal breeding center of iran during 1992 to 2012. legendre polynomial functions were chosen to fit the lactation curves in the fr ... | 2017 | 28929983 |
| withdrawn: differential involvement of l- and t-type ca2+ channels, store-operated calcium channel (trpc) and rho-kinase signaling pathway(s) in pgf2α-induced contractions in myometrium of non-pregnant and pregnant buffaloes (bubalus bubalis). | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. | 2017 | 28916261 |
| selection of suitable internal control genes for accurate normalization of real-time quantitative pcr data of buffalo (bubalus bubalis) blastocysts produced by scnt and ivf. | we evaluated the suitability of 10 candidate internal control genes (icgs), belonging to different functional classes, namely actb, eef1a1, gapdh, hprt1, hmbs, rps15, rps18, rps23, sdha, and ubc for normalizing the real-time quantitative polymerase chain reaction (qpcr) data of blastocyst-stage buffalo embryos produced by hand-made cloning and in vitro fertilization (ivf). total rna was isolated from three pools, each of cloned and ivf blastocysts (n = 50/pool) for cdna synthesis. two different ... | 2017 | 28880574 |
| anandamide exerts a suppressive effect on sperm binding to oviduct explants through cb1 receptors in the water buffalo (bubalus bubalis). | an endocannabinoid system comprising of anandamide (aea) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. in the present study, we assessed the effect of sperm pre-treatment with aea or co-incubation of sperm-oviduct explants with aea in the presence or absence of cb1 receptor antagonist (sr141716a) on sperm-oviduct binding in the water buffalo. cryopreserved spermatozoa from 3 murrah buffalo bulls (3 ejaculates from each ... | 2017 | 28869110 |
| the first serological evidence for rift valley fever infection in the camel, goitered gazelle and anatolian water buffaloes in turkey. | rift valley fever (rvf), a vector-borne zoonotic disease, is caused by a phlebovirus (family bunyaviridae). the virus was initially characterized approximately 80 years ago in kenya and disseminated to many countries in the continental africa, saudi arabia, and yemen. the infection has not been reported in turkey. in this study, blood serum samples collected from camel (camelus dromedairus), goitered gazelle (gazella subgutturosa subgutturosa), and buffaloes (bubalus bubalis linneaus) from 2000 ... | 2017 | 28861841 |
| biocomputational identification and validation of novel micrornas predicted from bubaline whole genome shotgun sequences. | micrornas (mirnas) are small (19-25 base long), non-coding rnas that regulate post-transcriptional gene expression by cleaving targeted mrnas in several eukaryotes. the mirnas play vital roles in multiple biological and metabolic processes, including developmental timing, signal transduction, cell maintenance and differentiation, diseases and cancers. experimental identification of micrornas is expensive and lab-intensive. alternatively, computational approaches for predicting putative mirnas fr ... | 2017 | 28844020 |
| morphological studies of the tongue of the egyptian water buffalo (bubalus bubalis) and their lingual papillae adaptation for its feeding habits. | this work was conducted to describe the morphological characters of the tongue of egyptian water buffalo (bubalus bubalis). the lingual root and the dorsal middle region of apex and body in addition to the dorsal and ventral surface of lingual tip were devoided from any fungiform papillae. the lingual tip contains conical papillae only. the ventral surface of lingual apex was divided into two portions by the u-shaped fungiform line into papillary and non-papillary region. histological investigat ... | 2017 | 28833390 |
| analysis of microsatellite markers in a cuban water buffalo breed. | the aim of this regional research communication was to validate a panel of 30 microsatellite markers recommended by fao/isag for studies of biodiversity in cattle to improve the characterisation of cuban buffalo populations. the water buffalo (bubalus bubalis) is an economically important livestock species. therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. twenty-eight ... | 2017 | 28831973 |
| comparative therapeutic effect of steroidal and non-steroidal anti-inflammatory drugs on pro-inflammatory cytokine production in water buffalo calves (bubalus bubalis) naturally infected with bronchopneumonia: a randomized clinical trial. | in the current study, we compared the therapeutic effects of a non-steroidal and a steroidal anti-inflammatory drug on the production of pro-inflammatory cytokines, interleukin-1β (il-1β), interleukin-6 (il-6), interleukin-12p40 (il-12p40), interferon gamma (ifnγ), and tumor necrosis factor alpha (tnf-α) in the blood of water buffalo (bubalus bubalis) calves naturally infected by bronchopneumonia. twenty-seven buffalo calves (7 ± 2-month-old, 163 ± 12 kg) reared in smallholder farms in el-dakahl ... | 2017 | 28831754 |
| determination of an optimal membrane-permeable cryoprotectant addition and dilution protocol for water buffalo spermatozoa. | there is a possibility to reduce the toxicity of glycerol and osmotic stress of dmso by lowering their concentrations in freezing extenders. | 2017 | 28767747 |
| biochemical analysis of uterine fluid for identification of indicators for subclinical endometritis in the water buffalo (bubalus bubalis). | alterations in biochemical constituents of uterine fluid have been suggested for diagnosis of subclinical uterine infection in the bovine. this study was undertaken to investigate whether uterine fluid biomolecules could act as tool for diagnosis of subclinical endometritis in the buffalo. uterine fluid samples from normal (n = 22) and subclinical endometritis (n = 18; diagnosed based on uterine cytology)-affected buffaloes were subjected to biochemical analysis. among the different biochemical ... | 2018 | 28804962 |
| evaluation of α-linolenic acid for freezability and in vivo fertility of nili ravi (bubalus bubalis) buffalo semen. | alpha linolenic acid (ala) is integral component of cell membrane that protects the cell in stressful events and involves in many metabolic pathways. it was hypothesized that ala have the ability to protect the structural and functional integrity of buffalo spermatozoa during freeze-thawing. therefore, study was designed to evaluate ala supplementation (0, 5, 10 and 20 ng/ml) in extender on freezability and in vivo fertility of buffalo bull spermatozoa. semen from three adult nili-ravi buffalo b ... | 2017 | 28802112 |
| molecular characterization and comparison of phospholipase c zeta (plcz1) gene between swamp (bubalus carabanensis) and riverine (bubalus bubalis) buffaloes: its implications and future perspectives. | phospholipase c zeta, a novel sperm-specific protein which is widely known to induce oocyte activation following fertilization, had already been characterized in various mammalian species, but not in water buffaloes thus far. the present study was conducted to initially characterize and compare the sequences of plcz1 gene of swamp and riverine buffaloes. semen samples were collected; total rna was extracted and reverse-transcribed. plcz1 cdna was then amplified, and submitted for sequencing. buf ... | 2017 | 28799828 |
| influence of season, age and management on scrotal thermal profile in murrah bulls using scrotal infrared digital thermography. | the aim of the present study was to examine the effects of non-genetic factors on scrotal thermographic profile viz., proximal pole temperature (ppt °c), mid pole temperature (mpt °c), distal pole temperature (dpt °c) and ocular temperature (oct) of murrah (bubalus bubalis) breeding bulls. a total of 109 buffalo bulls, maintained at three semen stations (ss), were monitored for scrotal surface and ocular temperatures using infrared thermography twice daily during rainy, winter and summer seasons ... | 2017 | 28791483 |
| effect of supplementation of recombinant regucalcin in extender on cryopreservation of spermatozoa of water buffalo (bubalus bubalis). | elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. regucalcin (rgn), also known as senescence marker protein-30 (smp-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. in drosophila, rgn is reportedly a putative cold-tolerance gene and a cytoprotective role for rgn against intracellular calcium elevation ... | 2017 | 28782859 |
| transcript analysis at dgat1 reveals different mrna profiles in river buffaloes with extreme phenotypes for milk fat. | buffalo dgat1 (diacylglycerol o-acyltransferase 1) was mainly investigated for the characterization of the gene itself and for the identification of the k232a polymorphism, similar to what has been accomplished in cattle, although no information has been reported so far at the mrna level. the importance of dgat1 for lipid metabolism led us to investigate the transcript profiles of lactating buffaloes characterized as high (9.13 ± 0.23) and low (7.94 ± 0.29) for milk fat percentage, and to explor ... | 2017 | 28780112 |
| changes in some blood parameters, milk composition and yield of buffaloes (bubalus bubalis) during the transition period. | in this study changes in hematochemical parameters, milk composition and yield were investigated in buffaloes during the transition period. a total of 93 buffaloes 113.9 ± 8.03 months old and 535 ± 50 kg average body weight were used. parity was recorded, blood samples were collected from 80 days pre-partum until 70 days post-partum; milk samples were collected from 5 days to 70 days post-partum. on serum samples, the values of non-esterified fatty acids, β-hydroxybutyrate, glucose, cholesterol, ... | 2017 | 28776872 |
| in-gel and offgel-based proteomic approach for authentication of meat species from minced meat and meat products. | fraudulent mislabelling of processed meat products on a global scale that cannot be detected using conventional techniques necessitates sensitive, robust and accurate methods of meat authentication to ensure food safety and public health. in the present study, we developed an in-gel (two-dimensional gel electrophoresis, 2de) and offgel-based proteomic method for authenticating raw and cooked water buffalo (bubalus bubalis), sheep (ovis aries) and goat (caprus hircus) meat and their mixes. | 2018 | 28737240 |
| retinoic acid induces differentiation of buffalo (bubalus bubalis) embryonic stem cells into germ cells. | development of precise and reproducible culture system for in vitro differentiation of embryonic stem (es) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (pgcs), but in finally treating the catastrophic infertility. taking into account the significant role of retinoic acid (ra) during in vivo gametogenesis, we designed the present study to invest ... | 2017 | 28736154 |
| genomic differentiation between swamp and river buffalo using a cattle high-density single nucleotide polymorphisms panel. | buffalo (bubalus bubalis) is an important livestock species in many tropical and subtropical regions. in recent decades, the interest in buffalo's milk have expanded and intensive buffalo farms start to emerge. however, breeding programs and population genetics information for this species is scarce or inexistent. the present study aims to test the suitability of the commercial high-density single nucleotide polymorphisms (snp) genotyping panel, the illumina bovinehd beadchip, to estimate popula ... | 2017 | 28735584 |
| influence of body condition score at calving on the metabolic status and production performance of murrah buffaloes (bubalus bubalis) during the transition period. | the purpose of this study was to evaluate the influence of body condition score (bcs) at calving on the metabolic status of female murrah buffaloes in the transition period. | 2017 | 28728402 |
| ultrastructure changes in buffalo (bubalus bubalis) oocytes before and after maturation in vitro with sericin. | the aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (bubalus bubalis). oocytes were matured in vitro in tissue culture medium-199 with and without sericin, and then analyzed by light and transmission electron microscopy. the experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (p < 0.05). the immature oocyte ... | 2017 | 28722264 |
| expression and molecular cloning of interferon stimulated genes in buffalo (bubalus bubalis). | buffalo, the most important livestock species in tropical india, remains to be a poor breeder mainly due to embryonic mortality (65%) occurring mostly between 16 and 18 days of pregnancy. early and accurate diagnosis of pregnancy can thus become a boon for successful herd management in buffalo. however, most of the currently available methods allow diagnosis only after 30 days post ai. interferon tau (ifnt), the first pregnancy recognition signal in ruminants is one such molecule, which stimulat ... | 2017 | 28708533 |
| whole mitogenomes reveal the history of swamp buffalo: initially shaped by glacial periods and eventually modelled by domestication. | the newly sequenced mitochondrial genomes of 107 asian swamp buffalo (bubalus bubalis carabensis) allowed the reconstruction of the matrilineal divergence since ~900 kya. phylogenetic trees and bayesian skyline plots suggest a role of the glacial periods in the demographic history of swamp buffalo. the ancestral swamp-buffalo mitogenome is dated ~232 ± 35 kya. two major macro-lineages diverged during the 2nd pleistocene glacial period (~200-130 kya), but most (~99%) of the current matrilines der ... | 2017 | 28680070 |
| comparative efficacy of oestrus synchronization protocols in buffalo (bubalus bubalis). | this study was conducted to test the efficacy of gonadotropic hormone (gnrh)-based synchronization protocols (ovsynch, heatsynch, and ovsynch plus) in buffaloes under field condition. based on anamnesis and transrectal palpation twice at 10-day interval and serum progesterone (p4) concentration, 150 anoestrous buffaloes and delayed pubertal heifers were selected to induce oestrus using gnrh-based protocols. these selected animals were randomly divided into three groups: group i: ovsynch (n = 50) ... | 2017 | 28669062 |