| tyrosine phosphorylation of the tetragonal paracrystalline array of aeromonas hydrophila: molecular cloning and high-level expression of the s-layer protein gene. | high virulence strains of the fish pathogenic bacterium aeromonas hydrophila produce a tetragonally arranged paracrystalline surface protein array (s-layer). the gene (ahsa) encoding the s-protein subunit of a. hydrophila tf7 was cloned into lambda embl 3, and sub-cloned into puc 18. transformation into escherichia coli led to stable high-level expression of full-size s-protein under the control of its native promoter. the dna sequence revealed a 1406 base-pair open reading frame encoding a prot ... | 1995 | 7844827 |
| presence of aeromonas hydrophila in slaughtered animals. | the fate of aeromonas hydrophila after a bacteriaemia in four healthy pigs intravenously inoculated with 10(9) and 10(15) cells of the bacterium was investigated. in two pigs slaughtered 30 min after inoculation the bacteria were found in blood (8 x 10(2)/ml and 4.2 x 10(2)/ml), meat and fatty tissues (1-10/g), but not in liver, spleen and superficial inguinal lymph nodes. a. hydrophila was not found in blood, meat, fatty tissue, liver, spleen, kidneys and superficial inguinal lymph nodes of oth ... | 1994 | 7848784 |
| comparative in vitro activity of fk-037, a new cephalosporin antibiotic. | the in vitro activity of fk-037, a novel parenteral oxime-type cephalosporin, was compared with that of cefepime, cefpirome, ceftazidime, imipenem, and gentamicin against a total of 668 recent clinical isolates. minimum inhibitory concentrations were determined by a standard agar dilution procedure, and all isolates were tested at two inocula (10(4) and 10(6) colony forming units). fk-037 inhibited 90% of isolates of escherichia coli, klebsiella species, proteus mirabilis, p. vulgaris, morganell ... | 1994 | 7867295 |
| variability of peptidoglycan structural parameters in gram-negative bacteria. | muropeptide composition of peptidoglycan from the gram-negative bacteria aeromonas sp., acinetobacter acetoaceticus, agrobacterium tumefaciens, enterobacter cloacae, proteus morganii, pseudomonas aeruginosa, pseudomonas putida, vibrio parahaemolyticus yersinia enterocolitica and escherichia coli, was analyzed by hplc. in all instances peptidoglycan was built up from the same subunits. a wide disparity in the relative abundance of muropeptides and all structural parameters was observed. the contr ... | 1995 | 7867925 |
| the periplasmic endonuclease i of escherichia coli has amino-acid sequence homology to the extracellular dnases of vibrio cholerae and aeromonas hydrophila. | the gene enda, encoding the periplasmic endonuclease i (endoi) of escherichia coli, was identified on a cloned chromosomal 1.5-kb hindiii fragment. the nucleotide sequence of the fragment revealed an open reading frame (orf) coding for a polypeptide of 235 amino acids (aa). the orf preceeded by a region with two possible promoter sites displays promoter activity when cloned into an expression vector. on the c-terminal side, two sequences with putative transcription termination function are prese ... | 1995 | 7867949 |
| cloning, sequence and regulation of expression of the lexa gene of aeromonas hydrophila. | the lexa gene of aeromonas hydrophila (ah) has been isolated by using a specific one-step cloning system. the ah lexa repressor is able to block escherichia coli (ec) sos gene expression and is likely to be cleaved by the activated reca protein of this bacterial species after dna damage. ah lexa would encode a protein of 207 amino acids (aa), which is 75% identical to the lexa repressor of ec. two ec-like sos boxes have been located upstream from ah lexa, the distance between them being 4 bp, wh ... | 1995 | 7867953 |
| septicaemia caused by aeromonas hydrophilia in a neonate. | | 1994 | 7868498 |
| enterotoxigenicity, hemolytic activity and antibiotic resistance of aeromonas spp. isolated from freshwater prawn marketed in dhaka, bangladesh. | aeromonas spp. were isolated from gills, swimmerets, eggs, stomachs and ventral muscles of freshwater prawns (macrobrachium malcolmsonii) available in the local fish market of dhaka, bangladesh. the density of aeromonas spp. on these different body parts of the prawn samples ranged from 1.1 +/- 0.2 x 10(4) to 1.5 +/- 0.16 x 10(7) cfu per gram. the viable counts of aeromonads, fecal coliforms (fc) and escherichia coli gradually increased in prawn samples when stored at 4 c. at -20 c, the viable c ... | 1994 | 7869955 |
| detection of shigellae, enteroinvasive and enterotoxigenic escherichia coli using the polymerase chain reaction (pcr) in patients returning from tropical countries. | we have used the polymerase chain reaction (pcr) to detect shigellae, eiec and etec in stool specimens of diarrhoeic patients returning from tropical countries. as compared to culture (7.1% positive specimens), which recognizes only shigella strains, pcr performed on bacterial growth from directly inoculated macconkey agar plates and directed against virulence-associated genes present in both shigella and eiec was positive in 19.8% of the samples. we assumed that these additional positive result ... | 1994 | 7870070 |
| incidence of motile aeromonas spp. in foods. | a total of 80 food samples were purchased from local retail consumer shops and examined for the presence of motile aeromonas spp. of the food categories tested, poultry had the highest incidence, with 100% positive. this was followed by lamb samples, with 60% positive. raw milk and cheese samples had very low incidence (20%). no motile aeromonas spp. were found in pre-prepared salads. shellfish, fish, pork and beef samples had incidences of 40%. most of the strains isolated were aeromonas hydrop ... | 1994 | 7873101 |
| an example of the stages in the development of a predictive mathematical model for microbial growth: the effects of nacl, ph and temperature on the growth of aeromonas hydrophila. | the stages involved in developing a predictive model are illustrated using data describing the effects of temperature (3-20 degrees c), nacl concentration (0.5-4.5% w/v) and ph (4.6-7.0) on the aerobic growth of aeromonas hydrophila (cocktail of 6 strains). optical density measurements using micro-titre plates were used as an initial screen, to determine the appropriate sampling times for viable counts to be made and to determine the approximate boundaries for growth. growth curves were generate ... | 1994 | 7873337 |
| degradation of propanil by bacterial isolates and mixed populations from a pristine lake. | the microbial transformation rates of propanil, a commonly used herbicide, were investigated using water from a pristine lake in northeast georgia. microbial degradation rates were measured using natural water microflora, the natural water microflora amended with five bacterial species (aerobacter aerogenes, aeromonas hydrophila, acinetobacter calcoaceticus, proteus mirabilis, and aeromonas salmonicida) isolated from the same lake, and the five isolates individually. transformation rate constant ... | 1995 | 7874462 |
| phagocytic defence mechanism in sea bass (dicentrarchus labrax l.): an ultrastructural study. | the ultrastructure of the phagocytic process in fish has not been established in spite of the significant morphofunctional differences detected in the fish immune system with respect to the basic immunological pattern in vertebrates. we report the ultrastructure of the bacterial phagocytic defence mechanism in sea bass (dicentrarchus labrax l.). | 1994 | 7879910 |
| aeromonas caviae exhibits aggregative adherence to hep-2 cells. | | 1994 | 7880270 |
| isolation and characterization of the prolyl aminopeptidase gene (pap) from aeromonas sobria: comparison with the bacillus coagulans enzyme. | the aeromonas sobria pap gene encoding prolyl aminopeptidase (pap) was cloned. it consists of 425 codons and encodes a homotetrameric enzyme of 205 kda. the purified enzyme showed an almost absolute specificity for amino-terminal proline. proline and hydroxyproline residues from many peptide and amide substrates could be easily removed, while no activity was detected for substrates having other amino terminals. the enzyme was very similar to that from bacillus coagulans in many aspects, such as ... | 1994 | 7883756 |
| case of aeromonas veronii (dna group 10) bacteremia. | we describe the first case report of bacteremia due to aeromonas veronii biotype veronii. the infection occurred in a 77-year-old man suffering from multiple underlying conditions which included cancer of the sigmoid colon. because of the unusual biochemical phenotype of this group (ornithine decarboxylase positive), it was originally identified as vibrio cholerae. | 1994 | 7883912 |
| possible virulence factors involved in bacteraemia caused by aeromonas hydrophila. | eighteen strains of aeromonas hydrophila from patients with bacteraemia were investigated for possible virulence factors. cytotoxin and haemolysin were produced by all strains, whereas cholera toxin-like factor was produced by 33% of strains only. enterotoxin production was not detected. haemagglutination of guinea-pig, fowl and rabbit erythrocytes was demonstrated by 83%, 67% and 61% of strains, respectively. fucose- and mannose-sensitive haemagglutinins were predominant. none of the strains ag ... | 1995 | 7884797 |
| conditions for the production and detection of aeromonas enterotoxins. | | 1994 | 7885245 |
| detection of aerolysin gene in aeromonas strains isolated from drinking water, fish and foods by the polymerase chain reaction. | a polymerase chain reaction (pcr) technique was used to assay the presence of the aerolysin gene in a total of 89 aeromonas hydrophila and a. sobria strains isolated from drinking water, fish and foods. these strains were also characterized for the production of virulence factors such as haemolysin, protease and cytotoxin. the primers used in the pcr targeted a 209-bp fragment of the aer gene coding for the beta-haemolysin and detected template dna only in haemolytic a. hydrophila strains. the c ... | 1995 | 7889729 |
| fatal disease mimicking leptospirosis in a dog, caused by aeromonas hydrophila. | a dog was treated for leptospirosis on clinical and epidemiological arguments. the amoxicillin treatment was not successful. pure culture of aeromonas hydrophila was then obtained from liver and kidney, indicating that the septicemia was due to this bacteria commonly found in waters. | 1995 | 7889733 |
| three beta-lactamases isolated from aeromonas salmonicida, including a carbapenemase not detectable by conventional methods. | the beta-lactamases of seven strains of aeromonas salmonicida subsp. achromogenes resistant to amoxicillin (mic > 1024 mg/l) and responsible for furunculosis in farmed atlantic salmon in scotland were examined to establish the mechanisms of beta-lactam resistance. separation of a cell-free extract on an isoelectric focusing gel stained with the chromogenic cephalosporin nitrocefin showed the presence of two beta-lactamases, one with a pi of 7.9 and the other with a pi of 6.0. hydrolysis assays o ... | 1994 | 7889949 |
| identification of the hopg gene, a component of escherichia coli k-12 type ii export system, and its conservation among different pathogenic escherichia coli and shigella isolates. | the escherichia coli k-12 gene coding for a component of a type ii export system was identified and characterized. the hopg protein contains a typical prepilin peptidase cleavage site and has a high degree of homology with proteins pulg, outg, and exeg, which are components of type ii secretion systems from klebsiella pneumoniae, erwinia carotovora, and aeromonas hydrophila. | 1995 | 7896718 |
| prolyl aminopeptidase is not a sulfhydryl enzyme: identification of the active serine residue by site-directed mutagenesis. | prolyl aminopeptidase (pap) has been classified as a sulfhydryl enzyme on the basis of its high sensitivity to p-chloromercuribenzoate and heavy metals. recently, however, the possibility of pap being instead a serine enzyme has been raised as a result of two observations--the conservation of some residues among the paps hitherto sequenced, and a similarity to some serine hydrolases. this is the first report describing an attempt to identify the active residue by site-directed mutagenesis. the p ... | 1994 | 7896753 |
| [distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms]. | the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ... | 1994 | 7898396 |
| bacterial proteins binding to the mammalian extracellular matrix. | pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e. collagens, laminin, fibronectin or proteoglycans. this review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the biological significance of these interactions. the best-characterized bacterial proteins active in these interactions are the mycobacterial fibronectin-binding proteins, the fibronectin- and the col ... | 1993 | 7901732 |
| aeromonas-associated gastroenteritis in egypt. | aeromonas spp. including a. hydrophila, a. sobria, and a. caviae, were recovered from the feces of 88% of diarrheic egyptian children. in contrast, only 45% of nondiarrheic children contained aeromonas spp. a probable source of aeromonas spp. is from drinking water inasmuch as nine out of ten samples analysed from the district of cairo in which the children resided tested positive for aeromonas spp. enterotoxigenicity of the isolates from various sources was tested. 33% of the diarrheic samples ... | 1993 | 7901953 |
| the growth and expression of virulence factors at refrigeration temperature by aeromonas strains isolated from foods. | a potentially significant subset (10%, 6/61) of aeromonas strains isolated from food (milk, lamb, chicken, seafood), all a. veronii biotype sobria, were able to produce two or more exotoxins (haemolysin, enterotoxin, and cytotoxin) at 37 degrees c, and grow well at 43 degrees c. although mesophilic organisms, they grew at 5 degrees c. in addition, they could adhere to hep-2 cells when grown at 37 degrees c, or at 5 degrees c, and expressed flexible pili (possible colonization factors) in greater ... | 1993 | 7906137 |
| physiological consequences of the s-layer of aeromonas salmonicida in relation to growth, temperature, and outer membrane permeation. | s-layers are paracrystalline protein multimers that cover the entire cell surface of many bacterial species. the presence of an s-layer in aeromonas salmonicida (also known as a-layer) predisposed this bacterium to apparently unrelated physiological consequences: inhibition of growth at 30 degrees c, enhanced cell filamentation at 37 degrees c, and enhanced uptake of the hydrophobic antibiotics streptonigrin and chloramphenicol. growth inhibition or enhanced filamentation was not observed when t ... | 1994 | 7922885 |
| toxin production, adherence and protein expression by clinical aeromonas spp. isolates in broth and human pooled ileostomy fluid. | the physiological behaviour of clinical aeromonas spp. isolates was compared following culture in a conventional broth and human pooled ileostomy fluid (pif). protein expression was markedly affected by the growth medium, with an overall reduction in whole cell proteins in bacteria grown in ileostomy fluid. in addition, novel outer membrane proteins were produced in pif but not in broth. the majority of a. hydrophila and a. sobria isolates produced toxin in both broth and pif, whereas no cytotox ... | 1994 | 7925662 |
| etiology and outcome of diarrhea after marrow transplantation: a prospective study. | acute diarrhea after marrow transplant is usually ascribed to acute graft-vs.-host disease (gvhd) or infection, with a reported 40%-50% incidence of infection. the aim of this study was to determine the incidence of acute diarrhea after transplantation, its causes, and its outcome. | 1994 | 7926504 |
| characterization of aeromonas salmonicida subsp. salmonicida: a comparative study of strains of different geographic origin. | a total of 130 strains of the fish pathogen aeromonas salmonicida isolated in denmark, norway, scotland, canada and the usa were examined. the strains originated from farmed salmonid fish. the biochemical, physiological and serological characteristics, antibiotic resistance patterns and cell surface-related properties were compared. aeromonas salmonicida was found to be remarkably consistent in general cultural and biochemical characteristics. it is noteworthy that the strains were positive in t ... | 1994 | 7928780 |
| evaluation of different antimicrobial agents used as selective supplements for isolation and recovery of yersinia enterocolitica. | seventeen antimicrobial agents were evaluated separately or in combination for their efficiency as selective supplements in a broth medium against six different serotypes of yersinia enterocolitica and 20 selected strains of different gram-negative bacteria. irgasan (dp300, 5-chloro-2-(2,4- dichlorophenoxy) phenol) at a concentration of 4 micrograms ml-1 inhibited the growth of most gram-negative bacteria with the exceptions of aeromonas hydrophila, morganella morganii, pseudomonas aeruginosa an ... | 1994 | 7928783 |
| factors influencing beta-galactosidase activity of aeromonas caviae. | aeromonas caviae, often reported to be associated with diarrhoeal patients, elaborates several virulence factors as well as catabolic enzymes such as xylanase and beta-galactosidase. studies on the kinetics of growth of a. caviae and synthesis of beta-galactosidase suggested that the activity was cell associated and reached a peak during the late logarithmic phase of growth. the optimum ph for beta-galactosidase activity was 7.0 and required ca2+ and glutathione for enhancement of its activity; ... | 1994 | 7932108 |
| isolation of aeromonas salmonicida from paddlefish, polyodon spathula. | aeromonas salmonicida was isolated from paddlefish (polyodon spathula) mortalities collected during an epizootic of furunculosis at the spring river state hatchery, arkansas (usa), in 1992. isolates of the bacterium were obtained from culture of gill and kidney tissue. this is the first epizootic of bacterial etiology to be reported in paddlefish. | 1994 | 7933294 |
| unique fimbriae-like structures encoded by sefd of the sef14 fimbrial gene cluster of salmonella enteritidis. | the sef14 gene cluster of salmonella enteritidis was recently shown to contain three genes, sefabc, encoding a unique fimbrin, and proteins homologous to fimbrial chaperones and outer membrane proteins (ushers), respectively. a fourth open reading frame, designated sefd, was found immediately downstream of sefabc and overlapping sefc. the translated protein sequence of sefd was unique, but the composition was similar to that of other bacterial fimbriae. sefd was produced in abundance by wild-typ ... | 1994 | 7934897 |
| spring viraemia of carp. | | 1994 | 7941273 |
| genetic variation in the humoral immune response in atlantic salmon (salmo salar) against aeromonas salmonicida a-layer. | antibody responses to aeromonas salmonicida a-layer were analysed in family material of atlantic salmon (salmo salar), consisting of 791 fish belonging to 34 full-sib groups within 12 paternal half-sib groups. the fish were immunized twice and blood samples were collected three times. significant increase in antibody titre from first to second, and from second to third sampling, was observed. genetic variation in antibody titres was observed at the three samplings with estimated heritabilities r ... | 1994 | 7941312 |
| aeromonas infection and intramural intestinal hemorrhage as a cause of small bowel obstruction. | | 1994 | 7942696 |
| corneal ulcer caused by aeromonas species. | | 1994 | 7943138 |
| aeromonas species in septicemia: laboratory characteristics and clinical observations. | we retrospectively analyzed clinical and epidemiological data on and laboratory characteristics of 53 cases of aeromonas septicemia. only four aeromonas genomospecies (species defined by dna relatedness) were associated with the 53 cases, with aeromonas hydrophila (sensu stricto) predominating (47%). nearly 60% of all aeromonas isolates from blood fell into one of four somatic groups: serogroups o:11, o:16, o:18, and o:34. unlike aeromonas-associated gastroenteritis, septicemia did not peak in f ... | 1994 | 7948561 |
| in-vitro activity of four new fluoroquinolones. | the in-vitro activities of four new fluoroquinolones, e-4749, e-4874, e-4884 and e-4904, were compared with that of ciprofloxacin and sparfloxacin against 1106 clinical isolates. against majority of enterobacteriaceae, general antibacterial activities of e-4749 (mic90s 0.06-1 mg/l), e-4874 (mic90s, 0.03-0.25 mg/l) and e-4884 (mic90s 0.01-0.5 mg/l) were comparable or slightly lower than those of ciproloxacin (mic90s 0.01-0.25 mg/l) and sparfloxacin (mic90s 0.01-1 mg/l). the activity of e-4904 (mi ... | 1994 | 7961215 |
| isolation and characterization of a second exe operon required for extracellular protein secretion in aeromonas hydrophila. | strain c5.84 is a tn5-751 insertion mutant of aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (b. jiang and s.p. howard, j. bacteriol. 173:1241-1249, 1991). a 3.5-kb bglii fragment which complements this mutation was isolated from the chromosome of the parent strain. analysis of this fragment revealed an operon-like structure with two complete genes, exea and exeb, a functional promoter 5' to the exea gene, and a 13-bp inverted r ... | 1994 | 7961440 |
| oral challenge with aeromonas in protein-malnourished mice. | the lack of an animal model for aeromonas-associated diarrhoea has hindered progress toward understanding the pathogenesis of this potentially important enteric infection. protein-malnourished mice were challenged orally with aeromonas strains to determine if diminished levels of resistance would allow the induction of a diarrhoeal response. the 15 aeromonas spp. faecal isolates used for challenge included 7 a. caviae, 4 a. hydrophila, 1 a. sobria bv. sobria, and 1 a. sobria bv. veronii from pat ... | 1994 | 7963338 |
| modulation of the bacterial clearance activity of haemocytes from the freshwater mollusc, lymnaea stagnalis, by the avian schistosome, trichobilharzia ocellata. | the ability of haemocytes, from the haemolymph of the gastropod mollusc lymnaea stagnalis, to recognize and eliminate the bacterium aeromonas salmonicida was shown using an in vitro bacterial clearance assay. the assay employs a dye which is reduced by a. salmonicida in direct proportion to the number of viable bacteria resulting in a colour change which can be determined spectrophotometrically. addition of cytochalasin b resulted in a marked decrease in bacterial clearance, implicating both int ... | 1994 | 7970887 |
| bacterial populations in the groundwater on the us-mexico border in el paso county, texas. | in el paso county, texas, 73 domestic wells were sampled using a variety of selective media to determine the extent of bacterial contamination of the groundwater on the us side of the united states-mexico border. thirteen wells were contaminated by fecal coliforms, whereas other wells contained a variety of bacterial genera including some potential pathogens that normally would not be detected by standard methods of water testing. | 1994 | 7973920 |
| antigen-induced release of macrophage activating factor from rainbow trout oncorhynchus mykiss leucocytes. | the production of macrophage-activating factor (maf) by rainbow trout, oncorhynchus mykiss, head kidney leucocytes at varying times post-immunisation, with the fish bacterial pathogen, aeromonas salmonicida, was investigated and correlated with head kidney lymphocyte proliferation and serum antibody production. maf production was preceded by lymphocyte proliferation and both responses were highest using whole bacterial cells as the in vitro stimulant. maf production and antibody production incre ... | 1994 | 7975191 |
| in vitro and in situ survivals of bacterial populations added to fresh water environments. | the fate of aeromonas hydrophila, alcaligenes denitrificans, vibrio cholerae non-01, pseudomonas putida and four different isolates of escherichia coli in fresh river water were assessed by using different microcosms (i.e., membrane diffusion chamber and erlenmeyer flask). when water samples were incubated at 16 +/- 1 degrees c, the differences in extent of survival among test bacteria were in general not significant. if the incubation temperature was raised to 29 +/- 1 degrees c, in the in situ ... | 1993 | 7982366 |
| el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens]. | a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ... | 1993 | 7984811 |
| [the identification of aeromonas species isolated in cuba]. | 100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests. | 1993 | 7984818 |
| diarrheal disease in peru after the introduction of cholera. | surveillance was conducted one day each week from december 1992 through may 1993 to determine the clinical features and etiology of diarrhea among a population in a suburban community of lima, peru. patients who had had three or more loose stools during the previous 24 hr were enrolled at a clinic located in the community or at a nearby regional hospital. a total of 143 cases of diarrhea were detected for an overall rate of 7.1 cases per 1,000 population. the enteropathogens isolated were vibrio ... | 1994 | 7985750 |
| resistance of bacterial strains to dry conditions: use of anhydrous silica gel in a desiccation model system. | the viability of 18 bacterial strains desiccated on anhydrous silica gel and stored at a temperature of 22 degrees c for at least 3 months was determined. according to their stability in the dried state, these strains could be classified into three typical groups. group 1, containing gram-positive strains and salmonella serotypes, was marked by a very slow decrease of the concentration of culturable cells from day 14 on (respectively day 21 for salmonella thompson). the rate of decrease expresse ... | 1994 | 7989258 |
| the vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms. | the physiological significance of the occurrence of sequence similar antimicrobial peptides in frog skin, as the bombinins in bombina, the magainins in xenopus, and the dermaseptins in phyllomedusa, is a major unanswered question. dermaseptins s1, s2, s3, s4, and s5, a family of cationic (lysine-rich), amphipathic antifungal peptides of 28-34 residues were thus synthesized, purified to homogeneity, and evaluated for their growth-inhibition activity in vitro against various pathogenic microorgani ... | 1994 | 7989335 |
| atypical aeromonas salmonicida isolated from ulcerated chub leuciscus cephalis. | | 1994 | 7992477 |
| isolation and characterization of aeromonas from seafoods in taipei. | a total of 124 fresh seafoods and 158 processed seafoods collected from the retail markets and supermarkets in taipei were tested for the contamination with motile aeromonas spp. of the fresh seafoods analyzed, 88% displayed the presence of aeromonas. the isolation rates of various samples were as follows: 100%, freshwater fish; 95%, seawater fish; 78%, fish fillets; 84%, shrimp and crab of the crustacea group; 83%, bivalve shellfish and 84%, non-bivalve shellfish of the mollusca group, and 100% ... | 1993 | 7995079 |
| the susceptibility of s-layer-positive and s-layer-negative aeromonas strains to complement-mediated lysis. | forty strains of aeromonas hydrophila and aeromonas veronii recovered from invasive and non-invasive infections were tested for their susceptibility to complement-mediated lysis by 65% pooled human serum (phs). based upon the results of this assay, two major populations could be defined. the first group (n = 20) consisted of serogroup o:11 strains, all of which possessed a paracrystalline surface layer (s layer); all of these strains were refractory to the bactericidal activity of 65% phs with t ... | 1994 | 8000552 |
| characterization of aeromonas genomic species by using quinone, polyamine, and fatty acid patterns. | a chemotaxonomic study was carried out on representative strains of 13 aeromonas genomic species. quinone, polyamine, and fatty acid patterns were found to be very useful for an improved characterization of the genus and an improved differentiation from members of the families enterobacteriaceae and vibrionaceae. the q-8-benzoquinone was the predominant ubiquinone, and putrescine and diaminopropane were the major polyamines of the genus. the fatty acid patterns of 181 strains, all characterized ... | 1994 | 8000962 |
| differential elimination of enteric bacteria by protists in a freshwater system. | the short-term (1 h) and long-term (3 d) elimination of low and high densities of five enteric bacteria, klebsiella pneumoniae, aeromonas hydrophila, escherichia coli, enterococcus faecalis and staphylococcus epidermidis, by flagellate and ciliate protists were measured in a freshwater system. in addition, the two processes, ingestion and digestion, which cause the disappearance of those enteric bacteria as time passes, were quantified. the results showed that the elimination of these enteric ba ... | 1994 | 8002473 |
| characteristics of 'atypical', cytochrome oxidase-negative aeromonas salmonicida isolated from ulcerated flounders (platichthys flesus (l.)). | 'atypical', cytochrome oxidase-negative variants of the fish pathogen aeromonas salmonicida, isolated from ulcerated flounder (platichthys flesus), were studied using different methods. two of the strains possessed a protein that corresponded to the a-layer protein of aer. salmonicida. the strains reacted with antibodies against the a-layer and monoclonal antibodies against the o-antigen of typical aer. salmonicida. these tests confirm that the isolates from flounder should be classified as aer. ... | 1994 | 8005836 |
| [sensitivity of different species from the mesophilic aeromonas group isolated from feces to different ph values. clinical significance in the pediatric population]. | | 1994 | 8011731 |
| empyema due to aeromonas hydrophila. | | 1994 | 8011840 |
| maltose transport in aeromonas hydrophila: purification, biochemical characterization and partial protein sequence analysis of a periplasmic maltose-binding protein. | a clinical isolate of aeromonas hydrophila was demonstrated to transport [14c]maltose with similar kinetics to enteric bacteria (km: 0.3 microm; vmax: 22 nmol min-1 per 10(9) cells). the uptake of [14c]maltose was completely inhibited in the presence of unlabelled maltose or maltodextrins, whereas other mono- and disaccharides, such as glucose, galactose, sucrose, lactose or melibiose, had no effect. a protein with an apparent molecular mass of 39 kda (maltose-binding protein; mbp) was identifie ... | 1994 | 8012611 |
| effect of growth temperature on complement-mediated killing of mesophilic aeromonas spp. serotype o:34. | mesophilic aeromonas spp. strains (serotype o:34) showed sensitivity to complement-mediated killing when they were cultivated at 37 degrees c (serum-sensitive) but not when they were cultivated at 20 degrees c (serum-resistant). these strains produced smooth lipopolysaccharide when they were grown at 20 degrees c and rough lipolysaccharide when cultivated at 37 degrees c. the reason for the resistance to complement-mediated killing could be that c3b is rapidly degraded (possibly because it is bo ... | 1994 | 8013874 |
| production of dogik: an improved ogi (nigerian fermented weaning food) with potentials for use in diarrhoea control. | as part of a programme to formulate foods to aid the control of diarrhoeal diseases, an improved ogi (the commonest weaning food in nigeria) named dogik has been developed. dogik was produced by using lactobacillus starter cultures with antimicrobial activity against diarrhoeagenic bacteria and also possessing amylolytic activity. the survival of diarrhoeagenic bacteria was investigated in locally-fermented ogi and in dogik. the foods were inoculated with cell suspensions of salmonella, shigella ... | 1994 | 8015024 |
| studies on the toxic and immunosuppressive effects of cadmium on the common carp. | in the last couple of decades, research on cadmium extended from its toxicologic aspects to its role in environmental pollution. the toxicity of cadmium to different species of fish varies widely. the cadmium sensitivity of batches of common carp, the most important cultured fish species of hungary, was studied. lethality, histopathologic changes and effect of cadmium on the antibody response, lysozyme level and microbicidal capacity were monitored. wide variation was found in individual sensiti ... | 1993 | 8017244 |
| comparison of the microbiological quality of water coolers and that of municipal water systems. | the microbiological quality of tap water and that of water from 50 water coolers located in residences and workplaces were comparatively studied. in addition, difference factors that might influence the bacteriological contamination of water dispensers were examined. aeorbic and facultative anaerobic heterotrophic bacteria, total coliforms, and two indicators for fecal contamination (fecal coliforms and fecal streptococci) as well as three types of pathogenic bacteria (staphylococcus aureus, pse ... | 1994 | 8017912 |
| comparison of putative virulence factors in aeromonas hydrophila strains isolated from the marine environment and human diarrheal cases in southern italy. | aeromonas hydrophila strains isolated from the same geographical region (southern italy) but from different sources (sea sediments and human diarrhea cases) were characterized for the production of potential virulence determinants, such as production of cytotoxins, cytotonic toxins, hemolysin, and dermonecrotic factors and their capacity to adhere to human intestinal 407 cells in vitro. the results showed that isolates from both the sources produced all or some of the virulence factors which may ... | 1994 | 8017924 |
| sequence analysis of the l1 metallo-beta-lactamase from xanthomonas maltophilia. | the amino acid sequence deduced from the l1 beta-lactamase gene of xanthomonas maltophilia shows a significant variation from that of the cpha and blm metallo-beta-lactamases of aeromonas hydrophila and bacillus cereus, respectively. whilst the n-terminus of the l1 protein shows some similarity, particularly at the histidine residues previously suggested as a zinc-binding motif, the c-terminus of the protein demonstrates very little similarity. such differences amongst this group of enzymes woul ... | 1994 | 8018721 |
| [biochemical identification of aeromonads]. | to assess the species of the genus aeromonas in 178 strains isolated from human and animal materials and from the environment (water and hospital environment), examined along with 11 type strains of the best known species, a key was used the basis of which are in addition to biochemical tests defining the genus aeromonas 12 tests: voges-proskauer, lysine decarboxylase, gas from glucose, haemolysis, gluconate oxidation, elastase, arabinose, mannose, saccharose, salicine, esculine hydrolysis, arbu ... | 1994 | 8019811 |
| [identification of aeromonads from water sources]. | a total of 102 aeromonas strains isolated from water were identified by both commercial enterotest 1 & 2 kits and by several conventional tests. 83 strains (81.4%) were identified to the species level according to a differentiation table. a. hydrophila (36 strains), a. caviae (26 strains) and a. sobria (10 strains) species were isolated most frequently. strains identified as a. allosaccharophila, a. eucrenophila, a. jandaei, a. media and a. trota were very rare. the remaining 19 strains could no ... | 1994 | 8019812 |
| survival and recovery of aeromonas hydrophila in water: development of methodology for testing bottled water in canada. | proposed changes to the regulations for bottled water in the food and drugs act of canada include criteria for aeromonas hydrophila (0 colony-forming units/100 ml water). the development of the methodology used to support these proposed regulations and the survival of a. hydrophila in inoculated water are described. the methodology used in the isolation of a. hydrophila includes the use of hydrophobic grid membrane filters (hgmf), a resuscitation step on tryptic soy agar, and selective plating o ... | 1994 | 8019937 |
| aeromonas species exhibit aggregative adherence to hep-2 cells. | clinical and environmental isolates of aeromonas species (five a. hydrophila isolates, three a. caviae isolates, and two a. sobria isolates) were tested for their adherence to hep-2 cells. clinical isolates of a. hydrophila and a. sobria exhibited aggregative adherence similar to that presented by enteroadherent-aggregative escherichia coli. bacterial aggregates adhered to cells with a typical "stacked-brick" appearance. in contrast, a. caviae strains showed a diffuse adherence pattern. | 1994 | 8027331 |
| colonic carcinoma manifesting as aeromonas colitis. | | 1994 | 8034927 |
| [the biology of bacteria used to treat industrial sewage to remove heavy metals]. | the content of heavy metals in the water bodies increases as a result of anthropogenic effects. heavy metals exert toxical effect on hydrobionts. involved into the trophic chain they can be the cause of grave human diseases. microorganisms possess a capacity to accumulate heavy metals in their body, evoke the process of their enzymatic transformation into nontoxical compounds and adsorb them on the cell surface. when breeding bacterial strains for the biological treatment of sewage from the meta ... | 1993 | 8037799 |
| in-vitro evaluation of cefpodoxime. | in-vitro antimicrobial activity of cefpodoxime was evaluated against several microbial species by both conventional tests and additional parameters which take into consideration some of the conditions likely to be encountered in infected tissues. mics for 414 recent clinical isolates, including staphylococci, streptococci, haemophilus influenzae, moraxella catarrhalis, several enterobacteriaceae, aeromonas hydrophila and campylobacter jejuni were determined. mic values overall were similar to th ... | 1994 | 8040114 |
| diversity of siderophore genes encoding biosynthesis of 2,3-dihydroxybenzoic acid in aeromonas spp. | most species of the genus aeromonas produce the siderophore amonabactin, although two species produce enterobactin, the siderophore of many enteric bacteria. both siderophores contain 2,3-dihydroxybenzoic acid (2,3-dhb). siderophore genes (designated aebc, -e, -b and -a, for aeromonad enterobactin biosynthesis) that complemented mutations in the enterobactin genes of the escherichia coli 2,3-dhb operon, entceba(p15), were cloned from an enterobactin-producing isolate of the aeromonas spp. mappin ... | 1994 | 8043988 |
| [activity of lysosomal proteinases in carb tissue in aeromonas infection]. | the activity of major lysosomal proteinases (cathepsins b and d) increased in carp tissues infested with aeromonas hydrophila. the value and character of changes in the activity of cathepsins depended on the degree of infestation and the physiological state of fishes (normally fed or starved). | 1994 | 8047538 |
| [heterotrophic aerobic gram negative bacilli from the itata fluvial system, chile]. | total bacterial counts, counts of gram negative bacilli and faecal pollution were determined in samples of freshwater collected in different sampling sites of the fluvial system itata, in the eight region of chile. these stations cover the course of the system from the andes down to the pacific ocean. bacterial counts were rather low and varied according to the human population density in the sampling sites. most of the identified bacilli were nonfermenting bacilli, among which the highest numbe ... | 1993 | 8047731 |
| isolation and characterization of a tn5-induced tolq mutant of escherichia coli. | transposon mutagenesis was used to isolate an escherichia coli mutant that released into the external medium a heterologous protein, the aeromonas hydrophila aerolysin. genetic mapping and phenotypic characterization of e. coli cm209 showed that tn5 is inserted in the tolq gene. this mutant strain released a significant amount of unprocessed (proaerolysin) protein into the external medium, together with other periplasmic proteins. similar levels of the toxin were detected in the intracellular co ... | 1994 | 8050069 |
| the transposon-like structure of is26-tetracycline, and kanamycin resistance determinant derived from transferable r plasmid of fish pathogen, pasteurella piscicida. | tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable r plasmid of fish pathogen pasteurella piscicida, and complete nucleotide sequences were determined. the pp-tet was a class d tet determinant constructed with the teta resistance gene of 1,182 bp encoding a protein with a deduced molecular mass of 41 kda and the tetr repressor gene of 654 bp encoding a product of 24 kda. the pp-tet was highly homologous to the tet(d) of plasmid ra1 isolated from aeromo ... | 1994 | 8052160 |
| aeromonas peritonitis. | five new cases of peritonitis caused by aeromonas species are reported, and 29 others described in the literature are reviewed. males predominated (71%), and the mean age was 56.9 years. acquisition was nosocomial in 20% of the cases. all patients except one (3%) had significant underlying diseases; 73% had chronic hepatic disease, 15% had chronic renal failure (treated with chronic ambulatory peritoneal dialysis [capd]), and 9% had an intestinal perforation. symptoms were similar to those of pe ... | 1994 | 8054435 |
| [aeromonads and their sensitivity to antibacterial drugs (review of the foreign literature 1985-1992)]. | the review is concerned with the modern studies on the taxonomy of aeromonades and their role in the development of infectious complications of various localization, including intestinal infections. a special attention is paid to antibiotic resistance of aeromonades isolated from pathological materials and the modern notions of the factors of aeromonade pathogenicity. | 1994 | 8060198 |
| dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. | immunostimulant preparations macrogard, candida utilis, saccharomyces cerevisiae, evetsel, chitosan, or finnstim were mixed into semipurified diets and fed to groups of rainbow trout for 1 week. fish were bled by non-lethal caudal puncture and blood samples assayed for changes in non-specific cellular immunity and humoral protein levels. in the immunostimulated fish, hematocrit levels and lymphocyte counts remained relatively stable; however, elevations were observed in oxidative radical release ... | 1994 | 8066989 |
| a beta-glucan inhibitable zymosan receptor on channel catfish neutrophils. | in mice and humans zymosan binds to the complement receptor three/mac-1 receptor; however, identification of this receptor in channel catfish (ictalurus punctatus) has not been accomplished. soluble fluorescein isothiocyanate (fitc) conjugated beta-glucan, a component of zymosan, was found to bind to channel catfish anterior kidney (ak) neutrophils but not to b-lymphocytes. serum activated zymosan (saz)-mediated chemiluminescence responses of channel catfish ak neutrophils could be inhibited by ... | 1994 | 8066990 |
| adaptive acid tolerance response (atr) in aeromonas hydrophila. | aeromonas hydrophila, a gastrointestinal pathogen of humans, was shown to exhibit a significant adaptive acid tolerance response (atr) capable of protecting cells from severe acid at a ph of 3.5. the atr was induced by exposure to a relatively mild ph level of 5.0 for 20 min. adaptation required protein synthesis since treatment with chloramphenicol during adaptation to ph 5.0 prevented the development of acid tolerance. the adaptation to acid environment was found to be a non-transient phenomen ... | 1994 | 8075809 |
| two-year study of endemic enteric pathogens associated with acute diarrhea in new caledonia. | a longitudinal study of diarrheal disease among patients of all ages with acute diarrhea was carried out in new caledonia from january 1990 to december 1991. stool samples from 2,088 diarrheal patients were examined for parasites, rotavirus, and bacterial pathogens. potential sources of contamination (drinking water, seawater and bovine and porcine feces) were investigated. one or more enteric pathogens were identified in 41.8 and 40.6% of the persons with diarrhea, in 1990 and 1991, respectivel ... | 1994 | 8077399 |
| rapid biochemical screening for salmonella, shigella, yersinia, and aeromonas isolates from stool specimens. | four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. the salmonella screen consisted of kligler iron agar (kia), motility-indole-urea-tryptophan-deamination semisolid medium (miu-tda), and the o-nitrophenyl-beta-d-galactopyranoside (onpg) test; the shigella screen consisted of kia, miu-tda, the onpg test, and the lysine decarboxylation-indole test; the yersinia screen consisted of a rhamnose broth; the aeromonas screen consisted of ... | 1994 | 8077408 |
| pyrolysis mass spectrometry characterisation and numerical taxonomy of aeromonas spp. | reference strains (2) and 29 isolates of aeromonas spp. from clinical material and environmental specimens were characterised in traditional biochemical tests, and in pyrolysis mass spectrometry, which gives data reflecting whole-cell composition. numerical taxonomic analyses of the data sets were compared with conventional identification at species level, and pathogenic potential, as inferred from the origin of the isolates. clustering with conventional test reaction patterns showed, for each o ... | 1993 | 8085793 |
| [the comparative characteristics of the antibiotic sensitivity of psychrophilic and mesophilic aeromonads]. | the general regularities of the antibiotic susceptibility of psychrophilic and mesophilic aeromonads were determined. the antibioticograms were in general similar. still, there was observed a higher susceptibility of aeromonas salmonicida to tetracycline, chloramphenicol and rifampicin, as well as a larger number of strains susceptible to semisynthetic broad spectrum penicillins (ampicillin and carbenicillin) and cephazoline. the susceptibility to aminoglycosides was lower. | 1993 | 8085887 |
| crystal structure of aeromonas proteolytica aminopeptidase: a prototypical member of the co-catalytic zinc enzyme family. | aminopeptidases specifically cleave the amino-terminal residue from polypeptide chains and are involved in the metabolism of biologically active peptides. the family includes zinc-dependent enzymes possessing either one or two zinc ions per active site. structural studies providing a detailed view of the metal environment may reveal whether the one-zinc and two-zinc enzymes constitute structurally and mechanistically distinct subclasses, and what role the metal ions play in the catalytic process ... | 1994 | 8087555 |
| molecular cloning and characterization of prolyl endopeptidase from human t cells. | the prolyl endopeptidase (pep) gene of human t cells was amplified by the pcr method and cloned in escherichia coli. the complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750. the nucleotide sequence of this clone revealed that t cell pep dna is 48, 50, and 91% homologous to those of flavobacterium meningosepticum, aeromonas hydrophila, and porcine brain pep, respectively. this gene was fused to the lacz sequence from e. ... | 1994 | 8089089 |
| assessment of a chemiluminescent universal probe for taxonomical and epidemiological investigations of aeromonas sp isolates. | to assess a chemiluminescent universal probe for taxonomical and epidemiological investigations of aeromonas sp isolates. | 1994 | 8089222 |
| bacteriological investigation of the occurrence and antibiotic sensitivities of the gut-flora of the potential southern african medicinal leech, asiaticobdella buntonensis (hirudinidae). | the lack of availability of medicinal leeches is a major impediment to the widespread use of leech therapy for treatment of congested flaps and replants in southern africa. an investigation into the suitability of an alternative leech, the indigenous southern african leech, asiaticobdella buntonensis, was therefore started. the risk of hospital-acquired infection related to the use of leeches and the antibiotic sensitivities of bacteria isolated from the gastro-intestinal tract of wild-caught le ... | 1993 | 8099096 |
| the effect of bacteria infection on mean selected body temperature in the common agama, agama agama: a dose-response study. | 1. the fever response was studied in 43 common agamas using a self-pairing experiment in which animals received an intraperitoneal injection of sterile saline and an injection of one of six dosages of dead aeromonas sobria (1 x 10(6), 1 x 10(7), 1 x 10(8), 1 x 10(9), 1 x 10(10), and 1 x 10(11) total organisms). 2. the results demonstrated a significant increase in tb (1.6-3.1 degrees c) above the mean selected body temperature (msbt) of the saline injection animals over a bacteria infection rang ... | 1993 | 8101779 |
| pili and the interaction of aeromonas species with human peripheral blood polymorphonuclear cells. | the interaction of differentially piliated aeromonas strains expressing pili of two broadly different morphologic types (short, rigid (s/r) and/or long, wavy (l/w)) with human peripheral blood mononuclear leukocytes (pmn) was investigated to determine whether host defense cells might exert a selective pressure on pili expression in vivo accounting for the different pili phenotypes of clinical and environmental strains. a majority of aeromonas veronii biotype sobria strains from water (6/6) and f ... | 1993 | 8104626 |
| the public health significance of aeromonas spp. in foods. | there is now evidence that some strains of aeromonas species are enteropathogens. such strains possess virulence properties, such as the ability to produce enterotoxins, cytotoxins, haemolysins and/or the ability to invade epithelial cells. strains with these properties are common contaminants of drinking water and a wide range of foods. contact or consumption of contaminated water, especially in summer, is a major risk factor in aeromonas-associated gastroenteritis. aeromonas-contaminated foods ... | 1993 | 8110597 |
| meningitis due to aeromonas species: case report and review. | in recent years, aeromonas species has been reported to cause extraintestinal infections with a growing frequency. meningitis due to aeromonas species is, however, a rare entity. we report a case of aeromonas meningitis in a 54-year-old man with a history of chronic alcoholic liver disease who, after an episode of gastroenteritis, developed an acute clinical picture characteristic of meningitis with septic shock and ecthyma gangrenosum. aeromonas veronii (biogroup sobria) was isolated from cultu ... | 1993 | 8110931 |
| wound infection due to aeromonas sobria. | | 1993 | 8110950 |
| transfer of plasmid rp4 from escherichia coli k-12 to indigenous bacteria of seawater. | transfer of plasmid rp4 from escherichia coli k-12 donor strain to bacteria isolated from seawater was shown to occur on filters and in sterile seawater incubated at 24 degrees c. ten of 12 seawater isolates tested were recipient active for rp4 when the plasmid transfers were assessed by filter matings. when matings were performed in sterile seawater, seven of the 12 isolates received rp4. in sterile seawater, the transfer of rp4 from e. coli to pseudomonads was more efficient than transfer betw ... | 1993 | 8111533 |
| cloning and expression of putative cytotonic enterotoxin-encoding genes from aeromonas hydrophila. | a genomic library from a diarrheal isolate, ssu, of aeromonas hydrophila was constructed in a cosmid vector, phc79, and in bacteriophage lambda embl3. cell lysates from various escherichia coli clones containing the recombinant cosmid were examined for their ability to elongate chinese hamster ovary (cho) cells, which is a typical enterotoxic response. based on restriction analysis, a 4.0-kb sali dna fragment from one of the clones that exhibited enterotoxic activity was subcloned into a bacteri ... | 1994 | 8112594 |
| cytotoxic and haemagglutinating activities of motile aeromonas species. | cytotoxic and haemagglutinating properties were determined in 114 aeromonas strains isolated from various sites in slaughtered lambs and from processed lamb meat. cytotoxic activity on vero cells was observed in 48 (42%) of the strains. it was more common in a. sobria and a. hydrophila isolates than with a. caviae isolates. haemagglutination (ha) activity was found frequently in motile aeromonads irrespective of species; it was present in 50% of a. sobria strains, 51% of a. hydrophila strains an ... | 1994 | 8114068 |